AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31076

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31076.t1 MTR_8g032820 51.786 224 103 3 296 517 95 315 2.88e-72 235
MS.gene31076.t1 MTR_2g049710 41.438 292 115 3 192 483 1 236 3.48e-70 228
MS.gene31076.t1 MTR_1g054530 87.705 122 15 0 271 392 131 252 2.70e-66 216
MS.gene31076.t1 MTR_4g109010 42.437 238 127 4 269 503 920 1150 2.87e-48 181
MS.gene31076.t1 MTR_7g025210 38.655 238 138 4 265 499 67 299 1.93e-47 170
MS.gene31076.t1 MTR_8g009340 36.813 182 103 2 302 483 3 172 1.10e-27 112
MS.gene31076.t1 MTR_0010s0390 85.714 56 8 0 338 393 57 112 2.46e-25 100
MS.gene31076.t1 MTR_1g078060 37.008 127 79 1 389 514 1 127 5.32e-24 98.6
MS.gene31076.t1 MTR_8g467910 34.646 127 82 1 389 514 1 127 1.28e-22 94.4
MS.gene31076.t1 MTR_3g080620 32.308 130 74 1 389 518 1 116 2.05e-15 74.7
MS.gene31076.t1 MTR_1g029390 87.500 40 5 0 354 393 48 87 5.61e-15 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31076.t1 AT1G19260 48.356 517 265 1 1 517 149 663 2.20e-176 517
MS.gene31076.t1 AT3G29763 49.061 479 242 1 10 488 26 502 2.69e-168 487
MS.gene31076.t1 AT4G09660 46.266 482 237 2 36 517 148 607 1.46e-150 447
MS.gene31076.t1 AT3G29765 47.685 432 224 1 86 517 1 430 7.38e-144 426
MS.gene31076.t1 AT1G41920 43.673 490 266 3 33 517 4 488 5.17e-142 419
MS.gene31076.t1 AT3G29450 45.538 437 210 3 31 467 85 493 6.64e-126 379
MS.gene31076.t1 AT2G06541 38.298 517 208 4 1 517 82 487 5.87e-117 358
MS.gene31076.t1 AT2G06541 49.451 273 134 1 1 273 82 350 4.14e-91 290
MS.gene31076.t1 AT3G31402 43.137 153 57 1 36 188 49 171 4.19e-31 125
MS.gene31076.t1 AT3G29794 23.232 396 256 8 88 461 27 396 2.19e-21 97.1
MS.gene31076.t1 AT1G42710 58.462 65 27 0 129 193 119 183 8.85e-19 85.5
MS.gene31076.t1 AT1G42710 32.743 113 74 1 1 111 48 160 1.53e-11 64.3
MS.gene31076.t1 AT4G10200 28.507 221 133 5 7 223 194 393 3.48e-13 72.8
MS.gene31076.t1 AT4G10200 28.507 221 133 5 7 223 194 393 3.48e-13 72.8

Find 79 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCAAAACTTTGATCTTAA+AGG 0.152632 2.4:+5663905 MS.gene31076:CDS
TGATGGCTGGGAACCTTTAT+TGG 0.198760 2.4:+5664484 MS.gene31076:CDS
TTTGCAAAATAGTTCATCTA+TGG 0.319284 2.4:-5663808 None:intergenic
GGAGAAGTTGTTAGTGGAAA+AGG 0.355111 2.4:+5664128 MS.gene31076:CDS
GGTACTGGTGAATGGGAAAT+TGG 0.357784 2.4:+5665487 MS.gene31076:CDS
ACTTCCTTGAAATGCTTAGA+TGG 0.366650 2.4:+5663481 MS.gene31076:CDS
GAAAGTGAAGCGAGTTGTAT+TGG 0.379715 2.4:+5663521 MS.gene31076:CDS
AATTGTTAATGCTTCCTCTC+TGG 0.413755 2.4:+5663593 MS.gene31076:CDS
AAAGTTACCTCCTCCAATAA+AGG 0.415432 2.4:-5664497 None:intergenic
TCGAACAACATTTGTTAAAG+TGG 0.427915 2.4:-5664036 None:intergenic
GAAGATGGAGAAGTTGTTAG+TGG 0.432403 2.4:+5664122 MS.gene31076:CDS
AAGGTCATCAATAATAGCTT+TGG 0.435627 2.4:-5663622 None:intergenic
AAAATAGAATCTTCTAATCA+AGG 0.435739 2.4:+5663456 MS.gene31076:CDS
TCCAAAGTGGGAACTCCAAC+GGG 0.438450 2.4:-5664189 None:intergenic
GTCATCAATAATAGCTTTGG+TGG 0.439699 2.4:-5663619 None:intergenic
AACTAGAGAACAAGTTGGTT+GGG 0.444495 2.4:-5664733 None:intergenic
CATACCATCTAAGCATTTCA+AGG 0.444651 2.4:-5663485 None:intergenic
CATATCTGATAGTGAACAAA+TGG 0.446417 2.4:+5663686 MS.gene31076:CDS
GTTACTGGTATGATTGGTAC+TGG 0.456995 2.4:+5665472 MS.gene31076:CDS
CATCGTCAATGCTATGAACT+TGG 0.459644 2.4:+5664424 MS.gene31076:CDS
GTGAATGGGAAATTGGTGAT+TGG 0.461176 2.4:+5665494 MS.gene31076:CDS
ATTAACTAGAAGGTGATCTC+TGG 0.470064 2.4:-5663994 None:intergenic
ACAGCTTCAAACCTCACAAC+AGG 0.472600 2.4:-5665520 None:intergenic
TTAGAAAGTGAAGGCAATCA+AGG 0.473059 2.4:-5663398 None:intergenic
AAGTATATATCATCCATGTT+TGG 0.473491 2.4:-5664548 None:intergenic
ACTTTAACAAATGTTGTTCG+AGG 0.473718 2.4:+5664038 MS.gene31076:CDS
TTTGTATCGAAGGAGGTTAA+CGG 0.475418 2.4:+5663371 MS.gene31076:CDS
TAGTCGATTGCTTATTTGCA+TGG 0.478360 2.4:+5664700 MS.gene31076:CDS
GAGTTCCCACTTTGGAACTT+TGG 0.478471 2.4:+5664196 MS.gene31076:CDS
GTTAAGAATGCATTGGAAGA+TGG 0.487168 2.4:+5664107 MS.gene31076:CDS
TCATCAATTACAACTTGCAT+TGG 0.489307 2.4:+5663962 MS.gene31076:CDS
AAATTCACTTGGATAAAACT+TGG 0.497849 2.4:-5664783 None:intergenic
AAACACTAAGAGATGATGGC+TGG 0.499431 2.4:+5664471 MS.gene31076:CDS
GATGATGTTTCAGTCAAAGA+TGG 0.501112 2.4:+5664272 MS.gene31076:CDS
AAAGCTATTATTGATGACCT+TGG 0.505716 2.4:+5663624 MS.gene31076:CDS
GAGTTCACCATCCATTCAAA+AGG 0.510239 2.4:+5663569 MS.gene31076:CDS
ACCCGTTGGAGTTCCCACTT+TGG 0.510382 2.4:+5664188 MS.gene31076:CDS
GTATGATTGGTACTGGTGAA+TGG 0.514539 2.4:+5665479 MS.gene31076:CDS
ATGGATGATATATACTTTCG+TGG 0.516377 2.4:+5664554 MS.gene31076:CDS
AAACTAGAGAACAAGTTGGT+TGG 0.518596 2.4:-5664734 None:intergenic
CTGGGAACCTTTATTGGAGG+AGG 0.521262 2.4:+5664490 MS.gene31076:CDS
TTGCGTTATGTTAACAAGCA+TGG 0.526915 2.4:+5663717 MS.gene31076:CDS
CTTCACTTTCTAATGAAGCA+AGG 0.531111 2.4:+5663408 MS.gene31076:CDS
AAGTCTCAAGTTGACTATCA+AGG 0.533316 2.4:-5664826 None:intergenic
TGGCTGGGAACCTTTATTGG+AGG 0.534648 2.4:+5664487 MS.gene31076:CDS
TGGCATTAGCATTCCAAACA+TGG 0.535358 2.4:+5664535 MS.gene31076:CDS
CTTACAACTTCAAGAATTCG+TGG 0.535762 2.4:+5663837 MS.gene31076:CDS
TTACAAACACTAAGAGATGA+TGG 0.536396 2.4:+5664467 MS.gene31076:CDS
AAATAGAATCTTCTAATCAA+GGG 0.543399 2.4:+5663457 MS.gene31076:CDS
AATCAATGTTAAGAATGCAT+TGG 0.546849 2.4:+5664100 MS.gene31076:CDS
TATGATTGGTACTGGTGAAT+GGG 0.547624 2.4:+5665480 MS.gene31076:CDS
TCAAGGAATACCAATGTGGT+TGG 0.552897 2.4:-5664809 None:intergenic
GATGGAAGAGGGGAAGCAAA+TGG 0.553452 2.4:+5664290 MS.gene31076:CDS
TTCCAAAGTGGGAACTCCAA+CGG 0.555554 2.4:-5664190 None:intergenic
GTGGTTGGAGAAAATTCACT+TGG 0.557462 2.4:-5664794 None:intergenic
CTTGCTTCATTAGAAAGTGA+AGG 0.565801 2.4:-5663407 None:intergenic
GTTTCAGTCAAAGATGGAAG+AGG 0.572234 2.4:+5664278 MS.gene31076:CDS
AACACTAAGAGATGATGGCT+GGG 0.577503 2.4:+5664472 MS.gene31076:CDS
GTGATTGGATTCCTGTTGTG+AGG 0.582503 2.4:+5665509 MS.gene31076:CDS
TTTCAGTCAAAGATGGAAGA+GGG 0.583308 2.4:+5664279 MS.gene31076:CDS
CAGTGTACATAAAATGCACA+TGG 0.585780 2.4:-5663933 None:intergenic
CAACTCGTGATTTGTATCGA+AGG 0.588698 2.4:+5663361 MS.gene31076:CDS
TAGCAAACAATTCATCTCCA+AGG 0.589857 2.4:-5663641 None:intergenic
AATTCACCAAAGTTCCAAAG+TGG 0.597424 2.4:-5664202 None:intergenic
TAGAAAGTGAAGGCAATCAA+GGG 0.597719 2.4:-5663397 None:intergenic
GTCAAAACTAGAGAACAAGT+TGG 0.605168 2.4:-5664738 None:intergenic
ACTTCAAGAATTCGTGGACA+AGG 0.619139 2.4:+5663843 MS.gene31076:CDS
AGCTTTGGTGGTTTCCAGAG+AGG 0.623757 2.4:-5663607 None:intergenic
ATTCACCAAAGTTCCAAAGT+GGG 0.625744 2.4:-5664201 None:intergenic
GATGGTGCTAGCAACATGCA+AGG 0.632338 2.4:+5663870 MS.gene31076:CDS
CTCGTGATTTGTATCGAAGG+AGG 0.633610 2.4:+5663364 MS.gene31076:CDS
ATGGTGCTAGCAACATGCAA+GGG 0.649035 2.4:+5663871 MS.gene31076:CDS
AACGTGCTGCTGATACCCGT+TGG 0.662965 2.4:+5664174 MS.gene31076:CDS
TTCAGTCAAAGATGGAAGAG+GGG 0.669637 2.4:+5664280 MS.gene31076:CDS
ATTCGTGGACAAGGTTACGA+TGG 0.699775 2.4:+5663852 MS.gene31076:CDS
CGTGGACAATCAAAACGTGG+AGG 0.713882 2.4:+5664572 MS.gene31076:CDS
CAGTATAAAATAATTCAATG+CGG 0.732730 2.4:-5664628 None:intergenic
ACTATCAAGGAATACCAATG+TGG 0.737295 2.4:-5664813 None:intergenic
TTTCGTGGACAATCAAAACG+TGG 0.753967 2.4:+5664569 MS.gene31076:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAGAATCTTCTAATCA+AGG + chr2.4:5663456-5663475 MS.gene31076:CDS 20.0%
!! AAATAGAATCTTCTAATCAA+GGG + chr2.4:5663457-5663476 MS.gene31076:CDS 20.0%
!! CAGTATAAAATAATTCAATG+CGG - chr2.4:5664631-5664650 None:intergenic 20.0%
!! TAAAAAGAGAATTAACTAGA+AGG - chr2.4:5664007-5664026 None:intergenic 20.0%
!!! ATTAACAATTTCCTTTTGAA+TGG - chr2.4:5663583-5663602 None:intergenic 20.0%
!!! TGAATTATTTTATACTGTTG+TGG + chr2.4:5664634-5664653 MS.gene31076:CDS 20.0%
! AAATTCACTTGGATAAAACT+TGG - chr2.4:5664786-5664805 None:intergenic 25.0%
! AACAGCCATATCATTAAAAA+TGG + chr2.4:5663785-5663804 MS.gene31076:CDS 25.0%
! AAGTATATATCATCCATGTT+TGG - chr2.4:5664551-5664570 None:intergenic 25.0%
! AAGTGATCTATCAAAAAAAC+TGG + chr2.4:5664898-5664917 MS.gene31076:intron 25.0%
! AATCAATGTTAAGAATGCAT+TGG + chr2.4:5664100-5664119 MS.gene31076:CDS 25.0%
! CATTTGATGAAAAAAGTCTT+AGG + chr2.4:5664356-5664375 MS.gene31076:CDS 25.0%
! TCTCAAAACTTTGATCTTAA+AGG + chr2.4:5663905-5663924 MS.gene31076:CDS 25.0%
! TTTGCAAAATAGTTCATCTA+TGG - chr2.4:5663811-5663830 None:intergenic 25.0%
!! AGTATTGTTGAACGATTTTT+GGG + chr2.4:5663741-5663760 MS.gene31076:CDS 25.0%
!! GAACTCATTTTATCATTTTC+AGG - chr2.4:5663555-5663574 None:intergenic 25.0%
!!! AAGTATTGTTGAACGATTTT+TGG + chr2.4:5663740-5663759 MS.gene31076:CDS 25.0%
!!! TATGAAGTATTTTGACTACA+AGG + chr2.4:5665192-5665211 MS.gene31076:intron 25.0%
!!! TATGGCCATTTTTAATGATA+TGG - chr2.4:5663793-5663812 None:intergenic 25.0%
AAAAACTGGTTGCAACTAAA+AGG + chr2.4:5664912-5664931 MS.gene31076:intron 30.0%
AAGGTCATCAATAATAGCTT+TGG - chr2.4:5663625-5663644 None:intergenic 30.0%
ACTTTAACAAATGTTGTTCG+AGG + chr2.4:5664038-5664057 MS.gene31076:CDS 30.0%
ATGGATGATATATACTTTCG+TGG + chr2.4:5664554-5664573 MS.gene31076:CDS 30.0%
GTAGAATTTGCATCTTTGAA+AGG + chr2.4:5664872-5664891 MS.gene31076:intron 30.0%
TATTCAACGATTTCAGAAGA+TGG + chr2.4:5665138-5665157 MS.gene31076:intron 30.0%
TCATCAATTACAACTTGCAT+TGG + chr2.4:5663962-5663981 MS.gene31076:CDS 30.0%
TCATTGCTAACTTCAAAAGT+CGG - chr2.4:5664961-5664980 None:intergenic 30.0%
TTACAAACACTAAGAGATGA+TGG + chr2.4:5664467-5664486 MS.gene31076:CDS 30.0%
! AAAGCTATTATTGATGACCT+TGG + chr2.4:5663624-5663643 MS.gene31076:CDS 30.0%
! ACAATTTCCTTTTGAATGGA+TGG - chr2.4:5663579-5663598 None:intergenic 30.0%
! CATATCTGATAGTGAACAAA+TGG + chr2.4:5663686-5663705 MS.gene31076:CDS 30.0%
! GATGAACTATTTTGCAAACA+TGG + chr2.4:5663813-5663832 MS.gene31076:CDS 30.0%
! TCGAACAACATTTGTTAAAG+TGG - chr2.4:5664039-5664058 None:intergenic 30.0%
!! TGTGTTCAAAAAGGTTGATA+AGG + chr2.4:5665108-5665127 MS.gene31076:intron 30.0%
!!! ACTTTATTTTGCAACAAGCA+TGG + chr2.4:5664515-5664534 MS.gene31076:CDS 30.0%
AAAGTTACCTCCTCCAATAA+AGG - chr2.4:5664500-5664519 None:intergenic 35.0%
AATTCACCAAAGTTCCAAAG+TGG - chr2.4:5664205-5664224 None:intergenic 35.0%
AATTGTTAATGCTTCCTCTC+TGG + chr2.4:5663593-5663612 MS.gene31076:CDS 35.0%
ACTATCAAGGAATACCAATG+TGG - chr2.4:5664816-5664835 None:intergenic 35.0%
ACTTCCTTGAAATGCTTAGA+TGG + chr2.4:5663481-5663500 MS.gene31076:CDS 35.0%
ATTAACTAGAAGGTGATCTC+TGG - chr2.4:5663997-5664016 None:intergenic 35.0%
ATTCACCAAAGTTCCAAAGT+GGG - chr2.4:5664204-5664223 None:intergenic 35.0%
CAGTGTACATAAAATGCACA+TGG - chr2.4:5663936-5663955 None:intergenic 35.0%
CATACCATCTAAGCATTTCA+AGG - chr2.4:5663488-5663507 None:intergenic 35.0%
CTTACAACTTCAAGAATTCG+TGG + chr2.4:5663837-5663856 MS.gene31076:CDS 35.0%
CTTCACTTTCTAATGAAGCA+AGG + chr2.4:5663408-5663427 MS.gene31076:CDS 35.0%
CTTGCTTCATTAGAAAGTGA+AGG - chr2.4:5663410-5663429 None:intergenic 35.0%
GAACAGATTGCGTAATCAAA+TGG + chr2.4:5665039-5665058 MS.gene31076:intron 35.0%
GATGATGTTTCAGTCAAAGA+TGG + chr2.4:5664272-5664291 MS.gene31076:CDS 35.0%
GTCAAAACTAGAGAACAAGT+TGG - chr2.4:5664741-5664760 None:intergenic 35.0%
GTTAAGAATGCATTGGAAGA+TGG + chr2.4:5664107-5664126 MS.gene31076:CDS 35.0%
TAGAAAGTGAAGGCAATCAA+GGG - chr2.4:5663400-5663419 None:intergenic 35.0%
TAGCAAACAATTCATCTCCA+AGG - chr2.4:5663644-5663663 None:intergenic 35.0%
TAGTCGATTGCTTATTTGCA+TGG + chr2.4:5664700-5664719 MS.gene31076:CDS 35.0%
TGAGAGAGATGTGTTCAAAA+AGG + chr2.4:5665099-5665118 MS.gene31076:intron 35.0%
TTAGAAAGTGAAGGCAATCA+AGG - chr2.4:5663401-5663420 None:intergenic 35.0%
TTGCGTTATGTTAACAAGCA+TGG + chr2.4:5663717-5663736 MS.gene31076:CDS 35.0%
TTTCAGTCAAAGATGGAAGA+GGG + chr2.4:5664279-5664298 MS.gene31076:CDS 35.0%
TTTGTATCGAAGGAGGTTAA+CGG + chr2.4:5663371-5663390 MS.gene31076:CDS 35.0%
! ATGTGTGTTGGAAAGAGTTT+TGG + chr2.4:5665450-5665469 MS.gene31076:intron 35.0%
! TGAATTTTCTCCAACCACAT+TGG + chr2.4:5664799-5664818 MS.gene31076:CDS 35.0%
! TTTTCATCATGACCACGAAA+AGG - chr2.4:5663438-5663457 None:intergenic 35.0%
!! AAACTAGAGAACAAGTTGGT+TGG - chr2.4:5664737-5664756 None:intergenic 35.0%
!! AACTAGAGAACAAGTTGGTT+GGG - chr2.4:5664736-5664755 None:intergenic 35.0%
!! AAGTCTCAAGTTGACTATCA+AGG - chr2.4:5664829-5664848 None:intergenic 35.0%
!! GTCATCAATAATAGCTTTGG+TGG - chr2.4:5663622-5663641 None:intergenic 35.0%
!! TATGATTGGTACTGGTGAAT+GGG + chr2.4:5665480-5665499 MS.gene31076:CDS 35.0%
!!! CTTGTTCTCTAGTTTTGACA+AGG + chr2.4:5664742-5664761 MS.gene31076:CDS 35.0%
!!! GTTTTAGGATCGAAGATGTT+GGG + chr2.4:5665348-5665367 MS.gene31076:intron 35.0%
!!! GTTTTGGTTACTGGTATGAT+TGG + chr2.4:5665466-5665485 MS.gene31076:intron 35.0%
!!! TTGGAAAGAGTTTTGGTTAC+TGG + chr2.4:5665457-5665476 MS.gene31076:intron 35.0%
ACTTCAAGAATTCGTGGACA+AGG + chr2.4:5663843-5663862 MS.gene31076:CDS 40.0%
ATCTTCGATCCTAAAACCAG+TGG - chr2.4:5665345-5665364 None:intergenic 40.0%
CACAATCTTCATAGCAGAAG+AGG - chr2.4:5665017-5665036 None:intergenic 40.0%
CAGATTGCGTAATCAAATGG+CGG + chr2.4:5665042-5665061 MS.gene31076:intron 40.0%
CATCGTCAATGCTATGAACT+TGG + chr2.4:5664424-5664443 MS.gene31076:CDS 40.0%
GAAAGTGAAGCGAGTTGTAT+TGG + chr2.4:5663521-5663540 MS.gene31076:CDS 40.0%
GAAGATGGAGAAGTTGTTAG+TGG + chr2.4:5664122-5664141 MS.gene31076:CDS 40.0%
GAGTTCACCATCCATTCAAA+AGG + chr2.4:5663569-5663588 MS.gene31076:CDS 40.0%
GGAGAAGTTGTTAGTGGAAA+AGG + chr2.4:5664128-5664147 MS.gene31076:CDS 40.0%
GTGGTTGGAGAAAATTCACT+TGG - chr2.4:5664797-5664816 None:intergenic 40.0%
GTTACTGGTATGATTGGTAC+TGG + chr2.4:5665472-5665491 MS.gene31076:CDS 40.0%
GTTTCAGTCAAAGATGGAAG+AGG + chr2.4:5664278-5664297 MS.gene31076:CDS 40.0%
TAGAGTGGTAACAGATGTGT+TGG + chr2.4:5665372-5665391 MS.gene31076:intron 40.0%
TCAAGGAATACCAATGTGGT+TGG - chr2.4:5664812-5664831 None:intergenic 40.0%
TGGCATTAGCATTCCAAACA+TGG + chr2.4:5664535-5664554 MS.gene31076:CDS 40.0%
TTCAGTCAAAGATGGAAGAG+GGG + chr2.4:5664280-5664299 MS.gene31076:CDS 40.0%
TTTCGTGGACAATCAAAACG+TGG + chr2.4:5664569-5664588 MS.gene31076:CDS 40.0%
! AAACACTAAGAGATGATGGC+TGG + chr2.4:5664471-5664490 MS.gene31076:CDS 40.0%
! AACACTAAGAGATGATGGCT+GGG + chr2.4:5664472-5664491 MS.gene31076:CDS 40.0%
! AACATGCAAGGGCAATTTTC+TGG + chr2.4:5663882-5663901 MS.gene31076:CDS 40.0%
! CAACTCGTGATTTGTATCGA+AGG + chr2.4:5663361-5663380 MS.gene31076:CDS 40.0%
! GTGAATGGGAAATTGGTGAT+TGG + chr2.4:5665494-5665513 MS.gene31076:CDS 40.0%
!! GTATGATTGGTACTGGTGAA+TGG + chr2.4:5665479-5665498 MS.gene31076:CDS 40.0%
!!! GGTTTTAGGATCGAAGATGT+TGG + chr2.4:5665347-5665366 MS.gene31076:intron 40.0%
ACAGCTTCAAACCTCACAAC+AGG - chr2.4:5665523-5665542 None:intergenic 45.0%
ATTCGTGGACAAGGTTACGA+TGG + chr2.4:5663852-5663871 MS.gene31076:CDS 45.0%
GTGATTGGATTCCTGTTGTG+AGG + chr2.4:5665509-5665528 MS.gene31076:CDS 45.0%
GTTGTGATTGCTTTGTGCGA+TGG + chr2.4:5665414-5665433 MS.gene31076:intron 45.0%
TGATGGCTGGGAACCTTTAT+TGG + chr2.4:5664484-5664503 MS.gene31076:CDS 45.0%
TTCCAAAGTGGGAACTCCAA+CGG - chr2.4:5664193-5664212 None:intergenic 45.0%
! AAGCAAGGATTGCCTTTTCG+TGG + chr2.4:5663423-5663442 MS.gene31076:CDS 45.0%
! ATGGTGCTAGCAACATGCAA+GGG + chr2.4:5663871-5663890 MS.gene31076:CDS 45.0%
! CTCGTGATTTGTATCGAAGG+AGG + chr2.4:5663364-5663383 MS.gene31076:CDS 45.0%
! TCGAAGATGTTGGGTTAGAG+TGG + chr2.4:5665357-5665376 MS.gene31076:intron 45.0%
!! GAGTTCCCACTTTGGAACTT+TGG + chr2.4:5664196-5664215 MS.gene31076:CDS 45.0%
!! GGTACTGGTGAATGGGAAAT+TGG + chr2.4:5665487-5665506 MS.gene31076:CDS 45.0%
AGCAGAAGAGGCTCGTTCAA+CGG - chr2.4:5665005-5665024 None:intergenic 50.0%
CAACACACATAACCACAGCC+AGG - chr2.4:5665440-5665459 None:intergenic 50.0%
CGTGGACAATCAAAACGTGG+AGG + chr2.4:5664572-5664591 MS.gene31076:CDS 50.0%
CTCTACAATTGCTCCCTACG+AGG - chr2.4:5665166-5665185 None:intergenic 50.0%
CTGGCTGTGGTTATGTGTGT+TGG + chr2.4:5665438-5665457 MS.gene31076:intron 50.0%
CTGGGAACCTTTATTGGAGG+AGG + chr2.4:5664490-5664509 MS.gene31076:CDS 50.0%
GATGGAAGAGGGGAAGCAAA+TGG + chr2.4:5664290-5664309 MS.gene31076:CDS 50.0%
TCAGAAGATGGCACCTCGTA+GGG + chr2.4:5665150-5665169 MS.gene31076:intron 50.0%
TCCAAAGTGGGAACTCCAAC+GGG - chr2.4:5664192-5664211 None:intergenic 50.0%
TGGCTGGGAACCTTTATTGG+AGG + chr2.4:5664487-5664506 MS.gene31076:CDS 50.0%
TTCAGAAGATGGCACCTCGT+AGG + chr2.4:5665149-5665168 MS.gene31076:intron 50.0%
! AGCTTTGGTGGTTTCCAGAG+AGG - chr2.4:5663610-5663629 None:intergenic 50.0%
! GATGGTGCTAGCAACATGCA+AGG + chr2.4:5663870-5663889 MS.gene31076:CDS 50.0%
! TCAACGGTTGCTGTTGCAAC+AGG - chr2.4:5664989-5665008 None:intergenic 50.0%
AAGTATGAACCCCCTGGTCC+AGG + chr2.4:5665307-5665326 MS.gene31076:intron 55.0%
ACCCGTTGGAGTTCCCACTT+TGG + chr2.4:5664188-5664207 MS.gene31076:CDS 55.0%
CTAAAACCAGTGGCGTTGCC+AGG - chr2.4:5665335-5665354 None:intergenic 55.0%
GATTGCTTTGTGCGATGGCC+TGG + chr2.4:5665419-5665438 MS.gene31076:intron 55.0%
GTTCGCAAGTATGAACCCCC+TGG + chr2.4:5665301-5665320 MS.gene31076:intron 55.0%
! AACGTGCTGCTGATACCCGT+TGG + chr2.4:5664174-5664193 MS.gene31076:CDS 55.0%
!!! CTGGCAACGCCACTGGTTTT+AGG + chr2.4:5665333-5665352 MS.gene31076:intron 55.0%
GTTGCCAGGAACCTGGACCA+GGG - chr2.4:5665321-5665340 None:intergenic 60.0%
TTGCCAGGAACCTGGACCAG+GGG - chr2.4:5665320-5665339 None:intergenic 60.0%
TTTGTGCGATGGCCTGGCTG+TGG + chr2.4:5665425-5665444 MS.gene31076:intron 60.0%
AACCCCCTGGTCCAGGTTCC+TGG + chr2.4:5665314-5665333 MS.gene31076:intron 65.0%
CAGGTTCCTGGCAACGCCAC+TGG + chr2.4:5665326-5665345 MS.gene31076:intron 65.0%
CAGTGGCGTTGCCAGGAACC+TGG - chr2.4:5665328-5665347 None:intergenic 65.0%
CGTTGCCAGGAACCTGGACC+AGG - chr2.4:5665322-5665341 None:intergenic 65.0%
TGCCAGGAACCTGGACCAGG+GGG - chr2.4:5665319-5665338 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 5663312 5665564 5663312 ID=MS.gene31076
chr2.4 mRNA 5663312 5665564 5663312 ID=MS.gene31076.t1;Parent=MS.gene31076
chr2.4 exon 5663312 5664863 5663312 ID=MS.gene31076.t1.exon1;Parent=MS.gene31076.t1
chr2.4 CDS 5663312 5664863 5663312 ID=cds.MS.gene31076.t1;Parent=MS.gene31076.t1
chr2.4 exon 5665467 5665564 5665467 ID=MS.gene31076.t1.exon2;Parent=MS.gene31076.t1
chr2.4 CDS 5665467 5665564 5665467 ID=cds.MS.gene31076.t1;Parent=MS.gene31076.t1
Gene Sequence

>MS.gene31076

ATGAAACAAGATCAACATATTGAAGTTGTGTTGTCTAAGCAGTCTGAAACAACTCGTGATTTGTATCGAAGGAGGTTAACGGCTTCCCTTGATTGCCTTCACTTTCTAATGAAGCAAGGATTGCCTTTTCGTGGTCATGATGAAAAAATAGAATCTTCTAATCAAGGGAACTTCCTTGAAATGCTTAGATGGTATGCTGACAAAAAAAAGAAAGTGAAGCGAGTTGTATTGGAAAATGCTCCTGAAAATGATAAAATGAGTTCACCATCCATTCAAAAGGAAATTGTTAATGCTTCCTCTCTGGAAACCACCAAAGCTATTATTGATGACCTTGGAGATGAATTGTTTGCTATTCTTGTTGACGAATCAAGAGACATATCTGATAGTGAACAAATGGCTGTTGTTTTGCGTTATGTTAACAAGCATGGAAGTATTGTTGAACGATTTTTGGGTATTGTTCATGTTAAACTTACAACAGCCATATCATTAAAAATGGCCATAGATGAACTATTTTGCAAACATGGACTTACAACTTCAAGAATTCGTGGACAAGGTTACGATGGTGCTAGCAACATGCAAGGGCAATTTTCTGGTCTCAAAACTTTGATCTTAAAGGAAAGTCCATGTGCATTTTATGTACACTGTTTTGCTCATCAATTACAACTTGCATTGGTTGCTATTTCCAGAGATCACCTTCTAGTTAATTCTCTTTTTAGCACAATATCCACTTTAACAAATGTTGTTCGAGGATCTTGCAAACGTACTGACATGCTTCACGAACAACAAAGAATCAATGTTAAGAATGCATTGGAAGATGGAGAAGTTGTTAGTGGAAAAGGTTTGAATCAAGCAAGAAATATGAAACGTGCTGCTGATACCCGTTGGAGTTCCCACTTTGGAACTTTGGTGAATTTGATATTGATGTATAGTTCTGTAATTGATGTTCTTAAAATGTTGAAAGATGATGTTTCAGTCAAAGATGGAAGAGGGGAAGCAAATGGTCTTTTACTTTTAATGAATAATTTTGATTTTGCTATCACATTGCATTTGATGAAAAAAGTCTTAGGTATTTCAAATGATTTGTCACAAGCATTGCAGAGAAAAGATCAAGACATCGTCAATGCTATGAACTTGGTTAGCATTACAAAAGAGTTGTTACAAACACTAAGAGATGATGGCTGGGAACCTTTATTGGAGGAGGTAACTTTATTTTGCAACAAGCATGGCATTAGCATTCCAAACATGGATGATATATACTTTCGTGGACAATCAAAACGTGGAGGTAATGTTGATTCTATCACAATAGAGAGTCATTACCGCATTGAATTATTTTATACTGTTGTGGATATGCAGCTTCAAGAACTTAATAATCGTTTTAATGAGACAAATAGTCGATTGCTTATTTGCATGGAGTGTTTGAGCCCAACCAACTTGTTCTCTAGTTTTGACAAGGCAAAGTTGATTGAATTTGCCAAGTTTTATCCAAGTGAATTTTCTCCAACCACATTGGTATTCCTTGATAGTCAACTTGAGACTTACATCTCTGATATGCGTAAGAGTGTAGAATTTGCATCTTTGAAAGGAATAAGTGATCTATCAAAAAAACTGGTTGCAACTAAAAGGCATATTACTTATTTGTTCGTCTACCGACTTTTGAAGTTAGCAATGATTTTACCTGTTGCAACAGCAACCGTTGAACGAGCCTCTTCTGCTATGAAGATTGTGAAGAACAGATTGCGTAATCAAATGGCGGATGATTTTCTAAACGATTGTCTTGTGACTTACATTGAGAGAGATGTGTTCAAAAAGGTTGATAAGGAGTTGATTATTCAACGATTTCAGAAGATGGCACCTCGTAGGGAGCAATTGTAGAGTATGCATATGAAGTATTTTGACTACAAGGTACACTGAATGCACTGTAATTTTTTGAATATATATTATTATAATTATTAGTAATTAAATATATTATTATAATTATTAGTAATTAAAGTTCGCAAGTATGAACCCCCTGGTCCAGGTTCCTGGCAACGCCACTGGTTTTAGGATCGAAGATGTTGGGTTAGAGTGGTAACAGATGTGTTGGTTTTGATTTAACTGACTGTGTTGTGATTGCTTTGTGCGATGGCCTGGCTGTGGTTATGTGTGTTGGAAAGAGTTTTGGTTACTGGTATGATTGGTACTGGTGAATGGGAAATTGGTGATTGGATTCCTGTTGTGAGGTTTGAAGCTGTGCTTGTGTGTTACTGTGTGTGA
Protein sequence

>MS.gene31076.t1

MKQDQHIEVVLSKQSETTRDLYRRRLTASLDCLHFLMKQGLPFRGHDEKIESSNQGNFLEMLRWYADKKKKVKRVVLENAPENDKMSSPSIQKEIVNASSLETTKAIIDDLGDELFAILVDESRDISDSEQMAVVLRYVNKHGSIVERFLGIVHVKLTTAISLKMAIDELFCKHGLTTSRIRGQGYDGASNMQGQFSGLKTLILKESPCAFYVHCFAHQLQLALVAISRDHLLVNSLFSTISTLTNVVRGSCKRTDMLHEQQRINVKNALEDGEVVSGKGLNQARNMKRAADTRWSSHFGTLVNLILMYSSVIDVLKMLKDDVSVKDGRGEANGLLLLMNNFDFAITLHLMKKVLGISNDLSQALQRKDQDIVNAMNLVSITKELLQTLRDDGWEPLLEEVTLFCNKHGISIPNMDDIYFRGQSKRGGNVDSITIESHYRIELFYTVVDMQLQELNNRFNETNSRLLICMECLSPTNLFSSFDKAKLIEFAKFYPSEFSPTTLVFLDSQLETYISDMLLVTGMIGTGEWEIGDWIPVVRFEAVLVCYCV