AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31110.t1 MTR_2g097800 84.308 325 21 6 1 299 1 321 0.0 521
MS.gene31110.t1 MTR_4g064947 38.462 260 127 7 73 299 118 377 4.59e-45 157
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31110.t1 AT5G59050 36.129 310 114 18 25 299 52 312 1.17e-21 93.2
MS.gene31110.t1 AT5G59050 38.000 200 64 13 135 299 17 191 8.06e-11 60.8

Find 69 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTACGCTAACGTGGTTAA+AGG 0.245389 2.4:-6125989 MS.gene31110:CDS
AAATTAAAGCCATTGAATTA+AGG 0.289984 2.4:-6125838 MS.gene31110:CDS
GCACAACTCTTGCTGGTATA+AGG 0.306651 2.4:+6124471 None:intergenic
CCTACGCTAACGTGGTTAAA+GGG 0.390281 2.4:-6125988 MS.gene31110:CDS
AAAGCCTGCACAACTCTTGC+TGG 0.391708 2.4:+6124464 None:intergenic
ACCTGTCCCACTTCTTGAAC+CGG 0.402912 2.4:+6125658 None:intergenic
CTCCGCCAGCTACACCTTGT+TGG 0.404982 2.4:-6126021 MS.gene31110:CDS
GGAGGGACGCCAACAACTTT+GGG 0.411943 2.4:+6124416 None:intergenic
GAGGCAGAATCATATCATTC+TGG 0.418235 2.4:+6122734 None:intergenic
GTCTTCCAACAAGGTGTAGC+TGG 0.435910 2.4:+6126016 None:intergenic
TGGAGGGACGCCAACAACTT+TGG 0.438518 2.4:+6124415 None:intergenic
GAGATTCAGAAGCAAGGTAA+TGG 0.461458 2.4:-6125776 MS.gene31110:CDS
GCAACTACACTTTGACAAAA+CGG 0.470788 2.4:-6124441 MS.gene31110:CDS
CCACTGGTCTGCAATGGAGC+AGG 0.476520 2.4:+6125716 None:intergenic
CCAGTTCGGAATTGTGACTC+TGG 0.478083 2.4:+6126249 None:intergenic
ATATGATTCTGCCTCAAGAA+TGG 0.490516 2.4:-6122726 MS.gene31110:CDS
TATACCAGCAAGAGTTGTGC+AGG 0.500359 2.4:-6124468 MS.gene31110:CDS
GCTCCATTGCAGACCAGTGG+TGG 0.500459 2.4:-6125713 MS.gene31110:CDS
CTGCATCATTCCTCCACCAC+TGG 0.505752 2.4:+6125700 None:intergenic
GTGGGATTTAATTAGTTCAC+CGG 0.508002 2.4:-6126110 MS.gene31110:CDS
TGTAACCAAGGGAGCTCAGA+AGG 0.515050 2.4:-6126061 MS.gene31110:CDS
ATGACCCTTCTGAGCTCCCT+TGG 0.517637 2.4:+6126056 None:intergenic
CCAGAGTCACAATTCCGAAC+TGG 0.519755 2.4:-6126249 MS.gene31110:intron
TCCGGTTCAAGAAGTGGGAC+AGG 0.522917 2.4:-6125659 MS.gene31110:CDS
CCTCCACCACTGGTCTGCAA+TGG 0.524420 2.4:+6125710 None:intergenic
ATCACACAAAGCATTGATTG+AGG 0.524445 2.4:-6125864 MS.gene31110:intron
CCATTGCAGACCAGTGGTGG+AGG 0.531905 2.4:-6125710 MS.gene31110:CDS
AAAACGGCTCCCAAAGTTGT+TGG 0.534159 2.4:-6124425 MS.gene31110:CDS
GGTGGGTCCGGTTCAAGAAG+TGG 0.535246 2.4:-6125665 MS.gene31110:CDS
GTGAAAACATACCTTCCCTA+TGG 0.540528 2.4:+6122794 None:intergenic
ATTCAGAAGCAAGGTAATGG+AGG 0.545729 2.4:-6125773 MS.gene31110:CDS
CACCATGGTTCCAACGGACG+AGG 0.546751 2.4:-6126360 MS.gene31110:CDS
GGAAACATATGGTACCAGTT+CGG 0.547360 2.4:+6126235 None:intergenic
ATCCTCGTCCGTTGGAACCA+TGG 0.552840 2.4:+6126358 None:intergenic
CTCCCTTGGTTACAACTCAC+CGG 0.562393 2.4:+6126070 None:intergenic
CAGTTCGGAATTGTGACTCT+GGG 0.562437 2.4:+6126250 None:intergenic
CAGTGGTGGAGGAATGATGC+AGG 0.565231 2.4:-6125699 MS.gene31110:CDS
GCGACTTCTAAACAGAATCA+TGG 0.566085 2.4:-6125932 MS.gene31110:CDS
GAATGGTACCACGGAGTGGA+GGG 0.567764 2.4:+6124399 None:intergenic
CTTCAATATGTCCATTCTTG+AGG 0.572811 2.4:+6122715 None:intergenic
CAATCACACCATGGTTCCAA+CGG 0.579340 2.4:-6126366 MS.gene31110:CDS
GGATTCAACTCTGTGGTCAC+CGG 0.580045 2.4:-6126089 MS.gene31110:CDS
GTGATGAAATCCTCGTCCGT+TGG 0.582382 2.4:+6126350 None:intergenic
TTACATCAAGCAGCAAAATG+AGG 0.589469 2.4:-6125807 MS.gene31110:CDS
GGTGACCACAGAGTTGAATC+CGG 0.590456 2.4:+6126091 None:intergenic
CTACGCTAACGTGGTTAAAG+GGG 0.595945 2.4:-6125987 MS.gene31110:CDS
GTGGGTCCGGTTCAAGAAGT+GGG 0.596347 2.4:-6125664 MS.gene31110:CDS
AGAATGGTACCACGGAGTGG+AGG 0.600926 2.4:+6124398 None:intergenic
CCCTTTAACCACGTTAGCGT+AGG 0.602141 2.4:+6125988 None:intergenic
AAAGAAGAGATTCAGAAGCA+AGG 0.613101 2.4:-6125782 MS.gene31110:CDS
AGACATTTGATGAGTTAACT+CGG 0.619910 2.4:+6126328 None:intergenic
TTCAGAAGCAAGGTAATGGA+GGG 0.622585 2.4:-6125772 MS.gene31110:CDS
TGATTCTGATGGAGCAACAA+CGG 0.623497 2.4:+6125613 None:intergenic
GGCCTTGTTAGATGAGGACG+AGG 0.624372 2.4:+6125737 None:intergenic
CCTGCTCCATTGCAGACCAG+TGG 0.625104 2.4:-6125716 MS.gene31110:CDS
CACCGGTGAGTTGTAACCAA+GGG 0.632229 2.4:-6126072 MS.gene31110:CDS
GCAACAACGGTTCCAGCACG+AGG 0.632333 2.4:+6125626 None:intergenic
TTCGGAATTGTGACTCTGGG+AGG 0.633765 2.4:+6126253 None:intergenic
TTCCAACAAGGTGTAGCTGG+CGG 0.645177 2.4:+6126019 None:intergenic
GGAGCAGGCCTTGTTAGATG+AGG 0.651849 2.4:+6125731 None:intergenic
GTCCTCGTCCTCATCTAACA+AGG 0.653193 2.4:-6125739 MS.gene31110:CDS
TTAAGAATGGTACCACGGAG+TGG 0.655116 2.4:+6124395 None:intergenic
GTAACCAAGGGAGCTCAGAA+GGG 0.662619 2.4:-6126060 MS.gene31110:CDS
TCTTGCTGGTATAAGGACAG+TGG 0.678497 2.4:+6124478 None:intergenic
GTTGGCGTCCCTCCACTCCG+TGG 0.686225 2.4:-6124407 MS.gene31110:intron
TGCTAACTGAGAGAACATCA+TGG 0.694654 2.4:+6126393 None:intergenic
TCACCGGTGAGTTGTAACCA+AGG 0.715334 2.4:-6126073 MS.gene31110:CDS
GTTCACCGGATTCAACTCTG+TGG 0.734801 2.4:-6126096 MS.gene31110:CDS
TAGCAACAACAATCACACCA+TGG 0.741419 2.4:-6126375 MS.gene31110:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTAATTTAAAT+CGG + chr2.4:6125049-6125068 None:intergenic 0.0%
!! ATATGCATATAAAATTTATT+TGG - chr2.4:6125155-6125174 MS.gene31110:intron 10.0%
!! TAACAATAATAAATTAAGAA+TGG + chr2.4:6124754-6124773 None:intergenic 10.0%
!! TATGCATATAAAATTTATTT+GGG - chr2.4:6125156-6125175 MS.gene31110:intron 10.0%
!!! AATTTTATATGCATATAAAA+TGG + chr2.4:6125151-6125170 None:intergenic 10.0%
!!! ATATTTGATGTTTTTAAAAT+TGG + chr2.4:6124032-6124051 None:intergenic 10.0%
!!! ATTTTATATGCATATAAAAT+GGG + chr2.4:6125169-6125150 None:intergenic 10.0%
!!! CAATTTTAATAGATTTAAAA+TGG - chr2.4:6123973-6123992 MS.gene31110:intron 10.0%
!!! CTTTCTTTTTAAAATTATTT+GGG - chr2.4:6125246-6125265 MS.gene31110:intron 10.0%
!!! TAATTTTAAAGATATTTACA+TGG - chr2.4:6123745-6123764 MS.gene31110:intron 10.0%
!! AAATTAAACAAATGTAGAAA+GGG + chr2.4:6125844-6125863 None:intergenic 15.0%
!! ATTTATAAACAATTTCTACA+TGG - chr2.4:6125397-6125416 MS.gene31110:intron 15.0%
!! CAGTTAAGTATAATTAATTA+AGG + chr2.4:6124548-6124567 None:intergenic 15.0%
!! GGTAAGATTATTTATTTATT+AGG + chr2.4:6124851-6124870 None:intergenic 15.0%
!! TAAATTAAACAAATGTAGAA+AGG + chr2.4:6125845-6125864 None:intergenic 15.0%
!!! AACAATGTTAAATCTTTTTA+AGG - chr2.4:6124953-6124972 MS.gene31110:intron 15.0%
!!! ATACTTTTCTTTAGAAAAAT+TGG - chr2.4:6125979-6125998 MS.gene31110:CDS 15.0%
!!! CCAAATAATTTTAAAAAGAA+AGG + chr2.4:6125248-6125267 None:intergenic 15.0%
!!! CCTTTCTTTTTAAAATTATT+TGG - chr2.4:6125245-6125264 MS.gene31110:intron 15.0%
!!! TTTTATATTATAGAACTAGT+TGG + chr2.4:6126215-6126234 None:intergenic 15.0%
!! AAACTTAGAGTATAAAACTT+AGG + chr2.4:6125277-6125296 None:intergenic 20.0%
!! AAATTAAAGCCATTGAATTA+AGG - chr2.4:6123295-6123314 MS.gene31110:intron 20.0%
!! CAAAGAAGAGTTAATAATAT+AGG + chr2.4:6122977-6122996 None:intergenic 20.0%
!! GATTGATAAGAAAATGAAAT+AGG + chr2.4:6125371-6125390 None:intergenic 20.0%
!! TGATGAAAATCTATCAATAT+TGG + chr2.4:6123253-6123272 None:intergenic 20.0%
!! TTATATATATTGTTAGGGAA+AGG - chr2.4:6124627-6124646 MS.gene31110:intron 20.0%
!!! AAACGAATTTGATATTGTTT+TGG + chr2.4:6124571-6124590 None:intergenic 20.0%
!!! ACTTAAGCGTATTTTATTTT+TGG + chr2.4:6125307-6125326 None:intergenic 20.0%
!!! ATGTTTTTTCCTTAATTCAA+TGG + chr2.4:6123307-6123326 None:intergenic 20.0%
!!! TGAAAGCTTTTTTTTTTTTG+TGG - chr2.4:6124227-6124246 MS.gene31110:intron 20.0%
!!! TGATTTGCAATTCTTAATAA+TGG - chr2.4:6126101-6126120 MS.gene31110:CDS 20.0%
!!! TTTGATGTTTTTAAAATTGG+AGG + chr2.4:6124029-6124048 None:intergenic 20.0%
! AACTAGATTGATGCTATTAA+GGG - chr2.4:6124150-6124169 MS.gene31110:intron 25.0%
! AATTAAGCAGTTAGAAAGTT+TGG - chr2.4:6124485-6124504 MS.gene31110:CDS 25.0%
! ACAGATTCGAATATTAAAAG+TGG + chr2.4:6123648-6123667 None:intergenic 25.0%
! ATAAATTAAGAATGGTACCA+CGG + chr2.4:6124746-6124765 None:intergenic 25.0%
! CAGATTCGAATATTAAAAGT+GGG + chr2.4:6123647-6123666 None:intergenic 25.0%
! GCACGTTATATATATTGTTA+GGG - chr2.4:6124622-6124641 MS.gene31110:intron 25.0%
! TAACTAGATTGATGCTATTA+AGG - chr2.4:6124149-6124168 MS.gene31110:intron 25.0%
! TGAAAATCTATCAATATTGG+AGG + chr2.4:6123250-6123269 None:intergenic 25.0%
! TGCACGTTATATATATTGTT+AGG - chr2.4:6124621-6124640 MS.gene31110:intron 25.0%
! TTTCTACATGGAAAATTCTA+CGG - chr2.4:6125409-6125428 MS.gene31110:intron 25.0%
!! AAACTTAGAGCATTAAACTT+AGG + chr2.4:6125187-6125206 None:intergenic 25.0%
!! TAAGTTTAATGCTCTAAGTT+TGG - chr2.4:6125186-6125205 MS.gene31110:intron 25.0%
!! TACAGGTACTATTTTATTGT+CGG + chr2.4:6125681-6125700 None:intergenic 25.0%
!!! AAGCTTTTTTTTTTTTGTGG+TGG - chr2.4:6124230-6124249 MS.gene31110:intron 25.0%
!!! CTAACTGCTTAATTTTTTGA+TGG + chr2.4:6124479-6124498 None:intergenic 25.0%
!!! TAATTTTTTGATGGCTAGAT+AGG + chr2.4:6124470-6124489 None:intergenic 25.0%
!!! TTTTTTTTTTTTGTGGTGGT+CGG - chr2.4:6124234-6124253 MS.gene31110:intron 25.0%
AAGCTTGTAGAGTTACAAAT+GGG + chr2.4:6124003-6124022 None:intergenic 30.0%
ACTAACATCTCGAATAGATT+AGG + chr2.4:6125001-6125020 None:intergenic 30.0%
ACTAATAAAGAAGACACTTG+AGG + chr2.4:6123840-6123859 None:intergenic 30.0%
AGACATTTGATGAGTTAACT+CGG + chr2.4:6122808-6122827 None:intergenic 30.0%
ATCTTACCCAACAAAATATG+TGG - chr2.4:6124863-6124882 MS.gene31110:intron 30.0%
ATGTTTATGTGACAACTTAG+TGG + chr2.4:6124375-6124394 None:intergenic 30.0%
CAAACAGATGAGAAATTGAT+AGG + chr2.4:6125883-6125902 None:intergenic 30.0%
CCAAGTTAACTAAAAGTCTT+AGG - chr2.4:6124907-6124926 MS.gene31110:intron 30.0%
CGAATAGATTAGGATTACAA+TGG + chr2.4:6124991-6125010 None:intergenic 30.0%
CTTAGAAAGTAGAAAAAGAG+TGG + chr2.4:6123695-6123714 None:intergenic 30.0%
GATGACATGACATATTTAGA+TGG + chr2.4:6124353-6124372 None:intergenic 30.0%
GCAGTCTCGAAAAATAAAAT+TGG + chr2.4:6126141-6126160 None:intergenic 30.0%
GTTTGTGCAATTATTCGTAT+AGG - chr2.4:6125019-6125038 MS.gene31110:intron 30.0%
TAAGCTTGTAGAGTTACAAA+TGG + chr2.4:6124004-6124023 None:intergenic 30.0%
TAGATAGTAAGGGAAACATA+TGG + chr2.4:6122912-6122931 None:intergenic 30.0%
TAGTAATGCTTGCAATATCT+TGG + chr2.4:6125707-6125726 None:intergenic 30.0%
TATTGAATGTCATGTTGCTA+AGG + chr2.4:6125104-6125123 None:intergenic 30.0%
TCAGAATCAACCAAAAAGAA+AGG - chr2.4:6123534-6123553 MS.gene31110:intron 30.0%
TCTTAAGTGTTACACTTTGT+GGG + chr2.4:6125616-6125635 None:intergenic 30.0%
TTAGGAGAGTAGTAACTAAA+CGG + chr2.4:6124833-6124852 None:intergenic 30.0%
! AAATCAAAGTTGTTGCTTGA+TGG + chr2.4:6124783-6124802 None:intergenic 30.0%
! AGTCATGAGAATGACTATTT+TGG - chr2.4:6125435-6125454 MS.gene31110:intron 30.0%
! ATCTCATCCACATATTTTGT+TGG + chr2.4:6124873-6124892 None:intergenic 30.0%
! GATGAATTGATGATACTATG+AGG + chr2.4:6125536-6125555 None:intergenic 30.0%
! TCTCATCCACATATTTTGTT+GGG + chr2.4:6124872-6124891 None:intergenic 30.0%
! TGTTCATGTTTTTCACTAGT+CGG - chr2.4:6123583-6123602 MS.gene31110:intron 30.0%
!! CCTAAGACTTTTAGTTAACT+TGG + chr2.4:6124910-6124929 None:intergenic 30.0%
!!! AGATCTTGTTTTGAACCATA+GGG - chr2.4:6126324-6126343 MS.gene31110:CDS 30.0%
!!! TTCTTTTTGGTTGATTCTGA+TGG + chr2.4:6123534-6123553 None:intergenic 30.0%
!!! TTTTTTTTTTTGTGGTGGTC+GGG - chr2.4:6124235-6124254 MS.gene31110:intron 30.0%
AAAGAAGAGATTCAGAAGCA+AGG - chr2.4:6123351-6123370 MS.gene31110:intron 35.0%
AACCAAAAAGAAAGGTAACG+TGG - chr2.4:6123542-6123561 MS.gene31110:intron 35.0%
AGGATGAAGCAACAAATTCA+TGG + chr2.4:6125084-6125103 None:intergenic 35.0%
ATATGATTCTGCCTCAAGAA+TGG - chr2.4:6126407-6126426 MS.gene31110:CDS 35.0%
ATCCACCTATATGCTATTGA+GGG - chr2.4:6124055-6124074 MS.gene31110:intron 35.0%
ATTCCAACAGTCCAAATACT+CGG - chr2.4:6126169-6126188 MS.gene31110:intron 35.0%
CTTAAGTGTTACACTTTGTG+GGG + chr2.4:6125615-6125634 None:intergenic 35.0%
GCAACTACACTTTGACAAAA+CGG - chr2.4:6124692-6124711 MS.gene31110:intron 35.0%
GTCTTAAGTGTTACACTTTG+TGG + chr2.4:6125617-6125636 None:intergenic 35.0%
GTGCATAAGATATGCAAGAT+CGG + chr2.4:6124270-6124289 None:intergenic 35.0%
GTGGGATTTAATTAGTTCAC+CGG - chr2.4:6123023-6123042 MS.gene31110:intron 35.0%
TATCCACCTATATGCTATTG+AGG - chr2.4:6124054-6124073 MS.gene31110:intron 35.0%
TGATAGGTGAAATCCTAAAG+TGG - chr2.4:6124094-6124113 MS.gene31110:intron 35.0%
TGCATAAGATATGCAAGATC+GGG + chr2.4:6124269-6124288 None:intergenic 35.0%
TGTTACGCTTATAACAAGGT+AGG + chr2.4:6125592-6125611 None:intergenic 35.0%
TTACATCAAGCAGCAAAATG+AGG - chr2.4:6123326-6123345 MS.gene31110:intron 35.0%
TTAGGATTTCACCTATCACA+TGG + chr2.4:6124092-6124111 None:intergenic 35.0%
TTGCAATATCTTGGACTTAC+AGG + chr2.4:6125698-6125717 None:intergenic 35.0%
! ATCCACGTTACCTTTCTTTT+TGG + chr2.4:6123547-6123566 None:intergenic 35.0%
! TAGGTTTTTGTCTTCCAACA+AGG + chr2.4:6123129-6123148 None:intergenic 35.0%
! TGTTTTTCACTAGTCGGTAA+TGG - chr2.4:6123589-6123608 MS.gene31110:intron 35.0%
!! ATCAATCTAGTTGCCACTTT+AGG + chr2.4:6124110-6124129 None:intergenic 35.0%
!! ATCACACAAAGCATTGATTG+AGG - chr2.4:6123269-6123288 MS.gene31110:intron 35.0%
!!! CAGATCTTGTTTTGAACCAT+AGG - chr2.4:6126323-6126342 MS.gene31110:CDS 35.0%
!!! TGCTTTTTCTACCATGTGAT+AGG - chr2.4:6124078-6124097 MS.gene31110:intron 35.0%
!!! TTTTTTTTTTGTGGTGGTCG+GGG - chr2.4:6124236-6124255 MS.gene31110:intron 35.0%
AAGCACCCTCAATAGCATAT+AGG + chr2.4:6124063-6124082 None:intergenic 40.0%
AATCCCACGACTACATGAAA+AGG + chr2.4:6123011-6123030 None:intergenic 40.0%
ACACCTCACTGTTTGTACAA+TGG - chr2.4:6125495-6125514 MS.gene31110:intron 40.0%
ATACCGAGTATTTGGACTGT+TGG + chr2.4:6126175-6126194 None:intergenic 40.0%
ATGCCATTGTACAAACAGTG+AGG + chr2.4:6125501-6125520 None:intergenic 40.0%
ATGGCTAGATAGGCTAAATG+TGG + chr2.4:6124460-6124479 None:intergenic 40.0%
GAGATTCAGAAGCAAGGTAA+TGG - chr2.4:6123357-6123376 MS.gene31110:intron 40.0%
GAGGCAGAATCATATCATTC+TGG + chr2.4:6126402-6126421 None:intergenic 40.0%
GCATAAGATATGCAAGATCG+GGG + chr2.4:6124268-6124287 None:intergenic 40.0%
GCGACTTCTAAACAGAATCA+TGG - chr2.4:6123201-6123220 MS.gene31110:intron 40.0%
GGAAACATATGGTACCAGTT+CGG + chr2.4:6122901-6122920 None:intergenic 40.0%
GGCTAAATGTGGATGATTAG+TGG + chr2.4:6124449-6124468 None:intergenic 40.0%
GTGAAAACATACCTTCCCTA+TGG + chr2.4:6126342-6126361 None:intergenic 40.0%
TAGCAACAACAATCACACCA+TGG - chr2.4:6122758-6122777 MS.gene31110:CDS 40.0%
TGATTCTGATGGAGCAACAA+CGG + chr2.4:6123523-6123542 None:intergenic 40.0%
TGCTAACTGAGAGAACATCA+TGG + chr2.4:6122743-6122762 None:intergenic 40.0%
! AAAGAAGCGCGTAGATAGTA+AGG + chr2.4:6122923-6122942 None:intergenic 40.0%
! AAGAAGCGCGTAGATAGTAA+GGG + chr2.4:6122922-6122941 None:intergenic 40.0%
! ATTCAGAAGCAAGGTAATGG+AGG - chr2.4:6123360-6123379 MS.gene31110:intron 40.0%
! CTTCCTTTTCATGTAGTCGT+GGG - chr2.4:6123005-6123024 MS.gene31110:intron 40.0%
! GCTAGTTGATACCGAGTATT+TGG + chr2.4:6126183-6126202 None:intergenic 40.0%
! TATCTACGCGCTTCTTTTTC+AGG - chr2.4:6122926-6122945 MS.gene31110:intron 40.0%
! TCTTCCTTTTCATGTAGTCG+TGG - chr2.4:6123004-6123023 MS.gene31110:intron 40.0%
! TTTCACTAGTCGGTAATGGT+TGG - chr2.4:6123593-6123612 MS.gene31110:intron 40.0%
!! TTCAGAAGCAAGGTAATGGA+GGG - chr2.4:6123361-6123380 MS.gene31110:intron 40.0%
!!! CTTGTTTTGAACCATAGGGA+AGG - chr2.4:6126328-6126347 MS.gene31110:CDS 40.0%
AAAACGGCTCCCAAAGTTGT+TGG - chr2.4:6124708-6124727 MS.gene31110:intron 45.0%
ACCTACGCTAACGTGGTTAA+AGG - chr2.4:6123144-6123163 MS.gene31110:intron 45.0%
CAATCACACCATGGTTCCAA+CGG - chr2.4:6122767-6122786 MS.gene31110:CDS 45.0%
CACCCTCAATAGCATATAGG+TGG + chr2.4:6124060-6124079 None:intergenic 45.0%
CAGTTCGGAATTGTGACTCT+GGG + chr2.4:6122886-6122905 None:intergenic 45.0%
CCTACGCTAACGTGGTTAAA+GGG - chr2.4:6123145-6123164 MS.gene31110:intron 45.0%
CTACGCTAACGTGGTTAAAG+GGG - chr2.4:6123146-6123165 MS.gene31110:intron 45.0%
GACAAAAACCTACGCTAACG+TGG - chr2.4:6123137-6123156 MS.gene31110:intron 45.0%
GCACAACTCTTGCTGGTATA+AGG + chr2.4:6124665-6124684 None:intergenic 45.0%
TATACCAGCAAGAGTTGTGC+AGG - chr2.4:6124665-6124684 MS.gene31110:intron 45.0%
TCTTGCTGGTATAAGGACAG+TGG + chr2.4:6124658-6124677 None:intergenic 45.0%
TTAAGAATGGTACCACGGAG+TGG + chr2.4:6124741-6124760 None:intergenic 45.0%
! GGGGTGTTACGCTTATAACA+AGG + chr2.4:6125596-6125615 None:intergenic 45.0%
! TAGTCGGTAATGGTTGGTTG+CGG - chr2.4:6123599-6123618 MS.gene31110:intron 45.0%
! TGGTTGGTTGCGGAATCAAA+TGG - chr2.4:6123609-6123628 MS.gene31110:intron 45.0%
!!! ATGATGCAGGCCGTTTTTCT+TGG - chr2.4:6123447-6123466 MS.gene31110:intron 45.0%
!!! CTGTTGCTGCTTTTGTTGAC+TGG + chr2.4:6126375-6126394 None:intergenic 45.0%
!! TTAAAAAAAAAATACATTTA+AGG + chr2.4:6123916-6123935 None:intergenic 5.0%
!!! ATATCTTTAAAATTATTTAT+TGG + chr2.4:6123740-6123759 None:intergenic 5.0%
!!! TATCTTTAAAATTATTTATT+GGG + chr2.4:6123739-6123758 None:intergenic 5.0%
!!! TTTTTTTTTTAATTTAAATC+GGG + chr2.4:6125048-6125067 None:intergenic 5.0%
AAAGCCTGCACAACTCTTGC+TGG + chr2.4:6124672-6124691 None:intergenic 50.0%
ACCGGACCCACCAAGAAAAA+CGG + chr2.4:6123460-6123479 None:intergenic 50.0%
ACCTGTCCCACTTCTTGAAC+CGG + chr2.4:6123478-6123497 None:intergenic 50.0%
CACCGGTGAGTTGTAACCAA+GGG - chr2.4:6123061-6123080 MS.gene31110:intron 50.0%
CCAGAGTCACAATTCCGAAC+TGG - chr2.4:6122884-6122903 MS.gene31110:intron 50.0%
CCAGTTCGGAATTGTGACTC+TGG + chr2.4:6122887-6122906 None:intergenic 50.0%
CCCTTTAACCACGTTAGCGT+AGG + chr2.4:6123148-6123167 None:intergenic 50.0%
CTCCCTTGGTTACAACTCAC+CGG + chr2.4:6123066-6123085 None:intergenic 50.0%
GGATTCAACTCTGTGGTCAC+CGG - chr2.4:6123044-6123063 MS.gene31110:intron 50.0%
GGTGACCACAGAGTTGAATC+CGG + chr2.4:6123045-6123064 None:intergenic 50.0%
GTAACCAAGGGAGCTCAGAA+GGG - chr2.4:6123073-6123092 MS.gene31110:intron 50.0%
GTCCTCGTCCTCATCTAACA+AGG - chr2.4:6123394-6123413 MS.gene31110:intron 50.0%
GTCTTCCAACAAGGTGTAGC+TGG + chr2.4:6123120-6123139 None:intergenic 50.0%
GTGATGAAATCCTCGTCCGT+TGG + chr2.4:6122786-6122805 None:intergenic 50.0%
GTTCACCGGATTCAACTCTG+TGG - chr2.4:6123037-6123056 MS.gene31110:intron 50.0%
TCACCGGTGAGTTGTAACCA+AGG - chr2.4:6123060-6123079 MS.gene31110:intron 50.0%
TGAGCTTAGCTCAGCTGGTT+GGG + chr2.4:6124296-6124315 None:intergenic 50.0%
TGTAACCAAGGGAGCTCAGA+AGG - chr2.4:6123072-6123091 MS.gene31110:intron 50.0%
TTCCAACAAGGTGTAGCTGG+CGG + chr2.4:6123117-6123136 None:intergenic 50.0%
TTCGGAATTGTGACTCTGGG+AGG + chr2.4:6122883-6122902 None:intergenic 50.0%
!!! ATGCAGGCCGTTTTTCTTGG+TGG - chr2.4:6123450-6123469 MS.gene31110:intron 50.0%
!!! TGCAGGCCGTTTTTCTTGGT+GGG - chr2.4:6123451-6123470 MS.gene31110:intron 50.0%
AGAATGGTACCACGGAGTGG+AGG + chr2.4:6124738-6124757 None:intergenic 55.0%
ATCCTCGTCCGTTGGAACCA+TGG + chr2.4:6122778-6122797 None:intergenic 55.0%
ATGACCCTTCTGAGCTCCCT+TGG + chr2.4:6123080-6123099 None:intergenic 55.0%
CTGCATCATTCCTCCACCAC+TGG + chr2.4:6123436-6123455 None:intergenic 55.0%
GAATGGTACCACGGAGTGGA+GGG + chr2.4:6124737-6124756 None:intergenic 55.0%
GGAGGGACGCCAACAACTTT+GGG + chr2.4:6124720-6124739 None:intergenic 55.0%
GTGAGCTTAGCTCAGCTGGT+TGG + chr2.4:6124297-6124316 None:intergenic 55.0%
GTGGGTCCGGTTCAAGAAGT+GGG - chr2.4:6123469-6123488 MS.gene31110:intron 55.0%
TCCGGTTCAAGAAGTGGGAC+AGG - chr2.4:6123474-6123493 MS.gene31110:intron 55.0%
TGGAGGGACGCCAACAACTT+TGG + chr2.4:6124721-6124740 None:intergenic 55.0%
! CAGTGGTGGAGGAATGATGC+AGG - chr2.4:6123434-6123453 MS.gene31110:intron 55.0%
! GGTGTTTTCTTGCCTCGTGC+TGG - chr2.4:6123495-6123514 MS.gene31110:intron 55.0%
!! GGAGCAGGCCTTGTTAGATG+AGG + chr2.4:6123405-6123424 None:intergenic 55.0%
!! GGCCTTGTTAGATGAGGACG+AGG + chr2.4:6123399-6123418 None:intergenic 55.0%
!!! GCCGTTTTTCTTGGTGGGTC+CGG - chr2.4:6123456-6123475 MS.gene31110:intron 55.0%
CACCATGGTTCCAACGGACG+AGG - chr2.4:6122773-6122792 MS.gene31110:CDS 60.0%
CCAGCTGAGCTAAGCTCACG+AGG - chr2.4:6124298-6124317 MS.gene31110:intron 60.0%
CCATTGCAGACCAGTGGTGG+AGG - chr2.4:6123423-6123442 MS.gene31110:intron 60.0%
CCTCGTGAGCTTAGCTCAGC+TGG + chr2.4:6124301-6124320 None:intergenic 60.0%
CCTGCTCCATTGCAGACCAG+TGG - chr2.4:6123417-6123436 MS.gene31110:intron 60.0%
CTCCGCCAGCTACACCTTGT+TGG - chr2.4:6123112-6123131 MS.gene31110:intron 60.0%
GCAACAACGGTTCCAGCACG+AGG + chr2.4:6123510-6123529 None:intergenic 60.0%
GCTCCATTGCAGACCAGTGG+TGG - chr2.4:6123420-6123439 MS.gene31110:intron 60.0%
GGTGGGTCCGGTTCAAGAAG+TGG - chr2.4:6123468-6123487 MS.gene31110:intron 60.0%
! CCACTGGTCTGCAATGGAGC+AGG + chr2.4:6123420-6123439 None:intergenic 60.0%
! CCTCCACCACTGGTCTGCAA+TGG + chr2.4:6123426-6123445 None:intergenic 60.0%
GTTGGCGTCCCTCCACTCCG+TGG - chr2.4:6124726-6124745 MS.gene31110:intron 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 6122717 6126438 6122717 ID=MS.gene31110
chr2.4 mRNA 6122717 6126438 6122717 ID=MS.gene31110.t1;Parent=MS.gene31110
chr2.4 exon 6126250 6126438 6126250 ID=MS.gene31110.t1.exon1;Parent=MS.gene31110.t1
chr2.4 CDS 6126250 6126438 6126250 ID=cds.MS.gene31110.t1;Parent=MS.gene31110.t1
chr2.4 exon 6125918 6126134 6125918 ID=MS.gene31110.t1.exon2;Parent=MS.gene31110.t1
chr2.4 CDS 6125918 6126134 6125918 ID=cds.MS.gene31110.t1;Parent=MS.gene31110.t1
chr2.4 exon 6125600 6125875 6125600 ID=MS.gene31110.t1.exon3;Parent=MS.gene31110.t1
chr2.4 CDS 6125600 6125875 6125600 ID=cds.MS.gene31110.t1;Parent=MS.gene31110.t1
chr2.4 exon 6124408 6124512 6124408 ID=MS.gene31110.t1.exon4;Parent=MS.gene31110.t1
chr2.4 CDS 6124408 6124512 6124408 ID=cds.MS.gene31110.t1;Parent=MS.gene31110.t1
chr2.4 exon 6122717 6122829 6122717 ID=MS.gene31110.t1.exon5;Parent=MS.gene31110.t1
chr2.4 CDS 6122717 6122829 6122717 ID=cds.MS.gene31110.t1;Parent=MS.gene31110.t1
Gene Sequence

>MS.gene31110

ATGGCTGATGACAATAATATGTACCATGATGTTCTCTCAGTTAGCAACAACAATCACACCATGGTTCCAACGGACGAGGATTTCATCACCGAGTTAACTCATCAAATGTCTCAATTCATGCTTCAAGAAGATATTGACGACTCGTTCGATTTCTCATCTATTGCCTCCCAGAGTCACAATTCCGAACTGGTACCATATGTTTCCCTTACTATCTACGCGCTTCTTTTTCAGGTTTATTGAATAACTGATGTATCTGACCTATATTATTAACTCTTCTTTGTTTTCTTTCTTCCTTTTCATGTAGTCGTGGGATTTAATTAGTTCACCGGATTCAACTCTGTGGTCACCGGTGAGTTGTAACCAAGGGAGCTCAGAAGGGTCATCGCAGAACTCATCTCCGCCAGCTACACCTTGTTGGAAGACAAAAACCTACGCTAACGTGGTTAAAGGGGTTGAGAAAAATACTAAGAAGCTTAAAGAAACAGCGACTTCTAAACAGAATCATGGTTCTGATATTCAAAGTTTCAAGTCCTCCAATATTGATAGATTTTCATCACACAAAGCATTGATTGAGGAACAAATTAAAGCCATTGAATTAAGGAAAAAACATTACATCAAGCAGCAAAATGAGGCTAAAGAAGAGATTCAGAAGCAAGGTAATGGAGGGAACTGTACTCGTCCTCGTCCTCATCTAACAAGGCCTGCTCCATTGCAGACCAGTGGTGGAGGAATGATGCAGGCCGTTTTTCTTGGTGGGTCCGGTTCAAGAAGTGGGACAGGTGTTTTCTTGCCTCGTGCTGGAACCGTTGTTGCTCCATCAGAATCAACCAAAAAGAAAGGTAACGTGGATTATGTTATATCTATTCTGTTCATGTTTTTCACTAGTCGGTAATGGTTGGTTGCGGAATCAAATGGAATGTCTAGTTTCCCACTTTTAATATTCGAATCTGTTTATGATATTTTGTCTCTAAAATTCCACTCTTTTTCTACTTTCTAAGATTTCTTCTTGTTTGTTTCAACCCAATAAATAATTTTAAAGATATTTACATGGTTTTTAGTTAAGATTTATGAGCAATCAATCATTTCATTTTAAATGTGTGTGATGTGCTGTTATTATAATCCTCAAGTGTCTTCTTTATTAGTTATTGTTAGTTATTGTGATCACTAAATATGCTTCTTGTGTAATTTAACTTAATCCTTAAATGTATTTTTTTTTTAATCAAGTTAGTTCAAAAGAAACACTAGAAATATGTTTGCAATTTTAATAGATTTAAAATGGATCTCCCATTTGTAACTCTACAAGCTTAGTCCTCCAATTTTAAAAACATCAAATATTTTATCCACCTATATGCTATTGAGGGTGCTTTTTCTACCATGTGATAGGTGAAATCCTAAAGTGGCAACTAGATTGATGAGATGAAAATGAGTTATCTAACTAGATTGATGCTATTAAGGGTGCATTAAATGTAATAACTCTTAAGATATAAGTTAAATGTATGTTTTGAGAAAATGAAAGCTTTTTTTTTTTTGTGGTGGTCGGGGTTTGAACCCCGATCTTGCATATCTTATGCACTGTCCCAACCAGCTGAGCTAAGCTCACGAGGACATAGAAAATGAAAATTCAAGTGATAATCCATCTAAATATGTCATGTCATCCACTAAGTTGTCACATAAACATGTAGATTTTTTTTTTTAAAAAAATATTCTTAATATTACGCTTCATTAATATCCACTAATCATCCACATTTAGCCTATCTAGCCATCAAAAAATTAAGCAGTTAGAAAGTTTGGTGAGCAAAATTGAAGATTCGTTAATGTATGAGTGAATCCTTAATTAATTATACTTAACTGCCAAAACAATATCAAATTCGTTTCACATCTTACATCAAAATTGATTTGTTTTATGCACGTTATATATATTGTTAGGGAAAGGTTGCTCCACTGTCCTTATACCAGCAAGAGTTGTGCAGGCTTTGCAACTACACTTTGACAAAACGGCTCCCAAAGTTGTTGGCGTCCCTCCACTCCGTGGTACCATTCTTAATTTATTATTGTTAATTATACCATCAAGCAACAACTTTGATTTGTTGTTTATTTATTGCTATTCTTCATGCCGTTTAGTTACTACTCTCCTAATAAATAAATAATCTTACCCAACAAAATATGTGGATGAGATGATAAGAATCTCTTCCAAGTTAACTAAAAGTCTTAGGTTCGAATCAAACTCTAAAAATACAACAATGTTAAATCTTTTTAAGGAGAATTTTTTTACCATTGTAATCCTAATCTATTCGAGATGTTAGTTTGTGCAATTATTCGTATAGGACACCCGATTTAAATTAAAAAAAAAAACAAAACTCATGACCATGAATTTGTTGCTTCATCCTTAGCAACATGACATTCAATAAACTTTTAGTTGTTATATCAATTCCCATTTTATATGCATATAAAATTTATTTGGGTAATTCCTAAGTTTAATGCTCTAAGTTTGGTTGTATTCAATATAGTCTATCAATTTAAATGATTATCCTTTCTTTTTAAAATTATTTGGGCAATTCCTAAGTTTTATACTCTAAGTTTTGTAGCACCAAAAATAAAATACGCTTAAGTTTTGTAGAAAACAAAAAAACTCTAAGTTTGCTACTGACTTTCCTATTTCATTTTCTTATCAATCATATAAATTTATAAACAATTTCTACATGGAAAATTCTACGGTGAAGTCATGAGAATGACTATTTTGGTGAAGTTAACACTATAACATCAAAAATGTAATGAGTAACACCTCACTGTTTGTACAATGGCATCAAAAGTTCAGTCCTCATAGTATCATCAATTCATCAGATTAACAAGATTACACTTAATCTAATTTTATCCTACCTTGTTATAAGCGTAACACCCCACAAAGTGTAACACTTAAGACTATCACATAATATCATCAACTCTGCTTGCATGTTAAAGATTCCGACAATAAAATAGTACCTGTAAGTCCAAGATATTGCAAGCATTACTAGATGAATTACTATTGATCATTAATCAGTAATCAACAATTTAAAAATGAATCAAACATTAAGAACAAAATTAACTTTTTCAAAAAAGTCAGTTGTCTGACTGCACTATAGAAGCTCCCTTTCTACATTTGTTTAATTTATTCACTCTTTGATTTCCTATCAATTTCTCATCTGTTTGCTTACACTTAAGTCTTGCGCAATGTTAGATTGTATTAAATGTCACACTCACACACACATCATCTAGTTGTTTTTTTATACTTTTCTTTAGAAAAATTGGCATACGTCATGTTTAAAAAAAACAAAAATTATGAATAAATTAAAATTAGTATATTCTATGAAAGACTTCTAGTTAATACTAATCTCTCTATGATTACTTTGATTTGCAATTCTTAATAATGGAATGTATTAGCTGTCCAATTTTATTTTTCGAGACTGCTGCAATTTATTCCAACAGTCCAAATACTCGGTATCAACTAGCAATTTGTCGCCAACTAGTTCTATAATATAAAAAAAACACTGTCTGATTTGATTTTCATCTATTTTTTATTTATTTGTTGTTAATCAATTTGCTCTATAATATTGTTATTATTATGAATTGCAGATCTTGTTTTGAACCATAGGGAAGGTATGTTTTCACTTGAGAATCACCAGTCAACAAAAGCAGCAACAGATGTCCAGAATGATATGATTCTGCCTCAAGAATGGACATATTGA
Protein sequence

>MS.gene31110.t1

MADDNNMYHDVLSVSNNNHTMVPTDEDFITELTHQMSQFMLQEDIDDSFDFSSIASQSHNSELSWDLISSPDSTLWSPVSCNQGSSEGSSQNSSPPATPCWKTKTYANVVKGVEKNTKKLKETATSKQNHGSDIQTLIEEQIKAIELRKKHYIKQQNEAKEEIQKQGNGGNCTRPRPHLTRPAPLQTSGGGMMQAVFLGGSGSRSGTGVFLPRAGTVVAPSESTKKKGKGCSTVLIPARVVQALQLHFDKTAPKVVGVPPLRDLVLNHREGMFSLENHQSTKAATDVQNDMILPQEWTY