Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene058438 | 0.800557 | 1.36E-48 | -1.69E-46 |
| MS.gene058438 | MS.gene060910 | 0.949295 | 1.70E-107 | -1.69E-46 |
| MS.gene058438 | MS.gene08889 | 0.809515 | 1.82E-50 | -1.69E-46 |
| MS.gene058438 | MS.gene22313 | 0.973603 | 1.04E-136 | -1.69E-46 |
| MS.gene058438 | MS.gene26426 | 0.821892 | 3.17E-53 | -1.69E-46 |
| MS.gene058438 | MS.gene29652 | 0.986199 | 5.36E-166 | -1.69E-46 |
| MS.gene058438 | MS.gene38261 | 0.802832 | 4.65E-49 | -1.69E-46 |
| MS.gene058438 | MS.gene39222 | 0.814457 | 1.53E-51 | -1.69E-46 |
| MS.gene058438 | MS.gene46447 | 0.813897 | 2.03E-51 | -1.69E-46 |
| MS.gene058438 | MS.gene48757 | 0.812581 | 3.94E-51 | -1.69E-46 |
| MS.gene058438 | MS.gene62306 | 0.995417 | 4.60E-216 | -1.69E-46 |
| MS.gene058438 | MS.gene63281 | 0.800576 | 1.35E-48 | -1.69E-46 |
| MS.gene058438 | MS.gene67107 | 0.808643 | 2.80E-50 | -1.69E-46 |
| MS.gene058438 | MS.gene73925 | 0.800599 | 1.34E-48 | -1.69E-46 |
| MS.gene058438 | MS.gene81285 | 0.802882 | 4.54E-49 | -1.69E-46 |
| MS.gene058438 | MS.gene85229 | 0.91236 | 2.13E-83 | -1.69E-46 |
| MS.gene058438 | MS.gene90965 | 0.802481 | 5.50E-49 | -1.69E-46 |
| MS.gene058438 | MS.gene99648 | 0.800352 | 1.50E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTACTTCTCTCATCTTATA+AGG | 0.247292 | 1.4:-7050256 | MS.gene058438:CDS |
| ACTGTTGGTGCTGGTGTTGC+TGG | 0.278594 | 1.4:-7049882 | MS.gene058438:CDS |
| TGAAGGTGATTCTTGTGTTT+TGG | 0.303850 | 1.4:-7050119 | MS.gene058438:CDS |
| CGATGTAGGCGAAGTTAATT+CGG | 0.329255 | 1.4:-7049934 | MS.gene058438:intron |
| TATTGCTCTGGTTGTACTCT+TGG | 0.344765 | 1.4:-7049859 | MS.gene058438:CDS |
| GGGTTCAATTTCATTGATGA+TGG | 0.393070 | 1.4:+7050435 | None:intergenic |
| CTCATCAACATATCGGCATC+AGG | 0.407565 | 1.4:-7049417 | MS.gene058438:CDS |
| ATTGCTCTGGTTGTACTCTT+GGG | 0.449876 | 1.4:-7049858 | MS.gene058438:CDS |
| TGGTGTTGCTGGTATTGCTC+TGG | 0.454038 | 1.4:-7049871 | MS.gene058438:CDS |
| AATGTTATCACTGTTGGTGC+TGG | 0.466483 | 1.4:-7049891 | MS.gene058438:CDS |
| CATTTCCCTCATCAACATAT+CGG | 0.470848 | 1.4:-7049424 | MS.gene058438:CDS |
| TCAAGAGCAAGAACTGGAAC+AGG | 0.482132 | 1.4:+7050293 | None:intergenic |
| CCAGTTCTTGCTCTTGACAT+CGG | 0.484096 | 1.4:-7050287 | MS.gene058438:CDS |
| GCAGTTATCAACATCTTCGT+TGG | 0.491361 | 1.4:-7049461 | MS.gene058438:intron |
| CTCTGGTTGTACTCTTGGGA+AGG | 0.498339 | 1.4:-7049854 | MS.gene058438:intron |
| CCGATGTCAAGAGCAAGAAC+TGG | 0.503027 | 1.4:+7050287 | None:intergenic |
| TGAACAAACATGATGAAAAG+AGG | 0.506916 | 1.4:+7050227 | None:intergenic |
| GGTTCAATTTCATTGATGAT+GGG | 0.521972 | 1.4:+7050436 | None:intergenic |
| ATATCGGCATCAGGGCAACT+TGG | 0.528755 | 1.4:-7049408 | MS.gene058438:intron |
| ATATTTAGGGGATAGAATGA+AGG | 0.532536 | 1.4:-7050136 | MS.gene058438:intron |
| GATGCTAATGTTATCACTGT+TGG | 0.589755 | 1.4:-7049897 | MS.gene058438:CDS |
| TCATCAACATATCGGCATCA+GGG | 0.604907 | 1.4:-7049416 | MS.gene058438:CDS |
| AACTGGAACAGGAACAACAA+AGG | 0.628523 | 1.4:+7050304 | None:intergenic |
| CTGATGCCGATATGTTGATG+AGG | 0.666580 | 1.4:+7049418 | None:intergenic |
| TGATGCCGATATGTTGATGA+GGG | 0.679239 | 1.4:+7049419 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATAGATTGATTTTATATTT+GGG | - | chr1.4:7050274-7050293 | MS.gene058438:CDS | 10.0% |
| !! | TACTGGATTTATTATATTTA+GGG | - | chr1.4:7049623-7049642 | MS.gene058438:intron | 15.0% |
| !! | TTACTGGATTTATTATATTT+AGG | - | chr1.4:7049622-7049641 | MS.gene058438:intron | 15.0% |
| !!! | AAGTTTTGATTTGATTTATT+TGG | - | chr1.4:7049998-7050017 | MS.gene058438:intron | 15.0% |
| !!! | CTATAGATTGATTTTATATT+TGG | - | chr1.4:7050273-7050292 | MS.gene058438:CDS | 15.0% |
| !! | ACTGGATTTATTATATTTAG+GGG | - | chr1.4:7049624-7049643 | MS.gene058438:intron | 20.0% |
| !! | TTACATATAAACAAAACACA+CGG | + | chr1.4:7050183-7050202 | None:intergenic | 20.0% |
| ! | AAAATAATTCTGATGAGATG+AGG | + | chr1.4:7049604-7049623 | None:intergenic | 25.0% |
| ! | AAGTGAGAATAATAAAGACA+AGG | + | chr1.4:7050423-7050442 | None:intergenic | 25.0% |
| ! | AATGAAACAAACAGGAAAAT+AGG | + | chr1.4:7050159-7050178 | None:intergenic | 25.0% |
| ! | GTTAATTCGGAAAAATTCAA+CGG | - | chr1.4:7049851-7049870 | MS.gene058438:intron | 25.0% |
| ! | TTGTAATTTAGTCAAACCAT+TGG | - | chr1.4:7049943-7049962 | MS.gene058438:intron | 25.0% |
| !! | AAAATCTGGAACTTAACTAA+CGG | + | chr1.4:7049362-7049381 | None:intergenic | 25.0% |
| !! | CTTAAGAAGTGTAATTATGA+AGG | - | chr1.4:7049789-7049808 | MS.gene058438:intron | 25.0% |
| !! | GTCTTTATTATTCTCACTTT+TGG | - | chr1.4:7050424-7050443 | MS.gene058438:intron | 25.0% |
| !!! | TCTCATCAGAATTATTTTAC+TGG | - | chr1.4:7049606-7049625 | MS.gene058438:intron | 25.0% |
| AAGTTCGTTCAATCACAAAT+GGG | - | chr1.4:7049567-7049586 | MS.gene058438:intron | 30.0% | |
| ATATTTAGGGGATAGAATGA+AGG | - | chr1.4:7049636-7049655 | MS.gene058438:intron | 30.0% | |
| GTTACTTCTCTCATCTTATA+AGG | - | chr1.4:7049516-7049535 | MS.gene058438:intron | 30.0% | |
| TGAACAAACATGATGAAAAG+AGG | + | chr1.4:7049548-7049567 | None:intergenic | 30.0% | |
| ! | AAATCAACAAAATCTGGAAC+AGG | + | chr1.4:7049419-7049438 | None:intergenic | 30.0% |
| ! | AAATCTGGAACTTAACTAAC+GGG | + | chr1.4:7049361-7049380 | None:intergenic | 30.0% |
| ! | ACTTCTTAAGCATCTTTTCA+AGG | + | chr1.4:7049779-7049798 | None:intergenic | 30.0% |
| ! | GGTTCAATTTCATTGATGAT+GGG | + | chr1.4:7049339-7049358 | None:intergenic | 30.0% |
| ! | GTTAAGTTCCAGATTTTCAA+TGG | - | chr1.4:7049365-7049384 | MS.gene058438:intron | 30.0% |
| ACAAGCAGCAAATTCAATCA+AGG | + | chr1.4:7050112-7050131 | None:intergenic | 35.0% | |
| ACGCGAAAATCAACAAAATC+TGG | + | chr1.4:7049425-7049444 | None:intergenic | 35.0% | |
| AGGAATGTGAAAGACAAAAG+TGG | + | chr1.4:7050403-7050422 | None:intergenic | 35.0% | |
| ATTGTGACGATTATCGATGT+AGG | - | chr1.4:7049824-7049843 | MS.gene058438:intron | 35.0% | |
| CAAGTTCGTTCAATCACAAA+TGG | - | chr1.4:7049566-7049585 | MS.gene058438:intron | 35.0% | |
| CATTTCCCTCATCAACATAT+CGG | - | chr1.4:7050348-7050367 | MS.gene058438:intron | 35.0% | |
| GATGCTAATGTTATCACTGT+TGG | - | chr1.4:7049875-7049894 | MS.gene058438:CDS | 35.0% | |
| GGGTTCAATTTCATTGATGA+TGG | + | chr1.4:7049340-7049359 | None:intergenic | 35.0% | |
| ! | AATCTGGAACTTAACTAACG+GGG | + | chr1.4:7049360-7049379 | None:intergenic | 35.0% |
| ! | GAAGGTGATTCTTGTGTTTT+GGG | - | chr1.4:7049654-7049673 | MS.gene058438:intron | 35.0% |
| ! | TGAAGGTGATTCTTGTGTTT+TGG | - | chr1.4:7049653-7049672 | MS.gene058438:intron | 35.0% |
| !! | TCTCACTTTTGGTTAAATGC+AGG | - | chr1.4:7050435-7050454 | MS.gene058438:CDS | 35.0% |
| AACTGGAACAGGAACAACAA+AGG | + | chr1.4:7049471-7049490 | None:intergenic | 40.0% | |
| AATGTTATCACTGTTGGTGC+TGG | - | chr1.4:7049881-7049900 | MS.gene058438:CDS | 40.0% | |
| AGAGATGAACTTGGATCCAA+TGG | + | chr1.4:7049962-7049981 | None:intergenic | 40.0% | |
| ATTGCTCTGGTTGTACTCTT+GGG | - | chr1.4:7049914-7049933 | MS.gene058438:CDS | 40.0% | |
| CAAGAATTTGCCATCCAACA+TGG | - | chr1.4:7049745-7049764 | MS.gene058438:intron | 40.0% | |
| CACACGGAAATGAAACAAAC+AGG | + | chr1.4:7050167-7050186 | None:intergenic | 40.0% | |
| CATGCAACAAGAGATGAACT+TGG | + | chr1.4:7049971-7049990 | None:intergenic | 40.0% | |
| CGATGTAGGCGAAGTTAATT+CGG | - | chr1.4:7049838-7049857 | MS.gene058438:intron | 40.0% | |
| GAACAGGACCATTGAAAATC+TGG | + | chr1.4:7049376-7049395 | None:intergenic | 40.0% | |
| GCAGTTATCAACATCTTCGT+TGG | - | chr1.4:7050311-7050330 | MS.gene058438:CDS | 40.0% | |
| GTTGGATGGCAAATTCTTGT+TGG | + | chr1.4:7049744-7049763 | None:intergenic | 40.0% | |
| TATTGCTCTGGTTGTACTCT+TGG | - | chr1.4:7049913-7049932 | MS.gene058438:CDS | 40.0% | |
| TCAAGGATCTTAAGCCATGT+TGG | + | chr1.4:7049762-7049781 | None:intergenic | 40.0% | |
| TCATCAACATATCGGCATCA+GGG | - | chr1.4:7050356-7050375 | MS.gene058438:intron | 40.0% | |
| TTGGGAGGTAGGAGTAAAAA+TGG | - | chr1.4:7049672-7049691 | MS.gene058438:intron | 40.0% | |
| ! | AATCTGGAACAGGAACAACA+CGG | + | chr1.4:7049409-7049428 | None:intergenic | 40.0% |
| ! | TGATGCCGATATGTTGATGA+GGG | + | chr1.4:7050356-7050375 | None:intergenic | 40.0% |
| !!! | ATTCTTGTGTTTTGGGAGGT+AGG | - | chr1.4:7049661-7049680 | MS.gene058438:intron | 40.0% |
| ACAAAGGCGATGAGAAAAGC+TGG | + | chr1.4:7049455-7049474 | None:intergenic | 45.0% | |
| CCAGTTCTTGCTCTTGACAT+CGG | - | chr1.4:7049485-7049504 | MS.gene058438:intron | 45.0% | |
| CTCATCAACATATCGGCATC+AGG | - | chr1.4:7050355-7050374 | MS.gene058438:intron | 45.0% | |
| CTGATGCCGATATGTTGATG+AGG | + | chr1.4:7050357-7050376 | None:intergenic | 45.0% | |
| GGATCTTAAGCCATGTTGGA+TGG | + | chr1.4:7049758-7049777 | None:intergenic | 45.0% | |
| TCAAGAGCAAGAACTGGAAC+AGG | + | chr1.4:7049482-7049501 | None:intergenic | 45.0% | |
| !! | GGTGATTCTTGTGTTTTGGG+AGG | - | chr1.4:7049657-7049676 | MS.gene058438:intron | 45.0% |
| ACACGGTGGAAAACTGGAAC+AGG | + | chr1.4:7049392-7049411 | None:intergenic | 50.0% | |
| ATATCGGCATCAGGGCAACT+TGG | - | chr1.4:7050364-7050383 | MS.gene058438:intron | 50.0% | |
| CCGATGTCAAGAGCAAGAAC+TGG | + | chr1.4:7049488-7049507 | None:intergenic | 50.0% | |
| CTCTGGTTGTACTCTTGGGA+AGG | - | chr1.4:7049918-7049937 | MS.gene058438:CDS | 50.0% | |
| GGAACAACACGGTGGAAAAC+TGG | + | chr1.4:7049398-7049417 | None:intergenic | 50.0% | |
| !! | TGGTGTTGCTGGTATTGCTC+TGG | - | chr1.4:7049901-7049920 | MS.gene058438:CDS | 50.0% |
| CTGGAACAGGAACAACACGG+TGG | + | chr1.4:7049406-7049425 | None:intergenic | 55.0% | |
| !! | ACTGTTGGTGCTGGTGTTGC+TGG | - | chr1.4:7049890-7049909 | MS.gene058438:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 7049324 | 7050470 | 7049324 | ID=MS.gene058438 |
| chr1.4 | mRNA | 7049324 | 7050470 | 7049324 | ID=MS.gene058438.t1;Parent=MS.gene058438 |
| chr1.4 | exon | 7050434 | 7050470 | 7050434 | ID=MS.gene058438.t1.exon1;Parent=MS.gene058438.t1 |
| chr1.4 | CDS | 7050434 | 7050470 | 7050434 | ID=cds.MS.gene058438.t1;Parent=MS.gene058438.t1 |
| chr1.4 | exon | 7050226 | 7050338 | 7050226 | ID=MS.gene058438.t1.exon2;Parent=MS.gene058438.t1 |
| chr1.4 | CDS | 7050226 | 7050338 | 7050226 | ID=cds.MS.gene058438.t1;Parent=MS.gene058438.t1 |
| chr1.4 | exon | 7050116 | 7050143 | 7050116 | ID=MS.gene058438.t1.exon3;Parent=MS.gene058438.t1 |
| chr1.4 | CDS | 7050116 | 7050143 | 7050116 | ID=cds.MS.gene058438.t1;Parent=MS.gene058438.t1 |
| chr1.4 | exon | 7049855 | 7049948 | 7049855 | ID=MS.gene058438.t1.exon4;Parent=MS.gene058438.t1 |
| chr1.4 | CDS | 7049855 | 7049948 | 7049855 | ID=cds.MS.gene058438.t1;Parent=MS.gene058438.t1 |
| chr1.4 | exon | 7049409 | 7049479 | 7049409 | ID=MS.gene058438.t1.exon5;Parent=MS.gene058438.t1 |
| chr1.4 | CDS | 7049409 | 7049479 | 7049409 | ID=cds.MS.gene058438.t1;Parent=MS.gene058438.t1 |
| chr1.4 | exon | 7049324 | 7049337 | 7049324 | ID=MS.gene058438.t1.exon6;Parent=MS.gene058438.t1 |
| chr1.4 | CDS | 7049324 | 7049337 | 7049324 | ID=cds.MS.gene058438.t1;Parent=MS.gene058438.t1 |
| Gene Sequence |
| Protein sequence |
