AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050082


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050082.t1 MTR_5g064530 80.000 150 8 1 1 128 1 150 2.35e-58 177
MS.gene050082.t1 MTR_5g064510 78.512 121 10 1 1 105 1 121 1.09e-49 155
MS.gene050082.t1 MTR_4g093910 72.566 113 27 3 1 111 1 111 3.45e-32 110
MS.gene050082.t1 MTR_4g093950 72.566 113 27 3 1 111 1 111 5.10e-32 110
MS.gene050082.t1 MTR_4g093940 72.072 111 27 3 1 109 1 109 6.62e-32 109
MS.gene050082.t1 MTR_4g093920 72.072 111 26 4 1 109 1 108 1.69e-29 103
MS.gene050082.t1 MTR_4g094678 60.494 81 11 5 17 93 1 64 2.33e-14 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGGGAGTGTTGGATTAGT+AGG 0.289744 5.4:-50324563 None:intergenic
GAAAGGGATGGAGGAGATAA+TGG 0.324144 5.4:-50324642 None:intergenic
ATTGAAGGCAATGTTGGTTT+TGG 0.363932 5.4:-50324419 None:intergenic
TGAGGCAATGTTGGAATAGA+AGG 0.409708 5.4:-50324464 None:intergenic
GGGGTAGAGGTTGAAGGTGG+TGG 0.414326 5.4:-50324671 None:intergenic
AGTGAATTGGTTGGCAATGG+TGG 0.426352 5.4:-50324596 None:intergenic
GGAACATTGCCTTGAGGTTG+AGG 0.447102 5.4:-50324482 None:intergenic
AGGAATGTTGGGAAGTGAAT+TGG 0.464436 5.4:-50324609 None:intergenic
GAAGGTGGTGGAGAGAAGAA+AGG 0.468019 5.4:-50324659 None:intergenic
GGTTGAGATTGAAGGAATGT+TGG 0.470769 5.4:-50324621 None:intergenic
GTTGGATTAGTAGGAATGCT+AGG 0.470882 5.4:-50324554 None:intergenic
GGCAAAGGTGGCAATGTTGT+TGG 0.474544 5.4:-50324443 None:intergenic
GGCAATGTTGTTGGAATTGA+AGG 0.477009 5.4:-50324434 None:intergenic
GTTGTGTGGTTGTTTGCAAA+AGG 0.480622 5.4:-50324388 None:intergenic
CCTTGAGGTTGAGGCAATGT+TGG 0.482455 5.4:-50324473 None:intergenic
GAGATAATGGTTGAGATTGA+AGG 0.488067 5.4:-50324629 None:intergenic
AAGGTGGTGGAGAGAAGAAA+GGG 0.496086 5.4:-50324658 None:intergenic
ATGGTGGAAAGTTGAGAGAT+GGG 0.499488 5.4:-50324580 None:intergenic
GTTGGAATTGAAGGCAATGT+TGG 0.501730 5.4:-50324425 None:intergenic
GTTGAGATTGAAGGAATGTT+GGG 0.510751 5.4:-50324620 None:intergenic
TGGAGAGAAGAAAGGGATGG+AGG 0.512326 5.4:-50324651 None:intergenic
AAGAAGTAAAGATGTGATGA+TGG 0.515143 5.4:-50324327 None:intergenic
CTTCAAGGGGTAGAGGTTGA+AGG 0.516901 5.4:-50324677 None:intergenic
GGAAGTGAATTGGTTGGCAA+TGG 0.525163 5.4:-50324599 None:intergenic
AGTGGTGGCATAGTGAGCTT+AGG 0.545367 5.4:-50324530 None:intergenic
TGGTGGAGAGAAGAAAGGGA+TGG 0.548047 5.4:-50324654 None:intergenic
AAGTTGAGAGATGGGAGTGT+TGG 0.551170 5.4:-50324572 None:intergenic
ATAGTGAGCTTAGGCACTGA+AGG 0.554532 5.4:-50324521 None:intergenic
TTAGTAGGAATGCTAGGAAG+TGG 0.565420 5.4:-50324548 None:intergenic
GAGCTTAGGCACTGAAGGGA+TGG 0.575148 5.4:-50324516 None:intergenic
TTAGGCACTGAAGGGATGGT+AGG 0.587385 5.4:-50324512 None:intergenic
TTCTATGCTCATGTTTGACA+AGG 0.589337 5.4:-50324357 None:intergenic
AATGGTGGAAAGTTGAGAGA+TGG 0.590990 5.4:-50324581 None:intergenic
GAAGGGATGGTAGGCAATGG+AGG 0.598281 5.4:-50324503 None:intergenic
GTTGGAATAGAAGGCAAAGG+TGG 0.615611 5.4:-50324455 None:intergenic
CCAACATTGCCTCAACCTCA+AGG 0.620333 5.4:+50324473 MS.gene050082:CDS
TAGTGAGCTTAGGCACTGAA+GGG 0.628071 5.4:-50324520 None:intergenic
GTAGGAATGCTAGGAAGTGG+TGG 0.647666 5.4:-50324545 None:intergenic
ACTGAAGGGATGGTAGGCAA+TGG 0.655830 5.4:-50324506 None:intergenic
CAAGGGGTAGAGGTTGAAGG+TGG 0.657058 5.4:-50324674 None:intergenic
AATGTTGGAATAGAAGGCAA+AGG 0.665937 5.4:-50324458 None:intergenic
AATGGAGGAACATTGCCTTG+AGG 0.697803 5.4:-50324488 None:intergenic
ATGTTGGGAAGTGAATTGGT+TGG 0.737713 5.4:-50324605 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGAAGTAAAGATGTGATGA+TGG - chr5.4:50324330-50324349 None:intergenic 30.0%
!!! AATGTTGGTTTTGGCAAATT+GGG - chr5.4:50324413-50324432 None:intergenic 30.0%
AATGTTGGAATAGAAGGCAA+AGG - chr5.4:50324461-50324480 None:intergenic 35.0%
GTTGAGATTGAAGGAATGTT+GGG - chr5.4:50324623-50324642 None:intergenic 35.0%
TTCTATGCTCATGTTTGACA+AGG - chr5.4:50324360-50324379 None:intergenic 35.0%
! GAGATAATGGTTGAGATTGA+AGG - chr5.4:50324632-50324651 None:intergenic 35.0%
!!! ATTGAAGGCAATGTTGGTTT+TGG - chr5.4:50324422-50324441 None:intergenic 35.0%
!!! CAATGTTGGTTTTGGCAAAT+TGG - chr5.4:50324414-50324433 None:intergenic 35.0%
!!! TGTGATGATGGTTTTCATTG+AGG - chr5.4:50324318-50324337 None:intergenic 35.0%
AGGAATGTTGGGAAGTGAAT+TGG - chr5.4:50324612-50324631 None:intergenic 40.0%
ATGTTGGGAAGTGAATTGGT+TGG - chr5.4:50324608-50324627 None:intergenic 40.0%
GGCAATGTTGTTGGAATTGA+AGG - chr5.4:50324437-50324456 None:intergenic 40.0%
GGTTGAGATTGAAGGAATGT+TGG - chr5.4:50324624-50324643 None:intergenic 40.0%
GTTGGAATTGAAGGCAATGT+TGG - chr5.4:50324428-50324447 None:intergenic 40.0%
GTTGTGTGGTTGTTTGCAAA+AGG - chr5.4:50324391-50324410 None:intergenic 40.0%
TGAGGCAATGTTGGAATAGA+AGG - chr5.4:50324467-50324486 None:intergenic 40.0%
TTAGTAGGAATGCTAGGAAG+TGG - chr5.4:50324551-50324570 None:intergenic 40.0%
! AATGGTGGAAAGTTGAGAGA+TGG - chr5.4:50324584-50324603 None:intergenic 40.0%
! ATGGTGGAAAGTTGAGAGAT+GGG - chr5.4:50324583-50324602 None:intergenic 40.0%
!! GTTGGATTAGTAGGAATGCT+AGG - chr5.4:50324557-50324576 None:intergenic 40.0%
!! TTTTGGCAAATTGGGTTGTG+TGG - chr5.4:50324405-50324424 None:intergenic 40.0%
AATGGAGGAACATTGCCTTG+AGG - chr5.4:50324491-50324510 None:intergenic 45.0%
AGTGAATTGGTTGGCAATGG+TGG - chr5.4:50324599-50324618 None:intergenic 45.0%
GAAAGGGATGGAGGAGATAA+TGG - chr5.4:50324645-50324664 None:intergenic 45.0%
GGAAGTGAATTGGTTGGCAA+TGG - chr5.4:50324602-50324621 None:intergenic 45.0%
GTTGGAATAGAAGGCAAAGG+TGG - chr5.4:50324458-50324477 None:intergenic 45.0%
! AAGTTGAGAGATGGGAGTGT+TGG - chr5.4:50324575-50324594 None:intergenic 45.0%
!! AAGGTGGTGGAGAGAAGAAA+GGG - chr5.4:50324661-50324680 None:intergenic 45.0%
!! ATAGTGAGCTTAGGCACTGA+AGG - chr5.4:50324524-50324543 None:intergenic 45.0%
!! GATGGGAGTGTTGGATTAGT+AGG - chr5.4:50324566-50324585 None:intergenic 45.0%
!! TAGTGAGCTTAGGCACTGAA+GGG - chr5.4:50324523-50324542 None:intergenic 45.0%
ACTGAAGGGATGGTAGGCAA+TGG - chr5.4:50324509-50324528 None:intergenic 50.0%
AGTGGTGGCATAGTGAGCTT+AGG - chr5.4:50324533-50324552 None:intergenic 50.0%
CCAACATTGCCTCAACCTCA+AGG + chr5.4:50324473-50324492 MS.gene050082:CDS 50.0%
CCTTGAGGTTGAGGCAATGT+TGG - chr5.4:50324476-50324495 None:intergenic 50.0%
GGAACATTGCCTTGAGGTTG+AGG - chr5.4:50324485-50324504 None:intergenic 50.0%
GGCAAAGGTGGCAATGTTGT+TGG - chr5.4:50324446-50324465 None:intergenic 50.0%
GTAGGAATGCTAGGAAGTGG+TGG - chr5.4:50324548-50324567 None:intergenic 50.0%
TGGAGAGAAGAAAGGGATGG+AGG - chr5.4:50324654-50324673 None:intergenic 50.0%
TGGTGGAGAGAAGAAAGGGA+TGG - chr5.4:50324657-50324676 None:intergenic 50.0%
!! GAAGGTGGTGGAGAGAAGAA+AGG - chr5.4:50324662-50324681 None:intergenic 50.0%
!! TTAGGCACTGAAGGGATGGT+AGG - chr5.4:50324515-50324534 None:intergenic 50.0%
CAAGGGGTAGAGGTTGAAGG+TGG - chr5.4:50324677-50324696 None:intergenic 55.0%
GAAGGGATGGTAGGCAATGG+AGG - chr5.4:50324506-50324525 None:intergenic 55.0%
!! GAGCTTAGGCACTGAAGGGA+TGG - chr5.4:50324519-50324538 None:intergenic 55.0%
GGGGTAGAGGTTGAAGGTGG+TGG - chr5.4:50324674-50324693 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 50324311 50324697 50324311 ID=MS.gene050082
chr5.4 mRNA 50324311 50324697 50324311 ID=MS.gene050082.t1;Parent=MS.gene050082
chr5.4 exon 50324311 50324697 50324311 ID=MS.gene050082.t1.exon1;Parent=MS.gene050082.t1
chr5.4 CDS 50324311 50324697 50324311 ID=cds.MS.gene050082.t1;Parent=MS.gene050082.t1
Gene Sequence

>MS.gene050082

ATGGCCTCAATGAAAACCATCATCACATCTTTACTTCTTTTTGTTACCTTGTCAAACATGAGCATAGAAGCTCGCAACCTTTTGCAAACAACCACACAACCCAATTTGCCAAAACCAACATTGCCTTCAATTCCAACAACATTGCCACCTTTGCCTTCTATTCCAACATTGCCTCAACCTCAAGGCAATGTTCCTCCATTGCCTACCATCCCTTCAGTGCCTAAGCTCACTATGCCACCACTTCCTAGCATTCCTACTAATCCAACACTCCCATCTCTCAACTTTCCACCATTGCCAACCAATTCACTTCCCAACATTCCTTCAATCTCAACCATTATCTCCTCCATCCCTTTCTTCTCTCCACCACCTTCAACCTCTACCCCTTGA

Protein sequence

>MS.gene050082.t1

MASMKTIITSLLLFVTLSNMSIEARNLLQTTTQPNLPKPTLPSIPTTLPPLPSIPTLPQPQGNVPPLPTIPSVPKLTMPPLPSIPTNPTLPSLNFPPLPTNSLPNIPSISTIISSIPFFSPPPSTSTP