AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31121

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31121.t1 MTR_2g097620 97.125 313 6 1 1 313 1 310 0.0 624
MS.gene31121.t1 MTR_4g064570 66.144 319 89 7 1 313 1 306 2.85e-137 394
MS.gene31121.t1 MTR_3g068005 51.652 333 122 5 9 306 2 330 6.95e-110 326
MS.gene31121.t1 MTR_5g080350 44.688 320 143 8 12 307 2 311 3.17e-82 255
MS.gene31121.t1 MTR_1g114320 32.184 261 146 8 14 258 13 258 9.43e-40 142
MS.gene31121.t1 MTR_5g088720 34.586 266 121 9 6 237 4 250 2.51e-38 139
MS.gene31121.t1 MTR_5g088720 34.601 263 119 9 9 237 2 245 3.88e-38 139
MS.gene31121.t1 MTR_5g088720 34.944 269 122 10 3 237 42 291 4.82e-38 139
MS.gene31121.t1 MTR_0067s0100 35.656 244 131 8 9 237 3 235 1.58e-35 130
MS.gene31121.t1 MTR_1g051105 32.035 231 125 6 9 237 4 204 9.22e-33 122
MS.gene31121.t1 MTR_6g079660 46.903 113 56 2 135 246 180 289 4.41e-31 120
MS.gene31121.t1 MTR_3g072630 28.968 252 146 4 14 237 20 266 2.11e-30 118
MS.gene31121.t1 MTR_3g072630 28.968 252 146 4 14 237 20 266 3.51e-30 117
MS.gene31121.t1 MTR_3g083480 40.940 149 73 5 135 278 188 326 5.32e-28 112
MS.gene31121.t1 MTR_1g114620 50.000 102 45 3 145 245 221 317 5.83e-26 106
MS.gene31121.t1 MTR_6g032960 41.026 117 63 3 124 240 174 284 1.66e-24 102
MS.gene31121.t1 MTR_2g007340 43.519 108 59 1 149 254 158 265 2.11e-23 99.0
MS.gene31121.t1 MTR_7g074170 40.000 115 65 2 131 244 286 397 1.61e-22 98.6
MS.gene31121.t1 MTR_4g110100 38.739 111 64 3 137 246 307 414 2.56e-22 97.4
MS.gene31121.t1 MTR_3g088080 43.902 82 44 1 157 238 5 84 1.28e-21 87.8
MS.gene31121.t1 MTR_6g016350 46.809 94 49 1 152 244 270 363 1.54e-19 89.0
MS.gene31121.t1 MTR_1g014710 36.893 103 62 1 146 245 184 286 4.80e-19 86.7
MS.gene31121.t1 MTR_5g041310 35.714 112 64 2 129 240 60 163 1.33e-17 80.9
MS.gene31121.t1 MTR_5g041330 40.000 80 45 1 162 241 78 154 1.59e-16 77.8
MS.gene31121.t1 MTR_2g061790 61.905 42 16 0 192 233 109 150 2.67e-14 71.6
MS.gene31121.t1 MTR_3g031000 41.667 72 34 2 167 238 279 342 1.82e-12 67.8
MS.gene31121.t1 MTR_5g022920 29.375 160 93 5 97 236 75 234 6.22e-12 65.1
MS.gene31121.t1 MTR_3g025510 35.938 64 41 0 170 233 83 146 8.19e-12 63.2
MS.gene31121.t1 MTR_4g080750 44.444 54 30 0 180 233 173 226 4.62e-11 63.2
MS.gene31121.t1 MTR_5g058530 36.364 88 40 3 179 258 210 289 4.95e-11 63.5
MS.gene31121.t1 MTR_1g095030 58.333 36 15 0 198 233 18 53 8.97e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31121.t1 AT5G59550 51.961 306 122 6 13 304 17 311 5.16e-93 283
MS.gene31121.t1 AT5G59550 51.961 306 122 6 13 304 122 416 4.25e-92 284
MS.gene31121.t1 AT3G46620 52.459 305 122 8 13 304 30 324 4.43e-92 280
MS.gene31121.t1 AT2G39720 44.985 329 116 10 10 293 2 310 2.57e-83 258
MS.gene31121.t1 AT2G39720 44.985 329 116 10 10 293 2 310 2.57e-83 258
MS.gene31121.t1 AT1G55530 27.817 284 141 7 12 245 7 276 3.38e-36 134
MS.gene31121.t1 AT1G55530 27.817 284 141 7 12 245 7 276 3.38e-36 134
MS.gene31121.t1 AT3G56580 34.694 245 122 7 12 237 6 231 3.18e-35 130
MS.gene31121.t1 AT3G56580 34.694 245 122 7 12 237 6 231 3.18e-35 130
MS.gene31121.t1 AT3G56580 34.694 245 122 7 12 237 6 231 3.18e-35 130
MS.gene31121.t1 AT3G19950 30.070 286 133 8 3 246 10 270 3.36e-35 131
MS.gene31121.t1 AT3G19950 30.070 286 133 8 3 246 68 328 6.78e-35 131
MS.gene31121.t1 AT3G19950 30.070 286 133 8 3 246 68 328 6.78e-35 131
MS.gene31121.t1 AT2G40830 34.387 253 140 7 9 246 3 244 6.95e-35 130
MS.gene31121.t1 AT2G40830 34.387 253 140 7 9 246 3 244 6.95e-35 130
MS.gene31121.t1 AT2G40830 34.387 253 140 7 9 246 3 244 6.95e-35 130
MS.gene31121.t1 AT2G40830 34.387 253 140 7 9 246 3 244 6.95e-35 130
MS.gene31121.t1 AT1G55530 30.303 264 129 7 11 245 6 243 1.88e-34 129
MS.gene31121.t1 AT3G13430 30.182 275 140 9 9 238 4 271 8.08e-32 121
MS.gene31121.t1 AT3G13430 30.182 275 140 9 9 238 4 271 8.08e-32 121
MS.gene31121.t1 AT3G13430 30.182 275 140 9 9 238 4 271 8.08e-32 121
MS.gene31121.t1 AT3G13430 30.182 275 140 9 9 238 4 271 8.08e-32 121
MS.gene31121.t1 AT3G10815 58.974 78 32 0 166 243 95 172 5.44e-28 108
MS.gene31121.t1 AT5G01980 42.735 117 64 1 128 244 289 402 1.06e-25 107
MS.gene31121.t1 AT5G08139 43.689 103 54 2 145 246 264 363 1.57e-25 105
MS.gene31121.t1 AT5G56340 44.231 104 50 2 135 238 210 305 3.41e-24 102
MS.gene31121.t1 AT1G60360 42.453 106 55 2 142 246 178 278 5.04e-24 100
MS.gene31121.t1 AT5G15820 44.681 94 51 1 154 246 252 345 1.02e-23 100
MS.gene31121.t1 AT3G02340 35.032 157 89 3 120 265 252 406 3.38e-23 99.8
MS.gene31121.t1 AT3G02340 35.032 157 89 3 120 265 252 406 3.38e-23 99.8
MS.gene31121.t1 AT4G26400 40.952 105 54 2 135 239 192 288 2.12e-22 96.7
MS.gene31121.t1 AT4G26400 40.952 105 54 2 135 239 192 288 2.12e-22 96.7
MS.gene31121.t1 AT5G64920 44.231 104 57 1 142 244 206 309 3.24e-22 95.9
MS.gene31121.t1 AT1G68180 53.521 71 33 0 166 236 112 182 5.08e-22 93.6
MS.gene31121.t1 AT3G60080 55.405 74 25 1 173 238 142 215 2.61e-21 92.8
MS.gene31121.t1 AT5G60820 44.211 95 45 3 150 239 328 419 7.67e-20 90.1
MS.gene31121.t1 AT5G20910 35.398 113 64 3 136 245 177 283 1.04e-19 88.6
MS.gene31121.t1 AT2G44330 42.157 102 45 2 173 260 71 172 2.66e-19 84.7
MS.gene31121.t1 AT1G26800 40.541 111 57 3 129 239 59 160 1.64e-17 80.1
MS.gene31121.t1 AT1G14200 29.448 163 85 3 75 237 22 154 2.69e-15 73.6
MS.gene31121.t1 AT5G02750 50.000 56 28 0 189 244 211 266 1.07e-14 73.9
MS.gene31121.t1 AT3G30460 43.662 71 36 2 172 238 75 145 3.66e-13 66.6
MS.gene31121.t1 AT3G30460 43.662 71 36 2 172 238 111 181 5.74e-13 67.0

Find 100 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGGCGGGAGGTTTGTCTTC+TGG 0.283501 2.4:-6238854 None:intergenic
GCAAACTGCACAGTGAATTT+CGG 0.334109 2.4:-6239232 None:intergenic
AAGCGGTTGGATTGACGATA+TGG 0.341428 2.4:+6239430 MS.gene31121:CDS
GCGTCTGAGAGTAGAATTTC+CGG 0.352120 2.4:+6239393 MS.gene31121:CDS
ACCGCCGCCGGCGTCTGCAC+CGG 0.356131 2.4:-6238962 None:intergenic
GAGGACAAACGACGTTGTTT+TGG 0.359117 2.4:-6238750 None:intergenic
CATCCTCAACGACGTCGTTT+TGG 0.362996 2.4:+6238692 MS.gene31121:CDS
AGAACGATTACAGGATTAAA+AGG 0.369503 2.4:-6238907 None:intergenic
GCCGGCGTCTGCACCGGCAC+CGG 0.370020 2.4:-6238956 None:intergenic
TCTCTTCGACGAATCCGTTT+TGG 0.397147 2.4:-6238777 None:intergenic
CCGGCGGTGGTGTCTTCTCT+AGG 0.412632 2.4:-6238937 None:intergenic
GCCGGTGCAGACGCCGGCGG+CGG 0.414591 2.4:+6238961 MS.gene31121:CDS
TCGGAGCTATTACTCGGTTC+CGG 0.420389 2.4:+6239078 MS.gene31121:CDS
TACTCGGTTCCGGCTTCGAT+CGG 0.428438 2.4:+6239088 MS.gene31121:CDS
ATCAACAAACATACATAGTT+GGG 0.428805 2.4:+6239896 MS.gene31121:CDS
GTCTGCACCGGCACCGGCGG+TGG 0.429367 2.4:-6238950 None:intergenic
GATCAACAAACATACATAGT+TGG 0.431507 2.4:+6239895 MS.gene31121:CDS
TTCGGATTCGATTTCTGAAT+CGG 0.431784 2.4:-6239214 None:intergenic
GCAGACGCCGGCGGCGGTAG+CGG 0.432430 2.4:+6238967 MS.gene31121:CDS
GGTCCACGGAGAACGATTAC+AGG 0.435957 2.4:-6238916 None:intergenic
TCTATCGCCGCCTTTGACGC+CGG 0.437391 2.4:-6239168 None:intergenic
CAAATCGAAATCAACGGTTT+CGG 0.449596 2.4:+6239129 MS.gene31121:CDS
CTCTAACAACCGATCGAAGC+CGG 0.463112 2.4:-6239097 None:intergenic
ATTGACGATATGGAGGTTAC+CGG 0.464101 2.4:+6239440 MS.gene31121:CDS
GTTTGTCCTCACTGCCAAAA+CGG 0.475086 2.4:+6238763 MS.gene31121:CDS
TAGAAGAATTTCTTTGACTG+TGG 0.477614 2.4:+6239569 MS.gene31121:CDS
TTCGCCGGTGGTCGGAGAGC+CGG 0.489916 2.4:+6239486 MS.gene31121:CDS
GCCGGTGCCGGTGCAGACGC+CGG 0.490347 2.4:+6238955 MS.gene31121:CDS
TCCGACATGCTTGGTGGAAG+TGG 0.494594 2.4:-6239060 None:intergenic
AGAAACCGCCGGTGATTGAC+CGG 0.496031 2.4:-6238806 None:intergenic
CGCTTCTTCATCAATCTGAC+CGG 0.496970 2.4:-6239412 None:intergenic
ATCAACACCGCTACCGCCGC+CGG 0.500661 2.4:-6238974 None:intergenic
GGCGTTACGACGTCTGCGGC+GGG 0.502562 2.4:-6238869 None:intergenic
AAAAGGCGAACGGCTGCCGC+CGG 0.509087 2.4:-6238890 None:intergenic
TCCATCTCCGTGTAAACAAT+CGG 0.509848 2.4:-6239522 None:intergenic
TTCAATGCGAAATTCGTGTC+CGG 0.510386 2.4:+6239338 MS.gene31121:CDS
ACCGATTGTTTACACGGAGA+TGG 0.520025 2.4:+6239521 MS.gene31121:CDS
GGTGCCGGTGCAGACGCCGG+CGG 0.520180 2.4:+6238958 MS.gene31121:CDS
GGAGAATCCACCGGCGTCAA+AGG 0.524083 2.4:+6239158 MS.gene31121:CDS
CAGATTGATGAAGAAGCGGT+TGG 0.524412 2.4:+6239417 MS.gene31121:CDS
TTATTGCAGGGTGCAAGATA+AGG 0.524819 2.4:+6239857 MS.gene31121:intron
CCGGGAGGTGGATTTGCTGT+TGG 0.525108 2.4:+6239459 MS.gene31121:CDS
TTGGAAGATTCGCCGGTGGT+CGG 0.527536 2.4:+6239478 MS.gene31121:CDS
GAAGACACCACCGCCGGTGC+CGG 0.529621 2.4:+6238943 MS.gene31121:CDS
ATATGGAGGTTACCGGGAGG+TGG 0.532142 2.4:+6239447 MS.gene31121:CDS
ATTCCGATTGCATTCTCCCA+TGG 0.535454 2.4:+6239313 MS.gene31121:CDS
ATCGCCGCCTTTGACGCCGG+TGG 0.538439 2.4:-6239165 None:intergenic
TGCAAAGAACAATTCGAGCT+AGG 0.538605 2.4:+6239252 MS.gene31121:CDS
AAGCCATGGGAGAATGCAAT+CGG 0.539317 2.4:-6239316 None:intergenic
GGCGTCTGCACCGGCACCGG+CGG 0.539489 2.4:-6238953 None:intergenic
AATAGCTCCGACATGCTTGG+TGG 0.540581 2.4:-6239066 None:intergenic
TGGTGGAAGTGGACGTAGAT+CGG 0.541171 2.4:-6239049 None:intergenic
AGAAGAATTTCTTTGACTGT+GGG 0.543294 2.4:+6239570 MS.gene31121:CDS
GATTCACGCCGGTCAATCAC+CGG 0.546800 2.4:+6238798 MS.gene31121:CDS
GCGGGAGGTTTGTCTTCTGG+AGG 0.550711 2.4:-6238851 None:intergenic
GAATCGGTGATTTCAACTGT+TGG 0.552700 2.4:-6239198 None:intergenic
TCAGTTACCGATTGTTTACA+CGG 0.553870 2.4:+6239515 MS.gene31121:CDS
CGCCGCAGACGTCGTAACGC+CGG 0.555138 2.4:+6238871 MS.gene31121:CDS
AGTAATAGCTCCGACATGCT+TGG 0.555247 2.4:-6239069 None:intergenic
GAATTTCGCATTGAAAGCCA+TGG 0.556326 2.4:-6239330 None:intergenic
CGGTCAGATTGATGAAGAAG+CGG 0.556476 2.4:+6239413 MS.gene31121:CDS
CGCAGACGTCGTAACGCCGG+CGG 0.559927 2.4:+6238874 MS.gene31121:CDS
GAAATTCGTGTCCGGTGTGT+CGG 0.561109 2.4:+6239346 MS.gene31121:CDS
CGGCGTTACGACGTCTGCGG+CGG 0.562313 2.4:-6238870 None:intergenic
TTCTCTCAAATCGAAATCAA+CGG 0.566290 2.4:+6239123 MS.gene31121:CDS
GCACCAAAACGACGTCGTTG+AGG 0.569778 2.4:-6238695 None:intergenic
AATCAACGGTTTCGGAAGGT+CGG 0.570591 2.4:+6239137 MS.gene31121:CDS
CGGAAGGTCGGAGAATCCAC+CGG 0.582211 2.4:+6239149 MS.gene31121:CDS
CAAAACGACGTCGTTGAGGA+TGG 0.582320 2.4:-6238691 None:intergenic
TGGTGTCTTCTCTAGGTCCA+CGG 0.584451 2.4:-6238930 None:intergenic
CGGTAGCGGTGTTGATAGCG+AGG 0.586301 2.4:+6238981 MS.gene31121:CDS
TTTGCTGTTGGAAGATTCGC+CGG 0.595189 2.4:+6239471 MS.gene31121:CDS
ATTGTTTACACGGAGATGGA+TGG 0.596456 2.4:+6239525 MS.gene31121:CDS
TTGACGATATGGAGGTTACC+GGG 0.603171 2.4:+6239441 MS.gene31121:CDS
TCGAAATCAACGGTTTCGGA+AGG 0.605406 2.4:+6239133 MS.gene31121:CDS
CGCCGGCGTTACGACGTCTG+CGG 0.608354 2.4:-6238873 None:intergenic
CCAACAGCAAATCCACCTCC+CGG 0.611076 2.4:-6239459 None:intergenic
AATTTCGCATTGAAAGCCAT+GGG 0.617522 2.4:-6239329 None:intergenic
GTTACGACGTCTGCGGCGGG+AGG 0.618296 2.4:-6238866 None:intergenic
CTCACCGGCTCTCCGACCAC+CGG 0.618605 2.4:-6239490 None:intergenic
GAATCCACCGGCGTCAAAGG+CGG 0.619728 2.4:+6239161 MS.gene31121:CDS
AGCATGTCGGAGCTATTACT+CGG 0.620643 2.4:+6239072 MS.gene31121:CDS
AATCGGTAACTGAATCTCAC+CGG 0.623794 2.4:-6239505 None:intergenic
ATGGGAGAATGCAATCGGAA+TGG 0.625343 2.4:-6239311 None:intergenic
CCTAGAGAAGACACCACCGC+CGG 0.626450 2.4:+6238937 MS.gene31121:CDS
CGGTTGGATTGACGATATGG+AGG 0.630916 2.4:+6239433 MS.gene31121:CDS
GGTAGCGGTGTTGATAGCGA+GGG 0.633690 2.4:+6238982 MS.gene31121:CDS
GGTAACTCGTGCCGACACAC+CGG 0.638241 2.4:-6239357 None:intergenic
TCCACTTCCACCAAGCATGT+CGG 0.655861 2.4:+6239059 MS.gene31121:CDS
AATCCTGTAATCGTTCTCCG+TGG 0.656883 2.4:+6238913 MS.gene31121:CDS
TCACGCCGGTCAATCACCGG+CGG 0.670342 2.4:+6238801 MS.gene31121:CDS
TCGGCGAAAAGAGAAACCGC+CGG 0.670628 2.4:-6238817 None:intergenic
GAATGGTAAAGATGATTACA+CGG 0.678082 2.4:-6239294 None:intergenic
GTAGCGGTGTTGATAGCGAG+GGG 0.678382 2.4:+6238983 MS.gene31121:CDS
TTATACTATGAAGACAGTAA+CGG 0.680959 2.4:+6239024 MS.gene31121:CDS
TTACAGGATTAAAAGGCGAA+CGG 0.681942 2.4:-6238900 None:intergenic
GCTGTTGGAAGATTCGCCGG+TGG 0.685393 2.4:+6239474 MS.gene31121:CDS
TCTGGAGGACTGAATACCGT+CGG 0.709231 2.4:-6238836 None:intergenic
TTCGTCGAAGAGATTCACGC+CGG 0.729026 2.4:+6238787 MS.gene31121:CDS
ACGATATGGAGGTTACCGGG+AGG 0.773215 2.4:+6239444 MS.gene31121:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAATTTTTTATCGTTTTTT+GGG + chr2.4:6239637-6239656 MS.gene31121:intron 15.0%
!! TACAGAAGAATAATATGATT+TGG + chr2.4:6239734-6239753 MS.gene31121:intron 20.0%
!!! CGAAATTTTTTATCGTTTTT+TGG + chr2.4:6239636-6239655 MS.gene31121:intron 20.0%
!!! TAATATGATTTGGTTTTGTT+TGG + chr2.4:6239744-6239763 MS.gene31121:intron 20.0%
! ATCAACAAACATACATAGTT+GGG + chr2.4:6239896-6239915 MS.gene31121:CDS 25.0%
! TTATACTATGAAGACAGTAA+CGG + chr2.4:6239024-6239043 MS.gene31121:CDS 25.0%
! TTTAGTAGATGATTATTGCA+GGG + chr2.4:6239845-6239864 MS.gene31121:intron 25.0%
AGAACGATTACAGGATTAAA+AGG - chr2.4:6238910-6238929 None:intergenic 30.0%
AGAAGAATTTCTTTGACTGT+GGG + chr2.4:6239570-6239589 MS.gene31121:CDS 30.0%
GAATGGTAAAGATGATTACA+CGG - chr2.4:6239297-6239316 None:intergenic 30.0%
GATCAACAAACATACATAGT+TGG + chr2.4:6239895-6239914 MS.gene31121:CDS 30.0%
GTTTAGTAGATGATTATTGC+AGG + chr2.4:6239844-6239863 MS.gene31121:intron 30.0%
TAGAAGAATTTCTTTGACTG+TGG + chr2.4:6239569-6239588 MS.gene31121:CDS 30.0%
TTCTCTCAAATCGAAATCAA+CGG + chr2.4:6239123-6239142 MS.gene31121:CDS 30.0%
! AACTTAAATCGTAGCTTTTC+TGG + chr2.4:6239666-6239685 MS.gene31121:intron 30.0%
AAGAAGAAGAAGCAGAACTT+GGG + chr2.4:6239710-6239729 MS.gene31121:intron 35.0%
AATTTCGCATTGAAAGCCAT+GGG - chr2.4:6239332-6239351 None:intergenic 35.0%
CAAATCGAAATCAACGGTTT+CGG + chr2.4:6239129-6239148 MS.gene31121:CDS 35.0%
TTACAGGATTAAAAGGCGAA+CGG - chr2.4:6238903-6238922 None:intergenic 35.0%
TTCGGATTCGATTTCTGAAT+CGG - chr2.4:6239217-6239236 None:intergenic 35.0%
! TCAGTTACCGATTGTTTACA+CGG + chr2.4:6239515-6239534 MS.gene31121:CDS 35.0%
AATAGAGTTAGAGAGAGACG+TGG + chr2.4:6239597-6239616 MS.gene31121:intron 40.0%
AATCGGTAACTGAATCTCAC+CGG - chr2.4:6239508-6239527 None:intergenic 40.0%
ATTGACGATATGGAGGTTAC+CGG + chr2.4:6239440-6239459 MS.gene31121:CDS 40.0%
ATTGTTTACACGGAGATGGA+TGG + chr2.4:6239525-6239544 MS.gene31121:CDS 40.0%
CAAGAAGAAGAAGCAGAACT+TGG + chr2.4:6239709-6239728 MS.gene31121:intron 40.0%
GAATCGGTGATTTCAACTGT+TGG - chr2.4:6239201-6239220 None:intergenic 40.0%
GAATTTCGCATTGAAAGCCA+TGG - chr2.4:6239333-6239352 None:intergenic 40.0%
GCAAACTGCACAGTGAATTT+CGG - chr2.4:6239235-6239254 None:intergenic 40.0%
TCCATCTCCGTGTAAACAAT+CGG - chr2.4:6239525-6239544 None:intergenic 40.0%
TGCAAAGAACAATTCGAGCT+AGG + chr2.4:6239252-6239271 MS.gene31121:CDS 40.0%
TTAGAGAGAGACGTGGAATT+CGG + chr2.4:6239604-6239623 MS.gene31121:intron 40.0%
TTATTGCAGGGTGCAAGATA+AGG + chr2.4:6239857-6239876 MS.gene31121:intron 40.0%
TTCAATGCGAAATTCGTGTC+CGG + chr2.4:6239338-6239357 MS.gene31121:CDS 40.0%
AAGCCATGGGAGAATGCAAT+CGG - chr2.4:6239319-6239338 None:intergenic 45.0%
AAGCGGTTGGATTGACGATA+TGG + chr2.4:6239430-6239449 MS.gene31121:CDS 45.0%
AATCAACGGTTTCGGAAGGT+CGG + chr2.4:6239137-6239156 MS.gene31121:CDS 45.0%
AATCCTGTAATCGTTCTCCG+TGG + chr2.4:6238913-6238932 MS.gene31121:CDS 45.0%
ACCGATTGTTTACACGGAGA+TGG + chr2.4:6239521-6239540 MS.gene31121:CDS 45.0%
AGCATGTCGGAGCTATTACT+CGG + chr2.4:6239072-6239091 MS.gene31121:CDS 45.0%
AGTAATAGCTCCGACATGCT+TGG - chr2.4:6239072-6239091 None:intergenic 45.0%
ATGGGAGAATGCAATCGGAA+TGG - chr2.4:6239314-6239333 None:intergenic 45.0%
ATTCCGATTGCATTCTCCCA+TGG + chr2.4:6239313-6239332 MS.gene31121:CDS 45.0%
CAGATTGATGAAGAAGCGGT+TGG + chr2.4:6239417-6239436 MS.gene31121:CDS 45.0%
CGCTTCTTCATCAATCTGAC+CGG - chr2.4:6239415-6239434 None:intergenic 45.0%
CGGTCAGATTGATGAAGAAG+CGG + chr2.4:6239413-6239432 MS.gene31121:CDS 45.0%
GCGTCTGAGAGTAGAATTTC+CGG + chr2.4:6239393-6239412 MS.gene31121:CDS 45.0%
GTTTGTCCTCACTGCCAAAA+CGG + chr2.4:6238763-6238782 MS.gene31121:CDS 45.0%
TCGAAATCAACGGTTTCGGA+AGG + chr2.4:6239133-6239152 MS.gene31121:CDS 45.0%
TTGACGATATGGAGGTTACC+GGG + chr2.4:6239441-6239460 MS.gene31121:CDS 45.0%
! GAGGACAAACGACGTTGTTT+TGG - chr2.4:6238753-6238772 None:intergenic 45.0%
! TCTCTTCGACGAATCCGTTT+TGG - chr2.4:6238780-6238799 None:intergenic 45.0%
!! ACTCTCAGACGCGTTTTGAT+CGG - chr2.4:6239385-6239404 None:intergenic 45.0%
!! TTTGCTGTTGGAAGATTCGC+CGG + chr2.4:6239471-6239490 MS.gene31121:CDS 45.0%
AATAGCTCCGACATGCTTGG+TGG - chr2.4:6239069-6239088 None:intergenic 50.0%
CAAAACGACGTCGTTGAGGA+TGG - chr2.4:6238694-6238713 None:intergenic 50.0%
CGGTTGGATTGACGATATGG+AGG + chr2.4:6239433-6239452 MS.gene31121:CDS 50.0%
CTCTAACAACCGATCGAAGC+CGG - chr2.4:6239100-6239119 None:intergenic 50.0%
GAAATTCGTGTCCGGTGTGT+CGG + chr2.4:6239346-6239365 MS.gene31121:CDS 50.0%
TCCACTTCCACCAAGCATGT+CGG + chr2.4:6239059-6239078 MS.gene31121:CDS 50.0%
TCGGAGCTATTACTCGGTTC+CGG + chr2.4:6239078-6239097 MS.gene31121:CDS 50.0%
TCTGGAGGACTGAATACCGT+CGG - chr2.4:6238839-6238858 None:intergenic 50.0%
TGGTGGAAGTGGACGTAGAT+CGG - chr2.4:6239052-6239071 None:intergenic 50.0%
TGGTGTCTTCTCTAGGTCCA+CGG - chr2.4:6238933-6238952 None:intergenic 50.0%
TTCGTCGAAGAGATTCACGC+CGG + chr2.4:6238787-6238806 MS.gene31121:CDS 50.0%
! CATCCTCAACGACGTCGTTT+TGG + chr2.4:6238692-6238711 MS.gene31121:CDS 50.0%
!! ACGAATCCGTTTTGGCAGTG+AGG - chr2.4:6238772-6238791 None:intergenic 50.0%
!! TCAGACGCGTTTTGATCGGA+CGG - chr2.4:6239381-6239400 None:intergenic 50.0%
ACGATATGGAGGTTACCGGG+AGG + chr2.4:6239444-6239463 MS.gene31121:CDS 55.0%
AGAAACCGCCGGTGATTGAC+CGG - chr2.4:6238809-6238828 None:intergenic 55.0%
ATATGGAGGTTACCGGGAGG+TGG + chr2.4:6239447-6239466 MS.gene31121:CDS 55.0%
CCAACAGCAAATCCACCTCC+CGG - chr2.4:6239462-6239481 None:intergenic 55.0%
GATTCACGCCGGTCAATCAC+CGG + chr2.4:6238798-6238817 MS.gene31121:CDS 55.0%
GCACCAAAACGACGTCGTTG+AGG - chr2.4:6238698-6238717 None:intergenic 55.0%
GGTCCACGGAGAACGATTAC+AGG - chr2.4:6238919-6238938 None:intergenic 55.0%
TACTCGGTTCCGGCTTCGAT+CGG + chr2.4:6239088-6239107 MS.gene31121:CDS 55.0%
TCGGCGAAAAGAGAAACCGC+CGG - chr2.4:6238820-6238839 None:intergenic 55.0%
TTGGAAGATTCGCCGGTGGT+CGG + chr2.4:6239478-6239497 MS.gene31121:CDS 55.0%
! GCGGTTTCTCTTTTCGCCGA+CGG + chr2.4:6238820-6238839 MS.gene31121:CDS 55.0%
! TCCGACATGCTTGGTGGAAG+TGG - chr2.4:6239063-6239082 None:intergenic 55.0%
!! GGTAGCGGTGTTGATAGCGA+GGG + chr2.4:6238982-6239001 MS.gene31121:CDS 55.0%
!! GTAGCGGTGTTGATAGCGAG+GGG + chr2.4:6238983-6239002 MS.gene31121:CDS 55.0%
CCTAGAGAAGACACCACCGC+CGG + chr2.4:6238937-6238956 MS.gene31121:CDS 60.0%
CGGAAGGTCGGAGAATCCAC+CGG + chr2.4:6239149-6239168 MS.gene31121:CDS 60.0%
GAATCCACCGGCGTCAAAGG+CGG + chr2.4:6239161-6239180 MS.gene31121:CDS 60.0%
GCGGGAGGTTTGTCTTCTGG+AGG - chr2.4:6238854-6238873 None:intergenic 60.0%
GCTGTTGGAAGATTCGCCGG+TGG + chr2.4:6239474-6239493 MS.gene31121:CDS 60.0%
GGAGAATCCACCGGCGTCAA+AGG + chr2.4:6239158-6239177 MS.gene31121:CDS 60.0%
TCTATCGCCGCCTTTGACGC+CGG - chr2.4:6239171-6239190 None:intergenic 60.0%
! GGTAACTCGTGCCGACACAC+CGG - chr2.4:6239360-6239379 None:intergenic 60.0%
!! CCGGGAGGTGGATTTGCTGT+TGG + chr2.4:6239459-6239478 MS.gene31121:CDS 60.0%
!! CGGTAGCGGTGTTGATAGCG+AGG + chr2.4:6238981-6239000 MS.gene31121:CDS 60.0%
AAAAGGCGAACGGCTGCCGC+CGG - chr2.4:6238893-6238912 None:intergenic 65.0%
ATCAACACCGCTACCGCCGC+CGG - chr2.4:6238977-6238996 None:intergenic 65.0%
CCGGCGGTGGTGTCTTCTCT+AGG - chr2.4:6238940-6238959 None:intergenic 65.0%
GCGGCGGGAGGTTTGTCTTC+TGG - chr2.4:6238857-6238876 None:intergenic 65.0%
TCACGCCGGTCAATCACCGG+CGG + chr2.4:6238801-6238820 MS.gene31121:CDS 65.0%
ATCGCCGCCTTTGACGCCGG+TGG - chr2.4:6239168-6239187 None:intergenic 70.0%
CGCAGACGTCGTAACGCCGG+CGG + chr2.4:6238874-6238893 MS.gene31121:CDS 70.0%
CGCCGCAGACGTCGTAACGC+CGG + chr2.4:6238871-6238890 MS.gene31121:CDS 70.0%
CGCCGGCGTTACGACGTCTG+CGG - chr2.4:6238876-6238895 None:intergenic 70.0%
CGGCGTTACGACGTCTGCGG+CGG - chr2.4:6238873-6238892 None:intergenic 70.0%
GAAGACACCACCGCCGGTGC+CGG + chr2.4:6238943-6238962 MS.gene31121:CDS 70.0%
GGCGTTACGACGTCTGCGGC+GGG - chr2.4:6238872-6238891 None:intergenic 70.0%
GTTACGACGTCTGCGGCGGG+AGG - chr2.4:6238869-6238888 None:intergenic 70.0%
TTCGCCGGTGGTCGGAGAGC+CGG + chr2.4:6239486-6239505 MS.gene31121:CDS 70.0%
!! CTCACCGGCTCTCCGACCAC+CGG - chr2.4:6239493-6239512 None:intergenic 70.0%
! ACCGCCGCCGGCGTCTGCAC+CGG - chr2.4:6238965-6238984 None:intergenic 80.0%
! GCAGACGCCGGCGGCGGTAG+CGG + chr2.4:6238967-6238986 MS.gene31121:CDS 80.0%
!! GCCGGCGTCTGCACCGGCAC+CGG - chr2.4:6238959-6238978 None:intergenic 80.0%
!! GGCGTCTGCACCGGCACCGG+CGG - chr2.4:6238956-6238975 None:intergenic 80.0%
!!! GCCGGTGCCGGTGCAGACGC+CGG + chr2.4:6238955-6238974 MS.gene31121:CDS 80.0%
!!! GGTGCCGGTGCAGACGCCGG+CGG + chr2.4:6238958-6238977 MS.gene31121:CDS 80.0%
!!! GTCTGCACCGGCACCGGCGG+TGG - chr2.4:6238953-6238972 None:intergenic 80.0%
! GCCGGTGCAGACGCCGGCGG+CGG + chr2.4:6238961-6238980 MS.gene31121:CDS 85.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 6238670 6239960 6238670 ID=MS.gene31121
chr2.4 mRNA 6238670 6239960 6238670 ID=MS.gene31121.t1;Parent=MS.gene31121
chr2.4 exon 6238670 6239594 6238670 ID=MS.gene31121.t1.exon1;Parent=MS.gene31121.t1
chr2.4 CDS 6238670 6239594 6238670 ID=cds.MS.gene31121.t1;Parent=MS.gene31121.t1
chr2.4 exon 6239866 6239960 6239866 ID=MS.gene31121.t1.exon2;Parent=MS.gene31121.t1
chr2.4 CDS 6239866 6239960 6239866 ID=cds.MS.gene31121.t1;Parent=MS.gene31121.t1
Gene Sequence

>MS.gene31121

ATGAACTCGGAAACTGAAACACCATCCTCAACGACGTCGTTTTGGTGCTACAGCTGCACGCGTTTCATTCACATCTTGAACCAAAACAACGTCGTTTGTCCTCACTGCCAAAACGGATTCGTCGAAGAGATTCACGCCGGTCAATCACCGGCGGTTTCTCTTTTCGCCGACGGTATTCAGTCCTCCAGAAGACAAACCTCCCGCCGCAGACGTCGTAACGCCGGCGGCAGCCGTTCGCCTTTTAATCCTGTAATCGTTCTCCGTGGACCTAGAGAAGACACCACCGCCGGTGCCGGTGCAGACGCCGGCGGCGGTAGCGGTGTTGATAGCGAGGGGAATACAAGTAGTTTTGAATTATACTATGAAGACAGTAACGGTACCGATCTACGTCCACTTCCACCAAGCATGTCGGAGCTATTACTCGGTTCCGGCTTCGATCGGTTGTTAGAGCAATTCTCTCAAATCGAAATCAACGGTTTCGGAAGGTCGGAGAATCCACCGGCGTCAAAGGCGGCGATAGAGTCAATACCAACAGTTGAAATCACCGATTCAGAAATCGAATCCGAAATTCACTGTGCAGTTTGCAAAGAACAATTCGAGCTAGGTTCTGAAGCGCGTAAGATGCCGTGTAATCATCTTTACCATTCCGATTGCATTCTCCCATGGCTTTCAATGCGAAATTCGTGTCCGGTGTGTCGGCACGAGTTACCGTCCGATCAAAACGCGTCTGAGAGTAGAATTTCCGGTCAGATTGATGAAGAAGCGGTTGGATTGACGATATGGAGGTTACCGGGAGGTGGATTTGCTGTTGGAAGATTCGCCGGTGGTCGGAGAGCCGGTGAGATTCAGTTACCGATTGTTTACACGGAGATGGATGGTAACAATTCAAACGAAGATTCTAGAAGAATTTCTTTGACTGTGGGAAGTAATAGAGTTAGAGAGAGACGTGGAATTCGGAGAATTTTTCGAAATTTTTTATCGTTTTTTGGGAATGTCAACTTAAATCGTAGCTTTTCTGGTTCGTTGTTTAGTAGAAGCTCAAGAAGAAGAAGCAGAACTTGGGTTACAGAAGAATAATATGATTTGGTTTTGTTTGGATTAAAACTTGAAAGCTAGTTATTCATTTAAATTTAAAATTCTTACTGTAAATAGCATAAAATTGTGCTATAATTAAGTTTAGTAGATGATTATTGCAGGGTGCAAGATAAGGATTGTTGTTCTTGCTGATCAACAAACATACATAGTTGGGAAAGAAGCTAAGCTATGCTTATTTAGTGTTTATCGTTGTTAA
Protein sequence

>MS.gene31121.t1

MNSETETPSSTTSFWCYSCTRFIHILNQNNVVCPHCQNGFVEEIHAGQSPAVSLFADGIQSSRRQTSRRRRRNAGGSRSPFNPVIVLRGPREDTTAGAGADAGGGSGVDSEGNTSSFELYYEDSNGTDLRPLPPSMSELLLGSGFDRLLEQFSQIEINGFGRSENPPASKAAIESIPTVEITDSEIESEIHCAVCKEQFELGSEARKMPCNHLYHSDCILPWLSMRNSCPVCRHELPSDQNASESRISGQIDEEAVGLTIWRLPGGGFAVGRFAGGRRAGEIQLPIVYTEMDGNNSNEDSRRISLTVGRCKIRIVVLADQQTYIVGKEAKLCLFSVYRC