AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050317


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050317.t1 MTR_7g104610 98.039 153 3 0 1 153 1 153 1.93e-107 303
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050317.t1 AT5G15770 71.918 146 41 0 7 152 3 148 3.14e-74 219

Find 27 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCGTTGCAATAATCTTTC+CGG 0.199227 7.2:+8266717 None:intergenic
GTCTGTAATCTCTAATCTTC+TGG 0.252175 7.2:+8266879 None:intergenic
TCGTTCTGTTGGGTGTTATA+AGG 0.300594 7.2:-8266563 MS.gene050317:CDS
TAAGAAATTGTGGTAAAGTT+GGG 0.348212 7.2:-8266669 MS.gene050317:CDS
TTAAGAAATTGTGGTAAAGT+TGG 0.366943 7.2:-8266670 MS.gene050317:CDS
ACCGATCATGCTCGTTCTGT+TGG 0.371791 7.2:-8266574 MS.gene050317:CDS
AGTGCTCGTGGGAAACAATT+GGG 0.376890 7.2:-8266619 MS.gene050317:CDS
ACCGGAAAGATTATTGCAAC+GGG 0.406061 7.2:-8266718 MS.gene050317:CDS
GTTATTGAAGATGAGATTAC+CGG 0.417687 7.2:-8266736 MS.gene050317:CDS
AAGTGCTCGTGGGAAACAAT+TGG 0.427736 7.2:-8266620 MS.gene050317:CDS
CCGATCATGCTCGTTCTGTT+GGG 0.443895 7.2:-8266573 MS.gene050317:CDS
AGTCTCTGATAAGGAATTTG+AGG 0.451854 7.2:-8266806 MS.gene050317:CDS
TTGTGATTCAGTCTCTGATA+AGG 0.468883 7.2:-8266815 MS.gene050317:CDS
TTTCGAGAGGTTGATTCACT+TGG 0.471380 7.2:-8266778 MS.gene050317:CDS
CGATTGCAGTGTTGAGAACA+AGG 0.494534 7.2:-8266533 MS.gene050317:CDS
CTATGAGAAATGTGGTTTCA+AGG 0.496011 7.2:-8266506 MS.gene050317:CDS
CCCAACAGAACGAGCATGAT+CGG 0.532044 7.2:+8266573 None:intergenic
TACCGGAAAGATTATTGCAA+CGG 0.546187 7.2:-8266719 MS.gene050317:CDS
CAAGGAGAAAGAAGTTCAGA+TGG 0.566779 7.2:-8266488 MS.gene050317:CDS
GTTGTGGATTCAAGTGCTCG+TGG 0.610212 7.2:-8266631 MS.gene050317:CDS
ATTTGAGGATCGATTTCGAG+AGG 0.616403 7.2:-8266791 MS.gene050317:CDS
GCATATAGAAGATGTTGTTG+TGG 0.621571 7.2:-8266647 MS.gene050317:CDS
GTGCTCGTGGGAAACAATTG+GGG 0.624260 7.2:-8266618 MS.gene050317:CDS
TTGTGGATTCAAGTGCTCGT+GGG 0.642750 7.2:-8266630 MS.gene050317:CDS
AAGGTGTTCTATGAGAAATG+TGG 0.671486 7.2:-8266514 MS.gene050317:CDS
TTAGAAGATGCAAAACAGTG+AGG 0.719583 7.2:-8266917 None:intergenic
CCGGAAAGATTATTGCAACG+GGG 0.727047 7.2:-8266717 MS.gene050317:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAAATTTTTAAGAAATTG+TGG - chr7.2:8266702-8266721 MS.gene050317:CDS 15.0%
! TAAGAAATTGTGGTAAAGTT+GGG - chr7.2:8266712-8266731 MS.gene050317:CDS 25.0%
! TTAAGAAATTGTGGTAAAGT+TGG - chr7.2:8266711-8266730 MS.gene050317:CDS 25.0%
GAGATTACAGACAAGAAAAA+AGG - chr7.2:8266513-8266532 MS.gene050317:CDS 30.0%
GTTATTGAAGATGAGATTAC+CGG - chr7.2:8266645-8266664 MS.gene050317:CDS 30.0%
AGTCTCTGATAAGGAATTTG+AGG - chr7.2:8266575-8266594 MS.gene050317:CDS 35.0%
CTATGAGAAATGTGGTTTCA+AGG - chr7.2:8266875-8266894 MS.gene050317:CDS 35.0%
GCATATAGAAGATGTTGTTG+TGG - chr7.2:8266734-8266753 MS.gene050317:CDS 35.0%
GTCTGTAATCTCTAATCTTC+TGG + chr7.2:8266505-8266524 None:intergenic 35.0%
TACCGGAAAGATTATTGCAA+CGG - chr7.2:8266662-8266681 MS.gene050317:CDS 35.0%
TTGTGATTCAGTCTCTGATA+AGG - chr7.2:8266566-8266585 MS.gene050317:CDS 35.0%
! AAGGTGTTCTATGAGAAATG+TGG - chr7.2:8266867-8266886 MS.gene050317:CDS 35.0%
ACCGGAAAGATTATTGCAAC+GGG - chr7.2:8266663-8266682 MS.gene050317:CDS 40.0%
CAAGGAGAAAGAAGTTCAGA+TGG - chr7.2:8266893-8266912 MS.gene050317:CDS 40.0%
! ATTTGAGGATCGATTTCGAG+AGG - chr7.2:8266590-8266609 MS.gene050317:CDS 40.0%
! TCGTTCTGTTGGGTGTTATA+AGG - chr7.2:8266818-8266837 MS.gene050317:CDS 40.0%
! TTTCGAGAGGTTGATTCACT+TGG - chr7.2:8266603-8266622 MS.gene050317:CDS 40.0%
AAGTGCTCGTGGGAAACAAT+TGG - chr7.2:8266761-8266780 MS.gene050317:CDS 45.0%
AGTGCTCGTGGGAAACAATT+GGG - chr7.2:8266762-8266781 MS.gene050317:CDS 45.0%
CCCCGTTGCAATAATCTTTC+CGG + chr7.2:8266667-8266686 None:intergenic 45.0%
CCGGAAAGATTATTGCAACG+GGG - chr7.2:8266664-8266683 MS.gene050317:CDS 45.0%
TTGTGGATTCAAGTGCTCGT+GGG - chr7.2:8266751-8266770 MS.gene050317:CDS 45.0%
! CGATTGCAGTGTTGAGAACA+AGG - chr7.2:8266848-8266867 MS.gene050317:CDS 45.0%
ACCGATCATGCTCGTTCTGT+TGG - chr7.2:8266807-8266826 MS.gene050317:CDS 50.0%
CCCAACAGAACGAGCATGAT+CGG + chr7.2:8266811-8266830 None:intergenic 50.0%
CCGATCATGCTCGTTCTGTT+GGG - chr7.2:8266808-8266827 MS.gene050317:CDS 50.0%
GTGCTCGTGGGAAACAATTG+GGG - chr7.2:8266763-8266782 MS.gene050317:CDS 50.0%
GTTGTGGATTCAAGTGCTCG+TGG - chr7.2:8266750-8266769 MS.gene050317:CDS 50.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 8266471 8266932 8266471 ID=MS.gene050317
chr7.2 mRNA 8266471 8266932 8266471 ID=MS.gene050317.t1;Parent=MS.gene050317
chr7.2 exon 8266471 8266932 8266471 ID=MS.gene050317.t1.exon1;Parent=MS.gene050317.t1
chr7.2 CDS 8266471 8266932 8266471 ID=cds.MS.gene050317.t1;Parent=MS.gene050317.t1
Gene Sequence

>MS.gene050317

ATGCAAAACAGTGAGGAACAAAAGTATAGAGCCAGAAGATTAGAGATTACAGACAAGAAAAAAGGTTTCATAGAATTACTACAACAGCTTAGCGTTTGTGATTCAGTCTCTGATAAGGAATTTGAGGATCGATTTCGAGAGGTTGATTCACTTGGAGATGATCATGTTATATGTGTTATTGAAGATGAGATTACCGGAAAGATTATTGCAACGGGGAGTGTGTTTATTGAGAAGAAATTTTTAAGAAATTGTGGTAAAGTTGGGCATATAGAAGATGTTGTTGTGGATTCAAGTGCTCGTGGGAAACAATTGGGGAAGAAAGTAATTAACTTTCTTACCGATCATGCTCGTTCTGTTGGGTGTTATAAGGTAATTCTCGATTGCAGTGTTGAGAACAAGGTGTTCTATGAGAAATGTGGTTTCAAGGAGAAAGAAGTTCAGATGGCTATGTATTTTGTTTGA

Protein sequence

>MS.gene050317.t1

MQNSEEQKYRARRLEITDKKKGFIELLQQLSVCDSVSDKEFEDRFREVDSLGDDHVICVIEDEITGKIIATGSVFIEKKFLRNCGKVGHIEDVVVDSSARGKQLGKKVINFLTDHARSVGCYKVILDCSVENKVFYEKCGFKEKEVQMAMYFV