AlfalfaGEDB | MS.gene28133

M. sativa cultivar XinJiangDaYe / MS.gene28133

KEGG: K08065
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0046982 GO:0046983 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0110165 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00808.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28133.t1	RHN42986.1	94.1	203	5	1	1	196	1	203	2.90E-101	377.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28133.t1	O23310	85.6	104	15	0	23	126	16	119	2.5e-45	183.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28133.t1	A0A396GPD3	94.1	203	5	1	1	196	1	203	2.1e-101	377.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene28133.t1	TF	NF-YB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene059713	MS.gene28133	0.817235	3.66E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28133.t1	MTR_8g091720	93.717	191	5	1	1	184	1	191	9.77e-131	365
MS.gene28133.t1	MTR_5g095740	79.487	117	22	1	13	129	10	124	8.07e-63	192
MS.gene28133.t1	MTR_2g026710	80.702	114	20	1	24	137	13	124	2.57e-62	191
MS.gene28133.t1	MTR_3g058980	87.879	99	12	0	25	123	24	122	4.04e-60	186
MS.gene28133.t1	MTR_7g061270	75.439	114	28	0	13	126	11	124	1.31e-59	184
MS.gene28133.t1	MTR_7g100650	75.439	114	28	0	13	126	11	124	1.34e-59	184
MS.gene28133.t1	MTR_8g093920	76.636	107	25	0	27	133	39	145	1.44e-56	178
MS.gene28133.t1	MTR_1g072790	76.699	103	24	0	23	125	24	126	2.27e-56	176
MS.gene28133.t1	MTR_4g133938	74.312	109	28	0	17	125	8	116	2.17e-53	167
MS.gene28133.t1	MTR_1g088860	69.307	101	31	0	22	122	30	130	1.83e-48	157
MS.gene28133.t1	MTR_0392s0020	64.151	106	38	0	21	126	11	116	2.69e-48	154
MS.gene28133.t1	MTR_4g119500	56.557	122	53	0	12	133	6	127	2.47e-47	151
MS.gene28133.t1	MTR_4g133952	64.948	97	34	0	27	123	57	153	4.84e-46	152
MS.gene28133.t1	MTR_1g039040	62.745	102	38	0	27	128	5	106	1.38e-44	146
MS.gene28133.t1	MTR_1g083070	61.957	92	35	0	28	119	4	95	7.69e-39	130
MS.gene28133.t1	MTR_1g029070	49.580	119	54	1	14	126	32	150	2.45e-32	115
MS.gene28133.t1	MTR_5g095900	44.330	97	51	1	24	117	9	105	1.50e-21	87.4
MS.gene28133.t1	MTR_1g028480	53.125	64	30	0	58	121	19	82	2.13e-18	77.0
MS.gene28133.t1	MTR_1g029100	40.789	76	45	0	33	108	7	82	1.91e-17	73.9
MS.gene28133.t1	MTR_2g056000	32.143	84	57	0	22	105	35	118	1.20e-13	66.6
MS.gene28133.t1	MTR_4g112380	33.766	77	51	0	29	105	12	88	2.63e-13	65.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28133.t1	AT4G14540	81.818	110	20	0	24	133	17	126	3.65e-64	195
MS.gene28133.t1	AT5G47640	85.859	99	14	0	25	123	24	122	6.47e-60	186
MS.gene28133.t1	AT2G13570	74.576	118	29	1	8	125	17	133	1.92e-59	185
MS.gene28133.t1	AT3G53340	68.800	125	28	2	14	133	21	139	1.87e-56	176
MS.gene28133.t1	AT3G53340	68.800	125	28	2	14	133	21	139	1.87e-56	176
MS.gene28133.t1	AT2G37060	71.552	116	28	1	27	137	29	144	4.54e-56	175
MS.gene28133.t1	AT2G37060	71.552	116	28	1	27	137	29	144	4.54e-56	175
MS.gene28133.t1	AT2G37060	71.552	116	28	1	27	137	29	144	4.54e-56	175
MS.gene28133.t1	AT2G38880	69.643	112	34	0	14	125	7	118	7.77e-55	171
MS.gene28133.t1	AT3G53340	75.238	105	20	1	14	118	21	119	9.01e-55	170
MS.gene28133.t1	AT2G38880	69.643	112	34	0	14	125	7	118	1.20e-54	171
MS.gene28133.t1	AT2G38880	69.643	112	34	0	14	125	7	118	1.20e-54	171
MS.gene28133.t1	AT2G38880	69.643	112	34	0	14	125	7	118	1.20e-54	171
MS.gene28133.t1	AT3G53340	75.472	106	20	1	14	119	21	120	2.67e-54	170
MS.gene28133.t1	AT3G53340	75.472	106	20	1	14	119	21	120	2.67e-54	170
MS.gene28133.t1	AT2G38880	71.429	105	30	0	14	118	7	111	1.13e-52	164
MS.gene28133.t1	AT2G38880	67.544	114	37	0	14	127	7	120	1.39e-52	165
MS.gene28133.t1	AT2G38880	71.429	105	30	0	14	118	7	111	3.32e-52	165
MS.gene28133.t1	AT2G38880	71.429	105	30	0	14	118	7	111	3.32e-52	165
MS.gene28133.t1	AT2G38880	77.660	94	21	0	25	118	18	111	5.34e-52	164
MS.gene28133.t1	AT2G38880	77.660	94	21	0	25	118	18	111	5.34e-52	164
MS.gene28133.t1	AT2G38880	77.660	94	21	0	25	118	18	111	5.34e-52	164
MS.gene28133.t1	AT2G47810	71.845	103	29	0	22	124	45	147	3.72e-51	162
MS.gene28133.t1	AT5G47670	60.714	112	44	0	16	127	17	128	6.05e-48	155
MS.gene28133.t1	AT5G47670	60.714	112	44	0	16	127	46	157	1.49e-47	155
MS.gene28133.t1	AT5G47670	60.714	112	44	0	16	127	46	157	1.49e-47	155
MS.gene28133.t1	AT1G21970	62.000	100	38	0	26	125	57	156	2.48e-45	150
MS.gene28133.t1	AT1G09030	62.500	96	36	0	28	123	3	98	1.22e-42	140
MS.gene28133.t1	AT5G08190	35.897	78	50	0	28	105	11	88	1.70e-14	68.2
MS.gene28133.t1	AT5G08190	35.897	78	50	0	28	105	11	88	1.83e-14	68.2
MS.gene28133.t1	AT5G23090	33.766	77	51	0	29	105	12	88	4.99e-14	67.0
MS.gene28133.t1	AT5G23090	33.766	77	51	0	29	105	12	88	4.99e-14	67.0
MS.gene28133.t1	AT5G23090	33.766	77	51	0	29	105	12	88	4.99e-14	67.0
MS.gene28133.t1	AT5G23090	33.766	77	51	0	29	105	12	88	4.99e-14	67.0

Find 40 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTAATGGTGATGATCTTCTT+TGG	0.277181	4.4:-9874275	MS.gene28133:CDS
TTAATCTCTTGGAACCTCTT+TGG	0.292077	4.4:+9874078	None:intergenic
AAGTTTATCTTAATAATTAT+AGG	0.297968	4.4:-9874209	MS.gene28133:CDS
GAGTTCATAAGTTTCATTAC+TGG	0.304041	4.4:-9874339	MS.gene28133:CDS
TGCCACCAAACCTACGTTAC+AGG	0.318400	4.4:-9873978	MS.gene28133:CDS
GAAAAGAGGAAAACAATTAA+TGG	0.329176	4.4:-9874291	MS.gene28133:CDS
AGTTTATCTTAATAATTATA+GGG	0.334719	4.4:-9874208	MS.gene28133:CDS
ATTGTGGATTATAGAGTAAT+TGG	0.335947	4.4:-9874048	MS.gene28133:CDS
AAGAGGTTCCAAGAGATTAA+TGG	0.350652	4.4:-9874075	MS.gene28133:CDS
TAATGGTGATGATCTTCTTT+GGG	0.364795	4.4:-9874274	MS.gene28133:CDS
GACTTCCTACAGGACTTGTT+TGG	0.366205	4.4:+9874514	None:intergenic
ATTATTAATTTCAACATGTC+AGG	0.404942	4.4:-9874549	None:intergenic
CAATCCCTCCATTAATCTCT+TGG	0.406112	4.4:+9874067	None:intergenic
GATGAAACAGAACATGCAAT+TGG	0.429214	4.4:-9874018	MS.gene28133:CDS
CCTGATGTTGGACTTCCTAC+AGG	0.434628	4.4:+9874504	None:intergenic
TCTGAGATGTTTCCTGATGT+TGG	0.453105	4.4:+9874492	None:intergenic
ACGTGAGCCGTATAATGAAA+AGG	0.454500	4.4:-9874425	MS.gene28133:CDS
ATACGGCTCACGTTAGCAAT+TGG	0.458481	4.4:+9874435	None:intergenic
CTTTGGGCTATGACAACACT+TGG	0.464387	4.4:-9874258	MS.gene28133:CDS
AATAATTATAGGGAAATTGA+AGG	0.468083	4.4:-9874198	MS.gene28133:CDS
TCAACCCTGTAACGTAGGTT+TGG	0.472415	4.4:+9873973	None:intergenic
TCAATTGATGTTAATGATGT+TGG	0.481755	4.4:-9874117	MS.gene28133:CDS
CCTGTAGGAAGTCCAACATC+AGG	0.498605	4.4:-9874504	MS.gene28133:CDS
ACCTACGTTACAGGGTTGAA+TGG	0.502904	4.4:-9873969	MS.gene28133:CDS
AGAGATTAATGGAGGGATTG+TGG	0.513734	4.4:-9874064	MS.gene28133:CDS
CGTGAGCCGTATAATGAAAA+GGG	0.518250	4.4:-9874424	MS.gene28133:CDS
ACAGAACATGCAATTGGAAG+TGG	0.518322	4.4:-9874012	MS.gene28133:CDS
ATTGATGTTAATGATGTTGG+TGG	0.525414	4.4:-9874114	MS.gene28133:CDS
ACCCTGTAACGTAGGTTTGG+TGG	0.528618	4.4:+9873976	None:intergenic
GATGTTAATGATGTTGGTGG+TGG	0.530464	4.4:-9874111	MS.gene28133:CDS
CATCATCAAAAGAACAAGAC+AGG	0.530857	4.4:-9874464	MS.gene28133:CDS
ATAAGTGCCAGAGAGAAAAG+AGG	0.537335	4.4:-9874305	MS.gene28133:CDS
CTCTAAAGAAGCAAAAGAAA+CGG	0.538600	4.4:-9874379	MS.gene28133:CDS
TTATTAAGATAAACTTTCAA+AGG	0.539135	4.4:+9874216	None:intergenic
GCCACCAAACCTACGTTACA+GGG	0.557356	4.4:-9873977	MS.gene28133:CDS
AGAAACCAAACAAGTCCTGT+AGG	0.592028	4.4:-9874519	MS.gene28133:CDS
ACCATTCAACCCTGTAACGT+AGG	0.617328	4.4:+9873968	None:intergenic
AGGTTCCAAGAGATTAATGG+AGG	0.632716	4.4:-9874072	MS.gene28133:CDS
GGCTCACGTTAGCAATTGGT+AGG	0.662678	4.4:+9874439	None:intergenic
GGTTCCAAGAGATTAATGGA+GGG	0.665223	4.4:-9874071	MS.gene28133:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGTTTATCTTAATAATTAT+AGG	-	chr4.4:9874291-9874310	MS.gene28133:CDS	10.0%
!!	AGTTTATCTTAATAATTATA+GGG	-	chr4.4:9874292-9874311	MS.gene28133:CDS	10.0%
!!	TTATTAAGATAAACTTTCAA+AGG	+	chr4.4:9874287-9874306	None:intergenic	15.0%
!!	AATAATTATAGGGAAATTGA+AGG	-	chr4.4:9874302-9874321	MS.gene28133:CDS	20.0%
!	GAAAAGAGGAAAACAATTAA+TGG	-	chr4.4:9874209-9874228	MS.gene28133:CDS	25.0%
!	TCAATTGATGTTAATGATGT+TGG	-	chr4.4:9874383-9874402	MS.gene28133:CDS	25.0%
!!	ATTGTGGATTATAGAGTAAT+TGG	-	chr4.4:9874452-9874471	MS.gene28133:CDS	25.0%
	ATTGATGTTAATGATGTTGG+TGG	-	chr4.4:9874386-9874405	MS.gene28133:CDS	30.0%
	GAGTTCATAAGTTTCATTAC+TGG	-	chr4.4:9874161-9874180	MS.gene28133:CDS	30.0%
!	CTCTAAAGAAGCAAAAGAAA+CGG	-	chr4.4:9874121-9874140	MS.gene28133:CDS	30.0%
!	TAATGGTGATGATCTTCTTT+GGG	-	chr4.4:9874226-9874245	MS.gene28133:CDS	30.0%
!	TTAATGGTGATGATCTTCTT+TGG	-	chr4.4:9874225-9874244	MS.gene28133:CDS	30.0%
!!	TTAGAGATTTTAGCATTTGC+TGG	+	chr4.4:9874107-9874126	None:intergenic	30.0%
!!!	CTTGGTTTTGAAAACTATGT+TGG	-	chr4.4:9874260-9874279	MS.gene28133:CDS	30.0%
	AAGAGGTTCCAAGAGATTAA+TGG	-	chr4.4:9874425-9874444	MS.gene28133:CDS	35.0%
	CATCATCAAAAGAACAAGAC+AGG	-	chr4.4:9874036-9874055	MS.gene28133:CDS	35.0%
	GATGAAACAGAACATGCAAT+TGG	-	chr4.4:9874482-9874501	MS.gene28133:CDS	35.0%
	TTAATCTCTTGGAACCTCTT+TGG	+	chr4.4:9874425-9874444	None:intergenic	35.0%
!	TTGTTTTCCTCTTTTCTCTC+TGG	+	chr4.4:9874205-9874224	None:intergenic	35.0%
!!	GAAGTGCCCTTTTCATTATA+CGG	+	chr4.4:9874085-9874104	None:intergenic	35.0%
	ACAGAACATGCAATTGGAAG+TGG	-	chr4.4:9874488-9874507	MS.gene28133:CDS	40.0%
	ACGTGAGCCGTATAATGAAA+AGG	-	chr4.4:9874075-9874094	MS.gene28133:CDS	40.0%
	AGAAACCAAACAAGTCCTGT+AGG	-	chr4.4:9873981-9874000	MS.gene28133:CDS	40.0%
	AGAGATTAATGGAGGGATTG+TGG	-	chr4.4:9874436-9874455	MS.gene28133:CDS	40.0%
	AGGTTCCAAGAGATTAATGG+AGG	-	chr4.4:9874428-9874447	MS.gene28133:CDS	40.0%
	ATAAGTGCCAGAGAGAAAAG+AGG	-	chr4.4:9874195-9874214	MS.gene28133:CDS	40.0%
	CAATCCCTCCATTAATCTCT+TGG	+	chr4.4:9874436-9874455	None:intergenic	40.0%
	CGTGAGCCGTATAATGAAAA+GGG	-	chr4.4:9874076-9874095	MS.gene28133:CDS	40.0%
	GGTTCCAAGAGATTAATGGA+GGG	-	chr4.4:9874429-9874448	MS.gene28133:CDS	40.0%
!	GATGTTAATGATGTTGGTGG+TGG	-	chr4.4:9874389-9874408	MS.gene28133:CDS	40.0%
!	GTGGATTTTATGCTCCAAAG+AGG	-	chr4.4:9874408-9874427	MS.gene28133:CDS	40.0%
!	TCTGAGATGTTTCCTGATGT+TGG	+	chr4.4:9874011-9874030	None:intergenic	40.0%
	ACCATTCAACCCTGTAACGT+AGG	+	chr4.4:9874535-9874554	None:intergenic	45.0%
	ACCTACGTTACAGGGTTGAA+TGG	-	chr4.4:9874531-9874550	MS.gene28133:CDS	45.0%
	ATACGGCTCACGTTAGCAAT+TGG	+	chr4.4:9874068-9874087	None:intergenic	45.0%
	CTTTGGGCTATGACAACACT+TGG	-	chr4.4:9874242-9874261	MS.gene28133:CDS	45.0%
	TCAACCCTGTAACGTAGGTT+TGG	+	chr4.4:9874530-9874549	None:intergenic	45.0%
!	GACTTCCTACAGGACTTGTT+TGG	+	chr4.4:9873989-9874008	None:intergenic	45.0%
	ACCCTGTAACGTAGGTTTGG+TGG	+	chr4.4:9874527-9874546	None:intergenic	50.0%
	CCTGATGTTGGACTTCCTAC+AGG	+	chr4.4:9873999-9874018	None:intergenic	50.0%
	CCTGTAGGAAGTCCAACATC+AGG	-	chr4.4:9873996-9874015	MS.gene28133:CDS	50.0%
	GCCACCAAACCTACGTTACA+GGG	-	chr4.4:9874523-9874542	MS.gene28133:CDS	50.0%
	GGCTCACGTTAGCAATTGGT+AGG	+	chr4.4:9874064-9874083	None:intergenic	50.0%
	TGCCACCAAACCTACGTTAC+AGG	-	chr4.4:9874522-9874541	MS.gene28133:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	9873966	9874556	9873966	ID=MS.gene28133
chr4.4	mRNA	9873966	9874556	9873966	ID=MS.gene28133.t1;Parent=MS.gene28133
chr4.4	exon	9873966	9874556	9873966	ID=MS.gene28133.t1.exon1;Parent=MS.gene28133.t1
chr4.4	CDS	9873966	9874556	9873966	ID=cds.MS.gene28133.t1;Parent=MS.gene28133.t1

Gene Sequence

>MS.gene28133

ATGTCAGGTAATAAGAGAAACCAAACAAGTCCTGTAGGAAGTCCAACATCAGGAAACATCTCAGACAGTTCATCATCAAAAGAACAAGACAGGTTCCTACCAATTGCTAACGTGAGCCGTATAATGAAAAGGGCACTTCCAGCAAATGCTAAAATCTCTAAAGAAGCAAAAGAAACGGTTCAAGAATGTGTCTCTGAGTTCATAAGTTTCATTACTGGTGAAGCTTCTGATAAGTGCCAGAGAGAAAAGAGGAAAACAATTAATGGTGATGATCTTCTTTGGGCTATGACAACACTTGGTTTTGAAAACTATGTTGGTCCTTTGAAAGTTTATCTTAATAATTATAGGGAAATTGAAGGAGAAAAGAGTAATTCTAGTGCTACTAAACAAGATGATCAATATGATCATAATTCTTCTTCAATTGATGTTAATGATGTTGGTGGTGGATTTTATGCTCCAAAGAGGTTCCAAGAGATTAATGGAGGGATTGTGGATTATAGAGTAATTGGTAATAGTGATGAAACAGAACATGCAATTGGAAGTGGTAATAGAATCATGCCACCAAACCTACGTTACAGGGTTGAATGGTAG

Protein sequence

>MS.gene28133.t1

MSGNKRNQTSPVGSPTSGNISDSSSSKEQDRFLPIANVSRIMKRALPANAKISKEAKETVQECVSEFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFENYVGPLKVYLNNYREIEGEKSNSSATKQDDQYDHNSSSIDVNDVGGGFYAPKRFQEINGGIVDYRVIGNSDETEHAIGSGNRIMPPNLRYRVEW