Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26687.t1 | XP_019418075.1 | 68.8 | 112 | 32 | 1 | 1 | 112 | 1 | 109 | 4.60E-35 | 157.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26687.t1 | Q9LUV2 | 61.6 | 112 | 40 | 1 | 1 | 112 | 1 | 109 | 9.1e-32 | 137.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26687.t1 | A0A151TDN5 | 67.0 | 112 | 34 | 1 | 1 | 112 | 1 | 109 | 1.7e-34 | 154.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056351 | MS.gene26687 | 0.816158 | 6.39E-52 | -1.69E-46 |
| MS.gene059512 | MS.gene26687 | 0.808895 | 2.47E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26687.t1 | MTR_4g124920 | 75.238 | 105 | 23 | 1 | 8 | 112 | 3 | 104 | 5.27e-50 | 154 |
| MS.gene26687.t1 | MTR_4g124920 | 73.611 | 72 | 16 | 1 | 8 | 79 | 3 | 71 | 8.13e-30 | 102 |
| MS.gene26687.t1 | MTR_2g084985 | 36.893 | 103 | 60 | 2 | 9 | 111 | 8 | 105 | 1.41e-14 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26687.t1 | AT3G17210 | 61.607 | 112 | 40 | 1 | 1 | 112 | 1 | 109 | 9.42e-45 | 141 |
| MS.gene26687.t1 | AT3G17210 | 61.607 | 112 | 40 | 1 | 1 | 112 | 1 | 109 | 9.42e-45 | 141 |
| MS.gene26687.t1 | AT5G22580 | 34.021 | 97 | 59 | 2 | 9 | 105 | 7 | 98 | 7.56e-15 | 65.5 |
Find 25 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGTTCCTCAATCTTCTCT+TGG | 0.329948 | 8.4:-9732368 | None:intergenic |
| GATTCAGGTAACAAATAATA+AGG | 0.348275 | 8.4:-9732686 | None:intergenic |
| AATGAGTAAATCCTTGATTC+AGG | 0.352373 | 8.4:-9732701 | None:intergenic |
| ATTATTTGTTACCTGAATCA+AGG | 0.432767 | 8.4:+9732690 | MS.gene26687:CDS |
| AAAGTTGCTCGTTCCTTGCT+TGG | 0.439365 | 8.4:+9732797 | MS.gene26687:CDS |
| GAGTTCCTCAATCTTCTCTT+GGG | 0.443255 | 8.4:-9732367 | None:intergenic |
| TTACCAATGGAATGACTTCA+AGG | 0.454151 | 8.4:-9732427 | None:intergenic |
| GTAGTGAAGAACATAGTGAT+TGG | 0.457715 | 8.4:+9732324 | MS.gene26687:CDS |
| TGGGATGAGATTGACGAGAT+TGG | 0.464836 | 8.4:-9732403 | None:intergenic |
| GAGGAAATGGAGGAAGCAAA+AGG | 0.502851 | 8.4:+9732300 | None:intergenic |
| CACCCTTGAAGTCATTCCAT+TGG | 0.506096 | 8.4:+9732424 | MS.gene26687:CDS |
| ATTCAAAGGTTAACGATGGT+GGG | 0.545431 | 8.4:-9732846 | None:intergenic |
| TCCTTGCTTGGAGAAAGTGC+TGG | 0.548785 | 8.4:+9732809 | MS.gene26687:CDS |
| TGAATCAACATTTGAGAGTG+TGG | 0.555452 | 8.4:+9732728 | MS.gene26687:CDS |
| TTTGCATACTCAACATGAGC+AGG | 0.558557 | 8.4:-9732777 | None:intergenic |
| GAAGATTGAGGAACTCATCA+AGG | 0.571882 | 8.4:+9732374 | MS.gene26687:CDS |
| GCCAGCACTTTCTCCAAGCA+AGG | 0.576667 | 8.4:-9732810 | None:intergenic |
| GATTCAAAGGTTAACGATGG+TGG | 0.578082 | 8.4:-9732847 | None:intergenic |
| GAGATTGACGAGATTGGCAA+AGG | 0.580515 | 8.4:-9732397 | None:intergenic |
| CAATGGAATGACTTCAAGGG+TGG | 0.596718 | 8.4:-9732423 | None:intergenic |
| AATCTCCCAAGAGAAGATTG+AGG | 0.597731 | 8.4:+9732362 | MS.gene26687:CDS |
| ATGAGCTACATACTCTGCAA+TGG | 0.614733 | 8.4:-9732754 | None:intergenic |
| TACCAATGGAATGACTTCAA+GGG | 0.647486 | 8.4:-9732426 | None:intergenic |
| AATGGAATGACTTCAAGGGT+GGG | 0.668406 | 8.4:-9732422 | None:intergenic |
| ACGGATTCAAAGGTTAACGA+TGG | 0.711712 | 8.4:-9732850 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTAAGTTTTTTAAGTTT+TGG | - | chr8.4:9732529-9732548 | None:intergenic | 10.0% |
| !!! | TATACATGTCTTATTTTTTT+AGG | + | chr8.4:9732646-9732665 | MS.gene26687:intron | 15.0% |
| ! | GATTCAGGTAACAAATAATA+AGG | - | chr8.4:9732689-9732708 | None:intergenic | 25.0% |
| !! | ATTATTTGTTACCTGAATCA+AGG | + | chr8.4:9732690-9732709 | MS.gene26687:CDS | 25.0% |
| !! | TTTAAGTTTCATCTTACCAA+TGG | - | chr8.4:9732443-9732462 | None:intergenic | 25.0% |
| AATGAGTAAATCCTTGATTC+AGG | - | chr8.4:9732704-9732723 | None:intergenic | 30.0% | |
| GTAGTGAAGAACATAGTGAT+TGG | + | chr8.4:9732324-9732343 | MS.gene26687:CDS | 35.0% | |
| TACCAATGGAATGACTTCAA+GGG | - | chr8.4:9732429-9732448 | None:intergenic | 35.0% | |
| TGAATCAACATTTGAGAGTG+TGG | + | chr8.4:9732728-9732747 | MS.gene26687:CDS | 35.0% | |
| TTACCAATGGAATGACTTCA+AGG | - | chr8.4:9732430-9732449 | None:intergenic | 35.0% | |
| !! | ATAGTCTAGACTGCTTTTGT+CGG | - | chr8.4:9732479-9732498 | None:intergenic | 35.0% |
| AATCTCCCAAGAGAAGATTG+AGG | + | chr8.4:9732362-9732381 | MS.gene26687:CDS | 40.0% | |
| AATGGAATGACTTCAAGGGT+GGG | - | chr8.4:9732425-9732444 | None:intergenic | 40.0% | |
| ATGAGCTACATACTCTGCAA+TGG | - | chr8.4:9732757-9732776 | None:intergenic | 40.0% | |
| GAAGATTGAGGAACTCATCA+AGG | + | chr8.4:9732374-9732393 | MS.gene26687:CDS | 40.0% | |
| GAGTTCCTCAATCTTCTCTT+GGG | - | chr8.4:9732370-9732389 | None:intergenic | 40.0% | |
| TGAGTTCCTCAATCTTCTCT+TGG | - | chr8.4:9732371-9732390 | None:intergenic | 40.0% | |
| TTTGCATACTCAACATGAGC+AGG | - | chr8.4:9732780-9732799 | None:intergenic | 40.0% | |
| AAAGTTGCTCGTTCCTTGCT+TGG | + | chr8.4:9732797-9732816 | MS.gene26687:CDS | 45.0% | |
| CAATGGAATGACTTCAAGGG+TGG | - | chr8.4:9732426-9732445 | None:intergenic | 45.0% | |
| CACCCTTGAAGTCATTCCAT+TGG | + | chr8.4:9732424-9732443 | MS.gene26687:CDS | 45.0% | |
| GAGATTGACGAGATTGGCAA+AGG | - | chr8.4:9732400-9732419 | None:intergenic | 45.0% | |
| TGGGATGAGATTGACGAGAT+TGG | - | chr8.4:9732406-9732425 | None:intergenic | 45.0% | |
| ! | GATTTTCGCTAAGTGGACGA+GGG | - | chr8.4:9732565-9732584 | None:intergenic | 45.0% |
| ! | TACCAGCGATTTTCGCTAAG+TGG | - | chr8.4:9732572-9732591 | None:intergenic | 45.0% |
| GTCCACTTAGCGAAAATCGC+TGG | + | chr8.4:9732567-9732586 | MS.gene26687:intron | 50.0% | |
| ! | CGATTTTCGCTAAGTGGACG+AGG | - | chr8.4:9732566-9732585 | None:intergenic | 50.0% |
| ! | TCCTTGCTTGGAGAAAGTGC+TGG | + | chr8.4:9732809-9732828 | MS.gene26687:CDS | 50.0% |
| GCCAGCACTTTCTCCAAGCA+AGG | - | chr8.4:9732813-9732832 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 9732306 | 9732866 | 9732306 | ID=MS.gene26687 |
| chr8.4 | mRNA | 9732306 | 9732866 | 9732306 | ID=MS.gene26687.t1;Parent=MS.gene26687 |
| chr8.4 | exon | 9732306 | 9732445 | 9732306 | ID=MS.gene26687.t1.exon1;Parent=MS.gene26687.t1 |
| chr8.4 | CDS | 9732306 | 9732445 | 9732306 | ID=cds.MS.gene26687.t1;Parent=MS.gene26687.t1 |
| chr8.4 | exon | 9732668 | 9732866 | 9732668 | ID=MS.gene26687.t1.exon2;Parent=MS.gene26687.t1 |
| chr8.4 | CDS | 9732668 | 9732866 | 9732668 | ID=cds.MS.gene26687.t1;Parent=MS.gene26687.t1 |
| Gene Sequence |
| Protein sequence |
