AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28097

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28097.t1 MTR_8g090330 99.180 366 3 0 1 366 1 366 0.0 761
MS.gene28097.t1 MTR_8g090330 99.310 290 2 0 1 290 1 290 0.0 605
MS.gene28097.t1 MTR_3g074180 62.259 363 126 3 3 359 4 361 6.52e-162 458
MS.gene28097.t1 MTR_5g063080 67.093 313 101 2 50 362 66 376 5.97e-153 436
MS.gene28097.t1 MTR_4g101600 52.646 378 156 4 8 365 16 390 4.26e-139 401
MS.gene28097.t1 MTR_4g036090 53.390 354 137 4 32 363 39 386 3.19e-131 381
MS.gene28097.t1 MTR_3g008500 52.762 362 152 4 17 359 25 386 1.81e-130 379
MS.gene28097.t1 MTR_4g036090 53.869 336 127 4 32 345 39 368 8.40e-123 362
MS.gene28097.t1 MTR_3g091010 52.759 290 127 2 79 358 66 355 6.92e-109 323
MS.gene28097.t1 MTR_8g027765 47.797 295 144 2 74 358 17 311 3.79e-102 304
MS.gene28097.t1 MTR_4g036685 44.490 245 101 5 77 300 25 255 9.82e-58 189
MS.gene28097.t1 MTR_4g128870 51.351 111 32 4 97 207 1 89 1.09e-24 96.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28097.t1 AT5G65140 70.811 370 92 6 1 357 1 367 0.0 535
MS.gene28097.t1 AT5G10100 69.315 365 104 4 4 361 16 379 0.0 527
MS.gene28097.t1 AT5G10100 69.315 365 104 4 4 361 5 368 0.0 526
MS.gene28097.t1 AT5G10100 68.966 348 100 4 4 344 5 351 4.12e-178 499
MS.gene28097.t1 AT4G39770 66.387 357 100 4 3 357 6 344 3.18e-174 488
MS.gene28097.t1 AT5G10100 64.658 365 101 5 4 361 16 359 1.40e-169 477
MS.gene28097.t1 AT2G22190 69.876 322 90 3 38 358 35 350 4.73e-167 471
MS.gene28097.t1 AT5G65140 70.245 326 81 6 1 313 1 323 9.27e-167 469
MS.gene28097.t1 AT4G39770 65.797 345 98 4 3 345 6 332 1.49e-165 466
MS.gene28097.t1 AT1G35910 64.721 377 111 7 1 363 1 369 5.16e-164 463
MS.gene28097.t1 AT2G22190 69.677 310 87 3 38 346 35 338 1.36e-159 451
MS.gene28097.t1 AT1G78090 61.662 373 126 6 1 359 1 370 5.10e-156 443
MS.gene28097.t1 AT1G78090 61.838 359 120 6 1 345 1 356 2.31e-149 426
MS.gene28097.t1 AT5G65140 68.647 303 79 6 1 290 1 300 1.33e-148 422
MS.gene28097.t1 AT1G22210 61.300 323 113 3 43 359 1 317 7.65e-138 395
MS.gene28097.t1 AT5G51460 54.545 341 132 6 35 358 48 382 3.07e-129 375
MS.gene28097.t1 AT5G51460 54.545 341 132 6 35 358 48 382 3.07e-129 375
MS.gene28097.t1 AT5G51460 54.386 342 132 7 35 358 48 383 1.49e-127 371
MS.gene28097.t1 AT5G51460 54.386 342 132 7 35 358 48 383 1.49e-127 371
MS.gene28097.t1 AT5G51460 54.386 342 132 7 35 358 48 383 1.49e-127 371
MS.gene28097.t1 AT4G22590 53.198 344 141 4 31 357 29 369 6.38e-124 362
MS.gene28097.t1 AT4G12430 56.522 322 127 3 53 362 45 365 2.85e-120 352

Find 86 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACTCACTTTGTTGTATT+TGG 0.157241 4.4:-10266340 None:intergenic
AGATATGTTTGAACAAATTA+TGG 0.199793 4.4:+10265877 MS.gene28097:CDS
AGATTCGTCCTGCAATTAAA+TGG 0.218209 4.4:+10267093 MS.gene28097:CDS
GCTACCATTCATTCCGGTTT+TGG 0.246623 4.4:-10265465 None:intergenic
TAGCTGAAGAATACAATTCT+TGG 0.262718 4.4:+10265657 MS.gene28097:CDS
TTTCCTACTGCAATTGTTAC+TGG 0.272515 4.4:+10266124 MS.gene28097:CDS
AGAGACATAGATCAAGGTTT+TGG 0.278672 4.4:+10267704 MS.gene28097:CDS
CCTTTGTCCCATTTAATTGC+AGG 0.303034 4.4:-10267101 None:intergenic
AGTCATGGCATGGATATTAA+AGG 0.325492 4.4:+10266303 MS.gene28097:CDS
GAAATTATGCTTACCTCATT+AGG 0.325958 4.4:-10267782 None:intergenic
GATGTATTTCCTGTTTATAT+TGG 0.349971 4.4:+10267487 MS.gene28097:CDS
TTCTTCAAAATTCTCCTCCT+TGG 0.362292 4.4:-10265545 None:intergenic
GGAGAAGATTTGACATGAGT+TGG 0.374230 4.4:-10265629 None:intergenic
TATGGATTTCCTTCAGCGTT+TGG 0.380303 4.4:+10267880 MS.gene28097:CDS
TTGAAGAATCTTGAAATCAA+TGG 0.381109 4.4:+10265560 MS.gene28097:CDS
CTTTAGATGTGTTGATGAAA+AGG 0.396311 4.4:+10266738 MS.gene28097:CDS
TACACCCTAGAGCGTGATCT+TGG 0.396817 4.4:-10267923 None:intergenic
CCTTGTGTTAGACGAAGCTT+CGG 0.397569 4.4:-10266896 None:intergenic
TACACAGAGATGGGTTGAAT+TGG 0.408365 4.4:+10266841 MS.gene28097:intron
AGTACCGCTACCATTCATTC+CGG 0.416696 4.4:-10265471 None:intergenic
CTATCATCACCAATATAAAC+AGG 0.428430 4.4:-10267496 None:intergenic
TATGCAGCAGCTGTGTCCTT+TGG 0.429413 4.4:-10267746 None:intergenic
CTTGTGTTAGACGAAGCTTC+GGG 0.443939 4.4:-10266895 None:intergenic
GTGACTAACCTCATTTATCA+TGG 0.449499 4.4:-10266539 None:intergenic
CCGTGCTTTCATGTCTGAAT+CGG 0.453757 4.4:+10265976 MS.gene28097:CDS
AACCGATGAAGATGCATTCA+AGG 0.456478 4.4:+10267519 MS.gene28097:CDS
TATCATGGGAACAAATTCTC+TGG 0.470973 4.4:-10266524 None:intergenic
TCAGACATGAAAGCACGGTC+GGG 0.472310 4.4:-10265971 None:intergenic
CTATATTATGCTGGAAGTCA+TGG 0.475549 4.4:+10266288 MS.gene28097:CDS
ACAAATTATGGATGCTGCAA+AGG 0.481613 4.4:+10265889 MS.gene28097:CDS
TCCTTCAGCGTTTGGTGGAG+TGG 0.484492 4.4:+10267888 MS.gene28097:CDS
GTCATGGCATGGATATTAAA+GGG 0.494286 4.4:+10266304 MS.gene28097:CDS
TTGGATGAACTATATTATGC+TGG 0.495236 4.4:+10266279 MS.gene28097:CDS
CGGTCGGGGTCATCCACAAT+TGG 0.498926 4.4:-10265956 None:intergenic
CATCTCCAAGATCACGCTCT+AGG 0.500203 4.4:+10267918 MS.gene28097:CDS
TTGTATTCTTCAGCTAAAGA+AGG 0.501331 4.4:-10265650 None:intergenic
GATTCGTCCTGCAATTAAAT+GGG 0.502618 4.4:+10267094 MS.gene28097:CDS
GAACTTGTGGTTCTCAACTC+TGG 0.503615 4.4:-10266701 None:intergenic
GAGACCAAAACCGGAATGAA+TGG 0.513958 4.4:+10265461 MS.gene28097:CDS
ATTTGTTCCCATGATAAATG+AGG 0.514951 4.4:+10266531 MS.gene28097:CDS
ATGTATCCACCGGGTGCTGC+CGG 0.516770 4.4:-10265515 None:intergenic
ATCTCCAAGATCACGCTCTA+GGG 0.517903 4.4:+10267919 MS.gene28097:CDS
TGGTTCTCAACTCTGGCACC+AGG 0.519019 4.4:-10266694 None:intergenic
CAAATTATGGATGCTGCAAA+GGG 0.526052 4.4:+10265890 MS.gene28097:CDS
TGCCGGTGGCTTCTGTGCCA+CGG 0.527210 4.4:-10265498 None:intergenic
CAGAAGCCACCGGCAGCACC+CGG 0.527998 4.4:+10265506 MS.gene28097:CDS
AAATTAAGAGACATAGATCA+AGG 0.529862 4.4:+10267698 MS.gene28097:CDS
ATGGGAACAAATTCTCTGGC+AGG 0.537434 4.4:-10266520 None:intergenic
TACCTTGAATGCATCTTCAT+CGG 0.539742 4.4:-10267521 None:intergenic
GGATTTCCTTCAGCGTTTGG+TGG 0.542635 4.4:+10267883 MS.gene28097:CDS
TATCCACCGGGTGCTGCCGG+TGG 0.542892 4.4:-10265512 None:intergenic
TTCAGACATGAAAGCACGGT+CGG 0.542972 4.4:-10265972 None:intergenic
AGGAAATACATCGTTACAGT+TGG 0.546340 4.4:-10267476 None:intergenic
TCCACTCCACCAAACGCTGA+AGG 0.548294 4.4:-10267889 None:intergenic
TGGTACTTTGTCACCAATTG+TGG 0.553666 4.4:+10265943 MS.gene28097:CDS
GAGAAAACAAAATCAACTCC+TGG 0.555984 4.4:+10266676 MS.gene28097:CDS
CGAACAGCTTCGCCATCCAT+CGG 0.563670 4.4:+10265850 MS.gene28097:intron
GTGGATACATTCACATTCCA+AGG 0.565455 4.4:+10265528 MS.gene28097:CDS
GTTGTGTCTGAGACCAAAAC+CGG 0.569541 4.4:+10265452 MS.gene28097:CDS
GAACAGAGGTGCAGAACTTG+TGG 0.573976 4.4:-10266714 None:intergenic
ACATATCTAATGCCGATGGA+TGG 0.584345 4.4:-10265862 None:intergenic
CTCTTTACAAGAGCCTAATG+AGG 0.589522 4.4:+10267769 MS.gene28097:CDS
TCTAGTCTCTAAGTTTCCAA+AGG 0.593019 4.4:+10267730 MS.gene28097:CDS
AAGAATCTTGAAATCAATGG+AGG 0.594315 4.4:+10265563 MS.gene28097:CDS
TACCGTGGCACAGAAGCCAC+CGG 0.596430 4.4:+10265496 MS.gene28097:CDS
TCGTCTAACACAAGGAAGGA+AGG 0.605150 4.4:+10266904 MS.gene28097:CDS
TGACTAACCTCATTTATCAT+GGG 0.605779 4.4:-10266538 None:intergenic
AATTAAATGGGACAAAGGCA+AGG 0.608301 4.4:+10267106 MS.gene28097:CDS
CAGACATGAAAGCACGGTCG+GGG 0.619472 4.4:-10265970 None:intergenic
TGGAATGTGAATGTATCCAC+CGG 0.620563 4.4:-10265525 None:intergenic
TTATGCTGGAAGTCATGGCA+TGG 0.621883 4.4:+10266293 MS.gene28097:CDS
CTTCCAGTAACAATTGCAGT+AGG 0.624355 4.4:-10266127 None:intergenic
TCAAACATATCTAATGCCGA+TGG 0.625537 4.4:-10265866 None:intergenic
GAGGACAAAGAATCAATACA+TGG 0.625642 4.4:+10265582 MS.gene28097:CDS
CCTGCAATTAAATGGGACAA+AGG 0.636970 4.4:+10267101 MS.gene28097:CDS
CCGAAGCTTCGTCTAACACA+AGG 0.637863 4.4:+10266896 MS.gene28097:CDS
AAGCCACCGGCAGCACCCGG+TGG 0.650481 4.4:+10265509 MS.gene28097:CDS
TTTGTAGAATAACAAAGCAG+AGG 0.655035 4.4:+10266483 MS.gene28097:intron
AGCTTCGTCTAACACAAGGA+AGG 0.657256 4.4:+10266900 MS.gene28097:CDS
AAAACCGGAATGAATGGTAG+CGG 0.657999 4.4:+10265467 MS.gene28097:CDS
TGGTAGCGGTACTATTACCG+TGG 0.662829 4.4:+10265481 MS.gene28097:CDS
GAAGATGACTCAAAAGAATG+TGG 0.668118 4.4:+10265430 None:intergenic
CCGATTCAGACATGAAAGCA+CGG 0.680880 4.4:-10265976 None:intergenic
AACACATCTAAAGTGAACAG+AGG 0.711327 4.4:-10266728 None:intergenic
GGAATGTGAATGTATCCACC+GGG 0.725876 4.4:-10265524 None:intergenic
GATACATTCACATTCCAAGG+AGG 0.760013 4.4:+10265531 MS.gene28097:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAACAGATTTATTTAA+AGG - chr4.4:10266034-10266053 None:intergenic 10.0%
!! ATTTCAAAAAAGATAAAAAA+AGG - chr4.4:10266198-10266217 None:intergenic 10.0%
!! TTATTAATTTAATATCATTC+AGG + chr4.4:10267856-10267875 MS.gene28097:intron 10.0%
!! TTCATTTAGAAATAAAAAAT+TGG - chr4.4:10267574-10267593 None:intergenic 10.0%
!! TTTCAAAAAAGATAAAAAAA+GGG - chr4.4:10266197-10266216 None:intergenic 10.0%
!!! TTCTAAAAATTAATTAACAT+TGG + chr4.4:10266454-10266473 MS.gene28097:intron 10.0%
!! AAAAAAACAATTAAGATCAT+TGG - chr4.4:10267548-10267567 None:intergenic 15.0%
!! AAATTAATTAACATTGGTAA+TGG + chr4.4:10266460-10266479 MS.gene28097:intron 15.0%
!! ATCAGTAAAACATATATTTA+AGG - chr4.4:10267836-10267855 None:intergenic 15.0%
!! ATTTAGAAATAAAAAATTGG+AGG - chr4.4:10267571-10267590 None:intergenic 15.0%
!! TTTATTAGTTAGAAAATTGA+TGG - chr4.4:10267437-10267456 None:intergenic 15.0%
!!! TTAGTTTTTGTTTATAAAAC+TGG + chr4.4:10265999-10266018 MS.gene28097:intron 15.0%
!!! TTCTTTATAATCAACTTTTT+TGG + chr4.4:10266944-10266963 MS.gene28097:intron 15.0%
!! AACAACACGTATAAATAATA+AGG - chr4.4:10265825-10265844 None:intergenic 20.0%
!! AATATATATTTAGAAACGCA+TGG - chr4.4:10267399-10267418 None:intergenic 20.0%
!! AGAATTAAACATAATTGACT+TGG - chr4.4:10267617-10267636 None:intergenic 20.0%
!! AGATATGTTTGAACAAATTA+TGG + chr4.4:10265877-10265896 MS.gene28097:CDS 20.0%
!! TTAAAAAATATACTGTACTG+TGG - chr4.4:10266389-10266408 None:intergenic 20.0%
!!! AAAACAGATTGTTATGTTTT+TGG + chr4.4:10265913-10265932 MS.gene28097:CDS 20.0%
!!! ATTTAATATCATTCAGGTTA+TGG + chr4.4:10267862-10267881 MS.gene28097:intron 20.0%
!!! TTATAATCAACTTTTTTGGA+GGG + chr4.4:10266948-10266967 MS.gene28097:intron 20.0%
!!! TTTATAATCAACTTTTTTGG+AGG + chr4.4:10266947-10266966 MS.gene28097:intron 20.0%
!!! TTTTACCTTTGTTAATTTTC+AGG + chr4.4:10266636-10266655 MS.gene28097:intron 20.0%
! AAATTAAGAGACATAGATCA+AGG + chr4.4:10267698-10267717 MS.gene28097:CDS 25.0%
! CAATGTCAATTGTTTAATAC+AGG + chr4.4:10267064-10267083 MS.gene28097:intron 25.0%
! CAATTGTTTAATACAGGTAT+TGG + chr4.4:10267070-10267089 MS.gene28097:intron 25.0%
! GATGTATTTCCTGTTTATAT+TGG + chr4.4:10267487-10267506 MS.gene28097:CDS 25.0%
! GTGTGATTTAATTAGTTCAA+AGG + chr4.4:10266994-10267013 MS.gene28097:intron 25.0%
! TATCACATATATATCAGATG+AGG + chr4.4:10266074-10266093 MS.gene28097:intron 25.0%
! TTGAAGAATCTTGAAATCAA+TGG + chr4.4:10265560-10265579 MS.gene28097:CDS 25.0%
!! ACTAATAAATCGTTTTCAAC+AGG + chr4.4:10267448-10267467 MS.gene28097:intron 25.0%
!! CCAAAATTTTGAACATATAC+TGG + chr4.4:10265752-10265771 MS.gene28097:intron 25.0%
!! CCAGTATATGTTCAAAATTT+TGG - chr4.4:10265755-10265774 None:intergenic 25.0%
!! GTATATGTTCAAAATTTTGG+TGG - chr4.4:10265752-10265771 None:intergenic 25.0%
!! TAATTCTTTGTAGACTGTTT+TGG + chr4.4:10267630-10267649 MS.gene28097:intron 25.0%
!! TTTACTCACTTTGTTGTATT+TGG - chr4.4:10266343-10266362 None:intergenic 25.0%
!! TTTTTCTGCAAACAATTTTC+AGG + chr4.4:10266239-10266258 MS.gene28097:intron 25.0%
!!! AATTTTAAGACCTTATTCCA+CGG - chr4.4:10266428-10266447 None:intergenic 25.0%
!!! AGTGTATATTTTTAATGGTG+GGG + chr4.4:10266971-10266990 MS.gene28097:intron 25.0%
!!! GAGTGTATATTTTTAATGGT+GGG + chr4.4:10266970-10266989 MS.gene28097:intron 25.0%
!!! GTTATGTTTTTGGATTATGA+TGG + chr4.4:10265923-10265942 MS.gene28097:CDS 25.0%
AAGAATCTTGAAATCAATGG+AGG + chr4.4:10265563-10265582 MS.gene28097:CDS 30.0%
ACCATTATGACAGAATTAAG+AGG + chr4.4:10267318-10267337 MS.gene28097:intron 30.0%
ATTTGTTCCCATGATAAATG+AGG + chr4.4:10266531-10266550 MS.gene28097:CDS 30.0%
CCATTATGACAGAATTAAGA+GGG + chr4.4:10267319-10267338 MS.gene28097:intron 30.0%
CTATCATCACCAATATAAAC+AGG - chr4.4:10267499-10267518 None:intergenic 30.0%
GAAATTATGCTTACCTCATT+AGG - chr4.4:10267785-10267804 None:intergenic 30.0%
GTACACCTGAAAATTAACAA+AGG - chr4.4:10266644-10266663 None:intergenic 30.0%
TAGCTGAAGAATACAATTCT+TGG + chr4.4:10265657-10265676 MS.gene28097:CDS 30.0%
TATAAATATGCTACTGCTCA+TGG + chr4.4:10267264-10267283 MS.gene28097:intron 30.0%
TGACTAACCTCATTTATCAT+GGG - chr4.4:10266541-10266560 None:intergenic 30.0%
TGTCATAATGGTCAAATACT+TGG - chr4.4:10267310-10267329 None:intergenic 30.0%
TGTTTATTGTACACAGAGAT+GGG + chr4.4:10266832-10266851 MS.gene28097:intron 30.0%
TTGGATGAACTATATTATGC+TGG + chr4.4:10266279-10266298 MS.gene28097:CDS 30.0%
TTGTATTCTTCAGCTAAAGA+AGG - chr4.4:10265653-10265672 None:intergenic 30.0%
TTGTTTATTGTACACAGAGA+TGG + chr4.4:10266831-10266850 MS.gene28097:intron 30.0%
TTTGTAGAATAACAAAGCAG+AGG + chr4.4:10266483-10266502 MS.gene28097:intron 30.0%
! CTTTAGATGTGTTGATGAAA+AGG + chr4.4:10266738-10266757 MS.gene28097:CDS 30.0%
! TTGTATTTGGACTCTTTTGT+TGG - chr4.4:10266330-10266349 None:intergenic 30.0%
!! GAGGGAGTGTATATTTTTAA+TGG + chr4.4:10266966-10266985 MS.gene28097:intron 30.0%
!! GGAGTGTATATTTTTAATGG+TGG + chr4.4:10266969-10266988 MS.gene28097:intron 30.0%
!! GTGTATATTTTTAATGGTGG+GGG + chr4.4:10266972-10266991 MS.gene28097:intron 30.0%
AATTAAATGGGACAAAGGCA+AGG + chr4.4:10267106-10267125 MS.gene28097:CDS 35.0%
ACAAATTATGGATGCTGCAA+AGG + chr4.4:10265889-10265908 MS.gene28097:CDS 35.0%
AGATTCGTCCTGCAATTAAA+TGG + chr4.4:10267093-10267112 MS.gene28097:CDS 35.0%
AGGAAATACATCGTTACAGT+TGG - chr4.4:10267479-10267498 None:intergenic 35.0%
AGTCATGGCATGGATATTAA+AGG + chr4.4:10266303-10266322 MS.gene28097:CDS 35.0%
AGTTCAAAGGTGATTATGAC+TGG + chr4.4:10267007-10267026 MS.gene28097:intron 35.0%
CAAATTATGGATGCTGCAAA+GGG + chr4.4:10265890-10265909 MS.gene28097:CDS 35.0%
CCCTCTTAATTCTGTCATAA+TGG - chr4.4:10267322-10267341 None:intergenic 35.0%
CTATATTATGCTGGAAGTCA+TGG + chr4.4:10266288-10266307 MS.gene28097:CDS 35.0%
GAGAAAACAAAATCAACTCC+TGG + chr4.4:10266676-10266695 MS.gene28097:CDS 35.0%
GAGGACAAAGAATCAATACA+TGG + chr4.4:10265582-10265601 MS.gene28097:CDS 35.0%
GATTCGTCCTGCAATTAAAT+GGG + chr4.4:10267094-10267113 MS.gene28097:CDS 35.0%
GTCATGGCATGGATATTAAA+GGG + chr4.4:10266304-10266323 MS.gene28097:CDS 35.0%
GTGACTAACCTCATTTATCA+TGG - chr4.4:10266542-10266561 None:intergenic 35.0%
GTTCAAAGGTGATTATGACT+GGG + chr4.4:10267008-10267027 MS.gene28097:intron 35.0%
TACCTTGAATGCATCTTCAT+CGG - chr4.4:10267524-10267543 None:intergenic 35.0%
TATCATGGGAACAAATTCTC+TGG - chr4.4:10266527-10266546 None:intergenic 35.0%
TCAAACATATCTAATGCCGA+TGG - chr4.4:10265869-10265888 None:intergenic 35.0%
TCTAGTCTCTAAGTTTCCAA+AGG + chr4.4:10267730-10267749 MS.gene28097:CDS 35.0%
TGACTCTAGCAAAAATTCGA+GGG - chr4.4:10267132-10267151 None:intergenic 35.0%
TTCTTCAAAATTCTCCTCCT+TGG - chr4.4:10265548-10265567 None:intergenic 35.0%
TTTCCTACTGCAATTGTTAC+TGG + chr4.4:10266124-10266143 MS.gene28097:CDS 35.0%
! AACACATCTAAAGTGAACAG+AGG - chr4.4:10266731-10266750 None:intergenic 35.0%
! AGAGACATAGATCAAGGTTT+TGG + chr4.4:10267704-10267723 MS.gene28097:CDS 35.0%
! GAATTTTTGCTAGAGTCACT+TGG + chr4.4:10267134-10267153 MS.gene28097:CDS 35.0%
! GGTGTACAATTTTGTTCGTT+TGG + chr4.4:10266260-10266279 MS.gene28097:intron 35.0%
AAAACCGGAATGAATGGTAG+CGG + chr4.4:10265467-10265486 MS.gene28097:CDS 40.0%
AACCGATGAAGATGCATTCA+AGG + chr4.4:10267519-10267538 MS.gene28097:CDS 40.0%
ACATATCTAATGCCGATGGA+TGG - chr4.4:10265865-10265884 None:intergenic 40.0%
ATGAGTAAACTAATCCCACG+AGG - chr4.4:10267356-10267375 None:intergenic 40.0%
ATTTGACCTATATAGTGCCG+TGG + chr4.4:10266408-10266427 MS.gene28097:intron 40.0%
CCTGCAATTAAATGGGACAA+AGG + chr4.4:10267101-10267120 MS.gene28097:CDS 40.0%
CCTTTGTCCCATTTAATTGC+AGG - chr4.4:10267104-10267123 None:intergenic 40.0%
CTATATAGTGCCGTGGAATA+AGG + chr4.4:10266415-10266434 MS.gene28097:intron 40.0%
CTCTTTACAAGAGCCTAATG+AGG + chr4.4:10267769-10267788 MS.gene28097:CDS 40.0%
CTTATTCCACGGCACTATAT+AGG - chr4.4:10266417-10266436 None:intergenic 40.0%
CTTCCAGTAACAATTGCAGT+AGG - chr4.4:10266130-10266149 None:intergenic 40.0%
GATACATTCACATTCCAAGG+AGG + chr4.4:10265531-10265550 MS.gene28097:CDS 40.0%
GGAGAAGATTTGACATGAGT+TGG - chr4.4:10265632-10265651 None:intergenic 40.0%
GTGAAAACAGAGTAGAGTGA+TGG - chr4.4:10265693-10265712 None:intergenic 40.0%
GTGACTCTAGCAAAAATTCG+AGG - chr4.4:10267133-10267152 None:intergenic 40.0%
GTGGATACATTCACATTCCA+AGG + chr4.4:10265528-10265547 MS.gene28097:CDS 40.0%
TATGGATTTCCTTCAGCGTT+TGG + chr4.4:10267880-10267899 MS.gene28097:CDS 40.0%
TGGAATGTGAATGTATCCAC+CGG - chr4.4:10265528-10265547 None:intergenic 40.0%
TGGTACTTTGTCACCAATTG+TGG + chr4.4:10265943-10265962 MS.gene28097:CDS 40.0%
! CTTCGGGTACTCTTTTAACA+CGG - chr4.4:10266882-10266901 None:intergenic 40.0%
! TACTGCTCATGGTGATTTGT+TGG + chr4.4:10267275-10267294 MS.gene28097:intron 40.0%
!! TACACAGAGATGGGTTGAAT+TGG + chr4.4:10266841-10266860 MS.gene28097:intron 40.0%
AAACTAATCCCACGAGGTGT+AGG - chr4.4:10267350-10267369 None:intergenic 45.0%
AGCTTCGTCTAACACAAGGA+AGG + chr4.4:10266900-10266919 MS.gene28097:CDS 45.0%
AGTACCGCTACCATTCATTC+CGG - chr4.4:10265474-10265493 None:intergenic 45.0%
ATCTCCAAGATCACGCTCTA+GGG + chr4.4:10267919-10267938 MS.gene28097:CDS 45.0%
ATGGGAACAAATTCTCTGGC+AGG - chr4.4:10266523-10266542 None:intergenic 45.0%
CCGATTCAGACATGAAAGCA+CGG - chr4.4:10265979-10265998 None:intergenic 45.0%
CCGTGCTTTCATGTCTGAAT+CGG + chr4.4:10265976-10265995 MS.gene28097:CDS 45.0%
CCTTGTGTTAGACGAAGCTT+CGG - chr4.4:10266899-10266918 None:intergenic 45.0%
CTAACACAAGGAAGGAAGGT+GGG + chr4.4:10266908-10266927 MS.gene28097:intron 45.0%
CTTGTGTTAGACGAAGCTTC+GGG - chr4.4:10266898-10266917 None:intergenic 45.0%
GAACTTGTGGTTCTCAACTC+TGG - chr4.4:10266704-10266723 None:intergenic 45.0%
GAGACCAAAACCGGAATGAA+TGG + chr4.4:10265461-10265480 MS.gene28097:CDS 45.0%
GGAATGTGAATGTATCCACC+GGG - chr4.4:10265527-10265546 None:intergenic 45.0%
TCGTCTAACACAAGGAAGGA+AGG + chr4.4:10266904-10266923 MS.gene28097:CDS 45.0%
TCTAACACAAGGAAGGAAGG+TGG + chr4.4:10266907-10266926 MS.gene28097:intron 45.0%
TTATGCTGGAAGTCATGGCA+TGG + chr4.4:10266293-10266312 MS.gene28097:CDS 45.0%
! GCTACCATTCATTCCGGTTT+TGG - chr4.4:10265468-10265487 None:intergenic 45.0%
! GTTGTGTCTGAGACCAAAAC+CGG + chr4.4:10265452-10265471 MS.gene28097:CDS 45.0%
! TTCAGACATGAAAGCACGGT+CGG - chr4.4:10265975-10265994 None:intergenic 45.0%
!!! TTTTTTTTTTTTTTATGTTT+TGG + chr4.4:10267170-10267189 MS.gene28097:intron 5.0%
CATCTCCAAGATCACGCTCT+AGG + chr4.4:10267918-10267937 MS.gene28097:CDS 50.0%
CCGAAGCTTCGTCTAACACA+AGG + chr4.4:10266896-10266915 MS.gene28097:CDS 50.0%
GGATTTCCTTCAGCGTTTGG+TGG + chr4.4:10267883-10267902 MS.gene28097:CDS 50.0%
TACACCCTAGAGCGTGATCT+TGG - chr4.4:10267926-10267945 None:intergenic 50.0%
TATGCAGCAGCTGTGTCCTT+TGG - chr4.4:10267749-10267768 None:intergenic 50.0%
! GAACAGAGGTGCAGAACTTG+TGG - chr4.4:10266717-10266736 None:intergenic 50.0%
! TCAGACATGAAAGCACGGTC+GGG - chr4.4:10265974-10265993 None:intergenic 50.0%
!! TGGTAGCGGTACTATTACCG+TGG + chr4.4:10265481-10265500 MS.gene28097:CDS 50.0%
CAAGGAAGGAAGGTGGGTCA+TGG + chr4.4:10266914-10266933 MS.gene28097:intron 55.0%
CGAACAGCTTCGCCATCCAT+CGG + chr4.4:10265850-10265869 MS.gene28097:intron 55.0%
TCCACTCCACCAAACGCTGA+AGG - chr4.4:10267892-10267911 None:intergenic 55.0%
TGGTTCTCAACTCTGGCACC+AGG - chr4.4:10266697-10266716 None:intergenic 55.0%
! CAGACATGAAAGCACGGTCG+GGG - chr4.4:10265973-10265992 None:intergenic 55.0%
! TCCTTCAGCGTTTGGTGGAG+TGG + chr4.4:10267888-10267907 MS.gene28097:CDS 55.0%
AGGGTGACACCTACACCTCG+TGG + chr4.4:10267338-10267357 MS.gene28097:intron 60.0%
CGGTCGGGGTCATCCACAAT+TGG - chr4.4:10265959-10265978 None:intergenic 60.0%
GGGTGACACCTACACCTCGT+GGG + chr4.4:10267339-10267358 MS.gene28097:intron 60.0%
TACCGTGGCACAGAAGCCAC+CGG + chr4.4:10265496-10265515 MS.gene28097:CDS 60.0%
! ATGTATCCACCGGGTGCTGC+CGG - chr4.4:10265518-10265537 None:intergenic 60.0%
TGCCGGTGGCTTCTGTGCCA+CGG - chr4.4:10265501-10265520 None:intergenic 65.0%
CAGAAGCCACCGGCAGCACC+CGG + chr4.4:10265506-10265525 MS.gene28097:CDS 70.0%
! TATCCACCGGGTGCTGCCGG+TGG - chr4.4:10265515-10265534 None:intergenic 70.0%
AAGCCACCGGCAGCACCCGG+TGG + chr4.4:10265509-10265528 MS.gene28097:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 10265434 10267946 10265434 ID=MS.gene28097
chr4.4 mRNA 10265434 10267946 10265434 ID=MS.gene28097.t1;Parent=MS.gene28097
chr4.4 exon 10265434 10265682 10265434 ID=MS.gene28097.t1.exon1;Parent=MS.gene28097.t1
chr4.4 CDS 10265434 10265682 10265434 ID=cds.MS.gene28097.t1;Parent=MS.gene28097.t1
chr4.4 exon 10265857 10265997 10265857 ID=MS.gene28097.t1.exon2;Parent=MS.gene28097.t1
chr4.4 CDS 10265857 10265997 10265857 ID=cds.MS.gene28097.t1;Parent=MS.gene28097.t1
chr4.4 exon 10266091 10266162 10266091 ID=MS.gene28097.t1.exon3;Parent=MS.gene28097.t1
chr4.4 CDS 10266091 10266162 10266091 ID=cds.MS.gene28097.t1;Parent=MS.gene28097.t1
chr4.4 exon 10266261 10266353 10266261 ID=MS.gene28097.t1.exon4;Parent=MS.gene28097.t1
chr4.4 CDS 10266261 10266353 10266261 ID=cds.MS.gene28097.t1;Parent=MS.gene28097.t1
chr4.4 exon 10266490 10266552 10266490 ID=MS.gene28097.t1.exon5;Parent=MS.gene28097.t1
chr4.4 CDS 10266490 10266552 10266490 ID=cds.MS.gene28097.t1;Parent=MS.gene28097.t1
chr4.4 exon 10266658 10266759 10266658 ID=MS.gene28097.t1.exon6;Parent=MS.gene28097.t1
chr4.4 CDS 10266658 10266759 10266658 ID=cds.MS.gene28097.t1;Parent=MS.gene28097.t1
chr4.4 exon 10266848 10266925 10266848 ID=MS.gene28097.t1.exon7;Parent=MS.gene28097.t1
chr4.4 CDS 10266848 10266925 10266848 ID=cds.MS.gene28097.t1;Parent=MS.gene28097.t1
chr4.4 exon 10267086 10267155 10267086 ID=MS.gene28097.t1.exon8;Parent=MS.gene28097.t1
chr4.4 CDS 10267086 10267155 10267086 ID=cds.MS.gene28097.t1;Parent=MS.gene28097.t1
chr4.4 exon 10267470 10267540 10267470 ID=MS.gene28097.t1.exon9;Parent=MS.gene28097.t1
chr4.4 CDS 10267470 10267540 10267470 ID=cds.MS.gene28097.t1;Parent=MS.gene28097.t1
chr4.4 exon 10267698 10267790 10267698 ID=MS.gene28097.t1.exon10;Parent=MS.gene28097.t1
chr4.4 CDS 10267698 10267790 10267698 ID=cds.MS.gene28097.t1;Parent=MS.gene28097.t1
chr4.4 exon 10267878 10267946 10267878 ID=MS.gene28097.t1.exon11;Parent=MS.gene28097.t1
chr4.4 CDS 10267878 10267946 10267878 ID=cds.MS.gene28097.t1;Parent=MS.gene28097.t1
Gene Sequence

>MS.gene28097

ATGACTCAAAAGAATGTGGTTGTGTCTGAGACCAAAACCGGAATGAATGGTAGCGGTACTATTACCGTGGCACAGAAGCCACCGGCAGCACCCGGTGGATACATTCACATTCCAAGGAGGAGAATTTTGAAGAATCTTGAAATCAATGGAGGACAAAGAATCAATACATGGATTGATTCTATGAGAGCTTCTTCTCCAACTCATGTCAAATCTTCTCCTTCTTTAGCTGAAGAATACAATTCTTGGATTGTGAGCACCATCACTCTACTCTGTTTTCACTGTTATATATATATATATATATTTATTTCAATGAAACCACCAAAATTTTGAACATATACTGGATCTGACTTTCTTTATCATAGTAAAATACTCGTGCATTCACACATCGCCTTATTATTTATACGTGTTGTTTTTATCGAACAGCTTCGCCATCCATCGGCATTAGATATGTTTGAACAAATTATGGATGCTGCAAAGGGAAAACAGATTGTTATGTTTTTGGATTATGATGGTACTTTGTCACCAATTGTGGATGACCCCGACCGTGCTTTCATGTCTGAATCGGTTAGTTTTTGTTTATAAAACTGGTTTATATATCCTTTAAATAAATCTGTTAATTTAGTTTTCTAATAAACTGTATTATCACATATATATCAGATGAGGAAAACTGTTAAGAAACTTGCAAGATGTTTTCCTACTGCAATTGTTACTGGAAGATGCATAGACAAAGTACACTTTCACTCTTTCAATTTCTATTCTACCCTTTTTTTATCTTTTTTGAAATGAAAATAAATTGATTATTATTTTTTTCTGCAAACAATTTTCAGGTGTACAATTTTGTTCGTTTGGATGAACTATATTATGCTGGAAGTCATGGCATGGATATTAAAGGGCCAACAAAAGAGTCCAAATACAACAAAGTGAGTAAACTAGCTCTAGTGTATTTATTGTACCACAGTACAGTATATTTTTTAATTTGACCTATATAGTGCCGTGGAATAAGGTCTTAAAATTTTTAAATTCTAAAAATTAATTAACATTGGTAATGGTTTGTAGAATAACAAAGCAGAGGAAGTACTTTTTCAACCTGCCAGAGAATTTGTTCCCATGATAAATGAGGTTAGTCACACTCACAACAAAACTCTTCATGAAATTTCAATTTTTTTTTTTCAAAAAAAATTATAATATGCAAAACTGATTTATTTTACCTTTGTTAATTTTCAGGTGTACGAACAACTAGTTGAGAAAACAAAATCAACTCCTGGTGCCAGAGTTGAGAACCACAAGTTCTGCACCTCTGTTCACTTTAGATGTGTTGATGAAAAGGTATATTATTAGCAGTTTATAGCAATTAATCAGTGTTATTGATGCTATAATATTTATGATCTAACTCAAAGTTGTTTATTGTACACAGAGATGGGTTGAATTGGCACAGCAAGTAAAATCCGTGTTAAAAGAGTACCCGAAGCTTCGTCTAACACAAGGAAGGAAGGTGGGTCATGGTTCTATTTTCTTTATAATCAACTTTTTTGGAGGGAGTGTATATTTTTAATGGTGGGGGTGTGATTTAATTAGTTCAAAGGTGATTATGACTGGGATTAAGAATAAGAAATAAGAGATGTCTCACTTTCAATGTCAATTGTTTAATACAGGTATTGGAGATTCGTCCTGCAATTAAATGGGACAAAGGCAAGGCCCTCGAATTTTTGCTAGAGTCACTTGGTAAGTTTTAATTTTTTTTTTTTTTTTTATGTTTTGGTTCTTCATTTGTCACAATCAAACATATATGTAATACATATATTTCATAGAACATATAATAATACACAAACTTATAAATATGCTACTGCTCATGGTGATTTGTTGGATAATTATTCCAAGTATTTGACCATTATGACAGAATTAAGAGGGTGACACCTACACCTCGTGGGATTAGTTTACTCATAAGATTACTGTATTTTATATCCATGCGTTTCTAAATATATATTCATATTTTAATTTGTCCATCAATTTTCTAACTAATAAATCGTTTTCAACAGGATTTGCCAACTGTAACGATGTATTTCCTGTTTATATTGGTGATGATAGAACCGATGAAGATGCATTCAAGGTATCCAATGATCTTAATTGTTTTTTTCCTCCAATTTTTTATTTCTAAATGAACAGTGTTTAATTAATTAGTACCAAGTCAATTATGTTTAATTCTTTGTAGACTGTTTTGGATAATTGAACTAACTAGTTGTTTTAAATTGTTTAATCATAAACAGAAATTAAGAGACATAGATCAAGGTTTTGGAATTCTAGTCTCTAAGTTTCCAAAGGACACAGCTGCTGCATACTCTTTACAAGAGCCTAATGAGGTAAGCATAATTTCATCTATTTCTTGATTATTGTATCATATGCCTTAAATATATGTTTTACTGATTTATTAATTTAATATCATTCAGGTTATGGATTTCCTTCAGCGTTTGGTGGAGTGGAAAAAAACATCTCCAAGATCACGCTCTAGGGTGTAA
Protein sequence

>MS.gene28097.t1

MTQKNVVVSETKTGMNGSGTITVAQKPPAAPGGYIHIPRRRILKNLEINGGQRINTWIDSMRASSPTHVKSSPSLAEEYNSWILRHPSALDMFEQIMDAAKGKQIVMFLDYDGTLSPIVDDPDRAFMSESMRKTVKKLARCFPTAIVTGRCIDKVYNFVRLDELYYAGSHGMDIKGPTKESKYNKNNKAEEVLFQPAREFVPMINEVYEQLVEKTKSTPGARVENHKFCTSVHFRCVDEKRWVELAQQVKSVLKEYPKLRLTQGRKVLEIRPAIKWDKGKALEFLLESLGFANCNDVFPVYIGDDRTDEDAFKKLRDIDQGFGILVSKFPKDTAAAYSLQEPNEVMDFLQRLVEWKKTSPRSRSRV