Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29485.t1 | XP_003616408.2 | 89.9 | 159 | 2 | 2 | 1 | 145 | 1 | 159 | 1.00E-71 | 279.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29485.t1 | A2WWV5 | 40.1 | 152 | 76 | 2 | 4 | 140 | 5 | 156 | 1.0e-27 | 124.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29485.t1 | G7KBR3 | 89.9 | 159 | 2 | 2 | 1 | 145 | 1 | 159 | 7.5e-72 | 279.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051493 | MS.gene29485 | 0.814828 | 1.26E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29485.t1 | MTR_5g079890 | 89.937 | 159 | 2 | 2 | 1 | 145 | 1 | 159 | 2.87e-100 | 284 |
| MS.gene29485.t1 | MTR_5g079890 | 88.489 | 139 | 2 | 2 | 1 | 125 | 1 | 139 | 1.59e-84 | 244 |
| MS.gene29485.t1 | MTR_5g079880 | 57.419 | 155 | 51 | 3 | 1 | 142 | 1 | 153 | 1.40e-53 | 166 |
| MS.gene29485.t1 | MTR_8g032320 | 38.854 | 157 | 68 | 6 | 1 | 141 | 1 | 145 | 1.87e-27 | 99.8 |
| MS.gene29485.t1 | MTR_8g032300 | 36.306 | 157 | 72 | 6 | 1 | 141 | 1 | 145 | 2.53e-24 | 92.0 |
| MS.gene29485.t1 | MTR_1g008950 | 34.307 | 137 | 65 | 5 | 1 | 124 | 1 | 125 | 1.65e-19 | 79.0 |
| MS.gene29485.t1 | MTR_8g030870 | 31.915 | 141 | 81 | 3 | 1 | 127 | 1 | 140 | 5.64e-13 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29485.t1 | AT3G55470 | 40.523 | 153 | 77 | 2 | 1 | 140 | 1 | 152 | 9.98e-36 | 121 |
| MS.gene29485.t1 | AT3G55470 | 40.260 | 154 | 77 | 2 | 1 | 140 | 1 | 153 | 1.66e-35 | 120 |
| MS.gene29485.t1 | AT1G63220 | 35.849 | 159 | 73 | 7 | 1 | 142 | 1 | 147 | 6.94e-20 | 80.5 |
| MS.gene29485.t1 | AT1G63220 | 34.932 | 146 | 78 | 6 | 1 | 142 | 1 | 133 | 3.22e-15 | 68.2 |
| MS.gene29485.t1 | AT4G00467 | 29.412 | 136 | 78 | 3 | 1 | 121 | 1 | 133 | 1.83e-13 | 64.7 |
| MS.gene29485.t1 | AT4G00467 | 29.412 | 136 | 78 | 3 | 1 | 121 | 1 | 133 | 3.03e-13 | 64.3 |
Find 24 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCTATTGTAAAGATTTAT+TGG | 0.151708 | 5.3:+64572493 | MS.gene29485:CDS |
| CTCAATAGCACTTGTTTCTT+TGG | 0.198258 | 5.3:+64572065 | MS.gene29485:CDS |
| TTGGCTCAAGGTGTTCAAAA+TGG | 0.301303 | 5.3:+64572512 | MS.gene29485:CDS |
| AGATATGGCAATTGGGATAA+TGG | 0.338735 | 5.3:+64572016 | None:intergenic |
| CAAAGTCATTCTCAAAATAA+TGG | 0.344213 | 5.3:+64572318 | MS.gene29485:CDS |
| ACACTCAAAGATATGGCAAT+TGG | 0.382620 | 5.3:+64572008 | None:intergenic |
| CACTCAAAGATATGGCAATT+GGG | 0.464148 | 5.3:+64572009 | None:intergenic |
| CAATCTCTCCACGATAAGAT+TGG | 0.475600 | 5.3:-64572580 | None:intergenic |
| TTGATATTGTATCAAAACAT+AGG | 0.476782 | 5.3:-64572191 | None:intergenic |
| CAAAGAAACAAGTGCTATTG+AGG | 0.483426 | 5.3:-64572064 | None:intergenic |
| GTACATCTTGTGAAAGCAAA+AGG | 0.483814 | 5.3:+64572041 | MS.gene29485:CDS |
| CCACACGGCTCCTATGCTCT+TGG | 0.487013 | 5.3:-64572216 | None:intergenic |
| TGTAAAGATTTATTGGCTCA+AGG | 0.515900 | 5.3:+64572500 | MS.gene29485:CDS |
| AATTCATTCACAGGTGGAAG+AGG | 0.517863 | 5.3:+64572770 | MS.gene29485:intron |
| ATATTAATATATAGGTGGAA+TGG | 0.546882 | 5.3:+64572162 | MS.gene29485:intron |
| TTGAAGAAGACATAGGAGGA+TGG | 0.550578 | 5.3:+64572799 | MS.gene29485:CDS |
| TGATATTGTATCAAAACATA+GGG | 0.550960 | 5.3:-64572190 | None:intergenic |
| GAATTAATTGAAGAAGACAT+AGG | 0.564791 | 5.3:+64572792 | MS.gene29485:CDS |
| AATATCAAGGCCAAGAGCAT+AGG | 0.578423 | 5.3:+64572206 | MS.gene29485:CDS |
| CGAACAACTCTATACTTGAG+AGG | 0.601631 | 5.3:-64572551 | None:intergenic |
| TTAATTGAAGAAGACATAGG+AGG | 0.621683 | 5.3:+64572795 | MS.gene29485:CDS |
| ATGTTCAAAGTAGAAAACAA+TGG | 0.628110 | 5.3:+64572280 | MS.gene29485:CDS |
| CGTGCAGACCAATCTTATCG+TGG | 0.690565 | 5.3:+64572572 | MS.gene29485:CDS |
| ACAACTCTATACTTGAGAGG+TGG | 0.693150 | 5.3:-64572548 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATAAATATATGATACA+AGG | - | chr5.3:64572132-64572151 | None:intergenic | 10.0% |
| !! | ATAATAAATATATGATACAA+GGG | - | chr5.3:64572131-64572150 | None:intergenic | 10.0% |
| !! | ATATATTAATTAATTACCTT+TGG | - | chr5.3:64572626-64572645 | None:intergenic | 10.0% |
| !! | TCAAAAAAAATATATAGTTA+TGG | + | chr5.3:64572700-64572719 | MS.gene29485:intron | 10.0% |
| !!! | AACTATATATTTTTTTTGAA+GGG | - | chr5.3:64572699-64572718 | None:intergenic | 10.0% |
| !!! | TAACTATATATTTTTTTTGA+AGG | - | chr5.3:64572700-64572719 | None:intergenic | 10.0% |
| !!! | TATTTCATTTTTGTTAATTA+AGG | + | chr5.3:64572649-64572668 | MS.gene29485:intron | 10.0% |
| !! | AATCTATTGTAAAGATTTAT+TGG | + | chr5.3:64572493-64572512 | MS.gene29485:CDS | 15.0% |
| !! | TTACTATATTAATATATAGG+TGG | + | chr5.3:64572157-64572176 | MS.gene29485:intron | 15.0% |
| !! | AAAAATGAGACATTATTGTT+AGG | - | chr5.3:64572416-64572435 | None:intergenic | 20.0% |
| !! | ATATTAATATATAGGTGGAA+TGG | + | chr5.3:64572162-64572181 | MS.gene29485:intron | 20.0% |
| !! | CATGTAACAATATATAGTTA+TGG | + | chr5.3:64572672-64572691 | MS.gene29485:intron | 20.0% |
| !! | TATTGTTAGGAATATATATG+TGG | - | chr5.3:64572403-64572422 | None:intergenic | 20.0% |
| !! | TGATATTGTATCAAAACATA+GGG | - | chr5.3:64572193-64572212 | None:intergenic | 20.0% |
| !! | TTGATATTGTATCAAAACAT+AGG | - | chr5.3:64572194-64572213 | None:intergenic | 20.0% |
| !! | TTTACAATAGATTCTGTTTA+AGG | - | chr5.3:64572486-64572505 | None:intergenic | 20.0% |
| !!! | AGTTGATTATTTTTCTTTGT+TGG | + | chr5.3:64572747-64572766 | MS.gene29485:intron | 20.0% |
| !!! | TATGTTTTGATACAATATCA+AGG | + | chr5.3:64572193-64572212 | MS.gene29485:CDS | 20.0% |
| ! | ATGTTCAAAGTAGAAAACAA+TGG | + | chr5.3:64572280-64572299 | MS.gene29485:CDS | 25.0% |
| ! | CAAAGTCATTCTCAAAATAA+TGG | + | chr5.3:64572318-64572337 | MS.gene29485:CDS | 25.0% |
| ! | GAATTAATTGAAGAAGACAT+AGG | + | chr5.3:64572792-64572811 | MS.gene29485:CDS | 25.0% |
| ! | GGCAAAATATATGAATGTAA+GGG | - | chr5.3:64572110-64572129 | None:intergenic | 25.0% |
| GGGCAAAATATATGAATGTA+AGG | - | chr5.3:64572111-64572130 | None:intergenic | 30.0% | |
| GTTAGGAATATATATGTGGA+AGG | - | chr5.3:64572399-64572418 | None:intergenic | 30.0% | |
| TAGAGGCAAAAATCATGTAT+GGG | + | chr5.3:64572246-64572265 | MS.gene29485:intron | 30.0% | |
| TTAATTGAAGAAGACATAGG+AGG | + | chr5.3:64572795-64572814 | MS.gene29485:CDS | 30.0% | |
| !! | TGCTATTACTTTTACTCCAA+AGG | + | chr5.3:64572607-64572626 | MS.gene29485:CDS | 30.0% |
| !! | TGTAAAGATTTATTGGCTCA+AGG | + | chr5.3:64572500-64572519 | MS.gene29485:CDS | 30.0% |
| CTTTGTTGGAATTCATTCAC+AGG | + | chr5.3:64572761-64572780 | MS.gene29485:intron | 35.0% | |
| GTACATCTTGTGAAAGCAAA+AGG | + | chr5.3:64572041-64572060 | MS.gene29485:CDS | 35.0% | |
| GTAGAGGCAAAAATCATGTA+TGG | + | chr5.3:64572245-64572264 | MS.gene29485:intron | 35.0% | |
| ! | CAAAGAAACAAGTGCTATTG+AGG | - | chr5.3:64572067-64572086 | None:intergenic | 35.0% |
| ! | CTCAATAGCACTTGTTTCTT+TGG | + | chr5.3:64572065-64572084 | MS.gene29485:CDS | 35.0% |
| ! | TTTTCTGCTGATGACTTCAT+TGG | + | chr5.3:64572352-64572371 | MS.gene29485:CDS | 35.0% |
| AATTCATTCACAGGTGGAAG+AGG | + | chr5.3:64572770-64572789 | MS.gene29485:intron | 40.0% | |
| ACAACTCTATACTTGAGAGG+TGG | - | chr5.3:64572551-64572570 | None:intergenic | 40.0% | |
| CAATCTCTCCACGATAAGAT+TGG | - | chr5.3:64572583-64572602 | None:intergenic | 40.0% | |
| CGAACAACTCTATACTTGAG+AGG | - | chr5.3:64572554-64572573 | None:intergenic | 40.0% | |
| TGTTGGAATTCATTCACAGG+TGG | + | chr5.3:64572764-64572783 | MS.gene29485:intron | 40.0% | |
| TTGAAGAAGACATAGGAGGA+TGG | + | chr5.3:64572799-64572818 | MS.gene29485:CDS | 40.0% | |
| ! | AATATCAAGGCCAAGAGCAT+AGG | + | chr5.3:64572206-64572225 | MS.gene29485:CDS | 40.0% |
| ! | TTGGCTCAAGGTGTTCAAAA+TGG | + | chr5.3:64572512-64572531 | MS.gene29485:CDS | 40.0% |
| !! | TGCCTCTACTTTTAGCCACA+CGG | - | chr5.3:64572234-64572253 | None:intergenic | 45.0% |
| !! | TGGAAGGCATTAGTCTTACG+TGG | - | chr5.3:64572383-64572402 | None:intergenic | 45.0% |
| !! | TAATTACTATATTAATATAT+AGG | + | chr5.3:64572154-64572173 | MS.gene29485:intron | 5.0% |
| AGCCGTGTGGCTAAAAGTAG+AGG | + | chr5.3:64572229-64572248 | MS.gene29485:intron | 50.0% | |
| CGTGCAGACCAATCTTATCG+TGG | + | chr5.3:64572572-64572591 | MS.gene29485:CDS | 50.0% | |
| CCACACGGCTCCTATGCTCT+TGG | - | chr5.3:64572219-64572238 | None:intergenic | 60.0% | |
| ! | CCAAGAGCATAGGAGCCGTG+TGG | + | chr5.3:64572216-64572235 | MS.gene29485:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 64572020 | 64572848 | 64572020 | ID=MS.gene29485 |
| chr5.3 | mRNA | 64572020 | 64572848 | 64572020 | ID=MS.gene29485.t1;Parent=MS.gene29485 |
| chr5.3 | exon | 64572020 | 64572086 | 64572020 | ID=MS.gene29485.t1.exon1;Parent=MS.gene29485.t1 |
| chr5.3 | CDS | 64572020 | 64572086 | 64572020 | ID=cds.MS.gene29485.t1;Parent=MS.gene29485.t1 |
| chr5.3 | exon | 64572176 | 64572234 | 64572176 | ID=MS.gene29485.t1.exon2;Parent=MS.gene29485.t1 |
| chr5.3 | CDS | 64572176 | 64572234 | 64572176 | ID=cds.MS.gene29485.t1;Parent=MS.gene29485.t1 |
| chr5.3 | exon | 64572274 | 64572383 | 64572274 | ID=MS.gene29485.t1.exon3;Parent=MS.gene29485.t1 |
| chr5.3 | CDS | 64572274 | 64572383 | 64572274 | ID=cds.MS.gene29485.t1;Parent=MS.gene29485.t1 |
| chr5.3 | exon | 64572493 | 64572628 | 64572493 | ID=MS.gene29485.t1.exon4;Parent=MS.gene29485.t1 |
| chr5.3 | CDS | 64572493 | 64572628 | 64572493 | ID=cds.MS.gene29485.t1;Parent=MS.gene29485.t1 |
| chr5.3 | exon | 64572783 | 64572848 | 64572783 | ID=MS.gene29485.t1.exon5;Parent=MS.gene29485.t1 |
| chr5.3 | CDS | 64572783 | 64572848 | 64572783 | ID=cds.MS.gene29485.t1;Parent=MS.gene29485.t1 |
| Gene Sequence |
| Protein sequence |
