Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29693.t1 | XP_013470378.1 | 75.9 | 195 | 13 | 1 | 1 | 161 | 44 | 238 | 2.20E-70 | 275 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29693.t1 | Q39056 | 61.7 | 154 | 25 | 1 | 39 | 158 | 115 | 268 | 1.8e-41 | 170.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29693.t1 | B7FLR3 | 75.9 | 195 | 13 | 1 | 1 | 161 | 44 | 238 | 1.6e-70 | 275.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051710 | MS.gene29693 | 0.801217 | 9.99E-49 | -1.69E-46 |
| MS.gene058515 | MS.gene29693 | 0.80321 | 3.89E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29693.t1 | MTR_1g114590 | 75.897 | 195 | 13 | 1 | 1 | 161 | 44 | 238 | 3.19e-101 | 290 |
| MS.gene29693.t1 | MTR_5g015740 | 75.385 | 195 | 14 | 1 | 1 | 161 | 44 | 238 | 7.89e-100 | 287 |
| MS.gene29693.t1 | MTR_5g015730 | 92.308 | 39 | 3 | 0 | 123 | 161 | 60 | 98 | 9.27e-20 | 79.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29693.t1 | AT1G01290 | 61.688 | 154 | 25 | 1 | 39 | 158 | 115 | 268 | 9.44e-57 | 179 |
| MS.gene29693.t1 | AT1G01290 | 61.688 | 154 | 25 | 1 | 39 | 158 | 115 | 268 | 9.44e-57 | 179 |
Find 35 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAAATGGAATCTGTATT+TGG | 0.170446 | 5.3:-67487178 | None:intergenic |
| GTTCTCTTGCTACTTTCTTT+TGG | 0.215531 | 5.3:+67487007 | None:intergenic |
| TTGACTCATACTGGAAATTC+AGG | 0.267783 | 5.3:-67487055 | MS.gene29693:CDS |
| AAGAAATGGAATCTGTATTT+GGG | 0.311719 | 5.3:-67487177 | None:intergenic |
| GACTTCAGTGTAACAATAGA+AGG | 0.351874 | 5.3:-67486785 | MS.gene29693:CDS |
| TCAAAATCATTATTGTTTCC+AGG | 0.353708 | 5.3:+67486598 | None:intergenic |
| CTCACAGTGGCGAAAATTGC+TGG | 0.376242 | 5.3:-67486902 | MS.gene29693:intron |
| ATTAGACTTAAGCATAAAAC+TGG | 0.405140 | 5.3:-67486641 | MS.gene29693:CDS |
| AATCCACTTGCACGTGTGTA+AGG | 0.416239 | 5.3:+67486825 | None:intergenic |
| ATGGCATCAGTGTCTATTGC+TGG | 0.423752 | 5.3:-67486719 | MS.gene29693:CDS |
| TGTTATGGCACAATGGAATC+AGG | 0.423838 | 5.3:+67486852 | None:intergenic |
| GAAGGGGAAGCTGCTTCAAC+CGG | 0.424889 | 5.3:-67486767 | MS.gene29693:CDS |
| CTATGTTTCGACATGACAAA+TGG | 0.441091 | 5.3:+67487095 | None:intergenic |
| TATGTTTCGACATGACAAAT+GGG | 0.465395 | 5.3:+67487096 | None:intergenic |
| ACAGAGCCAGCTAGTCCATC+TGG | 0.477776 | 5.3:+67487145 | None:intergenic |
| AAATTCAGGGGAAGCTCAAA+TGG | 0.480022 | 5.3:-67487041 | MS.gene29693:CDS |
| ATGTGCAAAGCTGCTTCAAA+AGG | 0.492586 | 5.3:-67486680 | MS.gene29693:CDS |
| TTTGGGGAACTTCCGCCAGA+TGG | 0.539226 | 5.3:-67487160 | MS.gene29693:CDS |
| CTTCCGCCAGATGGACTAGC+TGG | 0.541756 | 5.3:-67487151 | MS.gene29693:CDS |
| ATTCCATTGTGCCATAACAT+AGG | 0.562881 | 5.3:-67486848 | MS.gene29693:CDS |
| CATTGCTTCCATCTCAACCC+CGG | 0.567098 | 5.3:+67486739 | None:intergenic |
| CATTTGTCATGTCGAAACAT+AGG | 0.568946 | 5.3:-67487094 | MS.gene29693:CDS |
| ACTTCAGTGTAACAATAGAA+GGG | 0.575457 | 5.3:-67486784 | MS.gene29693:CDS |
| AGGCCTTACACACGTGCAAG+TGG | 0.575488 | 5.3:-67486828 | MS.gene29693:CDS |
| CGGGGTTGAGATGGAAGCAA+TGG | 0.576890 | 5.3:-67486738 | MS.gene29693:CDS |
| CGTGTGTAAGGCCTATGTTA+TGG | 0.577316 | 5.3:+67486837 | None:intergenic |
| TGACTCATACTGGAAATTCA+GGG | 0.590004 | 5.3:-67487054 | MS.gene29693:CDS |
| AGACTTAAGCATAAAACTGG+TGG | 0.628630 | 5.3:-67486638 | MS.gene29693:CDS |
| CTTCAGTGTAACAATAGAAG+GGG | 0.630495 | 5.3:-67486783 | MS.gene29693:CDS |
| GACTCATACTGGAAATTCAG+GGG | 0.640275 | 5.3:-67487053 | MS.gene29693:CDS |
| GGATTTGAGATTGAATCATG+AGG | 0.642472 | 5.3:-67486807 | MS.gene29693:CDS |
| AGAAATGGAATCTGTATTTG+GGG | 0.645520 | 5.3:-67487176 | None:intergenic |
| AGGCCTATGTTATGGCACAA+TGG | 0.661858 | 5.3:+67486845 | None:intergenic |
| ATGTTTCGACATGACAAATG+GGG | 0.681732 | 5.3:+67487097 | None:intergenic |
| GAGCCAGCTAGTCCATCTGG+CGG | 0.697985 | 5.3:+67487148 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTAGACTTAAGCATAAAAC+TGG | - | chr5.3:67487138-67487157 | MS.gene29693:CDS | 25.0% |
| ACTTCAGTGTAACAATAGAA+GGG | - | chr5.3:67486995-67487014 | MS.gene29693:intron | 30.0% | |
| ATAAAACTGGTGGAAAAAGT+GGG | - | chr5.3:67487151-67487170 | MS.gene29693:CDS | 30.0% | |
| CAAAGTAAATCTTGGAAAGA+AGG | - | chr5.3:67486810-67486829 | MS.gene29693:CDS | 30.0% | |
| TATGTTTCGACATGACAAAT+GGG | + | chr5.3:67486686-67486705 | None:intergenic | 30.0% | |
| TTTGGTATTAGCCAATCAAA+TGG | - | chr5.3:67486840-67486859 | MS.gene29693:CDS | 30.0% | |
| !! | GTCTCATTTTTCCATTTGAT+TGG | + | chr5.3:67486854-67486873 | None:intergenic | 30.0% |
| !! | TCTTTTGAATTGACTCATAC+TGG | - | chr5.3:67486715-67486734 | MS.gene29693:CDS | 30.0% |
| AGACTTAAGCATAAAACTGG+TGG | - | chr5.3:67487141-67487160 | MS.gene29693:CDS | 35.0% | |
| ATGTTTCGACATGACAAATG+GGG | + | chr5.3:67486685-67486704 | None:intergenic | 35.0% | |
| ATTCCATTGTGCCATAACAT+AGG | - | chr5.3:67486931-67486950 | MS.gene29693:intron | 35.0% | |
| ATTTACTTTGCAAACAGCAG+TGG | + | chr5.3:67486800-67486819 | None:intergenic | 35.0% | |
| CATAAAACTGGTGGAAAAAG+TGG | - | chr5.3:67487150-67487169 | MS.gene29693:CDS | 35.0% | |
| CATTTGTCATGTCGAAACAT+AGG | - | chr5.3:67486685-67486704 | MS.gene29693:CDS | 35.0% | |
| CTATGTTTCGACATGACAAA+TGG | + | chr5.3:67486687-67486706 | None:intergenic | 35.0% | |
| CTTCAGTGTAACAATAGAAG+GGG | - | chr5.3:67486996-67487015 | MS.gene29693:intron | 35.0% | |
| GACTTCAGTGTAACAATAGA+AGG | - | chr5.3:67486994-67487013 | MS.gene29693:intron | 35.0% | |
| GCTGTTTGCAAAGTAAATCT+TGG | - | chr5.3:67486802-67486821 | MS.gene29693:CDS | 35.0% | |
| TAAAACTGGTGGAAAAAGTG+GGG | - | chr5.3:67487152-67487171 | MS.gene29693:CDS | 35.0% | |
| TGACTCATACTGGAAATTCA+GGG | - | chr5.3:67486725-67486744 | MS.gene29693:CDS | 35.0% | |
| TTGACTCATACTGGAAATTC+AGG | - | chr5.3:67486724-67486743 | MS.gene29693:CDS | 35.0% | |
| ! | GGATTTGAGATTGAATCATG+AGG | - | chr5.3:67486972-67486991 | MS.gene29693:intron | 35.0% |
| !! | TGGAAAGAAGGTGTTTGATT+TGG | - | chr5.3:67486822-67486841 | MS.gene29693:CDS | 35.0% |
| !!! | GTTCTCTTGCTACTTTCTTT+TGG | + | chr5.3:67486775-67486794 | None:intergenic | 35.0% |
| AAATTCAGGGGAAGCTCAAA+TGG | - | chr5.3:67486738-67486757 | MS.gene29693:CDS | 40.0% | |
| ATGTGCAAAGCTGCTTCAAA+AGG | - | chr5.3:67487099-67487118 | MS.gene29693:CDS | 40.0% | |
| GACTCATACTGGAAATTCAG+GGG | - | chr5.3:67486726-67486745 | MS.gene29693:CDS | 40.0% | |
| TGTTATGGCACAATGGAATC+AGG | + | chr5.3:67486930-67486949 | None:intergenic | 40.0% | |
| AAAATGAGACGTGCTCACAG+TGG | - | chr5.3:67486864-67486883 | MS.gene29693:CDS | 45.0% | |
| AATCCACTTGCACGTGTGTA+AGG | + | chr5.3:67486957-67486976 | None:intergenic | 45.0% | |
| AGGCCTATGTTATGGCACAA+TGG | + | chr5.3:67486937-67486956 | None:intergenic | 45.0% | |
| ATGGCATCAGTGTCTATTGC+TGG | - | chr5.3:67487060-67487079 | MS.gene29693:CDS | 45.0% | |
| CGTGTGTAAGGCCTATGTTA+TGG | + | chr5.3:67486945-67486964 | None:intergenic | 45.0% | |
| GAAAAAGTGGGGATTACTCC+TGG | - | chr5.3:67487163-67487182 | MS.gene29693:CDS | 45.0% | |
| CATTGCTTCCATCTCAACCC+CGG | + | chr5.3:67487043-67487062 | None:intergenic | 50.0% | |
| CTCACAGTGGCGAAAATTGC+TGG | - | chr5.3:67486877-67486896 | MS.gene29693:CDS | 50.0% | |
| CTGCTTCAACCGGAAAAACC+GGG | - | chr5.3:67487022-67487041 | MS.gene29693:CDS | 50.0% | |
| GCTGCTTCAACCGGAAAAAC+CGG | - | chr5.3:67487021-67487040 | MS.gene29693:CDS | 50.0% | |
| TGCTTCAACCGGAAAAACCG+GGG | - | chr5.3:67487023-67487042 | MS.gene29693:CDS | 50.0% | |
| ! | CATCTCAACCCCGGTTTTTC+CGG | + | chr5.3:67487034-67487053 | None:intergenic | 50.0% |
| ACAGAGCCAGCTAGTCCATC+TGG | + | chr5.3:67486637-67486656 | None:intergenic | 55.0% | |
| AGGCCTTACACACGTGCAAG+TGG | - | chr5.3:67486951-67486970 | MS.gene29693:intron | 55.0% | |
| CGGAAAAACCGGGGTTGAGA+TGG | - | chr5.3:67487032-67487051 | MS.gene29693:CDS | 55.0% | |
| CGGGGTTGAGATGGAAGCAA+TGG | - | chr5.3:67487041-67487060 | MS.gene29693:CDS | 55.0% | |
| GAAGGGGAAGCTGCTTCAAC+CGG | - | chr5.3:67487012-67487031 | MS.gene29693:intron | 55.0% | |
| TTTGGGGAACTTCCGCCAGA+TGG | - | chr5.3:67486619-67486638 | MS.gene29693:CDS | 55.0% | |
| GAGCCAGCTAGTCCATCTGG+CGG | + | chr5.3:67486634-67486653 | None:intergenic | 60.0% | |
| !! | CTTCCGCCAGATGGACTAGC+TGG | - | chr5.3:67486628-67486647 | MS.gene29693:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 67486607 | 67487194 | 67486607 | ID=MS.gene29693 |
| chr5.3 | mRNA | 67486607 | 67487194 | 67486607 | ID=MS.gene29693.t1;Parent=MS.gene29693 |
| chr5.3 | exon | 67487020 | 67487194 | 67487020 | ID=MS.gene29693.t1.exon1;Parent=MS.gene29693.t1 |
| chr5.3 | CDS | 67487020 | 67487194 | 67487020 | ID=cds.MS.gene29693.t1;Parent=MS.gene29693.t1 |
| chr5.3 | exon | 67486607 | 67486917 | 67486607 | ID=MS.gene29693.t1.exon2;Parent=MS.gene29693.t1 |
| chr5.3 | CDS | 67486607 | 67486917 | 67486607 | ID=cds.MS.gene29693.t1;Parent=MS.gene29693.t1 |
| Gene Sequence |
| Protein sequence |
