AlfalfaGEDB | MS.gene27850

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27850.t1	RHN42691.1	94.1	136	6	1	1	136	189	322	5.40E-62	246.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27850.t1	G7L7A4	94.1	136	6	1	1	136	71	204	3.9e-62	246.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049151	MS.gene27850	0.815904	7.28E-52	-1.69E-46
MS.gene05119	MS.gene27850	-0.812115	4.99E-51	-1.69E-46
MS.gene053836	MS.gene27850	0.801604	8.32E-49	-1.69E-46
MS.gene054185	MS.gene27850	0.80368	3.11E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Find 21 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGCAATCCGGGAACAGTTT+GGG	0.217269	4.1:+13148683	MS.gene27850:CDS
GACCAAGGAGAAAATATAAT+TGG	0.266280	4.1:+13148773	MS.gene27850:CDS
ACAAAGATTCAAACACAAAT+TGG	0.268354	4.1:+13148914	MS.gene27850:CDS
GTTTGAATCTTTGTAATATC+TGG	0.300279	4.1:-13148905	None:intergenic
CAGTTGCTGTGACAATCTTT+TGG	0.304172	4.1:-13148871	None:intergenic
TCGGCAATCCGGGAACAGTT+TGG	0.321768	4.1:+13148682	MS.gene27850:CDS
TATTCCCAGCGTGGATGATA+AGG	0.422594	4.1:+13148715	MS.gene27850:CDS
ACACAAATTGGTGATGCAAT+AGG	0.433183	4.1:+13148926	MS.gene27850:CDS
GAGAGATTGGTTCGGCAATC+CGG	0.440927	4.1:+13148671	None:intergenic
GGCAATCCGGGAACAGTTTG+GGG	0.442842	4.1:+13148684	MS.gene27850:CDS
AGAGATTGGTTCGGCAATCC+GGG	0.458252	4.1:+13148672	None:intergenic
AAAATATAATTGGATTATGC+GGG	0.519176	4.1:+13148783	MS.gene27850:CDS
GAAAATATAATTGGATTATG+CGG	0.532748	4.1:+13148782	MS.gene27850:CDS
TGTCGTTTGAGAGATTGGTT+CGG	0.553620	4.1:+13148663	None:intergenic
ATGCAACAAGTTGATTCAGC+CGG	0.577268	4.1:-13148974	None:intergenic
TTAACCTTATCATCCACGCT+GGG	0.579493	4.1:-13148719	None:intergenic
GCAATCCGGGAACAGTTTGG+GGG	0.584978	4.1:+13148685	MS.gene27850:CDS
GCACTAAGAGTGAGAGACCA+AGG	0.594788	4.1:+13148758	MS.gene27850:CDS
TTTAACCTTATCATCCACGC+TGG	0.639042	4.1:-13148720	None:intergenic
GGGCATTTATATTCCCAGCG+TGG	0.677027	4.1:+13148706	MS.gene27850:CDS
CAATCCGGGAACAGTTTGGG+GGG	0.685818	4.1:+13148686	MS.gene27850:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATATAATTGGATTATGC+GGG	+	chr4.1:13148783-13148802	MS.gene27850:CDS	20.0%
!!	GAAAATATAATTGGATTATG+CGG	+	chr4.1:13148782-13148801	MS.gene27850:CDS	20.0%
!!!	TCTGAATTTTGATGATAAAA+CGG	+	chr4.1:13148949-13148968	MS.gene27850:CDS	20.0%
!!!	TTTTGGTAATAGAAAAAGAA+TGG	+	chr4.1:13149000-13149019	MS.gene27850:CDS	20.0%
!	ACAAAGATTCAAACACAAAT+TGG	+	chr4.1:13148914-13148933	MS.gene27850:CDS	25.0%
!	GTTTGAATCTTTGTAATATC+TGG	-	chr4.1:13148908-13148927	None:intergenic	25.0%
!!	ATCCAATTATATTTTCTCCT+TGG	-	chr4.1:13148778-13148797	None:intergenic	25.0%
	GACCAAGGAGAAAATATAAT+TGG	+	chr4.1:13148773-13148792	MS.gene27850:CDS	30.0%
!!	TTTTGATGATAAAACGGATC+CGG	+	chr4.1:13148955-13148974	MS.gene27850:CDS	30.0%
!!!	TCAACTTGTTGCATGTTTTT+TGG	+	chr4.1:13148983-13149002	MS.gene27850:CDS	30.0%
!!	ACACAAATTGGTGATGCAAT+AGG	+	chr4.1:13148926-13148945	MS.gene27850:CDS	35.0%
!!!	GAATGCTTGACTCTTTTTTC+TGG	-	chr4.1:13148809-13148828	None:intergenic	35.0%
	TTAACCTTATCATCCACGCT+GGG	-	chr4.1:13148722-13148741	None:intergenic	40.0%
	TTTAACCTTATCATCCACGC+TGG	-	chr4.1:13148723-13148742	None:intergenic	40.0%
!	CAGTTGCTGTGACAATCTTT+TGG	-	chr4.1:13148874-13148893	None:intergenic	40.0%
!!	ATGCAACAAGTTGATTCAGC+CGG	-	chr4.1:13148977-13148996	None:intergenic	40.0%
	TATTCCCAGCGTGGATGATA+AGG	+	chr4.1:13148715-13148734	MS.gene27850:CDS	45.0%
	GCACTAAGAGTGAGAGACCA+AGG	+	chr4.1:13148758-13148777	MS.gene27850:CDS	50.0%
	GGGCATTTATATTCCCAGCG+TGG	+	chr4.1:13148706-13148725	MS.gene27850:CDS	50.0%
	AATGCCCCCCAAACTGTTCC+CGG	-	chr4.1:13148693-13148712	None:intergenic	55.0%
	CAATCCGGGAACAGTTTGGG+GGG	+	chr4.1:13148686-13148705	MS.gene27850:CDS	55.0%
	CGGCAATCCGGGAACAGTTT+GGG	+	chr4.1:13148683-13148702	MS.gene27850:CDS	55.0%
	GCAATCCGGGAACAGTTTGG+GGG	+	chr4.1:13148685-13148704	MS.gene27850:CDS	55.0%
	GGCAATCCGGGAACAGTTTG+GGG	+	chr4.1:13148684-13148703	MS.gene27850:CDS	55.0%
	TCGGCAATCCGGGAACAGTT+TGG	+	chr4.1:13148682-13148701	MS.gene27850:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	13148676	13149084	13148676	ID=MS.gene27850
chr4.1	mRNA	13148676	13149084	13148676	ID=MS.gene27850.t1;Parent=MS.gene27850
chr4.1	exon	13148676	13149084	13148676	ID=MS.gene27850.t1.exon1;Parent=MS.gene27850.t1
chr4.1	CDS	13148676	13149084	13148676	ID=cds.MS.gene27850.t1;Parent=MS.gene27850.t1

Gene Sequence

>MS.gene27850

ATTGGTTCGGCAATCCGGGAACAGTTTGGGGGGCATTTATATTCCCAGCGTGGATGATAAGGTTAAAGAACAAATTATGAGTGCACTAAGAGTGAGAGACCAAGGAGAAAATATAATTGGATTATGCGGGCCAGAAAAAAGAGTCAAGCATTCTGCAAAAACAGCTATAAGAAGAGCTGAGAGAGATCAATTGTTCCAAAAGATTGTCACAGCAACTGTGACAAAGAAGCCAGATATTACAAAGATTCAAACACAAATTGGTGATGCAATAGGTCTGAATTTTGATGATAAAACGGATCCGGCTGAATCAACTTGTTGCATGTTTTTTGGTAATAGAAAAAGAATGGCAACTGCAGAAAGAGCTCATCTTTTGTGTGCTAAGATGAAAGAGCTGCAAACAGTCCTAGTT

Protein sequence

>MS.gene27850.t1

IGSAIREQFGGHLYSQRGUUGURTNYECTKSERPRRKYNWIMRARKKSQAFCKNSYKKSUERSIVPKDCHSNCDKEARYYKDSNTNWUCNRSEFUUUNGSGUINLLHVFWUUKKNGNCRKSSSFVCUDERAANSPS

M. sativa cultivar XinJiangDaYe / MS.gene27850