Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29487.t1 | XP_003616410.1 | 97.1 | 1127 | 32 | 1 | 1 | 1127 | 1 | 1126 | 0.00E+00 | 2045 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29487.t1 | Q9M2T1 | 59.0 | 1003 | 381 | 10 | 129 | 1122 | 1 | 982 | 3.5e-304 | 1045.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29487.t1 | G7KBR5 | 97.1 | 1127 | 32 | 1 | 1 | 1127 | 1 | 1126 | 0.0e+00 | 2045.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene011991 | MS.gene29487 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29487.t1 | MTR_5g079920 | 97.427 | 1127 | 28 | 1 | 1 | 1127 | 1 | 1126 | 0.0 | 2256 |
| MS.gene29487.t1 | MTR_4g076210 | 35.981 | 214 | 124 | 3 | 41 | 245 | 24 | 233 | 4.24e-29 | 126 |
| MS.gene29487.t1 | MTR_4g076210 | 30.000 | 330 | 200 | 7 | 322 | 640 | 228 | 537 | 7.70e-28 | 122 |
| MS.gene29487.t1 | MTR_3g109922 | 26.415 | 212 | 142 | 2 | 46 | 243 | 42 | 253 | 4.03e-17 | 87.4 |
| MS.gene29487.t1 | MTR_3g109922 | 25.828 | 302 | 193 | 13 | 347 | 638 | 275 | 555 | 9.51e-14 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29487.t1 | AT3G55480 | 61.905 | 1134 | 396 | 13 | 1 | 1122 | 1 | 1110 | 0.0 | 1362 |
| MS.gene29487.t1 | AT3G55480 | 58.748 | 1006 | 379 | 13 | 129 | 1122 | 1 | 982 | 0.0 | 1134 |
| MS.gene29487.t1 | AT4G23460 | 36.493 | 211 | 121 | 3 | 41 | 242 | 24 | 230 | 1.72e-28 | 124 |
| MS.gene29487.t1 | AT4G23460 | 31.132 | 318 | 186 | 8 | 335 | 640 | 241 | 537 | 2.60e-28 | 123 |
| MS.gene29487.t1 | AT4G11380 | 31.132 | 318 | 186 | 8 | 335 | 640 | 241 | 537 | 2.03e-28 | 124 |
| MS.gene29487.t1 | AT4G11380 | 35.981 | 214 | 124 | 3 | 41 | 245 | 24 | 233 | 2.80e-26 | 117 |
| MS.gene29487.t1 | AT4G23460 | 31.132 | 318 | 186 | 8 | 335 | 640 | 147 | 443 | 3.05e-28 | 123 |
| MS.gene29487.t1 | AT4G11380 | 31.132 | 318 | 186 | 8 | 335 | 640 | 263 | 559 | 3.13e-28 | 123 |
| MS.gene29487.t1 | AT4G11380 | 36.652 | 221 | 126 | 4 | 34 | 245 | 40 | 255 | 2.76e-26 | 117 |
| MS.gene29487.t1 | AT5G11490 | 27.230 | 213 | 139 | 4 | 46 | 243 | 41 | 252 | 9.47e-17 | 86.3 |
| MS.gene29487.t1 | AT5G11490 | 25.846 | 325 | 206 | 13 | 347 | 661 | 274 | 573 | 2.27e-13 | 75.5 |
| MS.gene29487.t1 | AT5G11490 | 27.230 | 213 | 139 | 4 | 46 | 243 | 41 | 252 | 1.08e-16 | 86.3 |
| MS.gene29487.t1 | AT5G11490 | 25.846 | 325 | 206 | 13 | 347 | 661 | 274 | 573 | 2.63e-13 | 75.1 |
| MS.gene29487.t1 | AT5G11490 | 28.283 | 198 | 122 | 5 | 63 | 243 | 4 | 198 | 3.49e-15 | 81.3 |
| MS.gene29487.t1 | AT5G11490 | 25.846 | 325 | 206 | 13 | 347 | 661 | 220 | 519 | 2.09e-13 | 75.5 |
Find 253 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTCTGAACAGTCAATTTC+TGG | 0.160270 | 5.3:-64595787 | MS.gene29487:CDS |
| CTGAAAGCTTCAATATTAAT+TGG | 0.185952 | 5.3:+64594833 | None:intergenic |
| TATTAAGGTTGCTCCTAAAT+TGG | 0.194908 | 5.3:-64601130 | MS.gene29487:CDS |
| ACGATCAAGTTCTACTATTA+AGG | 0.238402 | 5.3:-64601145 | MS.gene29487:CDS |
| TTGGCTTAAATTTCTTAAAT+AGG | 0.238571 | 5.3:-64593876 | MS.gene29487:CDS |
| TGGGGTCAAATAATGCTAAT+TGG | 0.246734 | 5.3:-64601378 | MS.gene29487:CDS |
| GCTTGAAGTTTCAGATAAAA+AGG | 0.248262 | 5.3:+64595423 | None:intergenic |
| GAAACTTCTCTCGAGCAATT+TGG | 0.257524 | 5.3:-64596121 | MS.gene29487:CDS |
| TTCCTAAGCTCCACGATTTA+CGG | 0.258340 | 5.3:-64601819 | MS.gene29487:CDS |
| TATAGCGATTCATCCAATTT+AGG | 0.268455 | 5.3:+64601117 | None:intergenic |
| ATTATTTGAAAATTGTTCTT+TGG | 0.273575 | 5.3:-64595371 | MS.gene29487:CDS |
| GTGGATGATCCTGGTACATC+TGG | 0.274029 | 5.3:-64595841 | MS.gene29487:CDS |
| TATGGAAGGCTACAAGGTTT+TGG | 0.275111 | 5.3:+64595907 | None:intergenic |
| ATTCATTCTTAAAGAATTTC+AGG | 0.279242 | 5.3:-64599216 | MS.gene29487:intron |
| GGGGATCACTCTGGCACTTC+TGG | 0.282683 | 5.3:-64595649 | MS.gene29487:CDS |
| GAAGGGTACCTTCTCATAAC+TGG | 0.285739 | 5.3:+64598283 | None:intergenic |
| TTAGGTCTCACTGTGCATTA+AGG | 0.286171 | 5.3:-64596244 | MS.gene29487:intron |
| AATTCAGTTGGTCCGTAGTT+TGG | 0.302335 | 5.3:-64598016 | MS.gene29487:CDS |
| ATTAAACCATTTCCATTTGC+AGG | 0.308293 | 5.3:+64595457 | None:intergenic |
| TTTGAGAGCTTCACATTTCT+CGG | 0.320089 | 5.3:+64603319 | None:intergenic |
| ATCGACAGCAATGCTTCATT+TGG | 0.328968 | 5.3:+64602012 | None:intergenic |
| CATGGAGGCAATCTCTTCTC+TGG | 0.330338 | 5.3:-64594996 | MS.gene29487:CDS |
| TGCAAATGGAAATGGTTTAA+TGG | 0.333946 | 5.3:-64595455 | MS.gene29487:CDS |
| GCAATGATTGTCTGGTTGTT+GGG | 0.337044 | 5.3:-64597967 | MS.gene29487:CDS |
| CAAAATGTGGAAGCGGAAAA+TGG | 0.345825 | 5.3:-64596093 | MS.gene29487:CDS |
| CTTCATTTGGTGCAGATGAT+TGG | 0.348331 | 5.3:-64601537 | MS.gene29487:intron |
| CAAACTTATGAGTGGGTAAC+AGG | 0.352772 | 5.3:+64593829 | None:intergenic |
| GAAAAGGAATCTCACCTTCC+AGG | 0.359392 | 5.3:-64594809 | MS.gene29487:CDS |
| AAACGTTGCGTCGCAGTCAT+TGG | 0.371905 | 5.3:-64603108 | MS.gene29487:CDS |
| TCAGAGCCAACAGTCCTTCC+AGG | 0.377842 | 5.3:+64598492 | None:intergenic |
| AGAAGAGGGTGTACTAATTC+AGG | 0.379871 | 5.3:-64598345 | MS.gene29487:CDS |
| TATACTTTCTATTATTGCTT+CGG | 0.380033 | 5.3:-64599252 | MS.gene29487:CDS |
| TTGGATCCTGCAAATGGAAA+TGG | 0.380142 | 5.3:-64595463 | MS.gene29487:CDS |
| TTAATTACAGTTACTGTTGA+AGG | 0.380398 | 5.3:-64593955 | MS.gene29487:CDS |
| TGCAATGATTGTCTGGTTGT+TGG | 0.381752 | 5.3:-64597968 | MS.gene29487:CDS |
| GGCAGCAACGAGACTGTATC+TGG | 0.382076 | 5.3:-64595748 | MS.gene29487:CDS |
| AGTAACCTGAGGGAAGAAAT+TGG | 0.383405 | 5.3:+64603259 | None:intergenic |
| GCCGGCACAGTTGCTGCCAT+TGG | 0.389628 | 5.3:-64598554 | MS.gene29487:CDS |
| AGAATAACCATTGGAGATAT+CGG | 0.408234 | 5.3:-64595517 | MS.gene29487:CDS |
| ACTAACTGTGAAAGAGATCC+AGG | 0.410053 | 5.3:+64595961 | None:intergenic |
| TCTTCGTGTAATAAAGATCA+TGG | 0.410319 | 5.3:-64601297 | MS.gene29487:CDS |
| TACAGATACAAGAGAAAGAT+TGG | 0.410808 | 5.3:+64594065 | None:intergenic |
| GGAGCGATGTTAACGTCTTC+GGG | 0.413968 | 5.3:+64603365 | None:intergenic |
| CATCTGAACCTATCAATGAT+AGG | 0.415212 | 5.3:-64595993 | MS.gene29487:CDS |
| TAGGTCTCACTGTGCATTAA+GGG | 0.417194 | 5.3:-64596243 | MS.gene29487:CDS |
| GGGGTCAAATAATGCTAATT+GGG | 0.421274 | 5.3:-64601377 | MS.gene29487:CDS |
| TTCACTTCCTTTAGATGACT+TGG | 0.421488 | 5.3:+64595573 | None:intergenic |
| AGGAGCGATGTTAACGTCTT+CGG | 0.421496 | 5.3:+64603364 | None:intergenic |
| GTCCGTTTCGCTTTCTGACC+AGG | 0.422477 | 5.3:+64594971 | None:intergenic |
| CTGCGCGTGCAATGATTGTC+TGG | 0.423590 | 5.3:-64597975 | MS.gene29487:CDS |
| TATTGAAGCTTTCAGAGAAA+AGG | 0.423988 | 5.3:-64594825 | MS.gene29487:CDS |
| ATTCCGCCGTCGCTCCGAAT+TGG | 0.427634 | 5.3:+64603450 | None:intergenic |
| TAACTTTATAATAGATATGA+TGG | 0.428377 | 5.3:+64598313 | None:intergenic |
| TTTATCAATATGAGATTTCA+AGG | 0.431183 | 5.3:+64595033 | None:intergenic |
| TGCAGGTGTACACTGGATAA+TGG | 0.440796 | 5.3:-64601004 | MS.gene29487:CDS |
| AACTGCGAAGAGACTGTGTT+TGG | 0.442036 | 5.3:-64593895 | MS.gene29487:CDS |
| ACCCAAGTGCTACACTCTCT+TGG | 0.442514 | 5.3:-64595482 | MS.gene29487:CDS |
| GTTTCTGAAATCTGAATCAA+TGG | 0.444058 | 5.3:+64595700 | None:intergenic |
| TGTTTCCAATTTCTTCCCTC+AGG | 0.444119 | 5.3:-64603264 | MS.gene29487:intron |
| ATGTCAGGCCTTAGCTTGAC+AGG | 0.445975 | 5.3:+64594878 | None:intergenic |
| GGGACACTAACCCATTGGTT+AGG | 0.450223 | 5.3:-64601966 | MS.gene29487:CDS |
| ACCTCTTGTCCTTGTTGCTG+TGG | 0.451472 | 5.3:-64601896 | MS.gene29487:CDS |
| TTTGGGAGAGGCTCATAGCC+TGG | 0.452053 | 5.3:+64595925 | None:intergenic |
| TCAAATAAAAGCGCTTAAGC+TGG | 0.452336 | 5.3:-64599276 | MS.gene29487:CDS |
| ACAATCATTGCACGCGCAGC+AGG | 0.452481 | 5.3:+64597979 | None:intergenic |
| CCGGCAGCAAATCTCCTGTC+TGG | 0.454570 | 5.3:+64598572 | None:intergenic |
| ATCTTCCAGTGTTACATTGA+AGG | 0.455279 | 5.3:-64601186 | MS.gene29487:CDS |
| GCTAAAACGCAAACACAAAC+CGG | 0.457720 | 5.3:+64594008 | None:intergenic |
| GATCACGAATATCATAGTTC+AGG | 0.461133 | 5.3:+64596160 | None:intergenic |
| TTAGTATTTCATGCAGCTCC+AGG | 0.461403 | 5.3:-64595943 | MS.gene29487:CDS |
| TTTACAAAGTATCCAATGTC+AGG | 0.462160 | 5.3:+64594863 | None:intergenic |
| AAGGACTGTTGGCTCTGATT+AGG | 0.462793 | 5.3:-64598487 | MS.gene29487:CDS |
| AGATAAAACCTATCATTGAT+AGG | 0.464643 | 5.3:+64595985 | None:intergenic |
| GTATTGTTCTTTGGGTGAGA+TGG | 0.466808 | 5.3:-64597941 | MS.gene29487:CDS |
| GGACACTAACCCATTGGTTA+GGG | 0.467416 | 5.3:-64601965 | MS.gene29487:CDS |
| CAGAGAAGAGATTGCCTCCA+TGG | 0.468576 | 5.3:+64594997 | None:intergenic |
| GGCGGATTCCCGCCATGGCT+CGG | 0.469183 | 5.3:+64601932 | None:intergenic |
| CCGCCGTCGCTCCGAATTGG+TGG | 0.471478 | 5.3:+64603453 | None:intergenic |
| TGACCCCACTCTTCCACATC+GGG | 0.473623 | 5.3:+64601393 | None:intergenic |
| TTGGACCTATGTAATTGAAC+TGG | 0.473757 | 5.3:-64596193 | MS.gene29487:CDS |
| TTGCTGAAGATCTTCGAACT+TGG | 0.474557 | 5.3:+64595539 | None:intergenic |
| GTTGCTGCCACTGACTTCAC+TGG | 0.474867 | 5.3:+64595762 | None:intergenic |
| CGGAATCGAATCTGCTATCA+AGG | 0.475985 | 5.3:+64603339 | None:intergenic |
| ATTCTGCAAGCACGCTAGAT+TGG | 0.481320 | 5.3:+64596052 | None:intergenic |
| ACTCTCTTGGATCCTGCAAA+TGG | 0.483926 | 5.3:-64595469 | MS.gene29487:CDS |
| GGGCGGCGTCAGTGCCGATT+CGG | 0.484741 | 5.3:+64603399 | None:intergenic |
| TGGGCACTCCGAGCCATGGC+GGG | 0.486636 | 5.3:-64601940 | MS.gene29487:CDS |
| TTGTTAAACGATCATTCCCC+CGG | 0.489484 | 5.3:-64601510 | MS.gene29487:CDS |
| ATCCGTAAATCGTGGAGCTT+AGG | 0.489545 | 5.3:+64601817 | None:intergenic |
| GCAATCTCTTCTCTGGAGCC+TGG | 0.490498 | 5.3:-64594989 | MS.gene29487:CDS |
| ACCTTCACAGAAGCAATCAA+TGG | 0.490864 | 5.3:+64593919 | None:intergenic |
| TCTAACCTCGATGATGCATC+CGG | 0.492003 | 5.3:-64594027 | MS.gene29487:CDS |
| CCTCTTGTCCTTGTTGCTGT+GGG | 0.492340 | 5.3:-64601895 | MS.gene29487:CDS |
| GCATTAAGGGAGAAGATAGT+TGG | 0.492928 | 5.3:-64596230 | MS.gene29487:CDS |
| TGCATGCCTGGAAGGACTGT+TGG | 0.494258 | 5.3:-64598498 | MS.gene29487:CDS |
| GCAGCTGCTCCAACAACCCC+GGG | 0.496265 | 5.3:+64601492 | None:intergenic |
| AGCAGCTGCTCCAACAACCC+CGG | 0.500132 | 5.3:+64601491 | None:intergenic |
| TCAGAGGAGGTGGATGATCC+TGG | 0.504366 | 5.3:-64595850 | MS.gene29487:CDS |
| GTGCTTGCAGAATGCATCTT+CGG | 0.506831 | 5.3:-64596042 | MS.gene29487:CDS |
| GCTTCAATATTAATTGGAAG+TGG | 0.508821 | 5.3:+64594839 | None:intergenic |
| TTAATGAAAATCAGAATGGC+GGG | 0.510610 | 5.3:-64595669 | MS.gene29487:CDS |
| CAACGAAGTCTCTCGAGTCT+TGG | 0.511409 | 5.3:-64595609 | MS.gene29487:CDS |
| TTCTATCCAAACTTATGAGT+GGG | 0.511712 | 5.3:+64593822 | None:intergenic |
| GAGACTGTATCTGGCGATGA+AGG | 0.513926 | 5.3:-64595739 | MS.gene29487:CDS |
| TTAGAGGCAAAAGGTGGTGA+TGG | 0.514821 | 5.3:+64594933 | None:intergenic |
| TCAACGGCGGTGTTCCGAAT+CGG | 0.514995 | 5.3:-64603413 | MS.gene29487:CDS |
| ACAGTAACTGTAATTAAGCA+TGG | 0.515018 | 5.3:+64593961 | None:intergenic |
| GCTCAGCATCTTCAATGCTA+AGG | 0.515822 | 5.3:+64594092 | None:intergenic |
| AGTGCCCATGCCCTAACCAA+TGG | 0.516304 | 5.3:+64601955 | None:intergenic |
| CCATACGTTGATCAGTATGA+TGG | 0.516999 | 5.3:-64595889 | MS.gene29487:CDS |
| GAACCATCCAAGTCATCTAA+AGG | 0.517839 | 5.3:-64595580 | MS.gene29487:CDS |
| GTACACCCTCTTCTCCATCC+AGG | 0.520755 | 5.3:+64598354 | None:intergenic |
| GATCGTGATAAGTACTCGTC+TGG | 0.522233 | 5.3:+64601162 | None:intergenic |
| ATGGCAACTGCATGCCTGGA+AGG | 0.522537 | 5.3:-64598506 | MS.gene29487:CDS |
| TCATCGAGGTTAGAAGCTAC+TGG | 0.523704 | 5.3:+64594036 | None:intergenic |
| CTTCCTTTAGATGACTTGGA+TGG | 0.523928 | 5.3:+64595577 | None:intergenic |
| AGTGTACATCACCATTGTTA+TGG | 0.524053 | 5.3:-64601056 | MS.gene29487:CDS |
| GTGCCCATGCCCTAACCAAT+GGG | 0.524137 | 5.3:+64601956 | None:intergenic |
| TAAACCAGCGCTAGTTTCCA+TGG | 0.526393 | 5.3:-64595014 | MS.gene29487:CDS |
| TCTTCCAGTGTTACATTGAA+GGG | 0.526686 | 5.3:-64601185 | MS.gene29487:CDS |
| AGTACTGTACTCAACATCCT+TGG | 0.527822 | 5.3:+64597916 | None:intergenic |
| CAATGATTGTCTGGTTGTTG+GGG | 0.529613 | 5.3:-64597966 | MS.gene29487:CDS |
| CAATTGTTTCCAGAAGGACC+TGG | 0.533612 | 5.3:-64601989 | MS.gene29487:CDS |
| GGCATGGGCACTCCGAGCCA+TGG | 0.535022 | 5.3:-64601944 | MS.gene29487:CDS |
| TCTTCCCGATGTGGAAGAGT+GGG | 0.535399 | 5.3:-64601397 | MS.gene29487:CDS |
| CAGAATGGCGGGGATCACTC+TGG | 0.537173 | 5.3:-64595658 | MS.gene29487:CDS |
| GCCTCCATGGAAACTAGCGC+TGG | 0.537409 | 5.3:+64595010 | None:intergenic |
| ATTATATTCGAGATCCAGAC+AGG | 0.538111 | 5.3:-64598586 | MS.gene29487:CDS |
| ATACTTCTAAACATACAAGA+TGG | 0.538534 | 5.3:+64595392 | None:intergenic |
| CCCATTGATTGCTTCTGTGA+AGG | 0.540095 | 5.3:-64593920 | MS.gene29487:CDS |
| ATTACACGAAGAAAACATGA+TGG | 0.540141 | 5.3:+64601308 | None:intergenic |
| CTCACATATTCAAACATCCC+TGG | 0.540424 | 5.3:+64594791 | None:intergenic |
| ATGAAGGCGGATTCCCGCCA+TGG | 0.540748 | 5.3:+64601927 | None:intergenic |
| GAAGTCTCTCGAGTCTTGGT+TGG | 0.541737 | 5.3:-64595605 | MS.gene29487:CDS |
| CTTTGGGTGAGATGGTTCCA+AGG | 0.541790 | 5.3:-64597933 | MS.gene29487:CDS |
| TAACAGTGATTCAGAGGAGG+TGG | 0.542007 | 5.3:-64595860 | MS.gene29487:CDS |
| ACCAATGGCAGCAACTGTGC+CGG | 0.542026 | 5.3:+64598553 | None:intergenic |
| TGGATGATCCTGGTACATCT+GGG | 0.542973 | 5.3:-64595840 | MS.gene29487:CDS |
| ACACTGGATAATGGCGCCTA+AGG | 0.543775 | 5.3:-64600995 | MS.gene29487:CDS |
| TTATAAGGTTATAATTCAGT+TGG | 0.544384 | 5.3:-64598028 | MS.gene29487:intron |
| GCATAGCTTTAGCAAACACT+TGG | 0.544634 | 5.3:+64599354 | None:intergenic |
| AACTGTAATTAAGCATGGGA+TGG | 0.544904 | 5.3:+64593966 | None:intergenic |
| TAACCCATTGGTTAGGGCAT+GGG | 0.547486 | 5.3:-64601959 | MS.gene29487:CDS |
| GTCATTGGAAGTGAAGAAGC+TGG | 0.548244 | 5.3:-64603093 | MS.gene29487:CDS |
| CAGATCCCTGGATGGAGAAG+AGG | 0.548321 | 5.3:-64598360 | MS.gene29487:CDS |
| GCAGTTGTACTTGCAGCTGC+AGG | 0.551272 | 5.3:-64601021 | MS.gene29487:CDS |
| CGGTATGTGATAGCGAGGCA+TGG | 0.551882 | 5.3:-64601345 | MS.gene29487:CDS |
| ATCACTGAGCAAAGCTTCAA+CGG | 0.552688 | 5.3:-64603429 | MS.gene29487:CDS |
| AGAAATGTGAAGCTCTCAAA+CGG | 0.553733 | 5.3:-64603316 | MS.gene29487:CDS |
| CCGTAGTTTGGATACAATCA+AGG | 0.553966 | 5.3:-64598004 | MS.gene29487:CDS |
| CTTACCACGTATCTTGAAGC+AGG | 0.555381 | 5.3:+64600922 | None:intergenic |
| AATTGTTTCCAGAAGGACCT+GGG | 0.557416 | 5.3:-64601988 | MS.gene29487:CDS |
| ATGAAGAAGTCTTCATAATG+TGG | 0.558926 | 5.3:+64599317 | None:intergenic |
| ATAAATTCCCTGTCAAGCTA+AGG | 0.559978 | 5.3:-64594886 | MS.gene29487:CDS |
| CCTTCACAGAAGCAATCAAT+GGG | 0.560302 | 5.3:+64593920 | None:intergenic |
| GGTATGTGATAGCGAGGCAT+GGG | 0.560492 | 5.3:-64601344 | MS.gene29487:CDS |
| CAGTACTCAAGTATCTTGCC+TGG | 0.560507 | 5.3:-64597900 | MS.gene29487:CDS |
| TCAACGTATGGAAGGCTACA+AGG | 0.562471 | 5.3:+64595901 | None:intergenic |
| GTGCGGTGAAATCAGATCCC+TGG | 0.563526 | 5.3:-64598372 | MS.gene29487:CDS |
| GGAGTCTCAAAATGTGGAAG+CGG | 0.563531 | 5.3:-64596100 | MS.gene29487:CDS |
| CGATATAGTCTTACTAGATG+AGG | 0.567817 | 5.3:-64595338 | MS.gene29487:CDS |
| AAAAGGAATCTCACCTTCCA+GGG | 0.568458 | 5.3:-64594808 | MS.gene29487:CDS |
| TCTTCAGCAAGAATAACCAT+TGG | 0.568661 | 5.3:-64595526 | MS.gene29487:CDS |
| CGTCTTCGGGATCGTCGTAG+AGG | 0.570073 | 5.3:+64603378 | None:intergenic |
| TTGCAGCTGCAGGTGTACAC+TGG | 0.570380 | 5.3:-64601011 | MS.gene29487:CDS |
| CCATCATACTGATCAACGTA+TGG | 0.570410 | 5.3:+64595889 | None:intergenic |
| TTTCTATCCAAACTTATGAG+TGG | 0.570596 | 5.3:+64593821 | None:intergenic |
| GTCGATCAATTGTTTCCAGA+AGG | 0.573460 | 5.3:-64601995 | MS.gene29487:CDS |
| TTACTCGCTCTCATCGCTCA+AGG | 0.574127 | 5.3:-64603293 | MS.gene29487:CDS |
| ACATGCATCTGCAATTGAAG+AGG | 0.574911 | 5.3:-64601788 | MS.gene29487:intron |
| TCAGAAGACCCAGATGTACC+AGG | 0.574948 | 5.3:+64595832 | None:intergenic |
| TTGAAGCAGGCGATGATCGA+AGG | 0.575259 | 5.3:+64600935 | None:intergenic |
| AATGTTAATGAAAATCAGAA+TGG | 0.576541 | 5.3:-64595673 | MS.gene29487:CDS |
| TACACCCTCTTCTCCATCCA+GGG | 0.578463 | 5.3:+64598355 | None:intergenic |
| AAATGGTGAATCACAGAGGA+AGG | 0.579441 | 5.3:-64596076 | MS.gene29487:CDS |
| CTATCATTGATAGGTTCAGA+TGG | 0.579787 | 5.3:+64595994 | None:intergenic |
| AGCCTGGTCAGAAAGCGAAA+CGG | 0.581259 | 5.3:-64594973 | MS.gene29487:CDS |
| TTCTTCCCGATGTGGAAGAG+TGG | 0.581596 | 5.3:-64601398 | MS.gene29487:CDS |
| ATCCAAGAGAGTGTAGCACT+TGG | 0.583531 | 5.3:+64595480 | None:intergenic |
| GTCAAGCTAAGGCCTGACAT+TGG | 0.584364 | 5.3:-64594875 | MS.gene29487:CDS |
| AGATCCCTGGATGGAGAAGA+GGG | 0.584446 | 5.3:-64598359 | MS.gene29487:CDS |
| CAAGAGGTGCAATTGCATGA+AGG | 0.584601 | 5.3:+64601911 | None:intergenic |
| TCCAAGAGAGTGTAGCACTT+GGG | 0.585946 | 5.3:+64595481 | None:intergenic |
| CACAGAAGCAATCAATGGGT+CGG | 0.592933 | 5.3:+64593924 | None:intergenic |
| TACTTCTAAACATACAAGAT+GGG | 0.594433 | 5.3:+64595393 | None:intergenic |
| CCTTGATTGTATCCAAACTA+CGG | 0.594869 | 5.3:+64598004 | None:intergenic |
| GTTAATGAAAATCAGAATGG+CGG | 0.595954 | 5.3:-64595670 | MS.gene29487:CDS |
| ATGGGCACTCCGAGCCATGG+CGG | 0.596177 | 5.3:-64601941 | MS.gene29487:CDS |
| GTCTTCGGGATCGTCGTAGA+GGG | 0.596516 | 5.3:+64603379 | None:intergenic |
| CAATTTGGAGTCTCAAAATG+TGG | 0.598371 | 5.3:-64596106 | MS.gene29487:CDS |
| ATGGTGATGTACACTGCAGT+AGG | 0.599426 | 5.3:+64601064 | None:intergenic |
| CAGGGATGTTTGAATATGTG+AGG | 0.600419 | 5.3:-64594790 | MS.gene29487:CDS |
| CTGGATAATGGCGCCTAAGG+AGG | 0.601876 | 5.3:-64600992 | MS.gene29487:CDS |
| CAGTAACTGTAATTAAGCAT+GGG | 0.602872 | 5.3:+64593962 | None:intergenic |
| CTGGCCCTTCAATGTAACAC+TGG | 0.604405 | 5.3:+64601181 | None:intergenic |
| CCCACAGCAACAAGGACAAG+AGG | 0.606377 | 5.3:+64601895 | None:intergenic |
| TAAGCTCCACGATTTACGGA+TGG | 0.608419 | 5.3:-64601815 | MS.gene29487:CDS |
| TTCGGGATCGTCGTAGAGGG+CGG | 0.609997 | 5.3:+64603382 | None:intergenic |
| CATACTGATCAACGTATGGA+AGG | 0.611375 | 5.3:+64595893 | None:intergenic |
| TTGACCCCACTCTTCCACAT+CGG | 0.614765 | 5.3:+64601392 | None:intergenic |
| CAATCATTGCACGCGCAGCA+GGG | 0.614853 | 5.3:+64597980 | None:intergenic |
| CCAGACAGGAGATTTGCTGC+CGG | 0.616334 | 5.3:-64598572 | MS.gene29487:CDS |
| CTAACCCATTGGTTAGGGCA+TGG | 0.619044 | 5.3:-64601960 | MS.gene29487:CDS |
| AGCTGTTAACAGTGATTCAG+AGG | 0.621517 | 5.3:-64595866 | MS.gene29487:CDS |
| CTTGCAGAATGCATCTTCGG+TGG | 0.622297 | 5.3:-64596039 | MS.gene29487:CDS |
| CTGTTGGCTCTGATTAGGCA+AGG | 0.622813 | 5.3:-64598482 | MS.gene29487:intron |
| GTTCCACCAATTCGGAGCGA+CGG | 0.624950 | 5.3:-64603456 | MS.gene29487:CDS |
| AAGGGTACCTTCTCATAACT+GGG | 0.625752 | 5.3:+64598284 | None:intergenic |
| TCCTTCTGAAGTAAAACACC+AGG | 0.627310 | 5.3:+64597882 | None:intergenic |
| GGAAAGCAACTCTTTAACAG+AGG | 0.628805 | 5.3:-64594139 | MS.gene29487:CDS |
| CATATTCAAACATCCCTGGA+AGG | 0.630328 | 5.3:+64594795 | None:intergenic |
| TGTAGCACTTGGGTTTAACA+CGG | 0.630885 | 5.3:+64595491 | None:intergenic |
| ACGGCTTCCGATATCTCCAA+TGG | 0.640314 | 5.3:+64595510 | None:intergenic |
| TAATGAAAATCAGAATGGCG+GGG | 0.640346 | 5.3:-64595668 | MS.gene29487:CDS |
| TAATGTGGAGCAAATAGAGA+GGG | 0.641141 | 5.3:+64599332 | None:intergenic |
| CTCAGCCAGTTCAATTACAT+AGG | 0.646264 | 5.3:+64596188 | None:intergenic |
| GTTCATCCATCCGTAAATCG+TGG | 0.648829 | 5.3:+64601809 | None:intergenic |
| TTCTAAACATACAAGATGGG+AGG | 0.651340 | 5.3:+64595396 | None:intergenic |
| AATGATTGTCTGGTTGTTGG+GGG | 0.651414 | 5.3:-64597965 | MS.gene29487:CDS |
| GGCGATGAAGGTAACAACAA+TGG | 0.651855 | 5.3:-64595727 | MS.gene29487:CDS |
| CCACCAATTCGGAGCGACGG+CGG | 0.654914 | 5.3:-64603453 | MS.gene29487:CDS |
| AGAACCACCCAGTTATGAGA+AGG | 0.655244 | 5.3:-64598291 | MS.gene29487:intron |
| GATTCTTAATACAATCACAA+AGG | 0.662421 | 5.3:-64597842 | MS.gene29487:intron |
| CTTGCAGCAATGAAGTTGTG+AGG | 0.663822 | 5.3:-64601089 | MS.gene29487:CDS |
| TCACATTCTAAAGCTGAACA+AGG | 0.664089 | 5.3:-64594160 | MS.gene29487:CDS |
| AGTAAGACTATATCGAACAT+CGG | 0.664529 | 5.3:+64595346 | None:intergenic |
| ATTGTTTCCAGAAGGACCTG+GGG | 0.665392 | 5.3:-64601987 | MS.gene29487:CDS |
| TCGAATCTGCTATCAAGGAG+AGG | 0.669151 | 5.3:+64603344 | None:intergenic |
| TCTGGTTCCAGTGAAGTCAG+TGG | 0.670241 | 5.3:-64595769 | MS.gene29487:CDS |
| TCTCTGGGCACATAAACCAA+TGG | 0.670656 | 5.3:+64598538 | None:intergenic |
| GTGTGAGATTCTTCCCGATG+TGG | 0.670784 | 5.3:-64601406 | MS.gene29487:CDS |
| CTAACTGTGAAAGAGATCCA+GGG | 0.670939 | 5.3:+64595962 | None:intergenic |
| CTTCCCGATGTGGAAGAGTG+GGG | 0.673122 | 5.3:-64601396 | MS.gene29487:CDS |
| GAGGTGCAATTGCATGAAGG+CGG | 0.677361 | 5.3:+64601914 | None:intergenic |
| GGATGTTTGAATATGTGAGG+AGG | 0.679287 | 5.3:-64594787 | MS.gene29487:intron |
| ATAATGTGGAGCAAATAGAG+AGG | 0.682055 | 5.3:+64599331 | None:intergenic |
| TGTCAGGCCTTAGCTTGACA+GGG | 0.683890 | 5.3:+64594879 | None:intergenic |
| TACCACTGAGAAGACTGTGT+CGG | 0.685950 | 5.3:-64601223 | MS.gene29487:CDS |
| TGTTAACAGTGATTCAGAGG+AGG | 0.690003 | 5.3:-64595863 | MS.gene29487:CDS |
| ACTTATGAGTGGGTAACAGG+TGG | 0.692464 | 5.3:+64593832 | None:intergenic |
| ACTGAGCAAAGCTTCAACGG+CGG | 0.692518 | 5.3:-64603426 | MS.gene29487:CDS |
| ATCGCCTGCTTCAAGATACG+TGG | 0.696189 | 5.3:-64600926 | MS.gene29487:intron |
| GGTGAAATCAGATCCCTGGA+TGG | 0.698792 | 5.3:-64598368 | MS.gene29487:CDS |
| AGCTGCTCCAACAACCCCGG+GGG | 0.704262 | 5.3:+64601494 | None:intergenic |
| TTCCGACACAGTCTTCTCAG+TGG | 0.704709 | 5.3:+64601221 | None:intergenic |
| TTGTTTCCAGAAGGACCTGG+GGG | 0.705561 | 5.3:-64601986 | MS.gene29487:CDS |
| CAGCTGCTCCAACAACCCCG+GGG | 0.718459 | 5.3:+64601493 | None:intergenic |
| CTGTTATGAGACCATAACAA+TGG | 0.726798 | 5.3:+64601045 | None:intergenic |
| CGGAAAATGGTGAATCACAG+AGG | 0.732480 | 5.3:-64596080 | MS.gene29487:CDS |
| ACCAGCGCTAGTTTCCATGG+AGG | 0.738902 | 5.3:-64595011 | MS.gene29487:CDS |
| GGTACCTTCTCATAACTGGG+TGG | 0.739084 | 5.3:+64598287 | None:intergenic |
| GATCGAAGGACAAACAACAG+TGG | 0.758896 | 5.3:+64600949 | None:intergenic |
| ACAAACCGGATGCATCATCG+AGG | 0.763115 | 5.3:+64594022 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 64593834 | 64603480 | 64593834 | ID=MS.gene29487 |
| chr5.3 | mRNA | 64593834 | 64603480 | 64593834 | ID=MS.gene29487.t1;Parent=MS.gene29487 |
| chr5.3 | exon | 64603265 | 64603480 | 64603265 | ID=MS.gene29487.t1.exon1;Parent=MS.gene29487.t1 |
| chr5.3 | CDS | 64603265 | 64603480 | 64603265 | ID=cds.MS.gene29487.t1;Parent=MS.gene29487.t1 |
| chr5.3 | exon | 64603062 | 64603138 | 64603062 | ID=MS.gene29487.t1.exon2;Parent=MS.gene29487.t1 |
| chr5.3 | CDS | 64603062 | 64603138 | 64603062 | ID=cds.MS.gene29487.t1;Parent=MS.gene29487.t1 |
| chr5.3 | exon | 64601789 | 64602038 | 64601789 | ID=MS.gene29487.t1.exon3;Parent=MS.gene29487.t1 |
| chr5.3 | CDS | 64601789 | 64602038 | 64601789 | ID=cds.MS.gene29487.t1;Parent=MS.gene29487.t1 |
| chr5.3 | exon | 64600927 | 64601544 | 64600927 | ID=MS.gene29487.t1.exon4;Parent=MS.gene29487.t1 |
| chr5.3 | CDS | 64600927 | 64601544 | 64600927 | ID=cds.MS.gene29487.t1;Parent=MS.gene29487.t1 |
| chr5.3 | exon | 64599217 | 64599390 | 64599217 | ID=MS.gene29487.t1.exon5;Parent=MS.gene29487.t1 |
| chr5.3 | CDS | 64599217 | 64599390 | 64599217 | ID=cds.MS.gene29487.t1;Parent=MS.gene29487.t1 |
| chr5.3 | exon | 64598483 | 64598609 | 64598483 | ID=MS.gene29487.t1.exon6;Parent=MS.gene29487.t1 |
| chr5.3 | CDS | 64598483 | 64598609 | 64598483 | ID=cds.MS.gene29487.t1;Parent=MS.gene29487.t1 |
| chr5.3 | exon | 64598292 | 64598401 | 64598292 | ID=MS.gene29487.t1.exon7;Parent=MS.gene29487.t1 |
| chr5.3 | CDS | 64598292 | 64598401 | 64598292 | ID=cds.MS.gene29487.t1;Parent=MS.gene29487.t1 |
| chr5.3 | exon | 64597843 | 64598043 | 64597843 | ID=MS.gene29487.t1.exon8;Parent=MS.gene29487.t1 |
| chr5.3 | CDS | 64597843 | 64598043 | 64597843 | ID=cds.MS.gene29487.t1;Parent=MS.gene29487.t1 |
| chr5.3 | exon | 64595286 | 64596262 | 64595286 | ID=MS.gene29487.t1.exon9;Parent=MS.gene29487.t1 |
| chr5.3 | CDS | 64595286 | 64596262 | 64595286 | ID=cds.MS.gene29487.t1;Parent=MS.gene29487.t1 |
| chr5.3 | exon | 64594788 | 64595057 | 64594788 | ID=MS.gene29487.t1.exon10;Parent=MS.gene29487.t1 |
| chr5.3 | CDS | 64594788 | 64595057 | 64594788 | ID=cds.MS.gene29487.t1;Parent=MS.gene29487.t1 |
| chr5.3 | exon | 64593834 | 64594197 | 64593834 | ID=MS.gene29487.t1.exon11;Parent=MS.gene29487.t1 |
| chr5.3 | CDS | 64593834 | 64594197 | 64593834 | ID=cds.MS.gene29487.t1;Parent=MS.gene29487.t1 |
| Gene Sequence |
| Protein sequence |
