Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28701.t1 | RHN61599.1 | 97.4 | 151 | 4 | 0 | 1 | 151 | 261 | 411 | 6.80E-74 | 286.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28701.t1 | Q9FFY6 | 63.5 | 148 | 52 | 1 | 2 | 147 | 220 | 367 | 6.3e-44 | 178.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28701.t1 | A0A396I7K1 | 97.4 | 151 | 4 | 0 | 1 | 151 | 261 | 411 | 4.9e-74 | 286.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene28701 | 0.803992 | 2.67E-49 | -1.69E-46 |
| MS.gene050414 | MS.gene28701 | 0.803858 | 2.85E-49 | -1.69E-46 |
| MS.gene060259 | MS.gene28701 | 0.805229 | 1.48E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28701.t1 | MTR_4g075250 | 96.689 | 151 | 5 | 0 | 1 | 151 | 261 | 411 | 3.18e-107 | 311 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28701.t1 | AT3G12400 | 63.448 | 145 | 52 | 1 | 4 | 147 | 253 | 397 | 6.01e-60 | 190 |
| MS.gene28701.t1 | AT5G13860 | 62.838 | 148 | 53 | 1 | 2 | 147 | 220 | 367 | 2.43e-59 | 188 |
| MS.gene28701.t1 | AT3G12400 | 63.830 | 141 | 50 | 1 | 4 | 143 | 253 | 393 | 2.06e-58 | 186 |
| MS.gene28701.t1 | AT3G12400 | 63.830 | 141 | 50 | 1 | 4 | 143 | 253 | 393 | 4.66e-58 | 186 |
Find 44 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTTAGATAAGGGTGTTC+AGG | 0.272232 | 4.2:+33566348 | MS.gene28701:CDS |
| GGAGAATCAAGGGAAGAAAA+TGG | 0.279130 | 4.2:+33566222 | MS.gene28701:CDS |
| GCAGGCTCAGGTTGCGAATA+TGG | 0.279429 | 4.2:+33566474 | MS.gene28701:CDS |
| TAGATAAGGGTGTTCAGGTT+GGG | 0.287353 | 4.2:+33566353 | MS.gene28701:CDS |
| AGGGAAGAAAATGGGGAGTT+TGG | 0.299911 | 4.2:+33566231 | MS.gene28701:CDS |
| TGATTGTACTTCTGCTGATT+TGG | 0.313474 | 4.2:+33566306 | MS.gene28701:CDS |
| TTTGATGAATACCGATGTTT+TGG | 0.314875 | 4.2:+33566186 | MS.gene28701:CDS |
| GAGAATCAAGGGAAGAAAAT+GGG | 0.338918 | 4.2:+33566223 | MS.gene28701:CDS |
| AGTTAGAGCTGCTCAGTTGC+AGG | 0.360251 | 4.2:+33566456 | MS.gene28701:CDS |
| ACGCTTTATGCTTTAGATAA+GGG | 0.364897 | 4.2:+33566340 | MS.gene28701:CDS |
| GGTGAAATGGAGGGATTGTT+TGG | 0.368047 | 4.2:+33566064 | None:intergenic |
| GCAAGATGAGATGGAGGGTT+TGG | 0.370726 | 4.2:+33566144 | MS.gene28701:CDS |
| AGCTGCTCAGTTGCAGGCTC+AGG | 0.393395 | 4.2:+33566462 | MS.gene28701:CDS |
| AGATAAGGGTGTTCAGGTTG+GGG | 0.397945 | 4.2:+33566354 | MS.gene28701:CDS |
| TACGCTTTATGCTTTAGATA+AGG | 0.408278 | 4.2:+33566339 | MS.gene28701:CDS |
| GCTGCTAGGAATCATCATTA+TGG | 0.423124 | 4.2:+33566496 | MS.gene28701:CDS |
| GGTATTGAAGCAGAGGGAGG+AGG | 0.425612 | 4.2:+33566096 | MS.gene28701:CDS |
| TACCGTTTGATCAGTATTTG+AGG | 0.441585 | 4.2:+33566380 | MS.gene28701:CDS |
| CTCCTCAAATACTGATCAAA+CGG | 0.461994 | 4.2:-33566382 | None:intergenic |
| GGAGATGCAAGATGAGATGG+AGG | 0.478519 | 4.2:+33566138 | MS.gene28701:CDS |
| TTAGATAAGGGTGTTCAGGT+TGG | 0.491802 | 4.2:+33566352 | MS.gene28701:CDS |
| GAGGGATTGTTTGGTTTGCA+AGG | 0.492439 | 4.2:+33566073 | MS.gene28701:CDS |
| GGGATTGTTTGGTTTGCAAG+GGG | 0.495332 | 4.2:+33566075 | MS.gene28701:CDS |
| GGTTGGTTGAGGGAGAATCA+AGG | 0.499129 | 4.2:+33566211 | MS.gene28701:CDS |
| TGCAAGGGGTATTGAAGCAG+AGG | 0.500833 | 4.2:+33566089 | MS.gene28701:CDS |
| GTTGGTTGAGGGAGAATCAA+GGG | 0.518369 | 4.2:+33566212 | MS.gene28701:CDS |
| AGGGATTGTTTGGTTTGCAA+GGG | 0.525158 | 4.2:+33566074 | MS.gene28701:CDS |
| GAGTGTGAGAGCGTTGTCGA+GGG | 0.528786 | 4.2:+33566402 | MS.gene28701:CDS |
| GAAGGAGATGCAAGATGAGA+TGG | 0.537662 | 4.2:+33566135 | MS.gene28701:CDS |
| AGAATCAAGGGAAGAAAATG+GGG | 0.538001 | 4.2:+33566224 | MS.gene28701:CDS |
| TTTGGAGCAGCAGTTGCAGA+TGG | 0.557427 | 4.2:+33566162 | MS.gene28701:CDS |
| GCAGTTCTTCCACCGTGCTA+CGG | 0.559208 | 4.2:+33566426 | MS.gene28701:CDS |
| GGGGAGTTTGGAGAATGTTG+AGG | 0.560828 | 4.2:+33566243 | MS.gene28701:CDS |
| ACTAGAGAAGGTGAAATGGA+GGG | 0.566363 | 4.2:+33566055 | None:intergenic |
| GGAGTGTGAGAGCGTTGTCG+AGG | 0.576375 | 4.2:+33566401 | MS.gene28701:CDS |
| GAGTTTGGAGAATGTTGAGG+AGG | 0.585981 | 4.2:+33566246 | MS.gene28701:CDS |
| AGGTTGCGAATATGGCTGCT+AGG | 0.587435 | 4.2:+33566482 | MS.gene28701:CDS |
| AATCATCATTATGGTAGCTG+AGG | 0.620092 | 4.2:+33566505 | MS.gene28701:CDS |
| TAACTTTAGCAGCCGTAGCA+CGG | 0.627369 | 4.2:-33566438 | None:intergenic |
| AGGGGTATTGAAGCAGAGGG+AGG | 0.628332 | 4.2:+33566093 | MS.gene28701:CDS |
| CAACCAACCCTCCAAAACAT+CGG | 0.653851 | 4.2:-33566197 | None:intergenic |
| GAGATGCAAGATGAGATGGA+GGG | 0.654104 | 4.2:+33566139 | MS.gene28701:CDS |
| GCAAGGGGTATTGAAGCAGA+GGG | 0.664131 | 4.2:+33566090 | MS.gene28701:CDS |
| CTTTAGCAGCCGTAGCACGG+TGG | 0.725711 | 4.2:-33566435 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACGCTTTATGCTTTAGATAA+GGG | + | chr4.2:33566340-33566359 | MS.gene28701:CDS | 30.0% |
| ! | TACGCTTTATGCTTTAGATA+AGG | + | chr4.2:33566339-33566358 | MS.gene28701:CDS | 30.0% |
| ! | TTTGATGAATACCGATGTTT+TGG | + | chr4.2:33566186-33566205 | MS.gene28701:CDS | 30.0% |
| AGAATCAAGGGAAGAAAATG+GGG | + | chr4.2:33566224-33566243 | MS.gene28701:CDS | 35.0% | |
| CTCCTCAAATACTGATCAAA+CGG | - | chr4.2:33566385-33566404 | None:intergenic | 35.0% | |
| GAGAATCAAGGGAAGAAAAT+GGG | + | chr4.2:33566223-33566242 | MS.gene28701:CDS | 35.0% | |
| TACCGTTTGATCAGTATTTG+AGG | + | chr4.2:33566380-33566399 | MS.gene28701:CDS | 35.0% | |
| ! | TGATTGTACTTCTGCTGATT+TGG | + | chr4.2:33566306-33566325 | MS.gene28701:CDS | 35.0% |
| !! | ATGAATACCGATGTTTTGGA+GGG | + | chr4.2:33566190-33566209 | MS.gene28701:CDS | 35.0% |
| !!! | GGTTTTGAATAATGGTGTGA+AGG | + | chr4.2:33566117-33566136 | MS.gene28701:CDS | 35.0% |
| GCTGCTAGGAATCATCATTA+TGG | + | chr4.2:33566496-33566515 | MS.gene28701:CDS | 40.0% | |
| GGAGAATCAAGGGAAGAAAA+TGG | + | chr4.2:33566222-33566241 | MS.gene28701:CDS | 40.0% | |
| !! | AGGGATTGTTTGGTTTGCAA+GGG | + | chr4.2:33566074-33566093 | MS.gene28701:CDS | 40.0% |
| !! | GATGAATACCGATGTTTTGG+AGG | + | chr4.2:33566189-33566208 | MS.gene28701:CDS | 40.0% |
| !! | TAGATAAGGGTGTTCAGGTT+GGG | + | chr4.2:33566353-33566372 | MS.gene28701:CDS | 40.0% |
| !! | TGCTTTAGATAAGGGTGTTC+AGG | + | chr4.2:33566348-33566367 | MS.gene28701:CDS | 40.0% |
| !! | TTAGATAAGGGTGTTCAGGT+TGG | + | chr4.2:33566352-33566371 | MS.gene28701:CDS | 40.0% |
| !!! | AGGGAGGAGGTTTTGAATAA+TGG | + | chr4.2:33566109-33566128 | MS.gene28701:CDS | 40.0% |
| AGGGAAGAAAATGGGGAGTT+TGG | + | chr4.2:33566231-33566250 | MS.gene28701:CDS | 45.0% | |
| CAACCAACCCTCCAAAACAT+CGG | - | chr4.2:33566200-33566219 | None:intergenic | 45.0% | |
| GAAGGAGATGCAAGATGAGA+TGG | + | chr4.2:33566135-33566154 | MS.gene28701:CDS | 45.0% | |
| GAGATGCAAGATGAGATGGA+GGG | + | chr4.2:33566139-33566158 | MS.gene28701:CDS | 45.0% | |
| GAGTTTGGAGAATGTTGAGG+AGG | + | chr4.2:33566246-33566265 | MS.gene28701:CDS | 45.0% | |
| GTTGGTTGAGGGAGAATCAA+GGG | + | chr4.2:33566212-33566231 | MS.gene28701:CDS | 45.0% | |
| TAACTTTAGCAGCCGTAGCA+CGG | - | chr4.2:33566441-33566460 | None:intergenic | 45.0% | |
| !! | AGATAAGGGTGTTCAGGTTG+GGG | + | chr4.2:33566354-33566373 | MS.gene28701:CDS | 45.0% |
| !! | GAGGGATTGTTTGGTTTGCA+AGG | + | chr4.2:33566073-33566092 | MS.gene28701:CDS | 45.0% |
| !! | GGGATTGTTTGGTTTGCAAG+GGG | + | chr4.2:33566075-33566094 | MS.gene28701:CDS | 45.0% |
| !!! | ATACCGATGTTTTGGAGGGT+TGG | + | chr4.2:33566194-33566213 | MS.gene28701:CDS | 45.0% |
| !!! | ATGTTTTGGAGGGTTGGTTG+AGG | + | chr4.2:33566200-33566219 | MS.gene28701:CDS | 45.0% |
| !!! | TGTTTTGGAGGGTTGGTTGA+GGG | + | chr4.2:33566201-33566220 | MS.gene28701:CDS | 45.0% |
| AGGTTGCGAATATGGCTGCT+AGG | + | chr4.2:33566482-33566501 | MS.gene28701:CDS | 50.0% | |
| AGTTAGAGCTGCTCAGTTGC+AGG | + | chr4.2:33566456-33566475 | MS.gene28701:CDS | 50.0% | |
| GCAAGATGAGATGGAGGGTT+TGG | + | chr4.2:33566144-33566163 | MS.gene28701:CDS | 50.0% | |
| GGAGATGCAAGATGAGATGG+AGG | + | chr4.2:33566138-33566157 | MS.gene28701:CDS | 50.0% | |
| GGGGAGTTTGGAGAATGTTG+AGG | + | chr4.2:33566243-33566262 | MS.gene28701:CDS | 50.0% | |
| ! | GGTTGGTTGAGGGAGAATCA+AGG | + | chr4.2:33566211-33566230 | MS.gene28701:CDS | 50.0% |
| ! | TTTGGAGCAGCAGTTGCAGA+TGG | + | chr4.2:33566162-33566181 | MS.gene28701:CDS | 50.0% |
| !! | GCAAGGGGTATTGAAGCAGA+GGG | + | chr4.2:33566090-33566109 | MS.gene28701:CDS | 50.0% |
| !! | TGAGGAGGCTTTTGAGTGTG+TGG | + | chr4.2:33566261-33566280 | MS.gene28701:CDS | 50.0% |
| !! | TGCAAGGGGTATTGAAGCAG+AGG | + | chr4.2:33566089-33566108 | MS.gene28701:CDS | 50.0% |
| GCAGTTCTTCCACCGTGCTA+CGG | + | chr4.2:33566426-33566445 | MS.gene28701:CDS | 55.0% | |
| ! | GCAGGCTCAGGTTGCGAATA+TGG | + | chr4.2:33566474-33566493 | MS.gene28701:CDS | 55.0% |
| ! | GGTATTGAAGCAGAGGGAGG+AGG | + | chr4.2:33566096-33566115 | MS.gene28701:CDS | 55.0% |
| !! | AGGGGTATTGAAGCAGAGGG+AGG | + | chr4.2:33566093-33566112 | MS.gene28701:CDS | 55.0% |
| !! | GAGTGTGAGAGCGTTGTCGA+GGG | + | chr4.2:33566402-33566421 | MS.gene28701:CDS | 55.0% |
| AGCTGCTCAGTTGCAGGCTC+AGG | + | chr4.2:33566462-33566481 | MS.gene28701:CDS | 60.0% | |
| CTTTAGCAGCCGTAGCACGG+TGG | - | chr4.2:33566438-33566457 | None:intergenic | 60.0% | |
| !! | GGAGTGTGAGAGCGTTGTCG+AGG | + | chr4.2:33566401-33566420 | MS.gene28701:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 33566070 | 33566525 | 33566070 | ID=MS.gene28701 |
| chr4.2 | mRNA | 33566070 | 33566525 | 33566070 | ID=MS.gene28701.t1;Parent=MS.gene28701 |
| chr4.2 | exon | 33566070 | 33566525 | 33566070 | ID=MS.gene28701.t1.exon1;Parent=MS.gene28701.t1 |
| chr4.2 | CDS | 33566070 | 33566525 | 33566070 | ID=cds.MS.gene28701.t1;Parent=MS.gene28701.t1 |
| Gene Sequence |
| Protein sequence |
