AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28552

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28552.t1 MTR_8g079250 96.597 382 13 0 1 382 613 994 0.0 744
MS.gene28552.t1 MTR_5g010420 79.319 382 77 2 1 382 606 985 0.0 622
MS.gene28552.t1 MTR_8g079250 95.902 244 10 0 1 244 613 856 2.47e-151 449
MS.gene28552.t1 MTR_8g012390 61.905 378 137 2 1 374 583 957 6.81e-146 437
MS.gene28552.t1 MTR_4g094695 56.154 390 157 3 1 376 600 989 1.54e-135 411
MS.gene28552.t1 MTR_2g035840 56.764 377 154 1 1 368 604 980 1.25e-134 409
MS.gene28552.t1 MTR_4g094232 41.161 379 197 5 1 363 539 907 3.46e-79 261
MS.gene28552.t1 MTR_8g012390 54.435 248 106 2 1 244 583 827 3.18e-77 254
MS.gene28552.t1 MTR_3g105190 37.662 385 217 6 1 369 509 886 2.87e-69 234
MS.gene28552.t1 MTR_4g127580 34.133 375 210 11 1 362 347 697 5.12e-47 172
MS.gene28552.t1 MTR_2g036380 29.659 381 227 11 1 366 159 513 1.68e-45 166
MS.gene28552.t1 MTR_2g036380 29.659 381 227 11 1 366 190 544 2.40e-45 166
MS.gene28552.t1 MTR_2g036380 29.973 377 223 11 5 366 256 606 3.37e-45 166
MS.gene28552.t1 MTR_2g036380 29.973 377 223 11 5 366 346 696 8.12e-45 165
MS.gene28552.t1 MTR_8g079250 94.805 77 4 0 1 77 613 689 2.60e-40 152
MS.gene28552.t1 MTR_3g105190 32.959 267 158 5 1 253 509 768 2.47e-32 129
MS.gene28552.t1 MTR_4g094985 30.239 377 212 14 1 343 399 758 4.11e-29 119
MS.gene28552.t1 MTR_5g015590 33.898 177 88 3 177 324 649 825 4.69e-22 98.6
MS.gene28552.t1 MTR_5g015590 33.898 177 88 3 177 324 649 825 6.24e-22 98.2
MS.gene28552.t1 MTR_5g015590 33.898 177 88 3 177 324 649 825 6.27e-22 98.2
MS.gene28552.t1 MTR_3g103070 32.955 176 90 3 177 324 476 651 5.53e-21 95.5
MS.gene28552.t1 MTR_3g103070 32.955 176 90 3 177 324 652 827 5.57e-21 95.5
MS.gene28552.t1 MTR_4g096990 32.768 177 90 3 177 324 647 823 9.46e-21 94.7
MS.gene28552.t1 MTR_2g036650 26.617 402 214 10 11 343 294 683 1.58e-19 90.9
MS.gene28552.t1 MTR_1g009720 25.945 397 194 10 11 332 289 660 3.52e-19 90.1
MS.gene28552.t1 MTR_0005s0140 32.768 177 91 3 176 324 568 744 5.09e-19 89.4
MS.gene28552.t1 MTR_4g043690 32.768 177 91 3 176 324 653 829 5.20e-19 89.7
MS.gene28552.t1 MTR_7g117500 25.750 400 218 13 11 343 296 683 1.12e-18 88.6
MS.gene28552.t1 MTR_8g090125 31.667 180 94 3 176 326 603 782 7.37e-18 85.9
MS.gene28552.t1 MTR_8g013780 29.508 183 97 3 176 326 193 375 8.29e-18 85.5
MS.gene28552.t1 MTR_3g108800 30.851 188 93 2 175 332 479 659 8.54e-18 85.9
MS.gene28552.t1 MTR_7g100110 27.004 237 117 4 122 321 597 814 8.67e-18 85.9
MS.gene28552.t1 MTR_3g108800 29.851 201 106 3 175 343 480 677 8.94e-18 85.9
MS.gene28552.t1 MTR_4g127710 29.646 226 116 4 175 360 483 705 8.94e-18 85.9
MS.gene28552.t1 MTR_2g038310 26.568 271 153 5 102 326 609 879 9.22e-18 85.9
MS.gene28552.t1 MTR_4g008170 27.358 212 116 4 150 326 691 899 9.52e-18 85.9
MS.gene28552.t1 MTR_4g127710 29.646 226 116 4 175 360 483 705 9.60e-18 85.5
MS.gene28552.t1 MTR_2g038310 26.568 271 153 5 102 326 609 879 9.64e-18 85.9
MS.gene28552.t1 MTR_7g117500 25.707 389 221 14 11 343 296 672 1.09e-17 85.5
MS.gene28552.t1 MTR_4g008170 27.358 212 116 4 150 326 684 892 1.10e-17 85.5
MS.gene28552.t1 MTR_8g063880 26.276 392 199 8 11 332 289 660 1.23e-17 85.5
MS.gene28552.t1 MTR_6g016470 29.032 186 100 3 173 326 638 823 1.52e-17 85.1
MS.gene28552.t1 MTR_4g116950 29.787 188 95 2 175 332 481 661 1.78e-17 84.7
MS.gene28552.t1 MTR_6g011310 29.353 201 107 3 175 343 477 674 1.90e-17 84.7
MS.gene28552.t1 MTR_1g009760 30.319 188 98 2 175 332 483 667 2.04e-17 84.7
MS.gene28552.t1 MTR_1g009760 30.319 188 94 2 175 332 480 660 2.22e-17 84.7
MS.gene28552.t1 MTR_6g011310 29.353 201 107 3 175 343 482 679 2.22e-17 84.7
MS.gene28552.t1 MTR_4g008650 31.461 178 93 3 176 324 645 822 2.29e-17 84.3
MS.gene28552.t1 MTR_4g008650 31.461 178 93 3 176 324 645 822 2.55e-17 84.3
MS.gene28552.t1 MTR_8g006790 26.396 394 223 11 11 343 297 684 3.57e-17 84.0
MS.gene28552.t1 MTR_7g095710 27.568 185 100 2 176 326 672 856 5.60e-17 83.2
MS.gene28552.t1 MTR_4g107500 28.358 201 109 3 175 343 484 681 6.08e-17 83.2
MS.gene28552.t1 MTR_2g105830 29.730 185 92 4 150 299 674 855 1.17e-16 82.4
MS.gene28552.t1 MTR_2g104860 29.947 187 93 2 176 324 479 665 1.37e-16 82.0
MS.gene28552.t1 MTR_5g015590 36.567 134 56 3 177 281 649 782 2.66e-16 81.3
MS.gene28552.t1 MTR_2g449840 29.545 176 94 1 176 321 482 657 4.99e-16 80.5
MS.gene28552.t1 MTR_8g027160 28.994 169 86 2 176 310 619 787 4.66e-15 77.4
MS.gene28552.t1 MTR_8g027160 28.994 169 86 2 176 310 619 787 5.53e-15 77.4
MS.gene28552.t1 MTR_3g103270 27.411 197 109 3 173 335 611 807 5.96e-15 77.0
MS.gene28552.t1 MTR_8g089870 30.625 160 85 2 173 306 515 674 1.03e-14 76.3
MS.gene28552.t1 MTR_8g446010 30.488 164 85 3 176 310 601 764 4.67e-14 74.3
MS.gene28552.t1 MTR_8g045070 30.488 164 85 3 176 310 578 741 5.03e-14 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28552.t1 AT1G63440 76.963 382 87 1 1 382 613 993 0.0 613
MS.gene28552.t1 AT5G44790 55.641 390 160 1 1 377 610 999 7.70e-136 412
MS.gene28552.t1 AT5G21930 36.829 391 223 7 1 374 477 860 2.58e-74 247
MS.gene28552.t1 AT5G21930 36.829 391 223 7 1 374 500 883 3.05e-74 247
MS.gene28552.t1 AT5G21930 36.829 391 223 7 1 374 500 883 3.05e-74 247
MS.gene28552.t1 AT4G33520 38.501 387 213 7 1 373 550 925 2.69e-66 226
MS.gene28552.t1 AT4G33520 38.501 387 213 7 1 373 550 925 3.78e-66 226
MS.gene28552.t1 AT5G21930 34.615 312 180 7 1 295 500 804 3.44e-49 177
MS.gene28552.t1 AT4G30110 31.915 376 231 9 1 369 343 700 1.86e-46 170
MS.gene28552.t1 AT4G30110 31.915 376 231 9 1 369 343 700 1.86e-46 170
MS.gene28552.t1 AT2G19110 30.380 395 240 12 1 378 353 729 8.93e-44 162
MS.gene28552.t1 AT2G19110 30.380 395 240 12 1 378 353 729 8.93e-44 162
MS.gene28552.t1 AT2G19110 30.380 395 240 12 1 378 353 729 8.93e-44 162
MS.gene28552.t1 AT4G37270 29.255 376 216 15 1 343 406 764 1.10e-23 103
MS.gene28552.t1 AT4G37640 32.768 177 90 3 177 324 649 825 1.18e-20 94.7
MS.gene28552.t1 AT1G27770 32.203 177 91 3 177 324 589 765 6.52e-20 92.4
MS.gene28552.t1 AT2G41560 32.402 179 93 2 176 326 643 821 6.58e-20 92.4
MS.gene28552.t1 AT1G27770 32.203 177 91 3 177 324 652 828 6.86e-20 92.4
MS.gene28552.t1 AT1G27770 32.203 177 91 3 177 324 652 828 6.86e-20 92.4
MS.gene28552.t1 AT2G41560 32.402 179 93 2 176 326 541 719 7.32e-20 92.0
MS.gene28552.t1 AT2G41560 32.402 179 93 2 176 326 541 719 7.32e-20 92.0
MS.gene28552.t1 AT2G41560 32.402 179 93 2 176 326 541 719 7.32e-20 92.0
MS.gene28552.t1 AT2G22950 31.638 177 92 3 177 324 650 826 7.86e-20 92.0
MS.gene28552.t1 AT3G57330 32.402 179 93 2 176 326 640 818 1.05e-19 91.7
MS.gene28552.t1 AT3G57330 32.402 179 93 2 176 326 585 763 1.08e-19 91.7
MS.gene28552.t1 AT3G60330 26.142 394 222 13 11 343 290 675 9.75e-19 88.6
MS.gene28552.t1 AT3G60330 26.142 394 222 13 11 343 290 675 9.75e-19 88.6
MS.gene28552.t1 AT3G60330 26.142 394 222 13 11 343 290 675 1.05e-18 88.6
MS.gene28552.t1 AT3G60330 26.142 394 222 13 11 343 290 675 1.05e-18 88.6
MS.gene28552.t1 AT5G57350 31.915 188 91 3 175 332 217 397 4.80e-18 86.3
MS.gene28552.t1 AT3G21180 27.717 184 100 2 176 326 691 874 5.05e-18 86.7
MS.gene28552.t1 AT4G30190 31.383 188 92 2 175 332 478 658 5.18e-18 86.7
MS.gene28552.t1 AT4G29900 27.143 210 119 3 150 326 653 861 5.31e-18 86.7
MS.gene28552.t1 AT4G29900 27.143 210 119 3 150 326 653 861 5.31e-18 86.7
MS.gene28552.t1 AT5G57350 31.915 188 91 3 175 332 479 659 5.47e-18 86.3
MS.gene28552.t1 AT5G57350 31.915 188 91 3 175 332 479 659 5.47e-18 86.3
MS.gene28552.t1 AT5G57350 31.915 188 91 3 175 332 479 659 5.47e-18 86.3
MS.gene28552.t1 AT2G18960 31.915 188 91 2 175 332 478 658 6.39e-18 86.3
MS.gene28552.t1 AT5G57110 27.717 184 100 2 176 326 674 857 6.55e-18 86.3
MS.gene28552.t1 AT5G57110 27.717 184 100 2 176 326 674 857 6.55e-18 86.3
MS.gene28552.t1 AT5G57110 27.717 184 100 2 176 326 674 857 6.55e-18 86.3
MS.gene28552.t1 AT2G18960 31.915 188 91 2 175 332 414 594 8.38e-18 85.9
MS.gene28552.t1 AT2G18960 31.915 188 91 2 175 332 414 594 8.38e-18 85.9
MS.gene28552.t1 AT1G17260 26.302 384 207 12 11 324 295 672 2.61e-17 84.3
MS.gene28552.t1 AT1G07670 27.528 178 97 2 176 321 621 798 2.84e-17 84.3
MS.gene28552.t1 AT1G07670 27.528 178 97 2 176 321 621 798 2.84e-17 84.3
MS.gene28552.t1 AT1G07810 27.528 178 97 2 176 321 621 798 3.97e-17 84.0
MS.gene28552.t1 AT3G47950 28.856 201 108 3 175 343 486 683 4.95e-17 83.6
MS.gene28552.t1 AT3G47950 28.856 201 108 3 175 343 486 683 4.95e-17 83.6
MS.gene28552.t1 AT2G07560 30.682 176 93 1 175 321 481 656 5.25e-17 83.6
MS.gene28552.t1 AT3G42640 30.348 201 105 3 175 343 481 678 6.52e-17 83.2
MS.gene28552.t1 AT4G30120 32.168 143 90 3 1 141 190 327 7.42e-17 81.6
MS.gene28552.t1 AT4G30120 32.168 143 90 3 1 141 349 486 8.97e-17 82.4
MS.gene28552.t1 AT5G62670 28.358 201 109 3 175 343 482 679 9.15e-17 82.8
MS.gene28552.t1 AT3G22910 28.261 184 99 2 176 326 636 819 1.61e-16 82.0
MS.gene28552.t1 AT2G24520 29.787 188 95 2 175 332 217 397 1.90e-16 81.6
MS.gene28552.t1 AT2G24520 29.787 188 95 2 175 332 523 703 1.98e-16 81.6
MS.gene28552.t1 AT2G24520 29.787 188 95 2 175 332 460 640 1.99e-16 81.6
MS.gene28552.t1 AT2G24520 29.787 188 95 2 175 332 460 640 1.99e-16 81.6
MS.gene28552.t1 AT1G10130 27.451 204 110 3 170 335 572 775 2.42e-16 81.3
MS.gene28552.t1 AT3G63380 27.184 206 116 3 155 326 618 823 4.74e-16 80.5
MS.gene28552.t1 AT1G80660 28.723 188 96 2 175 324 354 541 7.55e-16 79.7
MS.gene28552.t1 AT1G80660 28.723 188 96 2 175 324 483 670 9.08e-16 79.7
MS.gene28552.t1 AT1G80660 28.723 188 96 2 175 324 483 670 1.02e-15 79.3
MS.gene28552.t1 AT1G80660 28.723 188 96 2 175 324 483 670 1.05e-15 79.3
MS.gene28552.t1 AT4G00900 28.994 169 86 3 176 310 615 783 2.87e-14 75.1
MS.gene28552.t1 AT4G00900 28.994 169 86 3 176 310 615 783 2.87e-14 75.1
MS.gene28552.t1 AT4G30190 41.414 99 51 1 234 332 600 691 6.60e-13 70.9

Find 73 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAATTACATTTGGGCTTT+AGG 0.167127 8.4:-27519118 MS.gene28552:CDS
TAATGGTGACAGGTGATAAT+TGG 0.244399 8.4:-27520139 MS.gene28552:CDS
CTGAATAACTTGGAGATTAA+TGG 0.264775 8.4:-27518938 MS.gene28552:CDS
TATACTTGGTGGCAGAATTC+TGG 0.290037 8.4:-27519331 MS.gene28552:intron
TACTAAAGTAAAGGAATTAC+AGG 0.304850 8.4:-27520042 MS.gene28552:intron
ATAGCTTGTCCTTGTGCTCT+TGG 0.308206 8.4:-27520936 MS.gene28552:CDS
AGGTTACCACCTTGGATTGC+TGG 0.314307 8.4:-27519037 MS.gene28552:CDS
TCTTATGAAGAGTAACTTAG+AGG 0.322084 8.4:-27519191 MS.gene28552:CDS
AAAGAATTTGTGTCTATTGC+AGG 0.339636 8.4:-27520410 MS.gene28552:CDS
TGTTCAAAACAAGGAAATAA+TGG 0.352157 8.4:-27520369 MS.gene28552:CDS
TGGAGAAATTGTCGGAGTTT+TGG 0.354180 8.4:-27520234 MS.gene28552:CDS
TCAACAATGGACTTTGCTAT+TGG 0.359587 8.4:+27520488 None:intergenic
AAGACAGGAACTCTCACTAT+TGG 0.365137 8.4:-27520700 MS.gene28552:CDS
TATACGTCTAAATTACATTT+GGG 0.371723 8.4:-27519125 MS.gene28552:CDS
AATGGTGACAGGTGATAATT+GGG 0.389397 8.4:-27520138 MS.gene28552:CDS
GTATACGTCTAAATTACATT+TGG 0.391057 8.4:-27519126 MS.gene28552:CDS
GCTGAAAACATGGCTCAAAC+AGG 0.400125 8.4:-27520275 MS.gene28552:CDS
ACTTTAGTAGCTTTAGTCTC+AGG 0.403729 8.4:+27520056 None:intergenic
AAAAGTATGCCAGCAGCAAT+AGG 0.418214 8.4:+27519076 None:intergenic
CTAGCCATTCCTATTGCTGC+TGG 0.431313 8.4:-27519085 MS.gene28552:CDS
GGTAACCTAAATCTAGTAGA+AGG 0.435103 8.4:+27519052 None:intergenic
CATGAAAATAAAGAGCATAA+TGG 0.455425 8.4:-27520156 MS.gene28552:CDS
AGACAGGAACTCTCACTATT+GGG 0.460982 8.4:-27520699 MS.gene28552:CDS
TGTGTGCACTTTCTAATGCT+TGG 0.473648 8.4:+27520841 None:intergenic
GTTGGAATGGCAATTGGTGC+TGG 0.477758 8.4:-27519247 MS.gene28552:CDS
TTGGATTGCTGGAGCTGCAA+TGG 0.487123 8.4:-27519026 MS.gene28552:CDS
GCACTAGTAGCAGCTGATGT+TGG 0.489521 8.4:-27519265 MS.gene28552:CDS
TCCAAGTTATTCAGCTTGGT+TGG 0.493343 8.4:+27518947 None:intergenic
AATCTCCAAGTTATTCAGCT+TGG 0.494811 8.4:+27518943 None:intergenic
ACCAACCAAGCTGAATAACT+TGG 0.498362 8.4:-27518948 MS.gene28552:CDS
GCTGATGTTGGAATGGCAAT+TGG 0.508224 8.4:-27519253 MS.gene28552:CDS
AGCCACTCCTACAGCGGTCA+TGG 0.513949 8.4:-27520910 MS.gene28552:CDS
CTAGATTTAGGTTACCACCT+TGG 0.517511 8.4:-27519045 MS.gene28552:CDS
GTCATGGTTGGTACCGGAGT+TGG 0.522797 8.4:-27520894 MS.gene28552:CDS
GGATACACTGTAGGAATGGT+AGG 0.525626 8.4:-27519310 MS.gene28552:CDS
GACTAAAGCTACTAAAGTAA+AGG 0.529942 8.4:-27520051 MS.gene28552:CDS
AGCAGTGTGTTTGATAAGAC+AGG 0.531116 8.4:-27520715 MS.gene28552:CDS
ACAGCGGTCATGGTTGGTAC+CGG 0.534259 8.4:-27520900 MS.gene28552:CDS
CAAAACAAGGAAATAATGGT+TGG 0.535760 8.4:-27520365 MS.gene28552:CDS
ACATCAGCTGCTACTAGTGC+TGG 0.539899 8.4:+27519268 None:intergenic
ATAAAGAGCATAATGGTGAC+AGG 0.549153 8.4:-27520149 MS.gene28552:CDS
AATTCTATAGCTAGACAAGC+TGG 0.552536 8.4:-27520107 MS.gene28552:CDS
TTCTGGATACACTGTAGGAA+TGG 0.557596 8.4:-27519314 MS.gene28552:CDS
CAGAATTCTGGATACACTGT+AGG 0.561507 8.4:-27519319 MS.gene28552:CDS
ATGAACAAAATCATTCATCA+TGG 0.562767 8.4:-27520442 MS.gene28552:CDS
ACCTTGAGTTGCACCAACTC+CGG 0.571323 8.4:+27520881 None:intergenic
TTTGTGTCTATTGCAGGACA+TGG 0.575380 8.4:-27520404 MS.gene28552:CDS
TGACCGCTGTAGGAGTGGCT+AGG 0.576720 8.4:+27520913 None:intergenic
ACCGGAGTTGGTGCAACTCA+AGG 0.578148 8.4:-27520882 MS.gene28552:CDS
AGCATTAGAAAGTGCACACA+AGG 0.580174 8.4:-27520838 MS.gene28552:intron
ACTAGCAGAAGCTGAAAACA+TGG 0.583541 8.4:-27520285 MS.gene28552:CDS
AGTAGCAGCTGATGTTGGAA+TGG 0.584059 8.4:-27519260 MS.gene28552:CDS
GTGGCTAGGCCAAGAGCACA+AGG 0.584136 8.4:+27520927 None:intergenic
AACCATGACCGCTGTAGGAG+TGG 0.599538 8.4:+27520908 None:intergenic
TGGCCTAGCCACTCCTACAG+CGG 0.605944 8.4:-27520916 MS.gene28552:CDS
TGAACTTGTTGCTGCAGCAG+AGG 0.608008 8.4:-27520620 MS.gene28552:intron
CTGAAAACATGGCTCAAACA+GGG 0.609253 8.4:-27520274 MS.gene28552:CDS
TCATTGGATGGAGAAATTGT+CGG 0.612510 8.4:-27520242 MS.gene28552:CDS
ACTGTAGGAATGGTAGGAGA+TGG 0.612600 8.4:-27519304 MS.gene28552:CDS
CAAGGTGTGTTGATCAAAGG+TGG 0.614870 8.4:-27520864 MS.gene28552:CDS
ACTCCTACAGCGGTCATGGT+TGG 0.617392 8.4:-27520906 MS.gene28552:CDS
ATTGCAGCTCCAGCAATCCA+AGG 0.621783 8.4:+27519028 None:intergenic
TATTGAAACTGTCATGGCTG+AGG 0.621886 8.4:-27520084 MS.gene28552:CDS
GTACCAACCATGACCGCTGT+AGG 0.635820 8.4:+27520903 None:intergenic
TATTGCAGGACATGGAGTGA+AGG 0.637874 8.4:-27520396 MS.gene28552:CDS
TATGCCAGCAGCAATAGGAA+TGG 0.645015 8.4:+27519081 None:intergenic
GAAGGCAGTTGTTCAAAACA+AGG 0.645068 8.4:-27520378 MS.gene28552:CDS
TCATAACATAGCTATTTCAG+TGG 0.645859 8.4:-27520321 MS.gene28552:CDS
ACTCAAGGTGTGTTGATCAA+AGG 0.657349 8.4:-27520867 MS.gene28552:CDS
AGCTGGTATTGAAACTGTCA+TGG 0.661486 8.4:-27520090 MS.gene28552:CDS
TCTCACTATTGGGAAGCCAG+TGG 0.686617 8.4:-27520689 MS.gene28552:CDS
GCAGCTCCAGCAATCCAAGG+TGG 0.740644 8.4:+27519031 None:intergenic
ATGGTGACAGGTGATAATTG+GGG 0.767964 8.4:-27520137 MS.gene28552:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTAAAAAAAAAAAAATGTA+TGG - chr8.4:27520458-27520477 MS.gene28552:CDS 10.0%
!! TATTGTCTAAATATTATATA+GGG + chr8.4:27520501-27520520 None:intergenic 10.0%
!! TTATTGTCTAAATATTATAT+AGG + chr8.4:27520502-27520521 None:intergenic 10.0%
!! AATCTAAACAAAATAATTAG+AGG - chr8.4:27520157-27520176 MS.gene28552:CDS 15.0%
!! ATCTAAACAAAATAATTAGA+GGG - chr8.4:27520158-27520177 MS.gene28552:CDS 15.0%
!! TAATATTTGACATATATACT+TGG - chr8.4:27520519-27520538 MS.gene28552:intron 15.0%
!! ATTGTTATATCTACATGATT+AGG - chr8.4:27519339-27519358 MS.gene28552:intron 20.0%
!! TACAGGTATATACAAAATTA+TGG - chr8.4:27519839-27519858 MS.gene28552:intron 20.0%
!! TATACGTCTAAATTACATTT+GGG - chr8.4:27520739-27520758 MS.gene28552:intron 20.0%
!! TCTCAACAAAAAAATTTACA+AGG + chr8.4:27520004-27520023 None:intergenic 20.0%
!!! ATAGGACATTTATTCAATTT+TGG - chr8.4:27519931-27519950 MS.gene28552:intron 20.0%
!!! TATTTCAAGTACTACATTAA+TGG - chr8.4:27519281-27519300 MS.gene28552:CDS 20.0%
! AATCCCAACAAATACAATAA+AGG - chr8.4:27520027-27520046 MS.gene28552:intron 25.0%
! ACAACCTTTATTGTATTTGT+TGG + chr8.4:27520034-27520053 None:intergenic 25.0%
! ACACAAAAGAAAATATTTCG+AGG - chr8.4:27520320-27520339 MS.gene28552:CDS 25.0%
! ATCACTTAAGTTCAATTACT+TGG + chr8.4:27520189-27520208 None:intergenic 25.0%
! ATGAACAAAATCATTCATCA+TGG - chr8.4:27519422-27519441 MS.gene28552:intron 25.0%
! CAACCTTTATTGTATTTGTT+GGG + chr8.4:27520033-27520052 None:intergenic 25.0%
! CACAAAAGAAAATATTTCGA+GGG - chr8.4:27520321-27520340 MS.gene28552:CDS 25.0%
! GTATACGTCTAAATTACATT+TGG - chr8.4:27520738-27520757 MS.gene28552:intron 25.0%
! TACTAAAGTAAAGGAATTAC+AGG - chr8.4:27519822-27519841 MS.gene28552:intron 25.0%
! TATTTGACATATATACTTGG+TGG - chr8.4:27520522-27520541 MS.gene28552:intron 25.0%
! TGTTCAAAACAAGGAAATAA+TGG - chr8.4:27519495-27519514 MS.gene28552:intron 25.0%
! TTCTCATCAAGAATTGAATT+CGG + chr8.4:27520249-27520268 None:intergenic 25.0%
!! CATGAAAATAAAGAGCATAA+TGG - chr8.4:27519708-27519727 MS.gene28552:intron 25.0%
!! TTAATGAATTACCTCTAAGA+TGG - chr8.4:27520208-27520227 MS.gene28552:CDS 25.0%
!! TTCATCTTGTGTGATATTTT+TGG + chr8.4:27519407-27519426 None:intergenic 25.0%
!! TTTAAGACTTTGTTCACTAT+AGG - chr8.4:27519913-27519932 MS.gene28552:intron 25.0%
!! TTTTTCCTTCTACTAGATTT+AGG - chr8.4:27520807-27520826 MS.gene28552:intron 25.0%
!!! CATTATGCTCTTTATTTTCA+TGG + chr8.4:27519710-27519729 None:intergenic 25.0%
!!! TTTGTTACTTACAATTTTGC+AGG - chr8.4:27519121-27519140 MS.gene28552:CDS 25.0%
AAAGAATTTGTGTCTATTGC+AGG - chr8.4:27519454-27519473 MS.gene28552:intron 30.0%
AAAGGAGTGAAGCAATAATT+TGG + chr8.4:27519875-27519894 None:intergenic 30.0%
AAGCAATAATTTGGGAATTG+GGG + chr8.4:27519866-27519885 None:intergenic 30.0%
AAGGAGTGAAGCAATAATTT+GGG + chr8.4:27519874-27519893 None:intergenic 30.0%
AATTATTGCTTCACTCCTTT+AGG - chr8.4:27519875-27519894 MS.gene28552:intron 30.0%
AGTCTTTAACGTCAACTTAT+TGG - chr8.4:27520058-27520077 MS.gene28552:CDS 30.0%
ATTATTGCTTCACTCCTTTA+GGG - chr8.4:27519876-27519895 MS.gene28552:intron 30.0%
CAAAACAAGGAAATAATGGT+TGG - chr8.4:27519499-27519518 MS.gene28552:intron 30.0%
CAATCCTTACAAATTAAACG+CGG + chr8.4:27520137-27520156 None:intergenic 30.0%
CTAAATTACATTTGGGCTTT+AGG - chr8.4:27520746-27520765 MS.gene28552:intron 30.0%
CTGAATAACTTGGAGATTAA+TGG - chr8.4:27520926-27520945 MS.gene28552:CDS 30.0%
GAAGCAATAATTTGGGAATT+GGG + chr8.4:27519867-27519886 None:intergenic 30.0%
GACTAAAGCTACTAAAGTAA+AGG - chr8.4:27519813-27519832 MS.gene28552:intron 30.0%
TCAATGCAGTTATAGTGTAT+TGG - chr8.4:27519971-27519990 MS.gene28552:intron 30.0%
TCTTATGAAGAGTAACTTAG+AGG - chr8.4:27520673-27520692 MS.gene28552:CDS 30.0%
TGAAGCAATAATTTGGGAAT+TGG + chr8.4:27519868-27519887 None:intergenic 30.0%
! GAAATAACTTCTTTTGCATC+AGG + chr8.4:27519676-27519695 None:intergenic 30.0%
! TCATAACATAGCTATTTCAG+TGG - chr8.4:27519543-27519562 MS.gene28552:intron 30.0%
! TGACTTTTCTACAGTTGAAA+TGG + chr8.4:27519101-27519120 None:intergenic 30.0%
! TTCTTGCAATGATTGTGTTT+TGG + chr8.4:27520354-27520373 None:intergenic 30.0%
!! AACAGGGATTTTAGTATCAT+TGG - chr8.4:27519606-27519625 MS.gene28552:intron 30.0%
!!! ATTTTTGGCATGATCAACAA+TGG + chr8.4:27519392-27519411 None:intergenic 30.0%
AATCTCCAAGTTATTCAGCT+TGG + chr8.4:27520924-27520943 None:intergenic 35.0%
AATGGTGACAGGTGATAATT+GGG - chr8.4:27519726-27519745 MS.gene28552:intron 35.0%
AATTCTATAGCTAGACAAGC+TGG - chr8.4:27519757-27519776 MS.gene28552:intron 35.0%
GGTAACCTAAATCTAGTAGA+AGG + chr8.4:27520815-27520834 None:intergenic 35.0%
TAATGGTGACAGGTGATAAT+TGG - chr8.4:27519725-27519744 MS.gene28552:intron 35.0%
TCATTGGATGGAGAAATTGT+CGG - chr8.4:27519622-27519641 MS.gene28552:intron 35.0%
TGAATTCGGATTCTTCTGTA+CGG + chr8.4:27520235-27520254 None:intergenic 35.0%
! AGTTTTCTTTGCAAGGTCAA+TGG + chr8.4:27520711-27520730 None:intergenic 35.0%
! ATAAAGAGCATAATGGTGAC+AGG - chr8.4:27519715-27519734 MS.gene28552:intron 35.0%
! GACACAAATTCTTTTGCTTC+TGG + chr8.4:27519448-27519467 None:intergenic 35.0%
! GTGAGAAAGTTTTCTTTGCA+AGG + chr8.4:27520718-27520737 None:intergenic 35.0%
! TCAACAATGGACTTTGCTAT+TGG + chr8.4:27519379-27519398 None:intergenic 35.0%
!! ACTTTAGTAGCTTTAGTCTC+AGG + chr8.4:27519811-27519830 None:intergenic 35.0%
!! AGTTTTGTAGTTACAACCAC+TGG + chr8.4:27519194-27519213 None:intergenic 35.0%
!! GGGATTTTAGTATCATTGGA+TGG - chr8.4:27519610-27519629 MS.gene28552:intron 35.0%
!!! GTGTTAACACTCCGTTTTTT+AGG + chr8.4:27520304-27520323 None:intergenic 35.0%
AAGACAGGAACTCTCACTAT+TGG - chr8.4:27519164-27519183 MS.gene28552:CDS 40.0%
ACCAACCAAGCTGAATAACT+TGG - chr8.4:27520916-27520935 MS.gene28552:CDS 40.0%
ACTAGCAGAAGCTGAAAACA+TGG - chr8.4:27519579-27519598 MS.gene28552:intron 40.0%
AGACAGGAACTCTCACTATT+GGG - chr8.4:27519165-27519184 MS.gene28552:CDS 40.0%
AGCAGTGTGTTTGATAAGAC+AGG - chr8.4:27519149-27519168 MS.gene28552:CDS 40.0%
ATGGTGACAGGTGATAATTG+GGG - chr8.4:27519727-27519746 MS.gene28552:intron 40.0%
CAGAATTCTGGATACACTGT+AGG - chr8.4:27520545-27520564 MS.gene28552:intron 40.0%
CTGAAAACATGGCTCAAACA+GGG - chr8.4:27519590-27519609 MS.gene28552:intron 40.0%
GAAGGCAGTTGTTCAAAACA+AGG - chr8.4:27519486-27519505 MS.gene28552:intron 40.0%
TATTGAAACTGTCATGGCTG+AGG - chr8.4:27519780-27519799 MS.gene28552:intron 40.0%
TCCAAGTTATTCAGCTTGGT+TGG + chr8.4:27520920-27520939 None:intergenic 40.0%
TGTGTGCACTTTCTAATGCT+TGG + chr8.4:27519026-27519045 None:intergenic 40.0%
TTCTGGATACACTGTAGGAA+TGG - chr8.4:27520550-27520569 MS.gene28552:intron 40.0%
TTTGTGTCTATTGCAGGACA+TGG - chr8.4:27519460-27519479 MS.gene28552:intron 40.0%
! AAAAGTATGCCAGCAGCAAT+AGG + chr8.4:27520791-27520810 None:intergenic 40.0%
! ACCACGTGACGTGATTTTAT+TGG - chr8.4:27520081-27520100 MS.gene28552:CDS 40.0%
! ACTCAAGGTGTGTTGATCAA+AGG - chr8.4:27518997-27519016 MS.gene28552:CDS 40.0%
! AGCATTAGAAAGTGCACACA+AGG - chr8.4:27519026-27519045 MS.gene28552:CDS 40.0%
! AGCTGGTATTGAAACTGTCA+TGG - chr8.4:27519774-27519793 MS.gene28552:intron 40.0%
! ATGGCAAGTTGAGAGAACAA+TGG + chr8.4:27519082-27519101 None:intergenic 40.0%
! CCACGTGACGTGATTTTATT+GGG - chr8.4:27520082-27520101 MS.gene28552:CDS 40.0%
! CTAGATTTAGGTTACCACCT+TGG - chr8.4:27520819-27520838 MS.gene28552:intron 40.0%
! TATACTTGGTGGCAGAATTC+TGG - chr8.4:27520533-27520552 MS.gene28552:intron 40.0%
! TCTTTTGCATCAGGTTTCAG+TGG + chr8.4:27519667-27519686 None:intergenic 40.0%
! TGGAGAAATTGTCGGAGTTT+TGG - chr8.4:27519630-27519649 MS.gene28552:intron 40.0%
!! GCAACCGCGTTTAATTTGTA+AGG - chr8.4:27520130-27520149 MS.gene28552:CDS 40.0%
!!! ACACTCCGTTTTTTAGGAGA+AGG + chr8.4:27520298-27520317 None:intergenic 40.0%
!!! ACTCCGTTTTTTAGGAGAAG+GGG + chr8.4:27520296-27520315 None:intergenic 40.0%
!!! CACTCCGTTTTTTAGGAGAA+GGG + chr8.4:27520297-27520316 None:intergenic 40.0%
ACCATGAGAGCAAACCCTAA+AGG + chr8.4:27519893-27519912 None:intergenic 45.0%
ACTGTAGGAATGGTAGGAGA+TGG - chr8.4:27520560-27520579 MS.gene28552:intron 45.0%
AGTAGCAGCTGATGTTGGAA+TGG - chr8.4:27520604-27520623 MS.gene28552:intron 45.0%
CCCAATAAAATCACGTCACG+TGG + chr8.4:27520085-27520104 None:intergenic 45.0%
GCTGAAAACATGGCTCAAAC+AGG - chr8.4:27519589-27519608 MS.gene28552:intron 45.0%
GCTGATGTTGGAATGGCAAT+TGG - chr8.4:27520611-27520630 MS.gene28552:intron 45.0%
GGATACACTGTAGGAATGGT+AGG - chr8.4:27520554-27520573 MS.gene28552:intron 45.0%
TATGCCAGCAGCAATAGGAA+TGG + chr8.4:27520786-27520805 None:intergenic 45.0%
TATTGCAGGACATGGAGTGA+AGG - chr8.4:27519468-27519487 MS.gene28552:intron 45.0%
TGCCCCCTTCTCCTAAAAAA+CGG - chr8.4:27520290-27520309 MS.gene28552:CDS 45.0%
TTCTGTACGGACCATCTTAG+AGG + chr8.4:27520222-27520241 None:intergenic 45.0%
! ATAGCTTGTCCTTGTGCTCT+TGG - chr8.4:27518928-27518947 MS.gene28552:CDS 45.0%
! CAAGGTGTGTTGATCAAAGG+TGG - chr8.4:27519000-27519019 MS.gene28552:CDS 45.0%
!! TCCTTTAGGGTTTGCTCTCA+TGG - chr8.4:27519889-27519908 MS.gene28552:intron 45.0%
!!! CTCCGTTTTTTAGGAGAAGG+GGG + chr8.4:27520295-27520314 None:intergenic 45.0%
ACATCAGCTGCTACTAGTGC+TGG + chr8.4:27520599-27520618 None:intergenic 50.0%
AGGTTACCACCTTGGATTGC+TGG - chr8.4:27520827-27520846 MS.gene28552:intron 50.0%
ATTGCAGCTCCAGCAATCCA+AGG + chr8.4:27520839-27520858 None:intergenic 50.0%
CTAGCCATTCCTATTGCTGC+TGG - chr8.4:27520779-27520798 MS.gene28552:intron 50.0%
GCACTAGTAGCAGCTGATGT+TGG - chr8.4:27520599-27520618 MS.gene28552:intron 50.0%
GTTGGAATGGCAATTGGTGC+TGG - chr8.4:27520617-27520636 MS.gene28552:intron 50.0%
TCTCACTATTGGGAAGCCAG+TGG - chr8.4:27519175-27519194 MS.gene28552:CDS 50.0%
TTGGATTGCTGGAGCTGCAA+TGG - chr8.4:27520838-27520857 MS.gene28552:intron 50.0%
TTGTTGCTGCAGCAGAGGTA+AGG - chr8.4:27519249-27519268 MS.gene28552:CDS 50.0%
! ACCTTGAGTTGCACCAACTC+CGG + chr8.4:27518986-27519005 None:intergenic 50.0%
!! TGAACTTGTTGCTGCAGCAG+AGG - chr8.4:27519244-27519263 MS.gene28552:CDS 50.0%
AACCATGACCGCTGTAGGAG+TGG + chr8.4:27518959-27518978 None:intergenic 55.0%
ACTCCTACAGCGGTCATGGT+TGG - chr8.4:27518958-27518977 MS.gene28552:CDS 55.0%
GTACCAACCATGACCGCTGT+AGG + chr8.4:27518964-27518983 None:intergenic 55.0%
! ACAGCGGTCATGGTTGGTAC+CGG - chr8.4:27518964-27518983 MS.gene28552:CDS 55.0%
! GTCATGGTTGGTACCGGAGT+TGG - chr8.4:27518970-27518989 MS.gene28552:CDS 55.0%
!! ACCGGAGTTGGTGCAACTCA+AGG - chr8.4:27518982-27519001 MS.gene28552:CDS 55.0%
AGCCACTCCTACAGCGGTCA+TGG - chr8.4:27518954-27518973 MS.gene28552:CDS 60.0%
GCAGCTCCAGCAATCCAAGG+TGG + chr8.4:27520836-27520855 None:intergenic 60.0%
TGACCGCTGTAGGAGTGGCT+AGG + chr8.4:27518954-27518973 None:intergenic 60.0%
TGGCCTAGCCACTCCTACAG+CGG - chr8.4:27518948-27518967 MS.gene28552:CDS 60.0%
! GTGGCTAGGCCAAGAGCACA+AGG + chr8.4:27518940-27518959 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 27518922 27520964 27518922 ID=MS.gene28552
chr8.4 mRNA 27518922 27520964 27518922 ID=MS.gene28552.t1;Parent=MS.gene28552
chr8.4 exon 27520839 27520964 27520839 ID=MS.gene28552.t1.exon1;Parent=MS.gene28552.t1
chr8.4 CDS 27520839 27520964 27520839 ID=cds.MS.gene28552.t1;Parent=MS.gene28552.t1
chr8.4 exon 27520621 27520743 27520621 ID=MS.gene28552.t1.exon2;Parent=MS.gene28552.t1
chr8.4 CDS 27520621 27520743 27520621 ID=cds.MS.gene28552.t1;Parent=MS.gene28552.t1
chr8.4 exon 27520043 27520525 27520043 ID=MS.gene28552.t1.exon3;Parent=MS.gene28552.t1
chr8.4 CDS 27520043 27520525 27520043 ID=cds.MS.gene28552.t1;Parent=MS.gene28552.t1
chr8.4 exon 27518922 27519338 27518922 ID=MS.gene28552.t1.exon4;Parent=MS.gene28552.t1
chr8.4 CDS 27518922 27519338 27518922 ID=cds.MS.gene28552.t1;Parent=MS.gene28552.t1
Gene Sequence

>MS.gene28552

ATGGTCATAGCTTGTCCTTGTGCTCTTGGCCTAGCCACTCCTACAGCGGTCATGGTTGGTACCGGAGTTGGTGCAACTCAAGGTGTGTTGATCAAAGGTGGCCAAGCATTAGAAAGTGCACACAAGGTGAGTGAATAGCATTGACAGTTGCATGTATCCATTGTTCTCTCAACTTGCCATTTCAACTGTAGAAAAGTCATTTGTTACTTACAATTTTGCAGGTGAATAGCAGTGTGTTTGATAAGACAGGAACTCTCACTATTGGGAAGCCAGTGGTTGTAACTACAAAACTCTTCAATAACATGTCAGTTAAAGATTTCTATGAACTTGTTGCTGCAGCAGAGGTAAGGCTACATTAATATTTCAAGTACTACATTAATGGTTTATTCATAGCTGTAGCATTACTCAATAATGTTGATTGTTATATCTACATGATTAGGTGAATAGTGAACATCCAATAGCAAAGTCCATTGTTGATCATGCCAAAAATATCACACAAGATGAACAAAATCATTCATCATGGCCAGAAGCAAAAGAATTTGTGTCTATTGCAGGACATGGAGTGAAGGCAGTTGTTCAAAACAAGGAAATAATGGTTGGAAATAAAAAACTGATGTTAGATCATAACATAGCTATTTCAGTGGAAGCTGAAGAAATACTAGCAGAAGCTGAAAACATGGCTCAAACAGGGATTTTAGTATCATTGGATGGAGAAATTGTCGGAGTTTTGGCTGTGTCTGATCCACTGAAACCTGATGCAAAAGAAGTTATTTCTGTTCTTAAATCCATGAAAATAAAGAGCATAATGGTGACAGGTGATAATTGGGGAACTGCAAATTCTATAGCTAGACAAGCTGGTATTGAAACTGTCATGGCTGAGGCTCAACCTGAGACTAAAGCTACTAAAGTAAAGGAATTACAGGTATATACAAAATTATGGTCCCCAATTCCCAAATTATTGCTTCACTCCTTTAGGGTTTGCTCTCATGGTTTTAAGACTTTGTTCACTATAGGACATTTATTCAATTTTGGCTTAAATATGAGTTTAGTCAATGCAGTTATAGTGTATTGGTTTTAATCCTTGTAAATTTTTTTGTTGAGATTCAATCCCAACAAATACAATAAAGGTTGTTTTTAGTCTTTAACGTCAACTTATTGGACCACGTGACGTGATTTTATTGGGTCACGTATGACGTCAGAGACTAAAAGCAACCGCGTTTAATTTGTAAGGATTGAATCTAAACAAAATAATTAGAGGGATTAACCAAGTAATTGAACTTAAGTGATTAATGAATTACCTCTAAGATGGTCCGTACAGAAGAATCCGAATTCAATTCTTGATGAGAACAATTTTTCGTCAGACTTTAATGCCCCCTTCTCCTAAAAAACGGAGTGTTAACACAAAAGAAAATATTTCGAGGGACTAAAACCAAAACACAATCATTGCAAGAACTAAAAACATATTACTTTCATTTTTTATTCAATTTTTAGATTTTATATTTATTAAAATTGAACAAAATTTACTTAGAGACTACACTTAAAAAAAAAAAAATGTATGGAACAAAAATATATTCAACCCTATATAATATTTAGACAATAATATTTGACATATATACTTGGTGGCAGAATTCTGGATACACTGTAGGAATGGTAGGAGATGGTATCAATGATTCACCAGCACTAGTAGCAGCTGATGTTGGAATGGCAATTGGTGCTGGAACAGACATTGCTATTGAAGCAGCTGACATTGTTCTTATGAAGAGTAACTTAGAGGATATAATAATTGCCATTGACCTTGCAAAGAAAACTTTCTCACGTATACGTCTAAATTACATTTGGGCTTTAGGTTATAATATTCTAGCCATTCCTATTGCTGCTGGCATACTTTTTCCTTCTACTAGATTTAGGTTACCACCTTGGATTGCTGGAGCTGCAATGGCTGCTTCTTCTATCAGTGTTGTTTGCTCTTCTCTCTTGTTGAAGAATTACAAGAAACCAACCAAGCTGAATAACTTGGAGATTAATGGTATTCAGATTGAATAA
Protein sequence

>MS.gene28552.t1

MVIACPCALGLATPTAVMVGTGVGATQGVLIKGGQALESAHKVNSSVFDKTGTLTIGKPVVVTTKLFNNMSVKDFYELVAAAEVNSEHPIAKSIVDHAKNITQDEQNHSSWPEAKEFVSIAGHGVKAVVQNKEIMVGNKKLMLDHNIAISVEAEEILAEAENMAQTGILVSLDGEIVGVLAVSDPLKPDAKEVISVLKSMKIKSIMVTGDNWGTANSIARQAGIETVMAEAQPETKATKVKELQNSGYTVGMVGDGINDSPALVAADVGMAIGAGTDIAIEAADIVLMKSNLEDIIIAIDLAKKTFSRIRLNYIWALGYNILAIPIAAGILFPSTRFRLPPWIAGAAMAASSISVVCSSLLLKNYKKPTKLNNLEINGIQIE