Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30630.t1 | AFK43139.1 | 93.5 | 124 | 8 | 0 | 1 | 124 | 1 | 124 | 8.70E-59 | 236.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30630.t1 | O03982 | 64.5 | 76 | 26 | 1 | 1 | 75 | 1 | 76 | 1.5e-19 | 97.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30630.t1 | I3SS97 | 93.5 | 124 | 8 | 0 | 1 | 124 | 1 | 124 | 6.3e-59 | 236.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05410 | MS.gene30630 | 0.803038 | 4.22E-49 | -1.69E-46 |
| MS.gene058526 | MS.gene30630 | 0.865551 | 5.44E-65 | -1.69E-46 |
| MS.gene058527 | MS.gene30630 | 0.860129 | 2.56E-63 | -1.69E-46 |
| MS.gene058528 | MS.gene30630 | 0.865035 | 7.90E-65 | -1.69E-46 |
| MS.gene061047 | MS.gene30630 | 0.803664 | 3.13E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30630.t1 | MTR_6g049280 | 91.935 | 124 | 10 | 0 | 1 | 124 | 1 | 124 | 3.56e-73 | 214 |
| MS.gene30630.t1 | MTR_6g049280 | 90.323 | 124 | 12 | 0 | 1 | 124 | 31 | 154 | 1.97e-72 | 213 |
| MS.gene30630.t1 | MTR_6g049320 | 91.935 | 124 | 10 | 0 | 1 | 124 | 1 | 124 | 2.71e-72 | 211 |
| MS.gene30630.t1 | MTR_6g049320 | 90.323 | 124 | 12 | 0 | 1 | 124 | 31 | 154 | 7.50e-72 | 211 |
| MS.gene30630.t1 | MTR_6g051690 | 58.915 | 129 | 46 | 3 | 1 | 124 | 1 | 127 | 3.30e-38 | 125 |
| MS.gene30630.t1 | MTR_7g451200 | 55.556 | 108 | 46 | 1 | 1 | 108 | 1 | 106 | 8.80e-38 | 124 |
| MS.gene30630.t1 | MTR_7g451210 | 57.944 | 107 | 43 | 1 | 1 | 107 | 1 | 105 | 6.67e-37 | 122 |
| MS.gene30630.t1 | MTR_1173s0010 | 55.140 | 107 | 46 | 1 | 1 | 107 | 1 | 105 | 1.18e-36 | 121 |
| MS.gene30630.t1 | MTR_3g434890 | 56.075 | 107 | 45 | 1 | 1 | 107 | 1 | 105 | 5.15e-36 | 119 |
| MS.gene30630.t1 | MTR_1g099800 | 54.815 | 135 | 49 | 2 | 1 | 123 | 1 | 135 | 8.24e-36 | 120 |
| MS.gene30630.t1 | MTR_3g434960 | 55.140 | 107 | 46 | 1 | 1 | 107 | 1 | 105 | 1.62e-35 | 118 |
| MS.gene30630.t1 | MTR_6g049260 | 57.944 | 107 | 43 | 2 | 1 | 107 | 1 | 105 | 1.77e-35 | 118 |
| MS.gene30630.t1 | MTR_1g099810 | 51.799 | 139 | 50 | 3 | 1 | 123 | 1 | 138 | 5.56e-35 | 117 |
| MS.gene30630.t1 | MTR_1g099815 | 54.074 | 135 | 46 | 3 | 1 | 123 | 1 | 131 | 1.06e-34 | 117 |
| MS.gene30630.t1 | MTR_6g051680 | 75.714 | 70 | 17 | 0 | 1 | 70 | 1 | 70 | 2.33e-33 | 112 |
| MS.gene30630.t1 | MTR_8g098915 | 48.855 | 131 | 56 | 3 | 1 | 124 | 1 | 127 | 6.85e-33 | 112 |
| MS.gene30630.t1 | MTR_3g435000 | 52.475 | 101 | 46 | 1 | 6 | 106 | 10 | 108 | 5.67e-32 | 109 |
| MS.gene30630.t1 | MTR_1g099640 | 47.015 | 134 | 57 | 2 | 1 | 124 | 1 | 130 | 1.91e-31 | 108 |
| MS.gene30630.t1 | MTR_1g102240 | 51.111 | 135 | 50 | 4 | 1 | 123 | 1 | 131 | 2.20e-31 | 108 |
| MS.gene30630.t1 | MTR_0795s0020 | 50.746 | 134 | 49 | 3 | 1 | 124 | 1 | 127 | 1.13e-28 | 101 |
| MS.gene30630.t1 | MTR_1g099810 | 50.833 | 120 | 42 | 3 | 20 | 123 | 1 | 119 | 2.09e-26 | 95.5 |
| MS.gene30630.t1 | MTR_7g079110 | 61.765 | 68 | 26 | 0 | 1 | 68 | 1 | 68 | 4.05e-24 | 91.3 |
| MS.gene30630.t1 | MTR_3g434920 | 53.750 | 80 | 35 | 1 | 28 | 107 | 2 | 79 | 4.07e-23 | 86.3 |
| MS.gene30630.t1 | MTR_8g098915 | 45.536 | 112 | 50 | 3 | 20 | 124 | 1 | 108 | 1.69e-22 | 85.1 |
| MS.gene30630.t1 | MTR_1g099810 | 45.192 | 104 | 40 | 3 | 36 | 123 | 1 | 103 | 9.25e-16 | 67.8 |
| MS.gene30630.t1 | MTR_8g028615 | 66.176 | 68 | 23 | 0 | 1 | 68 | 1 | 68 | 6.14e-15 | 67.0 |
| MS.gene30630.t1 | MTR_1g099640 | 40.404 | 99 | 45 | 2 | 36 | 124 | 1 | 95 | 6.21e-14 | 63.2 |
| MS.gene30630.t1 | MTR_3g082250 | 43.210 | 81 | 42 | 4 | 3 | 79 | 5 | 85 | 1.00e-13 | 63.2 |
| MS.gene30630.t1 | MTR_5g020960 | 31.373 | 153 | 73 | 4 | 3 | 123 | 4 | 156 | 5.01e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30630.t1 | AT1G01490 | 66.197 | 71 | 23 | 1 | 1 | 70 | 1 | 71 | 3.26e-25 | 94.4 |
| MS.gene30630.t1 | AT1G01490 | 66.197 | 71 | 23 | 1 | 1 | 70 | 1 | 71 | 3.26e-25 | 94.4 |
| MS.gene30630.t1 | AT1G01490 | 65.714 | 70 | 23 | 1 | 2 | 70 | 29 | 98 | 1.13e-24 | 93.6 |
| MS.gene30630.t1 | AT5G52740 | 57.143 | 70 | 28 | 1 | 1 | 70 | 1 | 68 | 5.29e-19 | 76.6 |
| MS.gene30630.t1 | AT5G52760 | 43.269 | 104 | 49 | 4 | 5 | 105 | 7 | 103 | 7.13e-16 | 68.9 |
| MS.gene30630.t1 | AT5G52750 | 45.455 | 66 | 36 | 0 | 2 | 67 | 4 | 69 | 4.43e-13 | 62.0 |
| MS.gene30630.t1 | AT5G23760 | 50.725 | 69 | 33 | 1 | 3 | 70 | 4 | 72 | 9.30e-11 | 55.1 |
Find 0 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTGAAATAATTAAGTAT+TGG | - | 43378:323872-323891 | MS.gene30630:intron | 10.0% |
| !! | CCTTGATATATTTAATATAT+GGG | + | 43378:324207-324226 | None:intergenic | 15.0% |
| !! | TAATAATATGTTTGTATACA+CGG | - | 43378:324240-324259 | MS.gene30630:intron | 15.0% |
| !! | TTTGAACGAATAATATATTA+AGG | - | 43378:324006-324025 | MS.gene30630:intron | 15.0% |
| !!! | AAAAAATTGATAGTAAAAGA+GGG | + | 43378:323414-323433 | None:intergenic | 15.0% |
| !!! | TTATTTTGCTAAAATTGTAT+TGG | - | 43378:324345-324364 | MS.gene30630:intron | 15.0% |
| !! | CCCATATATTAAATATATCA+AGG | - | 43378:324204-324223 | MS.gene30630:intron | 20.0% |
| !! | CGGAAACATTTAATATATTA+AGG | - | 43378:323977-323996 | MS.gene30630:intron | 20.0% |
| !! | GCCTTGATATATTTAATATA+TGG | + | 43378:324208-324227 | None:intergenic | 20.0% |
| !! | TAGTTAAACATACCATAAAA+GGG | - | 43378:324051-324070 | MS.gene30630:intron | 20.0% |
| !! | TATTGGTATATTATTTACAG+GGG | - | 43378:324362-324381 | MS.gene30630:intron | 20.0% |
| !! | TTGTTGCATATGATAATAAT+GGG | + | 43378:324586-324605 | None:intergenic | 20.0% |
| !!! | GAAAAAATTGATAGTAAAAG+AGG | + | 43378:323415-323434 | None:intergenic | 20.0% |
| !!! | GTATTGGTATATTATTTACA+GGG | - | 43378:324361-324380 | MS.gene30630:intron | 20.0% |
| !!! | TCAAAAATAGTTTCCTTTTA+CGG | + | 43378:324085-324104 | None:intergenic | 20.0% |
| !!! | TGTATTGGTATATTATTTAC+AGG | - | 43378:324360-324379 | MS.gene30630:intron | 20.0% |
| ! | ATGATATTCAAATTCTGTTG+AGG | + | 43378:323761-323780 | None:intergenic | 25.0% |
| ! | CAGGAATATTGAATATATTG+TGG | + | 43378:324609-324628 | None:intergenic | 25.0% |
| ! | CTAGTTAAACATACCATAAA+AGG | - | 43378:324050-324069 | MS.gene30630:intron | 25.0% |
| ! | GTTGTTGCATATGATAATAA+TGG | + | 43378:324587-324606 | None:intergenic | 25.0% |
| ! | TACATGTAGTAAGTAAACTA+AGG | - | 43378:324448-324467 | MS.gene30630:intron | 25.0% |
| ! | TAGTAAGTAAACTAAGGAAA+TGG | - | 43378:324454-324473 | MS.gene30630:intron | 25.0% |
| ! | TGTTGCATATGATAATAATG+GGG | + | 43378:324585-324604 | None:intergenic | 25.0% |
| !! | ACTTGTCTAGTTTCAATATT+TGG | - | 43378:323562-323581 | MS.gene30630:CDS | 25.0% |
| AAGGGAATTATTACCGTAAA+AGG | - | 43378:324069-324088 | MS.gene30630:intron | 30.0% | |
| AATATATGGGAAATGCTAAC+CGG | + | 43378:324194-324213 | None:intergenic | 30.0% | |
| AGGCTTCAAGTAATTGAATT+TGG | + | 43378:324560-324579 | None:intergenic | 30.0% | |
| AGTTTACTTACTACATGTAC+TGG | + | 43378:324447-324466 | None:intergenic | 30.0% | |
| ATATTCCTGTGTTTCAAGAA+TGG | - | 43378:324620-324639 | MS.gene30630:intron | 30.0% | |
| ATGCTGAAATAGTTTCTGTT+GGG | - | 43378:324481-324500 | MS.gene30630:intron | 30.0% | |
| GACAAGAAATTAACCATAAC+TGG | - | 43378:324411-324430 | MS.gene30630:intron | 30.0% | |
| ! | ACGGTAATAATTCCCTTTTA+TGG | + | 43378:324066-324085 | None:intergenic | 30.0% |
| ! | CATATGATAATAATGGGGAT+AGG | + | 43378:324580-324599 | None:intergenic | 30.0% |
| ! | GTAACAGAAACTAGTATTGA+AGG | - | 43378:323470-323489 | MS.gene30630:CDS | 30.0% |
| ! | TTCTTCTTTTCATCTTTTGC+AGG | + | 43378:324507-324526 | None:intergenic | 30.0% |
| !! | ATCGTTTTTCACTGTTTATG+AGG | - | 43378:323714-323733 | MS.gene30630:intron | 30.0% |
| ACTCAAGTGGTAAAAGATTG+CGG | - | 43378:323957-323976 | MS.gene30630:intron | 35.0% | |
| AGTAAAAGAGGGAATTCACA+AGG | + | 43378:323403-323422 | None:intergenic | 35.0% | |
| CAAATTCTGTTGAGGTTACT+AGG | + | 43378:323753-323772 | None:intergenic | 35.0% | |
| CATGCTGAAATAGTTTCTGT+TGG | - | 43378:324480-324499 | MS.gene30630:intron | 35.0% | |
| TTGTTCCATTCTTGAAACAC+AGG | + | 43378:324628-324647 | None:intergenic | 35.0% | |
| ! | ACAAGTTGACATTACCAGAA+AGG | + | 43378:323548-323567 | None:intergenic | 35.0% |
| ! | ACAGAAACTAGTATTGAAGG+TGG | - | 43378:323473-323492 | MS.gene30630:CDS | 35.0% |
| ! | AGTCTTCATAGCTTTTTGCT+TGG | + | 43378:323517-323536 | None:intergenic | 35.0% |
| ! | GTTTTTCACTGTTTATGAGG+TGG | - | 43378:323717-323736 | MS.gene30630:intron | 35.0% |
| !! | ATTTGGTTCTTCTTCACATC+AGG | + | 43378:324543-324562 | None:intergenic | 35.0% |
| AAAGCTATGAAGACTGTCTC+AGG | - | 43378:323522-323541 | MS.gene30630:CDS | 40.0% | |
| AGAATGGAACAAGATCCTGT+TGG | - | 43378:324636-324655 | MS.gene30630:intron | 40.0% | |
| CAGCATATGATACATCCAAC+AGG | + | 43378:324654-324673 | None:intergenic | 40.0% | |
| CGACATAAGTGTAACTCAAG+TGG | - | 43378:323944-323963 | MS.gene30630:intron | 40.0% | |
| TATATCCTTAACCAGTGTCC+CGG | - | 43378:324164-324183 | MS.gene30630:intron | 40.0% | |
| TTAAGGTCTAATTGTGCCCT+CGG | - | 43378:324023-324042 | MS.gene30630:intron | 40.0% | |
| ATATCCTTAACCAGTGTCCC+GGG | - | 43378:324165-324184 | MS.gene30630:intron | 45.0% | |
| ATGTTTAACTAGTGCCCCGA+GGG | + | 43378:324042-324061 | None:intergenic | 45.0% | |
| TAAGGTCTAATTGTGCCCTC+GGG | - | 43378:324024-324043 | MS.gene30630:intron | 45.0% | |
| TATGTTTAACTAGTGCCCCG+AGG | + | 43378:324043-324062 | None:intergenic | 45.0% | |
| TGGATCTGTGTCTCCAGTTA+TGG | + | 43378:324427-324446 | None:intergenic | 45.0% | |
| !!! | TATTTATTTTAATTGAAAAA+TGG | + | 43378:323810-323829 | None:intergenic | 5.0% |
| AAGACTGTCTCAGGCCTTTC+TGG | - | 43378:323531-323550 | MS.gene30630:CDS | 50.0% | |
| AAGGTCTAATTGTGCCCTCG+GGG | - | 43378:324025-324044 | MS.gene30630:intron | 50.0% | |
| GAAATGCTAACCGGTGTCCC+GGG | + | 43378:324185-324204 | None:intergenic | 55.0% | |
| GGAAATGCTAACCGGTGTCC+CGG | + | 43378:324186-324205 | None:intergenic | 55.0% | |
| TAACCAGTGTCCCGGGACAC+CGG | - | 43378:324172-324191 | MS.gene30630:intron | 60.0% | |
| ! | GTGTCCCGGGACACTGGTTA+AGG | + | 43378:324172-324191 | None:intergenic | 60.0% |
| TAACCGGTGTCCCGGGACAC+TGG | + | 43378:324178-324197 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43378 | gene | 323381 | 324674 | 323381 | ID=MS.gene30630 |
| 43378 | mRNA | 323381 | 324674 | 323381 | ID=MS.gene30630.t1;Parent=MS.gene30630 |
| 43378 | exon | 324669 | 324674 | 324669 | ID=MS.gene30630.t1.exon1;Parent=MS.gene30630.t1 |
| 43378 | CDS | 324669 | 324674 | 324669 | ID=cds.MS.gene30630.t1;Parent=MS.gene30630.t1 |
| 43378 | exon | 324503 | 324578 | 324503 | ID=MS.gene30630.t1.exon2;Parent=MS.gene30630.t1 |
| 43378 | CDS | 324503 | 324578 | 324503 | ID=cds.MS.gene30630.t1;Parent=MS.gene30630.t1 |
| 43378 | exon | 323381 | 323673 | 323381 | ID=MS.gene30630.t1.exon3;Parent=MS.gene30630.t1 |
| 43378 | CDS | 323381 | 323673 | 323381 | ID=cds.MS.gene30630.t1;Parent=MS.gene30630.t1 |
| Gene Sequence |
| Protein sequence |
