AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061047


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061047.t1 MTR_1g034300 95.918 196 8 0 5 200 676 871 5.50e-123 368
MS.gene061047.t1 MTR_1g034300 95.918 196 8 0 5 200 675 870 6.24e-123 368
MS.gene061047.t1 MTR_5g018510 50.262 191 93 1 4 192 711 901 8.36e-64 212
MS.gene061047.t1 MTR_5g018510 50.526 190 92 1 5 192 711 900 1.04e-63 211
MS.gene061047.t1 MTR_8g079010 51.685 178 84 1 5 180 588 765 5.57e-60 200
MS.gene061047.t1 MTR_6g071360 44.828 174 84 2 5 176 687 850 2.13e-46 163
MS.gene061047.t1 MTR_5g027970 43.820 178 87 3 5 177 644 813 2.82e-44 157
MS.gene061047.t1 MTR_1g015750 28.758 153 84 4 18 170 226 353 1.79e-17 79.7
MS.gene061047.t1 MTR_1g015750 28.758 153 84 4 18 170 234 361 2.15e-17 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061047.t1 AT4G34390 51.456 206 81 4 5 200 625 821 2.40e-58 196
MS.gene061047.t1 AT4G34390 51.456 206 81 4 5 200 664 860 3.29e-58 196
MS.gene061047.t1 AT2G23460 47.872 188 91 2 5 192 701 881 4.03e-55 187
MS.gene061047.t1 AT1G31930 42.857 189 95 3 5 188 651 831 4.08e-44 156
MS.gene061047.t1 AT1G31930 42.857 189 95 3 5 188 651 831 4.08e-44 156
MS.gene061047.t1 AT1G31930 42.857 189 95 3 5 188 651 831 4.08e-44 156
MS.gene061047.t1 AT1G31930 42.857 189 95 3 5 188 651 831 4.08e-44 156
MS.gene061047.t1 AT1G31930 42.857 189 95 3 5 188 651 831 4.08e-44 156
MS.gene061047.t1 AT1G31930 42.857 189 95 3 5 188 651 831 4.08e-44 156
MS.gene061047.t1 AT2G26300 27.451 153 86 3 18 170 225 352 1.68e-18 82.8
MS.gene061047.t1 AT2G26300 27.451 153 86 3 18 170 225 352 1.68e-18 82.8

Find 30 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTTCTTCTTCTTGAAATT+TGG 0.098655 1.4:-18575748 None:intergenic
CTATTTGTTTCTTTGGTTAC+TGG 0.199983 1.4:+18575985 MS.gene061047:CDS
AAGAACTCTATTTGTTTCTT+TGG 0.238160 1.4:+18575978 MS.gene061047:CDS
TTCCTCAAGAAGATCGAATT+TGG 0.241366 1.4:-18575785 None:intergenic
AACCAGTCACACCTTGTTAA+TGG 0.243332 1.4:-18575823 None:intergenic
AGAGCTTCATCGATCGTATC+AGG 0.331731 1.4:-18576015 None:intergenic
CTAATGAGTTGGTATCTGTT+AGG 0.342148 1.4:-18575547 None:intergenic
AGAAACAAATAGAGTTCTTT+CGG 0.367235 1.4:-18575974 None:intergenic
TTGTGATTGTTACTTATAAC+CGG 0.382473 1.4:-18575859 None:intergenic
GACTTATCATTAACAATAGA+AGG 0.384451 1.4:-18576078 None:intergenic
ATCGATGAAGCTCTTCGATA+TGG 0.397848 1.4:+18576024 MS.gene061047:CDS
GTACTCATCGTAGTCGGTTA+AGG 0.438650 1.4:-18575650 None:intergenic
TTCCATTAACAAGGTGTGAC+TGG 0.468855 1.4:+18575821 MS.gene061047:CDS
TAATGAGTTGGTATCTGTTA+GGG 0.471976 1.4:-18575546 None:intergenic
TGAAGTACTTGCATCTCCAT+TGG 0.478445 1.4:-18576118 None:intergenic
TGGTAGAGAGGTTATTGAAT+GGG 0.498729 1.4:+18576044 MS.gene061047:CDS
AAAGCACTTTGAGCTAATGA+AGG 0.521537 1.4:-18575907 None:intergenic
TTGGATGTACTCTAATGAGT+TGG 0.523296 1.4:-18575558 None:intergenic
CACCAAATTCGATCTTCTTG+AGG 0.526416 1.4:+18575783 MS.gene061047:CDS
ATGGTAGAGAGGTTATTGAA+TGG 0.529478 1.4:+18576043 MS.gene061047:CDS
TGGAGAAAACTGCAAGTGGT+TGG 0.551701 1.4:+18575588 MS.gene061047:CDS
GAGATTACTTCAACAACCAA+TGG 0.567210 1.4:+18576102 MS.gene061047:CDS
ATCGTAGTCGGTTAAGGAGA+CGG 0.579802 1.4:-18575644 None:intergenic
AGTTGCTACAAACAAAATGT+TGG 0.587657 1.4:+18575690 MS.gene061047:CDS
GAGTACATTATAGACAGTAA+AGG 0.599991 1.4:+18575667 MS.gene061047:CDS
TCCTTGGAGAAAACTGCAAG+TGG 0.617052 1.4:+18575584 MS.gene061047:CDS
TCGAACATGTTCCATTAACA+AGG 0.629726 1.4:+18575812 MS.gene061047:CDS
TATAATGTACTCATCGTAGT+CGG 0.631790 1.4:-18575656 None:intergenic
GACGGAGAACAAAATAACAT+CGG 0.680660 1.4:-18575626 None:intergenic
AGCTCTTCGATATGGTAGAG+AGG 0.683781 1.4:+18576032 MS.gene061047:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTCTTCTTCTTGAAATT+TGG - chr1.4:18575751-18575770 None:intergenic 20.0%
! AGAAACAAATAGAGTTCTTT+CGG - chr1.4:18575977-18575996 None:intergenic 25.0%
! AGTAACAATCACAAAAAATG+TGG + chr1.4:18575868-18575887 MS.gene061047:CDS 25.0%
! GACTTATCATTAACAATAGA+AGG - chr1.4:18576081-18576100 None:intergenic 25.0%
!! AAGAACTCTATTTGTTTCTT+TGG + chr1.4:18575978-18575997 MS.gene061047:CDS 25.0%
!! TTGTGATTGTTACTTATAAC+CGG - chr1.4:18575862-18575881 None:intergenic 25.0%
AGGTTATTGAATGGGAAAAA+TGG + chr1.4:18576052-18576071 MS.gene061047:CDS 30.0%
AGTTGCTACAAACAAAATGT+TGG + chr1.4:18575690-18575709 MS.gene061047:CDS 30.0%
CACAAAAAATGTGGTAACAA+TGG + chr1.4:18575877-18575896 MS.gene061047:CDS 30.0%
GAGTACATTATAGACAGTAA+AGG + chr1.4:18575667-18575686 MS.gene061047:CDS 30.0%
GGTTATTGAATGGGAAAAAT+GGG + chr1.4:18576053-18576072 MS.gene061047:CDS 30.0%
TATAATGTACTCATCGTAGT+CGG - chr1.4:18575659-18575678 None:intergenic 30.0%
! CTATTTGTTTCTTTGGTTAC+TGG + chr1.4:18575985-18576004 MS.gene061047:CDS 30.0%
! GAGCAATGATGTTTTCAAAT+AGG - chr1.4:18575726-18575745 None:intergenic 30.0%
!! TAATGAGTTGGTATCTGTTA+GGG - chr1.4:18575549-18575568 None:intergenic 30.0%
AGAGTACATCCAAAAATCCT+TGG + chr1.4:18575568-18575587 MS.gene061047:CDS 35.0%
ATGGTAGAGAGGTTATTGAA+TGG + chr1.4:18576043-18576062 MS.gene061047:CDS 35.0%
GACGGAGAACAAAATAACAT+CGG - chr1.4:18575629-18575648 None:intergenic 35.0%
GAGATTACTTCAACAACCAA+TGG + chr1.4:18576102-18576121 MS.gene061047:CDS 35.0%
TCGAACATGTTCCATTAACA+AGG + chr1.4:18575812-18575831 MS.gene061047:CDS 35.0%
TGGTAGAGAGGTTATTGAAT+GGG + chr1.4:18576044-18576063 MS.gene061047:CDS 35.0%
TTCCTCAAGAAGATCGAATT+TGG - chr1.4:18575788-18575807 None:intergenic 35.0%
TTGGATGTACTCTAATGAGT+TGG - chr1.4:18575561-18575580 None:intergenic 35.0%
! AAAGCACTTTGAGCTAATGA+AGG - chr1.4:18575910-18575929 None:intergenic 35.0%
! CAGTTTTCTCCAAGGATTTT+TGG - chr1.4:18575580-18575599 None:intergenic 35.0%
!! CTAATGAGTTGGTATCTGTT+AGG - chr1.4:18575550-18575569 None:intergenic 35.0%
!!! CTGGTTTTGTGAATTCAATC+CGG + chr1.4:18575840-18575859 MS.gene061047:CDS 35.0%
AACCAGTCACACCTTGTTAA+TGG - chr1.4:18575826-18575845 None:intergenic 40.0%
ATCGATGAAGCTCTTCGATA+TGG + chr1.4:18576024-18576043 MS.gene061047:CDS 40.0%
CACCAAATTCGATCTTCTTG+AGG + chr1.4:18575783-18575802 MS.gene061047:CDS 40.0%
TTCCATTAACAAGGTGTGAC+TGG + chr1.4:18575821-18575840 MS.gene061047:CDS 40.0%
! TGAAGTACTTGCATCTCCAT+TGG - chr1.4:18576121-18576140 None:intergenic 40.0%
AGAGCTTCATCGATCGTATC+AGG - chr1.4:18576018-18576037 None:intergenic 45.0%
AGCTCTTCGATATGGTAGAG+AGG + chr1.4:18576032-18576051 MS.gene061047:CDS 45.0%
ATCGTAGTCGGTTAAGGAGA+CGG - chr1.4:18575647-18575666 None:intergenic 45.0%
GTACTCATCGTAGTCGGTTA+AGG - chr1.4:18575653-18575672 None:intergenic 45.0%
TCCTTGGAGAAAACTGCAAG+TGG + chr1.4:18575584-18575603 MS.gene061047:CDS 45.0%
TGGAGAAAACTGCAAGTGGT+TGG + chr1.4:18575588-18575607 MS.gene061047:CDS 45.0%
! ACCACTTGCAGTTTTCTCCA+AGG - chr1.4:18575588-18575607 None:intergenic 45.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 18575541 18576143 18575541 ID=MS.gene061047
chr1.4 mRNA 18575541 18576143 18575541 ID=MS.gene061047.t1;Parent=MS.gene061047
chr1.4 exon 18575541 18576143 18575541 ID=MS.gene061047.t1.exon1;Parent=MS.gene061047.t1
chr1.4 CDS 18575541 18576143 18575541 ID=cds.MS.gene061047.t1;Parent=MS.gene061047.t1
Gene Sequence

>MS.gene061047

ATGTTCCCTAACAGATACCAACTCATTAGAGTACATCCAAAAATCCTTGGAGAAAACTGCAAGTGGTTGGAGATGTTTGAAGACACCGATGTTATTTTGTTCTCCGTCTCCTTAACCGACTACGATGAGTACATTATAGACAGTAAAGGAGTTGCTACAAACAAAATGTTGGCAGCAAAAAACCTATTTGAAAACATCATTGCTCATCCAAATTTCAAGAAGAAGAAATTTGTTTTAATACTCACCAAATTCGATCTTCTTGAGGAAAAGATCGAACATGTTCCATTAACAAGGTGTGACTGGTTTTGTGAATTCAATCCGGTTATAAGTAACAATCACAAAAAATGTGGTAACAATGGAACTATGCCTTCATTAGCTCAAAGTGCTTTTCAATACATTGCTATCAAGTTTAAGAGATTGTTTCATTCGTATACCGAAAGAACTCTATTTGTTTCTTTGGTTACTGGATTAGAACCTGATACGATCGATGAAGCTCTTCGATATGGTAGAGAGGTTATTGAATGGGAAAAATGGGATCCTTCTATTGTTAATGATAAGTCTGAGATTACTTCAACAACCAATGGAGATGCAAGTACTTCATGA

Protein sequence

>MS.gene061047.t1

MFPNRYQLIRVHPKILGENCKWLEMFEDTDVILFSVSLTDYDEYIIDSKGVATNKMLAAKNLFENIIAHPNFKKKKFVLILTKFDLLEEKIEHVPLTRCDWFCEFNPVISNNHKKCGNNGTMPSLAQSAFQYIAIKFKRLFHSYTERTLFVSLVTGLEPDTIDEALRYGREVIEWEKWDPSIVNDKSEITSTTNGDASTS