AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27293

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27293.t1 MTR_1g022400 98.020 404 8 0 1 404 1 404 0.0 823
MS.gene27293.t1 MTR_1g072300 33.989 356 228 5 48 399 30 382 2.77e-66 219
MS.gene27293.t1 MTR_3g099753 29.843 382 263 4 23 401 29 408 1.99e-54 188
MS.gene27293.t1 MTR_3g099753 30.372 349 239 3 55 401 38 384 2.29e-54 188
MS.gene27293.t1 MTR_8g069570 33.438 317 208 3 74 390 43 356 1.92e-51 179
MS.gene27293.t1 MTR_8g465890 31.016 374 237 8 18 387 4 360 1.27e-44 161
MS.gene27293.t1 MTR_2g090290 29.204 339 230 5 52 383 30 365 5.34e-44 159
MS.gene27293.t1 MTR_2g047855 30.959 365 234 7 24 387 14 361 1.98e-43 158
MS.gene27293.t1 MTR_1g086820 29.814 322 219 5 38 359 18 332 2.16e-43 158
MS.gene27293.t1 MTR_2g013450 30.405 296 204 2 74 368 48 342 1.03e-42 156
MS.gene27293.t1 MTR_8g059115 30.986 284 189 5 120 401 9 287 1.58e-34 132
MS.gene27293.t1 MTR_8g007375 27.600 250 180 1 116 365 6 254 1.48e-32 127
MS.gene27293.t1 MTR_8g031520 32.075 212 140 3 39 249 92 300 2.27e-31 121
MS.gene27293.t1 MTR_7g089770 28.041 296 199 7 73 362 175 462 2.13e-28 117
MS.gene27293.t1 MTR_2g096880 23.410 346 236 8 13 354 20 340 6.72e-23 100
MS.gene27293.t1 MTR_1g050750 27.365 296 203 7 69 359 105 393 1.14e-22 100
MS.gene27293.t1 MTR_2g036560 23.958 288 195 6 87 360 164 441 6.88e-15 77.0
MS.gene27293.t1 MTR_3g033040 23.077 338 251 7 62 395 144 476 1.05e-14 76.3
MS.gene27293.t1 MTR_3g033040 23.077 338 251 7 62 395 144 476 1.39e-14 75.5
MS.gene27293.t1 MTR_7g017530 24.898 245 181 3 157 401 286 527 6.24e-14 74.3
MS.gene27293.t1 MTR_5g008300 23.558 208 157 2 157 364 373 578 3.95e-12 68.9
MS.gene27293.t1 MTR_7g017570 31.061 132 85 2 125 252 357 486 7.28e-12 67.8
MS.gene27293.t1 MTR_7g017570 23.673 245 184 3 157 401 321 562 1.63e-11 66.6
MS.gene27293.t1 MTR_2g030540 28.395 162 111 3 156 317 396 552 6.71e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27293.t1 AT5G09450 56.479 409 168 5 1 403 1 405 5.48e-169 479
MS.gene27293.t1 AT4G02820 33.058 363 231 7 45 401 48 404 7.34e-61 206
MS.gene27293.t1 AT5G27460 30.423 378 254 4 31 401 12 387 6.22e-53 184
MS.gene27293.t1 AT2G20710 30.312 353 242 4 46 397 30 379 9.22e-52 181
MS.gene27293.t1 AT1G60770 34.098 305 194 4 88 390 57 356 2.05e-49 174
MS.gene27293.t1 AT4G21705 29.834 362 230 4 8 368 1 339 3.10e-47 168
MS.gene27293.t1 AT1G02150 29.118 340 238 3 66 404 79 416 4.22e-46 166
MS.gene27293.t1 AT1G02370 28.254 315 223 3 46 359 63 375 1.17e-41 154
MS.gene27293.t1 AT4G01990 30.405 296 203 3 73 368 59 351 1.53e-41 153
MS.gene27293.t1 AT1G28020 30.341 323 215 6 73 390 55 372 1.71e-35 136
MS.gene27293.t1 AT1G28020 36.036 111 68 1 56 166 373 480 2.55e-14 75.1
MS.gene27293.t1 AT2G20710 27.526 287 204 4 112 397 1 284 8.69e-34 130
MS.gene27293.t1 AT1G80270 26.299 308 217 5 66 369 140 441 3.03e-28 117
MS.gene27293.t1 AT1G80270 26.299 308 217 5 66 369 140 441 3.03e-28 117
MS.gene27293.t1 AT1G80270 26.299 308 217 5 66 369 140 441 3.03e-28 117
MS.gene27293.t1 AT1G80270 26.299 308 217 5 66 369 140 441 3.03e-28 117
MS.gene27293.t1 AT1G80270 26.299 308 217 5 66 369 140 441 3.03e-28 117
MS.gene27293.t1 AT1G15480 26.552 290 205 6 69 357 141 423 7.20e-24 104
MS.gene27293.t1 AT3G15590 25.547 274 195 6 47 319 138 403 3.34e-23 102
MS.gene27293.t1 AT3G15590 25.547 274 195 6 47 319 138 403 3.34e-23 102
MS.gene27293.t1 AT3G11380 31.633 196 132 2 54 247 346 541 1.26e-22 100
MS.gene27293.t1 AT3G11380 26.623 308 187 7 87 390 76 348 1.34e-20 94.4
MS.gene27293.t1 AT1G07590 25.532 329 237 6 1 324 8 333 1.78e-22 99.8
MS.gene27293.t1 AT1G28000 27.111 225 153 4 58 275 37 257 7.02e-19 88.2
MS.gene27293.t1 AT1G43010 23.774 265 174 7 27 280 9 256 2.12e-15 75.9
MS.gene27293.t1 AT5G02860 23.755 261 192 5 87 342 136 394 1.55e-13 72.8
MS.gene27293.t1 AT5G02860 23.755 261 192 5 87 342 136 394 1.55e-13 72.8
MS.gene27293.t1 AT5G02860 23.755 261 192 5 87 342 136 394 1.55e-13 72.8
MS.gene27293.t1 AT5G02860 23.755 261 192 5 87 342 136 394 1.55e-13 72.8
MS.gene27293.t1 AT5G02860 23.755 261 192 5 87 342 136 394 1.55e-13 72.8
MS.gene27293.t1 AT3G11350 30.000 140 75 3 99 215 53 192 2.77e-12 68.2
MS.gene27293.t1 AT2G18940 24.815 270 179 9 87 342 137 396 2.06e-11 66.2
MS.gene27293.t1 AT1G74850 20.538 409 300 10 14 402 18 421 6.68e-11 64.7

Find 75 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATGCTGGGTTGGGAAATA+AGG 0.236043 1.2:+2151102 MS.gene27293:CDS
AACATTAGTAGCACTTCTTT+TGG 0.246404 1.2:-2148978 None:intergenic
GCTTGATTGTAAAAGGATTA+TGG 0.252184 1.2:+2151288 MS.gene27293:CDS
AGGATAAACTTGATCAAATA+TGG 0.253390 1.2:+2151122 MS.gene27293:CDS
TTGATGTGCTTGATTGTAAA+AGG 0.262901 1.2:+2151281 MS.gene27293:CDS
ATGGATAACATATGACTTAC+TGG 0.307051 1.2:+2151072 MS.gene27293:CDS
AGATTAAAAGTAAAGATATC+AGG 0.320239 1.2:-2150851 None:intergenic
TTACTGGTCATTCTTTATGC+TGG 0.340583 1.2:+2151088 MS.gene27293:CDS
TTAATATTGATGAAGTTAGA+AGG 0.362686 1.2:+2150900 MS.gene27293:CDS
GTCATTCTTTATGCTGGGTT+GGG 0.371727 1.2:+2151094 MS.gene27293:CDS
TACTGGTCATTCTTTATGCT+GGG 0.385252 1.2:+2151089 MS.gene27293:CDS
GGTCATTCTTTATGCTGGGT+TGG 0.385582 1.2:+2151093 MS.gene27293:CDS
TCATGAAGAATATGAGTTAT+CGG 0.398822 1.2:+2150526 MS.gene27293:CDS
GAGAATCATATTCAAGTTGT+TGG 0.401757 1.2:-2151344 None:intergenic
CAGAGGGTGATGATGATCTA+AGG 0.409395 1.2:+2148930 MS.gene27293:CDS
GTAATTATTGCTGATTAAAC+TGG 0.412423 1.2:-2148828 None:intergenic
TCAGCGTTATAAACACGCTT+TGG 0.414368 1.2:+2149078 MS.gene27293:CDS
GTGATATTTCTAAAGAGCTT+AGG 0.447878 1.2:+2149050 MS.gene27293:CDS
TATCCTTTCATATAGTTCCT+CGG 0.457583 1.2:-2150705 None:intergenic
TCATCACCCTCTGCAGAGTT+CGG 0.464784 1.2:-2148920 None:intergenic
TGAAGACCTCAAACAACAAA+AGG 0.466712 1.2:+2150823 MS.gene27293:CDS
ATATCACGGAGCTCAGAAAC+AGG 0.468593 1.2:-2149034 None:intergenic
TCTTTACATGTCAGTTGGGC+AGG 0.472806 1.2:+2150778 MS.gene27293:CDS
TTGTACGTAACGGCATCAAA+GGG 0.473448 1.2:-2150743 None:intergenic
GCGTTATAAACACGCTTTGG+AGG 0.476273 1.2:+2149081 MS.gene27293:CDS
CTAAACAGAAAATGATTAGC+AGG 0.484955 1.2:+2151161 MS.gene27293:CDS
CAGGTGAAGTTATTGATCAA+TGG 0.491061 1.2:+2151236 MS.gene27293:CDS
ATGACTCTTTACATGTCAGT+TGG 0.492710 1.2:+2150773 MS.gene27293:CDS
ATTGTACGTAACGGCATCAA+AGG 0.498103 1.2:-2150744 None:intergenic
GCCCTCCTCCATTCTTATGC+AGG 0.500197 1.2:+2150662 MS.gene27293:CDS
CTCAGTCTCGACGAGTGAGT+TGG 0.502370 1.2:-2151029 None:intergenic
AAGGGATCACACAAAGTCAA+TGG 0.508969 1.2:+2151053 MS.gene27293:CDS
CTTGATCATGCAAAAGAAGC+AGG 0.509186 1.2:+2151217 MS.gene27293:CDS
TGACTCTTTACATGTCAGTT+GGG 0.520810 1.2:+2150774 MS.gene27293:CDS
CCGCATAGATTTGATGAACA+AGG 0.522273 1.2:+2150565 MS.gene27293:CDS
GTGCTGGTTCTGATGAAAGT+TGG 0.524579 1.2:+2150945 MS.gene27293:CDS
TCACTCGTCGAGACTGAGAA+AGG 0.530019 1.2:+2151034 MS.gene27293:CDS
CATTGAGAAGAACCTCAATC+CGG 0.538432 1.2:+2151366 MS.gene27293:CDS
CAGTGAAATATACGTTGGCC+AGG 0.538859 1.2:-2150982 None:intergenic
CTCCTGCATAAGAATGGAGG+AGG 0.542649 1.2:-2150664 None:intergenic
TTGAGGTCTTCAACAACCGA+TGG 0.554094 1.2:-2150812 None:intergenic
TGCTGTGACCAGTGATTATG+TGG 0.559146 1.2:+2148889 MS.gene27293:CDS
GAAGAAATGCGTAATGGTGC+TGG 0.559211 1.2:+2150929 MS.gene27293:CDS
ATTTCAGATATCAGAATGGA+TGG 0.560128 1.2:+2150499 MS.gene27293:intron
GATTAAAAGTAAAGATATCA+GGG 0.567372 1.2:-2150850 None:intergenic
CACTCGTCGAGACTGAGAAA+GGG 0.570011 1.2:+2151035 MS.gene27293:CDS
AGTCTCTTCCACATAATCAC+TGG 0.571573 1.2:-2148897 None:intergenic
CATCATTTCATTGTACGTAA+CGG 0.576928 1.2:-2150753 None:intergenic
GCCAACGTATATTTCACTGC+GGG 0.584073 1.2:+2150986 MS.gene27293:CDS
ATTCTTGAAGAAATGCGTAA+TGG 0.587763 1.2:+2150923 MS.gene27293:CDS
TTGCTCCTGCATAAGAATGG+AGG 0.600601 1.2:-2150667 None:intergenic
TAAGCTCTTTAGAAATATCA+CGG 0.600859 1.2:-2149048 None:intergenic
AGGCCGAGGAACTATATGAA+AGG 0.606287 1.2:+2150702 MS.gene27293:CDS
TGGAACTTGCACTATCAAGA+TGG 0.610177 1.2:-2151009 None:intergenic
TGAGGTCTTCAACAACCGAT+GGG 0.611229 1.2:-2150811 None:intergenic
TCGCAAGTCTTTGCAGAGGG+TGG 0.611538 1.2:-2150629 None:intergenic
GTCTCGCAAGTCTTTGCAGA+GGG 0.613659 1.2:-2150632 None:intergenic
AGAGACTCCGAACTCTGCAG+AGG 0.614041 1.2:+2148913 MS.gene27293:CDS
TTGGATTAGATATTTGAACC+TGG 0.618204 1.2:+2150964 MS.gene27293:CDS
GATGATTTCAGATATCAGAA+TGG 0.621758 1.2:+2150495 MS.gene27293:intron
GAGACTCCGAACTCTGCAGA+GGG 0.624222 1.2:+2148914 MS.gene27293:CDS
TTCTCTCTCTCATCAAACGA+AGG 0.627248 1.2:+2148706 MS.gene27293:CDS
AAAGACTTGCGAGACTTACA+CGG 0.633932 1.2:+2150640 MS.gene27293:CDS
ATCAAGCACATCAAAATCTG+TGG 0.635067 1.2:-2151272 None:intergenic
ATCTAAGGAGTAGAATCTTG+AGG 0.636582 1.2:+2148945 MS.gene27293:CDS
GCCCGCAGTGAAATATACGT+TGG 0.643301 1.2:-2150987 None:intergenic
ACGTAACGGCATCAAAGGGG+AGG 0.670188 1.2:-2150739 None:intergenic
GCAGGTAGAAAAGATCCCAT+CGG 0.682540 1.2:+2150796 MS.gene27293:CDS
TCCTGCATAAGAATGGAGGA+GGG 0.685369 1.2:-2150663 None:intergenic
AGTCTCGCAAGTCTTTGCAG+AGG 0.690456 1.2:-2150633 None:intergenic
GGCCAACGTATATTTCACTG+CGG 0.706091 1.2:+2150985 MS.gene27293:CDS
TGAGAGAGAGAAATAGAGAG+CGG 0.708192 1.2:-2148695 None:intergenic
CCTTGTTCATCAAATCTATG+CGG 0.713202 1.2:-2150565 None:intergenic
TGTACGTAACGGCATCAAAG+GGG 0.718003 1.2:-2150742 None:intergenic
TTGCTGAACGATGATTGTGA+TGG 0.744152 1.2:-2148850 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTGAATTAATATTGTT+CGG + chr1.2:2150389-2150408 MS.gene27293:intron 10.0%
!! ATCAAAATTATCAAATATGT+CGG - chr1.2:2149461-2149480 None:intergenic 15.0%
!!! ATATCTTTACTTTTAATCTA+TGG + chr1.2:2150855-2150874 MS.gene27293:CDS 15.0%
!!! TCATTTTCTCAAATAATATT+TGG - chr1.2:2150139-2150158 None:intergenic 15.0%
!! AGATTAAAAGTAAAGATATC+AGG - chr1.2:2150854-2150873 None:intergenic 20.0%
!! ATGATTATTAATAGCTAATG+AGG + chr1.2:2150073-2150092 MS.gene27293:intron 20.0%
!! GATTAAAAGTAAAGATATCA+GGG - chr1.2:2150853-2150872 None:intergenic 20.0%
!! TACTAATGTTCTTCAAAAAT+GGG + chr1.2:2148991-2149010 MS.gene27293:CDS 20.0%
!! TTAATATTGATGAAGTTAGA+AGG + chr1.2:2150900-2150919 MS.gene27293:CDS 20.0%
!! TTCAAAATAATATTCACTTC+TGG - chr1.2:2150378-2150397 None:intergenic 20.0%
!!! ATTAGTTTTGCTCTATAAAA+CGG + chr1.2:2150452-2150471 MS.gene27293:intron 20.0%
! AATTAAGCACTTATGGAATA+TGG - chr1.2:2149794-2149813 None:intergenic 25.0%
! AGGATAAACTTGATCAAATA+TGG + chr1.2:2151122-2151141 MS.gene27293:CDS 25.0%
! ATATGAAACTAAGCTGAAAA+TGG + chr1.2:2149671-2149690 MS.gene27293:intron 25.0%
! CCAATAATTTGAATCTGATT+GGG - chr1.2:2148776-2148795 None:intergenic 25.0%
! CCAATCAGATTCAAATTATT+GGG + chr1.2:2148774-2148793 MS.gene27293:intron 25.0%
! CTACTAATGTTCTTCAAAAA+TGG + chr1.2:2148990-2149009 MS.gene27293:CDS 25.0%
! TCATGAAGAATATGAGTTAT+CGG + chr1.2:2150526-2150545 MS.gene27293:CDS 25.0%
! TGAAATAAGCTAATGAGTAA+TGG - chr1.2:2149215-2149234 None:intergenic 25.0%
! TTGTCTTGATTACTAATACA+AGG - chr1.2:2150036-2150055 None:intergenic 25.0%
!! AAGGATTATGGATTCTTTTA+CGG + chr1.2:2151300-2151319 MS.gene27293:CDS 25.0%
!! GTAATTATTGCTGATTAAAC+TGG - chr1.2:2148831-2148850 None:intergenic 25.0%
!! TAAGCTCTTTAGAAATATCA+CGG - chr1.2:2149051-2149070 None:intergenic 25.0%
AGTTCTCTGATTGTGAATTA+GGG + chr1.2:2149894-2149913 MS.gene27293:intron 30.0%
ATGGATAACATATGACTTAC+TGG + chr1.2:2151072-2151091 MS.gene27293:CDS 30.0%
ATTCTTGAAGAAATGCGTAA+TGG + chr1.2:2150923-2150942 MS.gene27293:CDS 30.0%
ATTTCAGATATCAGAATGGA+TGG + chr1.2:2150499-2150518 MS.gene27293:intron 30.0%
CATCATTTCATTGTACGTAA+CGG - chr1.2:2150756-2150775 None:intergenic 30.0%
CCCAATAATTTGAATCTGAT+TGG - chr1.2:2148777-2148796 None:intergenic 30.0%
CCCAATCAGATTCAAATTAT+TGG + chr1.2:2148773-2148792 MS.gene27293:intron 30.0%
CTAAACAGAAAATGATTAGC+AGG + chr1.2:2151161-2151180 MS.gene27293:CDS 30.0%
CTTGTAACTGTTGCAATAAA+AGG + chr1.2:2150270-2150289 MS.gene27293:intron 30.0%
GATGATTTCAGATATCAGAA+TGG + chr1.2:2150495-2150514 MS.gene27293:intron 30.0%
GCTTGATTGTAAAAGGATTA+TGG + chr1.2:2151288-2151307 MS.gene27293:CDS 30.0%
TAGTTCTCTGATTGTGAATT+AGG + chr1.2:2149893-2149912 MS.gene27293:intron 30.0%
TATCCTTTCATATAGTTCCT+CGG - chr1.2:2150708-2150727 None:intergenic 30.0%
TATTAATAGCTAATGAGGCA+CGG + chr1.2:2150078-2150097 MS.gene27293:intron 30.0%
TGAGAAAATTGGAGAAAGTT+GGG - chr1.2:2148750-2148769 None:intergenic 30.0%
TGTGAATTAGGGATAATTTC+TGG + chr1.2:2149905-2149924 MS.gene27293:intron 30.0%
TTGAGAAAATTGGAGAAAGT+TGG - chr1.2:2148751-2148770 None:intergenic 30.0%
TTGATGTGCTTGATTGTAAA+AGG + chr1.2:2151281-2151300 MS.gene27293:CDS 30.0%
! AACATTAGTAGCACTTCTTT+TGG - chr1.2:2148981-2149000 None:intergenic 30.0%
! ACATTAGTAGCACTTCTTTT+GGG - chr1.2:2148980-2148999 None:intergenic 30.0%
! ATGGATTCTTTTACGGAAAT+TGG + chr1.2:2151307-2151326 MS.gene27293:CDS 30.0%
! CAGAGATTAATAGTTCTGTA+AGG + chr1.2:2150213-2150232 MS.gene27293:intron 30.0%
! GATTTGATGAACAAGGTTTT+TGG + chr1.2:2150572-2150591 MS.gene27293:CDS 30.0%
! GTGATATTTCTAAAGAGCTT+AGG + chr1.2:2149050-2149069 MS.gene27293:CDS 30.0%
! TTAGGGATAATTTCTGGAAA+TGG + chr1.2:2149911-2149930 MS.gene27293:intron 30.0%
! TTCAAAAATGGGTTCTTCAA+GGG + chr1.2:2149002-2149021 MS.gene27293:CDS 30.0%
! TTCTTTTACGGAAATTGGAT+TGG + chr1.2:2151312-2151331 MS.gene27293:CDS 30.0%
! TTGGATTAGATATTTGAACC+TGG + chr1.2:2150964-2150983 MS.gene27293:CDS 30.0%
!! AACGAGTTCTGAAGTTTATT+TGG + chr1.2:2149863-2149882 MS.gene27293:intron 30.0%
!! AAGTTGTTTTCATATCCTAG+AGG + chr1.2:2149646-2149665 MS.gene27293:intron 30.0%
!! ACGAGTTCTGAAGTTTATTT+GGG + chr1.2:2149864-2149883 MS.gene27293:intron 30.0%
!! AGTTGTTTTCATATCCTAGA+GGG + chr1.2:2149647-2149666 MS.gene27293:intron 30.0%
!! GAGAATCATATTCAAGTTGT+TGG - chr1.2:2151347-2151366 None:intergenic 30.0%
AACTAAGCTGAAAATGGCTT+AGG + chr1.2:2149677-2149696 MS.gene27293:intron 35.0%
ACAGCTCAATTAAGCACTTA+TGG - chr1.2:2149801-2149820 None:intergenic 35.0%
ATCAAGCACATCAAAATCTG+TGG - chr1.2:2151275-2151294 None:intergenic 35.0%
ATCCAAACAGAGAAAAGACA+GGG - chr1.2:2149112-2149131 None:intergenic 35.0%
ATCTAAGGAGTAGAATCTTG+AGG + chr1.2:2148945-2148964 MS.gene27293:CDS 35.0%
ATGACTCTTTACATGTCAGT+TGG + chr1.2:2150773-2150792 MS.gene27293:CDS 35.0%
CAGGTGAAGTTATTGATCAA+TGG + chr1.2:2151236-2151255 MS.gene27293:CDS 35.0%
CCTTGTTCATCAAATCTATG+CGG - chr1.2:2150568-2150587 None:intergenic 35.0%
GAATATGGACATGTCTGTAT+TGG - chr1.2:2149779-2149798 None:intergenic 35.0%
GGCTTATTTGAGCTTAACTA+CGG - chr1.2:2149758-2149777 None:intergenic 35.0%
GTTAAATCTGAGTGCATGTT+AGG + chr1.2:2150329-2150348 MS.gene27293:intron 35.0%
TACTGGTCATTCTTTATGCT+GGG + chr1.2:2151089-2151108 MS.gene27293:CDS 35.0%
TGAAGACCTCAAACAACAAA+AGG + chr1.2:2150823-2150842 MS.gene27293:CDS 35.0%
TGACTCTTTACATGTCAGTT+GGG + chr1.2:2150774-2150793 MS.gene27293:CDS 35.0%
TGTTGTTCAGTGCTAACTAA+GGG + chr1.2:2150301-2150320 MS.gene27293:intron 35.0%
TTACTGGTCATTCTTTATGC+TGG + chr1.2:2151088-2151107 MS.gene27293:CDS 35.0%
! AAACTGGTGTTTCTGCAAAA+TGG - chr1.2:2148815-2148834 None:intergenic 35.0%
! CATTAGTAGCACTTCTTTTG+GGG - chr1.2:2148979-2148998 None:intergenic 35.0%
! CTTATTTCAGTTAGCTCTCT+AGG + chr1.2:2149226-2149245 MS.gene27293:intron 35.0%
! CTTCAAAAATGGGTTCTTCA+AGG + chr1.2:2149001-2149020 MS.gene27293:CDS 35.0%
! TTCACTTCTGGAAACTTCTT+TGG - chr1.2:2150366-2150385 None:intergenic 35.0%
! TTTTTGCTCCTGCATAAGAA+TGG - chr1.2:2150673-2150692 None:intergenic 35.0%
!! CGAGTTCTGAAGTTTATTTG+GGG + chr1.2:2149865-2149884 MS.gene27293:intron 35.0%
!! TGAGTAATGGACATGTCATA+AGG - chr1.2:2149202-2149221 None:intergenic 35.0%
!! TGATTGGGTGTTGAGAAAAT+TGG - chr1.2:2148761-2148780 None:intergenic 35.0%
AAACTTCTTTGGCGTTGCAA+TGG - chr1.2:2150355-2150374 None:intergenic 40.0%
AAAGACTTGCGAGACTTACA+CGG + chr1.2:2150640-2150659 MS.gene27293:CDS 40.0%
AAGGGATCACACAAAGTCAA+TGG + chr1.2:2151053-2151072 MS.gene27293:CDS 40.0%
AATCGTTGTCTTGTCAGTGT+TGG + chr1.2:2150181-2150200 MS.gene27293:intron 40.0%
ACACTGACACATCTAATCGA+AGG + chr1.2:2149406-2149425 MS.gene27293:intron 40.0%
AGGAGCAAAAATGACCGAAA+AGG + chr1.2:2150682-2150701 MS.gene27293:CDS 40.0%
AGTCTCTTCCACATAATCAC+TGG - chr1.2:2148900-2148919 None:intergenic 40.0%
AGTTTAGTGAGACTGTTTGC+AGG - chr1.2:2149731-2149750 None:intergenic 40.0%
ATTGTACGTAACGGCATCAA+AGG - chr1.2:2150747-2150766 None:intergenic 40.0%
CATAAGCACTTATGCTCGTT+TGG + chr1.2:2149338-2149357 MS.gene27293:intron 40.0%
CATCCAAACAGAGAAAAGAC+AGG - chr1.2:2149113-2149132 None:intergenic 40.0%
CATTGAGAAGAACCTCAATC+CGG + chr1.2:2151366-2151385 MS.gene27293:CDS 40.0%
CCGCATAGATTTGATGAACA+AGG + chr1.2:2150565-2150584 MS.gene27293:CDS 40.0%
CTTAGTTTCATATGCCCTCT+AGG - chr1.2:2149664-2149683 None:intergenic 40.0%
CTTGATCATGCAAAAGAAGC+AGG + chr1.2:2151217-2151236 MS.gene27293:CDS 40.0%
GAATGTTTGAGAGAGCTTGT+AGG + chr1.2:2149171-2149190 MS.gene27293:intron 40.0%
GCTTCATAGTATATAAGCGC+TGG + chr1.2:2149563-2149582 MS.gene27293:intron 40.0%
GTCATTCTTTATGCTGGGTT+GGG + chr1.2:2151094-2151113 MS.gene27293:CDS 40.0%
TCAGCGTTATAAACACGCTT+TGG + chr1.2:2149078-2149097 MS.gene27293:CDS 40.0%
TGAGAGAGAGAAATAGAGAG+CGG - chr1.2:2148698-2148717 None:intergenic 40.0%
TGGAACTTGCACTATCAAGA+TGG - chr1.2:2151012-2151031 None:intergenic 40.0%
TGTTGCAATAAAAGGTCCTC+TGG + chr1.2:2150278-2150297 MS.gene27293:intron 40.0%
TTCTCTCTCTCATCAAACGA+AGG + chr1.2:2148706-2148725 MS.gene27293:CDS 40.0%
TTGCTGAACGATGATTGTGA+TGG - chr1.2:2148853-2148872 None:intergenic 40.0%
TTGTACGTAACGGCATCAAA+GGG - chr1.2:2150746-2150765 None:intergenic 40.0%
! ATATAGTTCCTCGGCCTTTT+CGG - chr1.2:2150699-2150718 None:intergenic 40.0%
! GTGTTGTTCAGTGCTAACTA+AGG + chr1.2:2150300-2150319 MS.gene27293:intron 40.0%
! TTATGCTGGGTTGGGAAATA+AGG + chr1.2:2151102-2151121 MS.gene27293:CDS 40.0%
! TTCCCTGTCTTTTCTCTGTT+TGG + chr1.2:2149107-2149126 MS.gene27293:intron 40.0%
!!! GGACAACCTTTTGTTGTTTG+AGG - chr1.2:2150832-2150851 None:intergenic 40.0%
AAAAATGACCGAAAAGGCCG+AGG + chr1.2:2150688-2150707 MS.gene27293:CDS 45.0%
AGGCCGAGGAACTATATGAA+AGG + chr1.2:2150702-2150721 MS.gene27293:CDS 45.0%
ATATCACGGAGCTCAGAAAC+AGG - chr1.2:2149037-2149056 None:intergenic 45.0%
CAGTGAAATATACGTTGGCC+AGG - chr1.2:2150985-2151004 None:intergenic 45.0%
GAAGAAATGCGTAATGGTGC+TGG + chr1.2:2150929-2150948 MS.gene27293:CDS 45.0%
GCAGGTAGAAAAGATCCCAT+CGG + chr1.2:2150796-2150815 MS.gene27293:CDS 45.0%
GCCAACGTATATTTCACTGC+GGG + chr1.2:2150986-2151005 MS.gene27293:CDS 45.0%
GCGTTATAAACACGCTTTGG+AGG + chr1.2:2149081-2149100 MS.gene27293:CDS 45.0%
GGCCAACGTATATTTCACTG+CGG + chr1.2:2150985-2151004 MS.gene27293:CDS 45.0%
GGTCATTCTTTATGCTGGGT+TGG + chr1.2:2151093-2151112 MS.gene27293:CDS 45.0%
TCCTGCATAAGAATGGAGGA+GGG - chr1.2:2150666-2150685 None:intergenic 45.0%
TCTTTACATGTCAGTTGGGC+AGG + chr1.2:2150778-2150797 MS.gene27293:CDS 45.0%
TGAGGTCTTCAACAACCGAT+GGG - chr1.2:2150814-2150833 None:intergenic 45.0%
TGCTGTGACCAGTGATTATG+TGG + chr1.2:2148889-2148908 MS.gene27293:CDS 45.0%
TGTACGTAACGGCATCAAAG+GGG - chr1.2:2150745-2150764 None:intergenic 45.0%
TTGAGGTCTTCAACAACCGA+TGG - chr1.2:2150815-2150834 None:intergenic 45.0%
TTGCTCCTGCATAAGAATGG+AGG - chr1.2:2150670-2150689 None:intergenic 45.0%
! CAGAGGGTGATGATGATCTA+AGG + chr1.2:2148930-2148949 MS.gene27293:CDS 45.0%
! TTAGCACTGAACAACACCAG+AGG - chr1.2:2150297-2150316 None:intergenic 45.0%
!! GTGCTGGTTCTGATGAAAGT+TGG + chr1.2:2150945-2150964 MS.gene27293:CDS 45.0%
AGTCTCGCAAGTCTTTGCAG+AGG - chr1.2:2150636-2150655 None:intergenic 50.0%
CAACGTTTCAGTGTCGTGTC+TGG - chr1.2:2150113-2150132 None:intergenic 50.0%
CACTCGTCGAGACTGAGAAA+GGG + chr1.2:2151035-2151054 MS.gene27293:CDS 50.0%
CTCCTGCATAAGAATGGAGG+AGG - chr1.2:2150667-2150686 None:intergenic 50.0%
GCACAGACAGTCAGACACAT+TGG - chr1.2:2149545-2149564 None:intergenic 50.0%
GCCCGCAGTGAAATATACGT+TGG - chr1.2:2150990-2151009 None:intergenic 50.0%
GTCTCGCAAGTCTTTGCAGA+GGG - chr1.2:2150635-2150654 None:intergenic 50.0%
TCACTCGTCGAGACTGAGAA+AGG + chr1.2:2151034-2151053 MS.gene27293:CDS 50.0%
TCATCACCCTCTGCAGAGTT+CGG - chr1.2:2148923-2148942 None:intergenic 50.0%
TCGTGACGATGTCAGACACT+GGG - chr1.2:2149436-2149455 None:intergenic 50.0%
TTCGTGACGATGTCAGACAC+TGG - chr1.2:2149437-2149456 None:intergenic 50.0%
ACGTAACGGCATCAAAGGGG+AGG - chr1.2:2150742-2150761 None:intergenic 55.0%
AGAGACTCCGAACTCTGCAG+AGG + chr1.2:2148913-2148932 MS.gene27293:CDS 55.0%
CTCAGTCTCGACGAGTGAGT+TGG - chr1.2:2151032-2151051 None:intergenic 55.0%
GAGACTCCGAACTCTGCAGA+GGG + chr1.2:2148914-2148933 MS.gene27293:CDS 55.0%
GCCCTCCTCCATTCTTATGC+AGG + chr1.2:2150662-2150681 MS.gene27293:CDS 55.0%
TCGCAAGTCTTTGCAGAGGG+TGG - chr1.2:2150632-2150651 None:intergenic 55.0%
TGGACACACGACACGAACGT+TGG - chr1.2:2149525-2149544 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 2148687 2151399 2148687 ID=MS.gene27293
chr1.2 mRNA 2148687 2151399 2148687 ID=MS.gene27293.t1;Parent=MS.gene27293
chr1.2 exon 2148687 2148727 2148687 ID=MS.gene27293.t1.exon1;Parent=MS.gene27293.t1
chr1.2 CDS 2148687 2148727 2148687 ID=cds.MS.gene27293.t1;Parent=MS.gene27293.t1
chr1.2 exon 2148823 2149102 2148823 ID=MS.gene27293.t1.exon2;Parent=MS.gene27293.t1
chr1.2 CDS 2148823 2149102 2148823 ID=cds.MS.gene27293.t1;Parent=MS.gene27293.t1
chr1.2 exon 2150506 2151399 2150506 ID=MS.gene27293.t1.exon3;Parent=MS.gene27293.t1
chr1.2 CDS 2150506 2151399 2150506 ID=cds.MS.gene27293.t1;Parent=MS.gene27293.t1
Gene Sequence

>MS.gene27293

ATGTCGTACCGCTCTCTATTTCTCTCTCTCATCAAACGAAGGTAACAAAATTTTCAAGTTCCCAACTTTCTCCAATTTTCTCAACACCCAATCAGATTCAAATTATTGGGTATTTATATTTTCTTCCATTTTGCAGAAACACCAGTTTAATCAGCAATAATTACCATCACAATCATCGTTCAGCAAGATTTTTCTCTTCAGATGCTGTGACCAGTGATTATGTGGAAGAGACTCCGAACTCTGCAGAGGGTGATGATGATCTAAGGAGTAGAATCTTGAGGCTCAGACTCCCCAAAAGAAGTGCTACTAATGTTCTTCAAAAATGGGTTCTTCAAGGGAATTCTATTCCTGTTTCTGAGCTCCGTGATATTTCTAAAGAGCTTAGGAGTTCTCAGCGTTATAAACACGCTTTGGAGGTTTTTCCCTGTCTTTTCTCTGTTTGGATGACTTATTTGAACTTATCTATTGATATAAGTACTTGTGAGAATGTTTGAGAGAGCTTGTAGGAAAAACCTTATGACATGTCCATTACTCATTAGCTTATTTCAGTTAGCTCTCTAGGATAGCTTATGCAAACAGCTTATAATTTATATGAAAATAGTTAGATTAGATCTTTTGTTATAGAAATAGTTTATATATAAGCATTTATATCATAAGCACTTATGCTCGTTTGGATAAACAGCTTATAGCATAAACACTGATCATATATAAGCTCTATAACACTGACACATCTAATCGAAGGCGTGCCCAGTGTCTGACATCGTCACGAAACCGACATATTTGATAATTTTGATTGAATTATGTCATTTTCTTAAATATTATCGTTGTTAACATGCCAACGTTCGTGTCGTGTGTCCAATGTGTCTGACTGTCTGTGCTTCATAGTATATAAGCGCTGGTTATAAGCTATTTCTATAACAAAAGATAAAATAAAGTCGATTTGTTTTCATATAATCTATAAGTTGTTTTCATATCCTAGAGGGCATATGAAACTAAGCTGAAAATGGCTTAGGAACACGTCATAAGCTGTTTTCATAAATTCCTGCAAACAGTCTCACTAAACTTATGCCGTAGTTAAGCTCAAATAAGCCAATACAGACATGTCCATATTCCATAAGTGCTTAATTGAGCTGTTTATTATTCAAACATGATCTTTTCATTAGTTCTCTGATTATAAACGAGTTCTGAAGTTTATTTGGGGTGTTATAGTTCTCTGATTGTGAATTAGGGATAATTTCTGGAAATGGTTAAAATAAAGTAAAAAAAGAATATCTTTAGAAGACATGAATTAGTAGCAGATCATTTAAGTTCTTGAATCTCTTTTGCTTTTAAGATGATGTTCTTATCCTTGTATTAGTAATCAAGACAATTACTAAATCTTCATCAATGATTATTAATAGCTAATGAGGCACGGACACAGACACCAGACACGACACTGAAACGTTGACACCAAATATTATTTGAGAAAATGACTTAATTGAATGTAATGTCATGAATCGTTGTCTTGTCAGTGTTGGACACTGCAACAGAGATTAATAGTTCTGTAAGGTTCACAAATGAATAGTTTACTTAGTTTTGTAATACTTGTAACTGTTGCAATAAAAGGTCCTCTGGTGTTGTTCAGTGCTAACTAAGGGTCTATGTTAAATCTGAGTGCATGTTAGGCCATTGCAACGCCAAAGAAGTTTCCAGAAGTGAATATTATTTTGAATTAATATTGTTCGGCTATTCATGAGTGTTATTCTGATTCATTGTAGTTTCATGTATTAGTTTTGCTCTATAAAACGGCTTCACAATTTTTTACTGTTGATGATTTCAGATATCAGAATGGATGGTTAGTCATGAAGAATATGAGTTATCGGACTCTGATTATGCAGACCGCATAGATTTGATGAACAAGGTTTTTGGCATTGATGCTGCAGAGCGCTACTTTGAAGCTTTACCACCCTCTGCAAAGACTTGCGAGACTTACACGGCCCTCCTCCATTCTTATGCAGGAGCAAAAATGACCGAAAAGGCCGAGGAACTATATGAAAGGATAAAAGATTCAAACCTCCCCTTTGATGCCGTTACGTACAATGAAATGATGACTCTTTACATGTCAGTTGGGCAGGTAGAAAAGATCCCATCGGTTGTTGAAGACCTCAAACAACAAAAGGTTGTCCCTGATATCTTTACTTTTAATCTATGGATAAGTTCATGTGCTGCTTCTCTTAATATTGATGAAGTTAGAAGGATTCTTGAAGAAATGCGTAATGGTGCTGGTTCTGATGAAAGTTGGATTAGATATTTGAACCTGGCCAACGTATATTTCACTGCGGGCCATCTTGATAGTGCAAGTTCCAACTCACTCGTCGAGACTGAGAAAGGGATCACACAAAGTCAATGGATAACATATGACTTACTGGTCATTCTTTATGCTGGGTTGGGAAATAAGGATAAACTTGATCAAATATGGAATTCATTGAGAATGACTAAACAGAAAATGATTAGCAGGAATTATATCTGCATTATTTCTGCATATCTGATTCTTGATCATGCAAAAGAAGCAGGTGAAGTTATTGATCAATGGAAGCAATCGACTACCACAGATTTTGATGTGCTTGATTGTAAAAGGATTATGGATTCTTTTACGGAAATTGGATTGGATGAAGTAGCCAACAACTTGAATATGATTCTCATTGAGAAGAACCTCAATCCGGAAAATAACTAA
Protein sequence

>MS.gene27293.t1

MSYRSLFLSLIKRRNTSLISNNYHHNHRSARFFSSDAVTSDYVEETPNSAEGDDDLRSRILRLRLPKRSATNVLQKWVLQGNSIPVSELRDISKELRSSQRYKHALEISEWMVSHEEYELSDSDYADRIDLMNKVFGIDAAERYFEALPPSAKTCETYTALLHSYAGAKMTEKAEELYERIKDSNLPFDAVTYNEMMTLYMSVGQVEKIPSVVEDLKQQKVVPDIFTFNLWISSCAASLNIDEVRRILEEMRNGAGSDESWIRYLNLANVYFTAGHLDSASSNSLVETEKGITQSQWITYDLLVILYAGLGNKDKLDQIWNSLRMTKQKMISRNYICIISAYLILDHAKEAGEVIDQWKQSTTTDFDVLDCKRIMDSFTEIGLDEVANNLNMILIEKNLNPENN