AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052447


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052447.t1 MTR_0961s0010 61.224 196 19 3 1 139 1 196 1.89e-27 100
MS.gene052447.t1 MTR_0961s0010 71.233 146 14 2 22 139 1 146 7.03e-18 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 21 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGCCTTCGAAGTTCTTTA+CGG 0.344809 6.1:+75369206 None:intergenic
AAGTTGAAATTCTGGAAATA+AGG 0.355807 6.1:-75369162 MS.gene052447:CDS
TTCAATCACTTCATCATAAT+AGG 0.366272 6.1:+75369447 None:intergenic
GGAAGCTGAAAGGAAGGAAA+AGG 0.444058 6.1:-75369098 MS.gene052447:CDS
AGAGGAGGAAGCTGAAAGGA+AGG 0.454150 6.1:-75369104 MS.gene052447:CDS
TCGTGATTTAAATCAGCATC+AGG 0.464071 6.1:+75369400 None:intergenic
AGAAATCTAAGCCAACTACT+TGG 0.472779 6.1:-75369670 MS.gene052447:CDS
AGGAGGAAGCTGAAATGAAA+AGG 0.473270 6.1:-75369132 MS.gene052447:CDS
GGAAGCTGAAATGAAAAGGA+AGG 0.493440 6.1:-75369128 MS.gene052447:CDS
TCTGGAAATAAGGAAGGAAA+AGG 0.493692 6.1:-75369152 MS.gene052447:CDS
AGCTGAAAGGAAGGAAAAGG+AGG 0.497172 6.1:-75369095 MS.gene052447:CDS
AGGAAGAGGAGGAAGCTGAA+AGG 0.516462 6.1:-75369108 MS.gene052447:CDS
GAAATCTAAGCCAACTACTT+GGG 0.528517 6.1:-75369669 MS.gene052447:intron
TGAAATTCTGGAAATAAGGA+AGG 0.531101 6.1:-75369158 MS.gene052447:CDS
TGAAATGAAAAGGAAGGAAG+AGG 0.532757 6.1:-75369122 MS.gene052447:CDS
CCTGAGATGACCCAAGTAGT+TGG 0.545425 6.1:+75369659 None:intergenic
GGAAATAAGGAAGGAAAAGG+AGG 0.550433 6.1:-75369149 MS.gene052447:CDS
AATGAAAAGGAAGGAAGAGG+AGG 0.596938 6.1:-75369119 MS.gene052447:CDS
AAAACCGTAAAGAACTTCGA+AGG 0.658384 6.1:-75369210 MS.gene052447:CDS
TGATGCTGATTTAAATCACG+AGG 0.690474 6.1:-75369398 MS.gene052447:CDS
TCTTCCACAAATGACTGACG+AGG 0.715715 6.1:-75369693 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTATAGAAAATAATATTAAA+GGG + chr6.1:75369158-75369177 None:intergenic 10.0%
!! ATAATATTAAAGGGATAAAA+CGG + chr6.1:75369149-75369168 None:intergenic 15.0%
!! GAAAAAGCAAATTAAAAAAA+AGG - chr6.1:75369459-75369478 MS.gene052447:CDS 15.0%
!! GGTATAGAAAATAATATTAA+AGG + chr6.1:75369159-75369178 None:intergenic 15.0%
!!! ATTTTTTAATATAAGTTTGC+CGG + chr6.1:75369052-75369071 None:intergenic 15.0%
!! GAAAAAGAAAATGAAAAAGA+AGG - chr6.1:75369621-75369640 MS.gene052447:intron 20.0%
! AAAAAAGGAAGTTGAAATTC+TGG - chr6.1:75369474-75369493 MS.gene052447:intron 25.0%
! TTCAATCACTTCATCATAAT+AGG + chr6.1:75369200-75369219 None:intergenic 25.0%
!! AAGTTGAAATTCTGGAAATA+AGG - chr6.1:75369482-75369501 MS.gene052447:intron 25.0%
!! CTTTTGCCATAATTATATGT+GGG + chr6.1:75369012-75369031 None:intergenic 25.0%
!! TGAAATTCTGAAAAAAAGCA+AGG - chr6.1:75369576-75369595 MS.gene052447:intron 25.0%
AAAAAATGATACAGATCACC+AGG - chr6.1:75369065-75369084 MS.gene052447:CDS 30.0%
CCACATATAATTATGGCAAA+AGG - chr6.1:75369010-75369029 MS.gene052447:CDS 30.0%
CTAAAAAAGTTTGTGAGGAA+GGG - chr6.1:75369668-75369687 MS.gene052447:intron 30.0%
GAAAAAAAGCAAGGAAAAGA+AGG - chr6.1:75369585-75369604 MS.gene052447:intron 30.0%
TAAAAAAGTTTGTGAGGAAG+GGG - chr6.1:75369669-75369688 MS.gene052447:intron 30.0%
! CCTTTTGCCATAATTATATG+TGG + chr6.1:75369013-75369032 None:intergenic 30.0%
! TGAAATTCTGGAAATAAGGA+AGG - chr6.1:75369486-75369505 MS.gene052447:intron 30.0%
!!! AATATAAGTTTGCCGGTTTT+TGG + chr6.1:75369045-75369064 None:intergenic 30.0%
AAAACCGTAAAGAACTTCGA+AGG - chr6.1:75369434-75369453 MS.gene052447:CDS 35.0%
AATTATGGCAAAAGGAGATC+TGG - chr6.1:75369018-75369037 MS.gene052447:CDS 35.0%
ACGATCAAAAAAATCATGCC+TGG + chr6.1:75369086-75369105 None:intergenic 35.0%
AGAAATCTAAGCCAACTACT+TGG - chr6.1:75368974-75368993 MS.gene052447:CDS 35.0%
AGCAGCTAAAAAAGTTTGTG+AGG - chr6.1:75369663-75369682 MS.gene052447:intron 35.0%
AGGAATCAAAAAACTTCTGC+AGG + chr6.1:75369180-75369199 None:intergenic 35.0%
ATTATGGCAAAAGGAGATCT+GGG - chr6.1:75369019-75369038 MS.gene052447:CDS 35.0%
GAAATCTAAGCCAACTACTT+GGG - chr6.1:75368975-75368994 MS.gene052447:CDS 35.0%
GCTAAAAAAGTTTGTGAGGA+AGG - chr6.1:75369667-75369686 MS.gene052447:intron 35.0%
TCAGGACCCACATATAATTA+TGG - chr6.1:75369003-75369022 MS.gene052447:CDS 35.0%
TCGTGATTTAAATCAGCATC+AGG + chr6.1:75369247-75369266 None:intergenic 35.0%
TCTGGAAATAAGGAAGGAAA+AGG - chr6.1:75369492-75369511 MS.gene052447:intron 35.0%
TGAAATGAAAAGGAAGGAAG+AGG - chr6.1:75369522-75369541 MS.gene052447:intron 35.0%
! TCGAAGTTCTTTACGGTTTT+CGG + chr6.1:75369434-75369453 None:intergenic 35.0%
!! TGATGCTGATTTAAATCACG+AGG - chr6.1:75369246-75369265 MS.gene052447:intron 35.0%
AATGAAAAGGAAGGAAGAGG+AGG - chr6.1:75369525-75369544 MS.gene052447:intron 40.0%
AGGAGGAAGCTGAAATGAAA+AGG - chr6.1:75369512-75369531 MS.gene052447:intron 40.0%
CTCTAAACCAGCAGTTGTTT+AGG - chr6.1:75369362-75369381 MS.gene052447:intron 40.0%
GGAAATAAGGAAGGAAAAGG+AGG - chr6.1:75369495-75369514 MS.gene052447:intron 40.0%
GGAAGCTGAAATGAAAAGGA+AGG - chr6.1:75369516-75369535 MS.gene052447:intron 40.0%
TCAGCCTTCGAAGTTCTTTA+CGG + chr6.1:75369441-75369460 None:intergenic 40.0%
! TTTTCAGCCTAAACAACTGC+TGG + chr6.1:75369372-75369391 None:intergenic 40.0%
!! CTAAACAACTGCTGGTTTAG+AGG + chr6.1:75369364-75369383 None:intergenic 40.0%
AGCTGAAAGGAAGGAAAAGG+AGG - chr6.1:75369549-75369568 MS.gene052447:intron 45.0%
GGAAGCTGAAAGGAAGGAAA+AGG - chr6.1:75369546-75369565 MS.gene052447:intron 45.0%
TGTTTAGGCTGAAAACTGCG+TGG - chr6.1:75369377-75369396 MS.gene052447:intron 45.0%
! AAAACTGCGTGGTTTGGCAA+AGG - chr6.1:75369388-75369407 MS.gene052447:intron 45.0%
AGAGGAGGAAGCTGAAAGGA+AGG - chr6.1:75369540-75369559 MS.gene052447:intron 50.0%
AGGAAGAGGAGGAAGCTGAA+AGG - chr6.1:75369536-75369555 MS.gene052447:intron 50.0%
AGGAGATCTGGGCCAAAAAC+CGG - chr6.1:75369030-75369049 MS.gene052447:CDS 50.0%
AGGCTGAAAACTGCGTGGTT+TGG - chr6.1:75369382-75369401 MS.gene052447:intron 50.0%
CCAACTACTTGGGTCATCTC+AGG - chr6.1:75368985-75369004 MS.gene052447:CDS 50.0%
CCTGAGATGACCCAAGTAGT+TGG + chr6.1:75368988-75369007 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 75368961 75369705 75368961 ID=MS.gene052447
chr6.1 mRNA 75368961 75369705 75368961 ID=MS.gene052447.t1;Parent=MS.gene052447
chr6.1 exon 75369670 75369705 75369670 ID=MS.gene052447.t1.exon1;Parent=MS.gene052447.t1
chr6.1 CDS 75369670 75369705 75369670 ID=cds.MS.gene052447.t1;Parent=MS.gene052447.t1
chr6.1 exon 75369395 75369482 75369395 ID=MS.gene052447.t1.exon2;Parent=MS.gene052447.t1
chr6.1 CDS 75369395 75369482 75369395 ID=cds.MS.gene052447.t1;Parent=MS.gene052447.t1
chr6.1 exon 75368961 75369256 75368961 ID=MS.gene052447.t1.exon3;Parent=MS.gene052447.t1
chr6.1 CDS 75368961 75369256 75368961 ID=cds.MS.gene052447.t1;Parent=MS.gene052447.t1
Gene Sequence

>MS.gene052447

ATGACTGACGAGGAGAAATCTAAGCCAACTACTTGGGTCATCTCAGGACCCACATATAATTATGGCAAAAGGAGATCTGGGCCAAAAACCGGCAAACTTATATTAAAAAATGATACAGATCACCAGGCATGATTTTTTTGATCGTTAGCTATGCTTTTTATTCAAACAATTTTTAGTTTCTTTGTCCGTTTTATCCCTTTAATATTATTTTCTATACCTGCAGAAGTTTTTTGATTCCTATTATGATGAAGTGATTGAAGACGTTCGTAGTTGCATCAATAAGCCTGATGCTGATTTAAATCACGAGGCAAGTTTTTCTCGAAGAACAATGTTTTTTATTCAAGTTTTACAGTTTTAGTTTCTTTGTTCATATTTGAGTTCTTTAATATTCATTAATTATCCTCTAAACCAGCAGTTGTTTAGGCTGAAAACTGCGTGGTTTGGCAAAGGTCATTATAGAGATTATGTTGCCGAAAACCGTAAAGAACTTCGAAGGCTGAAAAAGCAAATTAAAAAAAAGGAAGTTGAAATTCTGGAAATAAGGAAGGAAAAGGAGGAAGCTGAAATGAAAAGGAAGGAAGAGGAGGAAGCTGAAAGGAAGGAAAAGGAGGAAGCTGAAATTCTGAAAAAAAGCAAGGAAAAGAAGGAAGCTAAAATTCAGAAAAAGAAAATGAAAAAGAAGGAAGCTAAAATTCGAAAGCAAGCAGCTAAAAAAGTTTGTGAGGAAGGGGCTAAAGCATGTTAA

Protein sequence

>MS.gene052447.t1

MTDEEKSKPTTWKFFDSYYDEVIEDVRSCINKPDADLNHEASHYRDYVAENRKELRRLKKQIKKKEVEILEIRKEKEEAEMKRKEEEEAERKEKEEAEILKKSKEKKEAKIQKKKMKKKEAKIRKQAAKKVCEEGAKAC