Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26677.t1 | XP_003609992.1 | 97.9 | 190 | 4 | 0 | 1 | 190 | 1 | 190 | 1.90E-105 | 391.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26677.t1 | G7JT65 | 97.9 | 190 | 4 | 0 | 1 | 190 | 1 | 190 | 1.4e-105 | 391.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049190 | MS.gene26677 | 0.834567 | 2.78E-56 | -1.69E-46 |
| MS.gene053144 | MS.gene26677 | 0.832082 | 1.16E-55 | -1.69E-46 |
| MS.gene054584 | MS.gene26677 | 0.827758 | 1.31E-54 | -1.69E-46 |
| MS.gene05638 | MS.gene26677 | 0.804792 | 1.82E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26677.t1 | MTR_4g125100 | 97.895 | 190 | 4 | 0 | 1 | 190 | 1 | 190 | 4.06e-139 | 385 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26677.t1 | AT1G19130 | 68.254 | 189 | 57 | 3 | 3 | 190 | 6 | 192 | 4.42e-90 | 262 |
Find 39 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGTTAAATTTACATGTCT+TGG | 0.348435 | 8.4:-9653590 | MS.gene26677:CDS |
| TCCACGTAAAATCCACCTTC+TGG | 0.366637 | 8.4:+9654565 | None:intergenic |
| GAAACTCATTACTCTCTTGT+AGG | 0.414889 | 8.4:-9653434 | MS.gene26677:CDS |
| ATATACTTTCTGCTTCCATC+TGG | 0.431150 | 8.4:-9654381 | MS.gene26677:CDS |
| ACACGGTGCCTCCGAATGTC+TGG | 0.437212 | 8.4:-9653535 | MS.gene26677:CDS |
| GTAGGATGCACTTGTGCACC+TGG | 0.437438 | 8.4:-9653416 | MS.gene26677:CDS |
| GTGCCTCCGAATGTCTGGTT+TGG | 0.451216 | 8.4:-9653530 | MS.gene26677:CDS |
| AACAAAGGAATTACGGTGAT+AGG | 0.454551 | 8.4:+9654297 | None:intergenic |
| GTTTAGCAAGTTCAAAATCC+TGG | 0.459347 | 8.4:+9653384 | None:intergenic |
| ATCAGAAAGAATGTCTTTGT+CGG | 0.473073 | 8.4:-9654626 | None:intergenic |
| TGATGCCATGTTTCAGAAGA+AGG | 0.497508 | 8.4:+9654333 | None:intergenic |
| AAAGAACCAAACCAGACATT+CGG | 0.516467 | 8.4:+9653524 | None:intergenic |
| GAAACATGGCATCATTACAT+TGG | 0.519533 | 8.4:-9654324 | MS.gene26677:CDS |
| AAGAATGTCTTTGTCGGCAT+CGG | 0.520028 | 8.4:-9654620 | None:intergenic |
| TCTTGGATCTGATCTAACTA+AGG | 0.525817 | 8.4:-9653573 | MS.gene26677:CDS |
| CAGAAGAAGGAATATGATGA+AGG | 0.540545 | 8.4:+9654346 | None:intergenic |
| TAAAATCCACCTTCTGGATG+AGG | 0.547021 | 8.4:+9654571 | None:intergenic |
| TTTACTTACATTCAGGTGGA+AGG | 0.547646 | 8.4:+9654503 | None:intergenic |
| TTTGCTGTCTCTGCAGATGG+GGG | 0.555085 | 8.4:-9653488 | MS.gene26677:CDS |
| AAGTATATGCAGGTCGACAC+TGG | 0.558993 | 8.4:+9654396 | None:intergenic |
| ATATTCCTTCTTCTGAAACA+TGG | 0.566858 | 8.4:-9654338 | MS.gene26677:CDS |
| GTGACAAGAGACTCCAATTG+AGG | 0.568370 | 8.4:+9653338 | None:intergenic |
| TTGCTGTCTCTGCAGATGGG+GGG | 0.568555 | 8.4:-9653487 | MS.gene26677:CDS |
| TGCAGAGACAGCAAAATCAC+TGG | 0.576800 | 8.4:+9653496 | None:intergenic |
| ATTGAGGAAAACGTGAAATG+AGG | 0.583631 | 8.4:+9653354 | None:intergenic |
| TGGAAGCAGAAAGTATATGC+AGG | 0.591339 | 8.4:+9654386 | None:intergenic |
| TTCGGAGGCACCGTGTATTG+CGG | 0.594043 | 8.4:+9653542 | None:intergenic |
| AGCGAGCAGCAACATTACTC+AGG | 0.596950 | 8.4:+9653302 | None:intergenic |
| CTGCAACCTCATCCAGAAGG+TGG | 0.598332 | 8.4:-9654577 | MS.gene26677:CDS |
| GTGAAGGGTTCAGAAAGAGA+TGG | 0.604440 | 8.4:-9653458 | MS.gene26677:CDS |
| AAATCCTGGAACTGAAAACC+AGG | 0.610826 | 8.4:+9653398 | None:intergenic |
| GGATCAGACACCGCAATACA+CGG | 0.615581 | 8.4:-9653552 | MS.gene26677:CDS |
| AGGCGAGACATGGTACCAGA+TGG | 0.617797 | 8.4:+9654366 | None:intergenic |
| AATATGATGAAGGCGAGACA+TGG | 0.618034 | 8.4:+9654356 | None:intergenic |
| GAGACAGCAAAATCACTGGT+CGG | 0.647906 | 8.4:+9653500 | None:intergenic |
| AATCTGCAACCTCATCCAGA+AGG | 0.653089 | 8.4:-9654580 | MS.gene26677:CDS |
| ATATGCAGGTCGACACTGGT+CGG | 0.655426 | 8.4:+9654400 | None:intergenic |
| AAGTAGAAAGTTGAACAGAG+TGG | 0.677760 | 8.4:+9654530 | None:intergenic |
| GAACCAAACCAGACATTCGG+AGG | 0.677908 | 8.4:+9653527 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAATATGATTGATAAATA+AGG | + | chr8.4:9653888-9653907 | None:intergenic | 10.0% |
| !! | AAAAAAAAACAAAGGAATTA+CGG | + | chr8.4:9653645-9653664 | None:intergenic | 15.0% |
| !! | TTTGTTAGTATAATAATACT+TGG | - | chr8.4:9654275-9654294 | MS.gene26677:intron | 15.0% |
| !! | AGATTGTTAATTCAGTATAT+GGG | - | chr8.4:9653736-9653755 | MS.gene26677:intron | 20.0% |
| !! | TAGATTGTTAATTCAGTATA+TGG | - | chr8.4:9653735-9653754 | MS.gene26677:intron | 20.0% |
| !! | TGAAAACGAAAAAAAAACAA+AGG | + | chr8.4:9653653-9653672 | None:intergenic | 20.0% |
| !!! | ATTTGATTTTACTTACATTC+AGG | + | chr8.4:9653439-9653458 | None:intergenic | 20.0% |
| !!! | CATTAGATTTGAAACATATT+GGG | - | chr8.4:9653984-9654003 | MS.gene26677:intron | 20.0% |
| !!! | TCATTAGATTTGAAACATAT+TGG | - | chr8.4:9653983-9654002 | MS.gene26677:intron | 20.0% |
| ! | AGTGTTAAATTTACATGTCT+TGG | - | chr8.4:9654342-9654361 | MS.gene26677:CDS | 25.0% |
| ! | TGATGAAATTGAAAAGAAAG+AGG | + | chr8.4:9653468-9653487 | None:intergenic | 25.0% |
| !!! | GGGGTTTTGAAATTTAATTT+TGG | - | chr8.4:9654011-9654030 | MS.gene26677:intron | 25.0% |
| GTTGAATTAACCAAAAACTG+TGG | + | chr8.4:9654149-9654168 | None:intergenic | 30.0% | |
| TTTGCATGTGTTGGAAAATT+GGG | - | chr8.4:9654237-9654256 | MS.gene26677:intron | 30.0% | |
| TTTGCTCCTTAAATACTGAT+TGG | - | chr8.4:9653909-9653928 | MS.gene26677:intron | 30.0% | |
| ! | ATTTGAAACATATTGGGAGT+TGG | - | chr8.4:9653990-9654009 | MS.gene26677:intron | 30.0% |
| ! | TGATTTTACTTACATTCAGG+TGG | + | chr8.4:9653436-9653455 | None:intergenic | 30.0% |
| ! | TTGCTCCTTAAATACTGATT+GGG | - | chr8.4:9653910-9653929 | MS.gene26677:intron | 30.0% |
| ! | TTTGAAACATATTGGGAGTT+GGG | - | chr8.4:9653991-9654010 | MS.gene26677:intron | 30.0% |
| ! | TTTTGCATGTGTTGGAAAAT+TGG | - | chr8.4:9654236-9654255 | MS.gene26677:intron | 30.0% |
| !! | AATTTTGGCGAAAAGATTTG+AGG | - | chr8.4:9654026-9654045 | MS.gene26677:intron | 30.0% |
| !! | ATATTCCTTCTTCTGAAACA+TGG | - | chr8.4:9653594-9653613 | MS.gene26677:CDS | 30.0% |
| !!! | ATTTGTTTTATTGTAGGTGG+TGG | - | chr8.4:9654299-9654318 | MS.gene26677:intron | 30.0% |
| !!! | CTTGGCATTTGTTTTATTGT+AGG | - | chr8.4:9654293-9654312 | MS.gene26677:intron | 30.0% |
| AAAGAACCAAACCAGACATT+CGG | + | chr8.4:9654411-9654430 | None:intergenic | 35.0% | |
| AACAAAGGAATTACGGTGAT+AGG | + | chr8.4:9653638-9653657 | None:intergenic | 35.0% | |
| AAGTAGAAAGTTGAACAGAG+TGG | + | chr8.4:9653405-9653424 | None:intergenic | 35.0% | |
| AGCAATACACTTTGTAGCAA+CGG | + | chr8.4:9653813-9653832 | None:intergenic | 35.0% | |
| ATATACTTTCTGCTTCCATC+TGG | - | chr8.4:9653551-9653570 | MS.gene26677:CDS | 35.0% | |
| ATTGAGGAAAACGTGAAATG+AGG | + | chr8.4:9654581-9654600 | None:intergenic | 35.0% | |
| CAGAAGAAGGAATATGATGA+AGG | + | chr8.4:9653589-9653608 | None:intergenic | 35.0% | |
| CGAAAAGATTTGAGGAAACA+TGG | - | chr8.4:9654034-9654053 | MS.gene26677:intron | 35.0% | |
| GAAACATGGCATCATTACAT+TGG | - | chr8.4:9653608-9653627 | MS.gene26677:CDS | 35.0% | |
| GAAACTCATTACTCTCTTGT+AGG | - | chr8.4:9654498-9654517 | MS.gene26677:intron | 35.0% | |
| GGAACTGTATTAGACGATTT+CGG | + | chr8.4:9654060-9654079 | None:intergenic | 35.0% | |
| GTTCTTTAATGTGATCAACC+AGG | + | chr8.4:9653693-9653712 | None:intergenic | 35.0% | |
| GTTTAGCAAGTTCAAAATCC+TGG | + | chr8.4:9654551-9654570 | None:intergenic | 35.0% | |
| TCTTGGATCTGATCTAACTA+AGG | - | chr8.4:9654359-9654378 | MS.gene26677:CDS | 35.0% | |
| TTCCAACACATGCAAAATTG+TGG | + | chr8.4:9654233-9654252 | None:intergenic | 35.0% | |
| TTGAAACATATTGGGAGTTG+GGG | - | chr8.4:9653992-9654011 | MS.gene26677:intron | 35.0% | |
| TTTACTTACATTCAGGTGGA+AGG | + | chr8.4:9653432-9653451 | None:intergenic | 35.0% | |
| ! | CATTTCACGTTTTCCTCAAT+TGG | - | chr8.4:9654581-9654600 | MS.gene26677:CDS | 35.0% |
| !!! | GGCATTTGTTTTATTGTAGG+TGG | - | chr8.4:9654296-9654315 | MS.gene26677:intron | 35.0% |
| AAACTAACTCACTACGCAAG+AGG | + | chr8.4:9653836-9653855 | None:intergenic | 40.0% | |
| AAATCCTGGAACTGAAAACC+AGG | + | chr8.4:9654537-9654556 | None:intergenic | 40.0% | |
| AATATGATGAAGGCGAGACA+TGG | + | chr8.4:9653579-9653598 | None:intergenic | 40.0% | |
| ATCACATCTATAGCTGCATC+AGG | + | chr8.4:9654081-9654100 | None:intergenic | 40.0% | |
| CACCTCCCAATCAGTATTTA+AGG | + | chr8.4:9653918-9653937 | None:intergenic | 40.0% | |
| GATTGCAATAGTTGCGATTG+TGG | - | chr8.4:9653781-9653800 | MS.gene26677:intron | 40.0% | |
| TAAAATCCACCTTCTGGATG+AGG | + | chr8.4:9653364-9653383 | None:intergenic | 40.0% | |
| TGATGCCATGTTTCAGAAGA+AGG | + | chr8.4:9653602-9653621 | None:intergenic | 40.0% | |
| ! | AATTTTTGTCTTGCGACACC+TGG | - | chr8.4:9653672-9653691 | MS.gene26677:intron | 40.0% |
| ! | CTCCTTAAATACTGATTGGG+AGG | - | chr8.4:9653913-9653932 | MS.gene26677:intron | 40.0% |
| ! | GACCACAATTTTGCATGTGT+TGG | - | chr8.4:9654228-9654247 | MS.gene26677:intron | 40.0% |
| ! | TGGAAGCAGAAAGTATATGC+AGG | + | chr8.4:9653549-9653568 | None:intergenic | 40.0% |
| !! | ATTTTGCTGTCTCTGCAGAT+GGG | - | chr8.4:9654442-9654461 | MS.gene26677:intron | 40.0% |
| AAGTATATGCAGGTCGACAC+TGG | + | chr8.4:9653539-9653558 | None:intergenic | 45.0% | |
| AATCTGCAACCTCATCCAGA+AGG | - | chr8.4:9653352-9653371 | MS.gene26677:CDS | 45.0% | |
| GAGACAGCAAAATCACTGGT+CGG | + | chr8.4:9654435-9654454 | None:intergenic | 45.0% | |
| GATTGAAGATCACACTGCAG+TGG | - | chr8.4:9654098-9654117 | MS.gene26677:intron | 45.0% | |
| GTGAAGGGTTCAGAAAGAGA+TGG | - | chr8.4:9654474-9654493 | MS.gene26677:intron | 45.0% | |
| GTGACAAGAGACTCCAATTG+AGG | + | chr8.4:9654597-9654616 | None:intergenic | 45.0% | |
| TCCACGTAAAATCCACCTTC+TGG | + | chr8.4:9653370-9653389 | None:intergenic | 45.0% | |
| TGCAGAGACAGCAAAATCAC+TGG | + | chr8.4:9654439-9654458 | None:intergenic | 45.0% | |
| !! | GATTTTGCTGTCTCTGCAGA+TGG | - | chr8.4:9654441-9654460 | MS.gene26677:intron | 45.0% |
| !! | TCCAGAAGGTGGATTTTACG+TGG | - | chr8.4:9653366-9653385 | MS.gene26677:CDS | 45.0% |
| !! | TTTTGCTGTCTCTGCAGATG+GGG | - | chr8.4:9654443-9654462 | MS.gene26677:intron | 45.0% |
| AGATGGGGGGTCATTTGTGA+AGG | - | chr8.4:9654458-9654477 | MS.gene26677:intron | 50.0% | |
| ATATGCAGGTCGACACTGGT+CGG | + | chr8.4:9653535-9653554 | None:intergenic | 50.0% | |
| GAACCAAACCAGACATTCGG+AGG | + | chr8.4:9654408-9654427 | None:intergenic | 50.0% | |
| GATGGGGGGTCATTTGTGAA+GGG | - | chr8.4:9654459-9654478 | MS.gene26677:intron | 50.0% | |
| GGATCAGACACCGCAATACA+CGG | - | chr8.4:9654380-9654399 | MS.gene26677:CDS | 50.0% | |
| TGAAGATCACACTGCAGTGG+CGG | - | chr8.4:9654101-9654120 | MS.gene26677:intron | 50.0% | |
| ! | GCAGAGGCTACCACAGTTTT+TGG | - | chr8.4:9654136-9654155 | MS.gene26677:intron | 50.0% |
| ! | TTTGCTGTCTCTGCAGATGG+GGG | - | chr8.4:9654444-9654463 | MS.gene26677:intron | 50.0% |
| !! | TGCACCTGGTTTTCAGTTCC+AGG | - | chr8.4:9654530-9654549 | MS.gene26677:CDS | 50.0% |
| AGGCGAGACATGGTACCAGA+TGG | + | chr8.4:9653569-9653588 | None:intergenic | 55.0% | |
| CTGCAACCTCATCCAGAAGG+TGG | - | chr8.4:9653355-9653374 | MS.gene26677:CDS | 55.0% | |
| GTAGGATGCACTTGTGCACC+TGG | - | chr8.4:9654516-9654535 | MS.gene26677:CDS | 55.0% | |
| GTGCCTCCGAATGTCTGGTT+TGG | - | chr8.4:9654402-9654421 | MS.gene26677:CDS | 55.0% | |
| TTGCTGTCTCTGCAGATGGG+GGG | - | chr8.4:9654445-9654464 | MS.gene26677:intron | 55.0% | |
| !! | TTCGGAGGCACCGTGTATTG+CGG | + | chr8.4:9654393-9654412 | None:intergenic | 55.0% |
| ACACGGTGCCTCCGAATGTC+TGG | - | chr8.4:9654397-9654416 | MS.gene26677:CDS | 60.0% | |
| GCGGTGCAATGCTGTTGCAG+AGG | - | chr8.4:9654120-9654139 | MS.gene26677:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 9653316 | 9654638 | 9653316 | ID=MS.gene26677 |
| chr8.4 | mRNA | 9653316 | 9654638 | 9653316 | ID=MS.gene26677.t1;Parent=MS.gene26677 |
| chr8.4 | exon | 9654512 | 9654638 | 9654512 | ID=MS.gene26677.t1.exon1;Parent=MS.gene26677.t1 |
| chr8.4 | CDS | 9654512 | 9654638 | 9654512 | ID=cds.MS.gene26677.t1;Parent=MS.gene26677.t1 |
| chr8.4 | exon | 9654311 | 9654432 | 9654311 | ID=MS.gene26677.t1.exon2;Parent=MS.gene26677.t1 |
| chr8.4 | CDS | 9654311 | 9654432 | 9654311 | ID=cds.MS.gene26677.t1;Parent=MS.gene26677.t1 |
| chr8.4 | exon | 9653316 | 9653639 | 9653316 | ID=MS.gene26677.t1.exon3;Parent=MS.gene26677.t1 |
| chr8.4 | CDS | 9653316 | 9653639 | 9653316 | ID=cds.MS.gene26677.t1;Parent=MS.gene26677.t1 |
| Gene Sequence |
| Protein sequence |
