Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27792.t1 | XP_013465939.1 | 96.5 | 114 | 4 | 0 | 1 | 114 | 655 | 768 | 4.80E-56 | 226.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27792.t1 | A0A072VEF6 | 96.5 | 114 | 4 | 0 | 1 | 114 | 655 | 768 | 3.5e-56 | 226.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051744 | MS.gene27792 | -0.800506 | 1.40E-48 | -1.69E-46 |
| MS.gene054576 | MS.gene27792 | -0.811725 | 6.06E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27792.t1 | MTR_1g017170 | 96.491 | 114 | 4 | 0 | 1 | 114 | 655 | 768 | 1.78e-77 | 243 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGAGCATTCAGATGGGCTT+GGG | 0.249984 | 1.3:-5347305 | None:intergenic |
| CCCCTTGTCCTCTGGTGGGC+TGG | 0.281593 | 1.3:-5347325 | None:intergenic |
| ATGCGGCATGGGTTGTTTGT+AGG | 0.325375 | 1.3:-5347280 | None:intergenic |
| CTGGAGCATTCAGATGGGCT+TGG | 0.325575 | 1.3:-5347306 | None:intergenic |
| CCAGAGGACAAGGGGATTGC+TGG | 0.342797 | 1.3:+5347333 | MS.gene27792:CDS |
| GGGCTTGGGGCATGCGGCAT+GGG | 0.367202 | 1.3:-5347291 | None:intergenic |
| TGGGCTTGGGGCATGCGGCA+TGG | 0.405225 | 1.3:-5347292 | None:intergenic |
| CCAGCAATCCCCTTGTCCTC+TGG | 0.422853 | 1.3:-5347333 | None:intergenic |
| TTAATTAGATGTTCATGCAC+TGG | 0.432990 | 1.3:-5347598 | None:intergenic |
| GGTGGGCTGGAGCATTCAGA+TGG | 0.436201 | 1.3:-5347312 | None:intergenic |
| GCAATCCCCTTGTCCTCTGG+TGG | 0.441289 | 1.3:-5347330 | None:intergenic |
| CTCCAGCCCACCAGAGGACA+AGG | 0.456637 | 1.3:+5347323 | MS.gene27792:CDS |
| TGCACTGGATCGGTCACATC+AGG | 0.458565 | 1.3:-5347583 | None:intergenic |
| TCGCACACTGAGTCCGACTT+TGG | 0.459007 | 1.3:+5347498 | MS.gene27792:CDS |
| AGATGAACAATGTGGAAAGA+TGG | 0.464525 | 1.3:+5347230 | MS.gene27792:CDS |
| GGAGCATTCAGATGGGCTTG+GGG | 0.465800 | 1.3:-5347304 | None:intergenic |
| GATTTATGAACAATTGCGTT+CGG | 0.468079 | 1.3:+5347257 | MS.gene27792:CDS |
| CATGAACATCTAATTAAAGA+TGG | 0.487890 | 1.3:+5347604 | MS.gene27792:CDS |
| GAGTCCGACTTTGGATATAT+CGG | 0.493012 | 1.3:+5347507 | MS.gene27792:CDS |
| CAATCCCCTTGTCCTCTGGT+GGG | 0.494251 | 1.3:-5347329 | None:intergenic |
| CAGAGGACAAGGGGATTGCT+GGG | 0.509600 | 1.3:+5347334 | MS.gene27792:CDS |
| TTGGATATATCGGTACTTGG+TGG | 0.513625 | 1.3:+5347517 | MS.gene27792:CDS |
| TCAGATGGGCTTGGGGCATG+CGG | 0.514701 | 1.3:-5347297 | None:intergenic |
| TTCTTATTCAATGTACAAGC+GGG | 0.517512 | 1.3:-5347556 | None:intergenic |
| GGACTCAGTGTGCGATGGAT+AGG | 0.519782 | 1.3:-5347490 | None:intergenic |
| ACTTCAGAAGATATCGAGAA+AGG | 0.520959 | 1.3:+5347357 | MS.gene27792:CDS |
| GTGGGCTGGAGCATTCAGAT+GGG | 0.523832 | 1.3:-5347311 | None:intergenic |
| ACTTTGGATATATCGGTACT+TGG | 0.524263 | 1.3:+5347514 | MS.gene27792:CDS |
| AGTGTGCGATGGATAGGTCC+TGG | 0.558406 | 1.3:-5347484 | None:intergenic |
| CCAGCCCACCAGAGGACAAG+GGG | 0.566661 | 1.3:+5347325 | MS.gene27792:CDS |
| AAGTCGGACTCAGTGTGCGA+TGG | 0.587730 | 1.3:-5347495 | None:intergenic |
| ATTCTTATTCAATGTACAAG+CGG | 0.590606 | 1.3:-5347557 | None:intergenic |
| TAGATGTTCATGCACTGGAT+CGG | 0.605985 | 1.3:-5347593 | None:intergenic |
| GTCATTAAACAGACTAGACC+AGG | 0.622117 | 1.3:+5347466 | MS.gene27792:CDS |
| TCCAGCCCACCAGAGGACAA+GGG | 0.640270 | 1.3:+5347324 | MS.gene27792:CDS |
| GATAAGAAAGATGAACAATG+TGG | 0.654302 | 1.3:+5347222 | MS.gene27792:CDS |
| TGAATGCTCCAGCCCACCAG+AGG | 0.667039 | 1.3:+5347317 | MS.gene27792:CDS |
| AGTACCGATATATCCAAAGT+CGG | 0.675445 | 1.3:-5347511 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTCTTATTCAATGTACAAG+CGG | - | chr1.3:5347560-5347579 | None:intergenic | 25.0% |
| ! | CATGAACATCTAATTAAAGA+TGG | + | chr1.3:5347604-5347623 | MS.gene27792:CDS | 25.0% |
| !! | AAGGTTTGTTTGCTTTATAA+TGG | + | chr1.3:5347376-5347395 | MS.gene27792:intron | 25.0% |
| !!! | ATGTTTGCTTTTCATTTTTC+AGG | + | chr1.3:5347439-5347458 | MS.gene27792:intron | 25.0% |
| GATAAGAAAGATGAACAATG+TGG | + | chr1.3:5347222-5347241 | MS.gene27792:CDS | 30.0% | |
| GATTTATGAACAATTGCGTT+CGG | + | chr1.3:5347257-5347276 | MS.gene27792:CDS | 30.0% | |
| TGTTTGCTTTATAATGGTTG+AGG | + | chr1.3:5347382-5347401 | MS.gene27792:intron | 30.0% | |
| TTAATTAGATGTTCATGCAC+TGG | - | chr1.3:5347601-5347620 | None:intergenic | 30.0% | |
| TTCTTATTCAATGTACAAGC+GGG | - | chr1.3:5347559-5347578 | None:intergenic | 30.0% | |
| ACTTCAGAAGATATCGAGAA+AGG | + | chr1.3:5347357-5347376 | MS.gene27792:CDS | 35.0% | |
| ACTTTGGATATATCGGTACT+TGG | + | chr1.3:5347514-5347533 | MS.gene27792:CDS | 35.0% | |
| AGATGAACAATGTGGAAAGA+TGG | + | chr1.3:5347230-5347249 | MS.gene27792:CDS | 35.0% | |
| TCTGGGCAATGTTTATAACT+TGG | + | chr1.3:5347414-5347433 | MS.gene27792:intron | 35.0% | |
| ! | GGTTGAGGTTTCAATTTTCT+GGG | + | chr1.3:5347397-5347416 | MS.gene27792:intron | 35.0% |
| ! | TGGTTGAGGTTTCAATTTTC+TGG | + | chr1.3:5347396-5347415 | MS.gene27792:intron | 35.0% |
| !! | AGTACCGATATATCCAAAGT+CGG | - | chr1.3:5347514-5347533 | None:intergenic | 35.0% |
| GAGTCCGACTTTGGATATAT+CGG | + | chr1.3:5347507-5347526 | MS.gene27792:CDS | 40.0% | |
| GTCATTAAACAGACTAGACC+AGG | + | chr1.3:5347466-5347485 | MS.gene27792:CDS | 40.0% | |
| TAGATGTTCATGCACTGGAT+CGG | - | chr1.3:5347596-5347615 | None:intergenic | 40.0% | |
| TTGGATATATCGGTACTTGG+TGG | + | chr1.3:5347517-5347536 | MS.gene27792:CDS | 40.0% | |
| ! | ATGCGGCATGGGTTGTTTGT+AGG | - | chr1.3:5347283-5347302 | None:intergenic | 50.0% |
| ! | TGGAGCATTCAGATGGGCTT+GGG | - | chr1.3:5347308-5347327 | None:intergenic | 50.0% |
| AAGTCGGACTCAGTGTGCGA+TGG | - | chr1.3:5347498-5347517 | None:intergenic | 55.0% | |
| AGTGTGCGATGGATAGGTCC+TGG | - | chr1.3:5347487-5347506 | None:intergenic | 55.0% | |
| CAATCCCCTTGTCCTCTGGT+GGG | - | chr1.3:5347332-5347351 | None:intergenic | 55.0% | |
| CAGAGGACAAGGGGATTGCT+GGG | + | chr1.3:5347334-5347353 | MS.gene27792:CDS | 55.0% | |
| GGACTCAGTGTGCGATGGAT+AGG | - | chr1.3:5347493-5347512 | None:intergenic | 55.0% | |
| TCGCACACTGAGTCCGACTT+TGG | + | chr1.3:5347498-5347517 | MS.gene27792:CDS | 55.0% | |
| TGCACTGGATCGGTCACATC+AGG | - | chr1.3:5347586-5347605 | None:intergenic | 55.0% | |
| ! | CTGGAGCATTCAGATGGGCT+TGG | - | chr1.3:5347309-5347328 | None:intergenic | 55.0% |
| ! | GGAGCATTCAGATGGGCTTG+GGG | - | chr1.3:5347307-5347326 | None:intergenic | 55.0% |
| !! | GTGGGCTGGAGCATTCAGAT+GGG | - | chr1.3:5347314-5347333 | None:intergenic | 55.0% |
| CCAGAGGACAAGGGGATTGC+TGG | + | chr1.3:5347333-5347352 | MS.gene27792:CDS | 60.0% | |
| CCAGCAATCCCCTTGTCCTC+TGG | - | chr1.3:5347336-5347355 | None:intergenic | 60.0% | |
| GCAATCCCCTTGTCCTCTGG+TGG | - | chr1.3:5347333-5347352 | None:intergenic | 60.0% | |
| TCCAGCCCACCAGAGGACAA+GGG | + | chr1.3:5347324-5347343 | MS.gene27792:CDS | 60.0% | |
| TGAATGCTCCAGCCCACCAG+AGG | + | chr1.3:5347317-5347336 | MS.gene27792:CDS | 60.0% | |
| ! | TCAGATGGGCTTGGGGCATG+CGG | - | chr1.3:5347300-5347319 | None:intergenic | 60.0% |
| !! | GGTGGGCTGGAGCATTCAGA+TGG | - | chr1.3:5347315-5347334 | None:intergenic | 60.0% |
| CCAGCCCACCAGAGGACAAG+GGG | + | chr1.3:5347325-5347344 | MS.gene27792:CDS | 65.0% | |
| CTCCAGCCCACCAGAGGACA+AGG | + | chr1.3:5347323-5347342 | MS.gene27792:CDS | 65.0% | |
| GGGCTTGGGGCATGCGGCAT+GGG | - | chr1.3:5347294-5347313 | None:intergenic | 70.0% | |
| TGGGCTTGGGGCATGCGGCA+TGG | - | chr1.3:5347295-5347314 | None:intergenic | 70.0% | |
| ! | CCCCTTGTCCTCTGGTGGGC+TGG | - | chr1.3:5347328-5347347 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 5347207 | 5347633 | 5347207 | ID=MS.gene27792 |
| chr1.3 | mRNA | 5347207 | 5347633 | 5347207 | ID=MS.gene27792.t1;Parent=MS.gene27792 |
| chr1.3 | exon | 5347207 | 5347378 | 5347207 | ID=MS.gene27792.t1.exon1;Parent=MS.gene27792.t1 |
| chr1.3 | CDS | 5347207 | 5347378 | 5347207 | ID=cds.MS.gene27792.t1;Parent=MS.gene27792.t1 |
| chr1.3 | exon | 5347461 | 5347633 | 5347461 | ID=MS.gene27792.t1.exon2;Parent=MS.gene27792.t1 |
| chr1.3 | CDS | 5347461 | 5347633 | 5347461 | ID=cds.MS.gene27792.t1;Parent=MS.gene27792.t1 |
| Gene Sequence |
| Protein sequence |
