Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30304.t1 | KEH22222.1 | 98.6 | 491 | 7 | 0 | 1 | 491 | 1 | 491 | 6.90E-285 | 989.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30304.t1 | F4J117 | 67.4 | 491 | 155 | 4 | 3 | 491 | 64 | 551 | 2.5e-198 | 693.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30304.t1 | A0A072TXQ6 | 98.6 | 491 | 7 | 0 | 1 | 491 | 1 | 491 | 5.0e-285 | 989.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057926 | MS.gene30304 | 0.812227 | 4.71E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30304.t1 | MTR_7g034465 | 98.574 | 491 | 7 | 0 | 1 | 491 | 1 | 491 | 0.0 | 1014 |
| MS.gene30304.t1 | MTR_7g034465 | 97.339 | 451 | 12 | 0 | 1 | 451 | 107 | 557 | 0.0 | 919 |
| MS.gene30304.t1 | MTR_1g090867 | 32.246 | 276 | 167 | 10 | 222 | 489 | 94 | 357 | 4.25e-35 | 135 |
| MS.gene30304.t1 | MTR_3g094300 | 39.773 | 176 | 104 | 2 | 208 | 381 | 72 | 247 | 1.05e-31 | 123 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30304.t1 | AT3G01510 | 66.735 | 490 | 160 | 3 | 3 | 491 | 64 | 551 | 0.0 | 687 |
| MS.gene30304.t1 | AT3G01510 | 65.207 | 411 | 140 | 3 | 82 | 491 | 1 | 409 | 0.0 | 563 |
| MS.gene30304.t1 | AT3G01510 | 65.207 | 411 | 140 | 3 | 82 | 491 | 1 | 409 | 0.0 | 563 |
| MS.gene30304.t1 | AT3G52180 | 31.518 | 257 | 163 | 7 | 209 | 461 | 74 | 321 | 1.90e-34 | 133 |
| MS.gene30304.t1 | AT3G52180 | 31.064 | 235 | 150 | 6 | 230 | 461 | 1 | 226 | 4.53e-31 | 122 |
| MS.gene30304.t1 | AT3G52180 | 35.593 | 177 | 110 | 4 | 209 | 382 | 74 | 249 | 2.90e-28 | 114 |
| MS.gene30304.t1 | AT3G52180 | 35.593 | 177 | 110 | 4 | 209 | 382 | 74 | 249 | 4.70e-28 | 113 |
| MS.gene30304.t1 | AT3G10940 | 34.091 | 176 | 114 | 2 | 208 | 381 | 68 | 243 | 1.47e-27 | 112 |
Find 114 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACTACAAGTTCATTGTTAA+TGG | 0.178065 | 7.4:+65760552 | MS.gene30304:CDS |
| AAGTGGTTCTTTCCAATTTC+CGG | 0.189422 | 7.4:-65760285 | None:intergenic |
| GTGGAACTGAAGCTGAAAAT+TGG | 0.234882 | 7.4:+65755528 | MS.gene30304:CDS |
| TTCAAGTGGAAGCAAGTTAT+TGG | 0.237528 | 7.4:+65754626 | MS.gene30304:CDS |
| AATAAAAGCCCAACACAAAA+TGG | 0.242291 | 7.4:-65756034 | None:intergenic |
| AGTGATAGCGGTGGCAATTC+TGG | 0.262099 | 7.4:+65754573 | MS.gene30304:CDS |
| TTGGAGACTTCACCGGAAAT+TGG | 0.273320 | 7.4:+65760273 | MS.gene30304:CDS |
| CCCTACATGCAGCCCATACT+TGG | 0.290737 | 7.4:+65756161 | MS.gene30304:CDS |
| TCCACTTGAGGAATATATTC+AGG | 0.341950 | 7.4:+65754773 | MS.gene30304:CDS |
| GCATCCCAGTGACCCAAGTA+TGG | 0.349243 | 7.4:-65756174 | None:intergenic |
| TGGAACTGAAGCTGAAAATT+GGG | 0.363423 | 7.4:+65755529 | MS.gene30304:CDS |
| TTTCCACGTCATCTCCTGTT+TGG | 0.364566 | 7.4:-65755007 | None:intergenic |
| CCCCGATGCTTTGCCTTAAG+TGG | 0.366305 | 7.4:-65760302 | None:intergenic |
| TTGAGAAGCCTCTTGGTATT+CGG | 0.368115 | 7.4:+65752287 | MS.gene30304:CDS |
| GCCTTGTTCCATGTGACAAC+AGG | 0.373050 | 7.4:-65754522 | None:intergenic |
| CATGTTGCCCATGCAATTGC+CGG | 0.376920 | 7.4:-65759607 | None:intergenic |
| CGGTTTGCTCTCACTTCTGA+TGG | 0.383362 | 7.4:+65752307 | MS.gene30304:CDS |
| TCAAGTGGAAGCAAGTTATT+GGG | 0.385419 | 7.4:+65754627 | MS.gene30304:CDS |
| ACCACTTAAGGCAAAGCATC+GGG | 0.395885 | 7.4:+65760301 | MS.gene30304:CDS |
| CTTATTATGCAGAAGTTGAA+TGG | 0.400805 | 7.4:-65754460 | None:intergenic |
| GAATACTTTACTTACCCCTT+TGG | 0.401334 | 7.4:-65752353 | None:intergenic |
| TCATCATCAACTATCCTATA+CGG | 0.410245 | 7.4:+65755600 | MS.gene30304:CDS |
| AAGCGAGTAGAGATGGAGAT+TGG | 0.415212 | 7.4:+65754736 | MS.gene30304:CDS |
| GGAGAAGTCCAGGATAATTA+TGG | 0.416680 | 7.4:+65753303 | MS.gene30304:CDS |
| TGGACCCTAGATTACCGTAT+AGG | 0.418003 | 7.4:-65755614 | None:intergenic |
| CGGCAATTGCATGGGCAACA+TGG | 0.433325 | 7.4:+65759608 | MS.gene30304:CDS |
| CCAAGTATGGGCTGCATGTA+GGG | 0.447468 | 7.4:-65756161 | None:intergenic |
| GTTAACAATATAATAATGAT+TGG | 0.448260 | 7.4:+65760627 | MS.gene30304:CDS |
| GTCATCTCCTGTTTGGATGC+AGG | 0.449317 | 7.4:-65755000 | None:intergenic |
| GTTACTCTTGAGAAGCCTCT+TGG | 0.450603 | 7.4:+65752280 | MS.gene30304:CDS |
| GCAGCCCATACTTGGGTCAC+TGG | 0.460968 | 7.4:+65756169 | MS.gene30304:CDS |
| ACTGCAGGCCGGCAATTGCA+TGG | 0.461746 | 7.4:+65759599 | MS.gene30304:intron |
| CCGCTATCATCTCTTTCAGC+TGG | 0.465558 | 7.4:-65760600 | None:intergenic |
| GTGGGATCCTGCATCCAAAC+AGG | 0.468080 | 7.4:+65754993 | MS.gene30304:CDS |
| TGCACACGTGCAGGCCTGAC+AGG | 0.474713 | 7.4:+65756194 | MS.gene30304:CDS |
| TTTCCTCAGGAAGGTGCTAA+AGG | 0.475366 | 7.4:+65754386 | MS.gene30304:intron |
| TCTTGGTATGCGTTATAGTA+AGG | 0.480275 | 7.4:+65754836 | MS.gene30304:CDS |
| TTTCAGCTTCAGTTCCACTT+TGG | 0.489822 | 7.4:-65755523 | None:intergenic |
| TCTAAGTTTCTTAATTCAAG+TGG | 0.490787 | 7.4:+65754612 | MS.gene30304:CDS |
| TTGTTCACTCTCTCGCCAAA+GGG | 0.493733 | 7.4:+65752338 | MS.gene30304:CDS |
| TTGCCACCGCTATCACTAGA+TGG | 0.493996 | 7.4:-65754567 | None:intergenic |
| AGCGAGTAGAGATGGAGATT+GGG | 0.494191 | 7.4:+65754737 | MS.gene30304:CDS |
| GATTACCGAGCAAATATATG+TGG | 0.494561 | 7.4:+65754974 | MS.gene30304:intron |
| CCCAAGTATGGGCTGCATGT+AGG | 0.497954 | 7.4:-65756162 | None:intergenic |
| ATAAAAGCCCAACACAAAAT+GGG | 0.499871 | 7.4:-65756033 | None:intergenic |
| ATTTCTTTCACCGTGGGTGA+TGG | 0.504844 | 7.4:-65754667 | None:intergenic |
| GGGGAGGATGTAAATTTGGT+TGG | 0.505086 | 7.4:+65760254 | MS.gene30304:CDS |
| GATCTTATAGCTATGGTTGA+AGG | 0.508873 | 7.4:+65759631 | MS.gene30304:CDS |
| CTGCAGGCCGGCAATTGCAT+GGG | 0.509683 | 7.4:+65759600 | MS.gene30304:intron |
| GCAAGAATAACTAACCTGTC+AGG | 0.512789 | 7.4:-65756208 | None:intergenic |
| AGGGTAATGCGGAGAAGTCC+AGG | 0.513501 | 7.4:+65753293 | MS.gene30304:intron |
| CTTGCACTTCTGGATTTGAT+CGG | 0.516485 | 7.4:+65756092 | MS.gene30304:CDS |
| CAGCCCATACTTGGGTCACT+GGG | 0.519078 | 7.4:+65756170 | MS.gene30304:CDS |
| GGCAATTGCATGGGCAACAT+GGG | 0.519858 | 7.4:+65759609 | MS.gene30304:CDS |
| GTCACCCACCATAATTATCC+TGG | 0.521511 | 7.4:-65753311 | None:intergenic |
| GCCTGAATATATTCCTCAAG+TGG | 0.522255 | 7.4:-65754774 | None:intergenic |
| AGGAGATGACGTGGAAACCT+TGG | 0.524257 | 7.4:+65755013 | MS.gene30304:CDS |
| TTATTATGCAGAAGTTGAAT+GGG | 0.525459 | 7.4:-65754459 | None:intergenic |
| CTTGAAATAGTATATAATAG+AGG | 0.527177 | 7.4:+65754492 | MS.gene30304:CDS |
| GGTGACACTTTGAAGAAAGC+TGG | 0.528329 | 7.4:+65753328 | MS.gene30304:CDS |
| TCACTGGGATGCACACGTGC+AGG | 0.531651 | 7.4:+65756185 | MS.gene30304:CDS |
| AAATTGGAAAGAACCACTTA+AGG | 0.532240 | 7.4:+65760289 | MS.gene30304:CDS |
| ATTGTTCACTCTCTCGCCAA+AGG | 0.532399 | 7.4:+65752337 | MS.gene30304:CDS |
| ATTATAGTACATCTCATCCT+TGG | 0.534194 | 7.4:-65754808 | None:intergenic |
| AACCACTTAAGGCAAAGCAT+CGG | 0.546151 | 7.4:+65760300 | MS.gene30304:CDS |
| ACTGACGAAGCGAGTAGAGA+TGG | 0.549177 | 7.4:+65754729 | MS.gene30304:CDS |
| ATGTACTATAATCACTCTCT+TGG | 0.549559 | 7.4:+65754819 | MS.gene30304:CDS |
| AACATGGGATCTTATAGCTA+TGG | 0.554777 | 7.4:+65759624 | MS.gene30304:CDS |
| CAATCAATGACTATTGTCAA+AGG | 0.562058 | 7.4:+65755567 | MS.gene30304:CDS |
| AATTTGGTTGGAGACTTCAC+CGG | 0.565379 | 7.4:+65760266 | MS.gene30304:CDS |
| GGTTTGCTCTCACTTCTGAT+GGG | 0.567287 | 7.4:+65752308 | MS.gene30304:CDS |
| AGGTATGCAATCACACAAGC+AGG | 0.569325 | 7.4:-65756118 | None:intergenic |
| CCTACATGCAGCCCATACTT+GGG | 0.572114 | 7.4:+65756162 | MS.gene30304:CDS |
| GTCGAGTTCCTGTTGTCACA+TGG | 0.574747 | 7.4:+65754514 | MS.gene30304:CDS |
| GGAAACCTTGGCAAAAGTTG+AGG | 0.576323 | 7.4:+65755025 | MS.gene30304:CDS |
| AGTTCATTGTTAATGGACAA+TGG | 0.577080 | 7.4:+65760559 | MS.gene30304:CDS |
| TGAATGGGAAATGTAGATGC+GGG | 0.577092 | 7.4:-65754444 | None:intergenic |
| TCCTGTTGTCACATGGAACA+AGG | 0.584927 | 7.4:+65754521 | MS.gene30304:CDS |
| AGAGATGGAGATTGGGCACA+CGG | 0.589638 | 7.4:+65754744 | MS.gene30304:CDS |
| AAACGTCTGTCATCCAATGT+AGG | 0.592411 | 7.4:-65756138 | None:intergenic |
| CTAGTTGAGATTAAAGACGT+TGG | 0.600295 | 7.4:+65753370 | MS.gene30304:CDS |
| CACAAATGTGACAGCATGTG+TGG | 0.600778 | 7.4:-65759671 | None:intergenic |
| TAACGAATTTCTTTCACCGT+GGG | 0.604263 | 7.4:-65754673 | None:intergenic |
| TAGCACACATACTTTCCTTG+TGG | 0.605787 | 7.4:-65760356 | None:intergenic |
| GGATCCCACATATATTTGCT+CGG | 0.608187 | 7.4:-65754979 | None:intergenic |
| GAAGTCCAGGATAATTATGG+TGG | 0.610755 | 7.4:+65753306 | MS.gene30304:CDS |
| GCTGTCACATTTGTGTGGAA+TGG | 0.617368 | 7.4:+65759679 | MS.gene30304:CDS |
| GCTGTTCTGAACTTCCAAAG+TGG | 0.617537 | 7.4:+65755509 | MS.gene30304:CDS |
| ATGGAGATTCTTACGACATG+AGG | 0.617731 | 7.4:+65756002 | MS.gene30304:CDS |
| CACATGCTGTCACATTTGTG+TGG | 0.626265 | 7.4:+65759674 | MS.gene30304:CDS |
| CATCCCAGTGACCCAAGTAT+GGG | 0.627109 | 7.4:-65756173 | None:intergenic |
| CATCCAAACAGGAGATGACG+TGG | 0.632871 | 7.4:+65755004 | MS.gene30304:CDS |
| TTGAATGGGAAATGTAGATG+CGG | 0.634352 | 7.4:-65754445 | None:intergenic |
| GGAACTGAAGCTGAAAATTG+GGG | 0.634841 | 7.4:+65755530 | MS.gene30304:CDS |
| CTTATAGCTATGGTTGAAGG+TGG | 0.641208 | 7.4:+65759634 | MS.gene30304:CDS |
| CTGAACAGAAGGACGAACGC+TGG | 0.641353 | 7.4:-65760658 | None:intergenic |
| ATTACCGAGCAAATATATGT+GGG | 0.647709 | 7.4:+65754975 | MS.gene30304:CDS |
| AATCAACATGCCATCACCCA+CGG | 0.660027 | 7.4:+65754657 | MS.gene30304:CDS |
| GTGTGATTGCATACCTACAT+TGG | 0.665139 | 7.4:+65756125 | MS.gene30304:CDS |
| AAGTCCAGGATAATTATGGT+GGG | 0.667424 | 7.4:+65753307 | MS.gene30304:CDS |
| TTACAACCATCTAGTGATAG+CGG | 0.670362 | 7.4:+65754561 | MS.gene30304:CDS |
| TCAGGCTCTAGATCGTGCCA+AGG | 0.672514 | 7.4:+65754791 | MS.gene30304:CDS |
| CTTAAGGCAAAGCATCGGGG+TGG | 0.679304 | 7.4:+65760305 | MS.gene30304:CDS |
| TGTTCACTCTCTCGCCAAAG+GGG | 0.683682 | 7.4:+65752339 | MS.gene30304:CDS |
| CATGTGTGGGAGCATCATGT+TGG | 0.696530 | 7.4:-65759657 | None:intergenic |
| AGAGCAAACCGAATACCAAG+AGG | 0.696988 | 7.4:-65752295 | None:intergenic |
| CAACCATCTAGTGATAGCGG+TGG | 0.697176 | 7.4:+65754564 | MS.gene30304:CDS |
| CTCAACTAGACTATTCTGAG+AGG | 0.697404 | 7.4:-65753356 | None:intergenic |
| ATTTGTGTGGAATGGTCACG+AGG | 0.703652 | 7.4:+65759687 | MS.gene30304:CDS |
| TTAACGAATTTCTTTCACCG+TGG | 0.706941 | 7.4:-65754674 | None:intergenic |
| CCACTTAAGGCAAAGCATCG+GGG | 0.714854 | 7.4:+65760302 | MS.gene30304:CDS |
| ACAAATGTGACAGCATGTGT+GGG | 0.717710 | 7.4:-65759670 | None:intergenic |
| CCAGCTGAAAGAGATGATAG+CGG | 0.721987 | 7.4:+65760600 | MS.gene30304:CDS |
| GTAGAAGTTAAACTTCCACA+AGG | 0.735963 | 7.4:+65760341 | MS.gene30304:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 65752229 | 65760692 | 65752229 | ID=MS.gene30304 |
| chr7.4 | mRNA | 65752229 | 65760692 | 65752229 | ID=MS.gene30304.t1;Parent=MS.gene30304 |
| chr7.4 | exon | 65752229 | 65752360 | 65752229 | ID=MS.gene30304.t1.exon1;Parent=MS.gene30304.t1 |
| chr7.4 | CDS | 65752229 | 65752360 | 65752229 | ID=cds.MS.gene30304.t1;Parent=MS.gene30304.t1 |
| chr7.4 | exon | 65753295 | 65753401 | 65753295 | ID=MS.gene30304.t1.exon2;Parent=MS.gene30304.t1 |
| chr7.4 | CDS | 65753295 | 65753401 | 65753295 | ID=cds.MS.gene30304.t1;Parent=MS.gene30304.t1 |
| chr7.4 | exon | 65754395 | 65754857 | 65754395 | ID=MS.gene30304.t1.exon3;Parent=MS.gene30304.t1 |
| chr7.4 | CDS | 65754395 | 65754857 | 65754395 | ID=cds.MS.gene30304.t1;Parent=MS.gene30304.t1 |
| chr7.4 | exon | 65754975 | 65755046 | 65754975 | ID=MS.gene30304.t1.exon4;Parent=MS.gene30304.t1 |
| chr7.4 | CDS | 65754975 | 65755046 | 65754975 | ID=cds.MS.gene30304.t1;Parent=MS.gene30304.t1 |
| chr7.4 | exon | 65755500 | 65755621 | 65755500 | ID=MS.gene30304.t1.exon5;Parent=MS.gene30304.t1 |
| chr7.4 | CDS | 65755500 | 65755621 | 65755500 | ID=cds.MS.gene30304.t1;Parent=MS.gene30304.t1 |
| chr7.4 | exon | 65756000 | 65756215 | 65756000 | ID=MS.gene30304.t1.exon6;Parent=MS.gene30304.t1 |
| chr7.4 | CDS | 65756000 | 65756215 | 65756000 | ID=cds.MS.gene30304.t1;Parent=MS.gene30304.t1 |
| chr7.4 | exon | 65759606 | 65759708 | 65759606 | ID=MS.gene30304.t1.exon7;Parent=MS.gene30304.t1 |
| chr7.4 | CDS | 65759606 | 65759708 | 65759606 | ID=cds.MS.gene30304.t1;Parent=MS.gene30304.t1 |
| chr7.4 | exon | 65760254 | 65760366 | 65760254 | ID=MS.gene30304.t1.exon8;Parent=MS.gene30304.t1 |
| chr7.4 | CDS | 65760254 | 65760366 | 65760254 | ID=cds.MS.gene30304.t1;Parent=MS.gene30304.t1 |
| chr7.4 | exon | 65760548 | 65760692 | 65760548 | ID=MS.gene30304.t1.exon9;Parent=MS.gene30304.t1 |
| chr7.4 | CDS | 65760548 | 65760692 | 65760548 | ID=cds.MS.gene30304.t1;Parent=MS.gene30304.t1 |
| Gene Sequence |
| Protein sequence |
