Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050253.t1 | XP_013448450.1 | 80.7 | 109 | 21 | 0 | 41 | 149 | 1 | 109 | 1.10E-39 | 172.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050253.t1 | O03982 | 56.6 | 76 | 32 | 1 | 41 | 115 | 1 | 76 | 4.7e-15 | 82.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050253.t1 | A0A072TYH4 | 80.7 | 109 | 21 | 0 | 41 | 149 | 1 | 109 | 7.9e-40 | 172.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049350 | MS.gene050253 | 0.806805 | 6.88E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene051676 | 0.864218 | 1.42E-64 | -1.69E-46 |
| MS.gene050253 | MS.gene051700 | 0.809585 | 1.76E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene051719 | 0.803187 | 3.93E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene051720 | 0.813847 | 2.08E-51 | -1.69E-46 |
| MS.gene050253 | MS.gene053581 | 0.802937 | 4.43E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene05423 | 0.810066 | 1.39E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene05458 | 0.800511 | 1.39E-48 | -1.69E-46 |
| MS.gene050253 | MS.gene055313 | 0.808795 | 2.60E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene058526 | 0.811767 | 5.94E-51 | -1.69E-46 |
| MS.gene050253 | MS.gene058527 | 0.810586 | 1.07E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene058528 | 0.812095 | 5.04E-51 | -1.69E-46 |
| MS.gene050253 | MS.gene058852 | 0.813408 | 2.60E-51 | -1.69E-46 |
| MS.gene050253 | MS.gene061564 | 0.81546 | 9.14E-52 | -1.69E-46 |
| MS.gene050253 | MS.gene06322 | 0.808815 | 2.57E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene06324 | 0.803078 | 4.14E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene064243 | 0.800237 | 1.58E-48 | -1.69E-46 |
| MS.gene050253 | MS.gene065072 | 0.805671 | 1.19E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene066477 | 0.8021 | 6.58E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene072116 | 0.802301 | 5.99E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene073275 | 0.814249 | 1.70E-51 | -1.69E-46 |
| MS.gene050253 | MS.gene20621 | 0.809264 | 2.06E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene21103 | 0.804419 | 2.18E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene21105 | 0.804073 | 2.57E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene21107 | 0.804238 | 2.38E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene22177 | 0.818385 | 2.02E-52 | -1.69E-46 |
| MS.gene050253 | MS.gene23697 | 0.81631 | 5.91E-52 | -1.69E-46 |
| MS.gene050253 | MS.gene24493 | 0.815524 | 8.85E-52 | -1.69E-46 |
| MS.gene050253 | MS.gene26033 | 0.870607 | 1.28E-66 | -1.69E-46 |
| MS.gene050253 | MS.gene28509 | 0.80376 | 2.99E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene29794 | 0.806309 | 8.76E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene29807 | 0.808615 | 2.84E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene30663 | 0.801685 | 8.01E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene31045 | 0.822456 | 2.35E-53 | -1.69E-46 |
| MS.gene050253 | MS.gene31650 | 0.800725 | 1.26E-48 | -1.69E-46 |
| MS.gene050253 | MS.gene35530 | 0.829305 | 5.54E-55 | -1.69E-46 |
| MS.gene050253 | MS.gene35575 | 0.808264 | 3.37E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene37570 | 0.800222 | 1.59E-48 | -1.69E-46 |
| MS.gene050253 | MS.gene40969 | 0.815776 | 7.77E-52 | -1.69E-46 |
| MS.gene050253 | MS.gene41757 | 0.829646 | 4.58E-55 | -1.69E-46 |
| MS.gene050253 | MS.gene42653 | 0.800827 | 1.20E-48 | -1.69E-46 |
| MS.gene050253 | MS.gene45281 | 0.817731 | 2.83E-52 | -1.69E-46 |
| MS.gene050253 | MS.gene49876 | 0.806891 | 6.60E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene51384 | 0.808812 | 2.58E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene52420 | 0.807967 | 3.90E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene52808 | 0.805854 | 1.09E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene54766 | 0.806412 | 8.33E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene56476 | 0.801942 | 7.10E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene56510 | 0.803979 | 2.69E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene56565 | 0.815059 | 1.12E-51 | -1.69E-46 |
| MS.gene050253 | MS.gene57576 | 0.812776 | 3.58E-51 | -1.69E-46 |
| MS.gene050253 | MS.gene58227 | 0.801349 | 9.39E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene58467 | 0.802434 | 5.62E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene58468 | 0.802679 | 5.00E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene59241 | 0.809268 | 2.06E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene61257 | 0.809858 | 1.54E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene61720 | 0.80267 | 5.03E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene62504 | 0.80638 | 8.46E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene65172 | 0.805518 | 1.28E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene66476 | 0.863704 | 2.06E-64 | -1.69E-46 |
| MS.gene050253 | MS.gene66596 | 0.816879 | 4.41E-52 | -1.69E-46 |
| MS.gene050253 | MS.gene68998 | 0.810431 | 1.16E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene71879 | 0.807131 | 5.87E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene72376 | 0.806207 | 9.20E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene72621 | 0.837385 | 5.36E-57 | -1.69E-46 |
| MS.gene050253 | MS.gene72986 | 0.808616 | 2.84E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene74606 | 0.808677 | 2.75E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene75413 | 0.806536 | 7.84E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene75719 | 0.801092 | 1.06E-48 | -1.69E-46 |
| MS.gene050253 | MS.gene75781 | 0.800799 | 1.22E-48 | -1.69E-46 |
| MS.gene050253 | MS.gene76248 | 0.808477 | 3.04E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene81831 | 0.833151 | 6.29E-56 | -1.69E-46 |
| MS.gene050253 | MS.gene82600 | 0.803244 | 3.82E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene84426 | 0.808542 | 2.94E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene89488 | 0.802348 | 5.86E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene89605 | 0.813072 | 3.08E-51 | -1.69E-46 |
| MS.gene050253 | MS.gene89812 | 0.813471 | 2.52E-51 | -1.69E-46 |
| MS.gene050253 | MS.gene89985 | 0.803571 | 3.27E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene90097 | 0.805164 | 1.52E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene90605 | 0.804556 | 2.04E-49 | -1.69E-46 |
| MS.gene050253 | MS.gene90649 | 0.806409 | 8.34E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene93985 | 0.832054 | 1.18E-55 | -1.69E-46 |
| MS.gene050253 | MS.gene98827 | 0.832827 | 7.57E-56 | -1.69E-46 |
| MS.gene050253 | MS.gene98848 | 0.809686 | 1.67E-50 | -1.69E-46 |
| MS.gene050253 | MS.gene99848 | 0.802347 | 5.86E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTGGACACTGCCTTTAC+TGG | 0.181442 | 7.3:-53956391 | None:intergenic |
| AAGACAGCATCTAGCCTTTC+AGG | 0.206805 | 7.3:+53955842 | MS.gene050253:CDS |
| TAGGTTTCAATGTATTGAAT+TGG | 0.333492 | 7.3:-53956499 | None:intergenic |
| CAGTGTCCAAGCTAAGAAAA+TGG | 0.399021 | 7.3:+53956401 | MS.gene050253:CDS |
| TTAACAGAAAGTAGTGTTGA+AGG | 0.440878 | 7.3:+53955781 | MS.gene050253:intron |
| TTCTGTTGAATCTCTAAAAG+AGG | 0.445440 | 7.3:+53956441 | MS.gene050253:CDS |
| CGAGAAGAAATTGACATTGA+AGG | 0.460415 | 7.3:+53956357 | MS.gene050253:CDS |
| TAGCTGATTAAGAATTGATA+AGG | 0.508308 | 7.3:-53955573 | None:intergenic |
| GAGAAGAAATTGACATTGAA+GGG | 0.511122 | 7.3:+53956358 | MS.gene050253:CDS |
| GGGGTCATCTGATAGTAATA+CGG | 0.532976 | 7.3:-53956523 | None:intergenic |
| AATCTCGAAAATATTGTGGT+GGG | 0.543098 | 7.3:-53956543 | None:intergenic |
| ACAAACTTCAACAACAATGA+AGG | 0.543745 | 7.3:+53955598 | MS.gene050253:CDS |
| TAATCTCGAAAATATTGTGG+TGG | 0.564645 | 7.3:-53956544 | None:intergenic |
| AAATTCAATATGTACCTGAA+AGG | 0.592630 | 7.3:-53955856 | None:intergenic |
| ATCTCGAAAATATTGTGGTG+GGG | 0.596272 | 7.3:-53956542 | None:intergenic |
| CATCTGATAGTAATACGGAT+AGG | 0.615334 | 7.3:-53956518 | None:intergenic |
| TAGTAATCTCGAAAATATTG+TGG | 0.619288 | 7.3:-53956547 | None:intergenic |
| ACAGAAAGTAGTGTTGAAGG+TGG | 0.627460 | 7.3:+53955784 | MS.gene050253:intron |
| AGAAGAAATTGACATTGAAG+GGG | 0.633714 | 7.3:+53956359 | MS.gene050253:CDS |
| AGTGTGTTTAAGCAATGAAG+TGG | 0.651750 | 7.3:-53955522 | None:intergenic |
| TGAAGTGGTGATATGTAGCA+AGG | 0.666004 | 7.3:-53955507 | None:intergenic |
| GAAGAAATTGACATTGAAGG+GGG | 0.666381 | 7.3:+53956360 | MS.gene050253:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAACTTTATAAAACAATATA+TGG | + | chr7.3:53956142-53956161 | MS.gene050253:intron | 10.0% |
| !!! | ATATATTGTTTTATAAAGTT+AGG | - | chr7.3:53956143-53956162 | None:intergenic | 10.0% |
| !!! | ATTTTTTATTTTTATTTTGC+AGG | + | chr7.3:53956307-53956326 | MS.gene050253:intron | 10.0% |
| !!! | TTTTTTATTTTTATTTTGCA+GGG | + | chr7.3:53956308-53956327 | MS.gene050253:intron | 10.0% |
| !!! | TTTTTATTTTTATTTTGCAG+GGG | + | chr7.3:53956309-53956328 | MS.gene050253:intron | 15.0% |
| !! | AAAAATAAAATCGATCATGA+TGG | - | chr7.3:53956031-53956050 | None:intergenic | 20.0% |
| !! | AAAGGATATAACAACAAAAA+AGG | - | chr7.3:53955710-53955729 | None:intergenic | 20.0% |
| !! | AAGGATATAACAACAAAAAA+GGG | - | chr7.3:53955709-53955728 | None:intergenic | 20.0% |
| !! | CACAAAAGTCAAATTAAATT+AGG | - | chr7.3:53956251-53956270 | None:intergenic | 20.0% |
| !!! | TATCCTTTCATATTTTAGAA+TGG | + | chr7.3:53955722-53955741 | MS.gene050253:intron | 20.0% |
| ! | AAATTCAATATGTACCTGAA+AGG | - | chr7.3:53955859-53955878 | None:intergenic | 25.0% |
| ! | AAGTTGTGTTCAAAAGTTTA+GGG | + | chr7.3:53955930-53955949 | MS.gene050253:intron | 25.0% |
| ! | AATGAATCTTTCGTGTTATA+TGG | - | chr7.3:53956290-53956309 | None:intergenic | 25.0% |
| ! | TAAGTTGTGTTCAAAAGTTT+AGG | + | chr7.3:53955929-53955948 | MS.gene050253:intron | 25.0% |
| ! | TAGCTGATTAAGAATTGATA+AGG | - | chr7.3:53955576-53955595 | None:intergenic | 25.0% |
| ! | TAGGTTTCAATGTATTGAAT+TGG | - | chr7.3:53956502-53956521 | None:intergenic | 25.0% |
| ! | TAGTAATCTCGAAAATATTG+TGG | - | chr7.3:53956550-53956569 | None:intergenic | 25.0% |
| ! | TGAAGATGATAAAAGATTAC+AGG | - | chr7.3:53955999-53956018 | None:intergenic | 25.0% |
| !!! | CTTCCATTCTAAAATATGAA+AGG | - | chr7.3:53955728-53955747 | None:intergenic | 25.0% |
| AATCTCGAAAATATTGTGGT+GGG | - | chr7.3:53956546-53956565 | None:intergenic | 30.0% | |
| ACAAACTTCAACAACAATGA+AGG | + | chr7.3:53955598-53955617 | MS.gene050253:CDS | 30.0% | |
| AGAAGAAATTGACATTGAAG+GGG | + | chr7.3:53956359-53956378 | MS.gene050253:CDS | 30.0% | |
| AGTTGTGTTCAAAAGTTTAG+GGG | + | chr7.3:53955931-53955950 | MS.gene050253:intron | 30.0% | |
| GAGAAGAAATTGACATTGAA+GGG | + | chr7.3:53956358-53956377 | MS.gene050253:CDS | 30.0% | |
| TAATCTCGAAAATATTGTGG+TGG | - | chr7.3:53956547-53956566 | None:intergenic | 30.0% | |
| ! | TTAACAGAAAGTAGTGTTGA+AGG | + | chr7.3:53955781-53955800 | MS.gene050253:intron | 30.0% |
| ! | TTCTGTTGAATCTCTAAAAG+AGG | + | chr7.3:53956441-53956460 | MS.gene050253:CDS | 30.0% |
| !!! | TAGGTTTTATTCTCAAACGA+CGG | - | chr7.3:53956232-53956251 | None:intergenic | 30.0% |
| AGTGTGTTTAAGCAATGAAG+TGG | - | chr7.3:53955525-53955544 | None:intergenic | 35.0% | |
| ATCTCGAAAATATTGTGGTG+GGG | - | chr7.3:53956545-53956564 | None:intergenic | 35.0% | |
| CATCTGATAGTAATACGGAT+AGG | - | chr7.3:53956521-53956540 | None:intergenic | 35.0% | |
| CGAGAAGAAATTGACATTGA+AGG | + | chr7.3:53956357-53956376 | MS.gene050253:CDS | 35.0% | |
| GAAGAAATTGACATTGAAGG+GGG | + | chr7.3:53956360-53956379 | MS.gene050253:CDS | 35.0% | |
| GTTGTGTTCAAAAGTTTAGG+GGG | + | chr7.3:53955932-53955951 | MS.gene050253:intron | 35.0% | |
| TACACTTGCTCAAAACAGAA+AGG | + | chr7.3:53956075-53956094 | MS.gene050253:intron | 35.0% | |
| TTGTGTTCAAAAGTTTAGGG+GGG | + | chr7.3:53955933-53955952 | MS.gene050253:intron | 35.0% | |
| ! | TTGACACCATTTTCTTAGCT+TGG | - | chr7.3:53956410-53956429 | None:intergenic | 35.0% |
| CAGTGTCCAAGCTAAGAAAA+TGG | + | chr7.3:53956401-53956420 | MS.gene050253:CDS | 40.0% | |
| GGGGTCATCTGATAGTAATA+CGG | - | chr7.3:53956526-53956545 | None:intergenic | 40.0% | |
| TGAAGTGGTGATATGTAGCA+AGG | - | chr7.3:53955510-53955529 | None:intergenic | 40.0% | |
| ! | ACAGAAAGTAGTGTTGAAGG+TGG | + | chr7.3:53955784-53955803 | MS.gene050253:intron | 40.0% |
| GGGGGATATTGATCCAGTAA+AGG | + | chr7.3:53956378-53956397 | MS.gene050253:CDS | 45.0% | |
| ! | AAGACAGCATCTAGCCTTTC+AGG | + | chr7.3:53955842-53955861 | MS.gene050253:CDS | 45.0% |
| AGCTTGGACACTGCCTTTAC+TGG | - | chr7.3:53956394-53956413 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 53955493 | 53956579 | 53955493 | ID=MS.gene050253 |
| chr7.3 | mRNA | 53955493 | 53956579 | 53955493 | ID=MS.gene050253.t1;Parent=MS.gene050253 |
| chr7.3 | exon | 53955493 | 53955619 | 53955493 | ID=MS.gene050253.t1.exon1;Parent=MS.gene050253.t1 |
| chr7.3 | CDS | 53955493 | 53955619 | 53955493 | ID=cds.MS.gene050253.t1;Parent=MS.gene050253.t1 |
| chr7.3 | exon | 53955788 | 53955863 | 53955788 | ID=MS.gene050253.t1.exon2;Parent=MS.gene050253.t1 |
| chr7.3 | CDS | 53955788 | 53955863 | 53955788 | ID=cds.MS.gene050253.t1;Parent=MS.gene050253.t1 |
| chr7.3 | exon | 53956329 | 53956579 | 53956329 | ID=MS.gene050253.t1.exon3;Parent=MS.gene050253.t1 |
| chr7.3 | CDS | 53956329 | 53956579 | 53956329 | ID=cds.MS.gene050253.t1;Parent=MS.gene050253.t1 |
| Gene Sequence |
| Protein sequence |
