Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31036.t1 | XP_003602491.1 | 98.8 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 4.70E-40 | 173.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31036.t1 | G7JAB0 | 98.8 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 3.4e-40 | 173.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049358 | MS.gene31036 | 0.989234 | 2.96E-177 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31036.t1 | MTR_3g094970 | 98.824 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 1.29e-59 | 178 |
| MS.gene31036.t1 | MTR_3g094970 | 100.000 | 55 | 0 | 0 | 31 | 85 | 1 | 55 | 7.62e-36 | 117 |
| MS.gene31036.t1 | MTR_8g091930 | 79.310 | 58 | 12 | 0 | 1 | 58 | 3 | 60 | 1.35e-29 | 102 |
| MS.gene31036.t1 | MTR_8g091930 | 79.310 | 58 | 12 | 0 | 1 | 58 | 3 | 60 | 1.45e-29 | 102 |
| MS.gene31036.t1 | MTR_8g091930 | 77.778 | 54 | 12 | 0 | 5 | 58 | 6 | 59 | 2.92e-27 | 96.7 |
| MS.gene31036.t1 | MTR_8g091930 | 77.778 | 54 | 12 | 0 | 5 | 58 | 6 | 59 | 3.22e-27 | 96.7 |
| MS.gene31036.t1 | MTR_2g036020 | 63.333 | 60 | 16 | 1 | 1 | 60 | 1 | 54 | 1.09e-18 | 74.3 |
| MS.gene31036.t1 | MTR_2g035990 | 60.317 | 63 | 19 | 1 | 1 | 63 | 1 | 57 | 1.82e-18 | 73.9 |
| MS.gene31036.t1 | MTR_2g035950 | 58.333 | 60 | 19 | 1 | 1 | 60 | 1 | 54 | 2.49e-16 | 68.6 |
| MS.gene31036.t1 | MTR_3g094890 | 48.485 | 66 | 30 | 1 | 6 | 71 | 2 | 63 | 3.81e-12 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31036.t1 | AT5G09960 | 77.966 | 59 | 11 | 1 | 5 | 61 | 6 | 64 | 5.98e-28 | 98.6 |
| MS.gene31036.t1 | AT5G64850 | 66.667 | 75 | 24 | 1 | 1 | 74 | 1 | 75 | 7.54e-28 | 98.6 |
| MS.gene31036.t1 | AT5G09960 | 77.193 | 57 | 11 | 1 | 7 | 61 | 6 | 62 | 1.43e-26 | 95.1 |
| MS.gene31036.t1 | AT5G19473 | 42.045 | 88 | 44 | 3 | 1 | 86 | 1 | 83 | 2.37e-15 | 66.2 |
| MS.gene31036.t1 | AT5G19473 | 46.575 | 73 | 33 | 2 | 7 | 77 | 41 | 109 | 1.06e-14 | 65.5 |
| MS.gene31036.t1 | AT5G09960 | 77.778 | 36 | 8 | 0 | 26 | 61 | 1 | 36 | 2.80e-14 | 63.2 |
Find 34 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATCTTACCACTGCAGAAT+TGG | 0.341653 | 3.3:+78802193 | None:intergenic |
| ATACTCTATCATTGGATGAT+AGG | 0.354161 | 3.3:-78802058 | MS.gene31036:CDS |
| TTTGTGGTAGTGAGGATGCT+GGG | 0.363051 | 3.3:+78802217 | None:intergenic |
| GGATCAGAAGGGCCCATTGC+CGG | 0.392403 | 3.3:-78802375 | MS.gene31036:CDS |
| TGGCTATCGGTCCCACAGTT+TGG | 0.419649 | 3.3:-78802404 | MS.gene31036:CDS |
| AATTCCTCATACTCTATCAT+TGG | 0.429040 | 3.3:-78802066 | MS.gene31036:CDS |
| TTACTATAGTAGTTGAATAG+TGG | 0.448174 | 3.3:-78802107 | MS.gene31036:intron |
| TGGCAATGAGGAGGAGTTTG+CGG | 0.468439 | 3.3:-78802279 | MS.gene31036:CDS |
| AAGACTTGCTCTTGAAGCAT+TGG | 0.469288 | 3.3:+78802301 | None:intergenic |
| GGTCCCACAGTTTGGCGAAT+GGG | 0.471256 | 3.3:-78802396 | MS.gene31036:CDS |
| GAATAGTGGCCCGAGTTCAC+AGG | 0.477765 | 3.3:-78802093 | MS.gene31036:CDS |
| ATGCTGGGGTTCGCTGTGAT+CGG | 0.500648 | 3.3:+78802232 | None:intergenic |
| TCATCCAATGATAGAGTATG+AGG | 0.501733 | 3.3:+78802062 | None:intergenic |
| CCCATTGCCGGACTATTCCA+TGG | 0.515219 | 3.3:-78802363 | MS.gene31036:CDS |
| TCCATGGAATAGTCCGGCAA+TGG | 0.528219 | 3.3:+78802362 | None:intergenic |
| CCATGGAATAGTCCGGCAAT+GGG | 0.531041 | 3.3:+78802363 | None:intergenic |
| CGGTCCCACAGTTTGGCGAA+TGG | 0.553714 | 3.3:-78802397 | MS.gene31036:CDS |
| GTTTGGCGAATGGGATCAGA+AGG | 0.556862 | 3.3:-78802387 | MS.gene31036:CDS |
| GCCTTTACGTTCTTCTGAGT+AGG | 0.564283 | 3.3:+78802254 | None:intergenic |
| ACTTATGGAGCAAAATAAAG+AGG | 0.574874 | 3.3:-78803096 | None:intergenic |
| TTGTGGTAGTGAGGATGCTG+GGG | 0.577503 | 3.3:+78802218 | None:intergenic |
| AACTGTGGGACCGATAGCCA+AGG | 0.578221 | 3.3:+78802407 | None:intergenic |
| AATAGTGGCCCGAGTTCACA+GGG | 0.588264 | 3.3:-78802092 | MS.gene31036:CDS |
| TGATCCCATTCGCCAAACTG+TGG | 0.590185 | 3.3:+78802392 | None:intergenic |
| GATCCCATTCGCCAAACTGT+GGG | 0.591973 | 3.3:+78802393 | None:intergenic |
| AATGCTTCAAGAGCAAGTCT+TGG | 0.596021 | 3.3:-78802299 | MS.gene31036:CDS |
| AAGAGCAAGTCTTGGCAATG+AGG | 0.602463 | 3.3:-78802291 | MS.gene31036:CDS |
| ACTGTGGGACCGATAGCCAA+GGG | 0.608973 | 3.3:+78802408 | None:intergenic |
| AGCAAGTCTTGGCAATGAGG+AGG | 0.615986 | 3.3:-78802288 | MS.gene31036:CDS |
| TCCTACTCAGAAGAACGTAA+AGG | 0.619222 | 3.3:-78802255 | MS.gene31036:CDS |
| CCACAAAACCAATTCTGCAG+TGG | 0.661784 | 3.3:-78802201 | MS.gene31036:intron |
| CTGTGGGACCGATAGCCAAG+GGG | 0.713527 | 3.3:+78802409 | None:intergenic |
| TTTGGCGAATGGGATCAGAA+GGG | 0.720121 | 3.3:-78802386 | MS.gene31036:CDS |
| ATAGTGGCCCGAGTTCACAG+GGG | 0.833581 | 3.3:-78802091 | MS.gene31036:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGTTACAACAAATAAAATT+AGG | + | chr3.3:78802351-78802370 | None:intergenic | 15.0% |
| !! | GAATTACTACATAAGTTTAT+AGG | + | chr3.3:78802196-78802215 | None:intergenic | 20.0% |
| ! | AGAATACTGATCATCATAAT+AGG | + | chr3.3:78802672-78802691 | None:intergenic | 25.0% |
| ! | CGAGATTAGATTGATAAATT+AGG | + | chr3.3:78802257-78802276 | None:intergenic | 25.0% |
| ! | TATCTGTATAGCTCAATAAT+GGG | - | chr3.3:78802986-78803005 | MS.gene31036:intron | 25.0% |
| ! | TTACTATAGTAGTTGAATAG+TGG | - | chr3.3:78803025-78803044 | MS.gene31036:intron | 25.0% |
| ! | TTATCTGTATAGCTCAATAA+TGG | - | chr3.3:78802985-78803004 | MS.gene31036:intron | 25.0% |
| !! | ATAATTCATGTTTCTGTGTT+AGG | - | chr3.3:78802146-78802165 | MS.gene31036:intron | 25.0% |
| !!! | CATGGATTTTTCGAAAATTA+GGG | - | chr3.3:78802787-78802806 | MS.gene31036:intron | 25.0% |
| AATTCCTCATACTCTATCAT+TGG | - | chr3.3:78803066-78803085 | MS.gene31036:intron | 30.0% | |
| ACATATTTGTTTCCTTAACC+AGG | + | chr3.3:78802502-78802521 | None:intergenic | 30.0% | |
| ATACTCTATCATTGGATGAT+AGG | - | chr3.3:78803074-78803093 | MS.gene31036:intron | 30.0% | |
| GAAAAATGTTAACAAGTGTC+CGG | - | chr3.3:78802473-78802492 | MS.gene31036:intron | 30.0% | |
| TTGCAGAATAATCACTTCAT+AGG | + | chr3.3:78802317-78802336 | None:intergenic | 30.0% | |
| ! | CCTAATTTTCGAAAAATCCA+TGG | + | chr3.3:78802789-78802808 | None:intergenic | 30.0% |
| !! | CCATGGATTTTTCGAAAATT+AGG | - | chr3.3:78802786-78802805 | MS.gene31036:intron | 30.0% |
| ATCTATACGCTTTCGTTTCT+TGG | - | chr3.3:78802086-78802105 | MS.gene31036:CDS | 35.0% | |
| GAAAAATCCATGGAATAGTC+CGG | + | chr3.3:78802779-78802798 | None:intergenic | 35.0% | |
| TCATCCAATGATAGAGTATG+AGG | + | chr3.3:78803073-78803092 | None:intergenic | 35.0% | |
| !! | TCAATAATGGGTTGTCAACA+TGG | - | chr3.3:78802998-78803017 | MS.gene31036:intron | 35.0% |
| AAATTCGCCTCTCCTTGTAA+CGG | - | chr3.3:78802411-78802430 | MS.gene31036:CDS | 40.0% | |
| AATGCTTCAAGAGCAAGTCT+TGG | - | chr3.3:78802833-78802852 | MS.gene31036:intron | 40.0% | |
| GAATCTTACCACTGCAGAAT+TGG | + | chr3.3:78802942-78802961 | None:intergenic | 40.0% | |
| TCCTACTCAGAAGAACGTAA+AGG | - | chr3.3:78802877-78802896 | MS.gene31036:intron | 40.0% | |
| ! | AAGACTTGCTCTTGAAGCAT+TGG | + | chr3.3:78802834-78802853 | None:intergenic | 40.0% |
| !!! | GAATTGGTTTTGTGGTAGTG+AGG | + | chr3.3:78802926-78802945 | None:intergenic | 40.0% |
| AAGAGCAAGTCTTGGCAATG+AGG | - | chr3.3:78802841-78802860 | MS.gene31036:intron | 45.0% | |
| AGAAGAACCGTTACAAGGAG+AGG | + | chr3.3:78802421-78802440 | None:intergenic | 45.0% | |
| AGACCAGAAGAACCGTTACA+AGG | + | chr3.3:78802426-78802445 | None:intergenic | 45.0% | |
| AGGAATCGAAGCTTGCGATT+CGG | + | chr3.3:78802176-78802195 | None:intergenic | 45.0% | |
| CCACAAAACCAATTCTGCAG+TGG | - | chr3.3:78802931-78802950 | MS.gene31036:intron | 45.0% | |
| GCCTTTACGTTCTTCTGAGT+AGG | + | chr3.3:78802881-78802900 | None:intergenic | 45.0% | |
| TCTCCTTGTAACGGTTCTTC+TGG | - | chr3.3:78802420-78802439 | MS.gene31036:intron | 45.0% | |
| TGTAACGGTTCTTCTGGTCT+AGG | - | chr3.3:78802426-78802445 | MS.gene31036:intron | 45.0% | |
| TGTTTCCTTAACCAGGCAAC+CGG | + | chr3.3:78802495-78802514 | None:intergenic | 45.0% | |
| TTTGGCGAATGGGATCAGAA+GGG | - | chr3.3:78802746-78802765 | MS.gene31036:intron | 45.0% | |
| ! | GAATTTTCCCCTGTGAACTC+GGG | + | chr3.3:78803051-78803070 | None:intergenic | 45.0% |
| ! | GGAATTTTCCCCTGTGAACT+CGG | + | chr3.3:78803052-78803071 | None:intergenic | 45.0% |
| ! | TTTGTGGTAGTGAGGATGCT+GGG | + | chr3.3:78802918-78802937 | None:intergenic | 45.0% |
| !! | TTTTGTGGTAGTGAGGATGC+TGG | + | chr3.3:78802919-78802938 | None:intergenic | 45.0% |
| !!! | CCACTGCAGAATTGGTTTTG+TGG | + | chr3.3:78802934-78802953 | None:intergenic | 45.0% |
| AATAGTGGCCCGAGTTCACA+GGG | - | chr3.3:78803040-78803059 | MS.gene31036:intron | 50.0% | |
| AGCAAGTCTTGGCAATGAGG+AGG | - | chr3.3:78802844-78802863 | MS.gene31036:intron | 50.0% | |
| CAATGCAGAAAAACGCCCCT+TGG | - | chr3.3:78802708-78802727 | MS.gene31036:intron | 50.0% | |
| CCATGGAATAGTCCGGCAAT+GGG | + | chr3.3:78802772-78802791 | None:intergenic | 50.0% | |
| GAAAAACGCCCCTTGGCTAT+CGG | - | chr3.3:78802715-78802734 | MS.gene31036:intron | 50.0% | |
| GATAAGAGTGAGCGAGAACG+AGG | + | chr3.3:78802069-78802088 | None:intergenic | 50.0% | |
| GATCCCATTCGCCAAACTGT+GGG | + | chr3.3:78802742-78802761 | None:intergenic | 50.0% | |
| GTTTGGCGAATGGGATCAGA+AGG | - | chr3.3:78802745-78802764 | MS.gene31036:intron | 50.0% | |
| TCCATGGAATAGTCCGGCAA+TGG | + | chr3.3:78802773-78802792 | None:intergenic | 50.0% | |
| TGATCCCATTCGCCAAACTG+TGG | + | chr3.3:78802743-78802762 | None:intergenic | 50.0% | |
| TGGCAATGAGGAGGAGTTTG+CGG | - | chr3.3:78802853-78802872 | MS.gene31036:intron | 50.0% | |
| TTAACAAGTGTCCGGTTGCC+TGG | - | chr3.3:78802481-78802500 | MS.gene31036:intron | 50.0% | |
| ! | TTGTGGTAGTGAGGATGCTG+GGG | + | chr3.3:78802917-78802936 | None:intergenic | 50.0% |
| AACTGTGGGACCGATAGCCA+AGG | + | chr3.3:78802728-78802747 | None:intergenic | 55.0% | |
| ACTGTGGGACCGATAGCCAA+GGG | + | chr3.3:78802727-78802746 | None:intergenic | 55.0% | |
| AGTGTCCGGTTGCCTGGTTA+AGG | - | chr3.3:78802487-78802506 | MS.gene31036:intron | 55.0% | |
| ATAGTGGCCCGAGTTCACAG+GGG | - | chr3.3:78803041-78803060 | MS.gene31036:intron | 55.0% | |
| ATGCTGGGGTTCGCTGTGAT+CGG | + | chr3.3:78802903-78802922 | None:intergenic | 55.0% | |
| CCCATTGCCGGACTATTCCA+TGG | - | chr3.3:78802769-78802788 | MS.gene31036:intron | 55.0% | |
| GAATAGTGGCCCGAGTTCAC+AGG | - | chr3.3:78803039-78803058 | MS.gene31036:intron | 55.0% | |
| GGTCCCACAGTTTGGCGAAT+GGG | - | chr3.3:78802736-78802755 | MS.gene31036:intron | 55.0% | |
| TGGCTATCGGTCCCACAGTT+TGG | - | chr3.3:78802728-78802747 | MS.gene31036:intron | 55.0% | |
| CGGTCCCACAGTTTGGCGAA+TGG | - | chr3.3:78802735-78802754 | MS.gene31036:intron | 60.0% | |
| CTGTGGGACCGATAGCCAAG+GGG | + | chr3.3:78802726-78802745 | None:intergenic | 60.0% | |
| GGATCAGAAGGGCCCATTGC+CGG | - | chr3.3:78802757-78802776 | MS.gene31036:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 78802040 | 78803114 | 78802040 | ID=MS.gene31036 |
| chr3.3 | mRNA | 78802040 | 78803114 | 78802040 | ID=MS.gene31036.t1;Parent=MS.gene31036 |
| chr3.3 | exon | 78803097 | 78803114 | 78803097 | ID=MS.gene31036.t1.exon1;Parent=MS.gene31036.t1 |
| chr3.3 | CDS | 78803097 | 78803114 | 78803097 | ID=cds.MS.gene31036.t1;Parent=MS.gene31036.t1 |
| chr3.3 | exon | 78802202 | 78802438 | 78802202 | ID=MS.gene31036.t1.exon2;Parent=MS.gene31036.t1 |
| chr3.3 | CDS | 78802202 | 78802438 | 78802202 | ID=cds.MS.gene31036.t1;Parent=MS.gene31036.t1 |
| chr3.3 | exon | 78802040 | 78802117 | 78802040 | ID=MS.gene31036.t1.exon3;Parent=MS.gene31036.t1 |
| chr3.3 | CDS | 78802040 | 78802117 | 78802040 | ID=cds.MS.gene31036.t1;Parent=MS.gene31036.t1 |
| Gene Sequence |
| Protein sequence |
