AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene30403

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30403.t1 MTR_8g020970 98.598 927 13 0 1 927 1 927 0.0 1899
MS.gene30403.t1 MTR_8g020970 98.521 676 10 0 1 676 1 676 0.0 1379
MS.gene30403.t1 MTR_8g080230 54.276 842 377 6 89 926 78 915 0.0 944
MS.gene30403.t1 MTR_4g088810 54.448 843 350 10 90 927 82 895 0.0 919
MS.gene30403.t1 MTR_4g066170 47.753 890 428 13 44 926 46 905 0.0 844
MS.gene30403.t1 MTR_3g479460 48.212 811 403 8 122 926 103 902 0.0 810
MS.gene30403.t1 MTR_3g079450 48.212 811 403 10 122 926 102 901 0.0 805
MS.gene30403.t1 MTR_3g079420 45.972 844 438 10 90 926 32 864 0.0 766
MS.gene30403.t1 MTR_1g083020 45.848 855 452 9 79 926 7 857 0.0 757
MS.gene30403.t1 MTR_8g018420 47.122 834 424 11 100 926 32 855 0.0 754
MS.gene30403.t1 MTR_7g113410 46.753 847 436 13 91 926 24 866 0.0 750
MS.gene30403.t1 MTR_8g018730 47.445 822 411 11 113 926 55 863 0.0 749
MS.gene30403.t1 MTR_8g018735 47.381 821 411 12 114 926 57 864 0.0 748
MS.gene30403.t1 MTR_5g024020 46.780 823 416 13 113 926 57 866 0.0 741
MS.gene30403.t1 MTR_2g099560 47.023 823 412 15 113 926 55 862 0.0 738
MS.gene30403.t1 MTR_2g099570 46.036 845 434 14 91 926 29 860 0.0 736
MS.gene30403.t1 MTR_8g018730 47.253 819 387 12 113 926 55 833 0.0 730
MS.gene30403.t1 MTR_8g018570 46.594 822 420 13 114 926 58 869 0.0 725
MS.gene30403.t1 MTR_8g018520 45.676 821 429 10 113 926 57 867 0.0 722
MS.gene30403.t1 MTR_1g104650 44.574 857 458 12 84 926 2 855 0.0 721
MS.gene30403.t1 MTR_8g020990 45.432 821 431 11 113 926 57 867 0.0 717
MS.gene30403.t1 MTR_8g018690 45.477 818 430 9 113 926 59 864 0.0 714
MS.gene30403.t1 MTR_8g018510 45.455 814 427 11 120 926 65 868 0.0 711
MS.gene30403.t1 MTR_3g081130 46.610 826 412 12 113 926 49 857 0.0 709
MS.gene30403.t1 MTR_8g018430 44.580 821 438 11 113 926 50 860 0.0 705
MS.gene30403.t1 MTR_3g081130 47.876 777 387 10 156 926 21 785 0.0 704
MS.gene30403.t1 MTR_8g018550 46.163 821 425 11 113 926 57 867 0.0 702
MS.gene30403.t1 MTR_5g024020 44.836 823 396 15 113 926 57 830 0.0 689
MS.gene30403.t1 MTR_8g018690 44.377 818 419 10 113 926 59 844 0.0 685
MS.gene30403.t1 MTR_8g018450 43.688 808 408 10 128 926 58 827 0.0 673
MS.gene30403.t1 MTR_8g018590 44.052 765 380 12 177 926 91 822 0.0 634
MS.gene30403.t1 MTR_8g018650 43.286 767 381 9 164 926 90 806 0.0 612
MS.gene30403.t1 MTR_8g018620 43.229 768 380 11 164 926 90 806 0.0 606
MS.gene30403.t1 MTR_8g011340 38.582 832 487 11 65 882 1 822 0.0 576
MS.gene30403.t1 MTR_8g018730 48.727 589 287 8 344 926 4 583 0.0 563
MS.gene30403.t1 MTR_3g079450 46.774 558 285 7 122 676 102 650 9.47e-177 527
MS.gene30403.t1 MTR_8g018735 45.709 571 290 11 114 676 57 615 2.84e-156 474
MS.gene30403.t1 MTR_6g452500 37.908 612 358 9 313 913 11 611 2.83e-133 414
MS.gene30403.t1 MTR_8g021020 46.835 395 207 3 534 926 37 430 2.85e-122 378
MS.gene30403.t1 MTR_7g012030 58.824 119 49 0 578 696 85 203 1.01e-43 158
MS.gene30403.t1 MTR_8g463810 36.000 225 80 2 488 712 197 357 1.16e-39 152
MS.gene30403.t1 MTR_6g046330 53.077 130 50 1 578 696 75 204 3.73e-38 145
MS.gene30403.t1 MTR_4g088805 58.696 92 38 0 836 927 1 92 1.11e-28 110
MS.gene30403.t1 MTR_0248s0010 67.442 43 2 1 732 774 3 33 5.97e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30403.t1 AT1G17420 69.903 927 269 5 1 926 1 918 0.0 1370
MS.gene30403.t1 AT1G72520 70.695 935 255 10 1 926 1 925 0.0 1354
MS.gene30403.t1 AT1G67560 51.348 927 423 14 7 926 11 916 0.0 904
MS.gene30403.t1 AT3G45140 44.470 868 452 14 66 926 51 895 0.0 755
MS.gene30403.t1 AT1G55020 45.745 846 443 13 90 926 20 858 0.0 745
MS.gene30403.t1 AT3G22400 44.548 862 457 11 83 926 27 885 0.0 731
MS.gene30403.t1 AT3G45140 47.500 760 383 10 173 926 4 753 0.0 728

Find 187 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTGAAAATGTGTTCATTT+TGG 0.118061 8.1:-78429163 None:intergenic
TTTACGGTTGACTCAAATTT+TGG 0.128121 8.1:+78426877 MS.gene30403:CDS
GCAAGATCAATTCCTTTATC+TGG 0.137436 8.1:-78428175 None:intergenic
CTTTAGATGCAACTACTAAT+TGG 0.156046 8.1:+78431354 MS.gene30403:CDS
GAGCAGAAAGACTTGATTTA+AGG 0.159246 8.1:-78429224 None:intergenic
ACTCTCTGTCATTGATTATT+AGG 0.161431 8.1:-78433018 None:intergenic
TTTGGTCAGCAATAGAAAAT+TGG 0.164309 8.1:+78432957 MS.gene30403:CDS
TATCAATTCTCTGCGGAAAT+TGG 0.203663 8.1:+78433457 MS.gene30403:CDS
TTAATTATTAGATACCTTAT+TGG 0.232273 8.1:-78427055 None:intergenic
CAGAAGATGGTCTTCTAATT+TGG 0.251107 8.1:+78432939 MS.gene30403:CDS
TCTTGTGGACCATAGATTTC+AGG 0.265813 8.1:-78430420 None:intergenic
AAGTCCTAACAATCTTAATT+TGG 0.267658 8.1:+78433137 MS.gene30403:CDS
CATTCCAAATTAAGATTGTT+AGG 0.277408 8.1:-78433141 None:intergenic
TACCCTGGCTTGTGGATTCT+TGG 0.281500 8.1:-78429371 None:intergenic
TCGTCTGACGAAGAGTATTT+AGG 0.285117 8.1:+78433379 MS.gene30403:CDS
GAATTACTTGCTCCTAGTTC+TGG 0.295134 8.1:+78433559 MS.gene30403:CDS
TTGATTATTAGGCTTGGATT+TGG 0.309327 8.1:-78433007 None:intergenic
TCCTCAAATCTTTCATCTCT+TGG 0.311154 8.1:-78429138 None:intergenic
TCAGGATCTAATTTACTAAC+AGG 0.316657 8.1:-78430402 None:intergenic
GTTGCAAGCTACAAAATATA+TGG 0.317917 8.1:+78433330 MS.gene30403:CDS
TTGTCACTGTCGTTCAATTT+CGG 0.321906 8.1:-78433106 None:intergenic
TAATTACACAGCTGAATTTA+CGG 0.322203 8.1:+78426861 MS.gene30403:CDS
CCACTCACTTGATATAATCT+TGG 0.323901 8.1:-78429412 None:intergenic
AATGAATTCTCAGATGTTGA+TGG 0.326863 8.1:+78429261 MS.gene30403:CDS
GTCAAAGAAGACTAATATTA+AGG 0.327000 8.1:+78426828 MS.gene30403:CDS
ATCCTTCAACACTGCTTCAT+TGG 0.329544 8.1:-78426803 None:intergenic
GATATGAAACTTGTATATTC+AGG 0.338356 8.1:-78433268 None:intergenic
GTATTAATCTTCTCATCAAC+GGG 0.342100 8.1:-78433231 None:intergenic
GAAAAGAAAATTCTCTTTCC+AGG 0.342282 8.1:-78427033 None:intergenic
ATGGAAACAAAAGAGTTGTT+AGG 0.343668 8.1:+78426327 MS.gene30403:CDS
AATTTCGGACAGTATCCTTA+TGG 0.355580 8.1:+78433187 MS.gene30403:CDS
CATTTATATTGTCTGCTCAT+AGG 0.356521 8.1:+78431854 MS.gene30403:CDS
CTGTCATTGATTATTAGGCT+TGG 0.359024 8.1:-78433013 None:intergenic
GGATCCAATAACTTGAAAAT+TGG 0.359274 8.1:-78431895 None:intergenic
CAACATGCTGCACTTAATTT+CGG 0.362410 8.1:+78433172 MS.gene30403:CDS
CTATGAGCAGACAATATAAA+TGG 0.371741 8.1:-78431853 None:intergenic
GTAAGTCTCTCAATGGTAAC+AGG 0.380176 8.1:-78430369 None:intergenic
TTAGAAAATATAACAATTGA+AGG 0.381425 8.1:+78426949 MS.gene30403:CDS
AGAGGCAACAACCTTCGATT+TGG 0.381540 8.1:+78433404 MS.gene30403:CDS
GTGCTTCATAACTTGCTTCC+TGG 0.384287 8.1:+78429201 MS.gene30403:CDS
GATCTTGGAAACCCAGATAA+AGG 0.388829 8.1:+78428163 MS.gene30403:CDS
GGCCCTAACAAAAGAATTCA+TGG 0.391351 8.1:+78426208 MS.gene30403:CDS
CCATGGTTCGTCTTTCATAT+CGG 0.393755 8.1:-78433080 None:intergenic
AGAATTCATGGGTTCTTCAT+TGG 0.395604 8.1:+78426220 MS.gene30403:CDS
GGTGATGAAGAAGGAAATAT+TGG 0.401225 8.1:-78426251 None:intergenic
TTGAGGAACCGTTGCGGTGC+TGG 0.401483 8.1:+78433523 MS.gene30403:CDS
TTGGTCAGCAATAGAAAATT+GGG 0.402009 8.1:+78432958 MS.gene30403:CDS
GAACTTAGCCTGCCTCCATC+TGG 0.403989 8.1:+78431296 MS.gene30403:CDS
TATGACACATACAATGATCT+TGG 0.412730 8.1:+78428148 MS.gene30403:CDS
CACTCACTTGATATAATCTT+GGG 0.413543 8.1:-78429411 None:intergenic
AGAAGACCATCTTCTGCATA+AGG 0.416449 8.1:-78432932 None:intergenic
TCTTGGGAATGTCATACTTA+AGG 0.423843 8.1:-78429395 None:intergenic
AAATTAAGATTGTTAGGACT+TGG 0.428810 8.1:-78433135 None:intergenic
TTTAGATGCAACTACTAATT+GGG 0.429267 8.1:+78431355 MS.gene30403:CDS
TGAGTAGTCCAACACTATAA+AGG 0.430450 8.1:-78429284 None:intergenic
TTAGACCATGTGGTTGTGTT+GGG 0.432526 8.1:-78432889 None:intergenic
GATCTTGAGGGAGGCTATCC+TGG 0.433367 8.1:-78432062 None:intergenic
TGATTATTAGGCTTGGATTT+GGG 0.433593 8.1:-78433006 None:intergenic
TGTTGGACTACTCATTAAGT+TGG 0.436827 8.1:+78429293 MS.gene30403:CDS
AGGCTAAGTTCAATTGCAAC+AGG 0.441630 8.1:-78431284 None:intergenic
TCTTTAAGTGCAGACTCTTG+TGG 0.444467 8.1:-78430435 None:intergenic
CAAGATCAATTCCTTTATCT+GGG 0.447942 8.1:-78428174 None:intergenic
ATTGAGTCTTGCTTCACTCC+TGG 0.448673 8.1:+78431985 MS.gene30403:CDS
GACCAATGAAGCAGTGTTGA+AGG 0.454038 8.1:+78426801 MS.gene30403:CDS
CAAGCTAGAAAAGATCTTCC+TGG 0.457008 8.1:+78427015 MS.gene30403:CDS
TTTGTAGCTTGCAACAAACT+AGG 0.458682 8.1:-78433322 None:intergenic
TTGGTGCACACCAGCATCAT+TGG 0.460866 8.1:-78431408 None:intergenic
TTGAGAAGGTGATTGATAGT+AGG 0.462008 8.1:+78433485 MS.gene30403:CDS
GCTCTAACCTTAAATTTCAC+TGG 0.462917 8.1:-78426461 None:intergenic
GAAAGACTTGATTTAAGGCC+AGG 0.464489 8.1:-78429219 None:intergenic
ACAATTGAAGGATTTGCTAC+TGG 0.466343 8.1:+78426961 MS.gene30403:CDS
AAATTAGATCCTGAAATCTA+TGG 0.468171 8.1:+78430411 MS.gene30403:CDS
GTGGCCTACATTGATTGATT+CGG 0.469250 8.1:-78433056 None:intergenic
AACTCCAGCTATTGCTTGAC+GGG 0.470233 8.1:-78430343 None:intergenic
TCTTCACTGATAGCTGCAAC+AGG 0.474972 8.1:-78426371 None:intergenic
GTTGGACTACTCATTAAGTT+GGG 0.477312 8.1:+78429294 MS.gene30403:CDS
ACCAAGAGATGAAAGATTTG+AGG 0.477798 8.1:+78429137 MS.gene30403:CDS
TTTAGACCATGTGGTTGTGT+TGG 0.478176 8.1:-78432890 None:intergenic
TTTGGAAAGGATCAATGCCT+TGG 0.478720 8.1:+78431199 MS.gene30403:CDS
GATCTTGCTAGACCAACTCT+TGG 0.479530 8.1:+78428190 MS.gene30403:CDS
ATATGAAACTTGTATATTCA+GGG 0.484202 8.1:-78433267 None:intergenic
CCATTCTTCTGTTGAAAACA+TGG 0.484479 8.1:-78426272 None:intergenic
GAAGATGATGATATAGAAGA+AGG 0.486786 8.1:-78426416 None:intergenic
CCATGTGGTTGTGTTGGGTC+TGG 0.492360 8.1:-78432884 None:intergenic
TCATCTCTTGGTATATACAT+AGG 0.492403 8.1:-78429126 None:intergenic
TAACTCCAGCTATTGCTTGA+CGG 0.493184 8.1:-78430344 None:intergenic
CGGCCAGTACGGCACCGTCT+AGG 0.495310 8.1:-78428235 None:intergenic
ATGAAGCAGTGTTGAAGGAT+TGG 0.497542 8.1:+78426806 MS.gene30403:CDS
TACCCTAGACGGTGCCGTAC+TGG 0.498027 8.1:+78428232 MS.gene30403:CDS
GAAGACCATCTTCTGCATAA+GGG 0.501628 8.1:-78432931 None:intergenic
TGCACCAACTTGCCCACCAT+TGG 0.505334 8.1:+78431423 MS.gene30403:CDS
CTCAAAAGTTTAATCCCAGC+AGG 0.506743 8.1:-78428055 None:intergenic
TTTATCACAGTAATTGCTCC+TGG 0.507282 8.1:-78426904 None:intergenic
CATGTTTGCTCCAATGATGC+TGG 0.508665 8.1:+78431398 MS.gene30403:CDS
GGCCGTGAACCATCTGATAC+AGG 0.510574 8.1:+78428253 MS.gene30403:CDS
TACCTGTATCAGATGGTTCA+CGG 0.511896 8.1:-78428255 None:intergenic
CTGCTGCTTACAAAACCAAC+TGG 0.511913 8.1:+78432031 MS.gene30403:CDS
GATCCAAGAATCCACAAGCC+AGG 0.513956 8.1:+78429368 MS.gene30403:CDS
CTTGCTCCTAGTTCTGGACC+TGG 0.515196 8.1:+78433565 MS.gene30403:CDS
GTACGGCACCGTCTAGGGTA+TGG 0.515752 8.1:-78428229 None:intergenic
CTACCCGATGATACACCTGC+TGG 0.515885 8.1:+78428040 MS.gene30403:CDS
GGTGTATCATCGGGTAGATA+AGG 0.517991 8.1:-78428034 None:intergenic
GTTGATGGCCTTTATAGTGT+TGG 0.523396 8.1:+78429276 MS.gene30403:CDS
CTTAAGGATACCCTGGCTTG+TGG 0.525590 8.1:-78429379 None:intergenic
ATCTCCATGGCATAGCGACC+AGG 0.526081 8.1:-78432003 None:intergenic
GACCTGGTGTTACATGCAGA+GGG 0.527475 8.1:+78433581 MS.gene30403:CDS
GGTATTAATCTTCTCATCAA+CGG 0.530886 8.1:-78433232 None:intergenic
TTTGCCCGTCAAGCAATAGC+TGG 0.532353 8.1:+78430339 MS.gene30403:CDS
CTTACCAATGGTGGGCAAGT+TGG 0.533372 8.1:-78431427 None:intergenic
ATCCCAGCAGGTGTATCATC+GGG 0.533446 8.1:-78428043 None:intergenic
CACTCCTGGTCGCTATGCCA+TGG 0.533642 8.1:+78431999 MS.gene30403:CDS
TGTTTGGTCCAGATGGAGGC+AGG 0.533655 8.1:-78431304 None:intergenic
ATTTCAGATCCTCCAAGAGT+TGG 0.535524 8.1:-78428202 None:intergenic
CTTGCTAGACCAACTCTTGG+AGG 0.537115 8.1:+78428193 MS.gene30403:CDS
CAACAACCTTCGATTTGGAC+AGG 0.537155 8.1:+78433409 MS.gene30403:CDS
TAATTATTACCTGTATCAGA+TGG 0.538174 8.1:-78428262 None:intergenic
GATTACCCTTATGCAGAAGA+TGG 0.539224 8.1:+78432926 MS.gene30403:CDS
GAAAGGATCAATGCCTTGGA+TGG 0.543205 8.1:+78431203 MS.gene30403:CDS
GTAACACCAGGTCCAGAACT+AGG 0.543782 8.1:-78433571 None:intergenic
TTGCTAACAATGTGAGGCAA+TGG 0.546196 8.1:-78429345 None:intergenic
CTTGGTATATACATAGGAAG+TGG 0.550676 8.1:-78429120 None:intergenic
TCCACACATGAGGTACACTT+TGG 0.551392 8.1:+78431915 MS.gene30403:CDS
GGACCTGGTGTTACATGCAG+AGG 0.558911 8.1:+78433580 MS.gene30403:CDS
AATCCCAGCAGGTGTATCAT+CGG 0.559593 8.1:-78428044 None:intergenic
ACCTGCGGATGAGATCTTGA+GGG 0.562889 8.1:-78432074 None:intergenic
GGCCAGTACGGCACCGTCTA+GGG 0.564885 8.1:-78428234 None:intergenic
GATCGAGTGTTTGGTCCAGA+TGG 0.566962 8.1:-78431311 None:intergenic
TTAGTAGTTGCATCTAAAGC+AGG 0.568173 8.1:-78431350 None:intergenic
TTTGGCACGTAACCTCCATA+AGG 0.568931 8.1:-78433202 None:intergenic
TGAAAACATGGTGATGAAGA+AGG 0.569969 8.1:-78426260 None:intergenic
GTTGATGAGAAGATTAATAC+CGG 0.571822 8.1:+78433234 MS.gene30403:CDS
AAACTGAGAGGTGATGGAAA+AGG 0.571903 8.1:+78428094 MS.gene30403:CDS
AAGGTGATTGATAGTAGGAA+TGG 0.572871 8.1:+78433490 MS.gene30403:CDS
TCCCTCAAGATCTCATCCGC+AGG 0.574537 8.1:+78432073 MS.gene30403:CDS
GGATCTAATTTACTAACAGG+TGG 0.574715 8.1:-78430399 None:intergenic
GTGCAGAGGATAAATTTGCT+TGG 0.574838 8.1:+78430301 MS.gene30403:intron
GATCTAATTTACTAACAGGT+GGG 0.574850 8.1:-78430398 None:intergenic
GCTACGCACACATGCTTGCA+TGG 0.575061 8.1:+78431828 MS.gene30403:CDS
GGAATGGCGATAGGAATTTG+AGG 0.578036 8.1:+78433506 MS.gene30403:CDS
GAAAACTGTAAGTCTCTCAA+TGG 0.578316 8.1:-78430376 None:intergenic
ATCCTGGTCAAAACGCCAGT+TGG 0.579614 8.1:-78432046 None:intergenic
AAATGTATCCATACCCTAGA+CGG 0.586694 8.1:+78428221 MS.gene30403:CDS
ATCCAAGAATCCACAAGCCA+GGG 0.589581 8.1:+78429369 MS.gene30403:CDS
TTCGGACAGTATCCTTATGG+AGG 0.590508 8.1:+78433190 MS.gene30403:CDS
ATTATTAGCTTTAGACCATG+TGG 0.590586 8.1:-78432899 None:intergenic
TACCCGATGATACACCTGCT+GGG 0.591254 8.1:+78428041 MS.gene30403:CDS
TACCTGCGGATGAGATCTTG+AGG 0.594715 8.1:-78432075 None:intergenic
CAGATGGTTCACGGCCAGTA+CGG 0.595137 8.1:-78428246 None:intergenic
AGCAGCAGAACTAATCTCCA+TGG 0.597060 8.1:-78432016 None:intergenic
TGGATCTTGCTAACAATGTG+AGG 0.598996 8.1:-78429351 None:intergenic
GTATGCTTGCTTACCAATGG+TGG 0.599350 8.1:-78431436 None:intergenic
GCATCACCTGTCCAAATCGA+AGG 0.600030 8.1:-78433415 None:intergenic
CAGAGCTTGATCAATGCAGA+TGG 0.602184 8.1:+78431958 MS.gene30403:CDS
TCGTTCAATTTCGGCCACCA+TGG 0.607867 8.1:-78433097 None:intergenic
TGTCATACTTAAGGATACCC+TGG 0.608790 8.1:-78429386 None:intergenic
AGGAATTTGAGGAACCGTTG+CGG 0.608855 8.1:+78433517 MS.gene30403:CDS
TCCAAAGTGTACCTCATGTG+TGG 0.612282 8.1:-78431916 None:intergenic
CTAGGAGCAAGTAATTCATA+CGG 0.613547 8.1:-78433553 None:intergenic
CGAGTGTTTGGTCCAGATGG+AGG 0.614080 8.1:-78431308 None:intergenic
GCCCTAACAAAAGAATTCAT+GGG 0.614296 8.1:+78426209 MS.gene30403:CDS
TTTAAATGGTACCTCTTGGA+CGG 0.616838 8.1:-78430490 None:intergenic
ACCCCTCTGCATGTAACACC+AGG 0.617716 8.1:-78433583 None:intergenic
ATTAAGTGCAGCATGTTGCG+CGG 0.618193 8.1:-78433167 None:intergenic
ATGCAACTACTAATTGGGTG+TGG 0.623131 8.1:+78431360 MS.gene30403:CDS
ACGAAGAGTATTTAGGTGAG+AGG 0.628094 8.1:+78433386 MS.gene30403:CDS
ATGACAGAGAGTTACAAGCT+TGG 0.632533 8.1:+78433029 MS.gene30403:CDS
TACTCCGAATCAATCAATGT+AGG 0.641645 8.1:+78433052 MS.gene30403:CDS
GCAAAACACCAGCACCGCAA+CGG 0.641663 8.1:-78433531 None:intergenic
TTGATAGTAGGAATGGCGAT+AGG 0.646766 8.1:+78433497 MS.gene30403:CDS
GCTTGAACCCAAGAATTGCA+AGG 0.647001 8.1:-78426997 None:intergenic
GGAAATTGGAAAGATTGAGA+AGG 0.652309 8.1:+78433471 MS.gene30403:CDS
TATGCTTGCTTACCAATGGT+GGG 0.656904 8.1:-78431435 None:intergenic
TATTACTTGTGAGAGTCGTG+TGG 0.661476 8.1:+78429092 MS.gene30403:CDS
CCGATATGAAAGACGAACCA+TGG 0.663394 8.1:+78433080 MS.gene30403:CDS
TAATGGCATGACCGTCCAAG+AGG 0.670114 8.1:+78430479 MS.gene30403:CDS
TGCGGATGAGATCTTGAGGG+AGG 0.673318 8.1:-78432071 None:intergenic
AGTGAGGAACAAGATCAAAG+AGG 0.677524 8.1:+78426493 MS.gene30403:CDS
AGTTATTGGATCCACACATG+AGG 0.685129 8.1:+78431905 MS.gene30403:CDS
GTTCGTCTTTCATATCGGCG+TGG 0.688334 8.1:-78433075 None:intergenic
CCAGACCCAACACAACCACA+TGG 0.693358 8.1:+78432884 MS.gene30403:CDS
ACCTGGTGTTACATGCAGAG+GGG 0.702111 8.1:+78433582 MS.gene30403:CDS
AGTAATAGTTCACATAAGTG+CGG 0.704279 8.1:-78432982 None:intergenic
ATTAATCTTCTCATCAACGG+GGG 0.724917 8.1:-78433229 None:intergenic
ATATGAAAGACGAACCATGG+TGG 0.746586 8.1:+78433083 MS.gene30403:CDS
TATTAATCTTCTCATCAACG+GGG 0.754646 8.1:-78433230 None:intergenic
ACAGGCTTTAGAGTACCAAG+TGG 0.757581 8.1:-78431266 None:intergenic
TTAGAGCTGTTGTAACAGTG+AGG 0.760433 8.1:+78426477 MS.gene30403:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr8.1 gene 78426206 78433627 78426206 ID=MS.gene30403
chr8.1 mRNA 78426206 78433627 78426206 ID=MS.gene30403.t1;Parent=MS.gene30403
chr8.1 exon 78426206 78426611 78426206 ID=MS.gene30403.t1.exon1;Parent=MS.gene30403.t1
chr8.1 CDS 78426206 78426611 78426206 ID=cds.MS.gene30403.t1;Parent=MS.gene30403.t1
chr8.1 exon 78426782 78427062 78426782 ID=MS.gene30403.t1.exon2;Parent=MS.gene30403.t1
chr8.1 CDS 78426782 78427062 78426782 ID=cds.MS.gene30403.t1;Parent=MS.gene30403.t1
chr8.1 exon 78428034 78428274 78428034 ID=MS.gene30403.t1.exon3;Parent=MS.gene30403.t1
chr8.1 CDS 78428034 78428274 78428034 ID=cds.MS.gene30403.t1;Parent=MS.gene30403.t1
chr8.1 exon 78429091 78429426 78429091 ID=MS.gene30403.t1.exon4;Parent=MS.gene30403.t1
chr8.1 CDS 78429091 78429426 78429091 ID=cds.MS.gene30403.t1;Parent=MS.gene30403.t1
chr8.1 exon 78430307 78430500 78430307 ID=MS.gene30403.t1.exon5;Parent=MS.gene30403.t1
chr8.1 CDS 78430307 78430500 78430307 ID=cds.MS.gene30403.t1;Parent=MS.gene30403.t1
chr8.1 exon 78431143 78431444 78431143 ID=MS.gene30403.t1.exon6;Parent=MS.gene30403.t1
chr8.1 CDS 78431143 78431444 78431143 ID=cds.MS.gene30403.t1;Parent=MS.gene30403.t1
chr8.1 exon 78431828 78432094 78431828 ID=MS.gene30403.t1.exon7;Parent=MS.gene30403.t1
chr8.1 CDS 78431828 78432094 78431828 ID=cds.MS.gene30403.t1;Parent=MS.gene30403.t1
chr8.1 exon 78432871 78433627 78432871 ID=MS.gene30403.t1.exon8;Parent=MS.gene30403.t1
chr8.1 CDS 78432871 78433627 78432871 ID=cds.MS.gene30403.t1;Parent=MS.gene30403.t1
Gene Sequence

>MS.gene30403

ATGGCCCTAACAAAAGAATTCATGGGTTCTTCATTGGTAGAGAGACCAATATTTCCTTCTTCATCACCATGTTTTCAACAGAAGAATGGTGGATTCTTGATCAGTCCTGTTTTTGTTCCTTATGGAAACAAAAGAGTTGTTAGGTTGAGAAAATCTGCAAAGTTTCCTGTTGCAGCTATCAGTGAAGATTTGATGAAAAACTCTTCTTCACCTTCTTCTATATCATCATCTTCTTCTTCTGTTCCTGCTGAAAAACCAGTGAAATTTAAGGTTAGAGCTGTTGTAACAGTGAGGAACAAGATCAAAGAGGATTTCAAAGAGACTTTTGTGAAACATCTTGATGCTTTTACTGATAGGATTGGAAGAAATGTTGTTCTTGAACTTTTTAGCACTGAGATTGATCCAAGTAAGACTTTTCTTTAGTGTGTTAATTAAAAATCAATATTTACTTTGTACACTTAATTTTAATTTCCTTGATTTTGATAATTTTTAGTTCTTTATGAAATTTTATTTGGATTAGCTTTAAATTTATAACTGAATTAAATTATTGGTGATTTTTATGTTGTGATCTTTCAGAAACAAAAGCTGCAAAGAAGACCAATGAAGCAGTGTTGAAGGATTGGTCAAAGAAGACTAATATTAAGGCAGAGAGAGTTAATTACACAGCTGAATTTACGGTTGACTCAAATTTTGGAGAACCAGGAGCAATTACTGTGATAAATAATCATCAACAAGAATTTTACTTAGAAAATATAACAATTGAAGGATTTGCTACTGGAGCATTTCATTTTCCTTGCAATTCTTGGGTTCAAGCTAGAAAAGATCTTCCTGGAAAGAGAATTTTCTTTTCCAATAAGGTATCTAATAATTAATTCTTCAACTCTTTTTTTATTTTTTTTGTTATTTATCTGTCATCATAACATGGCCCAGTAAGGAAAAAAAAAGTAGTACAGCCAAAAAAGTAGTATCATAAGGCAACAATATTTGTTTATTCATTTTTTCAACGTGAGAAATAAGGTGAAAATTGAAAGTTAAAAGGTATAATAATTTTTGTTTTGAAGAATCTTGAAAAGGCACAATAATTCCAAAAGTTCATTTTACCGTAAAAATATTGAATGTTATTTAATAATTATTTTATGTGCCAAAACCAAGCAGTCCGTGATTTTATAATTATTGTTATATATCATTGCATTTTTTAATTAATTATCCACGATAAAGTGATATACTAATAAACAATTAACCTAGAAAATCATATAGTCAAATTTATGCATATGGACCAAGCACCATTGAATTGACCTGACGACTACCTACGGTATGAATTGGGCAAAGACACCGTTGAATTGAATACTAGTAGTACTTGTCAAGAAAAAAAATAATACTAGCCAATACCCTCTTTATCTTTATTTTTCGAACATTCTCTAACACTCACTCGTTTATTGGATTAAATTAAGGTGAGTCTCACACGTTGAAAATTGATTTTCTTAAAGTGATGAAACATATATTGATTTTATCTAATAAGAGAGGTGAGTATTAGAAACTATTAAAACAAAAGTATCTCTCGCATTTCTCTTATTTTTTTGACTAGTACTAGTAGTCTTTATGGTCGAGGATGAATGTGGAACAATAATCTTACCCTCGGCATTATTCAGCATTTACCTAATGTAAATTTATACAAATAATAAAGTGGTAACATTCCCTAAATAAAAAAATAAAGTGGTAACATTTACCATTCTCGATCTTTTGACTAAGATCAAGTGTAGTATAAATATTCTTTTCGAAATAAAATGGTAACATATGTTTGATTTAATGTGCGATTTACTTTGAACAGCCTTATCTACCCGATGATACACCTGCTGGGATTAAACTTTTGAGAGAGAAAGACCTGAAAAAACTGAGAGGTGATGGAAAAGGAGTTAGAAAATTATCTGACAGAATATATGATTATGACACATACAATGATCTTGGAAACCCAGATAAAGGAATTGATCTTGCTAGACCAACTCTTGGAGGATCTGAAATGTATCCATACCCTAGACGGTGCCGTACTGGCCGTGAACCATCTGATACAGGTAATAATTAATTATATAAGCAATTTTACACTACTAATGATTAATGAATATTGCATTGTATCCTTTAGTAGGGTGTTAAACTATATTTCTATCCTTTAAAGTGCGATGTTAGATGATTCGGGTTCGAACTTCGACTCATTCATTTGTATGTATAAGTAAATTTCCAACGACTATTGTGATTTTATCTATCTAAAAAAAAGTCTGTTGTTAGAATACTATATGAAACTAAGAAGTTGACAAAAACATAATTTCTCTTTATGAAATTATAAGTTGTCCTCATTTTAGTTGCACATTGTATTCTAGGTTGTATTTAAAGTTATATCTTGTGGTCTTGTAAATGCAATTCAGATGATAAAATTCTAAAATGTTACAATGACATTTGATATGATGGTGTGTGGGCTTAAACTCGCATTTCTCCACTTCTCTATACTTAGTGTGTATCTATGTACGTGTGAATTTCGCTGCAAATCTCCCTTGGAAAAAAAAGTGTGATAGCTTGGAAATATAAAAAATTATTTTACATTCAAATTTAGTAAATTTTTACAAATCAATTTGATTTTAAGGATTAAAATTTATACTAATTTGATGTATTTTCTAACATAGGATCTTCTCTGGACCAACTTGTGGGCACCGACATCTTGTGATGCTTTACAGTGGTGTCATGGCATGCTATAATATTATTTTCTTAAATATTTGTACAAATATTTACTTGATAAAATATGATTATCTGTTTAAAATTATGTAGATTGTAATATGTTACTATGCTTTGTGGTGATTCTAAATAACTAAACAAAATTGAATTGGATGGTGCTGCAGATATTACTTGTGAGAGTCGTGTGGAGAAGCCACTTCCTATGTATATACCAAGAGATGAAAGATTTGAGGAATCCAAAATGAACACATTTTCAGTGAAGAGGCTAAAAGGAGTGCTTCATAACTTGCTTCCTGGCCTTAAATCAAGTCTTTCTGCTCAAAACAGAGATTTCAATGAATTCTCAGATGTTGATGGCCTTTATAGTGTTGGACTACTCATTAAGTTGGGTTTGCAAGATGATATTTTGAAGAAACTTCCATTGCCTCACATTGTTAGCAAGATCCAAGAATCCACAAGCCAGGGTATCCTTAAGTATGACATTCCCAAGATTATATCAAGTGAGTGGAATATGCACTTTTTTTTTTTTTTGAGGGAATATGCACTTGTTATTGTCGATTTCTTGTCTTCCTTGCTTAAAAAATTGGCTACATAATTTTTCATAAGTAAATTTTTGGTTTAAATACACTTTTAGTCTTCTCGTCCTAACAATTTTGATTCTACATTTTTTAAATTTTTTTAATATTTCCGCATTACTAAATTTTGGGCTTAATTTTACTAGTGCGTTAGTTTGAATTACACTTTTTGTCATTGGATGGAAGATGTAGCATCGTATATTTTTTGAGATATAATTTTCTTAGGGTTTTACACTTTCTTCATTTAGAAAACTTCCTCTTTCTTTCTATAAAATTTCTAAACTTTATATTTCAAAATTGAATTATGTCTTCATAACAAATTTAACTTAGATTTGAAATATCTCCAAATCATATACCAATTACACCAAAGGAAAATTTATATTTTTTTACTTTCCCTTTCATTTGCAATGTGGTGGGGTCTTTGGCCATGAATATTCTATAATGGCATACAAGCATGCCATATGAAAAGATAGTGCTACAAATTAAAGAGTGCCACACTACAACATGTTGTTTGGCTTTTAGTGTTTGTACAATTTCAACCCAAAAAGATGAGTTTTCGGTTCAAAGCTAGTAGATCTAATGTTTATACCATTCCTTTTATATATAAAAAAATTACAACTGGGCCAATTTGTTATTAATTATTACTTGTTCATTTGAAACTAAACTGTCACCAAAGCTGTACTAATCAATTGTTAATTTTAAATGTTTCACATGTGTGTCTAGTTATTATAAACTGTTGATATAAAAATTAAAGTTCTTGTTTAATTAATTGTGAAATTATTGATCTCATGTGCCTAATGTGCAGAGGATAAATTTGCTTGGTTGAGAGATGATGAATTTGCCCGTCAAGCAATAGCTGGAGTTAATCCTGTTACCATTGAGAGACTTACAGTTTTCCCACCTGTTAGTAAATTAGATCCTGAAATCTATGGTCCACAAGAGTCTGCACTTAAAGAAGAGCACATTTTAAATCAACTTAATGGCATGACCGTCCAAGAGGTACCATTTAAAGCATTCAAAAACTTTTTCCTTTTTGGTACAATGCATTCATAAACTTACTAACCATGTTACCTAATCAAGTGCCTTGTGAATTGTTTTTTCAAACTATTTAACCAATTATAACATTTCCCATGTACCTTAAAAATAAACATATTTCTCATGGATTAATTTTTTCACTTGACATCAAATTTATAATGGTCACCAATACGTGGTTTGGTTCAAGTAGTGAGTGATTTGGATACTTTAAACAAATGGTCAGAGATTTGACTACTCAGGATCGACCTAAAGACTAAGCAATTTAAGTTAGGGTTTTAAGTCTCACTTCAAAATTTGTTTTAGACTTCTAAACATATTGTGTACGTCCTGAGGCAACTAACTCTTGTATATGGAGAAAAACCAGTTAAAAATGGCGAATCTATCATGGTGCCCAGTAGGTTCCAACAAAAATTACCACTGTTAAAGGACAATGATACTTCGTGCCTATAACTTGATTTTAAAGAAATAAATTTAAAATTATCCTATGTTTGCTCTTGAATGCACAATTCAATACTAGTAGATCATATAAATTTTGACATTTTTTTTTATTAATTTACTTATTTGCACTAAACTCCCACTTTCTTTTCTTTTTTTGGAATTTTGCAGGCAATAGATCAAAATAAGTTGTTTATAATTGATTATCATGATATCTACCTTCCATTTTTGGAAAGGATCAATGCCTTGGATGGTAGAAAATCATATGCCACACGTACCATATACTTTTTGACACCACTTGGTACTCTAAAGCCTGTTGCAATTGAACTTAGCCTGCCTCCATCTGGACCAAACACTCGATCAAAACGTGTAGTTACACCTGCTTTAGATGCAACTACTAATTGGGTGTGGATGCTTGCAAAAGCTCATGTTTGCTCCAATGATGCTGGTGTGCACCAACTTGCCCACCATTGGTAAGCAAGCATACAATTTTTCATTTACTTAGTCAATCATCTATTTAGTCCTAAAATTTTATGTGACAAGTGACACAAGACATTTACACTCACACGTGGACACTGATAATAATTTAATGAAATAATTGAATATAATCACATATGTTGTATCAAATTGACATGTGTCGGACACCAAATATGTCTTTCATGTGAAAAGACATTGCGACATAGGTTTGAAAAAATTGTTTAGAAATTATTTTGCAATTTTGTTAGTTTCATTGACACAATACCTTGACACATGCAACTTAAATACTTAATTGGTGATTGAAGTACTAGAACTATTTTTATATAACTGACATGAACTTATTTCTTTGCTTATTTTTTCTCGGTGAATTTTTATATAGGCTACGCACACATGCTTGCATGGAACCATTTATATTGTCTGCTCATAGGCAATTAAGTGCAATGCATCCAATTTTCAAGTTATTGGATCCACACATGAGGTACACTTTGGAGATCAATGCTTTAGCTCGACAGAGCTTGATCAATGCAGATGGTGTCATTGAGTCTTGCTTCACTCCTGGTCGCTATGCCATGGAGATTAGTTCTGCTGCTTACAAAACCAACTGGCGTTTTGACCAGGATAGCCTCCCTCAAGATCTCATCCGCAGGTAAATATCATATATTAATTTCGGCATTTTTTTTCCGCTTTGTTTTAAAGATCCTAGAACGAAACTCACACACGCAATACGGAGAATAGACATATTTTTTGCTTCTAACCTCTGTACATCAATTGTTTTCGCTGCCTTTTACCATTTGAGGCGCATTGTTGGTTCGTTATAACTTATTAGTTTATGACCTCAACTCTAAAGTTTAAAATAAGGGTCTGTGATTGTGGTCTGTGATTGTATCAGCTTCGTTTAACTACGATTCTTTGCAATATTAAGGATCGTGACATAACCACTCTCTAAAACCCTCCTCAACTATATTTTGATACCTGAGAGAAATTGTAAATATTAAACTTTCTTTTATGATTTGTTTGAGGCGTCACTTAGTATATGTTTGATTTCATTTTAGTCAAAATTGATTCTAGAAGTGTATAATTGTTTTTTATATGTTTGAGTGCATTTCGGAATAATTGATTTTGACTCTAGAATTGATTTTAATTTGAAACTATGATTTATAGTTTTTGACTCTAAACGTTAATTTTACACTTAAATTTATTGTTCAATCCACTATATGTGAATTTGTCCAAACGTAAACCATTTTACATTCAACTTAGTTTTAACTAAAATCAATTTTTCTCACCATAAAATCAAATACACACCTAGCTTCTACGGAAAAGAGACCCTACACTTTTCTTAACATGTAATTGATGTTTGCTTTATTTGGTGCAAGCATATGACATATAAATCTAACTTAAGATTTTCACTTTTTAACATTTCAGGGGAATGGCAGTACCAGACCCAACACAACCACATGGTCTAAAGCTAATAATGAAAGATTACCCTTATGCAGAAGATGGTCTTCTAATTTGGTCAGCAATAGAAAATTGGGTCCGCACTTATGTGAACTATTACTACCCAAATCCAAGCCTAATAATCAATGACAGAGAGTTACAAGCTTGGTACTCCGAATCAATCAATGTAGGCCACGCCGATATGAAAGACGAACCATGGTGGCCGAAATTGAACGACAGTGACAATCTTGTCCAAGTCCTAACAATCTTAATTTGGAATGCATCCGCGCAACATGCTGCACTTAATTTCGGACAGTATCCTTATGGAGGTTACGTGCCAAATCGTCCCCCGTTGATGAGAAGATTAATACCGGAAGAAAATGACCCTGAATATACAAGTTTCATATCAGATCCACAAAAGTATTTTCTCAATGCATTGCCTAGTTTGTTGCAAGCTACAAAATATATGGCTGTTGTTGACACGCTTTCGACTCATTCGTCTGACGAAGAGTATTTAGGTGAGAGGCAACAACCTTCGATTTGGACAGGTGATGCTGAGATTGTCGAAGCATTTTATCAATTCTCTGCGGAAATTGGAAAGATTGAGAAGGTGATTGATAGTAGGAATGGCGATAGGAATTTGAGGAACCGTTGCGGTGCTGGTGTTTTGCCGTATGAATTACTTGCTCCTAGTTCTGGACCTGGTGTTACATGCAGAGGGGTTCCAAATAGTGTGTCCACATAA
Protein sequence

>MS.gene30403.t1

MALTKEFMGSSLVERPIFPSSSPCFQQKNGGFLISPVFVPYGNKRVVRLRKSAKFPVAAISEDLMKNSSSPSSISSSSSSVPAEKPVKFKVRAVVTVRNKIKEDFKETFVKHLDAFTDRIGRNVVLELFSTEIDPKTKAAKKTNEAVLKDWSKKTNIKAERVNYTAEFTVDSNFGEPGAITVINNHQQEFYLENITIEGFATGAFHFPCNSWVQARKDLPGKRIFFSNKPYLPDDTPAGIKLLREKDLKKLRGDGKGVRKLSDRIYDYDTYNDLGNPDKGIDLARPTLGGSEMYPYPRRCRTGREPSDTDITCESRVEKPLPMYIPRDERFEESKMNTFSVKRLKGVLHNLLPGLKSSLSAQNRDFNEFSDVDGLYSVGLLIKLGLQDDILKKLPLPHIVSKIQESTSQGILKYDIPKIISKDKFAWLRDDEFARQAIAGVNPVTIERLTVFPPVSKLDPEIYGPQESALKEEHILNQLNGMTVQEAIDQNKLFIIDYHDIYLPFLERINALDGRKSYATRTIYFLTPLGTLKPVAIELSLPPSGPNTRSKRVVTPALDATTNWVWMLAKAHVCSNDAGVHQLAHHWLRTHACMEPFILSAHRQLSAMHPIFKLLDPHMRYTLEINALARQSLINADGVIESCFTPGRYAMEISSAAYKTNWRFDQDSLPQDLIRRGMAVPDPTQPHGLKLIMKDYPYAEDGLLIWSAIENWVRTYVNYYYPNPSLIINDRELQAWYSESINVGHADMKDEPWWPKLNDSDNLVQVLTILIWNASAQHAALNFGQYPYGGYVPNRPPLMRRLIPEENDPEYTSFISDPQKYFLNALPSLLQATKYMAVVDTLSTHSSDEEYLGERQQPSIWTGDAEIVEAFYQFSAEIGKIEKVIDSRNGDRNLRNRCGAGVLPYELLAPSSGPGVTCRGVPNSVST