AlfalfaGEDB | MS.gene26762

M. sativa cultivar XinJiangDaYe / MS.gene26762

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26762.t1	XP_003609905.1	98.7	77	1	0	1	77	1	77	1.60E-34	154.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26762.t1	G7JR35	98.7	77	1	0	1	77	1	77	1.1e-34	154.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052077	MS.gene26762	0.814943	1.19E-51	-1.69E-46
MS.gene052176	MS.gene26762	0.808825	2.56E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26762.t1	MTR_4g124250	98.701	77	1	0	1	77	1	77	9.26e-53	158
MS.gene26762.t1	MTR_2g044940	77.922	77	13	3	1	76	1	74	4.00e-36	116

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26762.t1	AT1G48330	65.152	66	20	3	1	63	1	66	1.27e-23	85.1
MS.gene26762.t1	AT3G17580	60.274	73	17	4	1	63	1	71	3.28e-21	79.3

Find 18 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACACTCTGTGGCTGTGGATT+TGG	0.258834	8.3:+5113974	None:intergenic
AAACCTGTTTGAGACTTCTT+AGG	0.295118	8.3:+5114359	None:intergenic
ACCAAAACCAAATGGCTTCT+TGG	0.316100	8.3:-5114451	None:intergenic
TTCTTAGGATCCCTTGTTGT+TGG	0.351825	8.3:+5114374	None:intergenic
AGAACAAGAGAGGCGTATAA+TGG	0.401789	8.3:-5114066	MS.gene26762:CDS
TATGTTACACTCTGTGGCTG+TGG	0.492905	8.3:+5113968	None:intergenic
GATCCTAAGAAGTCTCAAAC+AGG	0.499805	8.3:-5114362	MS.gene26762:intron
TCAAGTTATGTGGTCAATGC+TGG	0.502431	8.3:-5114003	MS.gene26762:CDS
ATGAAGATGTTCAAGTTATG+TGG	0.553111	8.3:-5114013	MS.gene26762:CDS
GGATCTGCAAGGTGAAGTAA+TGG	0.564236	8.3:-5114045	MS.gene26762:CDS
TTATGTGGTCAATGCTGGAT+AGG	0.570689	8.3:-5113998	MS.gene26762:CDS
TATTGTTTACAGAACAAGAG+AGG	0.616356	8.3:-5114076	MS.gene26762:intron
TTAAAGAACAACCAACAACA+AGG	0.633465	8.3:-5114385	MS.gene26762:CDS
TGAAGTTATGTTACACTCTG+TGG	0.635521	8.3:+5113962	None:intergenic
CAAGGTGAAGTAATGGCATG+TGG	0.637142	8.3:-5114038	MS.gene26762:CDS
TAAAGAACAACCAACAACAA+GGG	0.675428	8.3:-5114384	MS.gene26762:CDS
AGGCGTATAATGGATCTGCA+AGG	0.696517	8.3:-5114056	MS.gene26762:CDS
AAGGTGAAGTAATGGCATGT+GGG	0.724189	8.3:-5114037	MS.gene26762:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAGTACAATTTTAAATTATA+AGG	+	chr8.3:5114212-5114231	None:intergenic	10.0%
!!!	GTACTTTTATTTATTTTTTA+CGG	-	chr8.3:5114226-5114245	MS.gene26762:intron	10.0%
!!	AAAAAATGAGTTAAATAATG+TGG	+	chr8.3:5114297-5114316	None:intergenic	15.0%
!!!	TTATTCAAATAGTATTTTGT+CGG	-	chr8.3:5114166-5114185	MS.gene26762:intron	15.0%
!!!	AAATGAAGATTTAAGATTGT+GGG	+	chr8.3:5114071-5114090	None:intergenic	20.0%
!!!	ATTGAAAGTACAATTTCTTT+TGG	+	chr8.3:5114273-5114292	None:intergenic	20.0%
!!	GAAATGAAGATTTAAGATTG+TGG	+	chr8.3:5114072-5114091	None:intergenic	25.0%
!!!	AAAGTACAAGCTTTTTTGAA+TGG	+	chr8.3:5113986-5114005	None:intergenic	25.0%
	TAAAGAACAACCAACAACAA+GGG	-	chr8.3:5114009-5114028	MS.gene26762:CDS	30.0%
	TATTGTTTACAGAACAAGAG+AGG	-	chr8.3:5114317-5114336	MS.gene26762:intron	30.0%
	TTAAAGAACAACCAACAACA+AGG	-	chr8.3:5114008-5114027	MS.gene26762:CDS	30.0%
!	ATGAAGATGTTCAAGTTATG+TGG	-	chr8.3:5114380-5114399	MS.gene26762:CDS	30.0%
	TGAAGTTATGTTACACTCTG+TGG	+	chr8.3:5114434-5114453	None:intergenic	35.0%
!	AAACCTGTTTGAGACTTCTT+AGG	+	chr8.3:5114037-5114056	None:intergenic	35.0%
!!!	TTTTTTGAATGGTGTTGCGA+TGG	+	chr8.3:5113975-5113994	None:intergenic	35.0%
	AAGGTGAAGTAATGGCATGT+GGG	-	chr8.3:5114356-5114375	MS.gene26762:intron	40.0%
	GATCCTAAGAAGTCTCAAAC+AGG	-	chr8.3:5114031-5114050	MS.gene26762:CDS	40.0%
	TCAAGTTATGTGGTCAATGC+TGG	-	chr8.3:5114390-5114409	MS.gene26762:CDS	40.0%
	TTATGTGGTCAATGCTGGAT+AGG	-	chr8.3:5114395-5114414	MS.gene26762:CDS	40.0%
!	AGAACAAGAGAGGCGTATAA+TGG	-	chr8.3:5114327-5114346	MS.gene26762:intron	40.0%
!	TTCTTAGGATCCCTTGTTGT+TGG	+	chr8.3:5114022-5114041	None:intergenic	40.0%
	CAAGGTGAAGTAATGGCATG+TGG	-	chr8.3:5114355-5114374	MS.gene26762:intron	45.0%
	GGATCTGCAAGGTGAAGTAA+TGG	-	chr8.3:5114348-5114367	MS.gene26762:intron	45.0%
	TATGTTACACTCTGTGGCTG+TGG	+	chr8.3:5114428-5114447	None:intergenic	45.0%
!!	AGGCGTATAATGGATCTGCA+AGG	-	chr8.3:5114337-5114356	MS.gene26762:intron	45.0%
	ACACTCTGTGGCTGTGGATT+TGG	+	chr8.3:5114422-5114441	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	5113953	5114462	5113953	ID=MS.gene26762
chr8.3	mRNA	5113953	5114462	5113953	ID=MS.gene26762.t1;Parent=MS.gene26762
chr8.3	exon	5114363	5114462	5114363	ID=MS.gene26762.t1.exon1;Parent=MS.gene26762.t1
chr8.3	CDS	5114363	5114462	5114363	ID=cds.MS.gene26762.t1;Parent=MS.gene26762.t1
chr8.3	exon	5113953	5114086	5113953	ID=MS.gene26762.t1.exon2;Parent=MS.gene26762.t1
chr8.3	CDS	5113953	5114086	5113953	ID=cds.MS.gene26762.t1;Parent=MS.gene26762.t1

Gene Sequence

>MS.gene26762

ATGGCTTCTTGGAAGAAAACCATCGCAACACCATTCAAAAAAGCTTGTACTTTCTTTAAAGAACAACCAACAACAAGGGATCCTAAGAAGTCTCAAACAGGTTTGTACATGTCATCCCACAATCTTAAATCTTCATTTCTTCGCTTAGACACTTCACAGATTTTTATTTTTTTTAATTATTTTCACAAATAAAAAAAAACACTTGTATGATAATTATTCAAATAGTATTTTGTCGGTTAATAGTACTATTATTATACCTTATAATTTAAAATTGTACTTTTATTTATTTTTTACGGAAAAATTGTACTCTTATAGAACCAAAAGAAATTGTACTTTCAATTCCACATTATTTAACTCATTTTTTTATTGTTTACAGAACAAGAGAGGCGTATAATGGATCTGCAAGGTGAAGTAATGGCATGTGGGTATGAAGATGTTCAAGTTATGTGGTCAATGCTGGATAGGTCCAAATCCACAGCCACAGAGTGTAACATAACTTCATCAAGTTGA

Protein sequence

>MS.gene26762.t1

MASWKKTIATPFKKACTFFKEQPTTRDPKKSQTEQERRIMDLQGEVMACGYEDVQVMWSMLDRSKSTATECNITSSS