AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059007


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059007.t1 MTR_1g069105 99.387 163 1 0 1 163 1 163 2.00e-115 332
MS.gene059007.t1 MTR_1g069105 99.387 163 1 0 1 163 1 163 8.87e-115 332
MS.gene059007.t1 MTR_7g099640 92.073 164 10 2 1 163 1 162 1.23e-102 301
MS.gene059007.t1 MTR_7g099640 92.073 164 10 2 1 163 1 162 2.25e-102 301
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059007.t1 AT4G29040 89.571 163 14 1 1 163 1 160 3.02e-100 295
MS.gene059007.t1 AT2G20140 88.957 163 15 1 1 163 1 160 1.07e-99 294
MS.gene059007.t1 AT5G58290 34.043 94 58 2 67 159 33 123 4.11e-11 60.5

Find 58 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCGCTGCCGTCTTGTTTC+CGG 0.187678 1.2:-51861512 None:intergenic
GGTGAAGGATTATTTGTTGA+TGG 0.316417 1.2:+51861680 MS.gene059007:CDS
GCCGATGAGTGTTGGGAATC+TGG 0.341792 1.2:+51861794 MS.gene059007:CDS
GCCGTCTTGTTTCCGGTCAC+CGG 0.353994 1.2:-51861505 None:intergenic
TCATGCTATTGTTTCGAGTT+CGG 0.405752 1.2:+51861836 MS.gene059007:CDS
GGCGGAGGAAGATAGATCTA+AGG 0.407560 1.2:+51861752 MS.gene059007:CDS
GGTCACCGGGATGGCCTTGT+CGG 0.424135 1.2:-51861491 None:intergenic
GCTATTGTTTCGAGTTCGGT+TGG 0.424882 1.2:+51861840 MS.gene059007:CDS
GCCTTGTCGGTTCATGCCGC+CGG 0.428871 1.2:-51861478 None:intergenic
TGTTTCGAGTTCGGTTGGGC+CGG 0.432915 1.2:+51861845 MS.gene059007:CDS
CTCAGCTTGCACTTCGATAC+CGG 0.441459 1.2:-51861639 None:intergenic
TCGTAAACAACGCAAACAAA+AGG 0.444206 1.2:+51861581 MS.gene059007:CDS
GTGGCTCGCCGATGAGTGTT+GGG 0.452232 1.2:+51861787 MS.gene059007:CDS
ACACGCGCCGGTGGTGCTGC+TGG 0.453673 1.2:-51861555 None:intergenic
CCGTCTTGTTTCCGGTCACC+GGG 0.455694 1.2:-51861504 None:intergenic
GTTGGGCCGGAGTATTATGT+TGG 0.456605 1.2:+51861858 MS.gene059007:CDS
TCAAGAGAGGTTGAAGCCTC+AGG 0.464256 1.2:+51861722 MS.gene059007:CDS
CTATTGTTTCGAGTTCGGTT+GGG 0.476955 1.2:+51861841 MS.gene059007:CDS
CGTGGCTCGCCGATGAGTGT+TGG 0.479586 1.2:+51861786 MS.gene059007:CDS
TTGGGCCGGAGTATTATGTT+GGG 0.486241 1.2:+51861859 MS.gene059007:CDS
GGTAACCGTGAAGCGGCATC+CGG 0.494841 1.2:-51861606 None:intergenic
GACAAGGCCATCCCGGTGAC+CGG 0.499250 1.2:+51861493 MS.gene059007:CDS
GTTGAAGCCTCAGGAGGAGA+AGG 0.505715 1.2:+51861731 MS.gene059007:CDS
CCTTGTCGGTTCATGCCGCC+GGG 0.509201 1.2:-51861477 None:intergenic
ATTCGAACCAGCAGCACCAC+CGG 0.515131 1.2:+51861548 MS.gene059007:CDS
AATCGATCGACGATGGGTCA+AGG 0.531008 1.2:+51861450 None:intergenic
CTTGTTTCCGGTCACCGGGA+TGG 0.534444 1.2:-51861500 None:intergenic
GAAGGATTATTTGTTGATGG+AGG 0.541197 1.2:+51861683 MS.gene059007:CDS
CGTAAACAACGCAAACAAAA+GGG 0.541325 1.2:+51861582 MS.gene059007:CDS
ATGAACCGACAAGGCCATCC+CGG 0.552573 1.2:+51861486 MS.gene059007:CDS
ACAACGCAAACAAAAGGGTC+CGG 0.558245 1.2:+51861587 MS.gene059007:CDS
GCCTCAGGAGGAGAAGGCGG+AGG 0.564123 1.2:+51861737 MS.gene059007:CDS
AGGGTCCGGATGCCGCTTCA+CGG 0.566054 1.2:+51861601 MS.gene059007:CDS
TGAGGCTGTTAAAGCTTGAG+AGG 0.566340 1.2:+51861658 MS.gene059007:CDS
TCCAGATTCCCAACACTCAT+CGG 0.570778 1.2:-51861795 None:intergenic
TCCTCCGCCTTCTCCTCCTG+AGG 0.587094 1.2:-51861738 None:intergenic
GATAAGGATCAATTGGAGCC+TGG 0.591343 1.2:+51861897 MS.gene059007:CDS
ACGGTTACCGACGGTGACTC+CGG 0.591399 1.2:+51861620 MS.gene059007:CDS
AAAATCCCAACATAATACTC+CGG 0.602001 1.2:-51861864 None:intergenic
TGCCGCTTCACGGTTACCGA+CGG 0.603500 1.2:+51861611 MS.gene059007:CDS
GTTAAAGCTTGAGAGGGTGA+AGG 0.609846 1.2:+51861665 MS.gene059007:CDS
CACCGTCGGTAACCGTGAAG+CGG 0.611205 1.2:-51861613 None:intergenic
AGTTTGTTGCGTATCAAGAG+AGG 0.615599 1.2:+51861709 MS.gene059007:CDS
ACGATGGGTCAAGGAACTCC+CGG 0.616899 1.2:+51861459 None:intergenic
CCCGGTGACCGGAAACAAGA+CGG 0.618125 1.2:+51861504 MS.gene059007:CDS
GCAGCACCACCGGCGCGTGT+AGG 0.619998 1.2:+51861558 MS.gene059007:CDS
GAAGCCTCAGGAGGAGAAGG+CGG 0.620796 1.2:+51861734 MS.gene059007:CDS
CCCGGCGGCATGAACCGACA+AGG 0.629868 1.2:+51861477 MS.gene059007:CDS
TGCATCAAAATAGCACAACC+AGG 0.642077 1.2:-51861915 None:intergenic
TTACGACCTACACGCGCCGG+TGG 0.648178 1.2:-51861564 None:intergenic
CGGTATCGAAGTGCAAGCTG+AGG 0.651265 1.2:+51861640 MS.gene059007:CDS
TGTTTACGACCTACACGCGC+CGG 0.657461 1.2:-51861567 None:intergenic
TTCGATACCGGAGTCACCGT+CGG 0.658712 1.2:-51861627 None:intergenic
AGAGAGGTTGAAGCCTCAGG+AGG 0.663698 1.2:+51861725 MS.gene059007:CDS
GAGGCTGTTAAAGCTTGAGA+GGG 0.670530 1.2:+51861659 MS.gene059007:CDS
GATGAGTGTTGGGAATCTGG+AGG 0.708876 1.2:+51861797 MS.gene059007:CDS
TCTAAGGTTGATGATCTACG+TGG 0.778964 1.2:+51861768 MS.gene059007:CDS
ATGGGTCAAGGAACTCCCGG+CGG 0.785552 1.2:+51861462 MS.gene059007:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTTTATCTTTTGTTGATA+AGG + chr1.2:51861881-51861900 MS.gene059007:CDS 20.0%
!!! TGCTATTTTGATGCATAATA+AGG + chr1.2:51861923-51861942 MS.gene059007:CDS 25.0%
!!! TTTTGTTGATAAGGATCAAT+TGG + chr1.2:51861890-51861909 MS.gene059007:CDS 25.0%
AAAATCCCAACATAATACTC+CGG - chr1.2:51861867-51861886 None:intergenic 30.0%
CGTAAACAACGCAAACAAAA+GGG + chr1.2:51861582-51861601 MS.gene059007:CDS 35.0%
TCGTAAACAACGCAAACAAA+AGG + chr1.2:51861581-51861600 MS.gene059007:CDS 35.0%
! GAAGGATTATTTGTTGATGG+AGG + chr1.2:51861683-51861702 MS.gene059007:CDS 35.0%
! GGTGAAGGATTATTTGTTGA+TGG + chr1.2:51861680-51861699 MS.gene059007:CDS 35.0%
!! TCATGCTATTGTTTCGAGTT+CGG + chr1.2:51861836-51861855 MS.gene059007:CDS 35.0%
TGCATCAAAATAGCACAACC+AGG - chr1.2:51861918-51861937 None:intergenic 40.0%
!! AGTTTGTTGCGTATCAAGAG+AGG + chr1.2:51861709-51861728 MS.gene059007:CDS 40.0%
!! CTATTGTTTCGAGTTCGGTT+GGG + chr1.2:51861841-51861860 MS.gene059007:CDS 40.0%
!! TCTAAGGTTGATGATCTACG+TGG + chr1.2:51861768-51861787 MS.gene059007:CDS 40.0%
ACAACGCAAACAAAAGGGTC+CGG + chr1.2:51861587-51861606 MS.gene059007:CDS 45.0%
GAGGCTGTTAAAGCTTGAGA+GGG + chr1.2:51861659-51861678 MS.gene059007:CDS 45.0%
GATAAGGATCAATTGGAGCC+TGG + chr1.2:51861897-51861916 MS.gene059007:CDS 45.0%
GTTAAAGCTTGAGAGGGTGA+AGG + chr1.2:51861665-51861684 MS.gene059007:CDS 45.0%
TCCAGATTCCCAACACTCAT+CGG - chr1.2:51861798-51861817 None:intergenic 45.0%
TGAGGCTGTTAAAGCTTGAG+AGG + chr1.2:51861658-51861677 MS.gene059007:CDS 45.0%
! TTGGGCCGGAGTATTATGTT+GGG + chr1.2:51861859-51861878 MS.gene059007:CDS 45.0%
!! GCTATTGTTTCGAGTTCGGT+TGG + chr1.2:51861840-51861859 MS.gene059007:CDS 45.0%
CTCAGCTTGCACTTCGATAC+CGG - chr1.2:51861642-51861661 None:intergenic 50.0%
GGCGGAGGAAGATAGATCTA+AGG + chr1.2:51861752-51861771 MS.gene059007:CDS 50.0%
TCAAGAGAGGTTGAAGCCTC+AGG + chr1.2:51861722-51861741 MS.gene059007:CDS 50.0%
! GTTGGGCCGGAGTATTATGT+TGG + chr1.2:51861858-51861877 MS.gene059007:CDS 50.0%
!! GATGAGTGTTGGGAATCTGG+AGG + chr1.2:51861797-51861816 MS.gene059007:CDS 50.0%
!! TATCGCTGCCGTCTTGTTTC+CGG - chr1.2:51861515-51861534 None:intergenic 50.0%
AGAGAGGTTGAAGCCTCAGG+AGG + chr1.2:51861725-51861744 MS.gene059007:CDS 55.0%
ATGAACCGACAAGGCCATCC+CGG + chr1.2:51861486-51861505 MS.gene059007:CDS 55.0%
ATTCGAACCAGCAGCACCAC+CGG + chr1.2:51861548-51861567 MS.gene059007:CDS 55.0%
GTTGAAGCCTCAGGAGGAGA+AGG + chr1.2:51861731-51861750 MS.gene059007:CDS 55.0%
TGTTTACGACCTACACGCGC+CGG - chr1.2:51861570-51861589 None:intergenic 55.0%
TTCGATACCGGAGTCACCGT+CGG - chr1.2:51861630-51861649 None:intergenic 55.0%
! CGGTATCGAAGTGCAAGCTG+AGG + chr1.2:51861640-51861659 MS.gene059007:CDS 55.0%
!! GCCGATGAGTGTTGGGAATC+TGG + chr1.2:51861794-51861813 MS.gene059007:CDS 55.0%
!! TGTTTCGAGTTCGGTTGGGC+CGG + chr1.2:51861845-51861864 MS.gene059007:CDS 55.0%
ACGGTTACCGACGGTGACTC+CGG + chr1.2:51861620-51861639 MS.gene059007:CDS 60.0%
ATGGGTCAAGGAACTCCCGG+CGG + chr1.2:51861462-51861481 MS.gene059007:CDS 60.0%
CACCGTCGGTAACCGTGAAG+CGG - chr1.2:51861616-51861635 None:intergenic 60.0%
CCCGGTGACCGGAAACAAGA+CGG + chr1.2:51861504-51861523 MS.gene059007:CDS 60.0%
CTTGTTTCCGGTCACCGGGA+TGG - chr1.2:51861503-51861522 None:intergenic 60.0%
GAAGCCTCAGGAGGAGAAGG+CGG + chr1.2:51861734-51861753 MS.gene059007:CDS 60.0%
GGTAACCGTGAAGCGGCATC+CGG - chr1.2:51861609-51861628 None:intergenic 60.0%
GTGGCTCGCCGATGAGTGTT+GGG + chr1.2:51861787-51861806 MS.gene059007:CDS 60.0%
! TGCCGCTTCACGGTTACCGA+CGG + chr1.2:51861611-51861630 MS.gene059007:CDS 60.0%
!! CCGTCTTGTTTCCGGTCACC+GGG - chr1.2:51861507-51861526 None:intergenic 60.0%
!! GCCGTCTTGTTTCCGGTCAC+CGG - chr1.2:51861508-51861527 None:intergenic 60.0%
CGTGGCTCGCCGATGAGTGT+TGG + chr1.2:51861786-51861805 MS.gene059007:CDS 65.0%
GACAAGGCCATCCCGGTGAC+CGG + chr1.2:51861493-51861512 MS.gene059007:CDS 65.0%
TCCTCCGCCTTCTCCTCCTG+AGG - chr1.2:51861741-51861760 None:intergenic 65.0%
TTACGACCTACACGCGCCGG+TGG - chr1.2:51861567-51861586 None:intergenic 65.0%
! AGGGTCCGGATGCCGCTTCA+CGG + chr1.2:51861601-51861620 MS.gene059007:CDS 65.0%
!! CCTTGTCGGTTCATGCCGCC+GGG - chr1.2:51861480-51861499 None:intergenic 65.0%
!! GCCTTGTCGGTTCATGCCGC+CGG - chr1.2:51861481-51861500 None:intergenic 65.0%
!! GGTCACCGGGATGGCCTTGT+CGG - chr1.2:51861494-51861513 None:intergenic 65.0%
CCCGGCGGCATGAACCGACA+AGG + chr1.2:51861477-51861496 MS.gene059007:CDS 70.0%
GCCTCAGGAGGAGAAGGCGG+AGG + chr1.2:51861737-51861756 MS.gene059007:CDS 70.0%
GCAGCACCACCGGCGCGTGT+AGG + chr1.2:51861558-51861577 MS.gene059007:CDS 75.0%
!! ACACGCGCCGGTGGTGCTGC+TGG - chr1.2:51861558-51861577 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 51861462 51861962 51861462 ID=MS.gene059007
chr1.2 mRNA 51861462 51861962 51861462 ID=MS.gene059007.t1;Parent=MS.gene059007
chr1.2 exon 51861462 51861962 51861462 ID=MS.gene059007.t1.exon1;Parent=MS.gene059007.t1
chr1.2 CDS 51861462 51861962 51861462 ID=cds.MS.gene059007.t1;Parent=MS.gene059007.t1
Gene Sequence

>MS.gene059007

ATGGGTCAAGGAACTCCCGGCGGCATGAACCGACAAGGCCATCCCGGTGACCGGAAACAAGACGGCAGCGATAAAAAAGAAAAGAAATTCGAACCAGCAGCACCACCGGCGCGTGTAGGTCGTAAACAACGCAAACAAAAGGGTCCGGATGCCGCTTCACGGTTACCGACGGTGACTCCGGTATCGAAGTGCAAGCTGAGGCTGTTAAAGCTTGAGAGGGTGAAGGATTATTTGTTGATGGAGGAAGAGTTTGTTGCGTATCAAGAGAGGTTGAAGCCTCAGGAGGAGAAGGCGGAGGAAGATAGATCTAAGGTTGATGATCTACGTGGCTCGCCGATGAGTGTTGGGAATCTGGAGGAATTGATTGATGAGAATCATGCTATTGTTTCGAGTTCGGTTGGGCCGGAGTATTATGTTGGGATTTTATCTTTTGTTGATAAGGATCAATTGGAGCCTGGTTGTGCTATTTTGATGCATAATAAGGTATTTCTTGATTGTTGA

Protein sequence

>MS.gene059007.t1

MGQGTPGGMNRQGHPGDRKQDGSDKKEKKFEPAAPPARVGRKQRKQKGPDAASRLPTVTPVSKCKLRLLKLERVKDYLLMEEEFVAYQERLKPQEEKAEEDRSKVDDLRGSPMSVGNLEELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCAILMHNKVFLDC