Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30313.t1 | XP_024628602.1 | 89.7 | 232 | 24 | 0 | 1 | 232 | 27 | 258 | 3.20E-115 | 424.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30313.t1 | A0A072TM10 | 91.4 | 186 | 16 | 0 | 47 | 232 | 1 | 186 | 3.4e-90 | 340.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050315 | MS.gene30313 | 0.823406 | 1.41E-53 | -1.69E-46 |
| MS.gene052589 | MS.gene30313 | 0.818636 | 1.77E-52 | -1.69E-46 |
| MS.gene054102 | MS.gene30313 | 0.829653 | 4.56E-55 | -1.69E-46 |
| MS.gene054110 | MS.gene30313 | 0.821157 | 4.69E-53 | -1.69E-46 |
| MS.gene056514 | MS.gene30313 | 0.809747 | 1.62E-50 | -1.69E-46 |
| MS.gene057888 | MS.gene30313 | 0.811012 | 8.66E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30313.t1 | MTR_8g019440 | 91.398 | 186 | 16 | 0 | 47 | 232 | 1 | 186 | 1.78e-116 | 330 |
| MS.gene30313.t1 | MTR_8g019060 | 67.580 | 219 | 70 | 1 | 1 | 219 | 44 | 261 | 1.43e-92 | 279 |
| MS.gene30313.t1 | MTR_8g019060 | 68.852 | 183 | 54 | 3 | 29 | 209 | 262 | 443 | 2.41e-72 | 227 |
| MS.gene30313.t1 | MTR_8g019450 | 50.617 | 243 | 100 | 3 | 1 | 226 | 1 | 240 | 5.71e-77 | 233 |
| MS.gene30313.t1 | MTR_8g018980 | 48.963 | 241 | 105 | 2 | 1 | 224 | 1 | 240 | 6.10e-72 | 219 |
| MS.gene30313.t1 | MTR_0470s0020 | 47.718 | 241 | 108 | 2 | 1 | 224 | 1 | 240 | 3.28e-71 | 218 |
| MS.gene30313.t1 | MTR_8g019050 | 47.718 | 241 | 108 | 2 | 1 | 224 | 1 | 240 | 3.28e-71 | 218 |
| MS.gene30313.t1 | MTR_0514s0010 | 48.548 | 241 | 106 | 3 | 1 | 224 | 3 | 242 | 7.84e-71 | 217 |
| MS.gene30313.t1 | MTR_8g019370 | 70.440 | 159 | 45 | 2 | 65 | 222 | 1 | 158 | 2.76e-68 | 207 |
| MS.gene30313.t1 | MTR_8g019380 | 46.473 | 241 | 111 | 6 | 1 | 226 | 1 | 238 | 4.83e-63 | 198 |
| MS.gene30313.t1 | MTR_8g019260 | 47.887 | 142 | 67 | 2 | 38 | 179 | 8 | 142 | 1.10e-37 | 130 |
| MS.gene30313.t1 | MTR_8g019110 | 47.887 | 142 | 67 | 2 | 38 | 179 | 8 | 142 | 1.10e-37 | 130 |
| MS.gene30313.t1 | MTR_8g019290 | 46.479 | 142 | 69 | 2 | 38 | 179 | 8 | 142 | 2.19e-36 | 127 |
| MS.gene30313.t1 | MTR_7g055630 | 45.390 | 141 | 69 | 3 | 42 | 179 | 13 | 148 | 3.78e-32 | 116 |
| MS.gene30313.t1 | MTR_0470s0010 | 43.571 | 140 | 78 | 1 | 38 | 177 | 10 | 148 | 7.15e-31 | 112 |
| MS.gene30313.t1 | MTR_8g019020 | 43.571 | 140 | 78 | 1 | 38 | 177 | 10 | 148 | 7.15e-31 | 112 |
| MS.gene30313.t1 | MTR_8g019280 | 49.153 | 118 | 53 | 2 | 62 | 179 | 2 | 112 | 6.39e-28 | 103 |
| MS.gene30313.t1 | MTR_1g069110 | 31.884 | 207 | 124 | 5 | 6 | 209 | 242 | 434 | 2.80e-22 | 94.7 |
| MS.gene30313.t1 | MTR_7g099570 | 34.682 | 173 | 103 | 4 | 39 | 209 | 424 | 588 | 2.00e-20 | 89.7 |
| MS.gene30313.t1 | MTR_7g087540 | 34.356 | 163 | 92 | 4 | 37 | 197 | 26 | 175 | 2.93e-19 | 82.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30313.t1 | AT3G13950 | 28.977 | 176 | 105 | 6 | 38 | 207 | 24 | 185 | 2.09e-14 | 70.1 |
Find 51 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACACTGCGTATTATTATT+TGG | 0.053800 | 8.3:+71363773 | MS.gene30313:CDS |
| TTTCAACATTGCTTCGTATT+TGG | 0.140918 | 8.3:+71363719 | MS.gene30313:CDS |
| CACACTGCGTATTATTATTT+GGG | 0.201281 | 8.3:+71363774 | MS.gene30313:CDS |
| TTACCGAATAGCAATATTAA+TGG | 0.237697 | 8.3:+71363657 | MS.gene30313:CDS |
| AGGAAGTGAGAATAGTATTC+AGG | 0.264992 | 8.3:-71363496 | None:intergenic |
| TTATCTCCATCATCTTTAAT+AGG | 0.273453 | 8.3:-71363391 | None:intergenic |
| ACCTTTCTCCAACCTTTCTT+TGG | 0.296921 | 8.3:-71363226 | None:intergenic |
| ACCTTTCTTTGGTTCGTCAT+TGG | 0.323590 | 8.3:-71363215 | None:intergenic |
| GTGGAATTCGTTTGAGTCAT+AGG | 0.336965 | 8.3:+71363575 | MS.gene30313:CDS |
| AGCATGTTCTAAAGGTTATA+AGG | 0.340199 | 8.3:+71363429 | MS.gene30313:CDS |
| TTCTTTGGTTCGTCATTGGT+TGG | 0.355017 | 8.3:-71363211 | None:intergenic |
| TCTTTGGTTCGTCATTGGTT+GGG | 0.357553 | 8.3:-71363210 | None:intergenic |
| TTATCACTCCAGACAGGATC+TGG | 0.363394 | 8.3:-71363685 | None:intergenic |
| TAACCATTAATATTGCTATT+CGG | 0.415211 | 8.3:-71363660 | None:intergenic |
| CTTTGGTTCGTCATTGGTTG+GGG | 0.416447 | 8.3:-71363209 | None:intergenic |
| AACATATTGTGTTCCAGAAA+AGG | 0.437742 | 8.3:-71363516 | None:intergenic |
| TGGTTACCCCAGATCCTGTC+TGG | 0.441455 | 8.3:+71363677 | MS.gene30313:CDS |
| ACATTGCTTCGTATTTGGAT+TGG | 0.451577 | 8.3:+71363724 | MS.gene30313:CDS |
| GGAAGTGAGAATAGTATTCA+GGG | 0.452495 | 8.3:-71363495 | None:intergenic |
| CAAGCCGATGCAATCATCAT+TGG | 0.452634 | 8.3:-71363844 | None:intergenic |
| AGCAACAATAACGTTTCAAA+TGG | 0.467865 | 8.3:+71363342 | MS.gene30313:CDS |
| TAAGGACTTGAAGCTATGTC+CGG | 0.473893 | 8.3:+71363447 | MS.gene30313:CDS |
| TAAATCCACCCGGTGGCGTT+AGG | 0.490777 | 8.3:+71363368 | MS.gene30313:CDS |
| ACCAAAGAAAGGTTGGAGAA+AGG | 0.503713 | 8.3:+71363225 | MS.gene30313:CDS |
| AATAATACAGCATGTTCTAA+AGG | 0.504058 | 8.3:+71363421 | MS.gene30313:CDS |
| AACAATCAAGTGGATAATCA+TGG | 0.516221 | 8.3:+71363172 | MS.gene30313:CDS |
| TTTAATAGGCCTAACGCCAC+CGG | 0.516305 | 8.3:-71363377 | None:intergenic |
| TGAGTCATAGGTTTAGTATG+TGG | 0.529830 | 8.3:+71363587 | MS.gene30313:CDS |
| CGAAAACAAGAATGAGTGGT+TGG | 0.531380 | 8.3:+71363279 | MS.gene30313:CDS |
| TATCACTCCAGACAGGATCT+GGG | 0.537086 | 8.3:-71363684 | None:intergenic |
| ACACTGCGTATTATTATTTG+GGG | 0.542002 | 8.3:+71363775 | MS.gene30313:CDS |
| GTTAGGCCTATTAAAGATGA+TGG | 0.542440 | 8.3:+71363385 | MS.gene30313:CDS |
| ACCAATGACGAACCAAAGAA+AGG | 0.542566 | 8.3:+71363214 | MS.gene30313:CDS |
| ATGACGAACCAAAGAAAGGT+TGG | 0.557249 | 8.3:+71363218 | MS.gene30313:CDS |
| TCCTGTCTGGAGTGATAATG+AGG | 0.557744 | 8.3:+71363690 | MS.gene30313:CDS |
| CACTCCAATGATGATTGCAT+CGG | 0.588109 | 8.3:+71363840 | MS.gene30313:CDS |
| AAGGACTTGAAGCTATGTCC+GGG | 0.593407 | 8.3:+71363448 | MS.gene30313:CDS |
| TTAATAGGCCTAACGCCACC+GGG | 0.597506 | 8.3:-71363376 | None:intergenic |
| TGTGAGTAACAAACCCGAGA+AGG | 0.604751 | 8.3:-71363755 | None:intergenic |
| GAGTGGTTGGATCAAATGAG+AGG | 0.610628 | 8.3:+71363292 | MS.gene30313:CDS |
| ACCTCATTATCACTCCAGAC+AGG | 0.612601 | 8.3:-71363691 | None:intergenic |
| ATGGCTAAGACAGCATTACC+CGG | 0.613591 | 8.3:-71363466 | None:intergenic |
| ACAGACAATGAACAATCAAG+TGG | 0.632140 | 8.3:+71363162 | None:intergenic |
| CGATGCAATCATCATTGGAG+TGG | 0.636920 | 8.3:-71363839 | None:intergenic |
| AGTGGTTGGATCAAATGAGA+GGG | 0.637995 | 8.3:+71363293 | MS.gene30313:CDS |
| CATACGAAAACAAGAATGAG+TGG | 0.644319 | 8.3:+71363275 | MS.gene30313:CDS |
| CAAATGGCTATAAATCCACC+CGG | 0.647825 | 8.3:+71363358 | MS.gene30313:CDS |
| ATCACTCCAGACAGGATCTG+GGG | 0.658221 | 8.3:-71363683 | None:intergenic |
| ATAGTATTCAGGGTATACAA+TGG | 0.671783 | 8.3:-71363485 | None:intergenic |
| ATAGGCCTAACGCCACCGGG+TGG | 0.692695 | 8.3:-71363373 | None:intergenic |
| ATGGCTATAAATCCACCCGG+TGG | 0.752819 | 8.3:+71363361 | MS.gene30313:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAATGATTTCCTCAAAAA+AGG | + | chr8.3:71363799-71363818 | MS.gene30313:CDS | 20.0% |
| !!! | TAACCATTAATATTGCTATT+CGG | - | chr8.3:71363663-71363682 | None:intergenic | 20.0% |
| ! | AATAATACAGCATGTTCTAA+AGG | + | chr8.3:71363421-71363440 | MS.gene30313:CDS | 25.0% |
| ! | CAAAAAAGGTGAATGTAAAA+AGG | + | chr8.3:71363813-71363832 | MS.gene30313:CDS | 25.0% |
| ! | TTACCGAATAGCAATATTAA+TGG | + | chr8.3:71363657-71363676 | MS.gene30313:CDS | 25.0% |
| ! | TTATCTCCATCATCTTTAAT+AGG | - | chr8.3:71363394-71363413 | None:intergenic | 25.0% |
| !!! | TTTTACATTCACCTTTTTTG+AGG | - | chr8.3:71363813-71363832 | None:intergenic | 25.0% |
| !!! | TTTTAGAACTAGTAGTGAAT+TGG | + | chr8.3:71363248-71363267 | MS.gene30313:CDS | 25.0% |
| AACAATCAAGTGGATAATCA+TGG | + | chr8.3:71363172-71363191 | MS.gene30313:CDS | 30.0% | |
| AACATATTGTGTTCCAGAAA+AGG | - | chr8.3:71363519-71363538 | None:intergenic | 30.0% | |
| ACACTGCGTATTATTATTTG+GGG | + | chr8.3:71363775-71363794 | MS.gene30313:CDS | 30.0% | |
| AGCAACAATAACGTTTCAAA+TGG | + | chr8.3:71363342-71363361 | MS.gene30313:CDS | 30.0% | |
| ATAGTATTCAGGGTATACAA+TGG | - | chr8.3:71363488-71363507 | None:intergenic | 30.0% | |
| CACACTGCGTATTATTATTT+GGG | + | chr8.3:71363774-71363793 | MS.gene30313:CDS | 30.0% | |
| TCACACTGCGTATTATTATT+TGG | + | chr8.3:71363773-71363792 | MS.gene30313:CDS | 30.0% | |
| TTTCAACATTGCTTCGTATT+TGG | + | chr8.3:71363719-71363738 | MS.gene30313:CDS | 30.0% | |
| !! | AGCATGTTCTAAAGGTTATA+AGG | + | chr8.3:71363429-71363448 | MS.gene30313:CDS | 30.0% |
| ACATTGCTTCGTATTTGGAT+TGG | + | chr8.3:71363724-71363743 | MS.gene30313:CDS | 35.0% | |
| AGGAAGTGAGAATAGTATTC+AGG | - | chr8.3:71363499-71363518 | None:intergenic | 35.0% | |
| CATACGAAAACAAGAATGAG+TGG | + | chr8.3:71363275-71363294 | MS.gene30313:CDS | 35.0% | |
| GGAAGTGAGAATAGTATTCA+GGG | - | chr8.3:71363498-71363517 | None:intergenic | 35.0% | |
| GTTAGGCCTATTAAAGATGA+TGG | + | chr8.3:71363385-71363404 | MS.gene30313:CDS | 35.0% | |
| TGAGTCATAGGTTTAGTATG+TGG | + | chr8.3:71363587-71363606 | MS.gene30313:CDS | 35.0% | |
| ! | ACTATTCTCACTTCCTTTTC+TGG | + | chr8.3:71363503-71363522 | MS.gene30313:CDS | 35.0% |
| ! | ATTGGATTGTTTTCCTTCTC+GGG | + | chr8.3:71363742-71363761 | MS.gene30313:CDS | 35.0% |
| ! | GATTGGATTGTTTTCCTTCT+CGG | + | chr8.3:71363741-71363760 | MS.gene30313:CDS | 35.0% |
| ! | GTTTTACTCTTGCTTATGAG+TGG | + | chr8.3:71363556-71363575 | MS.gene30313:CDS | 35.0% |
| !!! | AGTATGTGGCTTTTTTCGAT+AGG | + | chr8.3:71363601-71363620 | MS.gene30313:CDS | 35.0% |
| !!! | GTATGTGGCTTTTTTCGATA+GGG | + | chr8.3:71363602-71363621 | MS.gene30313:CDS | 35.0% |
| ACCAAAGAAAGGTTGGAGAA+AGG | + | chr8.3:71363225-71363244 | MS.gene30313:CDS | 40.0% | |
| ACCAATGACGAACCAAAGAA+AGG | + | chr8.3:71363214-71363233 | MS.gene30313:CDS | 40.0% | |
| ACCTTTCTCCAACCTTTCTT+TGG | - | chr8.3:71363229-71363248 | None:intergenic | 40.0% | |
| AGTGGTTGGATCAAATGAGA+GGG | + | chr8.3:71363293-71363312 | MS.gene30313:CDS | 40.0% | |
| ATGACGAACCAAAGAAAGGT+TGG | + | chr8.3:71363218-71363237 | MS.gene30313:CDS | 40.0% | |
| CAAATGGCTATAAATCCACC+CGG | + | chr8.3:71363358-71363377 | MS.gene30313:CDS | 40.0% | |
| CACTCCAATGATGATTGCAT+CGG | + | chr8.3:71363840-71363859 | MS.gene30313:CDS | 40.0% | |
| CGAAAACAAGAATGAGTGGT+TGG | + | chr8.3:71363279-71363298 | MS.gene30313:CDS | 40.0% | |
| GTGGAATTCGTTTGAGTCAT+AGG | + | chr8.3:71363575-71363594 | MS.gene30313:CDS | 40.0% | |
| TAAGGACTTGAAGCTATGTC+CGG | + | chr8.3:71363447-71363466 | MS.gene30313:CDS | 40.0% | |
| ! | ACCTTTCTTTGGTTCGTCAT+TGG | - | chr8.3:71363218-71363237 | None:intergenic | 40.0% |
| ! | TCTTTGGTTCGTCATTGGTT+GGG | - | chr8.3:71363213-71363232 | None:intergenic | 40.0% |
| ! | TTCTTTGGTTCGTCATTGGT+TGG | - | chr8.3:71363214-71363233 | None:intergenic | 40.0% |
| AAGGACTTGAAGCTATGTCC+GGG | + | chr8.3:71363448-71363467 | MS.gene30313:CDS | 45.0% | |
| ACCTCATTATCACTCCAGAC+AGG | - | chr8.3:71363694-71363713 | None:intergenic | 45.0% | |
| ATGGCTAAGACAGCATTACC+CGG | - | chr8.3:71363469-71363488 | None:intergenic | 45.0% | |
| CAAGCCGATGCAATCATCAT+TGG | - | chr8.3:71363847-71363866 | None:intergenic | 45.0% | |
| CGATGCAATCATCATTGGAG+TGG | - | chr8.3:71363842-71363861 | None:intergenic | 45.0% | |
| GAGTGGTTGGATCAAATGAG+AGG | + | chr8.3:71363292-71363311 | MS.gene30313:CDS | 45.0% | |
| TATCACTCCAGACAGGATCT+GGG | - | chr8.3:71363687-71363706 | None:intergenic | 45.0% | |
| TGTGAGTAACAAACCCGAGA+AGG | - | chr8.3:71363758-71363777 | None:intergenic | 45.0% | |
| TTATCACTCCAGACAGGATC+TGG | - | chr8.3:71363688-71363707 | None:intergenic | 45.0% | |
| TTTAATAGGCCTAACGCCAC+CGG | - | chr8.3:71363380-71363399 | None:intergenic | 45.0% | |
| ! | CTTTGGTTCGTCATTGGTTG+GGG | - | chr8.3:71363212-71363231 | None:intergenic | 45.0% |
| ! | TCCTGTCTGGAGTGATAATG+AGG | + | chr8.3:71363690-71363709 | MS.gene30313:CDS | 45.0% |
| ATCACTCCAGACAGGATCTG+GGG | - | chr8.3:71363686-71363705 | None:intergenic | 50.0% | |
| ATGGCTATAAATCCACCCGG+TGG | + | chr8.3:71363361-71363380 | MS.gene30313:CDS | 50.0% | |
| TTAATAGGCCTAACGCCACC+GGG | - | chr8.3:71363379-71363398 | None:intergenic | 50.0% | |
| TAAATCCACCCGGTGGCGTT+AGG | + | chr8.3:71363368-71363387 | MS.gene30313:CDS | 55.0% | |
| TGGTTACCCCAGATCCTGTC+TGG | + | chr8.3:71363677-71363696 | MS.gene30313:CDS | 55.0% | |
| ATAGGCCTAACGCCACCGGG+TGG | - | chr8.3:71363376-71363395 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 71363169 | 71363867 | 71363169 | ID=MS.gene30313 |
| chr8.3 | mRNA | 71363169 | 71363867 | 71363169 | ID=MS.gene30313.t1;Parent=MS.gene30313 |
| chr8.3 | exon | 71363169 | 71363867 | 71363169 | ID=MS.gene30313.t1.exon1;Parent=MS.gene30313.t1 |
| chr8.3 | CDS | 71363169 | 71363867 | 71363169 | ID=cds.MS.gene30313.t1;Parent=MS.gene30313.t1 |
| Gene Sequence |
| Protein sequence |
