Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26171.t1 | XP_003616173.1 | 97.6 | 370 | 9 | 0 | 1 | 370 | 1 | 370 | 2.10E-209 | 738 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26171.t1 | G7KFU4 | 97.6 | 370 | 9 | 0 | 1 | 370 | 1 | 370 | 1.5e-209 | 738.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050317 | MS.gene26171 | 0.80841 | 3.14E-50 | -1.69E-46 |
| MS.gene058639 | MS.gene26171 | 0.805401 | 1.36E-49 | -1.69E-46 |
| MS.gene060563 | MS.gene26171 | 0.800923 | 1.15E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26171.t1 | MTR_5g076930 | 97.568 | 370 | 9 | 0 | 1 | 370 | 1 | 370 | 0.0 | 741 |
| MS.gene26171.t1 | MTR_5g076940 | 81.481 | 108 | 18 | 1 | 265 | 370 | 1 | 108 | 1.99e-55 | 177 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26171.t1 | AT3G03210 | 57.713 | 376 | 145 | 6 | 1 | 370 | 1 | 368 | 6.30e-140 | 402 |
Find 67 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTACAAATGCTTCTGATTA+TGG | 0.165491 | 5.4:-57468610 | MS.gene26171:CDS |
| CTTTGCTTCATGAGATTGTT+TGG | 0.216592 | 5.4:-57469059 | MS.gene26171:CDS |
| TTCTTTGTTGAGTTTAGTTT+TGG | 0.229067 | 5.4:-57468839 | MS.gene26171:CDS |
| TGATGAGATGGGTTTGAAAT+TGG | 0.236912 | 5.4:-57468749 | MS.gene26171:CDS |
| TCCTAACAATTTGACAGATT+TGG | 0.259155 | 5.4:-57468707 | MS.gene26171:CDS |
| GGTTCATCATTCTCAAATTT+CGG | 0.287793 | 5.4:+57468535 | None:intergenic |
| TGGATTTGCTTAATGGGTCT+TGG | 0.290048 | 5.4:-57468903 | MS.gene26171:CDS |
| TTAGATCGCCGCATTTGTTT+TGG | 0.290342 | 5.4:-57468489 | MS.gene26171:CDS |
| CATTGAATTATGATAAGTTT+TGG | 0.296461 | 5.4:-57468990 | MS.gene26171:CDS |
| ACATTTGTATCCGGATGTTT+TGG | 0.314178 | 5.4:-57468665 | MS.gene26171:CDS |
| TCAGAAACAGAAGGAAAATA+AGG | 0.316073 | 5.4:+57469150 | None:intergenic |
| TCTGATTATGGTGACTTGTT+GGG | 0.319697 | 5.4:-57468598 | MS.gene26171:CDS |
| TTCTCAAATTTCGGCGAAAC+AGG | 0.321721 | 5.4:+57468544 | None:intergenic |
| GCTTAAGAGGAAGGATGTTA+TGG | 0.329703 | 5.4:-57468923 | MS.gene26171:CDS |
| GATGTTATGGATTTGCTTAA+TGG | 0.332464 | 5.4:-57468910 | MS.gene26171:CDS |
| CCATTTCAACTACTCGATAT+AGG | 0.351255 | 5.4:-57468337 | MS.gene26171:CDS |
| GGATTTGCTTAATGGGTCTT+GGG | 0.351297 | 5.4:-57468902 | MS.gene26171:CDS |
| ATGTTATGGATTTGCTTAAT+GGG | 0.354857 | 5.4:-57468909 | MS.gene26171:CDS |
| TTTGCTTCATGAGATTGTTT+GGG | 0.355412 | 5.4:-57469058 | MS.gene26171:CDS |
| TTCTGATTATGGTGACTTGT+TGG | 0.369091 | 5.4:-57468599 | MS.gene26171:CDS |
| GTGAGTTTCAGAGGCTTAAG+AGG | 0.380831 | 5.4:-57468936 | MS.gene26171:CDS |
| AATGTTTGGTGCTGTTCAAT+TGG | 0.384156 | 5.4:-57469301 | MS.gene26171:CDS |
| ACCAAATCTGTCAAATTGTT+AGG | 0.393608 | 5.4:+57468706 | None:intergenic |
| TGTTGTATTGTTTGTTCCAA+TGG | 0.395106 | 5.4:-57469262 | MS.gene26171:CDS |
| TGGTGATGGGTTCTGGTTTG+TGG | 0.402650 | 5.4:-57468645 | MS.gene26171:CDS |
| GGGTCTTGGGTTATGATTGT+AGG | 0.403798 | 5.4:-57468889 | MS.gene26171:CDS |
| ATGAATTATTTCTCAACAAA+GGG | 0.415974 | 5.4:+57469010 | None:intergenic |
| TAAGAAGAAACATTTGTATC+CGG | 0.417131 | 5.4:-57468674 | MS.gene26171:CDS |
| GGATTCGGAGCGGGTTGAAT+CGG | 0.419839 | 5.4:-57468818 | MS.gene26171:CDS |
| CCTATATCGAGTAGTTGAAA+TGG | 0.422886 | 5.4:+57468337 | None:intergenic |
| CATATTGATATTGATGAGAT+GGG | 0.430367 | 5.4:-57468760 | MS.gene26171:CDS |
| TCTGTCAAATTGTTAGGATA+AGG | 0.433218 | 5.4:+57468712 | None:intergenic |
| AAATGATGATGTTGTGTTTG+TGG | 0.434990 | 5.4:-57469103 | MS.gene26171:CDS |
| TCATATTGATATTGATGAGA+TGG | 0.436718 | 5.4:-57468761 | MS.gene26171:CDS |
| AACATAGAGTTTATCAAAGT+CGG | 0.442553 | 5.4:+57468457 | None:intergenic |
| GTTGTATTGTTTGTTCCAAT+GGG | 0.452111 | 5.4:-57469261 | MS.gene26171:CDS |
| TTGGGAGGTTATGCCAAGTA+AGG | 0.454727 | 5.4:-57469040 | MS.gene26171:CDS |
| CTGATTATGGTGACTTGTTG+GGG | 0.455040 | 5.4:-57468597 | MS.gene26171:CDS |
| TGCGATGGGTTCTGTTTCAT+CGG | 0.467835 | 5.4:-57468512 | MS.gene26171:CDS |
| TTTGTTCCAATGGGTATGGC+TGG | 0.472651 | 5.4:-57469252 | MS.gene26171:CDS |
| AATGAATTATTTCTCAACAA+AGG | 0.477205 | 5.4:+57469009 | None:intergenic |
| ATTGTTTGTTCCAATGGGTA+TGG | 0.477514 | 5.4:-57469256 | MS.gene26171:CDS |
| GGTCTTGGGTTATGATTGTA+GGG | 0.490359 | 5.4:-57468888 | MS.gene26171:CDS |
| ATGTTTGGTGCTGTTCAATT+GGG | 0.492915 | 5.4:-57469300 | MS.gene26171:CDS |
| AGTGAAGATGAATGATGTAA+TGG | 0.493702 | 5.4:-57468416 | MS.gene26171:CDS |
| TTCCAATGGGTATGGCTGGT+TGG | 0.506316 | 5.4:-57469248 | MS.gene26171:CDS |
| GCTTCATGAGATTGTTTGGG+AGG | 0.519554 | 5.4:-57469055 | MS.gene26171:CDS |
| TGAAGAATAAAACTTTGTTA+CGG | 0.526592 | 5.4:+57469217 | None:intergenic |
| CGGAGCGGGTTGAATCGGTT+AGG | 0.533530 | 5.4:-57468813 | MS.gene26171:CDS |
| TGGAGTAGATTGAATTCTGT+TGG | 0.534685 | 5.4:-57468964 | MS.gene26171:CDS |
| TTGGTGAATGTGAGTTTCAG+AGG | 0.541342 | 5.4:-57468945 | MS.gene26171:CDS |
| GAACCCATCACCAAAACATC+CGG | 0.544298 | 5.4:+57468655 | None:intergenic |
| GTTTCAGAGGCTTAAGAGGA+AGG | 0.545889 | 5.4:-57468932 | MS.gene26171:CDS |
| GGATTATTGAGTTTGAATTG+TGG | 0.565016 | 5.4:-57468316 | MS.gene26171:CDS |
| TGCCAACCAGCCATACCCAT+TGG | 0.570463 | 5.4:+57469246 | None:intergenic |
| TGAGAATGATGAACCTGCGA+TGG | 0.581367 | 5.4:-57468527 | MS.gene26171:CDS |
| TTGTAGGGGATTCACAAGCT+AGG | 0.585033 | 5.4:-57468873 | MS.gene26171:CDS |
| GGAATCAAATGTACAGATGA+TGG | 0.585892 | 5.4:-57468295 | MS.gene26171:CDS |
| GATGATGGTATGCATTATGA+TGG | 0.601643 | 5.4:-57468280 | MS.gene26171:CDS |
| GTCTTGGGTTATGATTGTAG+GGG | 0.606642 | 5.4:-57468887 | MS.gene26171:CDS |
| GAGAATGATGAACCTGCGAT+GGG | 0.610564 | 5.4:-57468526 | MS.gene26171:CDS |
| CTCATAAAATCAGAAACAGA+AGG | 0.611262 | 5.4:+57469141 | None:intergenic |
| AAACTCTATGTTGAATACGC+AGG | 0.625290 | 5.4:-57468446 | MS.gene26171:CDS |
| GTTGTGTTTGTGGAAGATCG+TGG | 0.630808 | 5.4:-57469093 | MS.gene26171:CDS |
| GATGAAACAGAACCCATCGC+AGG | 0.633804 | 5.4:+57468514 | None:intergenic |
| AAGATGAATGATGTAATGGA+AGG | 0.671697 | 5.4:-57468412 | MS.gene26171:CDS |
| ATACCTAGCCAAAACAAATG+CGG | 0.712684 | 5.4:+57468481 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTGAAATTGGATTTTATTT+GGG | - | chr5.4:57468773-57468792 | MS.gene26171:CDS | 15.0% |
| !! | TGAAGAATAAAACTTTGTTA+CGG | + | chr5.4:57468296-57468315 | None:intergenic | 20.0% |
| !!! | AATGAATTATTTCTCAACAA+AGG | + | chr5.4:57468504-57468523 | None:intergenic | 20.0% |
| !!! | ATGAATTATTTCTCAACAAA+GGG | + | chr5.4:57468503-57468522 | None:intergenic | 20.0% |
| !!! | CATTGAATTATGATAAGTTT+TGG | - | chr5.4:57468520-57468539 | MS.gene26171:CDS | 20.0% |
| !!! | GTTTGAAATTGGATTTTATT+TGG | - | chr5.4:57468772-57468791 | MS.gene26171:CDS | 20.0% |
| ! | AACATAGAGTTTATCAAAGT+CGG | + | chr5.4:57469056-57469075 | None:intergenic | 25.0% |
| ! | ATTACAAATGCTTCTGATTA+TGG | - | chr5.4:57468900-57468919 | MS.gene26171:CDS | 25.0% |
| ! | CATATTGATATTGATGAGAT+GGG | - | chr5.4:57468750-57468769 | MS.gene26171:CDS | 25.0% |
| ! | TAAGAAGAAACATTTGTATC+CGG | - | chr5.4:57468836-57468855 | MS.gene26171:CDS | 25.0% |
| ! | TCATATTGATATTGATGAGA+TGG | - | chr5.4:57468749-57468768 | MS.gene26171:CDS | 25.0% |
| !! | ATGTTATGGATTTGCTTAAT+GGG | - | chr5.4:57468601-57468620 | MS.gene26171:CDS | 25.0% |
| !!! | AACAAAGTTTTATTCTTCAG+TGG | - | chr5.4:57468297-57468316 | MS.gene26171:CDS | 25.0% |
| !!! | ATTATGATAAGTTTTGGTCT+TGG | - | chr5.4:57468526-57468545 | MS.gene26171:CDS | 25.0% |
| !!! | TTAGGAGTTTTTTGTTTAAG+AGG | - | chr5.4:57468715-57468734 | MS.gene26171:CDS | 25.0% |
| !!! | TTCTTTGTTGAGTTTAGTTT+TGG | - | chr5.4:57468671-57468690 | MS.gene26171:CDS | 25.0% |
| ACCAAATCTGTCAAATTGTT+AGG | + | chr5.4:57468807-57468826 | None:intergenic | 30.0% | |
| AGTGAAGATGAATGATGTAA+TGG | - | chr5.4:57469094-57469113 | MS.gene26171:CDS | 30.0% | |
| CTCATAAAATCAGAAACAGA+AGG | + | chr5.4:57468372-57468391 | None:intergenic | 30.0% | |
| GGATTATTGAGTTTGAATTG+TGG | - | chr5.4:57469194-57469213 | MS.gene26171:CDS | 30.0% | |
| GGTTCATCATTCTCAAATTT+CGG | + | chr5.4:57468978-57468997 | None:intergenic | 30.0% | |
| GTTGTATTGTTTGTTCCAAT+GGG | - | chr5.4:57468249-57468268 | MS.gene26171:CDS | 30.0% | |
| TCAGAAACAGAAGGAAAATA+AGG | + | chr5.4:57468363-57468382 | None:intergenic | 30.0% | |
| TCCTAACAATTTGACAGATT+TGG | - | chr5.4:57468803-57468822 | MS.gene26171:CDS | 30.0% | |
| TCTGTCAAATTGTTAGGATA+AGG | + | chr5.4:57468801-57468820 | None:intergenic | 30.0% | |
| TGTTGTATTGTTTGTTCCAA+TGG | - | chr5.4:57468248-57468267 | MS.gene26171:CDS | 30.0% | |
| TTTGCTTCATGAGATTGTTT+GGG | - | chr5.4:57468452-57468471 | MS.gene26171:CDS | 30.0% | |
| ! | AAATGATGATGTTGTGTTTG+TGG | - | chr5.4:57468407-57468426 | MS.gene26171:CDS | 30.0% |
| ! | AAGATGAATGATGTAATGGA+AGG | - | chr5.4:57469098-57469117 | MS.gene26171:CDS | 30.0% |
| ! | GATGTTATGGATTTGCTTAA+TGG | - | chr5.4:57468600-57468619 | MS.gene26171:CDS | 30.0% |
| ! | TTCGAAAAAGTGGTATTTTG+AGG | - | chr5.4:57469138-57469157 | MS.gene26171:CDS | 30.0% |
| !! | TCGAAAAAGTGGTATTTTGA+GGG | - | chr5.4:57469139-57469158 | MS.gene26171:CDS | 30.0% |
| !!! | GTTGAGTTTAGTTTTGGATT+CGG | - | chr5.4:57468677-57468696 | MS.gene26171:CDS | 30.0% |
| AAACTCTATGTTGAATACGC+AGG | - | chr5.4:57469064-57469083 | MS.gene26171:CDS | 35.0% | |
| AAAGGGTCAAAAACCTTACT+TGG | + | chr5.4:57468486-57468505 | None:intergenic | 35.0% | |
| ATACCTAGCCAAAACAAATG+CGG | + | chr5.4:57469032-57469051 | None:intergenic | 35.0% | |
| ATTGTTTGTTCCAATGGGTA+TGG | - | chr5.4:57468254-57468273 | MS.gene26171:CDS | 35.0% | |
| CCATTTCAACTACTCGATAT+AGG | - | chr5.4:57469173-57469192 | MS.gene26171:CDS | 35.0% | |
| CCTATATCGAGTAGTTGAAA+TGG | + | chr5.4:57469176-57469195 | None:intergenic | 35.0% | |
| CTTTGCTTCATGAGATTGTT+TGG | - | chr5.4:57468451-57468470 | MS.gene26171:CDS | 35.0% | |
| GGAATCAAATGTACAGATGA+TGG | - | chr5.4:57469215-57469234 | MS.gene26171:CDS | 35.0% | |
| TGGAGTAGATTGAATTCTGT+TGG | - | chr5.4:57468546-57468565 | MS.gene26171:CDS | 35.0% | |
| ! | ACATTTGTATCCGGATGTTT+TGG | - | chr5.4:57468845-57468864 | MS.gene26171:CDS | 35.0% |
| ! | CTTTTCATTACTCTTGCAGT+TGG | - | chr5.4:57468324-57468343 | MS.gene26171:CDS | 35.0% |
| ! | GATGATGGTATGCATTATGA+TGG | - | chr5.4:57469230-57469249 | MS.gene26171:CDS | 35.0% |
| ! | TGATGAGATGGGTTTGAAAT+TGG | - | chr5.4:57468761-57468780 | MS.gene26171:CDS | 35.0% |
| ! | TTCTGATTATGGTGACTTGT+TGG | - | chr5.4:57468911-57468930 | MS.gene26171:CDS | 35.0% |
| !! | ATGTTTGGTGCTGTTCAATT+GGG | - | chr5.4:57468210-57468229 | MS.gene26171:CDS | 35.0% |
| !! | TCTGATTATGGTGACTTGTT+GGG | - | chr5.4:57468912-57468931 | MS.gene26171:CDS | 35.0% |
| GCTTAAGAGGAAGGATGTTA+TGG | - | chr5.4:57468587-57468606 | MS.gene26171:CDS | 40.0% | |
| GGTCTTGGGTTATGATTGTA+GGG | - | chr5.4:57468622-57468641 | MS.gene26171:CDS | 40.0% | |
| GTCTTGGGTTATGATTGTAG+GGG | - | chr5.4:57468623-57468642 | MS.gene26171:CDS | 40.0% | |
| TTCTCAAATTTCGGCGAAAC+AGG | + | chr5.4:57468969-57468988 | None:intergenic | 40.0% | |
| TTGGTGAATGTGAGTTTCAG+AGG | - | chr5.4:57468565-57468584 | MS.gene26171:CDS | 40.0% | |
| ! | GGATTTGCTTAATGGGTCTT+GGG | - | chr5.4:57468608-57468627 | MS.gene26171:CDS | 40.0% |
| ! | TGGATTTGCTTAATGGGTCT+TGG | - | chr5.4:57468607-57468626 | MS.gene26171:CDS | 40.0% |
| ! | TTAGATCGCCGCATTTGTTT+TGG | - | chr5.4:57469021-57469040 | MS.gene26171:CDS | 40.0% |
| !! | AGTGGTATTTTGAGGGAGTT+TGG | - | chr5.4:57469146-57469165 | MS.gene26171:CDS | 40.0% |
| !! | CTGATTATGGTGACTTGTTG+GGG | - | chr5.4:57468913-57468932 | MS.gene26171:CDS | 40.0% |
| !!! | GTTTAGTTTTGGATTCGGAG+CGG | - | chr5.4:57468682-57468701 | MS.gene26171:CDS | 40.0% |
| !!! | TATCCGGATGTTTTGGTGAT+GGG | - | chr5.4:57468852-57468871 | MS.gene26171:CDS | 40.0% |
| !!! | TTTAGTTTTGGATTCGGAGC+GGG | - | chr5.4:57468683-57468702 | MS.gene26171:CDS | 40.0% |
| GAACCCATCACCAAAACATC+CGG | + | chr5.4:57468858-57468877 | None:intergenic | 45.0% | |
| GAGAATGATGAACCTGCGAT+GGG | - | chr5.4:57468984-57469003 | MS.gene26171:CDS | 45.0% | |
| GAGAGAGAGCTTCGAAAAAG+TGG | - | chr5.4:57469128-57469147 | MS.gene26171:CDS | 45.0% | |
| GCTTCATGAGATTGTTTGGG+AGG | - | chr5.4:57468455-57468474 | MS.gene26171:CDS | 45.0% | |
| GGGTCTTGGGTTATGATTGT+AGG | - | chr5.4:57468621-57468640 | MS.gene26171:CDS | 45.0% | |
| GTGAGTTTCAGAGGCTTAAG+AGG | - | chr5.4:57468574-57468593 | MS.gene26171:CDS | 45.0% | |
| GTTGTGTTTGTGGAAGATCG+TGG | - | chr5.4:57468417-57468436 | MS.gene26171:CDS | 45.0% | |
| GTTTCAGAGGCTTAAGAGGA+AGG | - | chr5.4:57468578-57468597 | MS.gene26171:CDS | 45.0% | |
| TGAGAATGATGAACCTGCGA+TGG | - | chr5.4:57468983-57469002 | MS.gene26171:CDS | 45.0% | |
| TTGGGAGGTTATGCCAAGTA+AGG | - | chr5.4:57468470-57468489 | MS.gene26171:CDS | 45.0% | |
| TTGTAGGGGATTCACAAGCT+AGG | - | chr5.4:57468637-57468656 | MS.gene26171:CDS | 45.0% | |
| TTTGTTCCAATGGGTATGGC+TGG | - | chr5.4:57468258-57468277 | MS.gene26171:CDS | 45.0% | |
| ! | TGCGATGGGTTCTGTTTCAT+CGG | - | chr5.4:57468998-57469017 | MS.gene26171:CDS | 45.0% |
| !! | GGTATTTTGAGGGAGTTTGG+TGG | - | chr5.4:57469149-57469168 | MS.gene26171:CDS | 45.0% |
| !!! | GATGTTTTGGTGATGGGTTC+TGG | - | chr5.4:57468858-57468877 | MS.gene26171:CDS | 45.0% |
| !!! | GTATCCGGATGTTTTGGTGA+TGG | - | chr5.4:57468851-57468870 | MS.gene26171:CDS | 45.0% |
| GATGAAACAGAACCCATCGC+AGG | + | chr5.4:57468999-57469018 | None:intergenic | 50.0% | |
| TTCCAATGGGTATGGCTGGT+TGG | - | chr5.4:57468262-57468281 | MS.gene26171:CDS | 50.0% | |
| !! | TGGTGATGGGTTCTGGTTTG+TGG | - | chr5.4:57468865-57468884 | MS.gene26171:CDS | 50.0% |
| !!! | TCGCCGCATTTGTTTTGGCT+AGG | - | chr5.4:57469026-57469045 | MS.gene26171:CDS | 50.0% |
| TGCCAACCAGCCATACCCAT+TGG | + | chr5.4:57468267-57468286 | None:intergenic | 55.0% | |
| !! | GGATTCGGAGCGGGTTGAAT+CGG | - | chr5.4:57468692-57468711 | MS.gene26171:CDS | 55.0% |
| !! | CGGAGCGGGTTGAATCGGTT+AGG | - | chr5.4:57468697-57468716 | MS.gene26171:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 57468210 | 57469322 | 57468210 | ID=MS.gene26171 |
| chr5.4 | mRNA | 57468210 | 57469322 | 57468210 | ID=MS.gene26171.t1;Parent=MS.gene26171 |
| chr5.4 | exon | 57468210 | 57469322 | 57468210 | ID=MS.gene26171.t1.exon1;Parent=MS.gene26171.t1 |
| chr5.4 | CDS | 57468210 | 57469322 | 57468210 | ID=cds.MS.gene26171.t1;Parent=MS.gene26171.t1 |
| Gene Sequence |
| Protein sequence |