Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27400.t1 | XP_003620064.1 | 98.8 | 592 | 3 | 1 | 1 | 592 | 1 | 588 | 0.00E+00 | 1145.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27400.t1 | Q9ZPS7 | 86.6 | 591 | 73 | 3 | 6 | 592 | 4 | 592 | 1.9e-290 | 999.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27400.t1 | G7KJN8 | 98.8 | 592 | 3 | 1 | 1 | 592 | 1 | 588 | 0.0e+00 | 1145.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051485 | MS.gene27400 | 0.812321 | 4.50E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27400.t1 | MTR_6g074910 | 98.818 | 592 | 3 | 1 | 1 | 592 | 1 | 588 | 0.0 | 1204 |
| MS.gene27400.t1 | MTR_5g077170 | 77.739 | 575 | 126 | 1 | 20 | 592 | 17 | 591 | 0.0 | 946 |
| MS.gene27400.t1 | MTR_4g053270 | 47.909 | 574 | 293 | 3 | 21 | 592 | 20 | 589 | 0.0 | 565 |
| MS.gene27400.t1 | MTR_8g088730 | 39.410 | 576 | 333 | 6 | 25 | 592 | 22 | 589 | 6.69e-145 | 432 |
| MS.gene27400.t1 | MTR_2g006300 | 40.522 | 575 | 326 | 7 | 25 | 592 | 18 | 583 | 3.23e-144 | 430 |
| MS.gene27400.t1 | MTR_8g016270 | 33.386 | 632 | 358 | 9 | 12 | 592 | 29 | 648 | 4.63e-125 | 382 |
| MS.gene27400.t1 | MTR_1g014080 | 32.248 | 614 | 361 | 9 | 28 | 592 | 30 | 637 | 1.55e-110 | 345 |
| MS.gene27400.t1 | MTR_3g105950 | 32.237 | 608 | 357 | 8 | 34 | 592 | 35 | 636 | 1.39e-109 | 342 |
| MS.gene27400.t1 | MTR_5g098730 | 26.941 | 631 | 382 | 16 | 32 | 592 | 44 | 665 | 3.23e-74 | 250 |
| MS.gene27400.t1 | MTR_3g082140 | 26.689 | 607 | 369 | 13 | 55 | 592 | 60 | 659 | 5.06e-74 | 249 |
| MS.gene27400.t1 | MTR_0204s0010 | 30.110 | 455 | 289 | 9 | 156 | 592 | 5 | 448 | 9.39e-73 | 240 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27400.t1 | AT2G01970 | 88.307 | 573 | 66 | 1 | 21 | 592 | 20 | 592 | 0.0 | 1038 |
| MS.gene27400.t1 | AT1G14670 | 87.916 | 571 | 68 | 1 | 23 | 592 | 22 | 592 | 0.0 | 1006 |
| MS.gene27400.t1 | AT5G37310 | 83.624 | 574 | 93 | 1 | 20 | 592 | 20 | 593 | 0.0 | 978 |
| MS.gene27400.t1 | AT5G37310 | 83.624 | 574 | 93 | 1 | 20 | 592 | 20 | 593 | 0.0 | 978 |
| MS.gene27400.t1 | AT1G08350 | 50.613 | 571 | 276 | 4 | 25 | 592 | 22 | 589 | 0.0 | 615 |
| MS.gene27400.t1 | AT1G08350 | 50.613 | 571 | 276 | 4 | 25 | 592 | 22 | 589 | 0.0 | 615 |
| MS.gene27400.t1 | AT1G08350 | 48.924 | 511 | 255 | 4 | 85 | 592 | 1 | 508 | 0.0 | 530 |
| MS.gene27400.t1 | AT1G08350 | 48.362 | 519 | 253 | 4 | 25 | 540 | 22 | 528 | 0.0 | 528 |
| MS.gene27400.t1 | AT1G10950 | 39.373 | 574 | 334 | 6 | 25 | 592 | 24 | 589 | 7.28e-142 | 424 |
| MS.gene27400.t1 | AT3G13772 | 31.922 | 614 | 359 | 8 | 30 | 592 | 36 | 641 | 6.75e-119 | 366 |
| MS.gene27400.t1 | AT1G55130 | 32.543 | 633 | 364 | 9 | 15 | 592 | 13 | 637 | 9.37e-119 | 366 |
| MS.gene27400.t1 | AT2G24170 | 32.248 | 645 | 376 | 8 | 1 | 592 | 1 | 637 | 2.40e-116 | 360 |
| MS.gene27400.t1 | AT5G10840 | 31.707 | 615 | 364 | 8 | 28 | 592 | 40 | 648 | 4.60e-110 | 344 |
| MS.gene27400.t1 | AT5G25100 | 31.280 | 617 | 366 | 7 | 28 | 592 | 34 | 644 | 1.31e-109 | 342 |
| MS.gene27400.t1 | AT5G25100 | 31.639 | 610 | 359 | 7 | 35 | 592 | 48 | 651 | 4.48e-109 | 341 |
| MS.gene27400.t1 | AT5G35160 | 26.972 | 634 | 383 | 16 | 31 | 592 | 33 | 658 | 5.77e-71 | 241 |
| MS.gene27400.t1 | AT5G35160 | 26.972 | 634 | 383 | 16 | 31 | 592 | 33 | 658 | 5.77e-71 | 241 |
| MS.gene27400.t1 | AT5G35160 | 26.972 | 634 | 383 | 16 | 31 | 592 | 33 | 658 | 5.77e-71 | 241 |
| MS.gene27400.t1 | AT5G35160 | 26.968 | 597 | 358 | 14 | 67 | 592 | 41 | 630 | 3.04e-66 | 228 |
| MS.gene27400.t1 | AT4G12650 | 29.237 | 472 | 308 | 10 | 136 | 592 | 192 | 652 | 1.14e-62 | 218 |
| MS.gene27400.t1 | AT2G24170 | 27.938 | 451 | 265 | 7 | 1 | 398 | 1 | 444 | 3.58e-58 | 201 |
Find 147 sgRNAs with CRISPR-Local
Find 306 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTATGATGATTTGCCTATT+TGG | 0.197680 | 6.4:+50321700 | MS.gene27400:CDS |
| ATTATATATTTGCCAGTGTC+TGG | 0.215685 | 6.4:+50323066 | MS.gene27400:CDS |
| TTATGATGATTTGCCTATTT+GGG | 0.236802 | 6.4:+50321701 | MS.gene27400:CDS |
| TTGCCTATTTGGGGTTTCAT+AGG | 0.302131 | 6.4:+50321711 | MS.gene27400:CDS |
| CTTGTATTGGGAGGGATTGC+TGG | 0.316117 | 6.4:+50322897 | MS.gene27400:CDS |
| TACGTCTCCTCTACTTGTAT+TGG | 0.316329 | 6.4:+50322884 | MS.gene27400:CDS |
| GTTATATATGCACTCACTTC+AGG | 0.319264 | 6.4:+50322557 | MS.gene27400:CDS |
| TCCTCCTTTGCCATGGTATC+GGG | 0.329441 | 6.4:+50322980 | MS.gene27400:CDS |
| TTCTTGATGCTTGGAACTGT+TGG | 0.329989 | 6.4:+50324337 | MS.gene27400:CDS |
| TTCCTATGCGGTGGTTCAAC+TGG | 0.342782 | 6.4:+50324202 | MS.gene27400:CDS |
| TACGGTGTTCATATTCATAT+TGG | 0.349084 | 6.4:+50322478 | MS.gene27400:CDS |
| ACGTCTCCTCTACTTGTATT+GGG | 0.350290 | 6.4:+50322885 | MS.gene27400:CDS |
| TGTTATAAAATATATCAAAA+TGG | 0.350719 | 6.4:-50321794 | None:intergenic |
| AACCAACCTGGTATACAAAA+TGG | 0.355606 | 6.4:-50320543 | None:intergenic |
| ACTTGACAAAATCATTCTTA+AGG | 0.370613 | 6.4:-50322076 | None:intergenic |
| GTAACATCCACAAGGGCATT+TGG | 0.374033 | 6.4:-50321852 | None:intergenic |
| GGAGATGAAGAGAGAAGAAA+TGG | 0.385785 | 6.4:-50320027 | None:intergenic |
| AAATGCCTACTCGCACTTGA+TGG | 0.387744 | 6.4:-50324401 | None:intergenic |
| AAGAAGGCGAGGATGAATAA+TGG | 0.388893 | 6.4:-50320002 | None:intergenic |
| TTGTCGTTATAGTTCTCATA+TGG | 0.395244 | 6.4:+50322853 | MS.gene27400:CDS |
| TCTGTATTTGCAGCAGCTCT+TGG | 0.397933 | 6.4:+50322312 | MS.gene27400:CDS |
| ATATTCATATTGGCACTTGT+TGG | 0.402301 | 6.4:+50322488 | MS.gene27400:CDS |
| TTCCTCCTTTGCCATGGTAT+CGG | 0.402307 | 6.4:+50322979 | MS.gene27400:CDS |
| TGAGGAGACTGCAGAGGATC+AGG | 0.408156 | 6.4:+50322233 | MS.gene27400:CDS |
| TTCCCTATGAAACCCCAAAT+AGG | 0.409018 | 6.4:-50321714 | None:intergenic |
| TTTGCAGCAGCTCTTGGATC+TGG | 0.409525 | 6.4:+50322318 | MS.gene27400:CDS |
| ACCAGTGGATTTCCAAATGA+TGG | 0.412001 | 6.4:-50321983 | None:intergenic |
| GTCAACTGGAAGGAACAAAC+TGG | 0.416389 | 6.4:+50322612 | MS.gene27400:CDS |
| TTTGGTTTCTTCTTGATGCT+TGG | 0.416603 | 6.4:+50324328 | MS.gene27400:CDS |
| GTCCTCGCTACCCCATCATT+TGG | 0.419122 | 6.4:+50321971 | MS.gene27400:CDS |
| TACATGGCCTGCATATGCTT+TGG | 0.422165 | 6.4:+50324310 | MS.gene27400:CDS |
| CACTGGGTGAAGTGTTGAAT+GGG | 0.423169 | 6.4:+50321538 | MS.gene27400:CDS |
| TTATATATTTGCCAGTGTCT+GGG | 0.430768 | 6.4:+50323067 | MS.gene27400:CDS |
| TTTCATAGGGAAGGTTGATA+AGG | 0.446067 | 6.4:+50321725 | MS.gene27400:CDS |
| GGATATTTAGTTGTGCGGAC+TGG | 0.447634 | 6.4:-50322948 | None:intergenic |
| GAGAGAAGAAATGGAGAAGA+AGG | 0.451582 | 6.4:-50320018 | None:intergenic |
| TGCAAATACAGATTTGAACT+TGG | 0.452592 | 6.4:-50322301 | None:intergenic |
| GTCACAGTCCTCCTCTTGAC+TGG | 0.456631 | 6.4:+50322026 | MS.gene27400:CDS |
| GAAATAGAGGCCCACAGAAA+AGG | 0.459642 | 6.4:-50322761 | None:intergenic |
| AAGCAAAATGTAAGAAATAG+AGG | 0.470399 | 6.4:-50322774 | None:intergenic |
| CCCATCATTTGGAAATCCAC+TGG | 0.476786 | 6.4:+50321982 | MS.gene27400:CDS |
| GAAAGAGAAGAAAGAAGCAC+TGG | 0.478603 | 6.4:+50321521 | MS.gene27400:CDS |
| ATAGTAGTAATACAGACAAT+AGG | 0.479087 | 6.4:-50324239 | None:intergenic |
| TATGCCCGCTCCGATATGTC+TGG | 0.480652 | 6.4:+50324259 | MS.gene27400:CDS |
| TCTGATAAGCGTTACAAAGA+AGG | 0.490013 | 6.4:+50320068 | MS.gene27400:CDS |
| TACCTCATTCTATTGTCAAC+TGG | 0.491307 | 6.4:+50322598 | MS.gene27400:CDS |
| AATAGAGCCCCTCGGTTGTA+CGG | 0.492094 | 6.4:-50322521 | None:intergenic |
| TTGCATGAAACCAGACATAT+CGG | 0.492755 | 6.4:-50324269 | None:intergenic |
| AACTCACAGGGTAAACAGTT+GGG | 0.494448 | 6.4:-50322343 | None:intergenic |
| TAACGACAATTGTGCCGAAT+GGG | 0.498949 | 6.4:-50322839 | None:intergenic |
| TTGTATTGGGAGGGATTGCT+GGG | 0.499754 | 6.4:+50322898 | MS.gene27400:CDS |
| GCAAATACAGATTTGAACTT+GGG | 0.506096 | 6.4:-50322300 | None:intergenic |
| AAGTACCTTGGATTATGAAA+AGG | 0.506207 | 6.4:-50320125 | None:intergenic |
| AATTCTGTGGCCCCAGACAC+TGG | 0.506214 | 6.4:-50323078 | None:intergenic |
| TATTTGGGGTTTCATAGGGA+AGG | 0.508176 | 6.4:+50321716 | MS.gene27400:CDS |
| AGGATCAGGAAGAGACTGGC+TGG | 0.510925 | 6.4:+50322247 | MS.gene27400:CDS |
| TGCCTATTTGGGGTTTCATA+GGG | 0.512616 | 6.4:+50321712 | MS.gene27400:CDS |
| GCTACTGCCGCTCTCCCATT+CGG | 0.515377 | 6.4:+50322825 | MS.gene27400:CDS |
| CCTCTCTTCGCTAACAAAGT+TGG | 0.516706 | 6.4:+50320101 | MS.gene27400:CDS |
| CCAGTGGATTTCCAAATGAT+GGG | 0.517728 | 6.4:-50321982 | None:intergenic |
| CAAAATGGATGTGCTTGTAA+AGG | 0.520184 | 6.4:-50321779 | None:intergenic |
| ATAGGGAAGGTTGATAAGGA+GGG | 0.521709 | 6.4:+50321729 | MS.gene27400:CDS |
| CTCCTCTACTTGTATTGGGA+GGG | 0.524300 | 6.4:+50322889 | MS.gene27400:CDS |
| GGCAAAGGAGGAATCTCCCT+TGG | 0.527338 | 6.4:-50322969 | None:intergenic |
| GACACTTACAAAAGAAGAAG+TGG | 0.527829 | 6.4:+50321632 | MS.gene27400:CDS |
| CATGATCTTCAGCAGCAAGC+TGG | 0.529624 | 6.4:-50323184 | None:intergenic |
| CCCTTGGATATTTAGTTGTG+CGG | 0.531342 | 6.4:-50322953 | None:intergenic |
| AAACTCACAGGGTAAACAGT+TGG | 0.533116 | 6.4:-50322344 | None:intergenic |
| AGTGTTGAATGGGGATAGGT+TGG | 0.533187 | 6.4:+50321548 | MS.gene27400:CDS |
| TATGATGATTTGCCTATTTG+GGG | 0.533815 | 6.4:+50321702 | MS.gene27400:CDS |
| GCACTCACTTCAGGAATCGC+TGG | 0.536391 | 6.4:+50322566 | MS.gene27400:CDS |
| TTTCTATACTGTGAAATGGA+AGG | 0.537931 | 6.4:+50321902 | MS.gene27400:CDS |
| ATTCCTCAGATGGCAATGGC+AGG | 0.540197 | 6.4:+50323008 | MS.gene27400:CDS |
| CATAGGGAAGGTTGATAAGG+AGG | 0.540980 | 6.4:+50321728 | MS.gene27400:CDS |
| TAGTTGTGCGGACTGGAGCT+TGG | 0.548078 | 6.4:-50322941 | None:intergenic |
| GCACTGGGTGAAGTGTTGAA+TGG | 0.549117 | 6.4:+50321537 | MS.gene27400:CDS |
| AAATGATGGGGTAGCGAGGA+CGG | 0.550197 | 6.4:-50321969 | None:intergenic |
| GCAGAGGATCAGGAAGAGAC+TGG | 0.553267 | 6.4:+50322243 | MS.gene27400:CDS |
| AGGTAGTATCTATAATCACT+AGG | 0.554450 | 6.4:-50321759 | None:intergenic |
| TATATATTTGCCAGTGTCTG+GGG | 0.557505 | 6.4:+50323068 | MS.gene27400:CDS |
| AAGAAATGGAGAAGAAGGCG+AGG | 0.559753 | 6.4:-50320013 | None:intergenic |
| AGGCCCACAGAAAAGGCATC+CGG | 0.561889 | 6.4:-50322754 | None:intergenic |
| GAAGAAACCAAAGCATATGC+AGG | 0.562481 | 6.4:-50324317 | None:intergenic |
| CCCCGATACCATGGCAAAGG+AGG | 0.562801 | 6.4:-50322981 | None:intergenic |
| GAAACCAGACATATCGGAGC+GGG | 0.562807 | 6.4:-50324263 | None:intergenic |
| GGTAGTATCTATAATCACTA+GGG | 0.562961 | 6.4:-50321758 | None:intergenic |
| CTGAAGATCATGAATGGTGG+TGG | 0.564533 | 6.4:+50323195 | MS.gene27400:CDS |
| TACAGATCATTCCTATGCGG+TGG | 0.566423 | 6.4:+50324193 | MS.gene27400:intron |
| ATCCCTCCCAATACAAGTAG+AGG | 0.569721 | 6.4:-50322891 | None:intergenic |
| CACTCCCTTCGAGAAGCGGA+TGG | 0.569874 | 6.4:+50321932 | MS.gene27400:CDS |
| TTGTCCATCCGCTTCTCGAA+GGG | 0.570473 | 6.4:-50321936 | None:intergenic |
| GTGAAGTGTTGAATGGGGAT+AGG | 0.575284 | 6.4:+50321544 | MS.gene27400:CDS |
| TGTTACTGCTTTCATTACTG+TGG | 0.578518 | 6.4:+50323148 | MS.gene27400:CDS |
| AACGGATCCAAATGCCCTTG+TGG | 0.580190 | 6.4:+50321845 | MS.gene27400:CDS |
| CTTGTCCATCCGCTTCTCGA+AGG | 0.580261 | 6.4:-50321937 | None:intergenic |
| TCGGGGAGCAATTCCTCAGA+TGG | 0.580703 | 6.4:+50322998 | MS.gene27400:CDS |
| TTGCTGCTGAAGATCATGAA+TGG | 0.582525 | 6.4:+50323189 | MS.gene27400:CDS |
| TTCCAGTTGACAATAGAATG+AGG | 0.583452 | 6.4:-50322600 | None:intergenic |
| AATCCTGCCATTGCCATCTG+AGG | 0.585284 | 6.4:-50323011 | None:intergenic |
| CCTACTCGCACTTGATGGAT+CGG | 0.585534 | 6.4:-50324396 | None:intergenic |
| CCAACTTTGTTAGCGAAGAG+AGG | 0.587195 | 6.4:-50320101 | None:intergenic |
| CTAACACTCCCTTCGAGAAG+CGG | 0.587277 | 6.4:+50321928 | MS.gene27400:CDS |
| GGGAGATTCCTCCTTTGCCA+TGG | 0.590122 | 6.4:+50322973 | MS.gene27400:CDS |
| TCCGCACAACTAAATATCCA+AGG | 0.591816 | 6.4:+50322952 | MS.gene27400:CDS |
| ATCAGATGAATCAGATCTGA+CGG | 0.591852 | 6.4:-50320051 | None:intergenic |
| TCTCCTCTACTTGTATTGGG+AGG | 0.599263 | 6.4:+50322888 | MS.gene27400:CDS |
| GTTCGGAATCTACTATTGAC+CGG | 0.603844 | 6.4:+50322735 | MS.gene27400:CDS |
| ATAACGACAATTGTGCCGAA+TGG | 0.606594 | 6.4:-50322840 | None:intergenic |
| TTGCAAGAAAACCAGTCAAG+AGG | 0.612188 | 6.4:-50322037 | None:intergenic |
| CCTCCTTTGCCATGGTATCG+GGG | 0.612898 | 6.4:+50322981 | MS.gene27400:CDS |
| GCTCCCCGATACCATGGCAA+AGG | 0.619903 | 6.4:-50322984 | None:intergenic |
| TTCCAAATGATGGGGTAGCG+AGG | 0.620376 | 6.4:-50321973 | None:intergenic |
| TCGATGTAAATAGCACTGAA+AGG | 0.620759 | 6.4:-50323038 | None:intergenic |
| AAACAGGTATGCACATGATG+AGG | 0.621763 | 6.4:+50322215 | MS.gene27400:intron |
| CCGATCCATCAAGTGCGAGT+AGG | 0.623339 | 6.4:+50324396 | MS.gene27400:CDS |
| GGAATTGCTCCCCGATACCA+TGG | 0.625145 | 6.4:-50322990 | None:intergenic |
| AATTGATAATCGAAAACCAG+TGG | 0.626537 | 6.4:-50321998 | None:intergenic |
| TCATTCTATTGTCAACTGGA+AGG | 0.630520 | 6.4:+50322602 | MS.gene27400:CDS |
| ACTTGATGGATCGGTATATG+TGG | 0.631167 | 6.4:-50324387 | None:intergenic |
| CAAGAAAACCAGTCAAGAGG+AGG | 0.631428 | 6.4:-50322034 | None:intergenic |
| AGCAATTCCTCAGATGGCAA+TGG | 0.634595 | 6.4:+50323004 | MS.gene27400:CDS |
| AAGATCATGAATGGTGGTGG+AGG | 0.635786 | 6.4:+50323198 | MS.gene27400:CDS |
| TATTGAGATCAATGTGCGAA+CGG | 0.636703 | 6.4:+50321827 | MS.gene27400:CDS |
| ATCCAGTTGAACCACCGCAT+AGG | 0.639943 | 6.4:-50324204 | None:intergenic |
| GTGCAGTGAATAGAGCCCCT+CGG | 0.643541 | 6.4:-50322529 | None:intergenic |
| AATTGTGCCGAATGGGAGAG+CGG | 0.644658 | 6.4:-50322832 | None:intergenic |
| TAGGGAAGGTTGATAAGGAG+GGG | 0.648260 | 6.4:+50321730 | MS.gene27400:CDS |
| TGCCCTTGTGGATGTTACAG+AGG | 0.650587 | 6.4:+50321857 | MS.gene27400:CDS |
| AAAAGACAAGGACTCTGTGT+CGG | 0.658291 | 6.4:+50321599 | MS.gene27400:CDS |
| CTGCTGAAGATCATGAATGG+TGG | 0.659388 | 6.4:+50323192 | MS.gene27400:CDS |
| CCGCACAACTAAATATCCAA+GGG | 0.660490 | 6.4:+50322953 | MS.gene27400:CDS |
| CAGTGGATTTCCAAATGATG+GGG | 0.661541 | 6.4:-50321981 | None:intergenic |
| TGATGGATCGGTATATGTGG+CGG | 0.664035 | 6.4:-50324384 | None:intergenic |
| TGAAACCAGACATATCGGAG+CGG | 0.664795 | 6.4:-50324264 | None:intergenic |
| TGTCCTCTGTAACATCCACA+AGG | 0.669975 | 6.4:-50321860 | None:intergenic |
| AAAGAGAAGAAAGAAGCACT+GGG | 0.674148 | 6.4:+50321522 | MS.gene27400:CDS |
| GGACAAAGAACAATGCTGCA+CGG | 0.675721 | 6.4:-50324363 | None:intergenic |
| TCAACTGGAAGGAACAAACT+GGG | 0.689021 | 6.4:+50322613 | MS.gene27400:CDS |
| CCTTACAGATCATTCCTATG+CGG | 0.691017 | 6.4:+50324190 | MS.gene27400:intron |
| TGTAGATTGTGTAAATTCTG+TGG | 0.694009 | 6.4:-50323091 | None:intergenic |
| ACATGATGAGGAGACTGCAG+AGG | 0.702295 | 6.4:+50322227 | MS.gene27400:CDS |
| GATTGCTGGGAAGAACAGCA+AGG | 0.708765 | 6.4:+50322911 | MS.gene27400:CDS |
| AGATGAATCAGATCTGACGG+TGG | 0.709112 | 6.4:-50320048 | None:intergenic |
| ACTGGCTGGAAATACATCCA+CGG | 0.709634 | 6.4:+50322261 | MS.gene27400:CDS |
| GTCCTCTGTAACATCCACAA+GGG | 0.709898 | 6.4:-50321859 | None:intergenic |
| GTAGTAGTCCTTACGAACAG+CGG | 0.726687 | 6.4:-50321667 | None:intergenic |
| ACTGGGTGAAGTGTTGAATG+GGG | 0.747764 | 6.4:+50321539 | MS.gene27400:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGTTATAAAATATATCAAAA+TGG | - | chr6.4:50321797-50321816 | None:intergenic | 10.0% |
| !!! | AAGTTTTAATTTTTAAATCA+TGG | + | chr6.4:50320607-50320626 | MS.gene27400:intron | 10.0% |
| !!! | ATGTTTTAATTTTTAAATCA+TGG | + | chr6.4:50320737-50320756 | MS.gene27400:intron | 10.0% |
| !!! | ATTTTAACAAAATAATAAAC+TGG | - | chr6.4:50323549-50323568 | None:intergenic | 10.0% |
| !!! | TTTTGATATATTTTATAACA+AGG | + | chr6.4:50321797-50321816 | MS.gene27400:CDS | 10.0% |
| !! | AAAAAAAACACAAATAGAAA+AGG | - | chr6.4:50322108-50322127 | None:intergenic | 15.0% |
| !! | ATTAATGATAAGAAAATCAA+AGG | + | chr6.4:50320956-50320975 | MS.gene27400:intron | 15.0% |
| !! | TAAGTAGTAAATATGAATTA+TGG | + | chr6.4:50323386-50323405 | MS.gene27400:intron | 15.0% |
| !!! | ATGCAAATGTTTTAAAATAT+CGG | + | chr6.4:50320995-50321014 | MS.gene27400:intron | 15.0% |
| !!! | ATTTTGAGATATTTTCTTTT+TGG | + | chr6.4:50321460-50321479 | MS.gene27400:intron | 15.0% |
| !!! | TTCTATTTGTGTTTTTTTTT+TGG | + | chr6.4:50322109-50322128 | MS.gene27400:intron | 15.0% |
| !!! | TTTTAAATCATGGTTATTTA+GGG | + | chr6.4:50320747-50320766 | MS.gene27400:intron | 15.0% |
| !!! | TTTTCTTATCATTAATATCA+AGG | - | chr6.4:50320952-50320971 | None:intergenic | 15.0% |
| !!! | TTTTGAGATATTTTCTTTTT+GGG | + | chr6.4:50321461-50321480 | MS.gene27400:intron | 15.0% |
| !!! | TTTTTAAATCATGGTTATTT+AGG | + | chr6.4:50320746-50320765 | MS.gene27400:intron | 15.0% |
| !! | AAACAATAAACCATAGAAAT+TGG | - | chr6.4:50323261-50323280 | None:intergenic | 20.0% |
| !! | AAATCATGATGTTAAAACAT+GGG | + | chr6.4:50321114-50321133 | MS.gene27400:intron | 20.0% |
| !! | AATTAAAGAAAACTCATTTC+AGG | + | chr6.4:50322389-50322408 | MS.gene27400:intron | 20.0% |
| !! | CTTTAATTGAACATATTGAA+TGG | - | chr6.4:50322377-50322396 | None:intergenic | 20.0% |
| !! | TCAATTCTTAAATCATGTAA+CGG | - | chr6.4:50321060-50321079 | None:intergenic | 20.0% |
| !!! | AAATAACTTGTCAAAACATT+AGG | - | chr6.4:50323832-50323851 | None:intergenic | 20.0% |
| !!! | ACTTATAAATCTATGCATTT+TGG | + | chr6.4:50322641-50322660 | MS.gene27400:intron | 20.0% |
| !!! | ATTCTGTCACATTTTTTAAT+TGG | + | chr6.4:50320251-50320270 | MS.gene27400:intron | 20.0% |
| !!! | TGGTTAATATCATTTTATTG+CGG | + | chr6.4:50320659-50320678 | MS.gene27400:intron | 20.0% |
| !!! | TGTTTTTTACAAAGTAAACA+TGG | + | chr6.4:50323806-50323825 | MS.gene27400:intron | 20.0% |
| !!! | TTCTGTCACATTTTTTAATT+GGG | + | chr6.4:50320252-50320271 | MS.gene27400:intron | 20.0% |
| !!! | TTTGAGATATTTTCTTTTTG+GGG | + | chr6.4:50321462-50321481 | MS.gene27400:intron | 20.0% |
| !!! | TTTTAAATCATGGTTATTCA+GGG | + | chr6.4:50320617-50320636 | MS.gene27400:intron | 20.0% |
| !!! | TTTTGAAGTGAATTATTTGA+TGG | + | chr6.4:50323606-50323625 | MS.gene27400:intron | 20.0% |
| !!! | TTTTTAAATCATGGTTATTC+AGG | + | chr6.4:50320616-50320635 | MS.gene27400:intron | 20.0% |
| ! | AAATAAAGAAAGACACAAAG+CGG | - | chr6.4:50321235-50321254 | None:intergenic | 25.0% |
| ! | AAGCAAAATGTAAGAAATAG+AGG | - | chr6.4:50322777-50322796 | None:intergenic | 25.0% |
| ! | ACTTGACAAAATCATTCTTA+AGG | - | chr6.4:50322079-50322098 | None:intergenic | 25.0% |
| ! | AGCAAAAGAATAAATAACAG+AGG | - | chr6.4:50322164-50322183 | None:intergenic | 25.0% |
| ! | ATAGTAGTAATACAGACAAT+AGG | - | chr6.4:50324242-50324261 | None:intergenic | 25.0% |
| ! | ATTAGTAATCTTTACTGTGT+TGG | + | chr6.4:50323868-50323887 | MS.gene27400:intron | 25.0% |
| ! | ATTATTTGATGGAGAGAATA+TGG | + | chr6.4:50323617-50323636 | MS.gene27400:intron | 25.0% |
| ! | CAAATCATGATGTTAAAACA+TGG | + | chr6.4:50321113-50321132 | MS.gene27400:intron | 25.0% |
| ! | CGTAGCTAAATAAAAAAACA+TGG | + | chr6.4:50321281-50321300 | MS.gene27400:intron | 25.0% |
| ! | TACTAGTAATACCTAACTAA+GGG | - | chr6.4:50323290-50323309 | None:intergenic | 25.0% |
| !! | AGTTTTTCTATACTGTGAAA+TGG | + | chr6.4:50321898-50321917 | MS.gene27400:CDS | 25.0% |
| !! | ATTATGATGATTTGCCTATT+TGG | + | chr6.4:50321700-50321719 | MS.gene27400:CDS | 25.0% |
| !! | GACAAGTTATTTCAAACTTA+AGG | + | chr6.4:50323840-50323859 | MS.gene27400:intron | 25.0% |
| !! | GATGACTCAATATTTAACTT+TGG | + | chr6.4:50321411-50321430 | MS.gene27400:intron | 25.0% |
| !! | GGTTAATATCATTTTATTGC+GGG | + | chr6.4:50320660-50320679 | MS.gene27400:intron | 25.0% |
| !! | TCAATATTTAACTTTGGAAG+AGG | + | chr6.4:50321417-50321436 | MS.gene27400:intron | 25.0% |
| !! | TTATGATGATTTGCCTATTT+GGG | + | chr6.4:50321701-50321720 | MS.gene27400:CDS | 25.0% |
| !! | TTTCTTTATTTGAGAATGAG+TGG | + | chr6.4:50321244-50321263 | MS.gene27400:intron | 25.0% |
| !!! | AGGGTATTGATTTTAAATCA+TGG | + | chr6.4:50320766-50320785 | MS.gene27400:intron | 25.0% |
| !!! | ATGTTTTAAAATATCGGATC+CGG | + | chr6.4:50321001-50321020 | MS.gene27400:intron | 25.0% |
| !!! | GGGTATTGATTTTAAATCAT+GGG | + | chr6.4:50320767-50320786 | MS.gene27400:intron | 25.0% |
| !!! | GTTTTAAGTTATGGTTATGT+TGG | + | chr6.4:50320639-50320658 | MS.gene27400:intron | 25.0% |
| !!! | TTATTCAGGGTTTTAAGTTA+TGG | + | chr6.4:50320630-50320649 | MS.gene27400:intron | 25.0% |
| AAGTACCTTGGATTATGAAA+AGG | - | chr6.4:50320128-50320147 | None:intergenic | 30.0% | |
| AATAAAGAAAGACACAAAGC+GGG | - | chr6.4:50321234-50321253 | None:intergenic | 30.0% | |
| AATATGGAATTCTTGCTTAG+GGG | + | chr6.4:50323633-50323652 | MS.gene27400:intron | 30.0% | |
| ACTAGTAATACCTAACTAAG+GGG | - | chr6.4:50323289-50323308 | None:intergenic | 30.0% | |
| ACTGATAAGAAAATCAGTTG+TGG | - | chr6.4:50321385-50321404 | None:intergenic | 30.0% | |
| AGAATATGGAATTCTTGCTT+AGG | + | chr6.4:50323631-50323650 | MS.gene27400:intron | 30.0% | |
| AGGTAGTATCTATAATCACT+AGG | - | chr6.4:50321762-50321781 | None:intergenic | 30.0% | |
| ATATTCATATTGGCACTTGT+TGG | + | chr6.4:50322488-50322507 | MS.gene27400:CDS | 30.0% | |
| ATTATATATTTGCCAGTGTC+TGG | + | chr6.4:50323066-50323085 | MS.gene27400:CDS | 30.0% | |
| ATTTCTTGCACCAATTTCTA+TGG | + | chr6.4:50323248-50323267 | MS.gene27400:intron | 30.0% | |
| CATGTATCCAAAGAAAAATG+AGG | - | chr6.4:50324296-50324315 | None:intergenic | 30.0% | |
| CTTTCATAATGAATCACATG+AGG | + | chr6.4:50324089-50324108 | MS.gene27400:intron | 30.0% | |
| GAAAATCAGTTGTGGTAATT+TGG | - | chr6.4:50321377-50321396 | None:intergenic | 30.0% | |
| GAATATGGAATTCTTGCTTA+GGG | + | chr6.4:50323632-50323651 | MS.gene27400:intron | 30.0% | |
| GCAAATACAGATTTGAACTT+GGG | - | chr6.4:50322303-50322322 | None:intergenic | 30.0% | |
| GGTAGTATCTATAATCACTA+GGG | - | chr6.4:50321761-50321780 | None:intergenic | 30.0% | |
| GTACTAGTAATACCTAACTA+AGG | - | chr6.4:50323291-50323310 | None:intergenic | 30.0% | |
| TACGGTGTTCATATTCATAT+TGG | + | chr6.4:50322478-50322497 | MS.gene27400:CDS | 30.0% | |
| TGCAAATACAGATTTGAACT+TGG | - | chr6.4:50322304-50322323 | None:intergenic | 30.0% | |
| TGCTTCAATTCTCTTATTAG+TGG | + | chr6.4:50320570-50320589 | MS.gene27400:intron | 30.0% | |
| TGGTAATTTGGCAAGTAAAT+TGG | - | chr6.4:50321365-50321384 | None:intergenic | 30.0% | |
| TGGTTATATCATCAAAGAGA+TGG | - | chr6.4:50323344-50323363 | None:intergenic | 30.0% | |
| TGTAGATTGTGTAAATTCTG+TGG | - | chr6.4:50323094-50323113 | None:intergenic | 30.0% | |
| TTAGCTAACTAAATGGTATC+CGG | - | chr6.4:50321023-50321042 | None:intergenic | 30.0% | |
| TTATATATTTGCCAGTGTCT+GGG | + | chr6.4:50323067-50323086 | MS.gene27400:CDS | 30.0% | |
| TTGTCGTTATAGTTCTCATA+TGG | + | chr6.4:50322853-50322872 | MS.gene27400:CDS | 30.0% | |
| TTTCAATTAAACTAGCATCG+TGG | - | chr6.4:50324132-50324151 | None:intergenic | 30.0% | |
| TTTCTATACTGTGAAATGGA+AGG | + | chr6.4:50321902-50321921 | MS.gene27400:CDS | 30.0% | |
| ! | AATTGATAATCGAAAACCAG+TGG | - | chr6.4:50322001-50322020 | None:intergenic | 30.0% |
| ! | TATGATGATTTGCCTATTTG+GGG | + | chr6.4:50321702-50321721 | MS.gene27400:CDS | 30.0% |
| ! | TTTATGCATTTTGTGCAGTA+CGG | + | chr6.4:50322460-50322479 | MS.gene27400:intron | 30.0% |
| !! | AAGGTTTTTCGATGTTTTAG+TGG | - | chr6.4:50320885-50320904 | None:intergenic | 30.0% |
| !! | ATGCAAACCTCATTTTTCTT+TGG | + | chr6.4:50324286-50324305 | MS.gene27400:CDS | 30.0% |
| !! | ATGTTGCTATTGTTTTGTTG+CGG | + | chr6.4:50320791-50320810 | MS.gene27400:intron | 30.0% |
| !! | CTCATTTTTCTTTGGATACA+TGG | + | chr6.4:50324294-50324313 | MS.gene27400:CDS | 30.0% |
| AACCAACCTGGTATACAAAA+TGG | - | chr6.4:50320546-50320565 | None:intergenic | 35.0% | |
| ACCTGTTTAGAAGTCAAACA+AGG | - | chr6.4:50322203-50322222 | None:intergenic | 35.0% | |
| ATAAAGAAAGACACAAAGCG+GGG | - | chr6.4:50321233-50321252 | None:intergenic | 35.0% | |
| ATCAGATGAATCAGATCTGA+CGG | - | chr6.4:50320054-50320073 | None:intergenic | 35.0% | |
| ATGCATACAATTGTTCCTTC+AGG | + | chr6.4:50323715-50323734 | MS.gene27400:intron | 35.0% | |
| CAAAATGGATGTGCTTGTAA+AGG | - | chr6.4:50321782-50321801 | None:intergenic | 35.0% | |
| CATGCAAATCTAACTGATAC+TGG | - | chr6.4:50322668-50322687 | None:intergenic | 35.0% | |
| CCACAAAGTAATCACTAAAC+AGG | - | chr6.4:50323517-50323536 | None:intergenic | 35.0% | |
| CCTGTTTAGTGATTACTTTG+TGG | + | chr6.4:50323514-50323533 | MS.gene27400:intron | 35.0% | |
| CGGAAAATTGCGGTTAAATA+TGG | + | chr6.4:50320811-50320830 | MS.gene27400:intron | 35.0% | |
| CGTATTCCAACTTCACATAA+TGG | + | chr6.4:50321086-50321105 | MS.gene27400:intron | 35.0% | |
| GAAATCAGTTCACTGCTAAA+TGG | - | chr6.4:50323313-50323332 | None:intergenic | 35.0% | |
| GACACTTACAAAAGAAGAAG+TGG | + | chr6.4:50321632-50321651 | MS.gene27400:CDS | 35.0% | |
| GCAGGATTTAGCTAACTAAA+TGG | - | chr6.4:50321030-50321049 | None:intergenic | 35.0% | |
| GTTATATATGCACTCACTTC+AGG | + | chr6.4:50322557-50322576 | MS.gene27400:CDS | 35.0% | |
| TACCTCATTCTATTGTCAAC+TGG | + | chr6.4:50322598-50322617 | MS.gene27400:CDS | 35.0% | |
| TATATATTTGCCAGTGTCTG+GGG | + | chr6.4:50323068-50323087 | MS.gene27400:CDS | 35.0% | |
| TATTGAGATCAATGTGCGAA+CGG | + | chr6.4:50321827-50321846 | MS.gene27400:CDS | 35.0% | |
| TCATTCTATTGTCAACTGGA+AGG | + | chr6.4:50322602-50322621 | MS.gene27400:CDS | 35.0% | |
| TCTGATAAGCGTTACAAAGA+AGG | + | chr6.4:50320068-50320087 | MS.gene27400:CDS | 35.0% | |
| TGAAGTCCATTATGTGAAGT+TGG | - | chr6.4:50321095-50321114 | None:intergenic | 35.0% | |
| TGTTACTGCTTTCATTACTG+TGG | + | chr6.4:50323148-50323167 | MS.gene27400:CDS | 35.0% | |
| TGTTTATAGTCCCCTTAGTT+AGG | + | chr6.4:50323276-50323295 | MS.gene27400:intron | 35.0% | |
| TTAGCTACGTATTAGCATAG+TGG | - | chr6.4:50321270-50321289 | None:intergenic | 35.0% | |
| TTCCAGTTGACAATAGAATG+AGG | - | chr6.4:50322603-50322622 | None:intergenic | 35.0% | |
| TTGAAGCAAAAAAACCAACC+TGG | - | chr6.4:50320558-50320577 | None:intergenic | 35.0% | |
| TTGCATGAAACCAGACATAT+CGG | - | chr6.4:50324272-50324291 | None:intergenic | 35.0% | |
| ! | AAGAAGAAGTGGCGAATTTT+CGG | + | chr6.4:50321643-50321662 | MS.gene27400:CDS | 35.0% |
| ! | GATATTTTATGTGGAATCCC+GGG | + | chr6.4:50321181-50321200 | MS.gene27400:intron | 35.0% |
| ! | GGATATTTTATGTGGAATCC+CGG | + | chr6.4:50321180-50321199 | MS.gene27400:intron | 35.0% |
| ! | GTAAGGAAATAAGAGCACAA+AGG | - | chr6.4:50324176-50324195 | None:intergenic | 35.0% |
| ! | TATTTTATCCGTACAACCGA+GGG | + | chr6.4:50322513-50322532 | MS.gene27400:CDS | 35.0% |
| ! | TCATCAGAACTCATTTTCAG+TGG | - | chr6.4:50323448-50323467 | None:intergenic | 35.0% |
| ! | TCCTTGTTTGACTTCTAAAC+AGG | + | chr6.4:50322199-50322218 | MS.gene27400:intron | 35.0% |
| ! | TCGATGTAAATAGCACTGAA+AGG | - | chr6.4:50323041-50323060 | None:intergenic | 35.0% |
| ! | TGATTTTCAGTGAGACGTAT+CGG | + | chr6.4:50320508-50320527 | MS.gene27400:intron | 35.0% |
| ! | TTGGTCCTTTTCATAATCCA+AGG | + | chr6.4:50320120-50320139 | MS.gene27400:CDS | 35.0% |
| ! | TTTGGTTTCTTCTTGATGCT+TGG | + | chr6.4:50324328-50324347 | MS.gene27400:CDS | 35.0% |
| !! | AAAGAGAAGAAAGAAGCACT+GGG | + | chr6.4:50321522-50321541 | MS.gene27400:CDS | 35.0% |
| !! | GTGGAAAGTGTTAATGTTAG+AGG | - | chr6.4:50323429-50323448 | None:intergenic | 35.0% |
| !! | TAATATACAAAGCACCGACA+CGG | + | chr6.4:50320298-50320317 | MS.gene27400:intron | 35.0% |
| !! | TGTTTTGTTGCGGAAAATTG+CGG | + | chr6.4:50320801-50320820 | MS.gene27400:intron | 35.0% |
| !! | TTTCATAGGGAAGGTTGATA+AGG | + | chr6.4:50321725-50321744 | MS.gene27400:CDS | 35.0% |
| AAAAGACAAGGACTCTGTGT+CGG | + | chr6.4:50321599-50321618 | MS.gene27400:CDS | 40.0% | |
| AAACAGGTATGCACATGATG+AGG | + | chr6.4:50322215-50322234 | MS.gene27400:intron | 40.0% | |
| AAACTCACAGGGTAAACAGT+TGG | - | chr6.4:50322347-50322366 | None:intergenic | 40.0% | |
| AACTCACAGGGTAAACAGTT+GGG | - | chr6.4:50322346-50322365 | None:intergenic | 40.0% | |
| AAGAAGGCGAGGATGAATAA+TGG | - | chr6.4:50320005-50320024 | None:intergenic | 40.0% | |
| ACGACTCTAACTCATGAACA+AGG | + | chr6.4:50323921-50323940 | MS.gene27400:intron | 40.0% | |
| ACGTCTCCTCTACTTGTATT+GGG | + | chr6.4:50322885-50322904 | MS.gene27400:CDS | 40.0% | |
| AGCACAAAGGTTAAATGTGC+AGG | - | chr6.4:50324163-50324182 | None:intergenic | 40.0% | |
| ATAACGACAATTGTGCCGAA+TGG | - | chr6.4:50322843-50322862 | None:intergenic | 40.0% | |
| ATTGATCGAACATCGCATCA+AGG | - | chr6.4:50320904-50320923 | None:intergenic | 40.0% | |
| CCAACATCGATCAACTACAT+TGG | - | chr6.4:50323364-50323383 | None:intergenic | 40.0% | |
| CCAATGTAGTTGATCGATGT+TGG | + | chr6.4:50323361-50323380 | MS.gene27400:intron | 40.0% | |
| CCCTTGGATATTTAGTTGTG+CGG | - | chr6.4:50322956-50322975 | None:intergenic | 40.0% | |
| CCGCACAACTAAATATCCAA+GGG | + | chr6.4:50322953-50322972 | MS.gene27400:CDS | 40.0% | |
| CCTTACAGATCATTCCTATG+CGG | + | chr6.4:50324190-50324209 | MS.gene27400:intron | 40.0% | |
| CGACTCTAACTCATGAACAA+GGG | + | chr6.4:50323922-50323941 | MS.gene27400:intron | 40.0% | |
| CGTGGAATGCATGTGAATAA+CGG | - | chr6.4:50320158-50320177 | None:intergenic | 40.0% | |
| CTAGACCATCCAATCTTGAT+CGG | - | chr6.4:50321312-50321331 | None:intergenic | 40.0% | |
| CTTTGTCCGTTTATCATGAC+AGG | + | chr6.4:50322713-50322732 | MS.gene27400:intron | 40.0% | |
| GAAACCTAAAAACATCACCG+TGG | - | chr6.4:50322281-50322300 | None:intergenic | 40.0% | |
| GAAGAAACCAAAGCATATGC+AGG | - | chr6.4:50324320-50324339 | None:intergenic | 40.0% | |
| GAGAGAAGAAATGGAGAAGA+AGG | - | chr6.4:50320021-50320040 | None:intergenic | 40.0% | |
| GGAGATGAAGAGAGAAGAAA+TGG | - | chr6.4:50320030-50320049 | None:intergenic | 40.0% | |
| GGGAAACATTCTTGCTTTCA+TGG | + | chr6.4:50323942-50323961 | MS.gene27400:intron | 40.0% | |
| GTTCGGAATCTACTATTGAC+CGG | + | chr6.4:50322735-50322754 | MS.gene27400:CDS | 40.0% | |
| TAAAGAAAGACACAAAGCGG+GGG | - | chr6.4:50321232-50321251 | None:intergenic | 40.0% | |
| TAACGACAATTGTGCCGAAT+GGG | - | chr6.4:50322842-50322861 | None:intergenic | 40.0% | |
| TACGTCTCCTCTACTTGTAT+TGG | + | chr6.4:50322884-50322903 | MS.gene27400:CDS | 40.0% | |
| TCAACTGGAAGGAACAAACT+GGG | + | chr6.4:50322613-50322632 | MS.gene27400:CDS | 40.0% | |
| TCATGTAACGGTGTAAATGC+AGG | - | chr6.4:50321048-50321067 | None:intergenic | 40.0% | |
| TCCGAACCTGTCATGATAAA+CGG | - | chr6.4:50322722-50322741 | None:intergenic | 40.0% | |
| TCCGCACAACTAAATATCCA+AGG | + | chr6.4:50322952-50322971 | MS.gene27400:CDS | 40.0% | |
| TCCGTTTATCATGACAGGTT+CGG | + | chr6.4:50322718-50322737 | MS.gene27400:intron | 40.0% | |
| TGCTGATGCAATTGTAGTTG+TGG | + | chr6.4:50320838-50320857 | MS.gene27400:intron | 40.0% | |
| TTCCCTATGAAACCCCAAAT+AGG | - | chr6.4:50321717-50321736 | None:intergenic | 40.0% | |
| TTCTTGATGCTTGGAACTGT+TGG | + | chr6.4:50324337-50324356 | MS.gene27400:CDS | 40.0% | |
| TTGCAAGAAAACCAGTCAAG+AGG | - | chr6.4:50322040-50322059 | None:intergenic | 40.0% | |
| TTGCTGCTGAAGATCATGAA+TGG | + | chr6.4:50323189-50323208 | MS.gene27400:CDS | 40.0% | |
| TTGTAGTTGTGGAGACATCA+TGG | + | chr6.4:50320849-50320868 | MS.gene27400:intron | 40.0% | |
| TTTACCCTGTGAGTTTCTTC+TGG | + | chr6.4:50322351-50322370 | MS.gene27400:intron | 40.0% | |
| ! | ACACAGAGTCCTTGTCTTTT+TGG | - | chr6.4:50321599-50321618 | None:intergenic | 40.0% |
| ! | ACATCCACGGTGATGTTTTT+AGG | + | chr6.4:50322274-50322293 | MS.gene27400:CDS | 40.0% |
| ! | ACCAGTGGATTTCCAAATGA+TGG | - | chr6.4:50321986-50322005 | None:intergenic | 40.0% |
| ! | ACTTGATGGATCGGTATATG+TGG | - | chr6.4:50324390-50324409 | None:intergenic | 40.0% |
| ! | ATTTTATCCGTACAACCGAG+GGG | + | chr6.4:50322514-50322533 | MS.gene27400:CDS | 40.0% |
| ! | CAGTGGATTTCCAAATGATG+GGG | - | chr6.4:50321984-50322003 | None:intergenic | 40.0% |
| ! | CCAGTGGATTTCCAAATGAT+GGG | - | chr6.4:50321985-50322004 | None:intergenic | 40.0% |
| ! | GAAAGAGAAGAAAGAAGCAC+TGG | + | chr6.4:50321521-50321540 | MS.gene27400:CDS | 40.0% |
| ! | GTATTTTATCCGTACAACCG+AGG | + | chr6.4:50322512-50322531 | MS.gene27400:CDS | 40.0% |
| ! | TATTTGGGGTTTCATAGGGA+AGG | + | chr6.4:50321716-50321735 | MS.gene27400:CDS | 40.0% |
| ! | TGCCTATTTGGGGTTTCATA+GGG | + | chr6.4:50321712-50321731 | MS.gene27400:CDS | 40.0% |
| ! | TTGCCTATTTGGGGTTTCAT+AGG | + | chr6.4:50321711-50321730 | MS.gene27400:CDS | 40.0% |
| !! | ATAGGGAAGGTTGATAAGGA+GGG | + | chr6.4:50321729-50321748 | MS.gene27400:CDS | 40.0% |
| !!! | GATCTGCCATTTTGTATACC+AGG | + | chr6.4:50320537-50320556 | MS.gene27400:CDS | 40.0% |
| !!! | GGCTCTGAGGATATTTTATG+TGG | + | chr6.4:50321172-50321191 | MS.gene27400:intron | 40.0% |
| !!! | TGCCATTTTGTATACCAGGT+TGG | + | chr6.4:50320541-50320560 | MS.gene27400:intron | 40.0% |
| !!! | TTTTGGAACTCGAGCTGATA+AGG | - | chr6.4:50321582-50321601 | None:intergenic | 40.0% |
| AAAGAAAGACACAAAGCGGG+GGG | - | chr6.4:50321231-50321250 | None:intergenic | 45.0% | |
| AAGAAATGGAGAAGAAGGCG+AGG | - | chr6.4:50320016-50320035 | None:intergenic | 45.0% | |
| AATAGACTCTCGAGTCCTGA+AGG | - | chr6.4:50323733-50323752 | None:intergenic | 45.0% | |
| AATGGCCAGAAGAAACTCAC+AGG | - | chr6.4:50322359-50322378 | None:intergenic | 45.0% | |
| ACACCGACACATCTGATCAA+AGG | - | chr6.4:50320424-50320443 | None:intergenic | 45.0% | |
| ACCATCCAATCTTGATCGGA+TGG | - | chr6.4:50321308-50321327 | None:intergenic | 45.0% | |
| ACCATCCGATCAAGATTGGA+TGG | + | chr6.4:50321304-50321323 | MS.gene27400:intron | 45.0% | |
| ACGCCTTTGATCAGATGTGT+CGG | + | chr6.4:50320418-50320437 | MS.gene27400:intron | 45.0% | |
| ACTGGCTGGAAATACATCCA+CGG | + | chr6.4:50322261-50322280 | MS.gene27400:CDS | 45.0% | |
| ACTGTTGCATCCTAGTGGTT+TGG | + | chr6.4:50321151-50321170 | MS.gene27400:intron | 45.0% | |
| AGATGAATCAGATCTGACGG+TGG | - | chr6.4:50320051-50320070 | None:intergenic | 45.0% | |
| AGCAATTCCTCAGATGGCAA+TGG | + | chr6.4:50323004-50323023 | MS.gene27400:CDS | 45.0% | |
| ATCCCTCCCAATACAAGTAG+AGG | - | chr6.4:50322894-50322913 | None:intergenic | 45.0% | |
| ATGGACCATCCGATCAAGAT+TGG | + | chr6.4:50321300-50321319 | MS.gene27400:intron | 45.0% | |
| ATGGCCAGAAGAAACTCACA+GGG | - | chr6.4:50322358-50322377 | None:intergenic | 45.0% | |
| CAAGAAAACCAGTCAAGAGG+AGG | - | chr6.4:50322037-50322056 | None:intergenic | 45.0% | |
| CCAACTTTGTTAGCGAAGAG+AGG | - | chr6.4:50320104-50320123 | None:intergenic | 45.0% | |
| CCCATCATTTGGAAATCCAC+TGG | + | chr6.4:50321982-50322001 | MS.gene27400:CDS | 45.0% | |
| CCGCATAGGAATGATCTGTA+AGG | - | chr6.4:50324193-50324212 | None:intergenic | 45.0% | |
| CCTCTCTTCGCTAACAAAGT+TGG | + | chr6.4:50320101-50320120 | MS.gene27400:CDS | 45.0% | |
| CTCCTCTACTTGTATTGGGA+GGG | + | chr6.4:50322889-50322908 | MS.gene27400:CDS | 45.0% | |
| CTGAAGATCATGAATGGTGG+TGG | + | chr6.4:50323195-50323214 | MS.gene27400:CDS | 45.0% | |
| CTGCTGAAGATCATGAATGG+TGG | + | chr6.4:50323192-50323211 | MS.gene27400:CDS | 45.0% | |
| GAAATAGAGGCCCACAGAAA+AGG | - | chr6.4:50322764-50322783 | None:intergenic | 45.0% | |
| GCTCGAGTTCCAAAAAGACA+AGG | + | chr6.4:50321587-50321606 | MS.gene27400:CDS | 45.0% | |
| GCTTAGGGGTGATGCATTTA+TGG | + | chr6.4:50323647-50323666 | MS.gene27400:intron | 45.0% | |
| GGACAAAGAACAATGCTGCA+CGG | - | chr6.4:50324366-50324385 | None:intergenic | 45.0% | |
| GGATATTTAGTTGTGCGGAC+TGG | - | chr6.4:50322951-50322970 | None:intergenic | 45.0% | |
| GGTGCAACCAAGGAAATTCT+CGG | + | chr6.4:50323995-50324014 | MS.gene27400:intron | 45.0% | |
| GTAGTAGTCCTTACGAACAG+CGG | - | chr6.4:50321670-50321689 | None:intergenic | 45.0% | |
| GTCAACTGGAAGGAACAAAC+TGG | + | chr6.4:50322612-50322631 | MS.gene27400:CDS | 45.0% | |
| GTCCTCTGTAACATCCACAA+GGG | - | chr6.4:50321862-50321881 | None:intergenic | 45.0% | |
| GTGCAACCAAGGAAATTCTC+GGG | + | chr6.4:50323996-50324015 | MS.gene27400:intron | 45.0% | |
| TACAGATCATTCCTATGCGG+TGG | + | chr6.4:50324193-50324212 | MS.gene27400:intron | 45.0% | |
| TACATGGCCTGCATATGCTT+TGG | + | chr6.4:50324310-50324329 | MS.gene27400:CDS | 45.0% | |
| TCTCCTCTACTTGTATTGGG+AGG | + | chr6.4:50322888-50322907 | MS.gene27400:CDS | 45.0% | |
| TCTGTATTTGCAGCAGCTCT+TGG | + | chr6.4:50322312-50322331 | MS.gene27400:CDS | 45.0% | |
| TGAAACCAGACATATCGGAG+CGG | - | chr6.4:50324267-50324286 | None:intergenic | 45.0% | |
| TGCAATCCCGAGAATTTCCT+TGG | - | chr6.4:50324005-50324024 | None:intergenic | 45.0% | |
| TGTCCTCTGTAACATCCACA+AGG | - | chr6.4:50321863-50321882 | None:intergenic | 45.0% | |
| TTCCTCCTTTGCCATGGTAT+CGG | + | chr6.4:50322979-50322998 | MS.gene27400:CDS | 45.0% | |
| TTGTATTGGGAGGGATTGCT+GGG | + | chr6.4:50322898-50322917 | MS.gene27400:CDS | 45.0% | |
| TTTGGCAAGTAAATTGGCCC+CGG | - | chr6.4:50321359-50321378 | None:intergenic | 45.0% | |
| ! | AGTGTTGAATGGGGATAGGT+TGG | + | chr6.4:50321548-50321567 | MS.gene27400:CDS | 45.0% |
| ! | CATCTGATCAAAGGCGTATC+CGG | - | chr6.4:50320415-50320434 | None:intergenic | 45.0% |
| ! | TGATGGATCGGTATATGTGG+CGG | - | chr6.4:50324387-50324406 | None:intergenic | 45.0% |
| !! | AACGGATCGAAGAAGTACCT+TGG | - | chr6.4:50320140-50320159 | None:intergenic | 45.0% |
| !! | AAGATCATGAATGGTGGTGG+AGG | + | chr6.4:50323198-50323217 | MS.gene27400:CDS | 45.0% |
| !! | ACTGGGTGAAGTGTTGAATG+GGG | + | chr6.4:50321539-50321558 | MS.gene27400:CDS | 45.0% |
| !! | CACTGGGTGAAGTGTTGAAT+GGG | + | chr6.4:50321538-50321557 | MS.gene27400:CDS | 45.0% |
| !! | CATAGGGAAGGTTGATAAGG+AGG | + | chr6.4:50321728-50321747 | MS.gene27400:CDS | 45.0% |
| !! | GTAACATCCACAAGGGCATT+TGG | - | chr6.4:50321855-50321874 | None:intergenic | 45.0% |
| !! | GTGAAGTGTTGAATGGGGAT+AGG | + | chr6.4:50321544-50321563 | MS.gene27400:CDS | 45.0% |
| !! | TAGGGAAGGTTGATAAGGAG+GGG | + | chr6.4:50321730-50321749 | MS.gene27400:CDS | 45.0% |
| AACAACGTGATTGCGTGACC+GGG | + | chr6.4:50321338-50321357 | MS.gene27400:intron | 50.0% | |
| AACGGATCCAAATGCCCTTG+TGG | + | chr6.4:50321845-50321864 | MS.gene27400:CDS | 50.0% | |
| AAGAAAGACACAAAGCGGGG+GGG | - | chr6.4:50321230-50321249 | None:intergenic | 50.0% | |
| AATAGAGCCCCTCGGTTGTA+CGG | - | chr6.4:50322524-50322543 | None:intergenic | 50.0% | |
| AATCCTGCCATTGCCATCTG+AGG | - | chr6.4:50323014-50323033 | None:intergenic | 50.0% | |
| AATTGTGCCGAATGGGAGAG+CGG | - | chr6.4:50322835-50322854 | None:intergenic | 50.0% | |
| ACATGATGAGGAGACTGCAG+AGG | + | chr6.4:50322227-50322246 | MS.gene27400:CDS | 50.0% | |
| ACCACTAGGATGCAACAGTG+AGG | - | chr6.4:50321150-50321169 | None:intergenic | 50.0% | |
| ACGAAAACAGATCCAGCACG+TGG | - | chr6.4:50320176-50320195 | None:intergenic | 50.0% | |
| ATCCAGTTGAACCACCGCAT+AGG | - | chr6.4:50324207-50324226 | None:intergenic | 50.0% | |
| ATCCTCAGAGCCAAACCACT+AGG | - | chr6.4:50321164-50321183 | None:intergenic | 50.0% | |
| ATTCCTCAGATGGCAATGGC+AGG | + | chr6.4:50323008-50323027 | MS.gene27400:CDS | 50.0% | |
| CAACAACGTGATTGCGTGAC+CGG | + | chr6.4:50321337-50321356 | MS.gene27400:intron | 50.0% | |
| CATGATCTTCAGCAGCAAGC+TGG | - | chr6.4:50323187-50323206 | None:intergenic | 50.0% | |
| CTAACACTCCCTTCGAGAAG+CGG | + | chr6.4:50321928-50321947 | MS.gene27400:CDS | 50.0% | |
| CTTGTATTGGGAGGGATTGC+TGG | + | chr6.4:50322897-50322916 | MS.gene27400:CDS | 50.0% | |
| GAAACCAGACATATCGGAGC+GGG | - | chr6.4:50324266-50324285 | None:intergenic | 50.0% | |
| GATCCATCAAGTGCGAGTAG+TGG | + | chr6.4:50324398-50324417 | MS.gene27400:CDS | 50.0% | |
| GATTGCTGGGAAGAACAGCA+AGG | + | chr6.4:50322911-50322930 | MS.gene27400:CDS | 50.0% | |
| TCCTCACTGTTGCATCCTAG+TGG | + | chr6.4:50321146-50321165 | MS.gene27400:intron | 50.0% | |
| TCCTCCTTTGCCATGGTATC+GGG | + | chr6.4:50322980-50322999 | MS.gene27400:CDS | 50.0% | |
| TGCCCTTGTGGATGTTACAG+AGG | + | chr6.4:50321857-50321876 | MS.gene27400:CDS | 50.0% | |
| TTCACATGCATTCCACGTGC+TGG | + | chr6.4:50320161-50320180 | MS.gene27400:intron | 50.0% | |
| TTCCTATGCGGTGGTTCAAC+TGG | + | chr6.4:50324202-50324221 | MS.gene27400:CDS | 50.0% | |
| TTGTCCATCCGCTTCTCGAA+GGG | - | chr6.4:50321939-50321958 | None:intergenic | 50.0% | |
| TTTGCAGCAGCTCTTGGATC+TGG | + | chr6.4:50322318-50322337 | MS.gene27400:CDS | 50.0% | |
| ! | AAATGATGGGGTAGCGAGGA+CGG | - | chr6.4:50321972-50321991 | None:intergenic | 50.0% |
| ! | ATCAAAGGCGTATCCGGTGT+TGG | - | chr6.4:50320409-50320428 | None:intergenic | 50.0% |
| ! | TGACCGGATGCCTTTTCTGT+GGG | + | chr6.4:50322751-50322770 | MS.gene27400:CDS | 50.0% |
| ! | TTCCAAATGATGGGGTAGCG+AGG | - | chr6.4:50321976-50321995 | None:intergenic | 50.0% |
| ! | TTGACCGGATGCCTTTTCTG+TGG | + | chr6.4:50322750-50322769 | MS.gene27400:CDS | 50.0% |
| ! | TTTTCGGTCCGCTGTTCGTA+AGG | + | chr6.4:50321659-50321678 | MS.gene27400:CDS | 50.0% |
| !! | ATCCTAGTGGTTTGGCTCTG+AGG | + | chr6.4:50321159-50321178 | MS.gene27400:intron | 50.0% |
| !! | GCACTGGGTGAAGTGTTGAA+TGG | + | chr6.4:50321537-50321556 | MS.gene27400:CDS | 50.0% |
| AATTCTGTGGCCCCAGACAC+TGG | - | chr6.4:50323081-50323100 | None:intergenic | 55.0% | |
| ACAACGTGATTGCGTGACCG+GGG | + | chr6.4:50321339-50321358 | MS.gene27400:intron | 55.0% | |
| AGGATCAGGAAGAGACTGGC+TGG | + | chr6.4:50322247-50322266 | MS.gene27400:CDS | 55.0% | |
| CCTCCTTTGCCATGGTATCG+GGG | + | chr6.4:50322981-50323000 | MS.gene27400:CDS | 55.0% | |
| CTTGTCCATCCGCTTCTCGA+AGG | - | chr6.4:50321940-50321959 | None:intergenic | 55.0% | |
| GCACTCACTTCAGGAATCGC+TGG | + | chr6.4:50322566-50322585 | MS.gene27400:CDS | 55.0% | |
| GCAGAGGATCAGGAAGAGAC+TGG | + | chr6.4:50322243-50322262 | MS.gene27400:CDS | 55.0% | |
| GGAATTGCTCCCCGATACCA+TGG | - | chr6.4:50322993-50323012 | None:intergenic | 55.0% | |
| GGCAAAGGAGGAATCTCCCT+TGG | - | chr6.4:50322972-50322991 | None:intergenic | 55.0% | |
| GGGAGATTCCTCCTTTGCCA+TGG | + | chr6.4:50322973-50322992 | MS.gene27400:CDS | 55.0% | |
| GTCACAGTCCTCCTCTTGAC+TGG | + | chr6.4:50322026-50322045 | MS.gene27400:CDS | 55.0% | |
| GTCCTCGCTACCCCATCATT+TGG | + | chr6.4:50321971-50321990 | MS.gene27400:CDS | 55.0% | |
| GTGCAGTGAATAGAGCCCCT+CGG | - | chr6.4:50322532-50322551 | None:intergenic | 55.0% | |
| TATGCCCGCTCCGATATGTC+TGG | + | chr6.4:50324259-50324278 | MS.gene27400:CDS | 55.0% | |
| TCCAGTGTCTGTGTCCGTGT+CGG | - | chr6.4:50320315-50320334 | None:intergenic | 55.0% | |
| TCGGGGAGCAATTCCTCAGA+TGG | + | chr6.4:50322998-50323017 | MS.gene27400:CDS | 55.0% | |
| TGAGGAGACTGCAGAGGATC+AGG | + | chr6.4:50322233-50322252 | MS.gene27400:CDS | 55.0% | |
| TGGTGACTCAGGTTCGAACC+CGG | - | chr6.4:50321202-50321221 | None:intergenic | 55.0% | |
| !! | AGGCCCACAGAAAAGGCATC+CGG | - | chr6.4:50322757-50322776 | None:intergenic | 55.0% |
| !! | TAGTTGTGCGGACTGGAGCT+TGG | - | chr6.4:50322944-50322963 | None:intergenic | 55.0% |
| ACCGACACGGACACAGACAC+TGG | + | chr6.4:50320311-50320330 | MS.gene27400:intron | 60.0% | |
| CACAAAGCGGGGGGGAAATG+TGG | - | chr6.4:50321222-50321241 | None:intergenic | 60.0% | |
| CACTCCCTTCGAGAAGCGGA+TGG | + | chr6.4:50321932-50321951 | MS.gene27400:CDS | 60.0% | |
| CCCCGATACCATGGCAAAGG+AGG | - | chr6.4:50322984-50323003 | None:intergenic | 60.0% | |
| GCTACTGCCGCTCTCCCATT+CGG | + | chr6.4:50322825-50322844 | MS.gene27400:CDS | 60.0% | |
| GCTCCCCGATACCATGGCAA+AGG | - | chr6.4:50322987-50323006 | None:intergenic | 60.0% | |
| GGGGGGAAATGTGGTGACTC+AGG | - | chr6.4:50321213-50321232 | None:intergenic | 60.0% | |
| GGTGACTCAGGTTCGAACCC+GGG | - | chr6.4:50321201-50321220 | None:intergenic | 60.0% | |
| GTCGTGTCCGTGTCCAACAC+CGG | + | chr6.4:50320393-50320412 | MS.gene27400:intron | 60.0% | |
| ! | GGCGTATCCGGTGTTGGACA+CGG | - | chr6.4:50320403-50320422 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 50319987 | 50324417 | 50319987 | ID=MS.gene27400 |
| chr6.4 | mRNA | 50319987 | 50324417 | 50319987 | ID=MS.gene27400.t1;Parent=MS.gene27400 |
| chr6.4 | exon | 50319987 | 50320141 | 50319987 | ID=MS.gene27400.t1.exon1;Parent=MS.gene27400.t1 |
| chr6.4 | CDS | 50319987 | 50320141 | 50319987 | ID=cds.MS.gene27400.t1;Parent=MS.gene27400.t1 |
| chr6.4 | exon | 50320518 | 50320558 | 50320518 | ID=MS.gene27400.t1.exon2;Parent=MS.gene27400.t1 |
| chr6.4 | CDS | 50320518 | 50320558 | 50320518 | ID=cds.MS.gene27400.t1;Parent=MS.gene27400.t1 |
| chr6.4 | exon | 50321514 | 50322096 | 50321514 | ID=MS.gene27400.t1.exon3;Parent=MS.gene27400.t1 |
| chr6.4 | CDS | 50321514 | 50322096 | 50321514 | ID=cds.MS.gene27400.t1;Parent=MS.gene27400.t1 |
| chr6.4 | exon | 50322221 | 50322358 | 50322221 | ID=MS.gene27400.t1.exon4;Parent=MS.gene27400.t1 |
| chr6.4 | CDS | 50322221 | 50322358 | 50322221 | ID=cds.MS.gene27400.t1;Parent=MS.gene27400.t1 |
| chr6.4 | exon | 50322478 | 50322634 | 50322478 | ID=MS.gene27400.t1.exon5;Parent=MS.gene27400.t1 |
| chr6.4 | CDS | 50322478 | 50322634 | 50322478 | ID=cds.MS.gene27400.t1;Parent=MS.gene27400.t1 |
| chr6.4 | exon | 50322735 | 50323219 | 50322735 | ID=MS.gene27400.t1.exon6;Parent=MS.gene27400.t1 |
| chr6.4 | CDS | 50322735 | 50323219 | 50322735 | ID=cds.MS.gene27400.t1;Parent=MS.gene27400.t1 |
| chr6.4 | exon | 50324198 | 50324417 | 50324198 | ID=MS.gene27400.t1.exon7;Parent=MS.gene27400.t1 |
| chr6.4 | CDS | 50324198 | 50324417 | 50324198 | ID=cds.MS.gene27400.t1;Parent=MS.gene27400.t1 |
| Gene Sequence |
| Protein sequence |
