Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28662.t1 | XP_027188490.1 | 100 | 97 | 0 | 0 | 1 | 97 | 191 | 287 | 3.40E-50 | 207.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28662.t1 | G7KVH9 | 100.0 | 97 | 0 | 0 | 1 | 97 | 259 | 355 | 2.4e-50 | 207.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059007 | MS.gene28662 | 0.811513 | 6.74E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28662.t1 | MTR_7g111080 | 100.000 | 97 | 0 | 0 | 1 | 97 | 259 | 355 | 4.69e-63 | 206 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28662.t1 | AT5G24710 | 91.753 | 97 | 8 | 0 | 1 | 97 | 261 | 357 | 5.44e-59 | 194 |
Find 32 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGCCACAGTGTGGCTTCT+TGG | 0.158323 | 7.1:+3979162 | MS.gene28662:CDS |
| ATGGGACACGGTCTCCTTTA+AGG | 0.178355 | 7.1:+3979014 | MS.gene28662:CDS |
| ACACTGTGGCAGGCCAATTT+TGG | 0.221087 | 7.1:-3979152 | None:intergenic |
| TATAAAGCCAGTTCCAAAAT+TGG | 0.291327 | 7.1:+3979139 | MS.gene28662:CDS |
| CAGATAAGACATTAGCTATA+TGG | 0.304586 | 7.1:+3978995 | MS.gene28662:CDS |
| AGCACCAATACATACCTTAA+AGG | 0.347234 | 7.1:-3979028 | None:intergenic |
| GGCACATGACGGCGGGGTTG+TGG | 0.393623 | 7.1:+3978918 | None:intergenic |
| AGATAAGACATTAGCTATAT+GGG | 0.473469 | 7.1:+3978996 | MS.gene28662:CDS |
| GCTCCTCAGCTAATTACAAT+TGG | 0.477705 | 7.1:+3978970 | MS.gene28662:CDS |
| TGACACAGGTTAGAATATCA+AGG | 0.478884 | 7.1:-3979205 | None:intergenic |
| CCAAGAAGCCACACTGTGGC+AGG | 0.482982 | 7.1:-3979162 | None:intergenic |
| GCATCTAGGCACATGACGGC+GGG | 0.484753 | 7.1:+3978911 | None:intergenic |
| AGTTGAGCTTTCTAGAGTGA+TGG | 0.494949 | 7.1:+3978942 | MS.gene28662:CDS |
| CCACATATTCTGCTCTCACT+AGG | 0.510622 | 7.1:+3979374 | MS.gene28662:CDS |
| CAGGTTAGAATATCAAGGTT+CGG | 0.521517 | 7.1:-3979200 | None:intergenic |
| GTTGAGCTTTCTAGAGTGAT+GGG | 0.525416 | 7.1:+3978943 | MS.gene28662:CDS |
| CCTAGTGAGAGCAGAATATG+TGG | 0.528222 | 7.1:-3979374 | None:intergenic |
| TACGTGAGAATCTTTGACAC+AGG | 0.575126 | 7.1:-3979219 | None:intergenic |
| GTGTCAAAGATTCTCACGTA+TGG | 0.581676 | 7.1:+3979222 | MS.gene28662:CDS |
| ATATCAAGGTTCGGAGCTCG+AGG | 0.588597 | 7.1:-3979191 | None:intergenic |
| TGCATCTAGGCACATGACGG+CGG | 0.607347 | 7.1:+3978910 | None:intergenic |
| GCGCCAATTGTAATTAGCTG+AGG | 0.640157 | 7.1:-3978973 | None:intergenic |
| CAAGGTTCGGAGCTCGAGGG+TGG | 0.647667 | 7.1:-3979187 | None:intergenic |
| CATCTAGGCACATGACGGCG+GGG | 0.653225 | 7.1:+3978912 | None:intergenic |
| TATCAAGGTTCGGAGCTCGA+GGG | 0.657549 | 7.1:-3979190 | None:intergenic |
| GACATTAGCTATATGGGACA+CGG | 0.665115 | 7.1:+3979002 | MS.gene28662:CDS |
| TGATGCATCTAGGCACATGA+CGG | 0.671546 | 7.1:+3978907 | None:intergenic |
| AAAATTGGCCTGCCACAGTG+TGG | 0.682317 | 7.1:+3979154 | MS.gene28662:CDS |
| GAGCTTTCTAGAGTGATGGG+AGG | 0.688391 | 7.1:+3978946 | MS.gene28662:CDS |
| ACGGTAGTCAATTCACACAA+TGG | 0.696769 | 7.1:-3979397 | None:intergenic |
| GGCACCAAGAAGCCACACTG+TGG | 0.725339 | 7.1:-3979166 | None:intergenic |
| CTAGTGAGAGCAGAATATGT+GGG | 0.736342 | 7.1:-3979373 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTGTTTGAATTTTTGTTA+GGG | + | chr7.1:3979339-3979358 | MS.gene28662:intron | 20.0% |
| !!! | TCTTTGTTTGAATTTTTGTT+AGG | + | chr7.1:3979338-3979357 | MS.gene28662:intron | 20.0% |
| ! | AAGAAGTCTTCAAAACTATA+TGG | - | chr7.1:3979322-3979341 | None:intergenic | 25.0% |
| ! | AGATAAGACATTAGCTATAT+GGG | + | chr7.1:3978996-3979015 | MS.gene28662:CDS | 25.0% |
| CAGATAAGACATTAGCTATA+TGG | + | chr7.1:3978995-3979014 | MS.gene28662:CDS | 30.0% | |
| TATAAAGCCAGTTCCAAAAT+TGG | + | chr7.1:3979139-3979158 | MS.gene28662:CDS | 30.0% | |
| !!! | TTAGCTTGCTTTTAATTCTC+AGG | + | chr7.1:3979106-3979125 | MS.gene28662:intron | 30.0% |
| AGCACCAATACATACCTTAA+AGG | - | chr7.1:3979031-3979050 | None:intergenic | 35.0% | |
| CAGGTTAGAATATCAAGGTT+CGG | - | chr7.1:3979203-3979222 | None:intergenic | 35.0% | |
| TGACACAGGTTAGAATATCA+AGG | - | chr7.1:3979208-3979227 | None:intergenic | 35.0% | |
| ! | ATGCAGTTTTCTTTCTTCTG+TGG | + | chr7.1:3979246-3979265 | MS.gene28662:intron | 35.0% |
| ! | TAAGTCTTTCTAGTCATGAG+AGG | - | chr7.1:3979083-3979102 | None:intergenic | 35.0% |
| !! | GTCTCCTTTAAGGTATGTAT+TGG | + | chr7.1:3979024-3979043 | MS.gene28662:intron | 35.0% |
| CTAGTGAGAGCAGAATATGT+GGG | - | chr7.1:3979376-3979395 | None:intergenic | 40.0% | |
| GACATTAGCTATATGGGACA+CGG | + | chr7.1:3979002-3979021 | MS.gene28662:CDS | 40.0% | |
| GCTCCTCAGCTAATTACAAT+TGG | + | chr7.1:3978970-3978989 | MS.gene28662:CDS | 40.0% | |
| GTGTCAAAGATTCTCACGTA+TGG | + | chr7.1:3979222-3979241 | MS.gene28662:CDS | 40.0% | |
| TACGTGAGAATCTTTGACAC+AGG | - | chr7.1:3979222-3979241 | None:intergenic | 40.0% | |
| !! | AGTTGAGCTTTCTAGAGTGA+TGG | + | chr7.1:3978942-3978961 | MS.gene28662:CDS | 40.0% |
| !! | CTTTTAATTCTCAGGAGCTG+CGG | + | chr7.1:3979114-3979133 | MS.gene28662:intron | 40.0% |
| !! | GTTGAGCTTTCTAGAGTGAT+GGG | + | chr7.1:3978943-3978962 | MS.gene28662:CDS | 40.0% |
| CCACATATTCTGCTCTCACT+AGG | + | chr7.1:3979374-3979393 | MS.gene28662:CDS | 45.0% | |
| CCTAGTGAGAGCAGAATATG+TGG | - | chr7.1:3979377-3979396 | None:intergenic | 45.0% | |
| GCGCCAATTGTAATTAGCTG+AGG | - | chr7.1:3978976-3978995 | None:intergenic | 45.0% | |
| !!! | TTTTAATAGAATTAAAATAA+AGG | - | chr7.1:3979058-3979077 | None:intergenic | 5.0% |
| ATATCAAGGTTCGGAGCTCG+AGG | - | chr7.1:3979194-3979213 | None:intergenic | 50.0% | |
| ATGGGACACGGTCTCCTTTA+AGG | + | chr7.1:3979014-3979033 | MS.gene28662:CDS | 50.0% | |
| TATCAAGGTTCGGAGCTCGA+GGG | - | chr7.1:3979193-3979212 | None:intergenic | 50.0% | |
| ! | AAAATTGGCCTGCCACAGTG+TGG | + | chr7.1:3979154-3979173 | MS.gene28662:CDS | 50.0% |
| ! | ACACTGTGGCAGGCCAATTT+TGG | - | chr7.1:3979155-3979174 | None:intergenic | 50.0% |
| !! | GAGCTTTCTAGAGTGATGGG+AGG | + | chr7.1:3978946-3978965 | MS.gene28662:CDS | 50.0% |
| !!! | TGGCAGGCCAATTTTGGAAC+TGG | - | chr7.1:3979149-3979168 | None:intergenic | 50.0% |
| CCAAGAAGCCACACTGTGGC+AGG | - | chr7.1:3979165-3979184 | None:intergenic | 60.0% | |
| CCTGCCACAGTGTGGCTTCT+TGG | + | chr7.1:3979162-3979181 | MS.gene28662:CDS | 60.0% | |
| GGCACCAAGAAGCCACACTG+TGG | - | chr7.1:3979169-3979188 | None:intergenic | 60.0% | |
| CAAGGTTCGGAGCTCGAGGG+TGG | - | chr7.1:3979190-3979209 | None:intergenic | 65.0% | |
| CACAACCCCGCCGTCATGTG+CGG | - | chr7.1:3978920-3978939 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 3978919 | 3979417 | 3978919 | ID=MS.gene28662 |
| chr7.1 | mRNA | 3978919 | 3979417 | 3978919 | ID=MS.gene28662.t1;Parent=MS.gene28662 |
| chr7.1 | exon | 3978919 | 3979035 | 3978919 | ID=MS.gene28662.t1.exon1;Parent=MS.gene28662.t1 |
| chr7.1 | CDS | 3978919 | 3979035 | 3978919 | ID=cds.MS.gene28662.t1;Parent=MS.gene28662.t1 |
| chr7.1 | exon | 3979128 | 3979243 | 3979128 | ID=MS.gene28662.t1.exon2;Parent=MS.gene28662.t1 |
| chr7.1 | CDS | 3979128 | 3979243 | 3979128 | ID=cds.MS.gene28662.t1;Parent=MS.gene28662.t1 |
| chr7.1 | exon | 3979360 | 3979417 | 3979360 | ID=MS.gene28662.t1.exon3;Parent=MS.gene28662.t1 |
| chr7.1 | CDS | 3979360 | 3979417 | 3979360 | ID=cds.MS.gene28662.t1;Parent=MS.gene28662.t1 |
| Gene Sequence |
| Protein sequence |
