Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30687.t1 | XP_013452215.1 | 94.9 | 353 | 9 | 3 | 1 | 348 | 1 | 349 | 1.60E-166 | 595.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30687.t1 | Q9MBA2 | 80.5 | 323 | 38 | 2 | 27 | 348 | 28 | 326 | 2.0e-141 | 503.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30687.t1 | A0A072UAX9 | 94.9 | 353 | 9 | 3 | 1 | 348 | 1 | 349 | 1.1e-166 | 595.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055971 | MS.gene30687 | 0.806984 | 6.31E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30687.t1 | MTR_6g048300 | 95.184 | 353 | 8 | 3 | 1 | 348 | 1 | 349 | 0.0 | 665 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30687.t1 | AT5G24020 | 80.368 | 326 | 39 | 2 | 24 | 348 | 25 | 326 | 3.21e-176 | 492 |
Find 0 sgRNAs with CRISPR-Local
Find 150 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATTAAGATGATAGTGAAT+AGG | - | 42982:12400-12419 | MS.gene30687:CDS | 20.0% |
| !!! | TATTAAGATGATAGTGAATA+GGG | - | 42982:12401-12420 | MS.gene30687:CDS | 20.0% |
| ! | TCTAAATTACCTATTGGATT+TGG | - | 42982:12090-12109 | MS.gene30687:CDS | 25.0% |
| AAATTACCTATTGGATTTGG+TGG | - | 42982:12093-12112 | MS.gene30687:CDS | 30.0% | |
| CCAATAGGTAATTTAGATCT+AGG | + | 42982:12087-12106 | None:intergenic | 30.0% | |
| CCTAGATCTAAATTACCTAT+TGG | - | 42982:12084-12103 | MS.gene30687:CDS | 30.0% | |
| !! | GATTTTCAAGACCTAGTAAA+AGG | + | 42982:11957-11976 | None:intergenic | 30.0% |
| !!! | AAGAAGGGATTTTGTTGTTA+GGG | + | 42982:11761-11780 | None:intergenic | 30.0% |
| !!! | GATTTTGTTGTTAGGGTTTT+GGG | + | 42982:11754-11773 | None:intergenic | 30.0% |
| CAACAAAATCCCTTCTTCAA+TGG | - | 42982:11764-11783 | MS.gene30687:CDS | 35.0% | |
| CTTTGGTTAGAGATAAACGT+TGG | - | 42982:12031-12050 | MS.gene30687:CDS | 35.0% | |
| TAAAGGCGAAGATATGATGT+CGG | - | 42982:12440-12459 | MS.gene30687:CDS | 35.0% | |
| TCCCTTAATGCAGTTATATC+CGG | + | 42982:12336-12355 | None:intergenic | 35.0% | |
| TTTGAATAAGCCTCCTACTT+TGG | - | 42982:12560-12579 | MS.gene30687:CDS | 35.0% | |
| ! | AACGAGAAAAACCCTCTTTT+AGG | + | 42982:12663-12682 | None:intergenic | 35.0% |
| ! | TTATAAGGAGTACGAATCGA+GGG | - | 42982:12526-12545 | MS.gene30687:CDS | 35.0% |
| !! | AGAGGGTTTTTCTCGTTTTT+TGG | - | 42982:12666-12685 | MS.gene30687:CDS | 35.0% |
| !!! | GAAGAAGGGATTTTGTTGTT+AGG | + | 42982:11762-11781 | None:intergenic | 35.0% |
| !!! | GGATTTTGTTGTTAGGGTTT+TGG | + | 42982:11755-11774 | None:intergenic | 35.0% |
| AAAACCGGTGGAAATGGAAA+TGG | - | 42982:12162-12181 | MS.gene30687:CDS | 40.0% | |
| AGTTCCATTTCCATTTCCAC+CGG | + | 42982:12169-12188 | None:intergenic | 40.0% | |
| ATCAAAACCGCTTCATTAGC+AGG | + | 42982:12306-12325 | None:intergenic | 40.0% | |
| CAAGACCTAGTAAAAGGTCT+AGG | + | 42982:11951-11970 | None:intergenic | 40.0% | |
| CAATCGATCAGGATAAAGTC+CGG | + | 42982:12243-12262 | None:intergenic | 40.0% | |
| CTTATAACCTCACTATCCTC+CGG | + | 42982:12513-12532 | None:intergenic | 40.0% | |
| GCCGGATATAACTGCATTAA+GGG | - | 42982:12332-12351 | MS.gene30687:CDS | 40.0% | |
| GCTAGGACGGATATGATTAA+AGG | - | 42982:12423-12442 | MS.gene30687:CDS | 40.0% | |
| GGAGGCTTATTCAAAACAAG+TGG | + | 42982:12555-12574 | None:intergenic | 40.0% | |
| GGATAGTATGCAAGCTGTTA+TGG | - | 42982:12626-12645 | MS.gene30687:CDS | 40.0% | |
| TAAAGAAAGGCCGATGTTAG+CGG | + | 42982:11875-11894 | None:intergenic | 40.0% | |
| TATGCAAGCTGTTATGGTTG+AGG | - | 42982:12632-12651 | MS.gene30687:CDS | 40.0% | |
| TTGAGGAAGAGCCTAAAAGA+GGG | - | 42982:12649-12668 | MS.gene30687:CDS | 40.0% | |
| TTGGATGTGCAAGAGATGTT+GGG | - | 42982:12465-12484 | MS.gene30687:CDS | 40.0% | |
| TTTGCGGTTCCATTGAAGAA+GGG | + | 42982:11776-11795 | None:intergenic | 40.0% | |
| TTTGGATGTGCAAGAGATGT+TGG | - | 42982:12464-12483 | MS.gene30687:CDS | 40.0% | |
| ! | AGATGATAGTGAATAGGGCT+AGG | - | 42982:12406-12425 | MS.gene30687:CDS | 40.0% |
| ! | CGTAACCTAGACCTTTTACT+AGG | - | 42982:11943-11962 | MS.gene30687:CDS | 40.0% |
| ! | GCTTTTCCACCAAATCCAAT+AGG | + | 42982:12102-12121 | None:intergenic | 40.0% |
| ! | GTTATAAGGAGTACGAATCG+AGG | - | 42982:12525-12544 | MS.gene30687:CDS | 40.0% |
| ! | TTGGTGGAAAAGCTTTGACT+TGG | - | 42982:12109-12128 | MS.gene30687:CDS | 40.0% |
| !! | TGGAAAAGCTTTGACTTGGT+TGG | - | 42982:12113-12132 | MS.gene30687:CDS | 40.0% |
| !! | TGGTTGAAGCTTTAAAAGCC+CGG | - | 42982:12133-12152 | MS.gene30687:CDS | 40.0% |
| !!! | CGAAGATATGATGTCGGTTT+TGG | - | 42982:12446-12465 | MS.gene30687:CDS | 40.0% |
| !!! | GGGTTTTTCTCGTTTTTTGG+TGG | - | 42982:12669-12688 | MS.gene30687:CDS | 40.0% |
| !!! | GTTGTTAGGGTTTTGGGTTT+GGG | + | 42982:11748-11767 | None:intergenic | 40.0% |
| !!! | TGTTGTTAGGGTTTTGGGTT+TGG | + | 42982:11749-11768 | None:intergenic | 40.0% |
| AAAGCCAAACCAGCCAAAGT+AGG | + | 42982:12576-12595 | None:intergenic | 45.0% | |
| AAGGGATGCAGATAGAGTGA+CGG | - | 42982:12350-12369 | MS.gene30687:CDS | 45.0% | |
| AATAAGCCTCCTACTTTGGC+TGG | - | 42982:12564-12583 | MS.gene30687:CDS | 45.0% | |
| AATGGAACTGGAACCAATGG+TGG | - | 42982:12180-12199 | MS.gene30687:CDS | 45.0% | |
| ACCACTACAACCGCTAACAT+CGG | - | 42982:11862-11881 | MS.gene30687:CDS | 45.0% | |
| ACCGTCGTTGAGATTCTCAA+CGG | - | 42982:11988-12007 | MS.gene30687:CDS | 45.0% | |
| ATGGGTGATTGAAGGGAAGT+GGG | + | 42982:11698-11717 | None:intergenic | 45.0% | |
| ATGTGACTACTACAACGCGT+GGG | + | 42982:11819-11838 | None:intergenic | 45.0% | |
| CATTACGCCTGCTAATGAAG+CGG | - | 42982:12296-12315 | MS.gene30687:CDS | 45.0% | |
| CGCCGGATATAACTGCATTA+AGG | - | 42982:12331-12350 | MS.gene30687:CDS | 45.0% | |
| CGGAGGATAGTGAGGTTATA+AGG | - | 42982:12511-12530 | MS.gene30687:CDS | 45.0% | |
| GATAGTGAATAGGGCTAGGA+CGG | - | 42982:12410-12429 | MS.gene30687:CDS | 45.0% | |
| GATGGAGATGGGTGATTGAA+GGG | + | 42982:11705-11724 | None:intergenic | 45.0% | |
| GGAAATGGAACTGGAACCAA+TGG | - | 42982:12177-12196 | MS.gene30687:CDS | 45.0% | |
| GGAGATAAAACCGGTGGAAA+TGG | - | 42982:12156-12175 | MS.gene30687:CDS | 45.0% | |
| GGTGGAAATGGAAATGGAAC+TGG | - | 42982:12168-12187 | MS.gene30687:CDS | 45.0% | |
| GTAGTAGTCACATCCGGAAA+AGG | - | 42982:11826-11845 | MS.gene30687:CDS | 45.0% | |
| GTTGAGGAAGAGCCTAAAAG+AGG | - | 42982:12648-12667 | MS.gene30687:CDS | 45.0% | |
| GTTTGCGGTTCCATTGAAGA+AGG | + | 42982:11777-11796 | None:intergenic | 45.0% | |
| TAGGTTACGTAATCCGACGT+CGG | + | 42982:11932-11951 | None:intergenic | 45.0% | |
| TCCGGTATAACACCAAGCAA+CGG | + | 42982:12495-12514 | None:intergenic | 45.0% | |
| TGTTATACCGGAGGATAGTG+AGG | - | 42982:12503-12522 | MS.gene30687:CDS | 45.0% | |
| ! | AGATAGAGTGACGGGATTGT+TGG | - | 42982:12359-12378 | MS.gene30687:CDS | 45.0% |
| ! | AGGGAAGTGGGATTTGAGAT+TGG | + | 42982:11686-11705 | None:intergenic | 45.0% |
| ! | GAAGTGGGATTTGAGATTGG+TGG | + | 42982:11683-11702 | None:intergenic | 45.0% |
| ! | GTTGGAGTGTGATGGGATTA+GGG | - | 42982:12377-12396 | MS.gene30687:CDS | 45.0% |
| ! | TGGCTTTTGAACAAGCTGCT+TGG | - | 42982:12589-12608 | MS.gene30687:CDS | 45.0% |
| ! | TGTTGGAGTGTGATGGGATT+AGG | - | 42982:12376-12395 | MS.gene30687:CDS | 45.0% |
| ! | TTTACCAACTCCGCCTTTTC+CGG | + | 42982:11842-11861 | None:intergenic | 45.0% |
| !! | AGCCTAAACGGGCTAAAGAA+AGG | + | 42982:11888-11907 | None:intergenic | 45.0% |
| !! | AGCGGTTTTGATAACAACGC+CGG | - | 42982:12314-12333 | MS.gene30687:CDS | 45.0% |
| !! | GGTTGAAGCTTTAAAAGCCC+GGG | - | 42982:12134-12153 | MS.gene30687:CDS | 45.0% |
| !! | GTTGAAGCTTTAAAAGCCCG+GGG | - | 42982:12135-12154 | MS.gene30687:CDS | 45.0% |
| !! | TTGTCGACTCGATCAAGCTT+TGG | - | 42982:12014-12033 | MS.gene30687:CDS | 45.0% |
| !!! | AGGGTTTTGGGTTTGGGTTT+GGG | + | 42982:11742-11761 | None:intergenic | 45.0% |
| !!! | TAGGGTTTTGGGTTTGGGTT+TGG | + | 42982:11743-11762 | None:intergenic | 45.0% |
| AAAGCCCGGGGAGATAAAAC+CGG | - | 42982:12147-12166 | MS.gene30687:CDS | 50.0% | |
| ACAAGCTGCTTGGAGACTTG+TGG | - | 42982:12599-12618 | MS.gene30687:CDS | 50.0% | |
| ACGGGATTGTTGGAGTGTGA+TGG | - | 42982:12369-12388 | MS.gene30687:CDS | 50.0% | |
| ACTGGAACCAATGGTGGTTC+TGG | - | 42982:12186-12205 | MS.gene30687:CDS | 50.0% | |
| AGAACCAGTGGAACTGGAGT+TGG | + | 42982:12214-12233 | None:intergenic | 50.0% | |
| AGGGATGCAGATAGAGTGAC+GGG | - | 42982:12351-12370 | MS.gene30687:CDS | 50.0% | |
| ATGTTGGGTTTGCCGTTGCT+TGG | - | 42982:12480-12499 | MS.gene30687:CDS | 50.0% | |
| CGCGTTGTAGTAGTCACATC+CGG | - | 42982:11820-11839 | MS.gene30687:CDS | 50.0% | |
| CGCTTCATTAGCAGGCGTAA+TGG | + | 42982:12298-12317 | None:intergenic | 50.0% | |
| CGGGATTGTTGGAGTGTGAT+GGG | - | 42982:12370-12389 | MS.gene30687:CDS | 50.0% | |
| GATGGGTGATTGAAGGGAAG+TGG | + | 42982:11699-11718 | None:intergenic | 50.0% | |
| GATGTGACTACTACAACGCG+TGG | + | 42982:11820-11839 | None:intergenic | 50.0% | |
| GCCGTTGAGAATCTCAACGA+CGG | + | 42982:11992-12011 | None:intergenic | 50.0% | |
| GGATGGAGATGGGTGATTGA+AGG | + | 42982:11706-11725 | None:intergenic | 50.0% | |
| GGCCTTTCTTTAGCCCGTTT+AGG | - | 42982:11883-11902 | MS.gene30687:CDS | 50.0% | |
| GTAGTCACATCCGGAAAAGG+CGG | - | 42982:11829-11848 | MS.gene30687:CDS | 50.0% | |
| GTTGGAACCAGAACCACCAT+TGG | + | 42982:12196-12215 | None:intergenic | 50.0% | |
| TATCCTGATCGATTGCCCAG+CGG | - | 42982:12248-12267 | MS.gene30687:CDS | 50.0% | |
| TGTGACTACTACAACGCGTG+GGG | + | 42982:11818-11837 | None:intergenic | 50.0% | |
| ! | GGTTTGGGTTTGGGGAATGT+GGG | + | 42982:11733-11752 | None:intergenic | 50.0% |
| ! | GTGGGATTTGAGATTGGTGG+TGG | + | 42982:11680-11699 | None:intergenic | 50.0% |
| !! | AACTCCAGTTCCACTGGTTC+TGG | - | 42982:12213-12232 | MS.gene30687:CDS | 50.0% |
| !! | GCCGTTGCTTGGTGTTATAC+CGG | - | 42982:12491-12510 | MS.gene30687:CDS | 50.0% |
| !! | GTTGCTTGGTGTTATACCGG+AGG | - | 42982:12494-12513 | MS.gene30687:CDS | 50.0% |
| !! | TTTGAGATTGGTGGTGGTGC+CGG | + | 42982:11674-11693 | None:intergenic | 50.0% |
| !!! | GGGTTTTGGGTTTGGGTTTG+GGG | + | 42982:11741-11760 | None:intergenic | 50.0% |
| !!! | TTCCACCGGTTTTATCTCCC+CGG | + | 42982:12155-12174 | None:intergenic | 50.0% |
| AACCGCAAACCCCAACTCTC+CGG | - | 42982:11787-11806 | MS.gene30687:CDS | 55.0% | |
| ACAGCCAGAACCAGTGGAAC+TGG | + | 42982:12220-12239 | None:intergenic | 55.0% | |
| ACATCCGGAAAAGGCGGAGT+TGG | - | 42982:11835-11854 | MS.gene30687:CDS | 55.0% | |
| ATCCTGATCGATTGCCCAGC+GGG | - | 42982:12249-12268 | MS.gene30687:CDS | 55.0% | |
| ATGTGGGAGAGGGATGGAGA+TGG | + | 42982:11717-11736 | None:intergenic | 55.0% | |
| CGACGACGGAGAAGCCTAAA+CGG | + | 42982:11900-11919 | None:intergenic | 55.0% | |
| CTATCCCTCTACAACCACCC+CGG | - | 42982:11652-11671 | MS.gene30687:CDS | 55.0% | |
| GACGACGGAGAAGCCTAAAC+GGG | + | 42982:11899-11918 | None:intergenic | 55.0% | |
| GCCAAACCAGCCAAAGTAGG+AGG | + | 42982:12573-12592 | None:intergenic | 55.0% | |
| GCCGATGTTAGCGGTTGTAG+TGG | + | 42982:11866-11885 | None:intergenic | 55.0% | |
| GGTTCCAACTCCAGTTCCAC+TGG | - | 42982:12207-12226 | MS.gene30687:CDS | 55.0% | |
| GGTTTGGGGAATGTGGGAGA+GGG | + | 42982:11727-11746 | None:intergenic | 55.0% | |
| TGCTTGGAGACTTGTGGAGC+AGG | - | 42982:12605-12624 | MS.gene30687:CDS | 55.0% | |
| TGTGGGAGAGGGATGGAGAT+GGG | + | 42982:11716-11735 | None:intergenic | 55.0% | |
| ! | GCCTCCTACTTTGGCTGGTT+TGG | - | 42982:12569-12588 | MS.gene30687:CDS | 55.0% |
| ! | GGGTTTGGGTTTGGGGAATG+TGG | + | 42982:11734-11753 | None:intergenic | 55.0% |
| !! | TTCCACTGGTTCTGGCTGTC+CGG | - | 42982:12221-12240 | MS.gene30687:CDS | 55.0% |
| !! | TTGAGATTGGTGGTGGTGCC+GGG | + | 42982:11673-11692 | None:intergenic | 55.0% |
| !!! | TCCACCGGTTTTATCTCCCC+GGG | + | 42982:12154-12173 | None:intergenic | 55.0% |
| GGGTTTGGGGAATGTGGGAG+AGG | + | 42982:11728-11747 | None:intergenic | 60.0% | |
| TACAACGCGTGGGGTTTCGC+CGG | + | 42982:11809-11828 | None:intergenic | 60.0% | |
| TCCTGATCGATTGCCCAGCG+GGG | - | 42982:12250-12269 | MS.gene30687:CDS | 60.0% | |
| TGGGGAATGTGGGAGAGGGA+TGG | + | 42982:11723-11742 | None:intergenic | 60.0% | |
| TGGGGTTTCGCCGGAGAGTT+GGG | + | 42982:11800-11819 | None:intergenic | 60.0% | |
| ! | TAATCCGACGTCGGCGTCGA+TGG | + | 42982:11923-11942 | None:intergenic | 60.0% |
| !! | TGAGATTGGTGGTGGTGCCG+GGG | + | 42982:11672-11691 | None:intergenic | 60.0% |
| CGCCGGAGAGTTGGGGTTTG+CGG | + | 42982:11792-11811 | None:intergenic | 65.0% | |
| GCCCGGGGAGATAAAACCGG+TGG | - | 42982:12150-12169 | MS.gene30687:CDS | 65.0% | |
| GGGGTTTCGCCGGAGAGTTG+GGG | + | 42982:11799-11818 | None:intergenic | 65.0% | |
| GTCCGGACAGCCAGAACCAG+TGG | + | 42982:12226-12245 | None:intergenic | 65.0% | |
| GTGGGGTTTCGCCGGAGAGT+TGG | + | 42982:11801-11820 | None:intergenic | 65.0% | |
| TCCCCGCTGGGCAATCGATC+AGG | + | 42982:12254-12273 | None:intergenic | 65.0% | |
| ! | AACCCTGCATCGATCCCCGC+TGG | + | 42982:12267-12286 | None:intergenic | 65.0% |
| ! | ACCCTGCATCGATCCCCGCT+GGG | + | 42982:12266-12285 | None:intergenic | 65.0% |
| ! | GGTGCCGGGGTGGTTGTAGA+GGG | + | 42982:11659-11678 | None:intergenic | 65.0% |
| ! | TGGTGCCGGGGTGGTTGTAG+AGG | + | 42982:11660-11679 | None:intergenic | 65.0% |
| GCCCAGCGGGGATCGATGCA+GGG | - | 42982:12262-12281 | MS.gene30687:CDS | 70.0% | |
| GTCGCCATCGACGCCGACGT+CGG | - | 42982:11916-11935 | MS.gene30687:CDS | 70.0% | |
| TGCCCAGCGGGGATCGATGC+AGG | - | 42982:12261-12280 | MS.gene30687:CDS | 70.0% | |
| !! | GATTGGTGGTGGTGCCGGGG+TGG | + | 42982:11669-11688 | None:intergenic | 70.0% |
| !! | GTCGGCGTCGATGGCGACGA+CGG | + | 42982:11914-11933 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 42982 | gene | 11649 | 12695 | 11649 | ID=MS.gene30687 |
| 42982 | mRNA | 11649 | 12695 | 11649 | ID=MS.gene30687.t1;Parent=MS.gene30687 |
| 42982 | exon | 11649 | 12695 | 11649 | ID=MS.gene30687.t1.exon1;Parent=MS.gene30687.t1 |
| 42982 | CDS | 11649 | 12695 | 11649 | ID=cds.MS.gene30687.t1;Parent=MS.gene30687.t1 |
| Gene Sequence |
| Protein sequence |
