AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27356

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27356.t1 MTR_1g021740 98.357 487 8 0 1 487 1 487 0.0 952
MS.gene27356.t1 MTR_8g069470 56.984 451 194 0 36 486 51 501 0.0 528
MS.gene27356.t1 MTR_5g033100 54.244 483 212 2 8 481 20 502 8.06e-179 511
MS.gene27356.t1 MTR_1g100180 49.798 496 239 4 1 487 1 495 6.65e-168 483
MS.gene27356.t1 MTR_5g032720 49.779 452 226 1 29 479 30 481 1.73e-157 456
MS.gene27356.t1 MTR_3g099700 50.862 464 227 1 20 482 31 494 3.41e-156 453
MS.gene27356.t1 MTR_2g088400 49.184 490 233 3 4 479 10 497 1.27e-155 452
MS.gene27356.t1 MTR_2g022980 48.354 486 242 3 3 479 8 493 2.15e-153 446
MS.gene27356.t1 MTR_4g048000 47.305 501 242 4 3 481 11 511 8.81e-151 440
MS.gene27356.t1 MTR_5g032750 49.160 476 232 3 6 480 16 482 3.26e-150 437
MS.gene27356.t1 MTR_7g087410 47.289 461 243 0 27 487 23 483 6.78e-149 434
MS.gene27356.t1 MTR_7g087400 46.957 460 241 2 21 480 18 474 4.49e-147 429
MS.gene27356.t1 MTR_3g099720 44.325 467 260 0 12 478 18 484 3.59e-142 417
MS.gene27356.t1 MTR_2g088400 48.667 450 215 3 4 439 10 457 1.36e-138 407
MS.gene27356.t1 MTR_6g027200 44.906 481 258 4 1 480 7 481 1.20e-137 406
MS.gene27356.t1 MTR_6g027190 48.261 460 234 3 22 480 13 469 4.63e-137 404
MS.gene27356.t1 MTR_7g087410 48.985 394 201 0 94 487 1 394 2.04e-132 389
MS.gene27356.t1 MTR_6g047610 41.929 477 273 3 3 479 1 473 1.16e-131 390
MS.gene27356.t1 MTR_7g087370 45.098 459 220 3 21 479 18 444 1.62e-130 386
MS.gene27356.t1 MTR_6g047640 42.348 477 272 2 3 479 1 474 2.00e-129 384
MS.gene27356.t1 MTR_6g047590 42.348 477 271 3 3 479 1 473 1.45e-125 374
MS.gene27356.t1 MTR_7g087430 46.891 386 205 0 94 479 1 386 8.07e-125 370
MS.gene27356.t1 MTR_5g090290 45.416 469 252 2 14 478 22 490 1.60e-123 372
MS.gene27356.t1 MTR_6g047640 40.252 477 263 3 3 479 1 455 6.00e-122 365
MS.gene27356.t1 MTR_8g063590 43.584 452 253 2 36 485 54 505 7.09e-114 345
MS.gene27356.t1 MTR_1g108810 39.186 467 274 5 12 470 6 470 8.14e-103 316
MS.gene27356.t1 MTR_6g081410 38.095 462 279 4 20 478 14 471 2.75e-100 310
MS.gene27356.t1 MTR_6g081780 39.224 464 267 6 20 476 14 469 8.57e-99 306
MS.gene27356.t1 MTR_5g087930 43.500 400 204 4 61 459 52 430 1.84e-96 299
MS.gene27356.t1 MTR_7g082810 38.235 442 272 1 36 476 21 462 1.41e-93 292
MS.gene27356.t1 MTR_6g066380 36.952 479 291 4 1 476 1 471 2.36e-93 291
MS.gene27356.t1 MTR_6g081300 37.862 449 272 3 36 480 11 456 1.02e-90 285
MS.gene27356.t1 MTR_6g081810 35.504 476 274 5 1 476 1 443 4.23e-87 275
MS.gene27356.t1 MTR_6g081390 38.031 447 268 5 37 478 15 457 6.96e-87 275
MS.gene27356.t1 MTR_1g108840 35.404 483 296 7 3 476 8 483 2.98e-85 271
MS.gene27356.t1 MTR_6g081240 37.220 446 274 5 27 468 1 444 1.08e-84 268
MS.gene27356.t1 MTR_8g106670 37.020 443 277 2 37 478 15 456 4.07e-84 268
MS.gene27356.t1 MTR_6g081400 35.938 448 276 5 37 478 18 460 1.09e-82 264
MS.gene27356.t1 MTR_6g004220 34.802 454 292 3 36 487 24 475 2.90e-81 260
MS.gene27356.t1 MTR_6g081310 38.427 445 255 6 48 476 9 450 3.01e-81 260
MS.gene27356.t1 MTR_1g108990 36.425 442 280 1 36 476 26 467 2.45e-80 258
MS.gene27356.t1 MTR_6g004220 34.943 435 281 2 36 468 31 465 5.27e-78 252
MS.gene27356.t1 MTR_2g078930 34.110 472 297 5 4 467 11 476 3.89e-77 250
MS.gene27356.t1 MTR_1g109060 34.842 442 287 1 36 476 25 466 2.99e-75 244
MS.gene27356.t1 MTR_2g078960 35.104 433 280 1 36 467 34 466 2.07e-74 243
MS.gene27356.t1 MTR_2g079020 33.871 434 284 2 36 467 38 470 8.45e-74 241
MS.gene27356.t1 MTR_2g078970 35.566 433 278 1 36 467 32 464 8.73e-74 241
MS.gene27356.t1 MTR_5g090280 37.530 413 223 4 94 478 1 406 2.42e-73 238
MS.gene27356.t1 MTR_1g109060 34.793 434 282 1 36 468 25 458 9.18e-73 238
MS.gene27356.t1 MTR_7g082800 34.197 386 253 1 94 478 1 386 3.28e-70 229
MS.gene27356.t1 MTR_5g010830 30.562 445 296 5 36 473 66 504 8.57e-62 211
MS.gene27356.t1 MTR_3g111020 28.814 472 327 4 12 477 26 494 1.90e-60 207
MS.gene27356.t1 MTR_1g101500 29.536 474 321 5 6 474 39 504 1.32e-59 205
MS.gene27356.t1 MTR_0039s0080 28.571 490 334 5 1 482 1 482 4.17e-59 203
MS.gene27356.t1 MTR_5g067460 28.125 448 311 4 36 477 56 498 1.06e-57 200
MS.gene27356.t1 MTR_6g004220 31.880 367 248 2 36 400 31 397 2.15e-50 178
MS.gene27356.t1 MTR_2g078990 35.052 291 188 1 178 467 3 293 1.17e-47 167
MS.gene27356.t1 MTR_6g088805 26.237 465 334 4 10 473 7 463 2.21e-47 171
MS.gene27356.t1 MTR_6g043810 27.494 451 313 5 36 474 24 472 1.68e-46 169
MS.gene27356.t1 MTR_6g043810 27.494 451 313 5 36 474 34 482 2.61e-46 169
MS.gene27356.t1 MTR_5g011540 27.880 434 311 1 42 473 50 483 2.83e-43 160
MS.gene27356.t1 MTR_8g102430 26.651 439 301 3 36 473 24 442 1.92e-42 157
MS.gene27356.t1 MTR_4g027920 25.652 460 319 5 26 472 147 596 1.24e-41 157
MS.gene27356.t1 MTR_6g004220 32.374 278 186 2 36 311 31 308 7.45e-38 141
MS.gene27356.t1 MTR_2g435930 23.109 476 349 3 18 476 2 477 1.82e-33 132
MS.gene27356.t1 MTR_2g079030 36.923 195 123 0 273 467 319 513 2.94e-33 132
MS.gene27356.t1 MTR_4g131290 50.820 61 30 0 107 167 13 73 3.82e-15 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27356.t1 AT1G47530 67.925 477 150 2 6 482 8 481 0.0 657
MS.gene27356.t1 AT1G47530 71.465 389 111 0 94 482 1 389 0.0 572
MS.gene27356.t1 AT3G26590 54.725 455 206 0 30 484 39 493 0.0 517
MS.gene27356.t1 AT5G38030 53.347 478 217 2 13 484 16 493 1.11e-178 511
MS.gene27356.t1 AT1G23300 56.098 451 198 0 30 480 38 488 1.72e-177 508
MS.gene27356.t1 AT1G12950 54.646 452 205 0 30 481 61 512 3.15e-176 505
MS.gene27356.t1 AT4G00350 55.157 446 199 1 35 480 87 531 2.74e-172 496
MS.gene27356.t1 AT4G25640 51.782 477 221 3 14 482 4 479 1.07e-158 459
MS.gene27356.t1 AT4G25640 51.782 477 221 3 14 482 4 479 4.73e-158 459
MS.gene27356.t1 AT3G21690 49.149 470 236 2 13 479 27 496 6.87e-151 440
MS.gene27356.t1 AT3G26590 53.708 391 181 0 94 484 1 391 2.30e-150 435
MS.gene27356.t1 AT5G65380 48.712 466 238 1 20 484 21 486 7.22e-150 437
MS.gene27356.t1 AT1G61890 45.963 483 255 3 3 479 9 491 2.65e-146 428
MS.gene27356.t1 AT1G11670 47.368 456 239 1 28 482 42 497 4.72e-144 422
MS.gene27356.t1 AT4G25640 51.852 432 199 3 14 437 4 434 1.99e-143 419
MS.gene27356.t1 AT1G61890 45.992 474 250 3 3 470 9 482 3.44e-142 417
MS.gene27356.t1 AT1G33100 46.405 459 246 0 21 479 18 476 1.74e-141 416
MS.gene27356.t1 AT1G33110 46.070 458 247 0 22 479 22 479 5.73e-141 414
MS.gene27356.t1 AT5G44050 47.379 477 238 5 6 479 18 484 8.66e-141 414
MS.gene27356.t1 AT1G33090 46.507 458 245 0 22 479 22 479 4.40e-138 407
MS.gene27356.t1 AT5G10420 47.761 469 238 5 15 479 17 482 2.10e-137 405
MS.gene27356.t1 AT1G33080 44.978 458 252 0 22 479 22 479 1.24e-135 400
MS.gene27356.t1 AT4G21910 45.254 453 246 2 28 479 47 498 2.06e-134 398
MS.gene27356.t1 AT4G21910 45.254 453 246 2 28 479 47 498 2.06e-134 398
MS.gene27356.t1 AT4G21910 44.828 464 252 3 19 479 38 500 3.99e-134 397
MS.gene27356.t1 AT4G21910 44.828 464 252 3 19 479 38 500 3.99e-134 397
MS.gene27356.t1 AT3G59030 47.404 443 232 1 36 477 51 493 2.50e-133 395
MS.gene27356.t1 AT1G33080 45.133 452 248 0 22 473 22 473 6.85e-133 394
MS.gene27356.t1 AT4G21903 42.683 492 274 4 3 487 14 504 1.64e-129 385
MS.gene27356.t1 AT1G61890 44.921 443 238 3 3 439 9 451 9.94e-127 377
MS.gene27356.t1 AT4G21903 43.064 483 267 4 3 478 14 495 2.09e-126 378
MS.gene27356.t1 AT4G21903 45.570 395 214 1 94 487 1 395 1.45e-123 366
MS.gene27356.t1 AT5G17700 39.651 459 277 0 21 479 19 477 1.75e-116 352
MS.gene27356.t1 AT5G17700 39.651 459 277 0 21 479 19 477 1.75e-116 352
MS.gene27356.t1 AT3G03620 39.783 460 277 0 20 479 21 480 2.76e-115 349
MS.gene27356.t1 AT3G03620 39.783 460 277 0 20 479 21 480 2.76e-115 349
MS.gene27356.t1 AT1G33110 46.335 382 205 0 22 403 22 403 1.81e-111 336
MS.gene27356.t1 AT3G59030 46.175 366 196 1 36 400 51 416 1.94e-100 308
MS.gene27356.t1 AT1G73700 40.899 467 265 6 14 476 6 465 1.40e-98 305
MS.gene27356.t1 AT5G52450 41.176 442 258 2 38 478 29 469 3.19e-97 302
MS.gene27356.t1 AT2G34360 39.046 461 263 8 22 477 16 463 3.30e-89 281
MS.gene27356.t1 AT1G71140 36.771 446 273 3 36 476 26 467 1.10e-86 275
MS.gene27356.t1 AT5G17700 37.859 383 238 0 21 403 19 401 2.03e-85 269
MS.gene27356.t1 AT1G66760 32.359 479 307 5 2 476 4 469 1.73e-83 266
MS.gene27356.t1 AT3G23560 34.874 476 291 7 2 467 5 471 3.55e-82 263
MS.gene27356.t1 AT1G66760 32.272 471 302 5 2 468 4 461 4.00e-81 259
MS.gene27356.t1 AT2G04080 32.823 457 300 2 32 487 23 473 4.50e-80 257
MS.gene27356.t1 AT1G66780 35.129 427 272 2 53 476 52 476 4.53e-80 257
MS.gene27356.t1 AT1G15160 35.794 447 276 4 36 476 31 472 1.30e-79 256
MS.gene27356.t1 AT1G15150 35.281 445 281 4 36 476 31 472 1.56e-78 253
MS.gene27356.t1 AT2G04040 32.649 487 312 5 3 487 1 473 1.58e-78 253
MS.gene27356.t1 AT1G64820 33.781 447 281 5 38 476 31 470 4.00e-78 253
MS.gene27356.t1 AT2G04070 33.403 476 307 3 16 487 4 473 2.67e-77 250
MS.gene27356.t1 AT2G34360 35.870 460 237 6 22 477 16 421 3.28e-77 248
MS.gene27356.t1 AT2G04050 33.333 465 306 2 16 476 4 468 4.23e-76 247
MS.gene27356.t1 AT2G04080 32.648 438 294 1 32 468 23 460 5.22e-76 246
MS.gene27356.t1 AT1G15170 35.011 457 280 7 35 482 33 481 2.47e-75 245
MS.gene27356.t1 AT3G23550 33.623 461 299 4 14 467 3 463 5.70e-75 244
MS.gene27356.t1 AT2G04100 32.353 442 298 1 36 476 30 471 6.90e-72 236
MS.gene27356.t1 AT2G04090 31.448 442 302 1 36 476 30 471 1.73e-70 232
MS.gene27356.t1 AT1G15180 35.088 456 279 7 36 482 35 482 5.59e-70 231
MS.gene27356.t1 AT2G04100 32.110 436 295 1 36 470 30 465 2.62e-69 229
MS.gene27356.t1 AT1G15150 36.499 337 211 2 142 476 21 356 1.09e-65 216
MS.gene27356.t1 AT1G15170 36.311 347 210 4 142 482 21 362 3.83e-64 212
MS.gene27356.t1 AT1G58340 29.933 451 307 3 36 481 58 504 2.10e-60 207
MS.gene27356.t1 AT3G23560 33.252 409 254 7 2 400 5 404 5.42e-60 203
MS.gene27356.t1 AT1G66760 32.931 331 215 3 142 468 6 333 1.31e-58 197
MS.gene27356.t1 AT5G19700 26.907 472 332 3 16 474 4 475 1.58e-54 191
MS.gene27356.t1 AT1G66760 29.529 403 267 5 2 400 4 393 1.80e-53 185
MS.gene27356.t1 AT4G29140 26.609 466 341 1 10 474 30 495 1.92e-51 183
MS.gene27356.t1 AT2G38510 26.222 450 320 5 32 474 2 446 6.74e-47 169
MS.gene27356.t1 AT1G71870 25.103 486 336 6 8 472 1 479 6.52e-46 167
MS.gene27356.t1 AT4G23030 29.122 467 318 6 20 478 15 476 6.05e-45 164
MS.gene27356.t1 AT5G52050 25.942 451 315 2 36 473 35 479 7.21e-42 156
MS.gene27356.t1 AT5G49130 24.536 485 340 5 18 478 1 483 1.62e-40 152
MS.gene27356.t1 AT1G15180 35.094 265 161 4 142 400 21 280 6.73e-39 146
MS.gene27356.t1 AT4G22790 26.106 452 319 7 29 473 27 470 9.63e-36 139
MS.gene27356.t1 AT2G04066 38.843 121 74 0 356 476 43 163 2.39e-23 97.1

Find 115 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTAATATTGAAACTTAAT+TGG 0.102187 1.2:+2880322 MS.gene27356:CDS
TGATTTGTTATCAGGGTATT+TGG 0.215741 1.2:+2881772 MS.gene27356:intron
TGGTTTCGCCTTTGGTTTCT+TGG 0.217826 1.2:+2878586 MS.gene27356:CDS
TTGGGCTTCACATTTGGTTT+TGG 0.259862 1.2:+2881562 MS.gene27356:CDS
TCTAATATTGAAACTTAATT+GGG 0.293407 1.2:+2880323 MS.gene27356:CDS
TTAATGAAGTAACAATTATA+AGG 0.295058 1.2:-2881833 None:intergenic
GTTGAAAATTCTGTTATTGC+TGG 0.307891 1.2:+2878566 MS.gene27356:CDS
GTTATTGCTGGTTTCGCCTT+TGG 0.310201 1.2:+2878578 MS.gene27356:CDS
CATTGGATGCTGTATCTATT+TGG 0.321661 1.2:+2880679 MS.gene27356:CDS
CAGCAAGTTTAGTTGTTTCC+TGG 0.333817 1.2:-2881298 None:intergenic
CTTGTAGCTTACATAAATCT+TGG 0.340357 1.2:+2881502 MS.gene27356:CDS
TAGCTTCAGCTGTTATGTTA+TGG 0.343938 1.2:+2880499 MS.gene27356:CDS
GTGATGGCAGCTTGGAGTTT+TGG 0.345028 1.2:+2880604 MS.gene27356:intron
CAGAAAGCCGTGTGAGGAAG+TGG 0.348907 1.2:+2881965 MS.gene27356:CDS
ATGATATTGGTGGTGATAAC+AGG 0.354451 1.2:+2880633 MS.gene27356:CDS
ATATTGTTGGGCTTCACATT+TGG 0.357452 1.2:+2881556 MS.gene27356:CDS
GATAAAGAAGTTAAGATTGT+TGG 0.357514 1.2:-2878470 None:intergenic
CTTGGATCCTACTATGTTGT+TGG 0.361711 1.2:+2881520 MS.gene27356:CDS
TTACCAGCTGGGATATTGTT+GGG 0.365810 1.2:+2881544 MS.gene27356:CDS
TGGTTGGCATCAGTTGGGTA+TGG 0.366331 1.2:+2878845 MS.gene27356:intron
AGTAACAATTATAAGGATTA+TGG 0.368933 1.2:-2881826 None:intergenic
GATTGCAGTATGAACATAAA+TGG 0.371621 1.2:+2880939 MS.gene27356:intron
GGAGTTGCCCAAATATAAAA+AGG 0.371768 1.2:-2878984 None:intergenic
TTGTTACTTCATTAACCAAC+TGG 0.380634 1.2:+2881841 MS.gene27356:CDS
AAACTTAATTGGGGCTTAGT+TGG 0.382505 1.2:+2880333 MS.gene27356:CDS
GGAAAGTCCTGGTGATGTTA+TGG 0.383348 1.2:+2880256 MS.gene27356:CDS
GGTGGTGATAACAGGGCATT+TGG 0.388031 1.2:+2880641 MS.gene27356:CDS
ACTCCAAGCACCATCTGATT+TGG 0.390160 1.2:-2880424 None:intergenic
TGTTATCAGGGTATTTGGTC+AGG 0.396483 1.2:+2881777 MS.gene27356:intron
GGTATTTGGTCAGGTCTGAT+TGG 0.397825 1.2:+2881786 MS.gene27356:CDS
ATTACCAGCTGGGATATTGT+TGG 0.407188 1.2:+2881543 MS.gene27356:CDS
GCATTCACTTCTACGTTTGT+TGG 0.412634 1.2:+2878509 MS.gene27356:CDS
TGGTAGGCACATTTGCTTTG+TGG 0.414802 1.2:+2880169 MS.gene27356:intron
AGGGCATTTGGCAAACCCTT+TGG 0.415152 1.2:+2880653 MS.gene27356:CDS
AAAGCATATGCAAATATTTG+TGG 0.422937 1.2:-2880198 None:intergenic
CTGAGAATTGTTCACAAAGT+TGG 0.426264 1.2:+2878413 MS.gene27356:CDS
ACACTATGTGGGCAAGCATT+TGG 0.433648 1.2:+2878882 MS.gene27356:CDS
TGGATTATCTTGGTGGCTAC+AGG 0.437574 1.2:+2878948 MS.gene27356:CDS
TTAGGTGTTGCTATTGGAGC+TGG 0.443556 1.2:+2881469 MS.gene27356:intron
GGCAAACCCTTTGGTGCCAT+TGG 0.443751 1.2:+2880662 MS.gene27356:CDS
TAGGGTTTAACGTTGCAATA+AGG 0.443944 1.2:+2880982 MS.gene27356:CDS
TGTTACATGTACCTCTTAGT+TGG 0.444285 1.2:+2880298 MS.gene27356:CDS
GAATTTACATGCAAAGATCA+TGG 0.447765 1.2:+2878928 MS.gene27356:CDS
AGCAAGTTTAGTTGTTTCCT+GGG 0.447936 1.2:-2881297 None:intergenic
TATGTTGTTGGATTACCAGC+TGG 0.453772 1.2:+2881532 MS.gene27356:CDS
TGCAAAGTTCTCAGTGATAG+TGG 0.455040 1.2:+2881175 MS.gene27356:CDS
GCAGTATGAACATAAATGGT+TGG 0.464973 1.2:+2880943 MS.gene27356:intron
GGTTGGCATCAGTTGGGTAT+GGG 0.465741 1.2:+2878846 MS.gene27356:intron
CAGTATGAACATAAATGGTT+GGG 0.468025 1.2:+2880944 MS.gene27356:intron
TCATGGTGGTTGATTGTCAT+TGG 0.473305 1.2:+2880378 MS.gene27356:CDS
GCAAAGATCATGGATTATCT+TGG 0.476538 1.2:+2878938 MS.gene27356:CDS
AGCCTACAACCAGTCTTGTC+TGG 0.477429 1.2:+2881350 MS.gene27356:CDS
TGGGATATGATGATCGCGCT+AGG 0.486272 1.2:+2880963 MS.gene27356:CDS
TGGTAATCCAACAACATAGT+AGG 0.486946 1.2:-2881527 None:intergenic
AGAAGTTAAGATTGTTGGAC+CGG 0.486994 1.2:-2878465 None:intergenic
AATATTAGAAGCCAACTAAG+AGG 0.488197 1.2:-2880309 None:intergenic
CAATTATTCTCAACACATCA+TGG 0.498659 1.2:+2880361 MS.gene27356:CDS
ATTTGGTCAGGTCTGATTGG+AGG 0.508178 1.2:+2881789 MS.gene27356:CDS
TCACCAAATCAGATGGTGCT+TGG 0.510768 1.2:+2880421 MS.gene27356:CDS
TCTTTATCTCAGTACTCGCT+TGG 0.519020 1.2:+2878485 MS.gene27356:CDS
GTGATGTTATGGATATCATT+AGG 0.521877 1.2:+2880267 MS.gene27356:CDS
GTTGGGTATGGGAAGTGCAT+TGG 0.523324 1.2:+2878857 MS.gene27356:intron
AGTTGTTAGTACTATGATCA+TGG 0.530879 1.2:-2881231 None:intergenic
GATGAGATCGCAGAAGCTGC+TGG 0.531826 1.2:+2879032 MS.gene27356:CDS
TTCCTCCCCACTTCCTCACA+CGG 0.534562 1.2:-2881972 None:intergenic
TGCTGGTCATATAAGAATGA+TGG 0.538828 1.2:+2878905 MS.gene27356:CDS
TAGCTTGAAGTGAACAATTG+AGG 0.540499 1.2:-2881266 None:intergenic
GAGTGGAGTCGAAGAAGCTA+TGG 0.541517 1.2:+2878435 MS.gene27356:CDS
TTCAAGCTATGAGGTTGCCC+AGG 0.542260 1.2:+2881280 MS.gene27356:CDS
ACAGCATCCAATGGCACCAA+AGG 0.544225 1.2:-2880669 None:intergenic
AACCAGACAAGACTGGTTGT+AGG 0.545104 1.2:-2881352 None:intergenic
ATTGTTCACTTCAAGCTATG+AGG 0.547129 1.2:+2881271 MS.gene27356:CDS
CAAGATCGAGCTCGTTAACA+TGG 0.548496 1.2:-2878532 None:intergenic
TCTCTCACATCAATATCCAT+TGG 0.549511 1.2:+2881200 MS.gene27356:CDS
TGTGACTAACCAGACAAGAC+TGG 0.551439 1.2:-2881359 None:intergenic
GAAAGCCGTGTGAGGAAGTG+GGG 0.552917 1.2:+2881967 MS.gene27356:CDS
ACTCCCCTCTTGAAGCTCGT+TGG 0.555491 1.2:+2879002 MS.gene27356:CDS
AGAAAGCCGTGTGAGGAAGT+GGG 0.557799 1.2:+2881966 MS.gene27356:CDS
AAGATCATGGATTATCTTGG+TGG 0.557828 1.2:+2878941 MS.gene27356:CDS
GTATCAAATGAACTTGGAGC+AGG 0.561306 1.2:+2881140 MS.gene27356:CDS
AAGCCCAACAATATCCCAGC+TGG 0.562621 1.2:-2881547 None:intergenic
GTGCTTGGAGTGGATTCTCA+TGG 0.564381 1.2:+2880436 MS.gene27356:CDS
ATGTTGTTGGATTACCAGCT+GGG 0.564636 1.2:+2881533 MS.gene27356:CDS
GTGAGAGTATCAAATGAACT+TGG 0.567071 1.2:+2881134 MS.gene27356:CDS
TTGACATTGTTATCTAGTAG+AGG 0.570811 1.2:-2878350 None:intergenic
CTAATATTGAAACTTAATTG+GGG 0.574308 1.2:+2880324 MS.gene27356:CDS
ACAAGCACAAAGGAAAGTCC+TGG 0.574888 1.2:+2880245 MS.gene27356:CDS
AAATCAGATGGTGCTTGGAG+TGG 0.576701 1.2:+2880426 MS.gene27356:CDS
GGGATATGATGATCGCGCTA+GGG 0.580089 1.2:+2880964 MS.gene27356:CDS
TGTGGGCAAGCATTTGGTGC+TGG 0.581549 1.2:+2878888 MS.gene27356:CDS
CAGCATCCAATGGCACCAAA+GGG 0.581611 1.2:-2880668 None:intergenic
TTGGTACTTGATGATATTGG+TGG 0.583882 1.2:+2880623 MS.gene27356:CDS
GATCATGGCAACAACACCAA+TGG 0.586370 1.2:-2881216 None:intergenic
AGTGCATTGGAGACACTATG+TGG 0.586848 1.2:+2878870 MS.gene27356:CDS
TCCTAGTGTTGTTCATCATG+AGG 0.592021 1.2:+2878379 MS.gene27356:CDS
AATTGTTCACAAAGTTGGAG+TGG 0.593504 1.2:+2878418 MS.gene27356:CDS
AAGAAGCTATGGAAAATAGC+CGG 0.605436 1.2:+2878446 MS.gene27356:CDS
CTGTCCAACGAGCTTCAAGA+GGG 0.606132 1.2:-2879006 None:intergenic
GCTGGTCATATAAGAATGAT+GGG 0.606977 1.2:+2878906 MS.gene27356:CDS
TCCTCATGATGAACAACACT+AGG 0.609928 1.2:-2878380 None:intergenic
TAGAGGACTGTCGATAGCCA+TGG 0.613803 1.2:-2878333 None:intergenic
ATGATATCCATAACATCACC+AGG 0.617590 1.2:-2880263 None:intergenic
CAAATAGATACAGCATCCAA+TGG 0.632386 1.2:-2880678 None:intergenic
TCTGTCCAACGAGCTTCAAG+AGG 0.635922 1.2:-2879007 None:intergenic
AAAAGTTCTTACAAGCACAA+AGG 0.640238 1.2:+2880235 MS.gene27356:CDS
TTTACATACCAAGAAACCAA+AGG 0.654334 1.2:-2878594 None:intergenic
ATGAAGCAGAAAGCCGTGTG+AGG 0.654673 1.2:+2881959 MS.gene27356:CDS
TCATGATGAACAACACTAGG+AGG 0.658187 1.2:-2878377 None:intergenic
GTGTTGCTATTGGAGCTGGT+TGG 0.658329 1.2:+2881473 MS.gene27356:CDS
GTGCATTGGAGACACTATGT+GGG 0.665609 1.2:+2878871 MS.gene27356:CDS
ACTTTGTGAACAATTCTCAG+TGG 0.666761 1.2:-2878410 None:intergenic
TGATATTGGTGGTGATAACA+GGG 0.675304 1.2:+2880634 MS.gene27356:CDS
AGCCGTGTGAGGAAGTGGGG+AGG 0.676849 1.2:+2881970 MS.gene27356:CDS
TGTCCAACGAGCTTCAAGAG+GGG 0.697789 1.2:-2879005 None:intergenic
TTATTCTCAACACATCATGG+TGG 0.744685 1.2:+2880364 MS.gene27356:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATACATTTTTTTTTTTTTT+TGG - chr1.2:2878790-2878809 None:intergenic 10.0%
!!! TATAAAATTGTTTTTGTTTT+TGG + chr1.2:2878825-2878844 MS.gene27356:intron 10.0%
!!! TATATAGTATAATTTTTAGA+AGG + chr1.2:2881643-2881662 MS.gene27356:intron 10.0%
!! AATTAAATTCAAGAGAAATA+AGG - chr1.2:2881608-2881627 None:intergenic 15.0%
!! ATATTCCTATCATTTAAATT+TGG + chr1.2:2879074-2879093 MS.gene27356:intron 15.0%
!! ATTAAATTCAAGAGAAATAA+GGG - chr1.2:2881607-2881626 None:intergenic 15.0%
!! ATTTACAAGAGAAATATTTA+CGG - chr1.2:2879128-2879147 None:intergenic 15.0%
!! GAAAATTAATTTCATATCTT+TGG + chr1.2:2881923-2881942 MS.gene27356:intron 15.0%
!! TCAACTTAATTAATATTTGA+TGG + chr1.2:2880551-2880570 MS.gene27356:intron 15.0%
!! TCTAATATTGAAACTTAATT+GGG + chr1.2:2880323-2880342 MS.gene27356:CDS 15.0%
!! TTAATGAAGTAACAATTATA+AGG - chr1.2:2881836-2881855 None:intergenic 15.0%
!! TTCTAATATTGAAACTTAAT+TGG + chr1.2:2880322-2880341 MS.gene27356:CDS 15.0%
!!! ATATTTATTGTTTGTTAATG+AGG + chr1.2:2881683-2881702 MS.gene27356:intron 15.0%
!!! ATTAATTTTCAACAAACAAT+AGG - chr1.2:2881913-2881932 None:intergenic 15.0%
!!! TATTTTATCAATTTTCCTTT+TGG + chr1.2:2880149-2880168 MS.gene27356:intron 15.0%
!!! TTTTATAAATCATAAACTTG+AGG - chr1.2:2878812-2878831 None:intergenic 15.0%
!! AGTAACAATTATAAGGATTA+TGG - chr1.2:2881829-2881848 None:intergenic 20.0%
!! AGTAAGTTAATTTAAGTAGT+TGG + chr1.2:2879426-2879445 MS.gene27356:intron 20.0%
!! AGTGTAATGTAATAATAAAG+CGG - chr1.2:2878632-2878651 None:intergenic 20.0%
!! CTAATATTGAAACTTAATTG+GGG + chr1.2:2880324-2880343 MS.gene27356:CDS 20.0%
!! TAACTTACTAACTATAATGA+TGG - chr1.2:2879415-2879434 None:intergenic 20.0%
!! TTAAATTCAAGAGAAATAAG+GGG - chr1.2:2881606-2881625 None:intergenic 20.0%
!! TTAGTCCAAATTTAAATGAT+AGG - chr1.2:2879082-2879101 None:intergenic 20.0%
!!! AAATGTCTATCACTTTTATT+TGG - chr1.2:2880752-2880771 None:intergenic 20.0%
!!! AAATTGTTTTTGTTTTTGGT+TGG + chr1.2:2878829-2878848 MS.gene27356:intron 20.0%
!!! ACACTTATTTTTGTTCTTTA+AGG + chr1.2:2878647-2878666 MS.gene27356:intron 20.0%
!!! ATTGAATTTTGAAAATGTGA+TGG + chr1.2:2880588-2880607 MS.gene27356:intron 20.0%
!!! GATATTTATTGATTCAATAG+TGG + chr1.2:2880009-2880028 MS.gene27356:intron 20.0%
!!! GTTGTTACCTTTTTATATTT+GGG + chr1.2:2878977-2878996 MS.gene27356:CDS 20.0%
!!! TAAAACTATGATAATGACTT+GGG + chr1.2:2879856-2879875 MS.gene27356:intron 20.0%
!!! TGTTGTTACCTTTTTATATT+TGG + chr1.2:2878976-2878995 MS.gene27356:CDS 20.0%
!!! TTGTTACTATTTAGTTTGAA+GGG + chr1.2:2879162-2879181 MS.gene27356:intron 20.0%
! AAAGCATATGCAAATATTTG+TGG - chr1.2:2880201-2880220 None:intergenic 25.0%
! AAAGTAGTATATTTACCCTT+TGG - chr1.2:2879621-2879640 None:intergenic 25.0%
! AAGAGAAATATTTACGGAAA+TGG - chr1.2:2879122-2879141 None:intergenic 25.0%
! AGCTTACATGTAAAAAACTT+CGG + chr1.2:2879669-2879688 MS.gene27356:intron 25.0%
! GATAAAGAAGTTAAGATTGT+TGG - chr1.2:2878473-2878492 None:intergenic 25.0%
! GATCGTTCAATGTAAATTAA+CGG + chr1.2:2879291-2879310 MS.gene27356:intron 25.0%
! TTCAAAACTGAGCATTAATT+AGG + chr1.2:2880815-2880834 MS.gene27356:intron 25.0%
!! AAAACTATGATAATGACTTG+GGG + chr1.2:2879857-2879876 MS.gene27356:intron 25.0%
!! AAAGGGATAGTTAATTTGAA+GGG + chr1.2:2879360-2879379 MS.gene27356:intron 25.0%
!! CATTTAAAATTTCCTAGCAA+GGG - chr1.2:2879764-2879783 None:intergenic 25.0%
!! GTAAAACTATGATAATGACT+TGG + chr1.2:2879855-2879874 MS.gene27356:intron 25.0%
!! TAAAGGGATAGTTAATTTGA+AGG + chr1.2:2879359-2879378 MS.gene27356:intron 25.0%
!! TAAGTTGTTCACTTTAAAGA+AGG - chr1.2:2881047-2881066 None:intergenic 25.0%
!! TTATCAATTTTCCTTTTGGT+AGG + chr1.2:2880153-2880172 MS.gene27356:intron 25.0%
!! TTGCTAGGAAATTTTAAATG+CGG + chr1.2:2879764-2879783 MS.gene27356:intron 25.0%
!!! ATACCTATGATTTGTTATCA+GGG + chr1.2:2881765-2881784 MS.gene27356:intron 25.0%
!!! CCATTTTTCGCTAATTAAAA+GGG + chr1.2:2879555-2879574 MS.gene27356:intron 25.0%
!!! CTTGTTACTATTTAGTTTGA+AGG + chr1.2:2879161-2879180 MS.gene27356:intron 25.0%
!!! GCATTTGTTGTTACTATTTT+AGG + chr1.2:2881451-2881470 MS.gene27356:intron 25.0%
!!! GGTAAATATACTACTTTTGT+GGG + chr1.2:2879624-2879643 MS.gene27356:intron 25.0%
!!! TATACCTATGATTTGTTATC+AGG + chr1.2:2881764-2881783 MS.gene27356:intron 25.0%
!!! TCCATTTTTCGCTAATTAAA+AGG + chr1.2:2879554-2879573 MS.gene27356:intron 25.0%
AAAAGTTCTTACAAGCACAA+AGG + chr1.2:2880235-2880254 MS.gene27356:CDS 30.0%
AAAGTCACAATGAAGACATA+CGG - chr1.2:2879249-2879268 None:intergenic 30.0%
AATATTAGAAGCCAACTAAG+AGG - chr1.2:2880312-2880331 None:intergenic 30.0%
ACAATGAGAATTTGGAGAAA+GGG - chr1.2:2880709-2880728 None:intergenic 30.0%
AGTTGTTAGTACTATGATCA+TGG - chr1.2:2881234-2881253 None:intergenic 30.0%
ATAAAAATTTGCAGCGTAAC+TGG + chr1.2:2879695-2879714 MS.gene27356:intron 30.0%
ATACCCTGATAACAAATCAT+AGG - chr1.2:2881771-2881790 None:intergenic 30.0%
ATTAACCAACTGGAAAAAAG+AGG + chr1.2:2881851-2881870 MS.gene27356:CDS 30.0%
CAATTATTCTCAACACATCA+TGG + chr1.2:2880361-2880380 MS.gene27356:CDS 30.0%
CAGTATGAACATAAATGGTT+GGG + chr1.2:2880944-2880963 MS.gene27356:intron 30.0%
CTTGTAGCTTACATAAATCT+TGG + chr1.2:2881502-2881521 MS.gene27356:CDS 30.0%
GAATTTACATGCAAAGATCA+TGG + chr1.2:2878928-2878947 MS.gene27356:CDS 30.0%
GATTGCAGTATGAACATAAA+TGG + chr1.2:2880939-2880958 MS.gene27356:intron 30.0%
GTGATGTTATGGATATCATT+AGG + chr1.2:2880267-2880286 MS.gene27356:CDS 30.0%
GTTGAAAATTCTGTTATTGC+TGG + chr1.2:2878566-2878585 MS.gene27356:CDS 30.0%
TCATTGTGCTATAGAAGTTA+TGG + chr1.2:2880722-2880741 MS.gene27356:intron 30.0%
TCTATAGCACAATGAGAATT+TGG - chr1.2:2880717-2880736 None:intergenic 30.0%
TTATGTTCATACTGCAATCA+TGG - chr1.2:2880938-2880957 None:intergenic 30.0%
TTCATATCTTTGGATTTCAC+AGG + chr1.2:2881933-2881952 MS.gene27356:intron 30.0%
TTGACATTGTTATCTAGTAG+AGG - chr1.2:2878353-2878372 None:intergenic 30.0%
TTTACATACCAAGAAACCAA+AGG - chr1.2:2878597-2878616 None:intergenic 30.0%
! ATTTTCATCACCAAATCAGA+TGG + chr1.2:2880414-2880433 MS.gene27356:CDS 30.0%
! CCATCTTTTCACTAGTTAAA+GGG + chr1.2:2879343-2879362 MS.gene27356:intron 30.0%
! GCATTTAAAATTTCCTAGCA+AGG - chr1.2:2879765-2879784 None:intergenic 30.0%
! GCTTGTAAGAACTTTTGAAT+TGG - chr1.2:2880231-2880250 None:intergenic 30.0%
! TAATGACCTGGTCAATTTTA+TGG - chr1.2:2880077-2880096 None:intergenic 30.0%
! TCCATCTTTTCACTAGTTAA+AGG + chr1.2:2879342-2879361 MS.gene27356:intron 30.0%
! TTGAAAAATGTTGAGTTGTG+TGG + chr1.2:2878695-2878714 MS.gene27356:intron 30.0%
! TTGTTACTTCATTAACCAAC+TGG + chr1.2:2881841-2881860 MS.gene27356:CDS 30.0%
!! CCCTTTTAATTAGCGAAAAA+TGG - chr1.2:2879558-2879577 None:intergenic 30.0%
!! GGGTAAATATACTACTTTTG+TGG + chr1.2:2879623-2879642 MS.gene27356:intron 30.0%
!! GTTTTGGTACTTGATGATAT+TGG + chr1.2:2880620-2880639 MS.gene27356:CDS 30.0%
!! TGATTTGTTATCAGGGTATT+TGG + chr1.2:2881772-2881791 MS.gene27356:intron 30.0%
!!! ACTATTTTAGGTGTTGCTAT+TGG + chr1.2:2881463-2881482 MS.gene27356:intron 30.0%
AAACTTAATTGGGGCTTAGT+TGG + chr1.2:2880333-2880352 MS.gene27356:CDS 35.0%
AACTGAGCATTAATTAGGCT+TGG + chr1.2:2880820-2880839 MS.gene27356:intron 35.0%
AAGAAGCTATGGAAAATAGC+CGG + chr1.2:2878446-2878465 MS.gene27356:CDS 35.0%
AATTGTTCACAAAGTTGGAG+TGG + chr1.2:2878418-2878437 MS.gene27356:CDS 35.0%
ACTGAGCATTAATTAGGCTT+GGG + chr1.2:2880821-2880840 MS.gene27356:intron 35.0%
ACTTTGTGAACAATTCTCAG+TGG - chr1.2:2878413-2878432 None:intergenic 35.0%
AGAAGTTAAGATTGTTGGAC+CGG - chr1.2:2878468-2878487 None:intergenic 35.0%
ATATTGTTGGGCTTCACATT+TGG + chr1.2:2881556-2881575 MS.gene27356:CDS 35.0%
ATGATATCCATAACATCACC+AGG - chr1.2:2880266-2880285 None:intergenic 35.0%
ATTGTTCACTTCAAGCTATG+AGG + chr1.2:2881271-2881290 MS.gene27356:CDS 35.0%
CAAATAGATACAGCATCCAA+TGG - chr1.2:2880681-2880700 None:intergenic 35.0%
CACAATGAGAATTTGGAGAA+AGG - chr1.2:2880710-2880729 None:intergenic 35.0%
CATTGGATGCTGTATCTATT+TGG + chr1.2:2880679-2880698 MS.gene27356:CDS 35.0%
CCCTTTAACTAGTGAAAAGA+TGG - chr1.2:2879346-2879365 None:intergenic 35.0%
CTGAGAATTGTTCACAAAGT+TGG + chr1.2:2878413-2878432 MS.gene27356:CDS 35.0%
GCAGTATGAACATAAATGGT+TGG + chr1.2:2880943-2880962 MS.gene27356:intron 35.0%
GCTGGTCATATAAGAATGAT+GGG + chr1.2:2878906-2878925 MS.gene27356:CDS 35.0%
GGAGTTGCCCAAATATAAAA+AGG - chr1.2:2878987-2879006 None:intergenic 35.0%
TAAAAGGGTAGCTAGGATTA+GGG + chr1.2:2879570-2879589 MS.gene27356:intron 35.0%
TACATTGAACGATCGACATT+GGG - chr1.2:2879285-2879304 None:intergenic 35.0%
TAGCTTCAGCTGTTATGTTA+TGG + chr1.2:2880499-2880518 MS.gene27356:CDS 35.0%
TAGCTTGAAGTGAACAATTG+AGG - chr1.2:2881269-2881288 None:intergenic 35.0%
TAGGGTTTAACGTTGCAATA+AGG + chr1.2:2880982-2881001 MS.gene27356:CDS 35.0%
TCATCCAATTGCAGTATACA+AGG + chr1.2:2880862-2880881 MS.gene27356:intron 35.0%
TCTCTCACATCAATATCCAT+TGG + chr1.2:2881200-2881219 MS.gene27356:CDS 35.0%
TGCTGGTCATATAAGAATGA+TGG + chr1.2:2878905-2878924 MS.gene27356:CDS 35.0%
TGGTAATCCAACAACATAGT+AGG - chr1.2:2881530-2881549 None:intergenic 35.0%
TGTTACATGTACCTCTTAGT+TGG + chr1.2:2880298-2880317 MS.gene27356:CDS 35.0%
TTAAAAGGGTAGCTAGGATT+AGG + chr1.2:2879569-2879588 MS.gene27356:intron 35.0%
TTACATTGAACGATCGACAT+TGG - chr1.2:2879286-2879305 None:intergenic 35.0%
TTATTCTCAACACATCATGG+TGG + chr1.2:2880364-2880383 MS.gene27356:CDS 35.0%
TTCTCCTTGTATACTGCAAT+TGG - chr1.2:2880869-2880888 None:intergenic 35.0%
! AAGATCATGGATTATCTTGG+TGG + chr1.2:2878941-2878960 MS.gene27356:CDS 35.0%
! AGCAAGTTTAGTTGTTTCCT+GGG - chr1.2:2881300-2881319 None:intergenic 35.0%
! GCAAAGATCATGGATTATCT+TGG + chr1.2:2878938-2878957 MS.gene27356:CDS 35.0%
! TAATGGCTTGTGTGTTTGTT+TGG + chr1.2:2880102-2880121 MS.gene27356:intron 35.0%
! TATGACTACAAGAGTACACA+AGG + chr1.2:2879523-2879542 MS.gene27356:intron 35.0%
! TTGGTACTTGATGATATTGG+TGG + chr1.2:2880623-2880642 MS.gene27356:CDS 35.0%
!! ATGATATTGGTGGTGATAAC+AGG + chr1.2:2880633-2880652 MS.gene27356:CDS 35.0%
!! GTGAGAGTATCAAATGAACT+TGG + chr1.2:2881134-2881153 MS.gene27356:CDS 35.0%
!! TGATATTGGTGGTGATAACA+GGG + chr1.2:2880634-2880653 MS.gene27356:CDS 35.0%
!!! ACTAACCTCTTTTTTCCAGT+TGG - chr1.2:2881859-2881878 None:intergenic 35.0%
AAAATTTCCTAGCAAGGGCA+AGG - chr1.2:2879759-2879778 None:intergenic 40.0%
AACTCAAAATGTCGATCGTC+TGG + chr1.2:2879480-2879499 MS.gene27356:intron 40.0%
AAGCAAATGTGCCTACCAAA+AGG - chr1.2:2880167-2880186 None:intergenic 40.0%
AATTGTGTGTTAGAACGCAC+GGG + chr1.2:2878718-2878737 MS.gene27356:intron 40.0%
ACCAGGTCATTACACGATAA+TGG + chr1.2:2880085-2880104 MS.gene27356:intron 40.0%
ATGTTGTTGGATTACCAGCT+GGG + chr1.2:2881533-2881552 MS.gene27356:CDS 40.0%
ATTACCAGCTGGGATATTGT+TGG + chr1.2:2881543-2881562 MS.gene27356:CDS 40.0%
CGCTAATTAAAAGGGTAGCT+AGG + chr1.2:2879563-2879582 MS.gene27356:intron 40.0%
CGGTCGAGATTATGACTATA+TGG + chr1.2:2879311-2879330 MS.gene27356:intron 40.0%
CTTGGATCCTACTATGTTGT+TGG + chr1.2:2881520-2881539 MS.gene27356:CDS 40.0%
GAATTGTGTGTTAGAACGCA+CGG + chr1.2:2878717-2878736 MS.gene27356:intron 40.0%
GCATTCACTTCTACGTTTGT+TGG + chr1.2:2878509-2878528 MS.gene27356:CDS 40.0%
GTATCAAATGAACTTGGAGC+AGG + chr1.2:2881140-2881159 MS.gene27356:CDS 40.0%
GTTTGAAGGGCTTAAATCGT+CGG + chr1.2:2879175-2879194 MS.gene27356:intron 40.0%
TATGTTGTTGGATTACCAGC+TGG + chr1.2:2881532-2881551 MS.gene27356:CDS 40.0%
TCATGATGAACAACACTAGG+AGG - chr1.2:2878380-2878399 None:intergenic 40.0%
TCATGGTGGTTGATTGTCAT+TGG + chr1.2:2880378-2880397 MS.gene27356:CDS 40.0%
TCCTCATGATGAACAACACT+AGG - chr1.2:2878383-2878402 None:intergenic 40.0%
TCTTTATCTCAGTACTCGCT+TGG + chr1.2:2878485-2878504 MS.gene27356:CDS 40.0%
TGCAAAGTTCTCAGTGATAG+TGG + chr1.2:2881175-2881194 MS.gene27356:CDS 40.0%
TGTGCACCATAAAATTGACC+AGG + chr1.2:2880068-2880087 MS.gene27356:intron 40.0%
TTACCAGCTGGGATATTGTT+GGG + chr1.2:2881544-2881563 MS.gene27356:CDS 40.0%
TTGAAAATGTGATGGCAGCT+TGG + chr1.2:2880596-2880615 MS.gene27356:intron 40.0%
! CAGCAAGTTTAGTTGTTTCC+TGG - chr1.2:2881301-2881320 None:intergenic 40.0%
! TCCTAGTGTTGTTCATCATG+AGG + chr1.2:2878379-2878398 MS.gene27356:CDS 40.0%
! TGTTATCAGGGTATTTGGTC+AGG + chr1.2:2881777-2881796 MS.gene27356:intron 40.0%
!! CACAACAATGCAAAAGTGCT+AGG - chr1.2:2880786-2880805 None:intergenic 40.0%
!! TTGGGCTTCACATTTGGTTT+TGG + chr1.2:2881562-2881581 MS.gene27356:CDS 40.0%
!!! GTTTTTGGTTGGCATCAGTT+GGG + chr1.2:2878840-2878859 MS.gene27356:intron 40.0%
!!! TGTTTTTGGTTGGCATCAGT+TGG + chr1.2:2878839-2878858 MS.gene27356:intron 40.0%
AAATCAGATGGTGCTTGGAG+TGG + chr1.2:2880426-2880445 MS.gene27356:CDS 45.0%
ACAAGCACAAAGGAAAGTCC+TGG + chr1.2:2880245-2880264 MS.gene27356:CDS 45.0%
ACACTATGTGGGCAAGCATT+TGG + chr1.2:2878882-2878901 MS.gene27356:CDS 45.0%
ACTCCAAGCACCATCTGATT+TGG - chr1.2:2880427-2880446 None:intergenic 45.0%
AGTGCATTGGAGACACTATG+TGG + chr1.2:2878870-2878889 MS.gene27356:CDS 45.0%
ATTTGGTCAGGTCTGATTGG+AGG + chr1.2:2881789-2881808 MS.gene27356:CDS 45.0%
CAAGATCGAGCTCGTTAACA+TGG - chr1.2:2878535-2878554 None:intergenic 45.0%
CCCGCTAATGTTCTCAAAGA+GGG + chr1.2:2879593-2879612 MS.gene27356:intron 45.0%
CCGCTAATGTTCTCAAAGAG+GGG + chr1.2:2879594-2879613 MS.gene27356:intron 45.0%
GATCATGGCAACAACACCAA+TGG - chr1.2:2881219-2881238 None:intergenic 45.0%
GCCATTATCGTGTAATGACC+TGG - chr1.2:2880089-2880108 None:intergenic 45.0%
GGTATTTGGTCAGGTCTGAT+TGG + chr1.2:2881786-2881805 MS.gene27356:CDS 45.0%
GTGCATTGGAGACACTATGT+GGG + chr1.2:2878871-2878890 MS.gene27356:CDS 45.0%
TCACCAAATCAGATGGTGCT+TGG + chr1.2:2880421-2880440 MS.gene27356:CDS 45.0%
TGGTAGGCACATTTGCTTTG+TGG + chr1.2:2880169-2880188 MS.gene27356:intron 45.0%
TGTGACTAACCAGACAAGAC+TGG - chr1.2:2881362-2881381 None:intergenic 45.0%
TTCTCAAAGAGGGGACCAAA+GGG + chr1.2:2879603-2879622 MS.gene27356:intron 45.0%
! AACCAGACAAGACTGGTTGT+AGG - chr1.2:2881355-2881374 None:intergenic 45.0%
! CCCCTCTTTGAGAACATTAG+CGG - chr1.2:2879597-2879616 None:intergenic 45.0%
! CCCTCTTTGAGAACATTAGC+GGG - chr1.2:2879596-2879615 None:intergenic 45.0%
! GGAAAGTCCTGGTGATGTTA+TGG + chr1.2:2880256-2880275 MS.gene27356:CDS 45.0%
! TGGATTATCTTGGTGGCTAC+AGG + chr1.2:2878948-2878967 MS.gene27356:CDS 45.0%
! TGGTTTCGCCTTTGGTTTCT+TGG + chr1.2:2878586-2878605 MS.gene27356:CDS 45.0%
!! GTTATTGCTGGTTTCGCCTT+TGG + chr1.2:2878578-2878597 MS.gene27356:CDS 45.0%
!! TTAGGTGTTGCTATTGGAGC+TGG + chr1.2:2881469-2881488 MS.gene27356:intron 45.0%
!!! CACATTTGGTTTTGGAGCAG+AGG + chr1.2:2881570-2881589 MS.gene27356:CDS 45.0%
AAGCCCAACAATATCCCAGC+TGG - chr1.2:2881550-2881569 None:intergenic 50.0%
ACAGCATCCAATGGCACCAA+AGG - chr1.2:2880672-2880691 None:intergenic 50.0%
AGAAAGCCGTGTGAGGAAGT+GGG + chr1.2:2881966-2881985 MS.gene27356:CDS 50.0%
ATGAAGCAGAAAGCCGTGTG+AGG + chr1.2:2881959-2881978 MS.gene27356:CDS 50.0%
CAGCATCCAATGGCACCAAA+GGG - chr1.2:2880671-2880690 None:intergenic 50.0%
CTGTCCAACGAGCTTCAAGA+GGG - chr1.2:2879009-2879028 None:intergenic 50.0%
GAGTGGAGTCGAAGAAGCTA+TGG + chr1.2:2878435-2878454 MS.gene27356:CDS 50.0%
GCCCGCTAATGTTCTCAAAG+AGG + chr1.2:2879592-2879611 MS.gene27356:intron 50.0%
GGGATATGATGATCGCGCTA+GGG + chr1.2:2880964-2880983 MS.gene27356:CDS 50.0%
GGTTGGCATCAGTTGGGTAT+GGG + chr1.2:2878846-2878865 MS.gene27356:intron 50.0%
GTTCTCAAAGAGGGGACCAA+AGG + chr1.2:2879602-2879621 MS.gene27356:intron 50.0%
TCTGTCCAACGAGCTTCAAG+AGG - chr1.2:2879010-2879029 None:intergenic 50.0%
TGGGATATGATGATCGCGCT+AGG + chr1.2:2880963-2880982 MS.gene27356:CDS 50.0%
TGGTTGGCATCAGTTGGGTA+TGG + chr1.2:2878845-2878864 MS.gene27356:intron 50.0%
TGTCCAACGAGCTTCAAGAG+GGG - chr1.2:2879008-2879027 None:intergenic 50.0%
TTCAAGCTATGAGGTTGCCC+AGG + chr1.2:2881280-2881299 MS.gene27356:CDS 50.0%
! AGCCTACAACCAGTCTTGTC+TGG + chr1.2:2881350-2881369 MS.gene27356:CDS 50.0%
! AGGGCATTTGGCAAACCCTT+TGG + chr1.2:2880653-2880672 MS.gene27356:CDS 50.0%
! GGTGGTGATAACAGGGCATT+TGG + chr1.2:2880641-2880660 MS.gene27356:CDS 50.0%
! GTGATGGCAGCTTGGAGTTT+TGG + chr1.2:2880604-2880623 MS.gene27356:intron 50.0%
! GTGCTTGGAGTGGATTCTCA+TGG + chr1.2:2880436-2880455 MS.gene27356:CDS 50.0%
! GTGTTGCTATTGGAGCTGGT+TGG + chr1.2:2881473-2881492 MS.gene27356:CDS 50.0%
! GTTGGGTATGGGAAGTGCAT+TGG + chr1.2:2878857-2878876 MS.gene27356:intron 50.0%
!! AAGGGCAAGGTGTTGCGATA+TGG - chr1.2:2879746-2879765 None:intergenic 50.0%
!! AGGGCAAGGTGTTGCGATAT+GGG - chr1.2:2879745-2879764 None:intergenic 50.0%
AACTGGTACCAGATCCCCCA+TGG + chr1.2:2879712-2879731 MS.gene27356:intron 55.0%
ACTCCCCTCTTGAAGCTCGT+TGG + chr1.2:2879002-2879021 MS.gene27356:CDS 55.0%
ATATGGGGGCTGTACCATGG+GGG - chr1.2:2879729-2879748 None:intergenic 55.0%
CAGAAAGCCGTGTGAGGAAG+TGG + chr1.2:2881965-2881984 MS.gene27356:CDS 55.0%
CGATATGGGGGCTGTACCAT+GGG - chr1.2:2879731-2879750 None:intergenic 55.0%
GAAAGCCGTGTGAGGAAGTG+GGG + chr1.2:2881967-2881986 MS.gene27356:CDS 55.0%
GATATGGGGGCTGTACCATG+GGG - chr1.2:2879730-2879749 None:intergenic 55.0%
GATGAGATCGCAGAAGCTGC+TGG + chr1.2:2879032-2879051 MS.gene27356:CDS 55.0%
TTCCTCCCCACTTCCTCACA+CGG - chr1.2:2881975-2881994 None:intergenic 55.0%
!! GGCAAACCCTTTGGTGCCAT+TGG + chr1.2:2880662-2880681 MS.gene27356:CDS 55.0%
!! GGCAAGGTGTTGCGATATGG+GGG - chr1.2:2879743-2879762 None:intergenic 55.0%
!! GGGCAAGGTGTTGCGATATG+GGG - chr1.2:2879744-2879763 None:intergenic 55.0%
!! TGTGGGCAAGCATTTGGTGC+TGG + chr1.2:2878888-2878907 MS.gene27356:CDS 55.0%
CGCAACACCTTGCCCTTGCT+AGG + chr1.2:2879749-2879768 MS.gene27356:intron 60.0%
GCGATATGGGGGCTGTACCA+TGG - chr1.2:2879732-2879751 None:intergenic 60.0%
AGCCGTGTGAGGAAGTGGGG+AGG + chr1.2:2881970-2881989 MS.gene27356:CDS 65.0%
GGGCTGTACCATGGGGGATC+TGG - chr1.2:2879723-2879742 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 2878335 2882014 2878335 ID=MS.gene27356
chr1.2 mRNA 2878335 2882014 2878335 ID=MS.gene27356.t1;Parent=MS.gene27356
chr1.2 exon 2878335 2878607 2878335 ID=MS.gene27356.t1.exon1;Parent=MS.gene27356.t1
chr1.2 CDS 2878335 2878607 2878335 ID=cds.MS.gene27356.t1;Parent=MS.gene27356.t1
chr1.2 exon 2878858 2879053 2878858 ID=MS.gene27356.t1.exon2;Parent=MS.gene27356.t1
chr1.2 CDS 2878858 2879053 2878858 ID=cds.MS.gene27356.t1;Parent=MS.gene27356.t1
chr1.2 exon 2880175 2880520 2880175 ID=MS.gene27356.t1.exon3;Parent=MS.gene27356.t1
chr1.2 CDS 2880175 2880520 2880175 ID=cds.MS.gene27356.t1;Parent=MS.gene27356.t1
chr1.2 exon 2880614 2880700 2880614 ID=MS.gene27356.t1.exon4;Parent=MS.gene27356.t1
chr1.2 CDS 2880614 2880700 2880614 ID=cds.MS.gene27356.t1;Parent=MS.gene27356.t1
chr1.2 exon 2880947 2881003 2880947 ID=MS.gene27356.t1.exon5;Parent=MS.gene27356.t1
chr1.2 CDS 2880947 2881003 2880947 ID=cds.MS.gene27356.t1;Parent=MS.gene27356.t1
chr1.2 exon 2881133 2881371 2881133 ID=MS.gene27356.t1.exon6;Parent=MS.gene27356.t1
chr1.2 CDS 2881133 2881371 2881133 ID=cds.MS.gene27356.t1;Parent=MS.gene27356.t1
chr1.2 exon 2881473 2881591 2881473 ID=MS.gene27356.t1.exon7;Parent=MS.gene27356.t1
chr1.2 CDS 2881473 2881591 2881473 ID=cds.MS.gene27356.t1;Parent=MS.gene27356.t1
chr1.2 exon 2881786 2881872 2881786 ID=MS.gene27356.t1.exon8;Parent=MS.gene27356.t1
chr1.2 CDS 2881786 2881872 2881786 ID=cds.MS.gene27356.t1;Parent=MS.gene27356.t1
chr1.2 exon 2881955 2882014 2881955 ID=MS.gene27356.t1.exon9;Parent=MS.gene27356.t1
chr1.2 CDS 2881955 2882014 2881955 ID=cds.MS.gene27356.t1;Parent=MS.gene27356.t1
Gene Sequence

>MS.gene27356

ATGGCTATCGACAGTCCTCTACTAGATAACAATGTCAACTCTCCTCCTAGTGTTGTTCATCATGAGGAAGAGAAGCCACTGAGAATTGTTCACAAAGTTGGAGTGGAGTCGAAGAAGCTATGGAAAATAGCCGGTCCAACAATCTTAACTTCTTTATCTCAGTACTCGCTTGGTGCATTCACTTCTACGTTTGTTGGCCATGTTAACGAGCTCGATCTTGCAGCTTTTTCTGTTGAAAATTCTGTTATTGCTGGTTTCGCCTTTGGTTTCTTGGTATGTAAACTTTTCTCATCTCCGCTTTATTATTACATTACACTTATTTTTGTTCTTTAAGGAGACTAACGACAAAACTCGCACACATTGAAAAATGTTGAGTTGTGTGGAATTGTGTGTTAGAACGCACGGGTGTACTGTTTAGTTACACTTATCTTTAACTAGTACATTTGTTTTTACCAAAAAAAAAAAAAAATGTATCCTCAAGTTTATGATTTATAAAATTGTTTTTGTTTTTGGTTGGCATCAGTTGGGTATGGGAAGTGCATTGGAGACACTATGTGGGCAAGCATTTGGTGCTGGTCATATAAGAATGATGGGAATTTACATGCAAAGATCATGGATTATCTTGGTGGCTACAGGCATTTTGTTGTTACCTTTTTATATTTGGGCAACTCCCCTCTTGAAGCTCGTTGGACAGACTGATGAGATCGCAGAAGCTGCTGGTTAGTTACTTATATAAGTTATATTCCTATCATTTAAATTTGGACTAAAACTGAAGACAGAGAATCCATTTCCGTAAATATTTCTCTTGTAAATACACTACTTTCTACTTGTTACTATTTAGTTTGAAGGGCTTAAATCGTCGGTTGTCGAAGTTTTTTACAATCATTATAGTTAGTAAGTTCATTAAGCAGCCGTATGTCTTCATTGTGACTTTTGTATATGAAAAACCCAATGTCGATCGTTCAATGTAAATTAACGGTCGAGATTATGACTATATGGAATCATGTTCCATCTTTTCACTAGTTAAAGGGATAGTTAATTTGAAGGGCTTGAATTGATCGTTGTCGAAATTTTCTACCATCATTATAGTTAGTAAGTTAATTTAAGTAGTTGGACGTCTTGCATTATGAATTAAGTATATCGAAAACTCAAAATGTCGATCGTCTGGTCTAAATTAACGATCGAGATTATGACTACAAGAGTACACAAGGAATCAAGTTCCATTTTTCGCTAATTAAAAGGGTAGCTAGGATTAGGGCCCGCTAATGTTCTCAAAGAGGGGACCAAAGGGTAAATATACTACTTTTGTGGGTCTTGTTGAACACTTTTATTAAAGCTTACATGTAAAAAACTTCGGTTCATAAAAATTTGCAGCGTAACTGGTACCAGATCCCCCATGGTACAGCCCCCATATCGCAACACCTTGCCCTTGCTAGGAAATTTTAAATGCGGATTAAAGTATAAGTACAAGATATTATCATATTAATAATGTAGCACTATAAATGCACTTGTTTGTACGAGTAAAACTATGATAATGACTTGGGGAACTTGTTATCTAGATGTGATATAACTTGTTACGACTTTATCTAACATACACAAGCAAAATATCTAACATTATATATGTATCTTTTTTACTATTCAATCTATAAATCACATTGTATCTCATTTAATTGTGATATTTATTGATTCAATAGTGGAAATTAGATTGTGCATGATACAAAACTTGTTTTACTTGTGCACCATAAAATTGACCAGGTCATTACACGATAATGGCTTGTGTGTTTGTTTGGTTGTTAGTTTCTTTTTTATATATTTATTTTATCAATTTTCCTTTTGGTAGGCACATTTGCTTTGTGGATGATACCACAAATATTTGCATATGCTTTGAACTTTCCAATTCAAAAGTTCTTACAAGCACAAAGGAAAGTCCTGGTGATGTTATGGATATCATTAGGTGTTTTAGTGTTACATGTACCTCTTAGTTGGCTTCTAATATTGAAACTTAATTGGGGCTTAGTTGGAGCTGCAATTATTCTCAACACATCATGGTGGTTGATTGTCATTGGTCAACTTTTGTACATTTTCATCACCAAATCAGATGGTGCTTGGAGTGGATTCTCATGGCTAGCATTTGCAGATTTGTTTAATTTTTTGAAACTTTCTTTAGCTTCAGCTGTTATGTTATGGTATATTTTCTCAAACTTTATATTTTCAATTCAACTTAATTAATATTTGATGGAAACTTTATATTGAATTGAATTTTGAAAATGTGATGGCAGCTTGGAGTTTTGGTACTTGATGATATTGGTGGTGATAACAGGGCATTTGGCAAACCCTTTGGTGCCATTGGATGCTGTATCTATTTGGTAAACCCTTTCTCCAAATTCTCATTGTGCTATAGAAGTTATGGTTTTCCAAATAAAAGTGATAGACATTTTCTATTTTGTTCCTAGCACTTTTGCATTGTTGTGTGATTGCAATTCAAAACTGAGCATTAATTAGGCTTGGGTTAGTCATTGTAAGCATATCATCCAATTGCAGTATACAAGGAGAACATGACAACAAAAGTTAAGATCAAGAATTGATATTGATGTGTGTTTCCATGATTGCAGTATGAACATAAATGGTTGGGATATGATGATCGCGCTAGGGTTTAACGTTGCAATAAGGTCATTATCGCTTTCTCTATTTCTTTTCTTGACAAACTTACCTTCTTTAAAGTGAACAACTTATTTTCTTTTCTTGAGTGAATAGTTAGTCGCAGTTTTCTATACTGATTATGTTTAATTTATGAACAGTGTGAGAGTATCAAATGAACTTGGAGCAGGTGATTTTGAAGCTGCAAAGTTCTCAGTGATAGTGGTTTCTCTCACATCAATATCCATTGGTGTTGTTGCCATGATCATAGTACTAACAACTAGAGATTACTTTCCTCAATTGTTCACTTCAAGCTATGAGGTTGCCCAGGAAACAACTAAACTTGCTGCTTTGCTTAGTATCACTGTACTTCTAAACAGCCTACAACCAGTCTTGTCTGGTTAGTCACAAAAAGATTGAAACATTGTTACTATAAATGTTTTTATGCATTGAAACATTGTTACTATAAATTAATTTATGCATTTGTTGTTACTATTTTAGGTGTTGCTATTGGAGCTGGTTGGCAATCTCTTGTAGCTTACATAAATCTTGGATCCTACTATGTTGTTGGATTACCAGCTGGGATATTGTTGGGCTTCACATTTGGTTTTGGAGCAGAGGTGATTTACTACCCCTTATTTCTCTTGAATTTAATTCTACAACACTTTAAATATATAGTATAATTTTTAGAAGGATTCATTTAGTAGTTTAATATTTATTGTTTGTTAATGAGGACTATTTTGTAATTTTTTATTGTATTATCAACATAAATTTATTTGTTCTTACTTTTTTTATACCTATGATTTGTTATCAGGGTATTTGGTCAGGTCTGATTGGAGGCATAGCTGTGCAGACCATAATCCTTATAATTGTTACTTCATTAACCAACTGGAAAAAAGAGGTTAGTGCAATTCATTTTTGTATTAGCAAATAAATAACCTATTGTTTGTTGAAAATTAATTTCATATCTTTGGATTTCACAGGCTGATGAAGCAGAAAGCCGTGTGAGGAAGTGGGGAGGAACAATTTCAATTGAGAATTAA
Protein sequence

>MS.gene27356.t1

MAIDSPLLDNNVNSPPSVVHHEEEKPLRIVHKVGVESKKLWKIAGPTILTSLSQYSLGAFTSTFVGHVNELDLAAFSVENSVIAGFAFGFLLGMGSALETLCGQAFGAGHIRMMGIYMQRSWIILVATGILLLPFYIWATPLLKLVGQTDEIAEAAGTFALWMIPQIFAYALNFPIQKFLQAQRKVLVMLWISLGVLVLHVPLSWLLILKLNWGLVGAAIILNTSWWLIVIGQLLYIFITKSDGAWSGFSWLAFADLFNFLKLSLASAVMLCLEFWYLMILVVITGHLANPLVPLDAVSICMNINGWDMMIALGFNVAISVRVSNELGAGDFEAAKFSVIVVSLTSISIGVVAMIIVLTTRDYFPQLFTSSYEVAQETTKLAALLSITVLLNSLQPVLSGVAIGAGWQSLVAYINLGSYYVVGLPAGILLGFTFGFGAEGIWSGLIGGIAVQTIILIIVTSLTNWKKEADEAESRVRKWGGTISIEN