Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29757.t1 | XP_003616828.1 | 98.6 | 357 | 5 | 0 | 1 | 357 | 1 | 357 | 7.50E-196 | 693 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29757.t1 | Q8L633 | 67.0 | 355 | 106 | 3 | 13 | 357 | 1 | 354 | 8.8e-137 | 488.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29757.t1 | G7K7F6 | 98.6 | 357 | 5 | 0 | 1 | 357 | 1 | 357 | 5.4e-196 | 693.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051171 | MS.gene29757 | 0.807365 | 5.24E-50 | -1.69E-46 |
| MS.gene051244 | MS.gene29757 | 0.81976 | 9.81E-53 | -1.69E-46 |
| MS.gene055971 | MS.gene29757 | 0.86344 | 2.49E-64 | -1.69E-46 |
| MS.gene056664 | MS.gene29757 | 0.82204 | 2.93E-53 | -1.69E-46 |
| MS.gene058276 | MS.gene29757 | 0.826045 | 3.36E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29757.t1 | MTR_5g084700 | 98.880 | 357 | 4 | 0 | 1 | 357 | 1 | 357 | 0.0 | 732 |
| MS.gene29757.t1 | MTR_5g093920 | 55.556 | 288 | 128 | 0 | 65 | 352 | 6 | 293 | 1.29e-124 | 360 |
| MS.gene29757.t1 | MTR_5g093920 | 55.556 | 288 | 128 | 0 | 65 | 352 | 29 | 316 | 3.10e-124 | 360 |
| MS.gene29757.t1 | MTR_1g033550 | 50.765 | 327 | 147 | 1 | 26 | 352 | 23 | 335 | 9.89e-123 | 357 |
| MS.gene29757.t1 | MTR_5g093920 | 57.143 | 217 | 93 | 0 | 65 | 281 | 6 | 222 | 2.34e-94 | 281 |
| MS.gene29757.t1 | MTR_1g033550 | 51.172 | 256 | 111 | 1 | 26 | 281 | 23 | 264 | 2.09e-93 | 281 |
| MS.gene29757.t1 | MTR_5g093930 | 47.297 | 74 | 39 | 0 | 71 | 144 | 17 | 90 | 8.75e-21 | 86.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29757.t1 | AT2G04530 | 68.451 | 355 | 101 | 3 | 13 | 357 | 1 | 354 | 0.0 | 520 |
| MS.gene29757.t1 | AT1G74700 | 58.633 | 278 | 115 | 0 | 75 | 352 | 3 | 280 | 2.13e-124 | 359 |
Find 78 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAAATAACCTGACCCTTTA+AGG | 0.057296 | 5.3:-68483134 | None:intergenic |
| TTCCCAACCTATTCCCTTAA+AGG | 0.155367 | 5.3:+68483120 | MS.gene29757:CDS |
| GTCATGTTACCTCAGATATA+TGG | 0.248785 | 5.3:-68491298 | None:intergenic |
| TCTCAATTTGTTTCCCGTTC+AGG | 0.297798 | 5.3:-68490627 | None:intergenic |
| GAGGGAATTGTGTGGTGAAA+TGG | 0.360190 | 5.3:-68483078 | None:intergenic |
| TCTCCTGAGGTAGCCTTCAC+TGG | 0.374275 | 5.3:+68490762 | MS.gene29757:CDS |
| GGGAAGGAAAGTTGTTGTTT+TGG | 0.377652 | 5.3:-68483102 | None:intergenic |
| AAATTGTGAATTGGTTGCTT+TGG | 0.386759 | 5.3:+68489768 | MS.gene29757:CDS |
| CTCAATGCATCGGCATTAAG+TGG | 0.394913 | 5.3:-68490813 | None:intergenic |
| TCCCAACCTATTCCCTTAAA+GGG | 0.404038 | 5.3:+68483121 | MS.gene29757:CDS |
| AGGGAATTGTGTGGTGAAAT+GGG | 0.407490 | 5.3:-68483077 | None:intergenic |
| AACAATACAGTCACCTGAAC+GGG | 0.409727 | 5.3:+68490614 | MS.gene29757:CDS |
| ACCTATTCCCTTAAAGGGTC+AGG | 0.411736 | 5.3:+68483126 | MS.gene29757:CDS |
| AGTTGAACTAAATTGTGAAT+TGG | 0.422079 | 5.3:+68489759 | MS.gene29757:CDS |
| ACCTGACCCTTTAAGGGAAT+AGG | 0.425878 | 5.3:-68483127 | None:intergenic |
| TCAATTGAAGGAGTTTCAAT+TGG | 0.426010 | 5.3:+68483246 | MS.gene29757:CDS |
| TAAATAACCTGACCCTTTAA+GGG | 0.426332 | 5.3:-68483133 | None:intergenic |
| GTTATATAGTCTACTCAATC+AGG | 0.431968 | 5.3:+68490578 | MS.gene29757:CDS |
| ACAGGTTCAACGTAAAGGTT+TGG | 0.435983 | 5.3:+68483209 | MS.gene29757:CDS |
| ACCCTTTAAGGGAATAGGTT+GGG | 0.443580 | 5.3:-68483122 | None:intergenic |
| TTTGCATTTGTGTGGTGAAA+TGG | 0.446678 | 5.3:-68483014 | None:intergenic |
| GGAGGGCTGCCAATGTATAT+AGG | 0.451399 | 5.3:+68489634 | MS.gene29757:CDS |
| CTCCTGAGGTAGCCTTCACT+GGG | 0.453856 | 5.3:+68490763 | MS.gene29757:CDS |
| TTGGAAGGATATTCAATTGA+AGG | 0.462678 | 5.3:+68483234 | MS.gene29757:CDS |
| ATTGAAGGAGTTTCAATTGG+TGG | 0.465522 | 5.3:+68483249 | MS.gene29757:CDS |
| TTCATCAAGATACAATATAG+AGG | 0.465852 | 5.3:+68491699 | MS.gene29757:CDS |
| GATTTGAAGCCTTCTGTAAG+TGG | 0.478640 | 5.3:-68492159 | None:intergenic |
| TGGTGAAATGGGAAAATTTG+TGG | 0.481992 | 5.3:-68483066 | None:intergenic |
| GTCCAAGACTTCAGCAAAAG+TGG | 0.482390 | 5.3:+68492134 | MS.gene29757:CDS |
| CTTGACGTTCACAAGACATT+GGG | 0.483347 | 5.3:+68489733 | MS.gene29757:CDS |
| TCATGTTACCTCAGATATAT+GGG | 0.483425 | 5.3:-68491297 | None:intergenic |
| TCAAATATTGATACCTGGCT+TGG | 0.488765 | 5.3:-68489932 | None:intergenic |
| AAATCACAGGTTCAACGTAA+AGG | 0.499737 | 5.3:+68483204 | MS.gene29757:CDS |
| TTGAAGGTGGGGTTTGTGAG+AGG | 0.509105 | 5.3:-68483039 | None:intergenic |
| CAGGGGAGACATATGAAATA+CGG | 0.510868 | 5.3:+68489867 | MS.gene29757:intron |
| TCAACGTAAAGGTTTGGACT+TGG | 0.516194 | 5.3:+68483215 | MS.gene29757:CDS |
| CTACTGTGGTTGAATCATCT+AGG | 0.522327 | 5.3:-68491250 | None:intergenic |
| TCAACATCGTCTTTGATGCA+AGG | 0.532471 | 5.3:-68489703 | None:intergenic |
| GCTTGACGTTCACAAGACAT+TGG | 0.542921 | 5.3:+68489732 | MS.gene29757:CDS |
| TCCCCAGTGAAGGCTACCTC+AGG | 0.543025 | 5.3:-68490765 | None:intergenic |
| GAATTGGTTGCTTTGGATGT+CGG | 0.545527 | 5.3:+68489775 | MS.gene29757:CDS |
| CGTAAAGGTTTGGACTTGGA+AGG | 0.546281 | 5.3:+68483219 | MS.gene29757:CDS |
| GACCCTTTAAGGGAATAGGT+TGG | 0.555612 | 5.3:-68483123 | None:intergenic |
| ATTCTGATGAATAGCTCTAG+TGG | 0.559629 | 5.3:-68483331 | None:intergenic |
| GGTGAAATGGGAAAATTTGT+GGG | 0.563235 | 5.3:-68483065 | None:intergenic |
| ACAACATGTTATACCAAGCC+AGG | 0.564907 | 5.3:+68489919 | MS.gene29757:CDS |
| ATTCACAATTTAGTTCAACT+TGG | 0.565102 | 5.3:-68489756 | None:intergenic |
| TTCTGATGAATAGCTCTAGT+GGG | 0.572017 | 5.3:-68483330 | None:intergenic |
| TCAAAAGCACATTTAAACTC+AGG | 0.574634 | 5.3:-68483294 | None:intergenic |
| CTTCAAATCACAGTACACAT+AGG | 0.575742 | 5.3:+68492173 | MS.gene29757:CDS |
| TTTAAGGGAATAGGTTGGGA+AGG | 0.580087 | 5.3:-68483118 | None:intergenic |
| ATAACATGTTGTGTTCTGAA+TGG | 0.593217 | 5.3:-68489908 | None:intergenic |
| GCGAAGTGGAAGAGTTGCCA+TGG | 0.593878 | 5.3:-68482992 | None:intergenic |
| CCACGACTTCCTATATACAT+TGG | 0.595400 | 5.3:-68489643 | None:intergenic |
| AAAGTGGTTCCACTTACAGA+AGG | 0.615621 | 5.3:+68492150 | MS.gene29757:CDS |
| AAACAATACAGTCACCTGAA+CGG | 0.618544 | 5.3:+68490613 | MS.gene29757:CDS |
| TGATGTTGTATCCCCAGTGA+AGG | 0.625436 | 5.3:-68490775 | None:intergenic |
| GAGAGGAATTTGCATTTGTG+TGG | 0.626798 | 5.3:-68483022 | None:intergenic |
| CAAGGAGGTACAAATACTGT+GGG | 0.627100 | 5.3:-68489685 | None:intergenic |
| GTACCGTGTTGCGAAATCAC+AGG | 0.631323 | 5.3:+68483191 | MS.gene29757:CDS |
| GAACCTGTGATTTCGCAACA+CGG | 0.631344 | 5.3:-68483194 | None:intergenic |
| TTCTGAATGGTCGAACAACA+AGG | 0.634897 | 5.3:-68489895 | None:intergenic |
| TCAATGCATCGGCATTAAGT+GGG | 0.635067 | 5.3:-68490812 | None:intergenic |
| GGAGGTACAAATACTGTGGG+AGG | 0.640450 | 5.3:-68489682 | None:intergenic |
| GCAAGGAGGTACAAATACTG+TGG | 0.640908 | 5.3:-68489686 | None:intergenic |
| CCAATGTATATAGGAAGTCG+TGG | 0.651899 | 5.3:+68489643 | MS.gene29757:CDS |
| GTGAAATGGGAAAATTTGTG+GGG | 0.659190 | 5.3:-68483064 | None:intergenic |
| TGGTCATACCCATATATCTG+AGG | 0.662316 | 5.3:+68491289 | MS.gene29757:CDS |
| ATATATGGGTATGACCATGT+TGG | 0.662736 | 5.3:-68491283 | None:intergenic |
| AGCAAAAGTTCTTATAACCG+AGG | 0.665868 | 5.3:+68490836 | MS.gene29757:CDS |
| GTAGAGCATGCACGCCAACA+TGG | 0.668020 | 5.3:+68491269 | MS.gene29757:CDS |
| TGAAATGGGAAAATTTGTGG+GGG | 0.677462 | 5.3:-68483063 | None:intergenic |
| TTGGCGTGCATGCTCTACTG+TGG | 0.686614 | 5.3:-68491264 | None:intergenic |
| AGCTCATTGAAAATGCTCAG+TGG | 0.703675 | 5.3:+68491644 | MS.gene29757:intron |
| AGACACTATATTATCTCCTG+AGG | 0.717343 | 5.3:+68490749 | MS.gene29757:CDS |
| TGTGTGGTGAAATGGCGAAG+TGG | 0.729326 | 5.3:-68483006 | None:intergenic |
| ACATCGTCTTTGATGCAAGG+AGG | 0.740269 | 5.3:-68489700 | None:intergenic |
| TCCTGAGGTAGCCTTCACTG+GGG | 0.776935 | 5.3:+68490764 | MS.gene29757:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 68482994 | 68492194 | 68482994 | ID=MS.gene29757 |
| chr5.3 | mRNA | 68482994 | 68492194 | 68482994 | ID=MS.gene29757.t1;Parent=MS.gene29757 |
| chr5.3 | exon | 68482994 | 68483389 | 68482994 | ID=MS.gene29757.t1.exon1;Parent=MS.gene29757.t1 |
| chr5.3 | CDS | 68482994 | 68483389 | 68482994 | ID=cds.MS.gene29757.t1;Parent=MS.gene29757.t1 |
| chr5.3 | exon | 68489634 | 68489796 | 68489634 | ID=MS.gene29757.t1.exon2;Parent=MS.gene29757.t1 |
| chr5.3 | CDS | 68489634 | 68489796 | 68489634 | ID=cds.MS.gene29757.t1;Parent=MS.gene29757.t1 |
| chr5.3 | exon | 68489870 | 68489940 | 68489870 | ID=MS.gene29757.t1.exon3;Parent=MS.gene29757.t1 |
| chr5.3 | CDS | 68489870 | 68489940 | 68489870 | ID=cds.MS.gene29757.t1;Parent=MS.gene29757.t1 |
| chr5.3 | exon | 68490577 | 68490672 | 68490577 | ID=MS.gene29757.t1.exon4;Parent=MS.gene29757.t1 |
| chr5.3 | CDS | 68490577 | 68490672 | 68490577 | ID=cds.MS.gene29757.t1;Parent=MS.gene29757.t1 |
| chr5.3 | exon | 68490744 | 68490857 | 68490744 | ID=MS.gene29757.t1.exon5;Parent=MS.gene29757.t1 |
| chr5.3 | CDS | 68490744 | 68490857 | 68490744 | ID=cds.MS.gene29757.t1;Parent=MS.gene29757.t1 |
| chr5.3 | exon | 68491242 | 68491310 | 68491242 | ID=MS.gene29757.t1.exon6;Parent=MS.gene29757.t1 |
| chr5.3 | CDS | 68491242 | 68491310 | 68491242 | ID=cds.MS.gene29757.t1;Parent=MS.gene29757.t1 |
| chr5.3 | exon | 68491646 | 68491720 | 68491646 | ID=MS.gene29757.t1.exon7;Parent=MS.gene29757.t1 |
| chr5.3 | CDS | 68491646 | 68491720 | 68491646 | ID=cds.MS.gene29757.t1;Parent=MS.gene29757.t1 |
| chr5.3 | exon | 68492105 | 68492194 | 68492105 | ID=MS.gene29757.t1.exon8;Parent=MS.gene29757.t1 |
| chr5.3 | CDS | 68492105 | 68492194 | 68492105 | ID=cds.MS.gene29757.t1;Parent=MS.gene29757.t1 |
| Gene Sequence |
| Protein sequence |
