Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049358.t1 | XP_003602491.1 | 98.8 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 4.70E-40 | 173.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049358.t1 | G7JAB0 | 98.8 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 3.4e-40 | 173.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049358 | MS.gene06477 | 0.893746 | 4.72E-75 | -1.69E-46 |
| MS.gene049358 | MS.gene31036 | 0.989234 | 2.96E-177 | -1.69E-46 |
| MS.gene049358 | MS.gene44704 | 0.801971 | 7.00E-49 | -1.69E-46 |
| MS.gene049358 | MS.gene53820 | 0.828068 | 1.10E-54 | -1.69E-46 |
| MS.gene049358 | MS.gene94551 | 0.81637 | 5.73E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049358.t1 | MTR_3g094970 | 98.824 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 1.29e-59 | 178 |
| MS.gene049358.t1 | MTR_3g094970 | 100.000 | 55 | 0 | 0 | 31 | 85 | 1 | 55 | 7.62e-36 | 117 |
| MS.gene049358.t1 | MTR_8g091930 | 79.310 | 58 | 12 | 0 | 1 | 58 | 3 | 60 | 1.35e-29 | 102 |
| MS.gene049358.t1 | MTR_8g091930 | 79.310 | 58 | 12 | 0 | 1 | 58 | 3 | 60 | 1.45e-29 | 102 |
| MS.gene049358.t1 | MTR_8g091930 | 77.778 | 54 | 12 | 0 | 5 | 58 | 6 | 59 | 2.92e-27 | 96.7 |
| MS.gene049358.t1 | MTR_8g091930 | 77.778 | 54 | 12 | 0 | 5 | 58 | 6 | 59 | 3.22e-27 | 96.7 |
| MS.gene049358.t1 | MTR_2g036020 | 63.333 | 60 | 16 | 1 | 1 | 60 | 1 | 54 | 1.09e-18 | 74.3 |
| MS.gene049358.t1 | MTR_2g035990 | 60.317 | 63 | 19 | 1 | 1 | 63 | 1 | 57 | 1.82e-18 | 73.9 |
| MS.gene049358.t1 | MTR_2g035950 | 58.333 | 60 | 19 | 1 | 1 | 60 | 1 | 54 | 2.49e-16 | 68.6 |
| MS.gene049358.t1 | MTR_3g094890 | 48.485 | 66 | 30 | 1 | 6 | 71 | 2 | 63 | 3.81e-12 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049358.t1 | AT5G09960 | 77.966 | 59 | 11 | 1 | 5 | 61 | 6 | 64 | 5.98e-28 | 98.6 |
| MS.gene049358.t1 | AT5G64850 | 66.667 | 75 | 24 | 1 | 1 | 74 | 1 | 75 | 7.54e-28 | 98.6 |
| MS.gene049358.t1 | AT5G09960 | 77.193 | 57 | 11 | 1 | 7 | 61 | 6 | 62 | 1.43e-26 | 95.1 |
| MS.gene049358.t1 | AT5G19473 | 42.045 | 88 | 44 | 3 | 1 | 86 | 1 | 83 | 2.37e-15 | 66.2 |
| MS.gene049358.t1 | AT5G19473 | 46.575 | 73 | 33 | 2 | 7 | 77 | 41 | 109 | 1.06e-14 | 65.5 |
| MS.gene049358.t1 | AT5G09960 | 77.778 | 36 | 8 | 0 | 26 | 61 | 1 | 36 | 2.80e-14 | 63.2 |
Find 34 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATCTTACCACTGCAGAAT+TGG | 0.341653 | 3.1:+75739299 | None:intergenic |
| ATACTCTATCATTGGATGAT+AGG | 0.354161 | 3.1:-75739164 | MS.gene049358:CDS |
| TTTGTGGTAGTGAGGATGCT+GGG | 0.363051 | 3.1:+75739323 | None:intergenic |
| GGATCAGAAGGGCCCATTGC+CGG | 0.392403 | 3.1:-75739481 | MS.gene049358:CDS |
| TGGCTATCGGTCCCACAGTT+TGG | 0.419649 | 3.1:-75739510 | MS.gene049358:CDS |
| AATTCCTCATACTCTATCAT+TGG | 0.429040 | 3.1:-75739172 | MS.gene049358:CDS |
| TGGCAATGAGGAGGAGTTTG+CGG | 0.468439 | 3.1:-75739385 | MS.gene049358:CDS |
| AAGACTTGCTCTTGAAGCAT+TGG | 0.469288 | 3.1:+75739407 | None:intergenic |
| GGTCCCACAGTTTGGCGAAT+GGG | 0.471256 | 3.1:-75739502 | MS.gene049358:CDS |
| GAACAGTGGCCCGAGTTCAC+AGG | 0.474489 | 3.1:-75739199 | MS.gene049358:CDS |
| ATGCTGGGGTTCGCTGTGAT+CGG | 0.500648 | 3.1:+75739338 | None:intergenic |
| TCATCCAATGATAGAGTATG+AGG | 0.501733 | 3.1:+75739168 | None:intergenic |
| CCCATTGCCGGACTATTCCA+TGG | 0.515219 | 3.1:-75739469 | MS.gene049358:CDS |
| TCCATGGAATAGTCCGGCAA+TGG | 0.528219 | 3.1:+75739468 | None:intergenic |
| CCATGGAATAGTCCGGCAAT+GGG | 0.531041 | 3.1:+75739469 | None:intergenic |
| AACAGTGGCCCGAGTTCACA+GGG | 0.553337 | 3.1:-75739198 | MS.gene049358:CDS |
| CGGTCCCACAGTTTGGCGAA+TGG | 0.553714 | 3.1:-75739503 | MS.gene049358:CDS |
| GTTTGGCGAATGGGATCAGA+AGG | 0.556862 | 3.1:-75739493 | MS.gene049358:CDS |
| GCCTTTACGTTCTTCTGAGT+AGG | 0.564283 | 3.1:+75739360 | None:intergenic |
| ACTTATGGAGCAAAATAAAG+AGG | 0.574874 | 3.1:-75740183 | None:intergenic |
| TTGTGGTAGTGAGGATGCTG+GGG | 0.577503 | 3.1:+75739324 | None:intergenic |
| AACTGTGGGACCGATAGCCA+AGG | 0.578221 | 3.1:+75739513 | None:intergenic |
| TGATCCCATTCGCCAAACTG+TGG | 0.590185 | 3.1:+75739498 | None:intergenic |
| GATCCCATTCGCCAAACTGT+GGG | 0.591973 | 3.1:+75739499 | None:intergenic |
| AATGCTTCAAGAGCAAGTCT+TGG | 0.596021 | 3.1:-75739405 | MS.gene049358:CDS |
| AAGAGCAAGTCTTGGCAATG+AGG | 0.602463 | 3.1:-75739397 | MS.gene049358:CDS |
| ACTGTGGGACCGATAGCCAA+GGG | 0.608973 | 3.1:+75739514 | None:intergenic |
| AGCAAGTCTTGGCAATGAGG+AGG | 0.615986 | 3.1:-75739394 | MS.gene049358:CDS |
| TCCTACTCAGAAGAACGTAA+AGG | 0.619222 | 3.1:-75739361 | MS.gene049358:CDS |
| TTACTATAGTAGTTGAACAG+TGG | 0.660681 | 3.1:-75739213 | MS.gene049358:intron |
| CCACAAAACCAATTCTGCAG+TGG | 0.661784 | 3.1:-75739307 | MS.gene049358:intron |
| CTGTGGGACCGATAGCCAAG+GGG | 0.713527 | 3.1:+75739515 | None:intergenic |
| TTTGGCGAATGGGATCAGAA+GGG | 0.720121 | 3.1:-75739492 | MS.gene049358:CDS |
| ACAGTGGCCCGAGTTCACAG+GGG | 0.796138 | 3.1:-75739197 | MS.gene049358:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGTTACAACAAATAAAATT+AGG | + | chr3.1:75739457-75739476 | None:intergenic | 15.0% |
| !! | GAATTACTACATAAGTTTAT+AGG | + | chr3.1:75739302-75739321 | None:intergenic | 20.0% |
| ! | ACATATTTGTTTCCTTAACT+AGG | + | chr3.1:75739606-75739625 | None:intergenic | 25.0% |
| ! | AGAATACTGATCATCATAAT+AGG | + | chr3.1:75739759-75739778 | None:intergenic | 25.0% |
| ! | CGAGATTAGATTGATAAATT+AGG | + | chr3.1:75739363-75739382 | None:intergenic | 25.0% |
| ! | TATCTGTATAGCTCAATAAT+GGG | - | chr3.1:75740073-75740092 | MS.gene049358:intron | 25.0% |
| ! | TTATCTGTATAGCTCAATAA+TGG | - | chr3.1:75740072-75740091 | MS.gene049358:intron | 25.0% |
| !! | ATAATTCATGTTTCTGTGTT+AGG | - | chr3.1:75739252-75739271 | MS.gene049358:intron | 25.0% |
| !!! | CATGGATTTTTCGAAAATTA+GGG | - | chr3.1:75739874-75739893 | MS.gene049358:intron | 25.0% |
| AATTCCTCATACTCTATCAT+TGG | - | chr3.1:75740153-75740172 | MS.gene049358:intron | 30.0% | |
| ATACTCTATCATTGGATGAT+AGG | - | chr3.1:75740161-75740180 | MS.gene049358:intron | 30.0% | |
| TTACTATAGTAGTTGAACAG+TGG | - | chr3.1:75740112-75740131 | MS.gene049358:intron | 30.0% | |
| TTGCAGAATAATCACTTCAT+AGG | + | chr3.1:75739423-75739442 | None:intergenic | 30.0% | |
| ! | CCTAATTTTCGAAAAATCCA+TGG | + | chr3.1:75739876-75739895 | None:intergenic | 30.0% |
| !! | CCATGGATTTTTCGAAAATT+AGG | - | chr3.1:75739873-75739892 | MS.gene049358:intron | 30.0% |
| !! | GAAAAATGTTAAGAAGTGTC+CGG | - | chr3.1:75739577-75739596 | MS.gene049358:intron | 30.0% |
| ATCTATACGCTTTCGTTTCT+TGG | - | chr3.1:75739192-75739211 | MS.gene049358:CDS | 35.0% | |
| GAAAAATCCATGGAATAGTC+CGG | + | chr3.1:75739866-75739885 | None:intergenic | 35.0% | |
| TCATCCAATGATAGAGTATG+AGG | + | chr3.1:75740160-75740179 | None:intergenic | 35.0% | |
| !! | TCAATAATGGGTTGTCAACA+TGG | - | chr3.1:75740085-75740104 | MS.gene049358:intron | 35.0% |
| AAACCAGAAGAACCGTTACA+AGG | + | chr3.1:75739530-75739549 | None:intergenic | 40.0% | |
| AAATTCGCCTCTCCTTGTAA+CGG | - | chr3.1:75739515-75739534 | MS.gene049358:CDS | 40.0% | |
| AATGCTTCAAGAGCAAGTCT+TGG | - | chr3.1:75739920-75739939 | MS.gene049358:intron | 40.0% | |
| GAATCTTACCACTGCAGAAT+TGG | + | chr3.1:75740029-75740048 | None:intergenic | 40.0% | |
| TCCTACTCAGAAGAACGTAA+AGG | - | chr3.1:75739964-75739983 | MS.gene049358:intron | 40.0% | |
| TGTTTCCTTAACTAGGCAAC+CGG | + | chr3.1:75739599-75739618 | None:intergenic | 40.0% | |
| ! | AAGACTTGCTCTTGAAGCAT+TGG | + | chr3.1:75739921-75739940 | None:intergenic | 40.0% |
| !! | TGTAACGGTTCTTCTGGTTT+AGG | - | chr3.1:75739530-75739549 | MS.gene049358:intron | 40.0% |
| !!! | GAATTGGTTTTGTGGTAGTG+AGG | + | chr3.1:75740013-75740032 | None:intergenic | 40.0% |
| AAGAGCAAGTCTTGGCAATG+AGG | - | chr3.1:75739928-75739947 | MS.gene049358:intron | 45.0% | |
| AGAAGAACCGTTACAAGGAG+AGG | + | chr3.1:75739525-75739544 | None:intergenic | 45.0% | |
| AGGAATCGAAGCTTGCGATT+CGG | + | chr3.1:75739282-75739301 | None:intergenic | 45.0% | |
| CCACAAAACCAATTCTGCAG+TGG | - | chr3.1:75740018-75740037 | MS.gene049358:intron | 45.0% | |
| GCCTTTACGTTCTTCTGAGT+AGG | + | chr3.1:75739968-75739987 | None:intergenic | 45.0% | |
| TCTCCTTGTAACGGTTCTTC+TGG | - | chr3.1:75739524-75739543 | MS.gene049358:CDS | 45.0% | |
| TTTGGCGAATGGGATCAGAA+GGG | - | chr3.1:75739833-75739852 | MS.gene049358:intron | 45.0% | |
| ! | GAATTTTCCCCTGTGAACTC+GGG | + | chr3.1:75740138-75740157 | None:intergenic | 45.0% |
| ! | GGAATTTTCCCCTGTGAACT+CGG | + | chr3.1:75740139-75740158 | None:intergenic | 45.0% |
| ! | TTTGTGGTAGTGAGGATGCT+GGG | + | chr3.1:75740005-75740024 | None:intergenic | 45.0% |
| !! | TTTTGTGGTAGTGAGGATGC+TGG | + | chr3.1:75740006-75740025 | None:intergenic | 45.0% |
| !!! | CCACTGCAGAATTGGTTTTG+TGG | + | chr3.1:75740021-75740040 | None:intergenic | 45.0% |
| AGCAAGTCTTGGCAATGAGG+AGG | - | chr3.1:75739931-75739950 | MS.gene049358:intron | 50.0% | |
| AGTGTCCGGTTGCCTAGTTA+AGG | - | chr3.1:75739591-75739610 | MS.gene049358:intron | 50.0% | |
| CAATGCAGAAAAACGCCCCT+TGG | - | chr3.1:75739795-75739814 | MS.gene049358:intron | 50.0% | |
| CCATGGAATAGTCCGGCAAT+GGG | + | chr3.1:75739859-75739878 | None:intergenic | 50.0% | |
| GAAAAACGCCCCTTGGCTAT+CGG | - | chr3.1:75739802-75739821 | MS.gene049358:intron | 50.0% | |
| GATAAGAGTGAGCGAGAACG+AGG | + | chr3.1:75739175-75739194 | None:intergenic | 50.0% | |
| GATCCCATTCGCCAAACTGT+GGG | + | chr3.1:75739829-75739848 | None:intergenic | 50.0% | |
| GTTTGGCGAATGGGATCAGA+AGG | - | chr3.1:75739832-75739851 | MS.gene049358:intron | 50.0% | |
| TCCATGGAATAGTCCGGCAA+TGG | + | chr3.1:75739860-75739879 | None:intergenic | 50.0% | |
| TGATCCCATTCGCCAAACTG+TGG | + | chr3.1:75739830-75739849 | None:intergenic | 50.0% | |
| TGGCAATGAGGAGGAGTTTG+CGG | - | chr3.1:75739940-75739959 | MS.gene049358:intron | 50.0% | |
| ! | TTGTGGTAGTGAGGATGCTG+GGG | + | chr3.1:75740004-75740023 | None:intergenic | 50.0% |
| AACAGTGGCCCGAGTTCACA+GGG | - | chr3.1:75740127-75740146 | MS.gene049358:intron | 55.0% | |
| AACTGTGGGACCGATAGCCA+AGG | + | chr3.1:75739815-75739834 | None:intergenic | 55.0% | |
| ACTGTGGGACCGATAGCCAA+GGG | + | chr3.1:75739814-75739833 | None:intergenic | 55.0% | |
| ATGCTGGGGTTCGCTGTGAT+CGG | + | chr3.1:75739990-75740009 | None:intergenic | 55.0% | |
| CCCATTGCCGGACTATTCCA+TGG | - | chr3.1:75739856-75739875 | MS.gene049358:intron | 55.0% | |
| GGTCCCACAGTTTGGCGAAT+GGG | - | chr3.1:75739823-75739842 | MS.gene049358:intron | 55.0% | |
| TGGCTATCGGTCCCACAGTT+TGG | - | chr3.1:75739815-75739834 | MS.gene049358:intron | 55.0% | |
| ACAGTGGCCCGAGTTCACAG+GGG | - | chr3.1:75740128-75740147 | MS.gene049358:intron | 60.0% | |
| CGGTCCCACAGTTTGGCGAA+TGG | - | chr3.1:75739822-75739841 | MS.gene049358:intron | 60.0% | |
| CTGTGGGACCGATAGCCAAG+GGG | + | chr3.1:75739813-75739832 | None:intergenic | 60.0% | |
| GAACAGTGGCCCGAGTTCAC+AGG | - | chr3.1:75740126-75740145 | MS.gene049358:intron | 60.0% | |
| GGATCAGAAGGGCCCATTGC+CGG | - | chr3.1:75739844-75739863 | MS.gene049358:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 75739146 | 75740201 | 75739146 | ID=MS.gene049358 |
| chr3.1 | mRNA | 75739146 | 75740201 | 75739146 | ID=MS.gene049358.t1;Parent=MS.gene049358 |
| chr3.1 | exon | 75740184 | 75740201 | 75740184 | ID=MS.gene049358.t1.exon1;Parent=MS.gene049358.t1 |
| chr3.1 | CDS | 75740184 | 75740201 | 75740184 | ID=cds.MS.gene049358.t1;Parent=MS.gene049358.t1 |
| chr3.1 | exon | 75739308 | 75739544 | 75739308 | ID=MS.gene049358.t1.exon2;Parent=MS.gene049358.t1 |
| chr3.1 | CDS | 75739308 | 75739544 | 75739308 | ID=cds.MS.gene049358.t1;Parent=MS.gene049358.t1 |
| chr3.1 | exon | 75739146 | 75739223 | 75739146 | ID=MS.gene049358.t1.exon3;Parent=MS.gene049358.t1 |
| chr3.1 | CDS | 75739146 | 75739223 | 75739146 | ID=cds.MS.gene049358.t1;Parent=MS.gene049358.t1 |
| Gene Sequence |
| Protein sequence |
