Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058438 | MS.gene29652 | 0.986199 | 5.36E-166 | -1.69E-46 |
| MS.gene060910 | MS.gene29652 | 0.937462 | 3.33E-98 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAGGTGATTCTTATGTTT+TGG | 0.220436 | 5.1:+64861227 | MS.gene29652:CDS |
| GTTACTTCTCTCATCTTATA+AGG | 0.247292 | 5.1:+64861090 | MS.gene29652:CDS |
| ACTGTTGGTGCTGGTGTTGC+TGG | 0.278594 | 5.1:+64861464 | MS.gene29652:CDS |
| CGATGTAGGCGAAGTTAATT+CGG | 0.329255 | 5.1:+64861412 | MS.gene29652:intron |
| TATTGCTCTGGTTGTACTCT+TGG | 0.344765 | 5.1:+64861487 | MS.gene29652:CDS |
| GGGTTCAATTTCATTGATGA+TGG | 0.393070 | 5.1:-64860911 | None:intergenic |
| CTCATCAACATATCGGCATC+AGG | 0.407565 | 5.1:+64861929 | MS.gene29652:CDS |
| ATTGCTCTGGTTGTACTCTT+GGG | 0.449876 | 5.1:+64861488 | MS.gene29652:CDS |
| TGGTGTTGCTGGTATTGCTC+TGG | 0.454038 | 5.1:+64861475 | MS.gene29652:CDS |
| AATGTTATCACTGTTGGTGC+TGG | 0.466483 | 5.1:+64861455 | MS.gene29652:CDS |
| CATTTCCCTCATCAACATAT+CGG | 0.470848 | 5.1:+64861922 | MS.gene29652:CDS |
| TCAAGAGCAAGAACTGGAAC+AGG | 0.482132 | 5.1:-64861053 | None:intergenic |
| CCAGTTCTTGCTCTTGACAT+CGG | 0.484096 | 5.1:+64861059 | MS.gene29652:CDS |
| GCAGTTATCAACATCTTCGT+TGG | 0.491361 | 5.1:+64861885 | MS.gene29652:intron |
| CTCTGGTTGTACTCTTGGGA+AGG | 0.498339 | 5.1:+64861492 | MS.gene29652:CDS |
| CCGATGTCAAGAGCAAGAAC+TGG | 0.503027 | 5.1:-64861059 | None:intergenic |
| TGAACAAACATGATGAAAAG+AGG | 0.506916 | 5.1:-64861119 | None:intergenic |
| GGTTCAATTTCATTGATGAT+GGG | 0.521972 | 5.1:-64860910 | None:intergenic |
| ATATCGGCATCAGGGCAACT+TGG | 0.528755 | 5.1:+64861938 | MS.gene29652:CDS |
| ATATTTAGGGGATAGAATGA+AGG | 0.532536 | 5.1:+64861210 | MS.gene29652:intron |
| GATGCTAATGTTATCACTGT+TGG | 0.589755 | 5.1:+64861449 | MS.gene29652:CDS |
| TCATCAACATATCGGCATCA+GGG | 0.604907 | 5.1:+64861930 | MS.gene29652:CDS |
| AACTGGAACAGGAACAACAA+AGG | 0.628523 | 5.1:-64861042 | None:intergenic |
| CTGATGCCGATATGTTGATG+AGG | 0.666580 | 5.1:-64861928 | None:intergenic |
| TGATGCCGATATGTTGATGA+GGG | 0.679239 | 5.1:-64861927 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATAGATTGATTTTATATTT+GGG | + | chr5.1:64861848-64861867 | MS.gene29652:intron | 10.0% |
| !! | TACTGGATTTATTATATTTA+GGG | + | chr5.1:64861197-64861216 | MS.gene29652:intron | 15.0% |
| !! | TTACTGGATTTATTATATTT+AGG | + | chr5.1:64861196-64861215 | MS.gene29652:intron | 15.0% |
| !!! | AAGTTTTGATTTGATTTATT+TGG | + | chr5.1:64861572-64861591 | MS.gene29652:intron | 15.0% |
| !!! | CTATAGATTGATTTTATATT+TGG | + | chr5.1:64861847-64861866 | MS.gene29652:intron | 15.0% |
| !! | ACTGGATTTATTATATTTAG+GGG | + | chr5.1:64861198-64861217 | MS.gene29652:intron | 20.0% |
| !! | TTACATATAAACAAAACACA+CGG | - | chr5.1:64861757-64861776 | None:intergenic | 20.0% |
| ! | AAAATAATTCTGATGAGATG+AGG | - | chr5.1:64861178-64861197 | None:intergenic | 25.0% |
| ! | AAGTGAGAATAATAAAGACA+AGG | - | chr5.1:64861997-64862016 | None:intergenic | 25.0% |
| ! | AATGAAACAAACAGGAAAAT+AGG | - | chr5.1:64861733-64861752 | None:intergenic | 25.0% |
| ! | GTTAATTCGGAAAAATTCAA+CGG | + | chr5.1:64861425-64861444 | MS.gene29652:CDS | 25.0% |
| ! | TTGTAATTTAGTCAAACCAT+TGG | + | chr5.1:64861517-64861536 | MS.gene29652:intron | 25.0% |
| !! | AAAATCTGGAACTTAACTAA+CGG | - | chr5.1:64860936-64860955 | None:intergenic | 25.0% |
| !! | CTTAAGAAGTGTAATTATGA+AGG | + | chr5.1:64861363-64861382 | MS.gene29652:intron | 25.0% |
| !! | GTCTTTATTATTCTCACTTT+TGG | + | chr5.1:64861998-64862017 | MS.gene29652:intron | 25.0% |
| !!! | TCTCATCAGAATTATTTTAC+TGG | + | chr5.1:64861180-64861199 | MS.gene29652:intron | 25.0% |
| AAGTTCGTTCAATCACAAAT+GGG | + | chr5.1:64861141-64861160 | MS.gene29652:intron | 30.0% | |
| ATATTTAGGGGATAGAATGA+AGG | + | chr5.1:64861210-64861229 | MS.gene29652:intron | 30.0% | |
| GTTACTTCTCTCATCTTATA+AGG | + | chr5.1:64861090-64861109 | MS.gene29652:CDS | 30.0% | |
| TGAACAAACATGATGAAAAG+AGG | - | chr5.1:64861122-64861141 | None:intergenic | 30.0% | |
| ! | AAATCAACAAAATCTGGAAC+AGG | - | chr5.1:64860993-64861012 | None:intergenic | 30.0% |
| ! | AAATCTGGAACTTAACTAAC+GGG | - | chr5.1:64860935-64860954 | None:intergenic | 30.0% |
| ! | ACTTCTTAAGCATCTTTTCA+AGG | - | chr5.1:64861353-64861372 | None:intergenic | 30.0% |
| ! | GAAGGTGATTCTTATGTTTT+GGG | + | chr5.1:64861228-64861247 | MS.gene29652:CDS | 30.0% |
| ! | GGTTCAATTTCATTGATGAT+GGG | - | chr5.1:64860913-64860932 | None:intergenic | 30.0% |
| ! | GTTAAGTTCCAGATTTTCAA+TGG | + | chr5.1:64860939-64860958 | MS.gene29652:intron | 30.0% |
| ! | TGAAGGTGATTCTTATGTTT+TGG | + | chr5.1:64861227-64861246 | MS.gene29652:CDS | 30.0% |
| ACAAGCAGCAAATTCAATCA+AGG | - | chr5.1:64861686-64861705 | None:intergenic | 35.0% | |
| ACGCGAAAATCAACAAAATC+TGG | - | chr5.1:64860999-64861018 | None:intergenic | 35.0% | |
| AGGAATGTGAAAGACAAAAG+TGG | - | chr5.1:64861977-64861996 | None:intergenic | 35.0% | |
| ATTGTGACGATTATCGATGT+AGG | + | chr5.1:64861398-64861417 | MS.gene29652:intron | 35.0% | |
| CAAGTTCGTTCAATCACAAA+TGG | + | chr5.1:64861140-64861159 | MS.gene29652:intron | 35.0% | |
| CATTTCCCTCATCAACATAT+CGG | + | chr5.1:64861922-64861941 | MS.gene29652:CDS | 35.0% | |
| GATGCTAATGTTATCACTGT+TGG | + | chr5.1:64861449-64861468 | MS.gene29652:CDS | 35.0% | |
| GGGTTCAATTTCATTGATGA+TGG | - | chr5.1:64860914-64860933 | None:intergenic | 35.0% | |
| ! | AATCTGGAACTTAACTAACG+GGG | - | chr5.1:64860934-64860953 | None:intergenic | 35.0% |
| !! | TCTCACTTTTGGTTAAATGC+AGG | + | chr5.1:64862009-64862028 | MS.gene29652:intron | 35.0% |
| !!! | ATTCTTATGTTTTGGGAGGT+AGG | + | chr5.1:64861235-64861254 | MS.gene29652:intron | 35.0% |
| AACTGGAACAGGAACAACAA+AGG | - | chr5.1:64861045-64861064 | None:intergenic | 40.0% | |
| AATGTTATCACTGTTGGTGC+TGG | + | chr5.1:64861455-64861474 | MS.gene29652:CDS | 40.0% | |
| AGAGATGAACTTGGATCCAA+TGG | - | chr5.1:64861536-64861555 | None:intergenic | 40.0% | |
| ATTGCTCTGGTTGTACTCTT+GGG | + | chr5.1:64861488-64861507 | MS.gene29652:CDS | 40.0% | |
| CAAGAATTTGCCATCCAACA+TGG | + | chr5.1:64861319-64861338 | MS.gene29652:intron | 40.0% | |
| CACACGGAAATGAAACAAAC+AGG | - | chr5.1:64861741-64861760 | None:intergenic | 40.0% | |
| CATGCAACAAGAGATGAACT+TGG | - | chr5.1:64861545-64861564 | None:intergenic | 40.0% | |
| CGATGTAGGCGAAGTTAATT+CGG | + | chr5.1:64861412-64861431 | MS.gene29652:intron | 40.0% | |
| GAACAGGACCATTGAAAATC+TGG | - | chr5.1:64860950-64860969 | None:intergenic | 40.0% | |
| GCAGTTATCAACATCTTCGT+TGG | + | chr5.1:64861885-64861904 | MS.gene29652:intron | 40.0% | |
| GTTGGATGGCAAATTCTTGT+TGG | - | chr5.1:64861318-64861337 | None:intergenic | 40.0% | |
| TATTGCTCTGGTTGTACTCT+TGG | + | chr5.1:64861487-64861506 | MS.gene29652:CDS | 40.0% | |
| TCAAGGATCTTAAGCCATGT+TGG | - | chr5.1:64861336-64861355 | None:intergenic | 40.0% | |
| TCATCAACATATCGGCATCA+GGG | + | chr5.1:64861930-64861949 | MS.gene29652:CDS | 40.0% | |
| TTGGGAGGTAGGAGTAAAAA+TGG | + | chr5.1:64861246-64861265 | MS.gene29652:intron | 40.0% | |
| ! | AATCTGGAACAGGAACAACA+CGG | - | chr5.1:64860983-64861002 | None:intergenic | 40.0% |
| ! | TGATGCCGATATGTTGATGA+GGG | - | chr5.1:64861930-64861949 | None:intergenic | 40.0% |
| !! | GGTGATTCTTATGTTTTGGG+AGG | + | chr5.1:64861231-64861250 | MS.gene29652:CDS | 40.0% |
| ACAAAGGCGATGAGAAAAGC+TGG | - | chr5.1:64861029-64861048 | None:intergenic | 45.0% | |
| CCAGTTCTTGCTCTTGACAT+CGG | + | chr5.1:64861059-64861078 | MS.gene29652:CDS | 45.0% | |
| CTCATCAACATATCGGCATC+AGG | + | chr5.1:64861929-64861948 | MS.gene29652:CDS | 45.0% | |
| CTGATGCCGATATGTTGATG+AGG | - | chr5.1:64861931-64861950 | None:intergenic | 45.0% | |
| GGATCTTAAGCCATGTTGGA+TGG | - | chr5.1:64861332-64861351 | None:intergenic | 45.0% | |
| TCAAGAGCAAGAACTGGAAC+AGG | - | chr5.1:64861056-64861075 | None:intergenic | 45.0% | |
| ACACGGTGGAAAACTGGAAC+AGG | - | chr5.1:64860966-64860985 | None:intergenic | 50.0% | |
| ATATCGGCATCAGGGCAACT+TGG | + | chr5.1:64861938-64861957 | MS.gene29652:CDS | 50.0% | |
| CCGATGTCAAGAGCAAGAAC+TGG | - | chr5.1:64861062-64861081 | None:intergenic | 50.0% | |
| CTCTGGTTGTACTCTTGGGA+AGG | + | chr5.1:64861492-64861511 | MS.gene29652:CDS | 50.0% | |
| GGAACAACACGGTGGAAAAC+TGG | - | chr5.1:64860972-64860991 | None:intergenic | 50.0% | |
| !! | TGGTGTTGCTGGTATTGCTC+TGG | + | chr5.1:64861475-64861494 | MS.gene29652:CDS | 50.0% |
| CTGGAACAGGAACAACACGG+TGG | - | chr5.1:64860980-64860999 | None:intergenic | 55.0% | |
| !! | ACTGTTGGTGCTGGTGTTGC+TGG | + | chr5.1:64861464-64861483 | MS.gene29652:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 64860898 | 64862044 | 64860898 | ID=MS.gene29652 |
| chr5.1 | mRNA | 64860898 | 64862044 | 64860898 | ID=MS.gene29652.t1;Parent=MS.gene29652 |
| chr5.1 | exon | 64860898 | 64860934 | 64860898 | ID=MS.gene29652.t1.exon1;Parent=MS.gene29652.t1 |
| chr5.1 | CDS | 64860898 | 64860934 | 64860898 | ID=cds.MS.gene29652.t1;Parent=MS.gene29652.t1 |
| chr5.1 | exon | 64861030 | 64861142 | 64861030 | ID=MS.gene29652.t1.exon2;Parent=MS.gene29652.t1 |
| chr5.1 | CDS | 64861030 | 64861142 | 64861030 | ID=cds.MS.gene29652.t1;Parent=MS.gene29652.t1 |
| chr5.1 | exon | 64861225 | 64861252 | 64861225 | ID=MS.gene29652.t1.exon3;Parent=MS.gene29652.t1 |
| chr5.1 | CDS | 64861225 | 64861252 | 64861225 | ID=cds.MS.gene29652.t1;Parent=MS.gene29652.t1 |
| chr5.1 | exon | 64861420 | 64861513 | 64861420 | ID=MS.gene29652.t1.exon4;Parent=MS.gene29652.t1 |
| chr5.1 | CDS | 64861420 | 64861513 | 64861420 | ID=cds.MS.gene29652.t1;Parent=MS.gene29652.t1 |
| chr5.1 | exon | 64861889 | 64861959 | 64861889 | ID=MS.gene29652.t1.exon5;Parent=MS.gene29652.t1 |
| chr5.1 | CDS | 64861889 | 64861959 | 64861889 | ID=cds.MS.gene29652.t1;Parent=MS.gene29652.t1 |
| chr5.1 | exon | 64862031 | 64862044 | 64862031 | ID=MS.gene29652.t1.exon6;Parent=MS.gene29652.t1 |
| chr5.1 | CDS | 64862031 | 64862044 | 64862031 | ID=cds.MS.gene29652.t1;Parent=MS.gene29652.t1 |
| Gene Sequence |
| Protein sequence |
