AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27859

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27859.t1 MTR_8g087770 85.373 670 92 3 1 669 1 665 0.0 1152
MS.gene27859.t1 MTR_8g087750 82.406 665 112 3 6 669 11 671 0.0 1121
MS.gene27859.t1 MTR_8g087763 81.858 226 40 1 373 598 4 228 3.46e-131 391
MS.gene27859.t1 MTR_4g093750 33.666 401 241 10 66 451 35 425 8.08e-44 168
MS.gene27859.t1 MTR_4g093750 39.373 287 154 7 66 338 35 315 6.23e-43 166
MS.gene27859.t1 MTR_2g009690 38.246 285 164 6 62 336 40 322 3.56e-40 158
MS.gene27859.t1 MTR_5g009150 28.726 463 304 12 68 517 35 484 1.80e-34 139
MS.gene27859.t1 MTR_1g032550 34.062 320 179 10 68 368 35 341 3.08e-34 139
MS.gene27859.t1 MTR_8g105580 33.758 314 187 7 68 368 35 340 7.47e-34 137
MS.gene27859.t1 MTR_8g105580 33.758 314 187 7 68 368 35 340 9.93e-34 137
MS.gene27859.t1 MTR_8g009510 35.202 321 174 11 68 368 40 346 2.36e-33 136
MS.gene27859.t1 MTR_7g451010 34.688 320 177 10 68 368 40 346 1.52e-32 134
MS.gene27859.t1 MTR_4g098920 34.169 319 180 9 68 368 34 340 2.33e-31 130
MS.gene27859.t1 MTR_8g099550 32.803 314 191 7 68 368 34 340 5.08e-31 129
MS.gene27859.t1 MTR_8g027675 41.176 187 105 3 68 251 34 218 7.56e-31 128
MS.gene27859.t1 MTR_4g085860 73.684 76 19 1 205 279 2 77 6.33e-30 113
MS.gene27859.t1 MTR_6g045107 72.368 76 20 1 205 279 2 77 5.73e-28 107
MS.gene27859.t1 MTR_8g009510 31.474 251 142 8 135 368 2 239 1.99e-18 89.7
MS.gene27859.t1 MTR_8g463270 29.907 214 139 4 64 268 42 253 1.03e-16 84.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27859.t1 AT1G60500 59.027 637 258 2 34 669 31 665 0.0 784
MS.gene27859.t1 AT1G60530 71.379 290 75 1 17 306 19 300 1.58e-142 419
MS.gene27859.t1 AT2G14120 29.505 444 290 11 24 451 1 437 7.21e-39 154
MS.gene27859.t1 AT2G14120 33.234 337 207 7 24 345 1 334 8.07e-39 154
MS.gene27859.t1 AT2G14120 29.505 444 290 11 24 451 1 437 8.88e-39 154
MS.gene27859.t1 AT2G14120 29.505 444 290 11 24 451 1 437 1.01e-38 154
MS.gene27859.t1 AT4G33650 29.705 441 268 11 35 451 30 452 2.08e-38 153
MS.gene27859.t1 AT4G33650 29.705 441 268 11 35 451 30 452 2.33e-38 152
MS.gene27859.t1 AT1G14830 33.439 314 189 7 68 368 35 341 7.22e-35 140
MS.gene27859.t1 AT5G42080 29.806 463 299 11 68 517 34 483 1.53e-34 140
MS.gene27859.t1 AT5G42080 29.806 463 299 11 68 517 34 483 1.53e-34 140
MS.gene27859.t1 AT3G60190 34.177 316 184 9 68 368 40 346 1.74e-33 137
MS.gene27859.t1 AT2G44590 24.682 628 398 23 68 671 35 611 7.40e-33 134
MS.gene27859.t1 AT5G42080 29.501 461 297 11 68 517 34 477 1.80e-32 133
MS.gene27859.t1 AT5G42080 33.548 310 194 6 68 368 34 340 1.91e-32 130
MS.gene27859.t1 AT3G61760 33.228 316 187 9 68 368 34 340 2.45e-30 125
MS.gene27859.t1 AT3G61760 33.228 316 187 9 68 368 34 340 2.88e-30 126
MS.gene27859.t1 AT3G61760 33.228 316 187 9 68 368 34 340 3.07e-30 127
MS.gene27859.t1 AT2G44590 23.567 628 388 24 68 671 35 594 1.22e-23 106
MS.gene27859.t1 AT2G44590 29.641 334 186 12 68 385 35 335 5.53e-23 104
MS.gene27859.t1 AT1G53140 27.848 237 157 6 43 268 36 269 1.09e-14 78.6

Find 131 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAATAGATTTATCTATTT+TGG 0.051549 4.1:-13004588 None:intergenic
TTAATCTTCTTAATGATTTC+AGG 0.171207 4.1:-13004674 None:intergenic
GGAAAATTTGCCTGCCAATT+TGG 0.204709 4.1:+13004727 MS.gene27859:CDS
CTTGAGGAAGCCAAGATTAT+TGG 0.224459 4.1:+13004974 MS.gene27859:CDS
TTCTTAACAATCAAAGTTAA+AGG 0.234341 4.1:-13004167 None:intergenic
GCTGTTCTTGGCATGAAATT+TGG 0.254803 4.1:-13005001 None:intergenic
GAACTTTGATTTCGTCCATT+AGG 0.265918 4.1:-13004952 None:intergenic
GGGAAGTTGGATTCCCTCTT+TGG 0.273448 4.1:-13003883 None:intergenic
TGACAGTGTTTGGAACTATT+TGG 0.274905 4.1:+13005084 MS.gene27859:CDS
GAGGAGTCTCCTTCAATATC+AGG 0.303692 4.1:+13005613 MS.gene27859:CDS
TTACTACTTGTGAGTCCATT+AGG 0.307685 4.1:+13004354 MS.gene27859:CDS
GCCTGCCAATTTGGCATCAT+TGG 0.308754 4.1:+13004736 MS.gene27859:CDS
CAAAGCACCAGAAGGCTTGC+TGG 0.312662 4.1:+13004436 MS.gene27859:CDS
ATTTGATCATAGATATTATC+AGG 0.327362 4.1:-13004257 None:intergenic
TATTGTAGGAATTCCTGTTT+TGG 0.327596 4.1:+13004604 MS.gene27859:CDS
TTCAAGATAATACTTTCTTC+TGG 0.339329 4.1:-13004308 None:intergenic
ATCAGCCAATGATGCCAAAT+TGG 0.340224 4.1:-13004741 None:intergenic
AATGTAAGAAAAGCTGTTCT+TGG 0.343865 4.1:-13005013 None:intergenic
TAATATCTATGATCAAATTA+AGG 0.347385 4.1:+13004262 MS.gene27859:CDS
TTTGTGTAAGGAATAGAATT+GGG 0.352743 4.1:+13004501 MS.gene27859:CDS
TCTTAACAATCAAAGTTAAA+GGG 0.360721 4.1:-13004166 None:intergenic
AATGATTTCAGGCAAAGTTT+TGG 0.361226 4.1:-13004663 None:intergenic
TTAACCATGGTTGATCTTCC+AGG 0.367637 4.1:+13004206 MS.gene27859:CDS
CTTCCCACCATTGTTGTTGT+TGG 0.371021 4.1:+13003900 MS.gene27859:CDS
ACACTGTCAACAAAATTAAC+TGG 0.371215 4.1:-13005070 None:intergenic
TCACAAACTGTTGATAAAAC+TGG 0.375821 4.1:+13004380 MS.gene27859:CDS
GTTTGTGTAAGGAATAGAAT+TGG 0.375877 4.1:+13004500 MS.gene27859:CDS
AAAATAGATAAATCTATTGT+AGG 0.376484 4.1:+13004590 MS.gene27859:CDS
GACTCAGGCCTTTGACTTGA+AGG 0.382103 4.1:+13005429 MS.gene27859:CDS
ATTTGGAAGACCAATAATCT+TGG 0.383624 4.1:-13004984 None:intergenic
AGATCAACCATGGTTAAATC+TGG 0.385650 4.1:-13004200 None:intergenic
GGCTAATAGTTTACATGAGT+TGG 0.395161 4.1:+13004706 MS.gene27859:CDS
TCATTGTAAGATGATACTAT+TGG 0.397762 4.1:-13003807 None:intergenic
GTCTTGGTTATGTTTGTGTA+AGG 0.401697 4.1:+13004489 MS.gene27859:CDS
TGTCTCTTCCCTGATATTGA+AGG 0.410432 4.1:-13005622 None:intergenic
CATTACTTCAGAGGAATGTA+AGG 0.420635 4.1:+13005032 MS.gene27859:CDS
CAAGAGTACCATTGGTTATG+AGG 0.425077 4.1:+13004000 MS.gene27859:CDS
AACAGTTTGTGACATCCTAA+TGG 0.425745 4.1:-13004369 None:intergenic
ACAAGCTTCTGTGCCAAAAC+AGG 0.426561 4.1:-13004617 None:intergenic
ACAGCCACTGAGGAGCTTGC+TGG 0.430442 4.1:+13004119 MS.gene27859:CDS
TCAAGTACTGTGAATCTTGA+AGG 0.433218 4.1:+13005355 MS.gene27859:CDS
CAATGCATTTGCTTATCATC+AGG 0.438737 4.1:-13004848 None:intergenic
TAAGAAGATTAATGAGAAGT+TGG 0.440046 4.1:+13004685 MS.gene27859:CDS
CCTCAGCACAATCTTCCAAT+AGG 0.443004 4.1:-13005464 None:intergenic
GCATTGCACCGCTAGATTAG+TGG 0.443244 4.1:+13004865 MS.gene27859:CDS
ACCAGAAGGCTTGCTGGAAA+AGG 0.448222 4.1:+13004442 MS.gene27859:CDS
AGGCAAGGCTAATTGCCTAT+TGG 0.448827 4.1:+13005449 MS.gene27859:CDS
ATGTAACCCTGAGTACTTGC+AGG 0.450401 4.1:+13005267 MS.gene27859:CDS
ACAAGCTCTGGCTGTGGAAG+TGG 0.452099 4.1:-13004035 None:intergenic
TTATACTCCTGCAAGTACTC+AGG 0.459259 4.1:-13005274 None:intergenic
GGAAGAGACAGAAGTTGAGC+AGG 0.460748 4.1:+13005635 MS.gene27859:CDS
AGGGGTGTTGGAAATCCCTT+TGG 0.462637 4.1:-13004147 None:intergenic
CAACAACAATGGTGGGAAGT+TGG 0.467309 4.1:-13003896 None:intergenic
GATGATGTTAACATCGGTCT+TGG 0.467639 4.1:+13004473 MS.gene27859:CDS
AACAAAACAGACATGACAAA+AGG 0.469116 4.1:-13005742 None:intergenic
TTCGTCCATTAGGAAATCCT+TGG 0.470036 4.1:-13004942 None:intergenic
TTATCAGGTTGGCCATGTAC+AGG 0.471735 4.1:-13004242 None:intergenic
GAGCTTGCTGGCACTGCCAA+AGG 0.478600 4.1:+13004131 MS.gene27859:CDS
GTACTCTTGTGCAAATTCCT+TGG 0.481903 4.1:-13003986 None:intergenic
GCCAATGATGCCAAATTGGC+AGG 0.486397 4.1:-13004737 None:intergenic
TATGACCAAGGATTTCCTAA+TGG 0.487515 4.1:+13004937 MS.gene27859:CDS
TTATACTCTAAAACAAGCTC+TGG 0.488227 4.1:-13004047 None:intergenic
AATCAAAGTTAAAGGGGTGT+TGG 0.500893 4.1:-13004159 None:intergenic
TGTGCTGCAAGGTGTTGAAA+TGG 0.500919 4.1:+13005225 MS.gene27859:CDS
TGCAAACTATTATCAGCTGC+AGG 0.502339 4.1:+13005141 MS.gene27859:CDS
CTTGAATGTTCTTTCTGCCA+CGG 0.502379 4.1:+13004325 MS.gene27859:CDS
TCAGCATTTGTTGTTGACTC+AGG 0.505197 4.1:+13005414 MS.gene27859:CDS
ATTACTCGTGTCCCTGTACA+TGG 0.509421 4.1:+13004230 MS.gene27859:CDS
GTTGTTGGTGATCAATCGTC+TGG 0.509934 4.1:+13003915 MS.gene27859:CDS
GATCATAGATATTATCAGGT+TGG 0.512244 4.1:-13004253 None:intergenic
AATTTGCACAAGAGTACCAT+TGG 0.513388 4.1:+13003992 MS.gene27859:CDS
AGTGTCCTTGAGTCATTAGC+TGG 0.515596 4.1:+13003945 MS.gene27859:CDS
TCAAATTAAGGATATTATCA+TGG 0.518955 4.1:+13004274 MS.gene27859:CDS
ATATAATCATAGCTTGCACT+TGG 0.531392 4.1:-13004640 None:intergenic
AATGAACTGTTATCAGCAAA+TGG 0.537861 4.1:+13005568 MS.gene27859:CDS
ATAAGTTTGCCACGTGGCCA+AGG 0.541062 4.1:+13003969 MS.gene27859:CDS
TGAAGAATCATCACTTGCAT+TGG 0.541598 4.1:+13003740 MS.gene27859:CDS
AGCTTGCTGGCACTGCCAAA+GGG 0.547647 4.1:+13004132 MS.gene27859:CDS
CTTAACAATCAAAGTTAAAG+GGG 0.548616 4.1:-13004165 None:intergenic
TCTAAAACAAGCTCTGGCTG+TGG 0.552834 4.1:-13004041 None:intergenic
GGACGAAATCAAAGTTCTTG+AGG 0.554038 4.1:+13004958 MS.gene27859:CDS
AACTAAAACAAACATAATGT+TGG 0.557553 4.1:-13005695 None:intergenic
TAGCCTTGCCTTCAAGTCAA+AGG 0.560724 4.1:-13005437 None:intergenic
TGGCAATGTTGATACGCCGT+AGG 0.566250 4.1:-13003863 None:intergenic
ACAGGGACACGAGTAATGCC+TGG 0.569922 4.1:-13004224 None:intergenic
ATCACCAACAACAACAATGG+TGG 0.572674 4.1:-13003904 None:intergenic
AACAAACATAATGTTGGCCA+CGG 0.573159 4.1:-13005689 None:intergenic
CGTATCAACATTGCCAAAGA+GGG 0.575382 4.1:+13003870 MS.gene27859:CDS
AGTGCCAGCAAGCTCCTCAG+TGG 0.578957 4.1:-13004123 None:intergenic
TATACTCCTGCAAGTACTCA+GGG 0.580347 4.1:-13005273 None:intergenic
TGTGAATGTTGATCTGCAAA+AGG 0.585319 4.1:+13005537 MS.gene27859:CDS
AGGAGTCTCCTTCAATATCA+GGG 0.586223 4.1:+13005614 MS.gene27859:CDS
AGCTAATGACTCAAGGACAC+TGG 0.593764 4.1:-13003943 None:intergenic
ATTGGAAGATTGTGCTGAGG+AGG 0.597029 4.1:+13005467 MS.gene27859:CDS
ATTACTTCAGAGGAATGTAA+GGG 0.598482 4.1:+13005033 MS.gene27859:CDS
TTATACCAGCTAATGACTCA+AGG 0.606048 4.1:-13003950 None:intergenic
TATCAGGTTGGCCATGTACA+GGG 0.611782 4.1:-13004241 None:intergenic
CAAGCAAGTTTCAACTGATG+AGG 0.612510 4.1:+13004073 MS.gene27859:CDS
GCGTATCAACATTGCCAAAG+AGG 0.613041 4.1:+13003869 MS.gene27859:CDS
TCTATTCAACATGTGCTGCA+AGG 0.615822 4.1:+13005214 MS.gene27859:CDS
TAGATGCCCTAGAAAACCTA+CGG 0.617872 4.1:+13003847 MS.gene27859:CDS
TGGTGTTGAGAGGCTGCTTG+AGG 0.618613 4.1:+13005594 MS.gene27859:CDS
TCACCAACAACAACAATGGT+GGG 0.623546 4.1:-13003903 None:intergenic
AGGCCTTTGACTTGAAGGCA+AGG 0.625900 4.1:+13005434 MS.gene27859:CDS
CTGTTATCAGCAAATGGTGG+TGG 0.626367 4.1:+13005574 MS.gene27859:CDS
GTGCAAATTCCTTGGCCACG+TGG 0.626948 4.1:-13003978 None:intergenic
TGGTATTCCAGATTTAACCA+TGG 0.634354 4.1:+13004193 MS.gene27859:CDS
TTGATCACCAACAACAACAA+TGG 0.636905 4.1:-13003907 None:intergenic
CAAATGGTGGTGGTGTTGAG+AGG 0.637671 4.1:+13005584 MS.gene27859:CDS
TTGTGTAAGGAATAGAATTG+GGG 0.639370 4.1:+13004502 MS.gene27859:CDS
GCTCTGGCTGTGGAAGTGGA+TGG 0.648549 4.1:-13004031 None:intergenic
GGTGCAACAACAAGTGGTTG+AGG 0.648777 4.1:-13003786 None:intergenic
AAAGCTGACAAAGCACCAGA+AGG 0.649435 4.1:+13004428 MS.gene27859:CDS
AATTTGCAGAAAAGCAGACA+TGG 0.650037 4.1:-13004765 None:intergenic
TGCTGAAAGTAATATGACCA+AGG 0.653435 4.1:+13004925 MS.gene27859:CDS
GAGAGAAAGCAAAGAGACCG+TGG 0.654345 4.1:+13005672 MS.gene27859:CDS
CAACATATCCACTAATCTAG+CGG 0.654700 4.1:-13004873 None:intergenic
ACTATTGGTGCAACAACAAG+TGG 0.655043 4.1:-13003792 None:intergenic
TGCTATAAACACAGCCACTG+AGG 0.658388 4.1:+13004109 MS.gene27859:CDS
ACCGCTGATGATGTTAACAT+CGG 0.662734 4.1:+13004467 MS.gene27859:CDS
ACCGATGTTAACATCATCAG+CGG 0.666783 4.1:-13004468 None:intergenic
CTGTTGATAAAACTGGTCTG+AGG 0.690962 4.1:+13004387 MS.gene27859:CDS
TTACTTCAGAGGAATGTAAG+GGG 0.701485 4.1:+13005034 MS.gene27859:CDS
AATGCCTGGAAGATCAACCA+TGG 0.705380 4.1:-13004210 None:intergenic
CCTATTGGAAGATTGTGCTG+AGG 0.716472 4.1:+13005464 MS.gene27859:CDS
GTTGATCATCAACATCAACA+TGG 0.719635 4.1:-13003764 None:intergenic
ACAAGTAGTAAAATCAACCG+TGG 0.733510 4.1:-13004342 None:intergenic
GCTGATAATAGTTTGCAGAG+TGG 0.741343 4.1:-13005135 None:intergenic
GAACTGTTATCAGCAAATGG+TGG 0.747464 4.1:+13005571 MS.gene27859:CDS
GCTGGTATAAGTTTGCCACG+TGG 0.749894 4.1:+13003963 MS.gene27859:CDS
ACTGTGAATCTTGAAGGACA+TGG 0.765829 4.1:+13005361 MS.gene27859:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAGATAAATCTATTGT+AGG + chr4.1:13004590-13004609 MS.gene27859:CDS 15.0%
!! TAATATCTATGATCAAATTA+AGG + chr4.1:13004262-13004281 MS.gene27859:CDS 15.0%
!!! TACAATAGATTTATCTATTT+TGG - chr4.1:13004591-13004610 None:intergenic 15.0%
!! AACTAAAACAAACATAATGT+TGG - chr4.1:13005698-13005717 None:intergenic 20.0%
!! ACTTTGATTGTTAAGAAAAA+TGG + chr4.1:13004173-13004192 MS.gene27859:CDS 20.0%
!! ATTTGATCATAGATATTATC+AGG - chr4.1:13004260-13004279 None:intergenic 20.0%
!! TCAAATTAAGGATATTATCA+TGG + chr4.1:13004274-13004293 MS.gene27859:CDS 20.0%
!! TCTTAACAATCAAAGTTAAA+GGG - chr4.1:13004169-13004188 None:intergenic 20.0%
!! TTAAAAAGATTCTTCTGATA+GGG + chr4.1:13004810-13004829 MS.gene27859:CDS 20.0%
!! TTAATCTTCTTAATGATTTC+AGG - chr4.1:13004677-13004696 None:intergenic 20.0%
!! TTCTTAACAATCAAAGTTAA+AGG - chr4.1:13004170-13004189 None:intergenic 20.0%
!!! AATAGAATTCTTTTTCTTCT+TGG - chr4.1:13005200-13005219 None:intergenic 20.0%
! CTTAAAAAGATTCTTCTGAT+AGG + chr4.1:13004809-13004828 MS.gene27859:CDS 25.0%
! CTTAACAATCAAAGTTAAAG+GGG - chr4.1:13004168-13004187 None:intergenic 25.0%
! TAAAAAGATTCTTCTGATAG+GGG + chr4.1:13004811-13004830 MS.gene27859:CDS 25.0%
! TAAGAAGATTAATGAGAAGT+TGG + chr4.1:13004685-13004704 MS.gene27859:CDS 25.0%
! TCATTGTAAGATGATACTAT+TGG - chr4.1:13003810-13003829 None:intergenic 25.0%
! TTCAAGATAATACTTTCTTC+TGG - chr4.1:13004311-13004330 None:intergenic 25.0%
! TTTGTGTAAGGAATAGAATT+GGG + chr4.1:13004501-13004520 MS.gene27859:CDS 25.0%
!! TTAATTTTGTTGACAGTGTT+TGG + chr4.1:13005074-13005093 MS.gene27859:CDS 25.0%
AACAAAACAGACATGACAAA+AGG - chr4.1:13005745-13005764 None:intergenic 30.0%
AATGAACTGTTATCAGCAAA+TGG + chr4.1:13005568-13005587 MS.gene27859:CDS 30.0%
AATGTAAGAAAAGCTGTTCT+TGG - chr4.1:13005016-13005035 None:intergenic 30.0%
ACACTGTCAACAAAATTAAC+TGG - chr4.1:13005073-13005092 None:intergenic 30.0%
ATTACTTCAGAGGAATGTAA+GGG + chr4.1:13005033-13005052 MS.gene27859:CDS 30.0%
ATTTGGAAGACCAATAATCT+TGG - chr4.1:13004987-13005006 None:intergenic 30.0%
GATCATAGATATTATCAGGT+TGG - chr4.1:13004256-13004275 None:intergenic 30.0%
GTTTGTGTAAGGAATAGAAT+TGG + chr4.1:13004500-13004519 MS.gene27859:CDS 30.0%
TTGTGTAAGGAATAGAATTG+GGG + chr4.1:13004502-13004521 MS.gene27859:CDS 30.0%
! AATGATTTCAGGCAAAGTTT+TGG - chr4.1:13004666-13004685 None:intergenic 30.0%
! ATATAATCATAGCTTGCACT+TGG - chr4.1:13004643-13004662 None:intergenic 30.0%
! CTTTTCTTACATTACTTCAG+AGG + chr4.1:13005023-13005042 MS.gene27859:CDS 30.0%
! TATTGTAGGAATTCCTGTTT+TGG + chr4.1:13004604-13004623 MS.gene27859:CDS 30.0%
! TCACAAACTGTTGATAAAAC+TGG + chr4.1:13004380-13004399 MS.gene27859:CDS 30.0%
! TTCACGTTTAGTAGATTTTG+TGG - chr4.1:13003724-13003743 None:intergenic 30.0%
!! TTATACTCTAAAACAAGCTC+TGG - chr4.1:13004050-13004069 None:intergenic 30.0%
!!! GAGCTTGTTTTAGAGTATAA+TGG + chr4.1:13004050-13004069 MS.gene27859:CDS 30.0%
AACAAACATAATGTTGGCCA+CGG - chr4.1:13005692-13005711 None:intergenic 35.0%
AACAGTTTGTGACATCCTAA+TGG - chr4.1:13004372-13004391 None:intergenic 35.0%
AATTTGCAGAAAAGCAGACA+TGG - chr4.1:13004768-13004787 None:intergenic 35.0%
ACAAGTAGTAAAATCAACCG+TGG - chr4.1:13004345-13004364 None:intergenic 35.0%
AGATCAACCATGGTTAAATC+TGG - chr4.1:13004203-13004222 None:intergenic 35.0%
CAACATATCCACTAATCTAG+CGG - chr4.1:13004876-13004895 None:intergenic 35.0%
CAATGCATTTGCTTATCATC+AGG - chr4.1:13004851-13004870 None:intergenic 35.0%
CATTACTTCAGAGGAATGTA+AGG + chr4.1:13005032-13005051 MS.gene27859:CDS 35.0%
GGCTAATAGTTTACATGAGT+TGG + chr4.1:13004706-13004725 MS.gene27859:CDS 35.0%
GTCTTGGTTATGTTTGTGTA+AGG + chr4.1:13004489-13004508 MS.gene27859:CDS 35.0%
GTTGATCATCAACATCAACA+TGG - chr4.1:13003767-13003786 None:intergenic 35.0%
TATGACCAAGGATTTCCTAA+TGG + chr4.1:13004937-13004956 MS.gene27859:CDS 35.0%
TGAAGAATCATCACTTGCAT+TGG + chr4.1:13003740-13003759 MS.gene27859:CDS 35.0%
TGCTGAAAGTAATATGACCA+AGG + chr4.1:13004925-13004944 MS.gene27859:CDS 35.0%
TGGTATTCCAGATTTAACCA+TGG + chr4.1:13004193-13004212 MS.gene27859:CDS 35.0%
TGTGAATGTTGATCTGCAAA+AGG + chr4.1:13005537-13005556 MS.gene27859:CDS 35.0%
TTACTACTTGTGAGTCCATT+AGG + chr4.1:13004354-13004373 MS.gene27859:CDS 35.0%
TTACTTCAGAGGAATGTAAG+GGG + chr4.1:13005034-13005053 MS.gene27859:CDS 35.0%
TTATACCAGCTAATGACTCA+AGG - chr4.1:13003953-13003972 None:intergenic 35.0%
TTGATCACCAACAACAACAA+TGG - chr4.1:13003910-13003929 None:intergenic 35.0%
! AATCAAAGTTAAAGGGGTGT+TGG - chr4.1:13004162-13004181 None:intergenic 35.0%
! GAACTTTGATTTCGTCCATT+AGG - chr4.1:13004955-13004974 None:intergenic 35.0%
! GAAGAAGAGAAGCTTTTTGA+GGG + chr4.1:13004551-13004570 MS.gene27859:CDS 35.0%
! TCAAGTACTGTGAATCTTGA+AGG + chr4.1:13005355-13005374 MS.gene27859:CDS 35.0%
! TGAAGAAGAGAAGCTTTTTG+AGG + chr4.1:13004550-13004569 MS.gene27859:CDS 35.0%
! TGACAGTGTTTGGAACTATT+TGG + chr4.1:13005084-13005103 MS.gene27859:CDS 35.0%
!! AATTTGCACAAGAGTACCAT+TGG + chr4.1:13003992-13004011 MS.gene27859:CDS 35.0%
!!! TGTCTGTTTTGTTTGTCTGT+TGG + chr4.1:13005752-13005771 MS.gene27859:CDS 35.0%
ACCGATGTTAACATCATCAG+CGG - chr4.1:13004471-13004490 None:intergenic 40.0%
ACTATTGGTGCAACAACAAG+TGG - chr4.1:13003795-13003814 None:intergenic 40.0%
ACTGTGAATCTTGAAGGACA+TGG + chr4.1:13005361-13005380 MS.gene27859:CDS 40.0%
AGGAGTCTCCTTCAATATCA+GGG + chr4.1:13005614-13005633 MS.gene27859:CDS 40.0%
ATCACCAACAACAACAATGG+TGG - chr4.1:13003907-13003926 None:intergenic 40.0%
ATCAGCCAATGATGCCAAAT+TGG - chr4.1:13004744-13004763 None:intergenic 40.0%
CAAGCAAGTTTCAACTGATG+AGG + chr4.1:13004073-13004092 MS.gene27859:CDS 40.0%
CGTATCAACATTGCCAAAGA+GGG + chr4.1:13003870-13003889 MS.gene27859:CDS 40.0%
CTTGAATGTTCTTTCTGCCA+CGG + chr4.1:13004325-13004344 MS.gene27859:CDS 40.0%
CTTGAGGAAGCCAAGATTAT+TGG + chr4.1:13004974-13004993 MS.gene27859:CDS 40.0%
GAACTGTTATCAGCAAATGG+TGG + chr4.1:13005571-13005590 MS.gene27859:CDS 40.0%
GATGATGTTAACATCGGTCT+TGG + chr4.1:13004473-13004492 MS.gene27859:CDS 40.0%
GCTGTTCTTGGCATGAAATT+TGG - chr4.1:13005004-13005023 None:intergenic 40.0%
GGAAAATTTGCCTGCCAATT+TGG + chr4.1:13004727-13004746 MS.gene27859:CDS 40.0%
GGACGAAATCAAAGTTCTTG+AGG + chr4.1:13004958-13004977 MS.gene27859:CDS 40.0%
GTACTCTTGTGCAAATTCCT+TGG - chr4.1:13003989-13004008 None:intergenic 40.0%
TAGATGCCCTAGAAAACCTA+CGG + chr4.1:13003847-13003866 MS.gene27859:CDS 40.0%
TATACTCCTGCAAGTACTCA+GGG - chr4.1:13005276-13005295 None:intergenic 40.0%
TCACCAACAACAACAATGGT+GGG - chr4.1:13003906-13003925 None:intergenic 40.0%
TCTATTCAACATGTGCTGCA+AGG + chr4.1:13005214-13005233 MS.gene27859:CDS 40.0%
TGCAAACTATTATCAGCTGC+AGG + chr4.1:13005141-13005160 MS.gene27859:CDS 40.0%
TGTCTCTTCCCTGATATTGA+AGG - chr4.1:13005625-13005644 None:intergenic 40.0%
TTAACCATGGTTGATCTTCC+AGG + chr4.1:13004206-13004225 MS.gene27859:CDS 40.0%
TTATACTCCTGCAAGTACTC+AGG - chr4.1:13005277-13005296 None:intergenic 40.0%
TTCGTCCATTAGGAAATCCT+TGG - chr4.1:13004945-13004964 None:intergenic 40.0%
! ACCGCTGATGATGTTAACAT+CGG + chr4.1:13004467-13004486 MS.gene27859:CDS 40.0%
! CTGTTGATAAAACTGGTCTG+AGG + chr4.1:13004387-13004406 MS.gene27859:CDS 40.0%
! GCTGATAATAGTTTGCAGAG+TGG - chr4.1:13005138-13005157 None:intergenic 40.0%
! TTTTGCAGCCTCATAACCAA+TGG - chr4.1:13004011-13004030 None:intergenic 40.0%
!! CAAGAGTACCATTGGTTATG+AGG + chr4.1:13004000-13004019 MS.gene27859:CDS 40.0%
!! TCAGCATTTGTTGTTGACTC+AGG + chr4.1:13005414-13005433 MS.gene27859:CDS 40.0%
!!! TTTTCTAGGGCATCTAGAAC+AGG - chr4.1:13003843-13003862 None:intergenic 40.0%
AATGCCTGGAAGATCAACCA+TGG - chr4.1:13004213-13004232 None:intergenic 45.0%
ACAAGCTTCTGTGCCAAAAC+AGG - chr4.1:13004620-13004639 None:intergenic 45.0%
AGCTAATGACTCAAGGACAC+TGG - chr4.1:13003946-13003965 None:intergenic 45.0%
AGGCAAGGCTAATTGCCTAT+TGG + chr4.1:13005449-13005468 MS.gene27859:CDS 45.0%
AGTGTCCTTGAGTCATTAGC+TGG + chr4.1:13003945-13003964 MS.gene27859:CDS 45.0%
ATGTAACCCTGAGTACTTGC+AGG + chr4.1:13005267-13005286 MS.gene27859:CDS 45.0%
ATTACTCGTGTCCCTGTACA+TGG + chr4.1:13004230-13004249 MS.gene27859:CDS 45.0%
ATTGGAAGATTGTGCTGAGG+AGG + chr4.1:13005467-13005486 MS.gene27859:CDS 45.0%
CCTATTGGAAGATTGTGCTG+AGG + chr4.1:13005464-13005483 MS.gene27859:CDS 45.0%
CCTCAGCACAATCTTCCAAT+AGG - chr4.1:13005467-13005486 None:intergenic 45.0%
CTGTTATCAGCAAATGGTGG+TGG + chr4.1:13005574-13005593 MS.gene27859:CDS 45.0%
GAGGAGTCTCCTTCAATATC+AGG + chr4.1:13005613-13005632 MS.gene27859:CDS 45.0%
GCGTATCAACATTGCCAAAG+AGG + chr4.1:13003869-13003888 MS.gene27859:CDS 45.0%
TGCTATAAACACAGCCACTG+AGG + chr4.1:13004109-13004128 MS.gene27859:CDS 45.0%
! AAAGCTGACAAAGCACCAGA+AGG + chr4.1:13004428-13004447 MS.gene27859:CDS 45.0%
! CAACAACAATGGTGGGAAGT+TGG - chr4.1:13003899-13003918 None:intergenic 45.0%
! CTTCCCACCATTGTTGTTGT+TGG + chr4.1:13003900-13003919 MS.gene27859:CDS 45.0%
! GATACGCCGTAGGTTTTCTA+GGG - chr4.1:13003856-13003875 None:intergenic 45.0%
! TAGCCTTGCCTTCAAGTCAA+AGG - chr4.1:13005440-13005459 None:intergenic 45.0%
! TCTAAAACAAGCTCTGGCTG+TGG - chr4.1:13004044-13004063 None:intergenic 45.0%
! TGATACGCCGTAGGTTTTCT+AGG - chr4.1:13003857-13003876 None:intergenic 45.0%
!! GTTGTTGGTGATCAATCGTC+TGG + chr4.1:13003915-13003934 MS.gene27859:CDS 45.0%
!! TATCAGGTTGGCCATGTACA+GGG - chr4.1:13004244-13004263 None:intergenic 45.0%
!! TGTGCTGCAAGGTGTTGAAA+TGG + chr4.1:13005225-13005244 MS.gene27859:CDS 45.0%
!! TTATCAGGTTGGCCATGTAC+AGG - chr4.1:13004245-13004264 None:intergenic 45.0%
ATAAGTTTGCCACGTGGCCA+AGG + chr4.1:13003969-13003988 MS.gene27859:CDS 50.0%
GAGAGAAAGCAAAGAGACCG+TGG + chr4.1:13005672-13005691 MS.gene27859:CDS 50.0%
GCATTGCACCGCTAGATTAG+TGG + chr4.1:13004865-13004884 MS.gene27859:CDS 50.0%
GCCAATGATGCCAAATTGGC+AGG - chr4.1:13004740-13004759 None:intergenic 50.0%
GCCTGCCAATTTGGCATCAT+TGG + chr4.1:13004736-13004755 MS.gene27859:CDS 50.0%
GCTGGTATAAGTTTGCCACG+TGG + chr4.1:13003963-13003982 MS.gene27859:CDS 50.0%
GGAAGAGACAGAAGTTGAGC+AGG + chr4.1:13005635-13005654 MS.gene27859:CDS 50.0%
GGGAAGTTGGATTCCCTCTT+TGG - chr4.1:13003886-13003905 None:intergenic 50.0%
GGTGCAACAACAAGTGGTTG+AGG - chr4.1:13003789-13003808 None:intergenic 50.0%
TGGCAATGTTGATACGCCGT+AGG - chr4.1:13003866-13003885 None:intergenic 50.0%
! ACCTTTTCCAGCAAGCCTTC+TGG - chr4.1:13004446-13004465 None:intergenic 50.0%
!! ACCAGAAGGCTTGCTGGAAA+AGG + chr4.1:13004442-13004461 MS.gene27859:CDS 50.0%
!! AGGCCTTTGACTTGAAGGCA+AGG + chr4.1:13005434-13005453 MS.gene27859:CDS 50.0%
!! AGGGGTGTTGGAAATCCCTT+TGG - chr4.1:13004150-13004169 None:intergenic 50.0%
!! CAAATGGTGGTGGTGTTGAG+AGG + chr4.1:13005584-13005603 MS.gene27859:CDS 50.0%
!! GACTCAGGCCTTTGACTTGA+AGG + chr4.1:13005429-13005448 MS.gene27859:CDS 50.0%
ACAGGGACACGAGTAATGCC+TGG - chr4.1:13004227-13004246 None:intergenic 55.0%
GTGCAAATTCCTTGGCCACG+TGG - chr4.1:13003981-13004000 None:intergenic 55.0%
! ACAAGCTCTGGCTGTGGAAG+TGG - chr4.1:13004038-13004057 None:intergenic 55.0%
! CAAAGCACCAGAAGGCTTGC+TGG + chr4.1:13004436-13004455 MS.gene27859:CDS 55.0%
! TGGTGTTGAGAGGCTGCTTG+AGG + chr4.1:13005594-13005613 MS.gene27859:CDS 55.0%
!! AGCTTGCTGGCACTGCCAAA+GGG + chr4.1:13004132-13004151 MS.gene27859:CDS 55.0%
ACAGCCACTGAGGAGCTTGC+TGG + chr4.1:13004119-13004138 MS.gene27859:CDS 60.0%
AGTGCCAGCAAGCTCCTCAG+TGG - chr4.1:13004126-13004145 None:intergenic 60.0%
GCTCTGGCTGTGGAAGTGGA+TGG - chr4.1:13004034-13004053 None:intergenic 60.0%
!! GAGCTTGCTGGCACTGCCAA+AGG + chr4.1:13004131-13004150 MS.gene27859:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 13003699 13005783 13003699 ID=MS.gene27859
chr4.1 mRNA 13003699 13005783 13003699 ID=MS.gene27859.t1;Parent=MS.gene27859
chr4.1 exon 13003699 13005783 13003699 ID=MS.gene27859.t1.exon1;Parent=MS.gene27859.t1
chr4.1 CDS 13003699 13005783 13003699 ID=cds.MS.gene27859.t1;Parent=MS.gene27859.t1
Gene Sequence

>MS.gene27859

ATGGCTTTTGGAAAGAAGAGTTCCACAAAATCTACTAAACGTGAAGAATCATCACTTGCATTGGTCCATGTTGATGTTGATGATCAACCTCAACCACTTGTTGTTGCACCAATAGTATCATCTTACAATGAGAAGATACGTCCTGTTCTAGATGCCCTAGAAAACCTACGGCGTATCAACATTGCCAAAGAGGGAATCCAACTTCCCACCATTGTTGTTGTTGGTGATCAATCGTCTGGCAAGTCCAGTGTCCTTGAGTCATTAGCTGGTATAAGTTTGCCACGTGGCCAAGGAATTTGCACAAGAGTACCATTGGTTATGAGGCTGCAAAACCATCCACTTCCACAGCCAGAGCTTGTTTTAGAGTATAATGGCAAGCAAGTTTCAACTGATGAGGAAAATGTATCTGATGCTATAAACACAGCCACTGAGGAGCTTGCTGGCACTGCCAAAGGGATTTCCAACACCCCTTTAACTTTGATTGTTAAGAAAAATGGTATTCCAGATTTAACCATGGTTGATCTTCCAGGCATTACTCGTGTCCCTGTACATGGCCAACCTGATAATATCTATGATCAAATTAAGGATATTATCATGGAGTACATAACACCAGAAGAAAGTATTATCTTGAATGTTCTTTCTGCCACGGTTGATTTTACTACTTGTGAGTCCATTAGGATGTCACAAACTGTTGATAAAACTGGTCTGAGGACACTTGCTGTCGTAACAAAAGCTGACAAAGCACCAGAAGGCTTGCTGGAAAAGGTAACCGCTGATGATGTTAACATCGGTCTTGGTTATGTTTGTGTAAGGAATAGAATTGGGGATGAGTCTTATGAAGATGCTAGAAATGAAGAAGAGAAGCTTTTTGAGGGACATTCACTTCTTTCCAAAATAGATAAATCTATTGTAGGAATTCCTGTTTTGGCACAGAAGCTTGTCCAAGTGCAAGCTATGATTATATCCAAAACTTTGCCTGAAATCATTAAGAAGATTAATGAGAAGTTGGCTAATAGTTTACATGAGTTGGAAAATTTGCCTGCCAATTTGGCATCATTGGCTGATGCCATGTCTGCTTTTCTGCAAATTATCTCTTTGTCAAGAGACTCTCTTAAAAAGATTCTTCTGATAGGGGAGTTTGAAGAGTATCCTGATGATAAGCAAATGCATTGCACCGCTAGATTAGTGGATATGTTGAATTCATATGCAACTGAACTTCAAAACTGTGCTGAAAGTAATATGACCAAGGATTTCCTAATGGACGAAATCAAAGTTCTTGAGGAAGCCAAGATTATTGGTCTTCCAAATTTCATGCCAAGAACAGCTTTTCTTACATTACTTCAGAGGAATGTAAGGGGCGTTTCTTATTTGCCAGTTAATTTTGTTGACAGTGTTTGGAACTATTTGGAAACTGTTGTTACTTCTGTTTTGAATCGCCACTCTGCAAACTATTATCAGCTGCAGGTTTCAATTCGTAGAGCTGCTGAGCATCTTATTGCCAAGAAGAAAAAGAATTCTATTCAACATGTGCTGCAAGGTGTTGAAATGGAGAAGCTTACAGACTACACATGTAACCCTGAGTACTTGCAGGAGTATAACAAGCTGATGTCTAATCAAGAAGCGTTTTTGAAAGAAGTGTTGAATGCAAATAGAGAATCAAGTACTGTGAATCTTGAAGGACATGGTGAGATTGAAGTTGCTCATCTGAAGAACTATCAGCATTTGTTGTTGACTCAGGCCTTTGACTTGAAGGCAAGGCTAATTGCCTATTGGAAGATTGTGCTGAGGAGGCTCATTGATGTTATTGCTTTGCATTTGATGTTAAGCATCAATGAGCTTGTGAATGTTGATCTGCAAAAGGAGATCTGTAATGAACTGTTATCAGCAAATGGTGGTGGTGTTGAGAGGCTGCTTGAGGAGTCTCCTTCAATATCAGGGAAGAGACAGAAGTTGAGCAGGAGTGTGACAGTTTTGAGAGAAAGCAAAGAGACCGTGGCCAACATTATGTTTGTTTTAGTTTGTTTTTATCTATCTTGCAAGTGTCCTTTTGTCATGTCTGTTTTGTTTGTCTGTTGGAACTTTTAG
Protein sequence

>MS.gene27859.t1

MAFGKKSSTKSTKREESSLALVHVDVDDQPQPLVVAPIVSSYNEKIRPVLDALENLRRINIAKEGIQLPTIVVVGDQSSGKSSVLESLAGISLPRGQGICTRVPLVMRLQNHPLPQPELVLEYNGKQVSTDEENVSDAINTATEELAGTAKGISNTPLTLIVKKNGIPDLTMVDLPGITRVPVHGQPDNIYDQIKDIIMEYITPEESIILNVLSATVDFTTCESIRMSQTVDKTGLRTLAVVTKADKAPEGLLEKVTADDVNIGLGYVCVRNRIGDESYEDARNEEEKLFEGHSLLSKIDKSIVGIPVLAQKLVQVQAMIISKTLPEIIKKINEKLANSLHELENLPANLASLADAMSAFLQIISLSRDSLKKILLIGEFEEYPDDKQMHCTARLVDMLNSYATELQNCAESNMTKDFLMDEIKVLEEAKIIGLPNFMPRTAFLTLLQRNVRGVSYLPVNFVDSVWNYLETVVTSVLNRHSANYYQLQVSIRRAAEHLIAKKKKNSIQHVLQGVEMEKLTDYTCNPEYLQEYNKLMSNQEAFLKEVLNANRESSTVNLEGHGEIEVAHLKNYQHLLLTQAFDLKARLIAYWKIVLRRLIDVIALHLMLSINELVNVDLQKEICNELLSANGGGVERLLEESPSISGKRQKLSRSVTVLRESKETVANIMFVLVCFYLSCKCPFVMSVLFVCWNF