Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26821.t1 | XP_013458324.1 | 99.1 | 113 | 1 | 0 | 1 | 113 | 366 | 478 | 3.30E-60 | 240.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26821.t1 | Q9LSM9 | 75.4 | 114 | 27 | 1 | 1 | 113 | 363 | 476 | 2.1e-47 | 189.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26821.t1 | A0A072V2P9 | 99.1 | 113 | 1 | 0 | 1 | 113 | 366 | 478 | 2.4e-60 | 240.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05872 | MS.gene26821 | 0.802315 | 5.95E-49 | -1.69E-46 |
| MS.gene061233 | MS.gene26821 | 0.952106 | 4.94E-110 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTATTCATTCTTTCCTAAC+TGG | 0.264101 | 8.1:+7969464 | MS.gene26821:CDS |
| CGATATTGTGTTGAGGCTTT+AGG | 0.279075 | 8.1:+7968755 | MS.gene26821:CDS |
| GCCTTAAGATATGGATTTAC+AGG | 0.283869 | 8.1:+7968293 | MS.gene26821:CDS |
| TTTGATGAGTTTGGTGTAGA+TGG | 0.323218 | 8.1:-7968266 | None:intergenic |
| AAGGCCACCTTTGATGAGTT+TGG | 0.339294 | 8.1:-7968275 | None:intergenic |
| GATCTCCAAGTAGACTTTAG+AGG | 0.351455 | 8.1:-7968782 | None:intergenic |
| AATAGAGGACCTGATTATTA+TGG | 0.363598 | 8.1:-7968811 | None:intergenic |
| TCAAAGGTGGCCTTAAGATA+TGG | 0.384822 | 8.1:+7968284 | MS.gene26821:CDS |
| GATGATCATGGACGTATAGT+AGG | 0.395363 | 8.1:-7969497 | None:intergenic |
| GAAGTTGTAAGTCCTCAATA+TGG | 0.403385 | 8.1:-7968224 | None:intergenic |
| GCAGGTTGGAGGAAGGATAG+TGG | 0.413209 | 8.1:+7969361 | MS.gene26821:intron |
| TAAAGTCTACTTGGAGATCT+TGG | 0.417999 | 8.1:+7968786 | MS.gene26821:CDS |
| ACCTGTAAATCCATATCTTA+AGG | 0.420489 | 8.1:-7968294 | None:intergenic |
| GATGGCAGACTACCTGTGAT+AGG | 0.427462 | 8.1:+7968728 | MS.gene26821:CDS |
| ATCTTGGTACCATAATAATC+AGG | 0.468032 | 8.1:+7968802 | MS.gene26821:CDS |
| GACTACCTCTAAAGTCTACT+TGG | 0.468519 | 8.1:+7968777 | MS.gene26821:CDS |
| TAGGGAGATGATCTCCAGTT+AGG | 0.484012 | 8.1:-7969478 | None:intergenic |
| GGAAGGATAGTGGAGTATGA+AGG | 0.491007 | 8.1:+7969371 | MS.gene26821:CDS |
| GCCTCAGTTGGTTTGTTGAG+AGG | 0.512490 | 8.1:-7969434 | None:intergenic |
| ATGAATGGAATGCAGGTTGG+AGG | 0.537003 | 8.1:+7969350 | MS.gene26821:intron |
| ATGGAATGCAGGTTGGAGGA+AGG | 0.549335 | 8.1:+7969354 | MS.gene26821:intron |
| TATGTGACAGTGAGAGGAGC+TGG | 0.554988 | 8.1:+7969401 | MS.gene26821:CDS |
| TGAGTTTGGTGTAGATGGGC+AGG | 0.558897 | 8.1:-7968261 | None:intergenic |
| ATGATCATGGACGTATAGTA+GGG | 0.561185 | 8.1:-7969496 | None:intergenic |
| TTGATGAGTTTGGTGTAGAT+GGG | 0.579382 | 8.1:-7968265 | None:intergenic |
| TTGACATATGTGACAGTGAG+AGG | 0.587941 | 8.1:+7969395 | MS.gene26821:CDS |
| CAATATCGTGTCCCTATCAC+AGG | 0.593552 | 8.1:-7968740 | None:intergenic |
| ATGGCAGACTACCTGTGATA+GGG | 0.603092 | 8.1:+7968729 | MS.gene26821:CDS |
| AGTGAGAGGAGCTGGTCACT+TGG | 0.603748 | 8.1:+7969409 | MS.gene26821:CDS |
| ATAAGAGAGAGAGCCTCAGT+TGG | 0.632043 | 8.1:-7969446 | None:intergenic |
| ATTAATAGTGGAGATGCAGA+TGG | 0.641384 | 8.1:+7968710 | MS.gene26821:intron |
| GCCTCTCAACAAACCAACTG+AGG | 0.642126 | 8.1:+7969433 | MS.gene26821:CDS |
| ATCTACACCAAACTCATCAA+AGG | 0.646779 | 8.1:+7968268 | MS.gene26821:CDS |
| TACACCAAACTCATCAAAGG+TGG | 0.666201 | 8.1:+7968271 | MS.gene26821:CDS |
| AGGGACACGATATTGTGTTG+AGG | 0.672514 | 8.1:+7968748 | MS.gene26821:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTAATAATTAATTTTTTTAA+AGG | + | chr8.1:7969075-7969094 | MS.gene26821:intron | 0.0% |
| !! | TTAATTAAGAATTTATACAA+TGG | - | chr8.1:7968848-7968867 | None:intergenic | 10.0% |
| !!! | TATCTTTTTTTTTTTTTTGA+GGG | + | chr8.1:7968438-7968457 | MS.gene26821:intron | 10.0% |
| !!! | TTAAATTCTAAAATTAATAG+AGG | + | chr8.1:7968560-7968579 | MS.gene26821:intron | 10.0% |
| !! | AATAATTAATTTCAACTAAG+AGG | + | chr8.1:7969102-7969121 | MS.gene26821:intron | 15.0% |
| !!! | CTATCTTTTTTTTTTTTTTG+AGG | + | chr8.1:7968437-7968456 | MS.gene26821:intron | 15.0% |
| !! | AAAATGCATTTATGACTTTA+TGG | + | chr8.1:7968527-7968546 | MS.gene26821:intron | 20.0% |
| !! | GTAAAAATACACAATCTATA+TGG | - | chr8.1:7968418-7968437 | None:intergenic | 20.0% |
| !! | TATATATCTATTCAGTCTTA+AGG | - | chr8.1:7968363-7968382 | None:intergenic | 20.0% |
| ! | AATACACAATCTATATGGTT+AGG | - | chr8.1:7968413-7968432 | None:intergenic | 25.0% |
| ! | ATAAGGATCCATATTGTTTA+TGG | + | chr8.1:7968488-7968507 | MS.gene26821:intron | 25.0% |
| ! | ATAGAATTGTTAGTAGCTTT+CGG | + | chr8.1:7968594-7968613 | MS.gene26821:intron | 25.0% |
| ! | ATGGCAAAAAAGATAAATAG+AGG | - | chr8.1:7968829-7968848 | None:intergenic | 25.0% |
| ! | CAACTAAGAGGTAAAATTAA+GGG | + | chr8.1:7969114-7969133 | MS.gene26821:intron | 25.0% |
| ! | CTATCTCATCTTTATAGATA+AGG | + | chr8.1:7968471-7968490 | MS.gene26821:intron | 25.0% |
| ! | TACTCTAAATTAATGTCGTT+AGG | + | chr8.1:7968333-7968352 | MS.gene26821:intron | 25.0% |
| ! | TCAACTAAGAGGTAAAATTA+AGG | + | chr8.1:7969113-7969132 | MS.gene26821:intron | 25.0% |
| !! | TAAGACTTAATGGATATGAA+TGG | + | chr8.1:7969335-7969354 | MS.gene26821:intron | 25.0% |
| !!! | AGAAAATTTTGAGTTTACAG+CGG | - | chr8.1:7969309-7969328 | None:intergenic | 25.0% |
| AATAGAGGACCTGATTATTA+TGG | - | chr8.1:7968814-7968833 | None:intergenic | 30.0% | |
| ACCTGTAAATCCATATCTTA+AGG | - | chr8.1:7968297-7968316 | None:intergenic | 30.0% | |
| ATCTTGGTACCATAATAATC+AGG | + | chr8.1:7968802-7968821 | MS.gene26821:CDS | 30.0% | |
| CACTATTAATTCATTCCACA+AGG | - | chr8.1:7968700-7968719 | None:intergenic | 30.0% | |
| CCATAATCAAGCTAGTAAAA+AGG | + | chr8.1:7969212-7969231 | MS.gene26821:intron | 30.0% | |
| CTTATTCATTCTTTCCTAAC+TGG | + | chr8.1:7969464-7969483 | MS.gene26821:CDS | 30.0% | |
| CTTGTGGAATGAATTAATAG+TGG | + | chr8.1:7968698-7968717 | MS.gene26821:intron | 30.0% | |
| TCATATCCATTAAGTCTTAG+AGG | - | chr8.1:7969334-7969353 | None:intergenic | 30.0% | |
| !! | CCTTTTTACTAGCTTGATTA+TGG | - | chr8.1:7969215-7969234 | None:intergenic | 30.0% |
| AATGGATATGAATGGAATGC+AGG | + | chr8.1:7969343-7969362 | MS.gene26821:intron | 35.0% | |
| ATCTACACCAAACTCATCAA+AGG | + | chr8.1:7968268-7968287 | MS.gene26821:CDS | 35.0% | |
| CACTTGGTCCATAAACAATA+TGG | - | chr8.1:7968499-7968518 | None:intergenic | 35.0% | |
| GAAGTTGTAAGTCCTCAATA+TGG | - | chr8.1:7968227-7968246 | None:intergenic | 35.0% | |
| GAGAATGAGGAAAATTACAG+TGG | + | chr8.1:7968956-7968975 | MS.gene26821:intron | 35.0% | |
| GAGTTACTTGATTGAGTTTG+TGG | + | chr8.1:7968988-7969007 | MS.gene26821:intron | 35.0% | |
| GCCTTAAGATATGGATTTAC+AGG | + | chr8.1:7968293-7968312 | MS.gene26821:CDS | 35.0% | |
| GTATGTTTGTTTGTACCTTG+TGG | + | chr8.1:7968682-7968701 | MS.gene26821:intron | 35.0% | |
| TAAAGTCTACTTGGAGATCT+TGG | + | chr8.1:7968786-7968805 | MS.gene26821:CDS | 35.0% | |
| ! | ATTAATAGTGGAGATGCAGA+TGG | + | chr8.1:7968710-7968729 | MS.gene26821:intron | 35.0% |
| ! | TGCATTTTTCACTTTGCACT+TGG | - | chr8.1:7968515-7968534 | None:intergenic | 35.0% |
| !! | TTCTCTCCTCTAAGACTTAA+TGG | + | chr8.1:7969325-7969344 | MS.gene26821:intron | 35.0% |
| !! | TTGATGAGTTTGGTGTAGAT+GGG | - | chr8.1:7968268-7968287 | None:intergenic | 35.0% |
| !! | TTTGATGAGTTTGGTGTAGA+TGG | - | chr8.1:7968269-7968288 | None:intergenic | 35.0% |
| !!! | CGCATTTTGATTAGAGAATG+AGG | + | chr8.1:7968943-7968962 | MS.gene26821:intron | 35.0% |
| CGATATTGTGTTGAGGCTTT+AGG | + | chr8.1:7968755-7968774 | MS.gene26821:CDS | 40.0% | |
| GATATGAATGGAATGCAGGT+TGG | + | chr8.1:7969347-7969366 | MS.gene26821:intron | 40.0% | |
| GATCTCCAAGTAGACTTTAG+AGG | - | chr8.1:7968785-7968804 | None:intergenic | 40.0% | |
| GTTAGTAGCTTTCGGTGAAA+GGG | + | chr8.1:7968602-7968621 | MS.gene26821:intron | 40.0% | |
| TACACCAAACTCATCAAAGG+TGG | + | chr8.1:7968271-7968290 | MS.gene26821:CDS | 40.0% | |
| TGTTAGTAGCTTTCGGTGAA+AGG | + | chr8.1:7968601-7968620 | MS.gene26821:intron | 40.0% | |
| TTGACATATGTGACAGTGAG+AGG | + | chr8.1:7969395-7969414 | MS.gene26821:CDS | 40.0% | |
| ! | TCAAAGGTGGCCTTAAGATA+TGG | + | chr8.1:7968284-7968303 | MS.gene26821:CDS | 40.0% |
| !! | GACTACCTCTAAAGTCTACT+TGG | + | chr8.1:7968777-7968796 | MS.gene26821:CDS | 40.0% |
| AAGGCCACCTTTGATGAGTT+TGG | - | chr8.1:7968278-7968297 | None:intergenic | 45.0% | |
| AGGGACACGATATTGTGTTG+AGG | + | chr8.1:7968748-7968767 | MS.gene26821:CDS | 45.0% | |
| ATAAGAGAGAGAGCCTCAGT+TGG | - | chr8.1:7969449-7969468 | None:intergenic | 45.0% | |
| ATGGCAGACTACCTGTGATA+GGG | + | chr8.1:7968729-7968748 | MS.gene26821:CDS | 45.0% | |
| CAATATCGTGTCCCTATCAC+AGG | - | chr8.1:7968743-7968762 | None:intergenic | 45.0% | |
| TAGGGAGATGATCTCCAGTT+AGG | - | chr8.1:7969481-7969500 | None:intergenic | 45.0% | |
| ! | ATGAATGGAATGCAGGTTGG+AGG | + | chr8.1:7969350-7969369 | MS.gene26821:intron | 45.0% |
| !! | GGAAGGATAGTGGAGTATGA+AGG | + | chr8.1:7969371-7969390 | MS.gene26821:CDS | 45.0% |
| GATGGCAGACTACCTGTGAT+AGG | + | chr8.1:7968728-7968747 | MS.gene26821:CDS | 50.0% | |
| GCCTCTCAACAAACCAACTG+AGG | + | chr8.1:7969433-7969452 | MS.gene26821:CDS | 50.0% | |
| TATGTGACAGTGAGAGGAGC+TGG | + | chr8.1:7969401-7969420 | MS.gene26821:CDS | 50.0% | |
| ! | ATGGAATGCAGGTTGGAGGA+AGG | + | chr8.1:7969354-7969373 | MS.gene26821:intron | 50.0% |
| !! | GCCTCAGTTGGTTTGTTGAG+AGG | - | chr8.1:7969437-7969456 | None:intergenic | 50.0% |
| !! | TGAGTTTGGTGTAGATGGGC+AGG | - | chr8.1:7968264-7968283 | None:intergenic | 50.0% |
| AGTGAGAGGAGCTGGTCACT+TGG | + | chr8.1:7969409-7969428 | MS.gene26821:CDS | 55.0% | |
| ! | GCAGGTTGGAGGAAGGATAG+TGG | + | chr8.1:7969361-7969380 | MS.gene26821:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 7968219 | 7969514 | 7968219 | ID=MS.gene26821 |
| chr8.1 | mRNA | 7968219 | 7969514 | 7968219 | ID=MS.gene26821.t1;Parent=MS.gene26821 |
| chr8.1 | exon | 7968219 | 7968314 | 7968219 | ID=MS.gene26821.t1.exon1;Parent=MS.gene26821.t1 |
| chr8.1 | CDS | 7968219 | 7968314 | 7968219 | ID=cds.MS.gene26821.t1;Parent=MS.gene26821.t1 |
| chr8.1 | exon | 7968718 | 7968823 | 7968718 | ID=MS.gene26821.t1.exon2;Parent=MS.gene26821.t1 |
| chr8.1 | CDS | 7968718 | 7968823 | 7968718 | ID=cds.MS.gene26821.t1;Parent=MS.gene26821.t1 |
| chr8.1 | exon | 7969365 | 7969514 | 7969365 | ID=MS.gene26821.t1.exon3;Parent=MS.gene26821.t1 |
| chr8.1 | CDS | 7969365 | 7969514 | 7969365 | ID=cds.MS.gene26821.t1;Parent=MS.gene26821.t1 |
| Gene Sequence |
| Protein sequence |
