Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30708.t1 | XP_013452196.1 | 95.5 | 443 | 18 | 2 | 1 | 443 | 1 | 441 | 2.60E-221 | 778.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30708.t1 | O31634 | 29.9 | 388 | 246 | 6 | 78 | 443 | 2 | 385 | 1.0e-37 | 159.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30708.t1 | A0A072U8V4 | 95.5 | 443 | 18 | 2 | 1 | 443 | 1 | 441 | 1.9e-221 | 778.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059384 | MS.gene30708 | 0.808282 | 3.34E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30708.t1 | MTR_6g048050 | 95.485 | 443 | 18 | 2 | 1 | 443 | 1 | 441 | 0.0 | 775 |
| MS.gene30708.t1 | MTR_1g100120 | 60.049 | 408 | 155 | 2 | 54 | 456 | 42 | 446 | 2.11e-160 | 463 |
| MS.gene30708.t1 | MTR_1g100120 | 57.667 | 300 | 122 | 1 | 54 | 348 | 42 | 341 | 1.04e-104 | 318 |
| MS.gene30708.t1 | MTR_0054s0050 | 62.147 | 177 | 8 | 2 | 267 | 443 | 4 | 121 | 4.18e-44 | 152 |
| MS.gene30708.t1 | MTR_7g060910 | 61.272 | 173 | 8 | 2 | 270 | 442 | 1 | 114 | 5.07e-40 | 144 |
| MS.gene30708.t1 | MTR_8g036190 | 87.719 | 57 | 7 | 0 | 267 | 323 | 4 | 60 | 5.52e-26 | 102 |
| MS.gene30708.t1 | MTR_8g036200 | 44.348 | 115 | 17 | 2 | 153 | 267 | 16 | 83 | 8.39e-14 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30708.t1 | AT5G24000 | 63.054 | 406 | 139 | 2 | 49 | 443 | 27 | 432 | 1.57e-170 | 489 |
| MS.gene30708.t1 | AT5G52540 | 58.701 | 431 | 168 | 4 | 29 | 456 | 37 | 460 | 4.49e-157 | 456 |
Find 124 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTTCAGCATTGGTGCTTT+TGG | 0.137500 | 6.2:-42363814 | MS.gene30708:CDS |
| ATCCATCTTCTTATTGTTCT+TGG | 0.155822 | 6.2:-42355414 | MS.gene30708:CDS |
| CATATCTGATACAAGGAATT+TGG | 0.174879 | 6.2:+42354627 | None:intergenic |
| CTTCTTATTGTTCTTGGATT+AGG | 0.227467 | 6.2:-42355408 | MS.gene30708:CDS |
| AGGTCGGAGAAGACTAAAAT+TGG | 0.252433 | 6.2:-42363113 | MS.gene30708:CDS |
| CAGTATGACCAGCAGGTATA+AGG | 0.256942 | 6.2:+42355963 | None:intergenic |
| CAAATTCCTTGTATCAGATA+TGG | 0.265035 | 6.2:-42354626 | MS.gene30708:CDS |
| TTAGCATCAAATGCTAATAT+TGG | 0.269367 | 6.2:-42355351 | MS.gene30708:CDS |
| ACTATTCCATTGCTATTGTT+TGG | 0.277840 | 6.2:-42362972 | MS.gene30708:CDS |
| ATTGCAGTTGCTACTATAAT+TGG | 0.291922 | 6.2:-42362656 | MS.gene30708:intron |
| CTGATGATCATTGGGGTGTT+TGG | 0.308984 | 6.2:-42363843 | MS.gene30708:CDS |
| GGTGATAGGTACCTGGATAT+TGG | 0.324720 | 6.2:-42354425 | MS.gene30708:CDS |
| GAAAGTTGCAGCAGCTCTAA+TGG | 0.324983 | 6.2:-42362579 | MS.gene30708:CDS |
| GCTGCAACTTTCCAGTTATC+AGG | 0.326869 | 6.2:+42362590 | None:intergenic |
| GCATATGAAGTCTGAAAATC+AGG | 0.327161 | 6.2:-42356152 | MS.gene30708:CDS |
| GATCTCTTGGTCCTGATAAC+TGG | 0.334655 | 6.2:-42362601 | MS.gene30708:CDS |
| GCTCTAATGGGTAGCTATAT+TGG | 0.344060 | 6.2:-42362566 | MS.gene30708:CDS |
| CATATCTCCTGATGATCATT+GGG | 0.348308 | 6.2:-42363851 | MS.gene30708:CDS |
| CAACAAGTAGTTCGTTCAAC+TGG | 0.349880 | 6.2:-42362939 | MS.gene30708:CDS |
| GTTGTTCCTGGAATCCTTAC+CGG | 0.352710 | 6.2:-42355276 | MS.gene30708:CDS |
| ACATACTTCACAAAATTATA+CGG | 0.353945 | 6.2:-42356064 | MS.gene30708:CDS |
| TGGGAATCTTTAGTTGTTCC+TGG | 0.360735 | 6.2:-42355288 | MS.gene30708:CDS |
| TCATTGTCTGTTTCTCTCCC+TGG | 0.368240 | 6.2:+42354320 | None:intergenic |
| TGAATATACTTGGGGCAGTT+TGG | 0.369634 | 6.2:+42355460 | None:intergenic |
| GAATAAACCTGCCAAGTAAC+AGG | 0.375739 | 6.2:+42354362 | None:intergenic |
| ATACAAGGAGGAACTCTTCC+AGG | 0.383460 | 6.2:-42356040 | MS.gene30708:CDS |
| GTGGCTTTCTTACTTGGGTC+AGG | 0.387488 | 6.2:-42362906 | MS.gene30708:intron |
| GGACTTGCGGCAAGTAATCT+TGG | 0.394113 | 6.2:-42363053 | MS.gene30708:CDS |
| GTGATAGGTACCTGGATATT+GGG | 0.394697 | 6.2:-42354424 | MS.gene30708:CDS |
| TCATATCTCCTGATGATCAT+TGG | 0.399513 | 6.2:-42363852 | MS.gene30708:CDS |
| TAACAGGCTTCCTTGTACTA+AGG | 0.403565 | 6.2:+42354378 | None:intergenic |
| TCCTTTAATAGGAAGGTGAT+AGG | 0.404834 | 6.2:-42354439 | MS.gene30708:intron |
| AAAATGCCGGTAAGGATTCC+AGG | 0.407085 | 6.2:+42355270 | None:intergenic |
| CAGTGACAGGTGGGTGTTTG+AGG | 0.407502 | 6.2:+42364082 | None:intergenic |
| CAAACCAAGCGCCTCTGAAA+TGG | 0.410454 | 6.2:+42361936 | None:intergenic |
| GCTTTAGTGAGCACATTAGT+TGG | 0.416021 | 6.2:-42363074 | MS.gene30708:CDS |
| TGCTGGTCATACTGTTGCCT+TGG | 0.431355 | 6.2:-42355954 | MS.gene30708:CDS |
| AGTATGACCAGCAGGTATAA+GGG | 0.443311 | 6.2:+42355964 | None:intergenic |
| GCCATTCCACAAGCTGTTGT+AGG | 0.444869 | 6.2:+42355324 | None:intergenic |
| GTACAAGGAAGCCTGTTACT+TGG | 0.461015 | 6.2:-42354373 | MS.gene30708:CDS |
| AAGTATATTCATGTTTGCAC+TGG | 0.466227 | 6.2:-42355448 | MS.gene30708:CDS |
| GCTGGTGGTGCAGTCTCTGC+GGG | 0.471768 | 6.2:+42361823 | None:intergenic |
| AAGGAAGCCTGTTACTTGGC+AGG | 0.472859 | 6.2:-42354369 | MS.gene30708:CDS |
| AAAGTTGCAGCAGCTCTAAT+GGG | 0.474333 | 6.2:-42362578 | MS.gene30708:CDS |
| TGATCATCAGGAGATATGAT+AGG | 0.483007 | 6.2:+42363856 | None:intergenic |
| AATAGCAATGGAATAGTAAG+AGG | 0.484012 | 6.2:+42362978 | None:intergenic |
| ATAAGGGGACTAATCAGCTT+TGG | 0.486288 | 6.2:+42355980 | None:intergenic |
| GCAGTAGCTGTCTGTAACAC+AGG | 0.490849 | 6.2:+42356121 | None:intergenic |
| GAATGGCTAAAGCTAAAGGC+TGG | 0.495478 | 6.2:-42355308 | MS.gene30708:CDS |
| AGATATGGAAACAAGCTTGT+AGG | 0.496635 | 6.2:-42354611 | MS.gene30708:CDS |
| TGACATCAGGCCCAATATCC+AGG | 0.497672 | 6.2:+42354414 | None:intergenic |
| CAAACACCCCAATGATCATC+AGG | 0.499006 | 6.2:+42363844 | None:intergenic |
| TGTTGCCTTGGTCTTAATGC+AGG | 0.499586 | 6.2:-42355942 | MS.gene30708:intron |
| CCATTCCACAAGCTGTTGTA+GGG | 0.501911 | 6.2:+42355325 | None:intergenic |
| ATTAGTCCCCTTATACCTGC+TGG | 0.502272 | 6.2:-42355971 | MS.gene30708:CDS |
| AGTGCAAACATGAATATACT+TGG | 0.504219 | 6.2:+42355450 | None:intergenic |
| ATCCAAGAACAATAAGAAGA+TGG | 0.504284 | 6.2:+42355412 | None:intergenic |
| ACCGGCATATTCTATTGTCT+TGG | 0.505721 | 6.2:-42363012 | MS.gene30708:CDS |
| TGGCGTGATGGGATTGGTTG+TGG | 0.507610 | 6.2:+42364048 | None:intergenic |
| CGGAATGATACGTGGCGTGA+TGG | 0.509564 | 6.2:+42364036 | None:intergenic |
| CGGACATGAATTGAGGATGT+TGG | 0.510088 | 6.2:+42363987 | None:intergenic |
| TGGTCTGCTATCTTCAGCAT+TGG | 0.510382 | 6.2:-42363823 | MS.gene30708:CDS |
| ATAGTACCTATGAGATCTCT+TGG | 0.510450 | 6.2:-42362614 | MS.gene30708:CDS |
| CCTACATCAGTGATGACATC+AGG | 0.512562 | 6.2:+42354401 | None:intergenic |
| TGAGGTTGTAGTAAGAGGTT+CGG | 0.514126 | 6.2:+42363967 | None:intergenic |
| GCATCAAATGCTAATATTGG+AGG | 0.516785 | 6.2:-42355348 | MS.gene30708:CDS |
| ACGATGATTGCAGTTACTCC+TGG | 0.520577 | 6.2:+42356022 | None:intergenic |
| CTGATGTAGGCCTTAGTACA+AGG | 0.520978 | 6.2:-42354388 | MS.gene30708:CDS |
| GTGCAAACATGAATATACTT+GGG | 0.524524 | 6.2:+42355451 | None:intergenic |
| CCTGATGTCATCACTGATGT+AGG | 0.526562 | 6.2:-42354401 | MS.gene30708:CDS |
| CGTGATGGGATTGGTTGTGG+TGG | 0.530923 | 6.2:+42364051 | None:intergenic |
| GGTCGGAGAAGACTAAAATT+GGG | 0.531713 | 6.2:-42363112 | MS.gene30708:CDS |
| AGAAGAAAGAAAGTGGGACC+AGG | 0.532675 | 6.2:-42354338 | MS.gene30708:CDS |
| GATACGTGGCGTGATGGGAT+TGG | 0.533559 | 6.2:+42364042 | None:intergenic |
| TTGTTTCCATATCTGATACA+AGG | 0.539659 | 6.2:+42354620 | None:intergenic |
| TGTGGAATGGCTAAAGCTAA+AGG | 0.546930 | 6.2:-42355312 | MS.gene30708:CDS |
| GTTGCCATTTCAGAGGCGCT+TGG | 0.549654 | 6.2:-42361940 | MS.gene30708:CDS |
| GGAATGATACGTGGCGTGAT+GGG | 0.553809 | 6.2:+42364037 | None:intergenic |
| CCCTACAACAGCTTGTGGAA+TGG | 0.557209 | 6.2:-42355325 | MS.gene30708:CDS |
| GAGACTGCACCACCAGCCAC+TGG | 0.558726 | 6.2:-42361817 | MS.gene30708:intron |
| TTTGTGGTGGTTGGAGCTAG+TGG | 0.561107 | 6.2:-42355501 | MS.gene30708:CDS |
| TCAGGACCAAGAGATCTCAT+AGG | 0.565976 | 6.2:+42362608 | None:intergenic |
| TGTGTTGAGGTTGTAGTAAG+AGG | 0.573439 | 6.2:+42363962 | None:intergenic |
| AATTCTTCCACATGATGCAC+CGG | 0.574454 | 6.2:-42363030 | MS.gene30708:CDS |
| TTTGCTCCAAACAATAGCAA+TGG | 0.576769 | 6.2:+42362966 | None:intergenic |
| AAGGCAACAGTATGACCAGC+AGG | 0.578651 | 6.2:+42355956 | None:intergenic |
| GGCTGGTGGTGCAGTCTCTG+CGG | 0.579584 | 6.2:+42361822 | None:intergenic |
| AGAGGTTCGGACATGAATTG+AGG | 0.583063 | 6.2:+42363980 | None:intergenic |
| GGGTGTTTGAGGAGAGACAT+TGG | 0.585033 | 6.2:+42364093 | None:intergenic |
| GAATATGCCGGTGCATCATG+TGG | 0.585225 | 6.2:+42363023 | None:intergenic |
| GAAGACTAAAATTGGGAGCA+TGG | 0.585584 | 6.2:-42363105 | MS.gene30708:CDS |
| ACATGAATTGAGGATGTTGG+AGG | 0.585952 | 6.2:+42363990 | None:intergenic |
| AATCAGCTTTGGTGCAAGAG+TGG | 0.586932 | 6.2:+42355991 | None:intergenic |
| ATTGTTGTAGTGGTGTGTTG+AGG | 0.587057 | 6.2:+42363949 | None:intergenic |
| GTATGACCAGCAGGTATAAG+GGG | 0.591828 | 6.2:+42355965 | None:intergenic |
| GAAGAAAGAAAGTGGGACCA+GGG | 0.591981 | 6.2:-42354337 | MS.gene30708:CDS |
| TTATTCTAGAAGAAAGAAAG+TGG | 0.592745 | 6.2:-42354345 | MS.gene30708:CDS |
| AATTACCTCGTATCTGTCAA+AGG | 0.593217 | 6.2:-42363929 | MS.gene30708:CDS |
| ATACGAGGTAATTGTTGTAG+TGG | 0.597771 | 6.2:+42363939 | None:intergenic |
| GAGAGAAACAGACAATGAAG+AGG | 0.599425 | 6.2:-42354315 | MS.gene30708:CDS |
| GGAGGCCCTACAACAGCTTG+TGG | 0.599549 | 6.2:-42355330 | MS.gene30708:CDS |
| TACTATAATTGGTACTCTAG+TGG | 0.601056 | 6.2:-42362645 | MS.gene30708:CDS |
| TAATTATGTTGCCATTTCAG+AGG | 0.603851 | 6.2:-42361947 | MS.gene30708:CDS |
| TCCAAGACAATAGAATATGC+CGG | 0.604479 | 6.2:+42363011 | None:intergenic |
| CTAATGGGTAGCTATATTGG+TGG | 0.610720 | 6.2:-42362563 | MS.gene30708:CDS |
| ACAAAATTATACGGTATACA+AGG | 0.612153 | 6.2:-42356055 | MS.gene30708:CDS |
| ATAGGAAGGTGATAGGTACC+TGG | 0.615311 | 6.2:-42354432 | MS.gene30708:intron |
| ATCTAGTACAGAGAGAATGT+CGG | 0.620043 | 6.2:+42364016 | None:intergenic |
| CATATGAAGTCTGAAAATCA+GGG | 0.621061 | 6.2:-42356151 | MS.gene30708:CDS |
| GAGCACATTAGTTGGACTTG+CGG | 0.623052 | 6.2:-42363066 | MS.gene30708:CDS |
| TATTCTAGAAGAAAGAAAGT+GGG | 0.631480 | 6.2:-42354344 | MS.gene30708:CDS |
| GAGAGAGAACAGTGACAGGT+GGG | 0.631941 | 6.2:+42364073 | None:intergenic |
| TCTGATACAAGGAATTTGGA+AGG | 0.633076 | 6.2:+42354631 | None:intergenic |
| TGTTACCTGCATTAAGACCA+AGG | 0.633531 | 6.2:+42355937 | None:intergenic |
| AATGGCTAAAGCTAAAGGCT+GGG | 0.641949 | 6.2:-42355307 | MS.gene30708:CDS |
| TGCAAACATGAATATACTTG+GGG | 0.655619 | 6.2:+42355452 | None:intergenic |
| GGTGGAGAGAGAACAGTGAC+AGG | 0.664373 | 6.2:+42364069 | None:intergenic |
| GAGAATGTCGGAATGATACG+TGG | 0.677053 | 6.2:+42364028 | None:intergenic |
| ATATCTCCTGATGATCATTG+GGG | 0.680137 | 6.2:-42363850 | MS.gene30708:CDS |
| GGAGAGAGAACAGTGACAGG+TGG | 0.683656 | 6.2:+42364072 | None:intergenic |
| AAAATAGTACCAGTGGCTGG+TGG | 0.703775 | 6.2:+42361808 | None:intergenic |
| AAATTATACGGTATACAAGG+AGG | 0.729505 | 6.2:-42356052 | MS.gene30708:CDS |
| ACAACACTTCGAGTTAAACG+AGG | 0.729919 | 6.2:+42363904 | None:intergenic |
| AGGTTCCTTTGACAGATACG+AGG | 0.742128 | 6.2:+42363924 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 42354310 | 42364112 | 42354310 | ID=MS.gene30708 |
| chr6.2 | mRNA | 42354310 | 42364112 | 42354310 | ID=MS.gene30708.t1;Parent=MS.gene30708 |
| chr6.2 | exon | 42363808 | 42364112 | 42363808 | ID=MS.gene30708.t1.exon1;Parent=MS.gene30708.t1 |
| chr6.2 | CDS | 42363808 | 42364112 | 42363808 | ID=cds.MS.gene30708.t1;Parent=MS.gene30708.t1 |
| chr6.2 | exon | 42362907 | 42363133 | 42362907 | ID=MS.gene30708.t1.exon2;Parent=MS.gene30708.t1 |
| chr6.2 | CDS | 42362907 | 42363133 | 42362907 | ID=cds.MS.gene30708.t1;Parent=MS.gene30708.t1 |
| chr6.2 | exon | 42362561 | 42362671 | 42362561 | ID=MS.gene30708.t1.exon3;Parent=MS.gene30708.t1 |
| chr6.2 | CDS | 42362561 | 42362671 | 42362561 | ID=cds.MS.gene30708.t1;Parent=MS.gene30708.t1 |
| chr6.2 | exon | 42361818 | 42361973 | 42361818 | ID=MS.gene30708.t1.exon4;Parent=MS.gene30708.t1 |
| chr6.2 | CDS | 42361818 | 42361973 | 42361818 | ID=cds.MS.gene30708.t1;Parent=MS.gene30708.t1 |
| chr6.2 | exon | 42355943 | 42356184 | 42355943 | ID=MS.gene30708.t1.exon5;Parent=MS.gene30708.t1 |
| chr6.2 | CDS | 42355943 | 42356184 | 42355943 | ID=cds.MS.gene30708.t1;Parent=MS.gene30708.t1 |
| chr6.2 | exon | 42355244 | 42355529 | 42355244 | ID=MS.gene30708.t1.exon6;Parent=MS.gene30708.t1 |
| chr6.2 | CDS | 42355244 | 42355529 | 42355244 | ID=cds.MS.gene30708.t1;Parent=MS.gene30708.t1 |
| chr6.2 | exon | 42354571 | 42354668 | 42354571 | ID=MS.gene30708.t1.exon7;Parent=MS.gene30708.t1 |
| chr6.2 | CDS | 42354571 | 42354668 | 42354571 | ID=cds.MS.gene30708.t1;Parent=MS.gene30708.t1 |
| chr6.2 | exon | 42354310 | 42354450 | 42354310 | ID=MS.gene30708.t1.exon8;Parent=MS.gene30708.t1 |
| chr6.2 | CDS | 42354310 | 42354450 | 42354310 | ID=cds.MS.gene30708.t1;Parent=MS.gene30708.t1 |
| Gene Sequence |
| Protein sequence |
