AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27787


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27787.t1 MTR_1g017090 95.726 117 4 1 37 152 155 271 2.30e-76 229
MS.gene27787.t1 MTR_5g014890 62.295 122 35 3 37 153 148 263 4.77e-45 149
MS.gene27787.t1 MTR_4g097600 74.444 90 21 1 37 126 152 239 2.00e-42 142
MS.gene27787.t1 MTR_1g054285 71.429 91 24 1 37 127 179 267 2.43e-41 140
MS.gene27787.t1 MTR_7g093430 71.429 91 24 1 37 127 185 273 3.25e-41 139
MS.gene27787.t1 MTR_2g061030 56.349 126 50 3 4 126 189 312 1.83e-39 136
MS.gene27787.t1 MTR_4g100550 57.522 113 38 3 37 148 145 248 4.47e-38 131
MS.gene27787.t1 MTR_5g013010 54.701 117 43 3 37 152 161 268 3.09e-37 129
MS.gene27787.t1 MTR_8g006705 67.416 89 26 2 37 124 171 257 5.73e-36 126
MS.gene27787.t1 MTR_3g103590 59.350 123 40 3 37 152 170 289 1.04e-33 120
MS.gene27787.t1 MTR_4g084750 54.455 101 41 2 29 124 81 181 7.02e-29 105
MS.gene27787.t1 MTR_2g038625 56.989 93 39 1 38 130 108 199 4.11e-26 98.6
MS.gene27787.t1 MTR_2g038580 56.989 93 39 1 38 130 108 199 4.11e-26 98.6
MS.gene27787.t1 MTR_4g126900 57.303 89 36 2 38 124 114 202 3.11e-24 94.0
MS.gene27787.t1 MTR_7g010020 50.000 70 28 1 37 99 105 174 2.65e-12 63.2
MS.gene27787.t1 MTR_6g007647 52.381 63 30 0 37 99 92 154 3.02e-12 62.8
MS.gene27787.t1 MTR_6g007647 52.381 63 30 0 37 99 58 120 3.45e-12 62.4
MS.gene27787.t1 MTR_5g039000 47.619 63 33 0 37 99 102 164 1.18e-11 61.2
MS.gene27787.t1 MTR_3g086790 36.441 118 65 2 37 147 85 199 1.77e-11 60.8
MS.gene27787.t1 MTR_3g080100 47.143 70 30 1 37 99 45 114 4.26e-11 58.9
MS.gene27787.t1 MTR_6g011610 50.725 69 27 1 37 98 121 189 5.08e-11 59.3
MS.gene27787.t1 MTR_8g468210 44.444 63 35 0 37 99 102 164 6.30e-11 59.3
MS.gene27787.t1 MTR_3g080100 47.143 70 30 1 37 99 84 153 6.40e-11 58.9
MS.gene27787.t1 MTR_6g011610 50.725 69 27 1 37 98 102 170 8.15e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27787.t1 AT4G37790 73.333 90 22 1 37 126 151 238 2.64e-42 142
MS.gene27787.t1 AT5G06710 65.049 103 31 2 37 139 215 312 1.41e-41 141
MS.gene27787.t1 AT2G22800 71.111 90 24 1 37 126 138 225 3.18e-39 134
MS.gene27787.t1 AT4G16780 56.637 113 39 3 37 148 154 257 1.27e-36 127
MS.gene27787.t1 AT5G47370 45.625 160 61 5 4 151 111 256 1.02e-34 122
MS.gene27787.t1 AT2G44910 64.835 91 29 2 37 126 188 276 1.50e-34 123
MS.gene27787.t1 AT3G60390 65.909 88 29 1 37 124 187 273 1.55e-34 122
MS.gene27787.t1 AT4G17460 60.870 92 35 1 37 128 160 250 9.88e-32 114
MS.gene27787.t1 AT2G01430 51.515 99 43 3 29 126 155 249 7.95e-25 96.7
MS.gene27787.t1 AT1G70920 48.000 100 39 2 37 134 64 152 1.22e-22 88.6
MS.gene27787.t1 AT1G70920 48.000 100 39 2 37 134 94 182 1.77e-22 89.0
MS.gene27787.t1 AT1G26960 41.111 90 49 2 38 127 97 182 1.42e-12 63.5
MS.gene27787.t1 AT5G15150 39.048 105 55 2 37 134 140 242 6.06e-11 59.3

Find 0 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAAAAAATTAAATTCTA+AGG - 8583:27381-27400 MS.gene27787:intergenic 10.0%
!! AAACAAAGAAGTAAAATATA+TGG - 8583:27792-27811 MS.gene27787:intergenic 15.0%
!! AAGTTTATAAAAATTGTTTC+AGG - 8583:27286-27305 MS.gene27787:intergenic 15.0%
!!! ACAATTTTTATAAACTTGTA+CGG + 8583:27289-27308 MS.gene27787:intron 15.0%
!!! ATTTTATTAGAATTAGATCT+TGG + 8583:27076-27095 MS.gene27787:intron 15.0%
!! AAATTTGTAATACATTGGAT+TGG - 8583:27424-27443 MS.gene27787:intergenic 20.0%
!! AGTTTCAAAATTTATTCTCT+TGG + 8583:27157-27176 MS.gene27787:intron 20.0%
!! ATATAATTTCACAAAATCTG+AGG - 8583:28088-28107 MS.gene27787:intergenic 20.0%
!! ATGAGAATATTGATGAATTA+AGG - 8583:26791-26810 MS.gene27787:intergenic 20.0%
!! CTACAAAATTTGTAATACAT+TGG - 8583:27429-27448 MS.gene27787:intergenic 20.0%
!! TTTCAGGAAATTATTTATTC+TGG - 8583:27270-27289 MS.gene27787:intergenic 20.0%
!!! ACTTCTTTGTTTTTTTTTAC+TGG + 8583:27800-27819 MS.gene27787:intron 20.0%
!!! CATTTTTTTAGAAATGTTTC+TGG + 8583:27338-27357 MS.gene27787:intron 20.0%
!!! GAAACATTTCTAAAAAAATG+AGG - 8583:27338-27357 MS.gene27787:intergenic 20.0%
! ACAAAAAATGTTAACAGCTA+GGG + 8583:27994-28013 MS.gene27787:intron 25.0%
! AGACGTTTAAAAAGTTTAGA+AGG - 8583:28035-28054 MS.gene27787:intergenic 25.0%
! ATATATATTGTTAGAGCGAA+AGG + 8583:27903-27922 MS.gene27787:intron 25.0%
! ATATTGATGAATTAAGGTAC+CGG - 8583:26785-26804 MS.gene27787:intergenic 25.0%
! CATCAATATTCTCATATCTT+TGG + 8583:26796-26815 MS.gene27787:intron 25.0%
!! AAAACAACAATCAGAAGTTT+TGG + 8583:26727-26746 MS.gene27787:CDS 25.0%
!! AATATCAAAACAAAAGTACG+TGG - 8583:26851-26870 MS.gene27787:intergenic 25.0%
AAAAGTTTAGAAGGAGGTTT+AGG - 8583:28026-28045 MS.gene27786:intergenic 30.0%
AAAGTTTAGAAGGAGGTTTA+GGG - 8583:28025-28044 MS.gene27786:intergenic 30.0%
ATCATCATGACAATTGCTAA+TGG - 8583:27670-27689 MS.gene27787:intergenic 30.0%
CACAAAAAATGTTAACAGCT+AGG + 8583:27993-28012 MS.gene27787:intron 30.0%
CGTTTAAAAAGTTTAGAAGG+AGG - 8583:28032-28051 MS.gene27787:intergenic 30.0%
GCTGGAATCTTCATATAAAA+GGG - 8583:28279-28298 MS.gene27787:intergenic 30.0%
GGTAAAATCTTGATATTCCT+AGG + 8583:27219-27238 MS.gene27787:intron 30.0%
TATGAAGCAAACTCAATCTA+AGG - 8583:27138-27157 MS.gene27787:intergenic 30.0%
TGCTGGAATCTTCATATAAA+AGG - 8583:28280-28299 MS.gene27787:intergenic 30.0%
TTAGAATTAGATCTTGGTCT+TGG + 8583:27082-27101 MS.gene27787:intron 30.0%
TTCACAAAATCTGAGGAATA+AGG - 8583:28081-28100 MS.gene27787:intergenic 30.0%
! AAAACAAAAGTACGTGGTAT+AGG - 8583:26845-26864 MS.gene27787:intergenic 30.0%
! ATTTTACCAAACACTTTGGT+GGG - 8583:27207-27226 MS.gene27787:intergenic 30.0%
! CAAGATTTTACCAAACACTT+TGG - 8583:27211-27230 MS.gene27787:intergenic 30.0%
! TCTCTTGGATTACATTTTCT+AGG + 8583:27172-27191 MS.gene27787:intron 30.0%
AAGGTAAAGTATGCGATCAA+AGG - 8583:28062-28081 MS.gene27786:intergenic 35.0%
AGGTAAAGTATGCGATCAAA+GGG - 8583:28061-28080 MS.gene27787:intergenic 35.0%
CAGAACAAAGCTGAAACAAA+CGG + 8583:28143-28162 MS.gene27787:intron 35.0%
CGATACTATCTTAAAACGCA+CGG - 8583:27601-27620 MS.gene27787:intergenic 35.0%
CTTGATATTCCTAGGAAAGT+TGG + 8583:27227-27246 MS.gene27787:intron 35.0%
! AGAGCATTCTACTCTTAATC+CGG + 8583:26763-26782 MS.gene27787:CDS 35.0%
! ATATTCCTAGGAAAGTTGGT+AGG + 8583:27231-27250 MS.gene27787:intron 35.0%
! ATTATTCCCACCAAAGTGTT+TGG + 8583:27198-27217 MS.gene27787:intron 35.0%
! GATTTTACCAAACACTTTGG+TGG - 8583:27208-27227 MS.gene27787:intergenic 35.0%
! TAGAGGTGTGGTTTCAAAAT+CGG + 8583:27554-27573 MS.gene27787:CDS 35.0%
! TCTAAGGTGTGTTTGTTTCA+AGG - 8583:27365-27384 MS.gene27787:intergenic 35.0%
!! ACAACAATCAGAAGTTTTGG+AGG + 8583:26730-26749 MS.gene27787:CDS 35.0%
AAGCAATGTTGTTGAGGGTA+AGG - 8583:28361-28380 MS.gene27786:intergenic 40.0%
AGCAAATAAGCTGAATCTGC+AGG + 8583:27522-27541 MS.gene27787:CDS 40.0%
AGGAGAAAACTTAGATGGCA+CGG + 8583:27923-27942 MS.gene27787:intron 40.0%
ATTTGTGGTGGCAACAACAA+TGG + 8583:28333-28352 MS.gene27787:CDS 40.0%
CCAGTAAGCAATGTTGTTGA+GGG - 8583:28366-28385 MS.gene27787:intergenic 40.0%
GCGAAAGGAGAAAACTTAGA+TGG + 8583:27918-27937 MS.gene27787:intron 40.0%
GGAGAACAAGAAGTTGCAAA+AGG + 8583:28212-28231 MS.gene27787:CDS 40.0%
TATTCTCTACGTGCGCAATA+TGG - 8583:27960-27979 MS.gene27787:intergenic 40.0%
TGAGCCTAAGTTTCTTCCTT+GGG - 8583:26706-26725 MS.gene27787:intergenic 40.0%
! CACGCACAGTATTTTTCTCA+TGG + 8583:26978-26997 MS.gene27787:intron 40.0%
! TGGTCTTGGTTGATTGTGTT+TGG + 8583:27096-27115 MS.gene27787:intron 40.0%
ACACACCTACCAACTTTCCT+AGG - 8583:27239-27258 MS.gene27786:intergenic 45.0%
CACAAATTGTCTCGCACGAA+GGG - 8583:28320-28339 MS.gene27787:intergenic 45.0%
CCCTCAACAACATTGCTTAC+TGG + 8583:28363-28382 MS.gene27787:CDS 45.0%
GCCAGTAAGCAATGTTGTTG+AGG - 8583:28367-28386 MS.gene27787:intergenic 45.0%
GTAGCCCAAGGAAGAAACTT+AGG + 8583:26699-26718 MS.gene27787:CDS 45.0%
GTGAGCCTAAGTTTCTTCCT+TGG - 8583:26707-26726 MS.gene27787:intergenic 45.0%
GTGTTGCGAATCTCTAACAG+AGG + 8583:28191-28210 MS.gene27787:CDS 45.0%
AGGCTAGACAAGTAGAGGTG+TGG + 8583:27542-27561 MS.gene27787:CDS 50.0%
CCACAAATTGTCTCGCACGA+AGG - 8583:28321-28340 MS.gene27787:intergenic 50.0%
CCTTCGTGCGAGACAATTTG+TGG + 8583:28318-28337 MS.gene27787:CDS 50.0%
GCACGGAGCAGTAAATTACC+TGG - 8583:27584-27603 MS.gene27787:intergenic 50.0%
GGTTTCAAAATCGGAGAGCC+AGG + 8583:27563-27582 MS.gene27787:CDS 50.0%
TCGTGCGAGACAATTTGTGG+TGG + 8583:28321-28340 MS.gene27787:CDS 50.0%
TCTGCAGGCTAGACAAGTAG+AGG + 8583:27537-27556 MS.gene27787:CDS 50.0%
! CAGATGGTAAGTGTTGCTGC+TGG - 8583:28297-28316 MS.gene27787:intergenic 50.0%
TGTCTCGCACGAAGGGCAGA+TGG - 8583:28313-28332 MS.gene27787:intergenic 60.0%


Chromosome Type Strat End Strand Name
8583 gene 26696 28419 26696 ID=MS.gene27787
8583 mRNA 26696 28419 26696 ID=MS.gene27787.t1;Parent=MS.gene27787
8583 exon 26696 26784 26696 ID=MS.gene27787.t1.exon1;Parent=MS.gene27787.t1
8583 CDS 26696 26784 26696 ID=cds.MS.gene27787.t1;Parent=MS.gene27787.t1
8583 exon 27486 27584 27486 ID=MS.gene27787.t1.exon2;Parent=MS.gene27787.t1
8583 CDS 27486 27584 27486 ID=cds.MS.gene27787.t1;Parent=MS.gene27787.t1
8583 exon 28146 28419 28146 ID=MS.gene27787.t1.exon3;Parent=MS.gene27787.t1
8583 CDS 28146 28419 28146 ID=cds.MS.gene27787.t1;Parent=MS.gene27787.t1
Gene Sequence

>MS.gene27787

ATGGTAGCCCAAGGAAGAAACTTAGGCTCACAAAACAACAATCAGAAGTTTTGGAGGAAAACTTCAAAGAGCATTCTACTCTTAATCCGGTACCTTAATTCATCAATATTCTCATATCTTTGGTTGCTTTAAATAATTCTTTTGCTCCTATACCACGTACTTTTGTTTTGATATTTTTATTTTAGTACTAATAACTATTTTATATATATAAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAGACACACACGCACAGTATTTTTCTCATGGCACACTAATTGTGTGTAGCATATAGTAAATGAAGAATATGATATCATTTTAAACGAGTTTTCTCGATGATATTTGATTTTATTAGAATTAGATCTTGGTCTTGGTTGATTGTGTTTGGTCTAAAACTGAGAAGACCTTAGATTGAGTTTGCTTCATAGTTTCAAAATTTATTCTCTTGGATTACATTTTCTAGGAATATTATTCCCACCAAAGTGTTTGGTAAAATCTTGATATTCCTAGGAAAGTTGGTAGGTGTGTTTTATTTTCCAGAATAAATAATTTCCTGAAACAATTTTTATAAACTTGTACGGAATATAATAATATTAAATTCTAACCTCATTTTTTTAGAAATGTTTCTGGACCTTGAAACAAACACACCTTAGAATTTAATTTTTTTTCTAATTTGTGCAATTGCTACATCCAATCCAATGTATTACAAATTTTGTAGCATGAGTATATGTTGCTTTGTTATTATTAATAAAAAGAAAATCTCATTTTCTTCAGAAGCAAAAGCAAGCATTAGCAAATAAGCTGAATCTGCAGGCTAGACAAGTAGAGGTGTGGTTTCAAAATCGGAGAGCCAGGTAATTTACTGCTCCGTGCGTTTTAAGATAGTATCGTTTAAAGTTCTTTTATATAGATTAAAAAATTTACAAAACTGTTTATCCATTAGCAATTGTCATGATGATGCATAACAAAATATAATGTCTTGAGACTTATGTACACACAAAAAGTTGTGATAAAATTGAAATAATGAAATTATACTATTGATGTTACATTAAAAAGTACCATATATTTTACTTCTTTGTTTTTTTTTACTGGATCGCACATACTTTACTTTATTGCTTCTTTAATAGATAAATGTTACTTTTTTTAATAATAAATAAATGTTACTTGTTAGTATATATATTGTTAGAGCGAAAGGAGAAAACTTAGATGGCACGGATCTCAACTCTCCATATTGCGCACGTAGAGAATACTCGATTTACACACACAAAAAATGTTAACAGCTAGGGTCAAACCCTAAACCTCCTTCTAAACTTTTTAAACGTCTCGACCCTTTGATCGCATACTTTACCTTATTCCTCAGATTTTGTGAAATTATATTTCTTGCATATACTAATGATTACTATATACATGTTCAGAACAAAGCTGAAACAAACGGAATCTGATTGTGAAATACTGAAGAAGTGTTGCGAATCTCTAACAGAGGAGAACAAGAAGTTGCAAAAGGAGCTACAAGAACTAAAGTCAATGCAAACAGTAGCAGCAGCACCCTTTTATATGAAGATTCCAGCAGCAACACTTACCATCTGCCCTTCGTGCGAGACAATTTGTGGTGGCAACAACAATGGCTCCTTACCCTCAACAACATTGCTTACTGGCTCAAAGAATCATCATTTCCATAAAAACTTTTTG

Protein sequence

>MS.gene27787.t1

MVAQGRNLGSQNNNQKFWRKTSKSILLLIRKSHFLQKQKQALANKLNLQARQVEVWFQNRRARTKLKQTESDCEILKKCCESLTEENKKLQKELQELKSMQTVAAAPFYMKIPAATLTICPSCETICGGNNNGSLPSTTLLTGSKNHHFHKNFL