AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052071

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052071.t1 MTR_2g097800 84.308 325 21 6 1 299 1 321 0.0 519
MS.gene052071.t1 MTR_4g064947 38.462 260 127 7 73 299 118 377 5.21e-45 157
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052071.t1 AT5G59050 36.129 310 114 18 25 299 52 312 1.06e-21 93.2
MS.gene052071.t1 AT5G59050 38.000 200 64 13 135 299 17 191 7.31e-11 60.8

Find 70 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTACGCTAACGTGGTTAA+AGG 0.245389 2.2:-4768717 MS.gene052071:CDS
AAATTAAAGCCATTGAATTA+AGG 0.289984 2.2:-4768566 MS.gene052071:CDS
GCACAACTCTTGCTGGTATA+AGG 0.306651 2.2:+4766934 None:intergenic
CCTACGCTAACGTGGTTAAA+GGG 0.390281 2.2:-4768716 MS.gene052071:CDS
AAAGCCTGCACAACTCTTGC+TGG 0.391708 2.2:+4766927 None:intergenic
ACCTGTCCCACTTCTTGAAC+CGG 0.402912 2.2:+4768386 None:intergenic
CTCCGCCAGCTACACCTTGT+TGG 0.404982 2.2:-4768749 MS.gene052071:CDS
GGAGGGACGCCAACAACTTT+GGG 0.411943 2.2:+4766879 None:intergenic
GAGGCAGAATCATATCATTC+TGG 0.418235 2.2:+4764946 None:intergenic
GTCTTCCAACAAGGTGTAGC+TGG 0.435910 2.2:+4768744 None:intergenic
TGGAGGGACGCCAACAACTT+TGG 0.438518 2.2:+4766878 None:intergenic
GAGATTCAGAAGCAAGGTAA+TGG 0.461458 2.2:-4768504 MS.gene052071:CDS
GCAACTACACTTTGACAAAA+CGG 0.470788 2.2:-4766904 MS.gene052071:CDS
CCACTGGTCTGCAATGGAGC+AGG 0.476520 2.2:+4768444 None:intergenic
CCAGTTCGGAATTGTGACTC+TGG 0.478083 2.2:+4768977 None:intergenic
ATATGATTCTGCCTCAAGAA+TGG 0.490516 2.2:-4764938 MS.gene052071:CDS
ATGACCCTTCTGAGCTCCCT+TGG 0.499706 2.2:+4768784 None:intergenic
TATACCAGCAAGAGTTGTGC+AGG 0.500359 2.2:-4766931 MS.gene052071:CDS
GCTCCATTGCAGACCAGTGG+TGG 0.500459 2.2:-4768441 MS.gene052071:CDS
CTGCATCATTCCTCCACCAC+TGG 0.505752 2.2:+4768428 None:intergenic
GTGGGATTTAATTAGTTCAC+CGG 0.508002 2.2:-4768838 MS.gene052071:CDS
TGTAACCAAGGGAGCTCAGA+AGG 0.515050 2.2:-4768789 MS.gene052071:CDS
CCAGAGTCACAATTCCGAAC+TGG 0.519755 2.2:-4768977 MS.gene052071:intron
TCCGGTTCAAGAAGTGGGAC+AGG 0.522917 2.2:-4768387 MS.gene052071:CDS
CCTCCACCACTGGTCTGCAA+TGG 0.524420 2.2:+4768438 None:intergenic
ATCACACAAAGCATTGATTG+AGG 0.524445 2.2:-4768592 MS.gene052071:intron
CCATTGCAGACCAGTGGTGG+AGG 0.531905 2.2:-4768438 MS.gene052071:CDS
AAAACGGCTCCCAAAGTTGT+TGG 0.534159 2.2:-4766888 MS.gene052071:CDS
GGTGGGTCCGGTTCAAGAAG+TGG 0.535246 2.2:-4768393 MS.gene052071:CDS
GTGAAAACATACCTTCCCTA+TGG 0.540528 2.2:+4765006 None:intergenic
ATTCAGAAGCAAGGTAATGG+AGG 0.545729 2.2:-4768501 MS.gene052071:CDS
CACCATGGTTCCAACGGACG+AGG 0.546751 2.2:-4769088 MS.gene052071:CDS
GGAAACATATGGTACCAGTT+CGG 0.547360 2.2:+4768963 None:intergenic
ATCCTCGTCCGTTGGAACCA+TGG 0.552840 2.2:+4769086 None:intergenic
CTCCCTTGGTTACAACTCAC+CGG 0.562393 2.2:+4768798 None:intergenic
CAGTTCGGAATTGTGACTCT+GGG 0.562437 2.2:+4768978 None:intergenic
CAGTGGTGGAGGAATGATGC+AGG 0.565231 2.2:-4768427 MS.gene052071:CDS
GCGACTTCTAAACAGAATCA+TGG 0.566085 2.2:-4768660 MS.gene052071:CDS
GAATGGTACCACGGAGTGGA+GGG 0.567764 2.2:+4766862 None:intergenic
CTTCAATATGTCCATTCTTG+AGG 0.572811 2.2:+4764927 None:intergenic
CAATCACACCATGGTTCCAA+CGG 0.579340 2.2:-4769094 MS.gene052071:CDS
GGATTCAACTCTGTGGTCAC+CGG 0.580045 2.2:-4768817 MS.gene052071:CDS
GTGATGAAATCCTCGTCCGT+TGG 0.582382 2.2:+4769078 None:intergenic
TTACATCAAGCAGCAAAATG+AGG 0.589469 2.2:-4768535 MS.gene052071:CDS
GGTGACCACAGAGTTGAATC+CGG 0.590456 2.2:+4768819 None:intergenic
CTACGCTAACGTGGTTAAAG+GGG 0.595945 2.2:-4768715 MS.gene052071:CDS
GTGGGTCCGGTTCAAGAAGT+GGG 0.596347 2.2:-4768392 MS.gene052071:CDS
AGAATGGTACCACGGAGTGG+AGG 0.600926 2.2:+4766861 None:intergenic
CCCTTTAACCACGTTAGCGT+AGG 0.602141 2.2:+4768716 None:intergenic
AAAGAAGAGATTCAGAAGCA+AGG 0.613101 2.2:-4768510 MS.gene052071:CDS
AGACATTTGATGAGTTAACT+CGG 0.619910 2.2:+4769056 None:intergenic
TTCAGAAGCAAGGTAATGGA+GGG 0.622585 2.2:-4768500 MS.gene052071:CDS
TGATTCTGATGGAGCAACAA+CGG 0.623497 2.2:+4768341 None:intergenic
GGCCTTGTTAGATGAGGACG+AGG 0.624372 2.2:+4768465 None:intergenic
CCTGCTCCATTGCAGACCAG+TGG 0.625104 2.2:-4768444 MS.gene052071:CDS
CACCGGTGAGTTGTAACCAA+GGG 0.632229 2.2:-4768800 MS.gene052071:CDS
GCAACAACGGTTCCAGCACG+AGG 0.632333 2.2:+4768354 None:intergenic
TTCGGAATTGTGACTCTGGG+AGG 0.633765 2.2:+4768981 None:intergenic
TTCCAACAAGGTGTAGCTGG+CGG 0.645177 2.2:+4768747 None:intergenic
GGAGCAGGCCTTGTTAGATG+AGG 0.651849 2.2:+4768459 None:intergenic
GTCCTCGTCCTCATCTAACA+AGG 0.653193 2.2:-4768467 MS.gene052071:CDS
TTAAGAATGGTACCACGGAG+TGG 0.655116 2.2:+4766858 None:intergenic
GTAACCAAGGGAGCTCAGAA+GGG 0.662619 2.2:-4768788 MS.gene052071:CDS
TCTTGCTGGTATAAGGACAG+TGG 0.678497 2.2:+4766941 None:intergenic
GTTGGCGTCCCTCCACTCCG+TGG 0.686225 2.2:-4766870 MS.gene052071:intron
GATGATAATAATATGTACCA+TGG 0.688154 2.2:-4769138 MS.gene052071:CDS
TCACCGGTGAGTTGTAACCA+AGG 0.715334 2.2:-4768801 MS.gene052071:CDS
GTTCACCGGATTCAACTCTG+TGG 0.734801 2.2:-4768824 MS.gene052071:CDS
TAGCAACAACAATCACACCA+TGG 0.737768 2.2:-4769103 MS.gene052071:CDS
TGCTAAGTGAGAGAACACCA+TGG 0.818904 2.2:+4769121 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTAAATTTAAAT+CGG + chr2.2:4767527-4767546 None:intergenic 0.0%
!! ATATGCATATAAAATTTATT+TGG - chr2.2:4767631-4767650 MS.gene052071:intron 10.0%
!! ATTTAGTATTATATTGTATT+GGG + chr2.2:4768763-4768782 None:intergenic 10.0%
!! TAACAATAATAAATTAAGAA+TGG + chr2.2:4767231-4767250 None:intergenic 10.0%
!! TATGCATATAAAATTTATTT+GGG - chr2.2:4767632-4767651 MS.gene052071:intron 10.0%
!! TATTTAGTATTATATTGTAT+TGG + chr2.2:4768764-4768783 None:intergenic 10.0%
!!! AACAATATTAAATCTTTTTA+AGG - chr2.2:4767431-4767450 MS.gene052071:intron 10.0%
!!! AATTTTATATGCATATAAAA+TGG + chr2.2:4767627-4767646 None:intergenic 10.0%
!!! ATTTTATATGCATATAAAAT+GGG + chr2.2:4767645-4767626 None:intergenic 10.0%
!!! CAATTTTAATAGATTTAAAA+TGG - chr2.2:4766180-4766199 MS.gene052071:intron 10.0%
!!! CTTTCTTTTTAAAATTATTT+GGG - chr2.2:4767722-4767741 MS.gene052071:intron 10.0%
!!! TAATTTTAAAGATATTTACA+TGG - chr2.2:4765962-4765981 MS.gene052071:intron 10.0%
!!! TTTTTTTTTTTTTTTAAGGA+AGG + chr2.2:4766757-4766776 None:intergenic 10.0%
!! AAATTAAACAAATGTAGAAA+GGG + chr2.2:4768318-4768337 None:intergenic 15.0%
!! CAGTTAAGTATAATTAATTA+AGG + chr2.2:4767022-4767041 None:intergenic 15.0%
!! GGTAAGATTATTTATTTATT+AGG + chr2.2:4767328-4767347 None:intergenic 15.0%
!! TAAATTAAACAAATGTAGAA+AGG + chr2.2:4768319-4768338 None:intergenic 15.0%
!!! AAGAAAAGTGTATAAATAAA+AGG + chr2.2:4768828-4768847 None:intergenic 15.0%
!!! ACTTAAGAGTATTTTATTTT+TGG + chr2.2:4767784-4767803 None:intergenic 15.0%
!!! AGAAAAGTGTATAAATAAAA+GGG + chr2.2:4768827-4768846 None:intergenic 15.0%
!!! CCAAATAATTTTAAAAAGAA+AGG + chr2.2:4767724-4767743 None:intergenic 15.0%
!!! CCTTTCTTTTTAAAATTATT+TGG - chr2.2:4767721-4767740 MS.gene052071:intron 15.0%
!!! TATACTTTCTTTAGAAAAAT+TGG - chr2.2:4768452-4768471 MS.gene052071:CDS 15.0%
!!! TATCATTTCTCTTTTAAAAT+TGG + chr2.2:4766239-4766258 None:intergenic 15.0%
!! AAAGTGGAATATATTGTATT+GGG + chr2.2:4768804-4768823 None:intergenic 20.0%
!! AAATTAAAGCCATTGAATTA+AGG - chr2.2:4765507-4765526 MS.gene052071:intron 20.0%
!! CAAAGAAGAGTTAATAATAT+AGG + chr2.2:4765189-4765208 None:intergenic 20.0%
!! GTTGTATTGAAAATTGAAAT+TGG + chr2.2:4768730-4768749 None:intergenic 20.0%
!! TGATGAAAATCTATCAATAT+TGG + chr2.2:4765465-4765484 None:intergenic 20.0%
!! TTATATATATTGTTAGGGAA+AGG - chr2.2:4767104-4767123 MS.gene052071:intron 20.0%
!! TTTCTACATGAAAAATTCTA+TGG - chr2.2:4767884-4767903 MS.gene052071:intron 20.0%
!!! AACTTAAGAGTATAAAACTT+AGG + chr2.2:4767753-4767772 None:intergenic 20.0%
!!! AGACACTTTTTTTTAGAAAA+TGG + chr2.2:4768695-4768714 None:intergenic 20.0%
!!! ATGTTTTTTCCTTAATTCAA+TGG + chr2.2:4765519-4765538 None:intergenic 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG + chr2.2:4768694-4768713 None:intergenic 20.0%
!!! TGATTTGCAATTCTTAATAA+TGG - chr2.2:4768574-4768593 MS.gene052071:CDS 20.0%
! AAAAATAAAACAGAACCGAA+AGG + chr2.2:4766920-4766939 None:intergenic 25.0%
! AACTAGATTGATGCTATTAA+GGG - chr2.2:4766526-4766545 MS.gene052071:intron 25.0%
! AATAAGAAGTGAGAAAAACA+AGG - chr2.2:4766363-4766382 MS.gene052071:intron 25.0%
! AATTAAGCAGTTAGAAAGTT+TGG - chr2.2:4766962-4766981 MS.gene052071:intron 25.0%
! ACAGATTCGAATATTAAAAG+TGG + chr2.2:4765860-4765879 None:intergenic 25.0%
! ATAAATTAAGAATGGTACCA+CGG + chr2.2:4767223-4767242 None:intergenic 25.0%
! ATAGATTCGTTAATGTTTGA+AGG - chr2.2:4766993-4767012 MS.gene052071:intron 25.0%
! ATCTTACCCAACAAAATATT+TGG - chr2.2:4767340-4767359 MS.gene052071:intron 25.0%
! CAGATTCGAATATTAAAAGT+GGG + chr2.2:4765859-4765878 None:intergenic 25.0%
! GAAAGTGGAATATATTGTAT+TGG + chr2.2:4768805-4768824 None:intergenic 25.0%
! GATGATAATAATATGTACCA+TGG - chr2.2:4764935-4764954 MS.gene052071:CDS 25.0%
! GCACGTTATATATATTGTTA+GGG - chr2.2:4767099-4767118 MS.gene052071:intron 25.0%
! TAACTAGATTGATGCTATTA+AGG - chr2.2:4766525-4766544 MS.gene052071:intron 25.0%
! TGAAAATCTATCAATATTGG+AGG + chr2.2:4765462-4765481 None:intergenic 25.0%
! TGATATTATGTGATAGTCTT+AGG + chr2.2:4768107-4768126 None:intergenic 25.0%
! TGCACGTTATATATATTGTT+AGG - chr2.2:4767098-4767117 MS.gene052071:intron 25.0%
! TGTATAAATAAAAGGGAAAG+TGG + chr2.2:4768820-4768839 None:intergenic 25.0%
! TTCTTATTGGTCAAAAACAA+TGG + chr2.2:4766351-4766370 None:intergenic 25.0%
!! TACAGGTACTATTTTATTGT+CGG + chr2.2:4768156-4768175 None:intergenic 25.0%
!! TCTCATCCAAATATTTTGTT+GGG + chr2.2:4767349-4767368 None:intergenic 25.0%
!! TTCTCATCCAAATATTTTGT+TGG + chr2.2:4767350-4767369 None:intergenic 25.0%
!!! CATTTCTCTTTTAAAATTGG+AGG + chr2.2:4766236-4766255 None:intergenic 25.0%
!!! CGAATTTGATAGTATTGTTT+TGG + chr2.2:4767045-4767064 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG + chr2.2:4766364-4766383 None:intergenic 25.0%
AAAACAATGGAGAGAGAAAA+AGG + chr2.2:4766338-4766357 None:intergenic 30.0%
AAGCTTGTAGAGTTACAAAT+GGG + chr2.2:4766210-4766229 None:intergenic 30.0%
ACTAACATCTCGAATAGATT+AGG + chr2.2:4767479-4767498 None:intergenic 30.0%
AGACATTTGATGAGTTAACT+CGG + chr2.2:4765020-4765039 None:intergenic 30.0%
ATGTTTATGTGACAACTTAG+TGG + chr2.2:4766648-4766667 None:intergenic 30.0%
ATTTCACCTCAATAGCATAT+AGG + chr2.2:4766463-4766482 None:intergenic 30.0%
CAAACAGATGAGAAATTGAT+AGG + chr2.2:4768357-4768376 None:intergenic 30.0%
CAACCACCATAAAGAATATT+TGG + chr2.2:4766837-4766856 None:intergenic 30.0%
CAGCATGTTACTATAGATTA+AGG + chr2.2:4766872-4766891 None:intergenic 30.0%
CCAAGTTAACTAAAAGTCTT+AGG - chr2.2:4767384-4767403 MS.gene052071:intron 30.0%
CGAATAGATTAGGATTACAA+TGG + chr2.2:4767469-4767488 None:intergenic 30.0%
CTTAGAAAGTAGAAAAAGAG+TGG + chr2.2:4765907-4765926 None:intergenic 30.0%
GATGACATGACATATTTAGA+TGG + chr2.2:4766626-4766645 None:intergenic 30.0%
GCAGTCTCGAAAAATAAAAT+TGG + chr2.2:4768614-4768633 None:intergenic 30.0%
GTTTGTGCAATTATTCGTAT+AGG - chr2.2:4767497-4767516 MS.gene052071:intron 30.0%
TAAGCTTGTAGAGTTACAAA+TGG + chr2.2:4766211-4766230 None:intergenic 30.0%
TAGATAGTAAGGGAAACATA+TGG + chr2.2:4765124-4765143 None:intergenic 30.0%
TATTGAATGTCATGTTGCTA+AGG + chr2.2:4767580-4767599 None:intergenic 30.0%
TCAACAAAGTTGTCATCAAA+TGG - chr2.2:4766389-4766408 MS.gene052071:intron 30.0%
TCAGAATCAACCAAAAAGAA+AGG - chr2.2:4765746-4765765 MS.gene052071:intron 30.0%
TGACCAAATATTCTTTATGG+TGG - chr2.2:4766831-4766850 MS.gene052071:intron 30.0%
TGGTGACCAAATATTCTTTA+TGG - chr2.2:4766828-4766847 MS.gene052071:intron 30.0%
TTAGGAGAGTAGTAACTAAA+CGG + chr2.2:4767310-4767329 None:intergenic 30.0%
! AAACTTAGAGCGTTAAACTT+AGG + chr2.2:4767663-4767682 None:intergenic 30.0%
! AAATCAAAGTTGTTGCTTGA+TGG + chr2.2:4767260-4767279 None:intergenic 30.0%
! AGTCATGAGAATGACTATTT+TGG - chr2.2:4767910-4767929 MS.gene052071:intron 30.0%
! GATGAATTGATGATACTATG+AGG + chr2.2:4768011-4768030 None:intergenic 30.0%
! TAAGTTTAACGCTCTAAGTT+TGG - chr2.2:4767662-4767681 MS.gene052071:intron 30.0%
! TGTTCATGTTTTTCACTAGT+CGG - chr2.2:4765795-4765814 MS.gene052071:intron 30.0%
!! AGTTGATGATTTGTTCCTTT+CGG - chr2.2:4766902-4766921 MS.gene052071:CDS 30.0%
!! CCTAAGACTTTTAGTTAACT+TGG + chr2.2:4767387-4767406 None:intergenic 30.0%
!!! AACCACTTTTTGACAACTTT+TGG - chr2.2:4766266-4766285 MS.gene052071:intron 30.0%
!!! AGATCTTGTTTTGAACCATA+GGG - chr2.2:4769052-4769071 MS.gene052071:CDS 30.0%
!!! TTCTTTTTGGTTGATTCTGA+TGG + chr2.2:4765746-4765765 None:intergenic 30.0%
AAAGAAGAGATTCAGAAGCA+AGG - chr2.2:4765563-4765582 MS.gene052071:intron 35.0%
AACCAAAAAGAAAGGTAACG+TGG - chr2.2:4765754-4765773 MS.gene052071:intron 35.0%
AGAAAATGGGTCAGTTATTC+CGG + chr2.2:4768681-4768700 None:intergenic 35.0%
AGGATGAAGCAACAAATTCA+TGG + chr2.2:4767560-4767579 None:intergenic 35.0%
AGTACAAAGGCAAGATAGAT+AGG + chr2.2:4766783-4766802 None:intergenic 35.0%
ATATGATTCTGCCTCAAGAA+TGG - chr2.2:4769135-4769154 MS.gene052071:CDS 35.0%
ATGTCATTGTACAAACAGTG+AGG + chr2.2:4767976-4767995 None:intergenic 35.0%
ATTGAGGTGAAATCCTAAAG+TGG - chr2.2:4766470-4766489 MS.gene052071:intron 35.0%
CCAAAGTAGGTCAATTATAC+CGG - chr2.2:4768863-4768882 MS.gene052071:intron 35.0%
GCAACTACACTTTGACAAAA+CGG - chr2.2:4767169-4767188 MS.gene052071:intron 35.0%
GTCCAAAAGTTGTCAAAAAG+TGG + chr2.2:4766271-4766290 None:intergenic 35.0%
GTGGGATTTAATTAGTTCAC+CGG - chr2.2:4765235-4765254 MS.gene052071:intron 35.0%
TAATTAAATCCCACGACTAC+AGG + chr2.2:4765229-4765248 None:intergenic 35.0%
TAGTAATGCTTGCAACATCT+TGG + chr2.2:4768182-4768201 None:intergenic 35.0%
TATCCACCTATATGCTATTG+AGG - chr2.2:4766454-4766473 MS.gene052071:intron 35.0%
TCACATGCAAACTAGTACAA+AGG + chr2.2:4766796-4766815 None:intergenic 35.0%
TCTGTGTGAAAAACCAAAGT+AGG - chr2.2:4768850-4768869 MS.gene052071:intron 35.0%
TCTTACGATATAAGCTCATG+AGG - chr2.2:4766567-4766586 MS.gene052071:intron 35.0%
TGTTACGCTTATAACAAGGT+AGG + chr2.2:4768067-4768086 None:intergenic 35.0%
TTACATCAAGCAGCAAAATG+AGG - chr2.2:4765538-4765557 MS.gene052071:intron 35.0%
TTGCAACATCTTGGATTTAC+AGG + chr2.2:4768173-4768192 None:intergenic 35.0%
! ATCCACGTTACCTTTCTTTT+TGG + chr2.2:4765759-4765778 None:intergenic 35.0%
! TAGGTTTTTGTCTTCCAACA+AGG + chr2.2:4765341-4765360 None:intergenic 35.0%
! TCTTAGGTGTTACACTTTGT+GGG + chr2.2:4768091-4768110 None:intergenic 35.0%
! TGTACTAGTTTGCATGTGAT+CGG - chr2.2:4766797-4766816 MS.gene052071:intron 35.0%
! TGTTTTTCACTAGTCGGTAA+TGG - chr2.2:4765801-4765820 MS.gene052071:intron 35.0%
!! ATCAATCTAGTTGCCACTTT+AGG + chr2.2:4766486-4766505 None:intergenic 35.0%
!! ATCACACAAAGCATTGATTG+AGG - chr2.2:4765481-4765500 MS.gene052071:intron 35.0%
!!! CAGATCTTGTTTTGAACCAT+AGG - chr2.2:4769051-4769070 MS.gene052071:CDS 35.0%
CCGGTATAATTGACCTACTT+TGG + chr2.2:4768866-4768885 None:intergenic 40.0%
CTAGCTAGATAGGCTAAATG+TGG + chr2.2:4766732-4766751 None:intergenic 40.0%
GAAAATGGGTCAGTTATTCC+GGG + chr2.2:4768680-4768699 None:intergenic 40.0%
GAGATTCAGAAGCAAGGTAA+TGG - chr2.2:4765569-4765588 MS.gene052071:intron 40.0%
GAGGCAGAATCATATCATTC+TGG + chr2.2:4769130-4769149 None:intergenic 40.0%
GCGACTTCTAAACAGAATCA+TGG - chr2.2:4765413-4765432 MS.gene052071:intron 40.0%
GGAAACATATGGTACCAGTT+CGG + chr2.2:4765113-4765132 None:intergenic 40.0%
GGCTAAATGTGGATGATTAG+TGG + chr2.2:4766721-4766740 None:intergenic 40.0%
GTAGGTCAATTATACCGGAA+CGG - chr2.2:4768868-4768887 MS.gene052071:intron 40.0%
GTGAAAACATACCTTCCCTA+TGG + chr2.2:4769070-4769089 None:intergenic 40.0%
TAGCAACAACAATCACACCA+TGG - chr2.2:4764970-4764989 MS.gene052071:CDS 40.0%
TCACCTCAATAGCATATAGG+TGG + chr2.2:4766460-4766479 None:intergenic 40.0%
TGATTCTGATGGAGCAACAA+CGG + chr2.2:4765735-4765754 None:intergenic 40.0%
! AAAGAAGCGCGTAGATAGTA+AGG + chr2.2:4765135-4765154 None:intergenic 40.0%
! AAGAAGCGCGTAGATAGTAA+GGG + chr2.2:4765134-4765153 None:intergenic 40.0%
! ATTCAGAAGCAAGGTAATGG+AGG - chr2.2:4765572-4765591 MS.gene052071:intron 40.0%
! CTTAGGTGTTACACTTTGTG+GGG + chr2.2:4768090-4768109 None:intergenic 40.0%
! GTCTTAGGTGTTACACTTTG+TGG + chr2.2:4768092-4768111 None:intergenic 40.0%
! TATCTACGCGCTTCTTTTTC+AGG - chr2.2:4765138-4765157 MS.gene052071:intron 40.0%
! TTTCACTAGTCGGTAATGGT+TGG - chr2.2:4765805-4765824 MS.gene052071:intron 40.0%
!! TTCAGAAGCAAGGTAATGGA+GGG - chr2.2:4765573-4765592 MS.gene052071:intron 40.0%
!!! CTTGTTTTGAACCATAGGGA+AGG - chr2.2:4769056-4769075 MS.gene052071:CDS 40.0%
AAAACGGCTCCCAAAGTTGT+TGG - chr2.2:4767185-4767204 MS.gene052071:intron 45.0%
AAGGAAGGAGCTAGCTAGAT+AGG + chr2.2:4766742-4766761 None:intergenic 45.0%
AATCCCACGACTACAGGAAA+AGG + chr2.2:4765223-4765242 None:intergenic 45.0%
ACCTACGCTAACGTGGTTAA+AGG - chr2.2:4765356-4765375 MS.gene052071:intron 45.0%
CAATCACACCATGGTTCCAA+CGG - chr2.2:4764979-4764998 MS.gene052071:CDS 45.0%
CAGTTCGGAATTGTGACTCT+GGG + chr2.2:4765098-4765117 None:intergenic 45.0%
CCTACGCTAACGTGGTTAAA+GGG - chr2.2:4765357-4765376 MS.gene052071:intron 45.0%
CTACGCTAACGTGGTTAAAG+GGG - chr2.2:4765358-4765377 MS.gene052071:intron 45.0%
GACAAAAACCTACGCTAACG+TGG - chr2.2:4765349-4765368 MS.gene052071:intron 45.0%
GCACAACTCTTGCTGGTATA+AGG + chr2.2:4767142-4767161 None:intergenic 45.0%
GTACTGAGTATTCGGACTGT+TGG + chr2.2:4768648-4768667 None:intergenic 45.0%
TATACCAGCAAGAGTTGTGC+AGG - chr2.2:4767142-4767161 MS.gene052071:intron 45.0%
TCTTGCTGGTATAAGGACAG+TGG + chr2.2:4767135-4767154 None:intergenic 45.0%
TGCTAAGTGAGAGAACACCA+TGG + chr2.2:4764955-4764974 None:intergenic 45.0%
TTAAGAATGGTACCACGGAG+TGG + chr2.2:4767218-4767237 None:intergenic 45.0%
! AGTTGATACTACCTCCGTTC+CGG + chr2.2:4768885-4768904 None:intergenic 45.0%
! CTAGTTTGCATGTGATCGGA+TGG - chr2.2:4766801-4766820 MS.gene052071:intron 45.0%
! CTTCCTTTTCCTGTAGTCGT+GGG - chr2.2:4765217-4765236 MS.gene052071:intron 45.0%
! GGGGTGTTACGCTTATAACA+AGG + chr2.2:4768071-4768090 None:intergenic 45.0%
! TAGTCGGTAATGGTTGGTTG+CGG - chr2.2:4765811-4765830 MS.gene052071:intron 45.0%
! TCTTCCTTTTCCTGTAGTCG+TGG - chr2.2:4765216-4765235 MS.gene052071:intron 45.0%
! TGGTTGGTTGCGGAATCAAA+TGG - chr2.2:4765821-4765840 MS.gene052071:intron 45.0%
!!! ATGATGCAGGCCGTTTTTCT+TGG - chr2.2:4765659-4765678 MS.gene052071:intron 45.0%
!!! CTGTTGCTGCTTTTGTTGAC+TGG + chr2.2:4769103-4769122 None:intergenic 45.0%
!! TAAAAAAAAAAATACATTTA+AGG + chr2.2:4766123-4766142 None:intergenic 5.0%
!!! ATATCTTTAAAATTATTTAT+TGG + chr2.2:4765957-4765976 None:intergenic 5.0%
!!! GTTTTTTTTTTTTTTTTTTA+AGG + chr2.2:4766761-4766780 None:intergenic 5.0%
!!! TATCTTTAAAATTATTTATT+GGG + chr2.2:4765956-4765975 None:intergenic 5.0%
!!! TTTTTTTTTAAATTTAAATC+GGG + chr2.2:4767526-4767545 None:intergenic 5.0%
AAAGCCTGCACAACTCTTGC+TGG + chr2.2:4767149-4767168 None:intergenic 50.0%
ACCGGACCCACCAAGAAAAA+CGG + chr2.2:4765672-4765691 None:intergenic 50.0%
ACCTGTCCCACTTCTTGAAC+CGG + chr2.2:4765690-4765709 None:intergenic 50.0%
ATGGGTCAGTTATTCCGGGA+CGG + chr2.2:4768676-4768695 None:intergenic 50.0%
CACCGGTGAGTTGTAACCAA+GGG - chr2.2:4765273-4765292 MS.gene052071:intron 50.0%
CCAGAGTCACAATTCCGAAC+TGG - chr2.2:4765096-4765115 MS.gene052071:intron 50.0%
CCAGTTCGGAATTGTGACTC+TGG + chr2.2:4765099-4765118 None:intergenic 50.0%
CCCTTTAACCACGTTAGCGT+AGG + chr2.2:4765360-4765379 None:intergenic 50.0%
CTCCCTTGGTTACAACTCAC+CGG + chr2.2:4765278-4765297 None:intergenic 50.0%
GGATTCAACTCTGTGGTCAC+CGG - chr2.2:4765256-4765275 MS.gene052071:intron 50.0%
GGTCAATTATACCGGAACGG+AGG - chr2.2:4768871-4768890 MS.gene052071:intron 50.0%
GGTGACCACAGAGTTGAATC+CGG + chr2.2:4765257-4765276 None:intergenic 50.0%
GTAACCAAGGGAGCTCAGAA+GGG - chr2.2:4765285-4765304 MS.gene052071:intron 50.0%
GTCCTCGTCCTCATCTAACA+AGG - chr2.2:4765606-4765625 MS.gene052071:intron 50.0%
GTCTTCCAACAAGGTGTAGC+TGG + chr2.2:4765332-4765351 None:intergenic 50.0%
GTGATGAAATCCTCGTCCGT+TGG + chr2.2:4764998-4765017 None:intergenic 50.0%
GTTCACCGGATTCAACTCTG+TGG - chr2.2:4765249-4765268 MS.gene052071:intron 50.0%
TCACCGGTGAGTTGTAACCA+AGG - chr2.2:4765272-4765291 MS.gene052071:intron 50.0%
TGTAACCAAGGGAGCTCAGA+AGG - chr2.2:4765284-4765303 MS.gene052071:intron 50.0%
TTCCAACAAGGTGTAGCTGG+CGG + chr2.2:4765329-4765348 None:intergenic 50.0%
TTCGGAATTGTGACTCTGGG+AGG + chr2.2:4765095-4765114 None:intergenic 50.0%
!! CGGAGGGAGTACTGAGTATT+CGG + chr2.2:4768656-4768675 None:intergenic 50.0%
!!! ATGCAGGCCGTTTTTCTTGG+TGG - chr2.2:4765662-4765681 MS.gene052071:intron 50.0%
!!! TGCAGGCCGTTTTTCTTGGT+GGG - chr2.2:4765663-4765682 MS.gene052071:intron 50.0%
AGAATGGTACCACGGAGTGG+AGG + chr2.2:4767215-4767234 None:intergenic 55.0%
ATCCTCGTCCGTTGGAACCA+TGG + chr2.2:4764990-4765009 None:intergenic 55.0%
ATGACCCTTCTGAGCTCCCT+TGG + chr2.2:4765292-4765311 None:intergenic 55.0%
CTGCATCATTCCTCCACCAC+TGG + chr2.2:4765648-4765667 None:intergenic 55.0%
GAATGGTACCACGGAGTGGA+GGG + chr2.2:4767214-4767233 None:intergenic 55.0%
GCATGTGATCGGATGGTGAG+TGG - chr2.2:4766808-4766827 MS.gene052071:intron 55.0%
GGAGGGACGCCAACAACTTT+GGG + chr2.2:4767197-4767216 None:intergenic 55.0%
GTCAGTTATTCCGGGACGGA+GGG + chr2.2:4768672-4768691 None:intergenic 55.0%
GTGGGTCCGGTTCAAGAAGT+GGG - chr2.2:4765681-4765700 MS.gene052071:intron 55.0%
TCCGGTTCAAGAAGTGGGAC+AGG - chr2.2:4765686-4765705 MS.gene052071:intron 55.0%
TGGAGGGACGCCAACAACTT+TGG + chr2.2:4767198-4767217 None:intergenic 55.0%
! CAGTGGTGGAGGAATGATGC+AGG - chr2.2:4765646-4765665 MS.gene052071:intron 55.0%
! GGTGTTTTCTTGCCTCGTGC+TGG - chr2.2:4765707-4765726 MS.gene052071:intron 55.0%
!! GGAGCAGGCCTTGTTAGATG+AGG + chr2.2:4765617-4765636 None:intergenic 55.0%
!! GGCCTTGTTAGATGAGGACG+AGG + chr2.2:4765611-4765630 None:intergenic 55.0%
!!! GCCGTTTTTCTTGGTGGGTC+CGG - chr2.2:4765668-4765687 MS.gene052071:intron 55.0%
ACTCAGTACTCCCTCCGTCC+CGG - chr2.2:4768659-4768678 MS.gene052071:CDS 60.0%
CACCATGGTTCCAACGGACG+AGG - chr2.2:4764985-4765004 MS.gene052071:CDS 60.0%
CCATTGCAGACCAGTGGTGG+AGG - chr2.2:4765635-4765654 MS.gene052071:intron 60.0%
CCTGCTCCATTGCAGACCAG+TGG - chr2.2:4765629-4765648 MS.gene052071:intron 60.0%
CTCCGCCAGCTACACCTTGT+TGG - chr2.2:4765324-4765343 MS.gene052071:intron 60.0%
GCAACAACGGTTCCAGCACG+AGG + chr2.2:4765722-4765741 None:intergenic 60.0%
GCTCCATTGCAGACCAGTGG+TGG - chr2.2:4765632-4765651 MS.gene052071:intron 60.0%
GGTCAGTTATTCCGGGACGG+AGG + chr2.2:4768673-4768692 None:intergenic 60.0%
GGTGGGTCCGGTTCAAGAAG+TGG - chr2.2:4765680-4765699 MS.gene052071:intron 60.0%
! CCACTGGTCTGCAATGGAGC+AGG + chr2.2:4765632-4765651 None:intergenic 60.0%
! CCTCCACCACTGGTCTGCAA+TGG + chr2.2:4765638-4765657 None:intergenic 60.0%
GTTGGCGTCCCTCCACTCCG+TGG - chr2.2:4767203-4767222 MS.gene052071:intron 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 4764929 4769166 4764929 ID=MS.gene052071
chr2.2 mRNA 4764929 4769166 4764929 ID=MS.gene052071.t1;Parent=MS.gene052071
chr2.2 exon 4768978 4769166 4768978 ID=MS.gene052071.t1.exon1;Parent=MS.gene052071.t1
chr2.2 CDS 4768978 4769166 4768978 ID=cds.MS.gene052071.t1;Parent=MS.gene052071.t1
chr2.2 exon 4768646 4768862 4768646 ID=MS.gene052071.t1.exon2;Parent=MS.gene052071.t1
chr2.2 CDS 4768646 4768862 4768646 ID=cds.MS.gene052071.t1;Parent=MS.gene052071.t1
chr2.2 exon 4768328 4768603 4768328 ID=MS.gene052071.t1.exon3;Parent=MS.gene052071.t1
chr2.2 CDS 4768328 4768603 4768328 ID=cds.MS.gene052071.t1;Parent=MS.gene052071.t1
chr2.2 exon 4766871 4766975 4766871 ID=MS.gene052071.t1.exon4;Parent=MS.gene052071.t1
chr2.2 CDS 4766871 4766975 4766871 ID=cds.MS.gene052071.t1;Parent=MS.gene052071.t1
chr2.2 exon 4764929 4765041 4764929 ID=MS.gene052071.t1.exon5;Parent=MS.gene052071.t1
chr2.2 CDS 4764929 4765041 4764929 ID=cds.MS.gene052071.t1;Parent=MS.gene052071.t1
Gene Sequence

>MS.gene052071

ATGGCTGATGATAATAATATGTACCATGGTGTTCTCTCACTTAGCAACAACAATCACACCATGGTTCCAACGGACGAGGATTTCATCACCGAGTTAACTCATCAAATGTCTCAATTCATGCTTCAAGAAGATATTGACGACTCATTCGATTTCTCATCTATTGCCTCCCAGAGTCACAATTCCGAACTGGTACCATATGTTTCCCTTACTATCTACGCGCTTCTTTTTCAGGTTTATTGAATAACTGATGTATCTGACCTATATTATTAACTCTTCTTTGTTTTCTTTCTTCCTTTTCCTGTAGTCGTGGGATTTAATTAGTTCACCGGATTCAACTCTGTGGTCACCGGTGAGTTGTAACCAAGGGAGCTCAGAAGGGTCATCACAGAACTCATCTCCGCCAGCTACACCTTGTTGGAAGACAAAAACCTACGCTAACGTGGTTAAAGGGGTTGAGAAAAATACTAAGAAGCTTAAAGAAACAGCGACTTCTAAACAGAATCATGGTTCTGATATTCAAAGTTTCAAGTCCTCCAATATTGATAGATTTTCATCACACAAAGCATTGATTGAGGAACAAATTAAAGCCATTGAATTAAGGAAAAAACATTACATCAAGCAGCAAAATGAGGCTAAAGAAGAGATTCAGAAGCAAGGTAATGGAGGGAACTGTACTCGTCCTCGTCCTCATCTAACAAGGCCTGCTCCATTGCAGACCAGTGGTGGAGGAATGATGCAGGCCGTTTTTCTTGGTGGGTCCGGTTCAAGAAGTGGGACAGGTGTTTTCTTGCCTCGTGCTGGAACCGTTGTTGCTCCATCAGAATCAACCAAAAAGAAAGGTAACGTGGATTATGTTATATCTATTCTGTTCATGTTTTTCACTAGTCGGTAATGGTTGGTTGCGGAATCAAATGGAATGTCTAGTTTCCCACTTTTAATATTCGAATCTGTTTATGATATTTTGTCTCTAAAATTCCACTCTTTTTCTACTTTCTAAGATTTCTTCTTGTTTGTTTCTTTTCAACCCAATAAATAATTTTAAAGATATTTACATGGTTTTTAGTTAAGATTTATGAGCAATCAATCATTTCATTTTAAATGTGTGTGATGTGCTGTTATTATAATCTTCAAGTGTCTTCTTTATTAGTTATTGTGATCACTAAATATGCTTCTTGAGTAATTTAACTTAATCCTTAAATGTATTTTTTTTTTTATCAAGTTAGTTCAAAAGAAACACTAGAAATATGTATGCAATTTTAATAGATTTAAAATGGATCTCCCATTTGTAACTCTACAAGCTTAGTCCTCCAATTTTAAAAGAGAAATGATATTTTGACAACCACTTTTTGACAACTTTTGGACAACTTTCTCTCACATACTCACATGATGTTGTTATCATCTCTCTTCCTTTTTCTCTCTCCATTGTTTTTGACCAATAAGAAGTGAGAAAAACAAGGTTGTCAACAAAGTTGTCATCAAATGGATGTCAAAATATCATTACTCATTTTAAAAACATCAAATATTTTATCCACCTATATGCTATTGAGGTGAAATCCTAAAGTGGCAACTAGATTGATGAGATGAAAATGAGTTATCTAACTAGATTGATGCTATTAAGGGTGCATTAAATGTAGTAACTCTTACGATATAAGCTCATGAGGACATAGAAAATGAAAGCTAATTCAAGTGATAATCCATCTAAATATGTCATGTCATCCACTAAGTTGTCACATAAACATGTAGATTTTTTTTTAAAAAAAATATTCTTAATATTACGCTTCATTAATCTCCACTAATCATCCACATTTAGCCTATCTAGCTAGCTCCTTCCTTAAAAAAAAAAAAAAAAAACCTATCTATCTTGCCTTTGTACTAGTTTGCATGTGATCGGATGGTGAGTGGTGACCAAATATTCTTTATGGTGGTTGAACTTGATTTTTCCTTAATCTATAGTAACATGCTGCTGACATTTGAGTTGATGATTTGTTCCTTTCGGTTCTGTTTTATTTTTCATTTAGCAAGAAACATCAAAAAATTAAGCAGTTAGAAAGTTTGGTGAGCAAAATAGATTCGTTAATGTTTGAAGGAATCCTTAATTAATTATACTTAACTGCCAAAACAATACTATCAAATTCGTTTCACATCTTACATCAAAATTGATTTGTTTTATGCACGTTATATATATTGTTAGGGAAAGGTTGCTCCACTGTCCTTATACCAGCAAGAGTTGTGCAGGCTTTGCAACTACACTTTGACAAAACGGCTCCCAAAGTTGTTGGCGTCCCTCCACTCCGTGGTACCATTCTTAATTTATTATTGTTAATTATACCATCAAGCAACAACTTTGATTTGTTGTTTATTTATTGCTATTCTTCATGCCGTTTAGTTACTACTCTCCTAATAAATAAATAATCTTACCCAACAAAATATTTGGATGAGAAGATAAGAATCTCTTCCAAGTTAACTAAAAGTCTTAGGTTCGAATCAAAACTCTAAAAATACAACAATATTAAATCTTTTTAAGGAGAATTTTTTTACCATTGTAATCCTAATCTATTCGAGATGTTAGTTTGTGCAATTATTCGTATAGGACACCCGATTTAAATTTAAAAAAAAAAAAACTCATGACCATGAATTTGTTGCTTCATCCTTAGCAACATGACATTCAATAAACTTTTAGTTGTTATATCAATTCCCATTTTATATGCATATAAAATTTATTTGGGTAATTCCTAAGTTTAACGCTCTAAGTTTGGTTGTATTCAATATAGTCTATCAATTTAAATGATTATCCTTTCTTTTTAAAATTATTTGGGCAATTCCTAAGTTTTATACTCTTAAGTTTTGTAGCACCAAAAATAAAATACTCTTAAGTTTTGTAGAAAGAAAAAACTCTAAGTTTGCTACTGACTTTCTTATTTCATTTTCTTATCAATCATATAAATTTATAAACAATTTCTACATGAAAAATTCTATGGTGAAGTCATGAGAATGACTATTTTGGTGAAGTTAACACTATAACATCAAAAATGTAATGAGTAACACCTCACTGTTTGTACAATGACATCAAAAGTTCAGTCCTCATAGTATCATCAATTCATCAGATTAACAAGATTACACTTAATCTAATTTTATCCTACCTTGTTATAAGCGTAACACCCCACAAAGTGTAACACCTAAGACTATCACATAATATCATCAACTCTGCTTGCATGTTAAAGATTCCGACAATAAAATAGTACCTGTAAATCCAAGATGTTGCAAGCATTACTAGATGAATTATTATTGATCATTAATCAGTAATCAACAATTTAAAAATGAATCAAACATTAAGAACAAAATTAACTTTTTCAAAAAGTCAGTTGTCTGACTGCACTATAGAAGCTCCCTTTCTACATTTGTTTAATTTATTCACTCTTTGATTTCCTATCAATTTCTCATCTGTTTGCTTACACTCAAGTCTTGCGCAATGTTAGATTGTATTAAATGTCACACTCACACACACATCATCTAGTTGTTTTTTTATACTTTCTTTAGAAAAATTGGCATACGTCATGTTTAAAAAAAACAAAAATTATGAATAAATTAAAACTAGTATATTCTATGAAAGACTTCTAGTTAGTACTAATCTCTCTATGATTACTTTGATTTGCAATTCTTAATAATGGAATGTATTAGCTGTCCAATTTTATTTTTCGAGACTGCTGCAATTTATTCCAACAGTCCGAATACTCAGTACTCCCTCCGTCCCGGAATAACTGACCCATTTTCTAAAAAAAAGTGTCTCAAAATAACTGACCAATTTCAATTTTCAATACAACTTTCATCATTCCCAATACAATATAATACTAAATAATACTACTTTTATCATTCCCAATACAATATATTCCACTTTCCCTTTTATTTATACACTTTTCTTAATCTGTGTGAAAAACCAAAGTAGGTCAATTATACCGGAACGGAGGTAGTATCAACTAGCAATTTGTCGCCAACTAGTTCTAAAATATTGAACTCGTAGTTATTGATTATATTCATAACACTGTCTAATGTGATTTTCATCTATTTATTTATTTATTTGTTGTTAATCAATTTGCTCTATAATATTGTTATTGTTATGAATTGCAGATCTTGTTTTGAACCATAGGGAAGGTATGTTTTCACTTGAGAATCACCAGTCAACAAAAGCAGCAACAGATGTCCAGAATGATATGATTCTGCCTCAAGAATGGACATATTGA
Protein sequence

>MS.gene052071.t1

MADDNNMYHGVLSLSNNNHTMVPTDEDFITELTHQMSQFMLQEDIDDSFDFSSIASQSHNSELSWDLISSPDSTLWSPVSCNQGSSEGSSQNSSPPATPCWKTKTYANVVKGVEKNTKKLKETATSKQNHGSDIQTLIEEQIKAIELRKKHYIKQQNEAKEEIQKQGNGGNCTRPRPHLTRPAPLQTSGGGMMQAVFLGGSGSRSGTGVFLPRAGTVVAPSESTKKKGKGCSTVLIPARVVQALQLHFDKTAPKVVGVPPLRDLVLNHREGMFSLENHQSTKAATDVQNDMILPQEWTY