Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28624.t1 | XP_003626082.1 | 80.7 | 373 | 11 | 3 | 1 | 318 | 1 | 367 | 1.50E-160 | 575.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28624.t1 | Q8VZQ2 | 33.2 | 373 | 115 | 13 | 1 | 288 | 1 | 324 | 1.1e-32 | 142.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28624.t1 | B7FL77 | 80.7 | 373 | 11 | 3 | 1 | 318 | 1 | 367 | 1.1e-160 | 575.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene28624.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050718 | MS.gene28624 | 0.913076 | 9.35E-84 | -1.69E-46 |
| MS.gene050827 | MS.gene28624 | 0.931298 | 4.65E-94 | -1.69E-46 |
| MS.gene052665 | MS.gene28624 | 0.800523 | 1.38E-48 | -1.69E-46 |
| MS.gene059937 | MS.gene28624 | 0.809123 | 2.21E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28624.t1 | MTR_7g110830 | 80.697 | 373 | 11 | 3 | 1 | 318 | 1 | 367 | 0.0 | 570 |
| MS.gene28624.t1 | MTR_1g085640 | 50.255 | 392 | 89 | 18 | 1 | 309 | 1 | 369 | 4.66e-101 | 302 |
| MS.gene28624.t1 | MTR_1g085040 | 36.158 | 354 | 144 | 8 | 1 | 292 | 1 | 334 | 1.91e-49 | 169 |
| MS.gene28624.t1 | MTR_4g105130 | 47.020 | 151 | 26 | 2 | 1 | 98 | 1 | 150 | 2.29e-35 | 131 |
| MS.gene28624.t1 | MTR_1g112760 | 36.111 | 216 | 75 | 2 | 3 | 165 | 4 | 209 | 1.55e-29 | 112 |
| MS.gene28624.t1 | MTR_3g077110 | 44.366 | 142 | 25 | 2 | 1 | 88 | 1 | 142 | 3.95e-29 | 114 |
| MS.gene28624.t1 | MTR_7g111290 | 47.771 | 157 | 27 | 2 | 1 | 104 | 1 | 155 | 3.98e-29 | 115 |
| MS.gene28624.t1 | MTR_2g099740 | 39.098 | 133 | 28 | 1 | 1 | 80 | 1 | 133 | 1.37e-22 | 97.4 |
| MS.gene28624.t1 | MTR_8g017440 | 41.463 | 123 | 19 | 1 | 1 | 70 | 1 | 123 | 3.88e-22 | 95.5 |
| MS.gene28624.t1 | MTR_2g064160 | 40.000 | 130 | 24 | 2 | 1 | 77 | 1 | 129 | 2.83e-21 | 92.4 |
| MS.gene28624.t1 | MTR_8g017500 | 39.837 | 123 | 21 | 1 | 1 | 70 | 1 | 123 | 4.67e-21 | 92.4 |
| MS.gene28624.t1 | MTR_8g017540 | 40.650 | 123 | 20 | 1 | 1 | 70 | 47 | 169 | 7.38e-21 | 92.4 |
| MS.gene28624.t1 | MTR_4g478180 | 30.808 | 198 | 75 | 3 | 1 | 141 | 2 | 194 | 1.39e-20 | 91.3 |
| MS.gene28624.t1 | MTR_3g074520 | 35.606 | 132 | 32 | 1 | 1 | 79 | 1 | 132 | 1.34e-19 | 88.2 |
| MS.gene28624.t1 | MTR_6g012690 | 37.500 | 128 | 27 | 1 | 1 | 75 | 1 | 128 | 1.80e-19 | 88.2 |
| MS.gene28624.t1 | MTR_6g009430 | 34.848 | 132 | 33 | 1 | 1 | 79 | 1 | 132 | 2.10e-19 | 87.4 |
| MS.gene28624.t1 | MTR_4g073420 | 29.282 | 181 | 68 | 2 | 1 | 128 | 1 | 174 | 2.10e-19 | 86.7 |
| MS.gene28624.t1 | MTR_8g017480 | 75.556 | 45 | 11 | 0 | 1 | 45 | 1 | 45 | 5.84e-19 | 79.3 |
| MS.gene28624.t1 | MTR_7g011170 | 37.121 | 132 | 30 | 1 | 1 | 79 | 1 | 132 | 6.95e-19 | 85.9 |
| MS.gene28624.t1 | MTR_8g095390 | 36.364 | 132 | 31 | 1 | 1 | 79 | 1 | 132 | 7.12e-19 | 85.9 |
| MS.gene28624.t1 | MTR_4g082230 | 35.156 | 128 | 30 | 1 | 1 | 75 | 1 | 128 | 1.31e-18 | 85.9 |
| MS.gene28624.t1 | MTR_5g078140 | 34.483 | 145 | 42 | 1 | 1 | 92 | 1 | 145 | 2.05e-18 | 84.7 |
| MS.gene28624.t1 | MTR_8g098860 | 38.136 | 118 | 20 | 1 | 1 | 65 | 1 | 118 | 2.15e-18 | 84.3 |
| MS.gene28624.t1 | MTR_5g010020 | 44.048 | 84 | 45 | 1 | 1 | 82 | 1 | 84 | 7.08e-18 | 83.2 |
| MS.gene28624.t1 | MTR_3g065440 | 37.692 | 130 | 28 | 1 | 1 | 77 | 1 | 130 | 1.43e-17 | 82.0 |
| MS.gene28624.t1 | MTR_3g097450 | 33.588 | 131 | 34 | 1 | 1 | 78 | 1 | 131 | 6.01e-17 | 80.1 |
| MS.gene28624.t1 | MTR_5g029840 | 26.897 | 290 | 116 | 10 | 1 | 229 | 1 | 255 | 7.21e-17 | 80.5 |
| MS.gene28624.t1 | MTR_5g007370 | 36.667 | 120 | 23 | 1 | 12 | 78 | 12 | 131 | 1.35e-16 | 79.0 |
| MS.gene28624.t1 | MTR_8g017350 | 37.903 | 124 | 23 | 2 | 1 | 70 | 1 | 124 | 2.42e-16 | 80.1 |
| MS.gene28624.t1 | MTR_8g017340 | 37.903 | 124 | 23 | 2 | 1 | 70 | 1 | 124 | 2.53e-16 | 79.7 |
| MS.gene28624.t1 | MTR_8g017390 | 39.316 | 117 | 17 | 2 | 1 | 63 | 1 | 117 | 3.48e-16 | 79.3 |
| MS.gene28624.t1 | MTR_4g102380 | 34.351 | 131 | 33 | 1 | 1 | 78 | 1 | 131 | 1.52e-15 | 75.9 |
| MS.gene28624.t1 | MTR_3g461490 | 31.818 | 154 | 49 | 2 | 1 | 101 | 1 | 151 | 1.60e-15 | 76.3 |
| MS.gene28624.t1 | MTR_4g091490 | 33.333 | 129 | 32 | 2 | 1 | 75 | 1 | 129 | 4.80e-15 | 75.1 |
| MS.gene28624.t1 | MTR_1g100653 | 31.609 | 174 | 58 | 3 | 1 | 115 | 1 | 172 | 6.77e-15 | 74.3 |
| MS.gene28624.t1 | MTR_8g031360 | 37.121 | 132 | 28 | 3 | 1 | 77 | 1 | 132 | 2.29e-14 | 72.8 |
| MS.gene28624.t1 | MTR_7g076740 | 33.835 | 133 | 34 | 3 | 1 | 79 | 1 | 133 | 4.96e-14 | 71.6 |
| MS.gene28624.t1 | MTR_5g079670 | 32.000 | 125 | 32 | 1 | 3 | 74 | 2 | 126 | 9.46e-14 | 69.7 |
| MS.gene28624.t1 | MTR_1g100667 | 36.975 | 119 | 21 | 2 | 9 | 74 | 10 | 127 | 6.80e-13 | 68.2 |
| MS.gene28624.t1 | MTR_7g096930 | 33.898 | 118 | 25 | 1 | 1 | 65 | 1 | 118 | 1.78e-12 | 67.0 |
| MS.gene28624.t1 | MTR_6g055910 | 37.288 | 118 | 20 | 2 | 9 | 72 | 11 | 128 | 3.77e-12 | 66.2 |
| MS.gene28624.t1 | MTR_4g065017 | 33.929 | 112 | 21 | 1 | 11 | 69 | 16 | 127 | 4.20e-12 | 66.2 |
| MS.gene28624.t1 | MTR_7g115650 | 28.667 | 150 | 43 | 2 | 1 | 97 | 1 | 139 | 4.29e-12 | 64.7 |
| MS.gene28624.t1 | MTR_2g097910 | 34.906 | 106 | 16 | 1 | 12 | 64 | 20 | 125 | 6.25e-12 | 65.9 |
| MS.gene28624.t1 | MTR_3g028740 | 34.862 | 109 | 18 | 1 | 9 | 64 | 19 | 127 | 2.89e-11 | 63.9 |
| MS.gene28624.t1 | MTR_5g007310 | 46.835 | 79 | 37 | 2 | 45 | 123 | 6 | 79 | 9.37e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28624.t1 | AT4G01680 | 49.032 | 155 | 26 | 1 | 1 | 102 | 1 | 155 | 8.61e-41 | 142 |
| MS.gene28624.t1 | AT4G01680 | 49.032 | 155 | 26 | 1 | 1 | 102 | 1 | 155 | 2.32e-40 | 144 |
| MS.gene28624.t1 | AT1G09540 | 48.993 | 149 | 23 | 1 | 1 | 96 | 1 | 149 | 6.77e-38 | 138 |
| MS.gene28624.t1 | AT1G57560 | 53.788 | 132 | 8 | 1 | 1 | 79 | 1 | 132 | 1.04e-37 | 136 |
| MS.gene28624.t1 | AT1G57560 | 53.383 | 133 | 9 | 1 | 1 | 80 | 1 | 133 | 3.18e-37 | 134 |
| MS.gene28624.t1 | AT5G26660 | 48.649 | 148 | 21 | 2 | 1 | 93 | 1 | 148 | 4.21e-37 | 136 |
| MS.gene28624.t1 | AT4G01680 | 95.556 | 45 | 2 | 0 | 1 | 45 | 1 | 45 | 2.63e-24 | 101 |
| MS.gene28624.t1 | AT4G01680 | 45.652 | 92 | 48 | 2 | 12 | 102 | 77 | 167 | 6.64e-17 | 80.5 |
| MS.gene28624.t1 | AT3G02940 | 38.519 | 135 | 30 | 1 | 1 | 82 | 1 | 135 | 6.51e-23 | 97.1 |
| MS.gene28624.t1 | AT3G02940 | 38.519 | 135 | 30 | 1 | 1 | 82 | 1 | 135 | 9.37e-23 | 96.7 |
| MS.gene28624.t1 | AT3G02940 | 38.519 | 135 | 30 | 1 | 1 | 82 | 1 | 135 | 9.37e-23 | 96.7 |
| MS.gene28624.t1 | AT5G16770 | 37.778 | 135 | 31 | 1 | 1 | 82 | 1 | 135 | 9.19e-22 | 94.0 |
| MS.gene28624.t1 | AT5G16770 | 37.778 | 135 | 31 | 1 | 1 | 82 | 1 | 135 | 9.19e-22 | 94.0 |
| MS.gene28624.t1 | AT5G16770 | 37.778 | 135 | 31 | 1 | 1 | 82 | 1 | 135 | 1.43e-21 | 93.6 |
| MS.gene28624.t1 | AT5G16770 | 37.778 | 135 | 31 | 1 | 1 | 82 | 1 | 135 | 1.43e-21 | 93.6 |
| MS.gene28624.t1 | AT3G13890 | 42.241 | 116 | 14 | 1 | 1 | 63 | 1 | 116 | 2.12e-21 | 93.6 |
| MS.gene28624.t1 | AT3G12720 | 40.476 | 126 | 21 | 2 | 1 | 73 | 12 | 136 | 3.94e-21 | 92.0 |
| MS.gene28624.t1 | AT1G66230 | 39.130 | 138 | 31 | 1 | 1 | 85 | 1 | 138 | 1.11e-20 | 90.5 |
| MS.gene28624.t1 | AT1G34670 | 37.121 | 132 | 30 | 1 | 1 | 79 | 1 | 132 | 1.11e-20 | 91.7 |
| MS.gene28624.t1 | AT5G15310 | 37.879 | 132 | 29 | 1 | 1 | 79 | 1 | 132 | 1.26e-19 | 88.2 |
| MS.gene28624.t1 | AT5G16600 | 36.111 | 144 | 39 | 1 | 1 | 91 | 1 | 144 | 1.36e-19 | 88.2 |
| MS.gene28624.t1 | AT5G15310 | 37.879 | 132 | 29 | 1 | 1 | 79 | 1 | 132 | 1.39e-19 | 87.8 |
| MS.gene28624.t1 | AT3G13890 | 44.086 | 93 | 41 | 1 | 1 | 82 | 1 | 93 | 1.63e-19 | 88.2 |
| MS.gene28624.t1 | AT5G15310 | 37.879 | 132 | 29 | 1 | 1 | 79 | 1 | 132 | 3.75e-19 | 85.5 |
| MS.gene28624.t1 | AT1G63910 | 36.719 | 128 | 28 | 1 | 1 | 75 | 1 | 128 | 5.97e-19 | 86.7 |
| MS.gene28624.t1 | AT3G61250 | 37.984 | 129 | 27 | 1 | 1 | 76 | 1 | 129 | 8.52e-19 | 85.5 |
| MS.gene28624.t1 | AT4G34990 | 30.464 | 151 | 52 | 1 | 1 | 98 | 1 | 151 | 1.86e-18 | 84.0 |
| MS.gene28624.t1 | AT5G07690 | 34.286 | 140 | 39 | 1 | 1 | 87 | 1 | 140 | 2.25e-18 | 84.7 |
| MS.gene28624.t1 | AT3G01140 | 37.121 | 132 | 30 | 1 | 1 | 79 | 1 | 132 | 2.38e-18 | 84.7 |
| MS.gene28624.t1 | AT5G61420 | 34.532 | 139 | 36 | 2 | 1 | 84 | 1 | 139 | 2.53e-18 | 85.1 |
| MS.gene28624.t1 | AT2G16720 | 30.128 | 156 | 56 | 1 | 1 | 103 | 1 | 156 | 8.33e-18 | 82.0 |
| MS.gene28624.t1 | AT4G21440 | 25.938 | 320 | 132 | 9 | 1 | 236 | 1 | 299 | 1.14e-17 | 82.8 |
| MS.gene28624.t1 | AT5G60890 | 32.558 | 129 | 34 | 1 | 1 | 76 | 1 | 129 | 2.33e-17 | 81.3 |
| MS.gene28624.t1 | AT5G10280 | 33.333 | 132 | 35 | 1 | 1 | 79 | 1 | 132 | 2.44e-17 | 81.6 |
| MS.gene28624.t1 | AT4G22680 | 35.766 | 137 | 34 | 2 | 1 | 83 | 1 | 137 | 4.11e-17 | 80.1 |
| MS.gene28624.t1 | AT4G12350 | 29.240 | 171 | 68 | 1 | 1 | 118 | 1 | 171 | 7.82e-17 | 79.7 |
| MS.gene28624.t1 | AT4G38620 | 32.824 | 131 | 35 | 1 | 1 | 78 | 1 | 131 | 9.49e-17 | 79.3 |
| MS.gene28624.t1 | AT5G65230 | 35.115 | 131 | 32 | 1 | 1 | 78 | 1 | 131 | 1.61e-16 | 79.0 |
| MS.gene28624.t1 | AT4G09460 | 32.593 | 135 | 38 | 1 | 1 | 82 | 1 | 135 | 2.59e-16 | 77.4 |
| MS.gene28624.t1 | AT4G05100 | 34.109 | 129 | 31 | 2 | 1 | 75 | 1 | 129 | 2.84e-16 | 78.6 |
| MS.gene28624.t1 | AT5G26660 | 47.059 | 85 | 41 | 3 | 12 | 93 | 34 | 117 | 1.26e-15 | 76.6 |
| MS.gene28624.t1 | AT1G22640 | 32.061 | 131 | 36 | 1 | 1 | 78 | 1 | 131 | 1.38e-15 | 75.5 |
| MS.gene28624.t1 | AT3G13540 | 33.099 | 142 | 41 | 2 | 6 | 94 | 17 | 157 | 3.04e-15 | 74.7 |
| MS.gene28624.t1 | AT5G56110 | 33.333 | 132 | 35 | 1 | 1 | 79 | 1 | 132 | 3.04e-15 | 75.5 |
| MS.gene28624.t1 | AT1G18570 | 32.824 | 131 | 34 | 2 | 1 | 77 | 1 | 131 | 3.25e-15 | 75.9 |
| MS.gene28624.t1 | AT5G14340 | 29.480 | 173 | 62 | 4 | 1 | 119 | 13 | 179 | 3.39e-15 | 74.7 |
| MS.gene28624.t1 | AT1G74430 | 35.156 | 128 | 30 | 1 | 1 | 75 | 1 | 128 | 3.46e-15 | 74.7 |
| MS.gene28624.t1 | AT5G07700 | 31.538 | 130 | 36 | 1 | 1 | 77 | 1 | 130 | 7.75e-15 | 74.7 |
| MS.gene28624.t1 | AT5G07700 | 31.538 | 130 | 36 | 1 | 1 | 77 | 1 | 130 | 7.75e-15 | 74.7 |
| MS.gene28624.t1 | AT3G28470 | 34.848 | 132 | 33 | 1 | 1 | 79 | 1 | 132 | 2.87e-14 | 72.8 |
| MS.gene28624.t1 | AT1G74080 | 31.783 | 129 | 35 | 1 | 1 | 76 | 1 | 129 | 7.31e-14 | 71.6 |
| MS.gene28624.t1 | AT3G48920 | 54.237 | 59 | 25 | 1 | 9 | 65 | 15 | 73 | 8.39e-14 | 70.5 |
| MS.gene28624.t1 | AT1G18710 | 46.154 | 65 | 35 | 0 | 12 | 76 | 65 | 129 | 6.63e-13 | 68.2 |
| MS.gene28624.t1 | AT2G31180 | 25.381 | 197 | 94 | 1 | 1 | 144 | 1 | 197 | 9.04e-13 | 67.4 |
| MS.gene28624.t1 | AT1G79180 | 33.333 | 117 | 25 | 1 | 2 | 65 | 4 | 120 | 1.29e-11 | 64.7 |
| MS.gene28624.t1 | AT1G74430 | 65.000 | 40 | 13 | 1 | 37 | 75 | 20 | 59 | 2.67e-11 | 62.4 |
| MS.gene28624.t1 | AT5G14340 | 51.020 | 49 | 23 | 1 | 32 | 79 | 6 | 54 | 3.71e-11 | 61.6 |
| MS.gene28624.t1 | AT4G25560 | 35.185 | 108 | 17 | 1 | 8 | 62 | 6 | 113 | 7.23e-11 | 62.4 |
| MS.gene28624.t1 | AT3G08500 | 33.333 | 117 | 22 | 2 | 4 | 64 | 19 | 135 | 8.43e-11 | 62.8 |
| MS.gene28624.t1 | AT5G61420 | 58.824 | 51 | 18 | 2 | 37 | 84 | 10 | 60 | 9.16e-11 | 62.0 |
Find 65 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGAAGAGTTTGGATTATT+TGG | 0.116986 | 7.2:+4692261 | None:intergenic |
| GATTTAGGCCCTGATGGTTT+AGG | 0.252849 | 7.2:-4691886 | MS.gene28624:CDS |
| TTGAATCATATTACTAAATA+TGG | 0.254773 | 7.2:-4693162 | MS.gene28624:CDS |
| TCTTATAATATAGCAGCTTT+TGG | 0.280061 | 7.2:-4691823 | MS.gene28624:CDS |
| GATAAGATACTATTCTTCAT+AGG | 0.304611 | 7.2:+4692060 | None:intergenic |
| ATTTAGGCCCTGATGGTTTA+GGG | 0.313997 | 7.2:-4691885 | MS.gene28624:CDS |
| AGAATAGTATCTTATCTTCT+TGG | 0.317415 | 7.2:-4692053 | MS.gene28624:CDS |
| ATAAAGCCTGCCACAGATTT+AGG | 0.320366 | 7.2:-4691901 | MS.gene28624:CDS |
| ATAATGAAATAAAGAATCTT+TGG | 0.322110 | 7.2:-4692605 | MS.gene28624:CDS |
| GAATAGTATCTTATCTTCTT+GGG | 0.325673 | 7.2:-4692052 | MS.gene28624:CDS |
| TGAAGATTGTTTAGGCTCAT+AGG | 0.335997 | 7.2:+4692392 | None:intergenic |
| TTGAACCAATGAGAAGAGTT+TGG | 0.356074 | 7.2:+4692252 | None:intergenic |
| ATAGGAAACAGCATCTGATT+TGG | 0.364879 | 7.2:+4692410 | None:intergenic |
| GGAAGAAATGAGGTATTAGT+TGG | 0.366998 | 7.2:+4692156 | None:intergenic |
| CAAAGGTTATTCAACTGAAA+TGG | 0.369898 | 7.2:-4692361 | MS.gene28624:CDS |
| AGGACAATTGAAGATTGTTT+AGG | 0.372326 | 7.2:+4692384 | None:intergenic |
| ATATTTCTGCTGCTTTCTCT+TGG | 0.373195 | 7.2:+4692475 | None:intergenic |
| TTCTTGTTCCAACTATTTGA+TGG | 0.388346 | 7.2:-4692328 | MS.gene28624:CDS |
| TCTCATTGGTTCAACCAAAC+AGG | 0.390750 | 7.2:-4692243 | MS.gene28624:CDS |
| GATGTGTAATTCATCTGAAT+AGG | 0.392658 | 7.2:+4692297 | None:intergenic |
| ATAATCCAAACTCTTCTCAT+TGG | 0.399767 | 7.2:-4692257 | MS.gene28624:CDS |
| GTAATTTCCCATCAAATAGT+TGG | 0.401184 | 7.2:+4692320 | None:intergenic |
| TTGAGAAGATTCTTGTTGCT+TGG | 0.413002 | 7.2:+4691846 | None:intergenic |
| AAGTACTTTCTGATCTCATA+AGG | 0.429540 | 7.2:+4692433 | None:intergenic |
| AAAGAAGAAACTGAGACAAA+AGG | 0.438499 | 7.2:-4692571 | MS.gene28624:CDS |
| AATTCAGAGTTCATGTCAAA+AGG | 0.443007 | 7.2:+4692213 | None:intergenic |
| ATTATGTCCCCTAAACCATC+AGG | 0.449694 | 7.2:+4691877 | None:intergenic |
| GAAGAAATGAGGTATTAGTT+GGG | 0.458948 | 7.2:+4692157 | None:intergenic |
| TTTCAGTTGAATAACCTTTG+AGG | 0.461827 | 7.2:+4692364 | None:intergenic |
| GAAAGTGGTTTATGAGTGAC+AGG | 0.465506 | 7.2:+4692540 | None:intergenic |
| TGTTTGATTCTCTTTAATAG+AGG | 0.466549 | 7.2:+4692011 | None:intergenic |
| AAGAAGAAACTGAGACAAAA+GGG | 0.474216 | 7.2:-4692570 | MS.gene28624:CDS |
| CTAAATATGGTCATGGATGT+TGG | 0.475014 | 7.2:-4693149 | MS.gene28624:CDS |
| GTTGCTACAAGCAGAAGCTT+AGG | 0.481348 | 7.2:-4693221 | MS.gene28624:CDS |
| TAATATATACCTGCTTGCTT+AGG | 0.482505 | 7.2:+4693117 | None:intergenic |
| GCCACAGATTTAGGCCCTGA+TGG | 0.492844 | 7.2:-4691892 | MS.gene28624:CDS |
| TCTTGTTCCAACTATTTGAT+GGG | 0.507060 | 7.2:-4692327 | MS.gene28624:CDS |
| ATAAGATACTATTCTTCATA+GGG | 0.510246 | 7.2:+4692061 | None:intergenic |
| ATGTGTAATTCATCTGAATA+GGG | 0.511653 | 7.2:+4692298 | None:intergenic |
| GGTAGAAAATGTTGATGAAG+AGG | 0.516385 | 7.2:-4692514 | MS.gene28624:CDS |
| AGAAGCTTAGGAAAGGTCTA+TGG | 0.519654 | 7.2:-4693209 | MS.gene28624:CDS |
| AAAAGTATTGGGAAGAAATG+AGG | 0.537696 | 7.2:+4692146 | None:intergenic |
| CAATCTTCAATTGTCCTCAA+AGG | 0.538889 | 7.2:-4692378 | MS.gene28624:CDS |
| CATATTACTAAATATGGTCA+TGG | 0.549148 | 7.2:-4693156 | MS.gene28624:CDS |
| AGGTCTATGGTCACCTGAGG+AGG | 0.556622 | 7.2:-4693196 | MS.gene28624:CDS |
| TTATTCAACTGAAATGGAAG+TGG | 0.560042 | 7.2:-4692355 | MS.gene28624:CDS |
| AATAGTATCTTATCTTCTTG+GGG | 0.560835 | 7.2:-4692051 | MS.gene28624:CDS |
| TCAGGGCCTAAATCTGTGGC+AGG | 0.565813 | 7.2:+4691895 | None:intergenic |
| TCAACTGAAATGGAAGTGGA+AGG | 0.579991 | 7.2:-4692351 | MS.gene28624:CDS |
| GTGAAGATACTCATTCCACT+TGG | 0.581545 | 7.2:+4691975 | None:intergenic |
| AGTTCAGTTCCTAAGCAAGC+AGG | 0.581863 | 7.2:-4693126 | MS.gene28624:intron |
| TTTAGGCCCTGATGGTTTAG+GGG | 0.584861 | 7.2:-4691884 | MS.gene28624:CDS |
| CACTCATAAACCACTTTCTG+AGG | 0.586110 | 7.2:-4692535 | MS.gene28624:CDS |
| GACATAGAAACATCTTGTGA+AGG | 0.586534 | 7.2:+4692096 | None:intergenic |
| ATTATTTGGAATATTATCAG+TGG | 0.586790 | 7.2:+4692275 | None:intergenic |
| AAGAAATGAGGTATTAGTTG+GGG | 0.597506 | 7.2:+4692158 | None:intergenic |
| GAAGGAACATCATCAAGTGA+TGG | 0.602005 | 7.2:+4692114 | None:intergenic |
| CAGAAGAAGAAGAAGCCAAG+TGG | 0.603165 | 7.2:-4691990 | MS.gene28624:CDS |
| ACCATCAGGGCCTAAATCTG+TGG | 0.616487 | 7.2:+4691891 | None:intergenic |
| GATTCTTGTTGCTTGGAATG+TGG | 0.641176 | 7.2:+4691853 | None:intergenic |
| TACAAGCAGAAGCTTAGGAA+AGG | 0.647918 | 7.2:-4693216 | MS.gene28624:CDS |
| GAAAGGTCTATGGTCACCTG+AGG | 0.676025 | 7.2:-4693199 | MS.gene28624:CDS |
| TTATGTCCCCTAAACCATCA+GGG | 0.688532 | 7.2:+4691878 | None:intergenic |
| AGAAATGAGGTATTAGTTGG+GGG | 0.699843 | 7.2:+4692159 | None:intergenic |
| AAAGTGGTTTATGAGTGACA+GGG | 0.778051 | 7.2:+4692541 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACATTATCATAATAAGA+AGG | + | chr7.2:4692215-4692234 | None:intergenic | 15.0% |
| !! | ATAATGAAATAAAGAATCTT+TGG | - | chr7.2:4692445-4692464 | MS.gene28624:CDS | 15.0% |
| !! | GAGATTGAATTATTAAAATT+TGG | + | chr7.2:4691967-4691986 | None:intergenic | 15.0% |
| !! | TTGAATCATATTACTAAATA+TGG | - | chr7.2:4691888-4691907 | MS.gene28624:CDS | 15.0% |
| !!! | TATTTGTCAATGTATTTTAT+TGG | - | chr7.2:4692039-4692058 | MS.gene28624:CDS | 15.0% |
| !!! | TGTATTTTTTAATTGTTATG+TGG | - | chr7.2:4692321-4692340 | MS.gene28624:CDS | 15.0% |
| !! | ATAAGATACTATTCTTCATA+GGG | + | chr7.2:4692992-4693011 | None:intergenic | 20.0% |
| !! | ATTATCATAATAAGAAGGAT+TGG | + | chr7.2:4692210-4692229 | None:intergenic | 20.0% |
| !!! | ATTATTTGGAATATTATCAG+TGG | + | chr7.2:4692778-4692797 | None:intergenic | 20.0% |
| ! | AATAGTATCTTATCTTCTTG+GGG | - | chr7.2:4692999-4693018 | MS.gene28624:intron | 25.0% |
| ! | AGAATAGTATCTTATCTTCT+TGG | - | chr7.2:4692997-4693016 | MS.gene28624:intron | 25.0% |
| ! | ATGTGTAATTCATCTGAATA+GGG | + | chr7.2:4692755-4692774 | None:intergenic | 25.0% |
| ! | CATATTACTAAATATGGTCA+TGG | - | chr7.2:4691894-4691913 | MS.gene28624:CDS | 25.0% |
| ! | GAATAGTATCTTATCTTCTT+GGG | - | chr7.2:4692998-4693017 | MS.gene28624:intron | 25.0% |
| ! | GATAAGATACTATTCTTCAT+AGG | + | chr7.2:4692993-4693012 | None:intergenic | 25.0% |
| ! | TCTCTTGATTATATGCTAAA+AGG | + | chr7.2:4691989-4692008 | None:intergenic | 25.0% |
| ! | TGTTTGATTCTCTTTAATAG+AGG | + | chr7.2:4693042-4693061 | None:intergenic | 25.0% |
| ! | TTTAATTGTTATGTGGTTGT+TGG | - | chr7.2:4692328-4692347 | MS.gene28624:CDS | 25.0% |
| !! | AATGTACCTCTTTTTAAATC+AGG | + | chr7.2:4692123-4692142 | None:intergenic | 25.0% |
| !! | ATATGATGTGTTTTCTTAGA+TGG | - | chr7.2:4692388-4692407 | MS.gene28624:CDS | 25.0% |
| !! | TCTTATAATATAGCAGCTTT+TGG | - | chr7.2:4693227-4693246 | MS.gene28624:CDS | 25.0% |
| !! | TGATTTTGTAGCATTAACAT+TGG | + | chr7.2:4693104-4693123 | None:intergenic | 25.0% |
| AAAGAAGAAACTGAGACAAA+AGG | - | chr7.2:4692479-4692498 | MS.gene28624:CDS | 30.0% | |
| AAGAAGAAACTGAGACAAAA+GGG | - | chr7.2:4692480-4692499 | MS.gene28624:CDS | 30.0% | |
| AAGTACTTTCTGATCTCATA+AGG | + | chr7.2:4692620-4692639 | None:intergenic | 30.0% | |
| AATTCAGAGTTCATGTCAAA+AGG | + | chr7.2:4692840-4692859 | None:intergenic | 30.0% | |
| AGGACAATTGAAGATTGTTT+AGG | + | chr7.2:4692669-4692688 | None:intergenic | 30.0% | |
| ATAATCCAAACTCTTCTCAT+TGG | - | chr7.2:4692793-4692812 | MS.gene28624:intron | 30.0% | |
| ATTGAACTTCATTCAGTACT+AGG | - | chr7.2:4692168-4692187 | MS.gene28624:CDS | 30.0% | |
| CAAAGGTTATTCAACTGAAA+TGG | - | chr7.2:4692689-4692708 | MS.gene28624:intron | 30.0% | |
| GATGTGTAATTCATCTGAAT+AGG | + | chr7.2:4692756-4692775 | None:intergenic | 30.0% | |
| GTAATTTCCCATCAAATAGT+TGG | + | chr7.2:4692733-4692752 | None:intergenic | 30.0% | |
| TAATATATACCTGCTTGCTT+AGG | + | chr7.2:4691936-4691955 | None:intergenic | 30.0% | |
| TCTTGTTCCAACTATTTGAT+GGG | - | chr7.2:4692723-4692742 | MS.gene28624:intron | 30.0% | |
| TGAGAAGAGTTTGGATTATT+TGG | + | chr7.2:4692792-4692811 | None:intergenic | 30.0% | |
| TTATTCAACTGAAATGGAAG+TGG | - | chr7.2:4692695-4692714 | MS.gene28624:intron | 30.0% | |
| TTCTTGTTCCAACTATTTGA+TGG | - | chr7.2:4692722-4692741 | MS.gene28624:intron | 30.0% | |
| TTGAACTTCATTCAGTACTA+GGG | - | chr7.2:4692169-4692188 | MS.gene28624:CDS | 30.0% | |
| TTTCAGTTGAATAACCTTTG+AGG | + | chr7.2:4692689-4692708 | None:intergenic | 30.0% | |
| ! | AAGAAATGAGGTATTAGTTG+GGG | + | chr7.2:4692895-4692914 | None:intergenic | 30.0% |
| ! | GAAGAAATGAGGTATTAGTT+GGG | + | chr7.2:4692896-4692915 | None:intergenic | 30.0% |
| ! | TCAATGTATTTTATTGGCAC+AGG | - | chr7.2:4692045-4692064 | MS.gene28624:CDS | 30.0% |
| ! | TGGTTTGTAGCAAAAAGTAT+TGG | + | chr7.2:4692919-4692938 | None:intergenic | 30.0% |
| !! | AAAAGTATTGGGAAGAAATG+AGG | + | chr7.2:4692907-4692926 | None:intergenic | 30.0% |
| !! | CAAAAGGTTTTAATCCTGTT+TGG | + | chr7.2:4692824-4692843 | None:intergenic | 30.0% |
| !! | GGTTTGTAGCAAAAAGTATT+GGG | + | chr7.2:4692918-4692937 | None:intergenic | 30.0% |
| !! | TTAGGTGGATTAATTACTTG+AGG | - | chr7.2:4692097-4692116 | MS.gene28624:CDS | 30.0% |
| AAAGTGGTTTATGAGTGACA+GGG | + | chr7.2:4692512-4692531 | None:intergenic | 35.0% | |
| ACATCATATGCATGTCAACA+AGG | + | chr7.2:4692377-4692396 | None:intergenic | 35.0% | |
| ATAGGAAACAGCATCTGATT+TGG | + | chr7.2:4692643-4692662 | None:intergenic | 35.0% | |
| CAATCTTCAATTGTCCTCAA+AGG | - | chr7.2:4692672-4692691 | MS.gene28624:intron | 35.0% | |
| CTAAATATGGTCATGGATGT+TGG | - | chr7.2:4691901-4691920 | MS.gene28624:CDS | 35.0% | |
| GACATAGAAACATCTTGTGA+AGG | + | chr7.2:4692957-4692976 | None:intergenic | 35.0% | |
| TGAAGATTGTTTAGGCTCAT+AGG | + | chr7.2:4692661-4692680 | None:intergenic | 35.0% | |
| TTCATTCAGTACTAGGGAAT+AGG | - | chr7.2:4692175-4692194 | MS.gene28624:CDS | 35.0% | |
| TTGAACCAATGAGAAGAGTT+TGG | + | chr7.2:4692801-4692820 | None:intergenic | 35.0% | |
| TTGAGAAGATTCTTGTTGCT+TGG | + | chr7.2:4693207-4693226 | None:intergenic | 35.0% | |
| TTGAGGCCTGATTTAAAAAG+AGG | - | chr7.2:4692114-4692133 | MS.gene28624:CDS | 35.0% | |
| ! | AAAAGTTTTTCATCCTCCTC+AGG | + | chr7.2:4691870-4691889 | None:intergenic | 35.0% |
| ! | ACATTTTCTACCTCAGAAAG+TGG | + | chr7.2:4692528-4692547 | None:intergenic | 35.0% |
| ! | AGAAATGAGGTATTAGTTGG+GGG | + | chr7.2:4692894-4692913 | None:intergenic | 35.0% |
| ! | GGAAGAAATGAGGTATTAGT+TGG | + | chr7.2:4692897-4692916 | None:intergenic | 35.0% |
| ! | GGTAGAAAATGTTGATGAAG+AGG | - | chr7.2:4692536-4692555 | MS.gene28624:CDS | 35.0% |
| !! | ATATTTCTGCTGCTTTCTCT+TGG | + | chr7.2:4692578-4692597 | None:intergenic | 35.0% |
| AAATTGCTGCACAATTGCCA+GGG | - | chr7.2:4692415-4692434 | MS.gene28624:CDS | 40.0% | |
| AGAAGCTTAGGAAAGGTCTA+TGG | - | chr7.2:4691841-4691860 | MS.gene28624:CDS | 40.0% | |
| ATAAAGCCTGCCACAGATTT+AGG | - | chr7.2:4693149-4693168 | MS.gene28624:CDS | 40.0% | |
| ATTATGTCCCCTAAACCATC+AGG | + | chr7.2:4693176-4693195 | None:intergenic | 40.0% | |
| ATTTAGGCCCTGATGGTTTA+GGG | - | chr7.2:4693165-4693184 | MS.gene28624:CDS | 40.0% | |
| ATTTCATTATCAGTCCTCCC+TGG | + | chr7.2:4692435-4692454 | None:intergenic | 40.0% | |
| CACTCATAAACCACTTTCTG+AGG | - | chr7.2:4692515-4692534 | MS.gene28624:CDS | 40.0% | |
| GAAAGTGGTTTATGAGTGAC+AGG | + | chr7.2:4692513-4692532 | None:intergenic | 40.0% | |
| GAAGGAACATCATCAAGTGA+TGG | + | chr7.2:4692939-4692958 | None:intergenic | 40.0% | |
| GTGAAGATACTCATTCCACT+TGG | + | chr7.2:4693078-4693097 | None:intergenic | 40.0% | |
| TACAAGCAGAAGCTTAGGAA+AGG | - | chr7.2:4691834-4691853 | MS.gene28624:CDS | 40.0% | |
| TCAACTGAAATGGAAGTGGA+AGG | - | chr7.2:4692699-4692718 | MS.gene28624:intron | 40.0% | |
| TTATGTCCCCTAAACCATCA+GGG | + | chr7.2:4693175-4693194 | None:intergenic | 40.0% | |
| TTATTGGCACAGGTCTTCAA+AGG | - | chr7.2:4692055-4692074 | MS.gene28624:CDS | 40.0% | |
| ! | GATTCTTGTTGCTTGGAATG+TGG | + | chr7.2:4693200-4693219 | None:intergenic | 40.0% |
| ! | TGTGTGAGTGTTGTAAACTC+TGG | + | chr7.2:4692268-4692287 | None:intergenic | 40.0% |
| !! | TCTCATTGGTTCAACCAAAC+AGG | - | chr7.2:4692807-4692826 | MS.gene28624:intron | 40.0% |
| AGTTCAGTTCCTAAGCAAGC+AGG | - | chr7.2:4691924-4691943 | MS.gene28624:CDS | 45.0% | |
| CAAATTGCTGCACAATTGCC+AGG | - | chr7.2:4692414-4692433 | MS.gene28624:CDS | 45.0% | |
| CAGAAGAAGAAGAAGCCAAG+TGG | - | chr7.2:4693060-4693079 | MS.gene28624:intron | 45.0% | |
| GATTTAGGCCCTGATGGTTT+AGG | - | chr7.2:4693164-4693183 | MS.gene28624:CDS | 45.0% | |
| GTTGCTACAAGCAGAAGCTT+AGG | - | chr7.2:4691829-4691848 | MS.gene28624:CDS | 45.0% | |
| TTTAGGCCCTGATGGTTTAG+GGG | - | chr7.2:4693166-4693185 | MS.gene28624:CDS | 45.0% | |
| ! | AAAGGTGTGGTAAGAGTTGC+AGG | - | chr7.2:4692073-4692092 | MS.gene28624:CDS | 45.0% |
| ACACCAGAGTCTAACGCAAG+TGG | + | chr7.2:4692304-4692323 | None:intergenic | 50.0% | |
| ACCATCAGGGCCTAAATCTG+TGG | + | chr7.2:4693162-4693181 | None:intergenic | 50.0% | |
| CAACCACTTGCGTTAGACTC+TGG | - | chr7.2:4692298-4692317 | MS.gene28624:CDS | 50.0% | |
| CAGAGTCTAACGCAAGTGGT+TGG | + | chr7.2:4692300-4692319 | None:intergenic | 50.0% | |
| GAAAGGTCTATGGTCACCTG+AGG | - | chr7.2:4691851-4691870 | MS.gene28624:CDS | 50.0% | |
| GTAAGAGTTGCAGGCTTAGG+TGG | - | chr7.2:4692082-4692101 | MS.gene28624:CDS | 50.0% | |
| GTGGTAAGAGTTGCAGGCTT+AGG | - | chr7.2:4692079-4692098 | MS.gene28624:CDS | 50.0% | |
| AGGTCTATGGTCACCTGAGG+AGG | - | chr7.2:4691854-4691873 | MS.gene28624:CDS | 55.0% | |
| GCCACAGATTTAGGCCCTGA+TGG | - | chr7.2:4693158-4693177 | MS.gene28624:CDS | 55.0% | |
| GGCACAGGTCTTCAAAGGTG+TGG | - | chr7.2:4692060-4692079 | MS.gene28624:CDS | 55.0% | |
| TCAGGGCCTAAATCTGTGGC+AGG | + | chr7.2:4693158-4693177 | None:intergenic | 55.0% | |
| TTGCTGCACAATTGCCAGGG+AGG | - | chr7.2:4692418-4692437 | MS.gene28624:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 4691813 | 4693259 | 4691813 | ID=MS.gene28624 |
| chr7.2 | mRNA | 4691813 | 4693259 | 4691813 | ID=MS.gene28624.t1;Parent=MS.gene28624 |
| chr7.2 | exon | 4693127 | 4693259 | 4693127 | ID=MS.gene28624.t1.exon1;Parent=MS.gene28624.t1 |
| chr7.2 | CDS | 4693127 | 4693259 | 4693127 | ID=cds.MS.gene28624.t1;Parent=MS.gene28624.t1 |
| chr7.2 | exon | 4691813 | 4692636 | 4691813 | ID=MS.gene28624.t1.exon2;Parent=MS.gene28624.t1 |
| chr7.2 | CDS | 4691813 | 4692636 | 4691813 | ID=cds.MS.gene28624.t1;Parent=MS.gene28624.t1 |
| Gene Sequence |
| Protein sequence |
