AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31413

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31413.t1 MTR_2g090865 99.535 215 1 0 1 215 1 215 7.09e-159 438
MS.gene31413.t1 MTR_4g088090 81.019 216 38 2 1 213 1 216 1.40e-128 361
MS.gene31413.t1 MTR_2g014990 80.556 216 39 2 1 213 1 216 2.26e-128 361
MS.gene31413.t1 MTR_4g079350 81.019 216 38 2 1 213 1 216 3.59e-128 360
MS.gene31413.t1 MTR_2g075950 76.415 212 47 3 5 214 7 217 6.58e-118 334
MS.gene31413.t1 MTR_2g083860 72.170 212 55 2 5 213 7 217 9.09e-108 308
MS.gene31413.t1 MTR_3g069510 67.453 212 66 2 4 213 5 215 3.59e-102 294
MS.gene31413.t1 MTR_2g005510 66.038 212 69 1 5 213 9 220 2.25e-101 293
MS.gene31413.t1 MTR_4g064897 67.136 213 66 3 4 214 5 215 7.60e-101 291
MS.gene31413.t1 MTR_1g052475 58.065 217 85 2 2 212 6 222 2.89e-89 262
MS.gene31413.t1 MTR_6g079550 58.768 211 84 3 5 213 6 215 2.19e-87 257
MS.gene31413.t1 MTR_3g086980 55.814 215 91 1 2 212 7 221 5.23e-86 254
MS.gene31413.t1 MTR_8g089985 56.881 218 89 2 2 214 8 225 1.80e-85 252
MS.gene31413.t1 MTR_0262s0050 55.814 215 90 2 2 212 6 219 2.78e-85 252
MS.gene31413.t1 MTR_8g027220 55.140 214 93 1 4 214 19 232 8.32e-81 241
MS.gene31413.t1 MTR_7g405790 58.937 207 82 2 10 213 14 220 6.09e-79 236
MS.gene31413.t1 MTR_1g078200 55.189 212 88 2 9 213 12 223 8.76e-77 230
MS.gene31413.t1 MTR_8g036220 60.221 181 71 1 2 181 6 186 2.39e-75 227
MS.gene31413.t1 MTR_7g051940 56.373 204 89 0 10 213 12 215 6.99e-75 225
MS.gene31413.t1 MTR_8g107070 56.522 207 86 2 5 208 8 213 1.34e-74 226
MS.gene31413.t1 MTR_7g117910 48.598 214 89 4 6 214 15 212 6.67e-65 200
MS.gene31413.t1 MTR_2g027660 46.009 213 104 2 8 213 3 211 1.98e-61 191
MS.gene31413.t1 MTR_6g021790 43.868 212 110 2 8 213 3 211 2.32e-58 183
MS.gene31413.t1 MTR_7g081700 45.498 211 106 1 4 214 1 202 6.52e-57 179
MS.gene31413.t1 MTR_8g031660 46.698 212 99 3 8 213 3 206 1.31e-55 176
MS.gene31413.t1 MTR_8g006970 47.087 206 101 2 9 214 20 217 2.15e-54 173
MS.gene31413.t1 MTR_2g005500 44.928 207 104 2 7 212 5 202 5.01e-54 172
MS.gene31413.t1 MTR_8g031870 53.165 158 74 0 10 167 5 162 1.09e-52 168
MS.gene31413.t1 MTR_8g006970 52.071 169 81 0 4 172 1 169 3.15e-52 166
MS.gene31413.t1 MTR_1g068730 42.453 212 112 3 7 214 11 216 1.35e-51 166
MS.gene31413.t1 MTR_7g100020 42.991 214 108 4 7 214 11 216 2.42e-51 165
MS.gene31413.t1 MTR_1g115325 42.308 208 114 3 7 212 11 214 6.89e-51 164
MS.gene31413.t1 MTR_1g068740 41.981 212 113 3 7 214 11 216 7.25e-51 164
MS.gene31413.t1 MTR_8g031680 40.865 208 121 1 8 213 3 210 3.33e-50 162
MS.gene31413.t1 MTR_8g093740 46.190 210 101 7 6 212 8 208 2.52e-49 160
MS.gene31413.t1 MTR_2g096550 40.826 218 109 5 3 212 7 212 8.80e-48 156
MS.gene31413.t1 MTR_8g031830 49.375 160 81 0 10 169 5 164 1.04e-47 155
MS.gene31413.t1 MTR_1g029450 45.455 209 105 6 7 213 13 214 1.07e-47 156
MS.gene31413.t1 MTR_3g096350 43.925 214 106 6 3 212 4 207 1.36e-46 153
MS.gene31413.t1 MTR_4g063160 41.315 213 110 5 7 212 11 215 2.02e-46 153
MS.gene31413.t1 MTR_1g094220 44.720 161 88 1 13 172 33 193 2.37e-45 149
MS.gene31413.t1 MTR_1g094220 44.937 158 86 1 13 169 33 190 8.09e-45 148
MS.gene31413.t1 MTR_3g064390 43.125 160 91 0 13 172 12 171 9.44e-45 148
MS.gene31413.t1 MTR_1g094220 46.104 154 82 1 13 165 33 186 2.71e-44 147
MS.gene31413.t1 MTR_1g094220 44.512 164 89 2 13 175 33 195 5.29e-44 147
MS.gene31413.t1 MTR_5g076590 42.500 160 92 0 13 172 12 171 1.89e-43 145
MS.gene31413.t1 MTR_4g099470 42.775 173 90 1 42 214 15 178 7.72e-43 142
MS.gene31413.t1 MTR_7g103070 42.788 208 111 4 8 213 13 214 1.15e-42 143
MS.gene31413.t1 MTR_1g068730 53.659 123 57 0 6 128 10 132 1.31e-42 142
MS.gene31413.t1 MTR_1g068740 53.659 123 57 0 6 128 10 132 2.66e-42 142
MS.gene31413.t1 MTR_1g090717 42.453 212 111 5 5 213 9 212 9.87e-42 140
MS.gene31413.t1 MTR_1g062760 43.478 207 108 5 8 212 11 210 1.26e-41 140
MS.gene31413.t1 MTR_2g102060 36.634 202 115 2 13 214 12 200 3.30e-41 139
MS.gene31413.t1 MTR_3g087160 33.333 195 112 1 12 206 10 186 9.93e-39 133
MS.gene31413.t1 MTR_8g103550 39.375 160 97 0 13 172 11 170 1.75e-38 132
MS.gene31413.t1 MTR_4g098950 33.178 214 113 3 12 213 10 205 1.94e-38 132
MS.gene31413.t1 MTR_5g013900 34.694 196 109 2 12 206 10 187 4.67e-38 131
MS.gene31413.t1 MTR_8g090215 35.057 174 113 0 12 185 10 183 6.66e-38 130
MS.gene31413.t1 MTR_1g097420 40.964 166 89 4 11 168 8 172 5.93e-36 125
MS.gene31413.t1 MTR_3g096350 52.344 128 56 4 3 127 4 129 1.55e-35 123
MS.gene31413.t1 MTR_8g092020 39.759 166 91 4 11 168 12 176 4.78e-35 123
MS.gene31413.t1 MTR_3g096350 52.344 128 56 4 3 127 4 129 5.08e-35 122
MS.gene31413.t1 MTR_4g069850 34.928 209 116 7 11 211 8 204 2.57e-34 121
MS.gene31413.t1 MTR_2g101890 39.286 168 94 4 11 171 10 176 3.56e-34 121
MS.gene31413.t1 MTR_4g012940 39.506 162 91 3 11 165 8 169 1.94e-33 119
MS.gene31413.t1 MTR_3g064390 44.000 125 70 0 13 137 12 136 3.22e-33 117
MS.gene31413.t1 MTR_8g093740 43.678 174 87 6 42 212 10 175 1.28e-32 116
MS.gene31413.t1 MTR_1g069245 64.211 95 30 2 24 118 109 199 5.56e-32 116
MS.gene31413.t1 MTR_6g039490 37.576 165 96 3 11 168 8 172 5.86e-32 116
MS.gene31413.t1 MTR_1g097420 38.095 168 93 5 9 168 35 199 5.81e-30 111
MS.gene31413.t1 MTR_7g103070 42.405 158 83 4 58 213 7 158 3.63e-29 107
MS.gene31413.t1 MTR_2g005500 32.850 207 89 3 7 212 5 162 1.23e-25 98.2
MS.gene31413.t1 MTR_3g107717 32.258 155 102 2 12 166 14 165 1.14e-23 94.4
MS.gene31413.t1 MTR_3g107713 32.258 155 102 2 12 166 14 165 1.38e-23 94.4
MS.gene31413.t1 MTR_3g107707 32.258 155 102 2 12 166 14 165 1.39e-23 94.4
MS.gene31413.t1 MTR_2g078000 81.633 49 9 0 72 120 46 94 1.47e-23 90.9
MS.gene31413.t1 MTR_8g031810 43.689 103 44 1 10 112 5 93 6.18e-21 84.0
MS.gene31413.t1 MTR_4g113620 30.968 155 95 4 12 166 14 156 5.82e-19 81.6
MS.gene31413.t1 MTR_2g078560 48.980 98 40 2 73 164 54 147 1.25e-18 82.8
MS.gene31413.t1 MTR_1452s0010 41.772 79 46 0 31 109 1 79 3.53e-18 77.0
MS.gene31413.t1 MTR_4g073250 31.282 195 115 7 11 190 6 196 6.40e-17 75.9
MS.gene31413.t1 MTR_5g022600 37.500 120 72 3 11 129 6 123 6.74e-17 75.9
MS.gene31413.t1 MTR_4g088055 37.500 120 72 3 11 129 6 123 1.07e-16 75.5
MS.gene31413.t1 MTR_2g090875 37.500 120 72 3 11 129 6 123 1.50e-16 75.1
MS.gene31413.t1 MTR_8g075240 37.500 120 72 3 11 129 6 123 2.46e-16 74.3
MS.gene31413.t1 MTR_3g078260 31.138 167 101 5 11 165 8 172 4.23e-16 74.3
MS.gene31413.t1 MTR_6g087980 36.364 121 74 3 11 130 6 124 1.03e-15 73.2
MS.gene31413.t1 MTR_3g053480 62.500 48 18 0 6 53 11 58 6.81e-15 67.4
MS.gene31413.t1 MTR_1686s0010 33.696 92 61 0 31 122 1 92 1.14e-14 67.4
MS.gene31413.t1 MTR_8g099250 64.583 48 17 0 6 53 11 58 3.95e-14 65.1
MS.gene31413.t1 MTR_1g059590 31.737 167 101 5 8 171 395 551 2.73e-13 68.6
MS.gene31413.t1 MTR_1g059590 31.737 167 101 5 8 171 428 584 3.05e-13 68.2
MS.gene31413.t1 MTR_3g078260 34.483 116 73 3 11 125 8 121 3.55e-13 64.7
MS.gene31413.t1 MTR_5g092060 24.852 169 121 2 12 175 105 272 1.55e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31413.t1 AT5G60860 81.429 210 38 1 5 213 7 216 8.27e-127 357
MS.gene31413.t1 AT3G15060 78.199 211 45 1 5 214 7 217 4.72e-123 347
MS.gene31413.t1 AT4G18430 76.852 216 47 2 1 213 1 216 3.25e-122 345
MS.gene31413.t1 AT1G28550 76.498 217 47 3 1 213 1 217 7.17e-122 344
MS.gene31413.t1 AT2G33870 76.415 212 48 2 5 214 7 218 9.83e-120 339
MS.gene31413.t1 AT5G45750 77.619 210 45 2 5 213 7 215 1.24e-118 336
MS.gene31413.t1 AT4G18800 77.143 210 44 2 5 213 7 213 5.09e-117 332
MS.gene31413.t1 AT1G16920 73.460 211 54 2 4 213 6 215 7.96e-113 322
MS.gene31413.t1 AT1G06400 73.333 210 54 2 5 213 7 215 8.13e-113 322
MS.gene31413.t1 AT1G09630 65.728 213 70 2 5 215 6 217 1.16e-103 298
MS.gene31413.t1 AT1G07410 64.623 212 72 2 4 214 5 214 2.38e-100 290
MS.gene31413.t1 AT5G59150 64.186 215 71 3 4 214 5 217 9.32e-96 278
MS.gene31413.t1 AT3G46830 63.721 215 72 3 4 214 5 217 1.37e-95 278
MS.gene31413.t1 AT3G46830 63.721 215 72 3 4 214 5 217 1.37e-95 278
MS.gene31413.t1 AT5G47960 58.796 216 83 3 5 215 9 223 7.06e-88 258
MS.gene31413.t1 AT3G12160 57.209 215 87 2 2 212 6 219 1.47e-87 258
MS.gene31413.t1 AT4G39990 57.143 210 87 1 9 215 15 224 6.55e-85 251
MS.gene31413.t1 AT5G65270 55.708 219 91 2 2 214 8 226 1.62e-84 250
MS.gene31413.t1 AT5G47520 56.542 214 89 1 5 214 8 221 3.96e-82 244
MS.gene31413.t1 AT1G18200 53.812 223 86 3 5 212 7 227 6.23e-80 239
MS.gene31413.t1 AT1G05810 58.373 209 83 2 9 214 10 217 1.48e-78 234
MS.gene31413.t1 AT1G73640 55.556 207 85 2 5 204 7 213 3.78e-78 234
MS.gene31413.t1 AT1G05810 58.373 209 83 2 9 214 56 263 3.96e-78 235
MS.gene31413.t1 AT3G07410 57.407 216 89 2 1 213 1 216 4.86e-78 233
MS.gene31413.t1 AT2G31680 55.714 210 90 1 9 215 10 219 7.21e-78 233
MS.gene31413.t1 AT2G43130 58.173 208 80 2 9 213 10 213 8.22e-78 233
MS.gene31413.t1 AT1G01200 55.093 216 91 4 4 213 21 236 2.81e-77 232
MS.gene31413.t1 AT5G59150 58.434 166 63 3 53 214 3 166 5.54e-63 193
MS.gene31413.t1 AT4G17170 46.009 213 104 2 8 213 3 211 1.17e-61 191
MS.gene31413.t1 AT4G35860 44.976 209 112 1 8 213 3 211 2.15e-59 186
MS.gene31413.t1 AT4G17160 45.411 207 106 2 8 212 3 204 5.04e-58 182
MS.gene31413.t1 AT1G02130 46.445 211 105 3 4 214 1 203 9.55e-57 179
MS.gene31413.t1 AT4G17530 46.445 211 104 3 4 214 1 202 2.08e-55 175
MS.gene31413.t1 AT5G47200 45.116 215 101 3 4 214 1 202 4.22e-55 174
MS.gene31413.t1 AT3G11730 44.712 208 105 3 7 212 4 203 1.55e-53 171
MS.gene31413.t1 AT5G03520 45.755 212 102 4 7 213 11 214 6.69e-53 169
MS.gene31413.t1 AT3G46060 43.458 214 107 4 7 214 11 216 4.27e-51 165
MS.gene31413.t1 AT3G46060 43.458 214 107 4 7 214 11 216 4.27e-51 165
MS.gene31413.t1 AT3G46060 43.458 214 107 4 7 214 11 216 4.27e-51 165
MS.gene31413.t1 AT3G53610 42.523 214 109 4 7 214 11 216 1.17e-50 164
MS.gene31413.t1 AT3G53610 42.523 214 109 4 7 214 11 216 1.17e-50 164
MS.gene31413.t1 AT3G53610 42.523 214 109 4 7 214 11 216 1.17e-50 164
MS.gene31413.t1 AT3G09900 43.925 214 105 4 7 213 11 216 1.24e-50 164
MS.gene31413.t1 AT5G59840 42.523 214 109 4 7 214 11 216 4.96e-50 162
MS.gene31413.t1 AT5G45130 40.299 201 108 4 13 213 12 200 5.44e-47 154
MS.gene31413.t1 AT1G43890 47.701 174 88 3 6 177 8 180 4.38e-46 152
MS.gene31413.t1 AT1G43890 47.701 174 88 3 6 177 8 180 4.38e-46 152
MS.gene31413.t1 AT1G43890 47.701 174 88 3 6 177 8 180 4.38e-46 152
MS.gene31413.t1 AT3G54840 47.134 157 82 1 13 168 35 191 6.07e-46 151
MS.gene31413.t1 AT3G54840 46.795 156 82 1 14 168 2 157 1.66e-45 149
MS.gene31413.t1 AT5G45130 39.698 199 108 4 15 213 22 208 2.51e-45 150
MS.gene31413.t1 AT3G54840 47.403 154 80 1 13 165 35 188 3.53e-45 149
MS.gene31413.t1 AT4G19640 42.331 163 94 0 13 175 12 174 4.11e-45 149
MS.gene31413.t1 AT5G03530 44.231 208 107 5 8 213 10 210 2.42e-44 147
MS.gene31413.t1 AT5G03530 44.231 208 107 5 8 213 10 210 2.42e-44 147
MS.gene31413.t1 AT4G35860 41.463 164 93 1 53 213 2 165 1.94e-40 136
MS.gene31413.t1 AT5G64990 37.267 161 101 0 12 172 8 168 2.46e-38 132
MS.gene31413.t1 AT2G44610 33.333 216 112 3 12 213 10 207 5.59e-38 131
MS.gene31413.t1 AT2G22290 33.168 202 130 2 12 213 10 206 5.62e-38 131
MS.gene31413.t1 AT1G22740 42.515 167 85 5 11 168 8 172 9.51e-38 130
MS.gene31413.t1 AT5G03520 42.197 173 87 4 46 213 40 204 1.68e-37 130
MS.gene31413.t1 AT5G10260 36.943 157 99 0 12 168 10 166 9.76e-37 128
MS.gene31413.t1 AT4G39890 36.364 165 104 1 12 175 10 174 1.31e-36 127
MS.gene31413.t1 AT4G09720 39.394 165 93 3 11 168 8 172 8.63e-36 125
MS.gene31413.t1 AT5G64990 35.714 168 101 1 12 172 8 175 1.21e-35 125
MS.gene31413.t1 AT3G09910 42.781 187 100 4 8 192 10 191 3.36e-35 124
MS.gene31413.t1 AT3G09910 42.781 187 100 4 8 192 10 191 3.36e-35 124
MS.gene31413.t1 AT3G09910 42.781 187 100 4 8 192 10 191 3.36e-35 124
MS.gene31413.t1 AT4G19640 43.077 130 74 0 13 142 12 141 3.58e-35 122
MS.gene31413.t1 AT5G46025 58.491 106 43 1 73 177 6 111 3.74e-35 120
MS.gene31413.t1 AT2G21880 40.000 165 92 3 11 168 9 173 4.18e-35 124
MS.gene31413.t1 AT1G52280 40.237 169 91 5 11 171 8 174 9.91e-35 122
MS.gene31413.t1 AT4G19640 42.748 131 75 0 13 143 12 142 1.09e-34 122
MS.gene31413.t1 AT4G09720 38.824 170 92 4 11 168 8 177 3.24e-34 121
MS.gene31413.t1 AT3G18820 39.506 162 91 3 11 165 8 169 1.38e-33 119
MS.gene31413.t1 AT3G09910 43.114 167 92 3 8 172 10 175 1.82e-33 119
MS.gene31413.t1 AT3G09910 43.114 167 92 3 8 172 10 175 1.82e-33 119
MS.gene31413.t1 AT4G09720 37.500 176 92 4 11 168 8 183 3.45e-33 119
MS.gene31413.t1 AT3G16100 39.264 163 90 4 11 165 8 169 6.25e-33 118
MS.gene31413.t1 AT1G49300 37.725 167 97 3 11 170 8 174 1.91e-32 117
MS.gene31413.t1 AT1G49300 37.725 167 97 3 11 170 8 174 1.91e-32 117
MS.gene31413.t1 AT5G39620 38.415 164 94 3 12 168 6 169 3.30e-32 116
MS.gene31413.t1 AT2G21880 37.838 148 85 3 28 168 1 148 2.36e-26 100
MS.gene31413.t1 AT2G21880 37.838 148 85 3 28 168 1 148 2.36e-26 100
MS.gene31413.t1 AT4G09720 39.130 138 77 3 38 168 1 138 2.48e-25 97.4
MS.gene31413.t1 AT4G09720 39.130 138 77 3 38 168 1 138 2.48e-25 97.4
MS.gene31413.t1 AT4G09720 39.130 138 77 3 38 168 1 138 2.48e-25 97.4
MS.gene31413.t1 AT2G21880 35.152 165 92 4 11 168 9 165 3.60e-25 98.2
MS.gene31413.t1 AT5G20020 32.258 155 102 2 12 166 14 165 1.27e-23 94.4
MS.gene31413.t1 AT5G55190 32.258 155 102 2 12 166 14 165 1.43e-23 94.4
MS.gene31413.t1 AT5G20010 32.258 155 102 2 12 166 14 165 1.50e-23 94.4
MS.gene31413.t1 AT5G55080 30.435 161 109 2 12 172 14 171 2.68e-23 93.6
MS.gene31413.t1 AT2G44690 32.222 180 108 5 11 178 18 195 2.34e-19 82.8
MS.gene31413.t1 AT4G08190 50.704 71 32 1 95 165 60 127 6.55e-18 77.0
MS.gene31413.t1 AT4G35020 34.667 150 88 4 11 159 6 146 2.69e-17 77.0
MS.gene31413.t1 AT4G35020 34.667 150 88 4 11 159 6 146 2.69e-17 77.0
MS.gene31413.t1 AT4G35020 34.667 150 88 4 11 159 6 146 2.69e-17 77.0
MS.gene31413.t1 AT1G75840 37.500 120 72 3 11 129 6 123 1.74e-16 75.1
MS.gene31413.t1 AT1G20090 36.667 120 73 3 11 129 5 122 2.87e-16 74.3
MS.gene31413.t1 AT3G51300 38.136 118 70 3 13 129 8 123 3.52e-16 73.9
MS.gene31413.t1 AT2G17800 37.500 120 72 3 11 129 6 123 3.52e-16 73.9
MS.gene31413.t1 AT2G17800 37.500 120 72 3 11 129 6 123 3.52e-16 73.9
MS.gene31413.t1 AT4G35950 37.500 120 72 3 11 129 6 123 3.67e-16 73.9
MS.gene31413.t1 AT3G48040 31.737 167 100 5 11 165 8 172 4.96e-16 73.9
MS.gene31413.t1 AT4G28950 36.667 120 73 3 11 129 6 123 6.81e-16 73.6
MS.gene31413.t1 AT5G45970 36.667 120 73 3 11 129 6 123 8.22e-16 73.2
MS.gene31413.t1 AT5G62880 31.461 178 104 6 11 172 8 183 1.22e-15 73.2
MS.gene31413.t1 AT3G48040 36.667 120 73 3 11 129 8 125 2.51e-15 71.2
MS.gene31413.t1 AT5G62880 35.833 120 74 3 11 129 8 125 5.73e-15 70.9
MS.gene31413.t1 AT4G35020 36.066 122 75 3 13 133 8 127 3.32e-14 67.8
MS.gene31413.t1 AT4G35020 36.066 122 75 3 13 133 8 127 3.32e-14 67.8

Find 36 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAACGCCGATGGTGGATT+TGG 0.266153 2.4:-9623492 None:intergenic
TGGAGCTGTTGGCGCTTTGC+TGG 0.307634 2.4:+9624744 MS.gene31413:CDS
GCTTGTTAAGGCTCAGATTT+GGG 0.349305 2.4:+9623547 MS.gene31413:CDS
AGCTTGTTAAGGCTCAGATT+TGG 0.356509 2.4:+9623546 MS.gene31413:CDS
GAAAGAAAACATATATTTCA+TGG 0.359463 2.4:+9624930 MS.gene31413:CDS
GATTCTTGATAAGCTTGTTA+AGG 0.421134 2.4:+9623535 MS.gene31413:CDS
AAGGGAGAGACTATCAATAT+TGG 0.429829 2.4:+9625072 MS.gene31413:CDS
CACTAGAAATGAATTCAACT+TGG 0.437767 2.4:+9623466 MS.gene31413:CDS
AATTTGCTACTCGAAGTGTT+AGG 0.449148 2.4:+9623513 MS.gene31413:CDS
TGGCTCTGCATCAGTGCCTA+AGG 0.468622 2.4:+9625053 MS.gene31413:CDS
TATGTTGTAATCATGCTTGT+AGG 0.477521 2.4:+9624844 MS.gene31413:CDS
TTGATCGGTGATTCTGGTGT+TGG 0.493431 2.4:+9623419 MS.gene31413:CDS
AGTGAATCTAGATAAAAGAT+TGG 0.496154 2.4:-9623447 None:intergenic
TTCTTTCTCAGCAAATGTGG+TGG 0.512980 2.4:-9624914 None:intergenic
AAGACTCTTGAGAAAGAGAA+TGG 0.519287 2.4:+9625033 MS.gene31413:CDS
AGCAAATTCAACGCCGATGG+TGG 0.527864 2.4:-9623498 None:intergenic
GTTTCAGATGTTAAGAAGAG+TGG 0.531135 2.4:+9625105 MS.gene31413:CDS
GGGATACTGCTGGTCAAGAA+AGG 0.539209 2.4:+9623567 MS.gene31413:CDS
ATACGATTACTTGTTCAAGT+TGG 0.541653 2.4:+9623394 MS.gene31413:CDS
AAATGTTGAGAGGTGGTTGA+AGG 0.552257 2.4:+9624798 MS.gene31413:CDS
ATATTGATAGTCTCTCCCTT+AGG 0.560011 2.4:-9625069 None:intergenic
GCATACTACCGTGGAGCTGT+TGG 0.560757 2.4:+9624733 MS.gene31413:CDS
GGTGGCTTCTTCCGTTGACA+CGG 0.568498 2.4:-9624896 None:intergenic
GCTCAGATTTGGGATACTGC+TGG 0.571910 2.4:+9623557 MS.gene31413:CDS
TACAAGCATGATTACAACAT+AGG 0.576438 2.4:-9624842 None:intergenic
TGCACTAGAGTCGTTGAATG+TGG 0.582071 2.4:+9624960 MS.gene31413:CDS
GAATGTGGACAATGCTTTCG+TGG 0.585079 2.4:+9624975 MS.gene31413:CDS
GGCTCTGCATCAGTGCCTAA+GGG 0.588553 2.4:+9625054 MS.gene31413:CDS
ACACTTGCAAGCCGTGTCAA+CGG 0.594812 2.4:+9624885 MS.gene31413:CDS
CATTTGAAAATGTTGAGAGG+TGG 0.614736 2.4:+9624791 MS.gene31413:CDS
AGTAGCAAATTCAACGCCGA+TGG 0.615075 2.4:-9623501 None:intergenic
TTGGAATCCAAATCCACCAT+CGG 0.626401 2.4:+9623485 MS.gene31413:CDS
TGCTGGTGTACGACTCAACA+AGG 0.627995 2.4:+9624761 MS.gene31413:CDS
GCAAAGCGCCAACAGCTCCA+CGG 0.659685 2.4:-9624741 None:intergenic
TGACATTTGAAAATGTTGAG+AGG 0.673555 2.4:+9624788 MS.gene31413:CDS
ATCACAAGTGCATACTACCG+TGG 0.721157 2.4:+9624724 MS.gene31413:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAACTTGTTATATTGTTTT+TGG + chr2.4:9624305-9624324 MS.gene31413:intron 15.0%
!! ATATTGGAAGAAATAAACAA+TGG - chr2.4:9624108-9624127 None:intergenic 20.0%
!! GAAAGAAAACATATATTTCA+TGG + chr2.4:9624930-9624949 MS.gene31413:CDS 20.0%
!! GTAATTTCTTCAATATATCA+AGG + chr2.4:9624150-9624169 MS.gene31413:intron 20.0%
!! TCTTAATATTGTAGAGAATT+GGG + chr2.4:9624033-9624052 MS.gene31413:intron 20.0%
!! TTCTTAATATTGTAGAGAAT+TGG + chr2.4:9624032-9624051 MS.gene31413:intron 20.0%
!!! CAAATTTTGAAAAAGCTAAA+TGG - chr2.4:9624131-9624150 None:intergenic 20.0%
!!! TGAGATTATTTTAACTTTAG+AGG - chr2.4:9624339-9624358 None:intergenic 20.0%
! AGAATGAAATTGAAGAGAAA+AGG - chr2.4:9623598-9623617 None:intergenic 25.0%
! AGTGAATCTAGATAAAAGAT+TGG - chr2.4:9623450-9623469 None:intergenic 25.0%
! GATTGATGAATTGAATTGAA+TGG + chr2.4:9623785-9623804 MS.gene31413:intron 25.0%
! GTTCAATCTGTTGTTAAATA+AGG + chr2.4:9623967-9623986 MS.gene31413:intron 25.0%
! TGAAAAAGCTAAATGGATAT+TGG - chr2.4:9624124-9624143 None:intergenic 25.0%
! TGATATGATCACAAAAACTA+TGG + chr2.4:9624068-9624087 MS.gene31413:intron 25.0%
!! AAATGGAATTCTCATTTTAG+AGG - chr2.4:9624581-9624600 None:intergenic 25.0%
!! AAATTGAACTCTCATTTTAG+AGG - chr2.4:9624464-9624483 None:intergenic 25.0%
!!! GTATCTTAGAATTTGTTGTT+GGG + chr2.4:9623884-9623903 MS.gene31413:intron 25.0%
!!! TACTCTTTCATTTTTAATGC+CGG + chr2.4:9623943-9623962 MS.gene31413:intron 25.0%
!!! TACTTTTTTGCTTTTGATCT+TGG + chr2.4:9623829-9623848 MS.gene31413:intron 25.0%
!!! TGTATCTTAGAATTTGTTGT+TGG + chr2.4:9623883-9623902 MS.gene31413:intron 25.0%
AAAACGAGTTTACGAGATTT+AGG - chr2.4:9624556-9624575 None:intergenic 30.0%
AATGGAGTGAAAATGCAAAA+GGG + chr2.4:9623803-9623822 MS.gene31413:intron 30.0%
AGACGGAAAAATTCTCTAAA+TGG - chr2.4:9624598-9624617 None:intergenic 30.0%
ATACGATTACTTGTTCAAGT+TGG + chr2.4:9623394-9623413 MS.gene31413:CDS 30.0%
ATGATCACAAAAACTATGGA+TGG + chr2.4:9624072-9624091 MS.gene31413:intron 30.0%
CACTAGAAATGAATTCAACT+TGG + chr2.4:9623466-9623485 MS.gene31413:CDS 30.0%
GGAAAAAGTAAGTGCATATT+TGG - chr2.4:9624443-9624462 None:intergenic 30.0%
GGAAGAAATAAACAATGGTT+AGG - chr2.4:9624103-9624122 None:intergenic 30.0%
TAAGTGCATATTTGGTATCA+CGG - chr2.4:9624435-9624454 None:intergenic 30.0%
TACAAGCATGATTACAACAT+AGG - chr2.4:9624845-9624864 None:intergenic 30.0%
TATGTTGTAATCATGCTTGT+AGG + chr2.4:9624844-9624863 MS.gene31413:CDS 30.0%
TGACATTTGAAAATGTTGAG+AGG + chr2.4:9624788-9624807 MS.gene31413:CDS 30.0%
TGTAAGTATCACATTGTAGA+CGG - chr2.4:9624615-9624634 None:intergenic 30.0%
!! ACTCTTTCATTTTTAATGCC+GGG + chr2.4:9623944-9623963 MS.gene31413:intron 30.0%
!! GACTTTTAGAAGAGAAACTA+TGG - chr2.4:9624271-9624290 None:intergenic 30.0%
!! GATTCTTGATAAGCTTGTTA+AGG + chr2.4:9623535-9623554 MS.gene31413:CDS 30.0%
!! GGGTTTTGATCTTAATGTTT+AGG + chr2.4:9623679-9623698 MS.gene31413:intron 30.0%
!! GTGTCTGATAATTTGTTGTT+GGG + chr2.4:9623727-9623746 MS.gene31413:intron 30.0%
!! TGTGTCTGATAATTTGTTGT+TGG + chr2.4:9623726-9623745 MS.gene31413:intron 30.0%
!!! AGAGTTTTAGAAGACAATCT+AGG - chr2.4:9624206-9624225 None:intergenic 30.0%
!!! AGTGTGTTTTTTTGTGGTTT+TGG + chr2.4:9623658-9623677 MS.gene31413:intron 30.0%
!!! AGTTTTAGAAGACAATCTAG+GGG - chr2.4:9624204-9624223 None:intergenic 30.0%
!!! GAGTTTTAGAAGACAATCTA+GGG - chr2.4:9624205-9624224 None:intergenic 30.0%
!!! GCAAAAAGTGTGTTTTTTTG+TGG + chr2.4:9623652-9623671 MS.gene31413:intron 30.0%
!!! GTGTGTTTTTTTGTGGTTTT+GGG + chr2.4:9623659-9623678 MS.gene31413:intron 30.0%
!!! TTGTTCAAGTTGGTTTTGAT+CGG + chr2.4:9623404-9623423 MS.gene31413:CDS 30.0%
AAGACACTGATCATAAACAG+AGG + chr2.4:9623857-9623876 MS.gene31413:intron 35.0%
AAGACTCTTGAGAAAGAGAA+TGG + chr2.4:9625033-9625052 MS.gene31413:CDS 35.0%
AAGGGAGAGACTATCAATAT+TGG + chr2.4:9625072-9625091 MS.gene31413:CDS 35.0%
AATGAGAGACTACAAGATTC+AGG - chr2.4:9624676-9624695 None:intergenic 35.0%
ATCACAAAAACTATGGATGG+TGG + chr2.4:9624075-9624094 MS.gene31413:intron 35.0%
CATTTGAAAATGTTGAGAGG+TGG + chr2.4:9624791-9624810 MS.gene31413:CDS 35.0%
GAAGAGAAACTATGGCTAAT+TGG - chr2.4:9624263-9624282 None:intergenic 35.0%
GAATGGAGTGAAAATGCAAA+AGG + chr2.4:9623802-9623821 MS.gene31413:intron 35.0%
GTTTCAGATGTTAAGAAGAG+TGG + chr2.4:9625105-9625124 MS.gene31413:CDS 35.0%
TTTAACAACAGATTGAACCC+CGG - chr2.4:9623965-9623984 None:intergenic 35.0%
! ATATTGATAGTCTCTCCCTT+AGG - chr2.4:9625072-9625091 None:intergenic 35.0%
! GTTTTCTTTCTCAGCAAATG+TGG - chr2.4:9624920-9624939 None:intergenic 35.0%
!! AATTTGCTACTCGAAGTGTT+AGG + chr2.4:9623513-9623532 MS.gene31413:CDS 35.0%
!! AATTTGTTGTTGGGTAGAGA+AGG + chr2.4:9623736-9623755 MS.gene31413:intron 35.0%
!! CTCTTTCATTTTTAATGCCG+GGG + chr2.4:9623945-9623964 MS.gene31413:intron 35.0%
AAATGTTGAGAGGTGGTTGA+AGG + chr2.4:9624798-9624817 MS.gene31413:CDS 40.0%
GAAGACAATCTAGGGGTAAT+AGG - chr2.4:9624197-9624216 None:intergenic 40.0%
GTTGGGTAGAGAAGGAATTT+GGG + chr2.4:9623901-9623920 MS.gene31413:intron 40.0%
GTTGGGTAGAGAATGAATTG+AGG + chr2.4:9623744-9623763 MS.gene31413:intron 40.0%
TGTTGGGTAGAGAAGGAATT+TGG + chr2.4:9623900-9623919 MS.gene31413:intron 40.0%
TTCTTTCTCAGCAAATGTGG+TGG - chr2.4:9624917-9624936 None:intergenic 40.0%
TTGGAATCCAAATCCACCAT+CGG + chr2.4:9623485-9623504 MS.gene31413:CDS 40.0%
! GCTTGTTAAGGCTCAGATTT+GGG + chr2.4:9623547-9623566 MS.gene31413:CDS 40.0%
!! AGCTTGTTAAGGCTCAGATT+TGG + chr2.4:9623546-9623565 MS.gene31413:CDS 40.0%
!!! TTGGTTTTGATCGGTGATTC+TGG + chr2.4:9623413-9623432 MS.gene31413:CDS 40.0%
AAGATGTCCTGCTGCATGTT+TGG + chr2.4:9624378-9624397 MS.gene31413:intron 45.0%
AGTAGCAAATTCAACGCCGA+TGG - chr2.4:9623504-9623523 None:intergenic 45.0%
ATCACAAGTGCATACTACCG+TGG + chr2.4:9624724-9624743 MS.gene31413:CDS 45.0%
GAATGTGGACAATGCTTTCG+TGG + chr2.4:9624975-9624994 MS.gene31413:CDS 45.0%
TGCACTAGAGTCGTTGAATG+TGG + chr2.4:9624960-9624979 MS.gene31413:CDS 45.0%
TGTGATACCAAACATGCAGC+AGG - chr2.4:9624388-9624407 None:intergenic 45.0%
TTGATCGGTGATTCTGGTGT+TGG + chr2.4:9623419-9623438 MS.gene31413:CDS 45.0%
ACACTTGCAAGCCGTGTCAA+CGG + chr2.4:9624885-9624904 MS.gene31413:CDS 50.0%
AGCAAATTCAACGCCGATGG+TGG - chr2.4:9623501-9623520 None:intergenic 50.0%
GGGATACTGCTGGTCAAGAA+AGG + chr2.4:9623567-9623586 MS.gene31413:CDS 50.0%
TGCTGGTGTACGACTCAACA+AGG + chr2.4:9624761-9624780 MS.gene31413:CDS 50.0%
! GCTCAGATTTGGGATACTGC+TGG + chr2.4:9623557-9623576 MS.gene31413:CDS 50.0%
!! TTCAACGCCGATGGTGGATT+TGG - chr2.4:9623495-9623514 None:intergenic 50.0%
GCATACTACCGTGGAGCTGT+TGG + chr2.4:9624733-9624752 MS.gene31413:CDS 55.0%
GGCTCTGCATCAGTGCCTAA+GGG + chr2.4:9625054-9625073 MS.gene31413:CDS 55.0%
GGTGGCTTCTTCCGTTGACA+CGG - chr2.4:9624899-9624918 None:intergenic 55.0%
! TGGCTCTGCATCAGTGCCTA+AGG + chr2.4:9625053-9625072 MS.gene31413:CDS 55.0%
TGGAGCTGTTGGCGCTTTGC+TGG + chr2.4:9624744-9624763 MS.gene31413:CDS 60.0%
! GCAAAGCGCCAACAGCTCCA+CGG - chr2.4:9624744-9624763 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 9623374 9625146 9623374 ID=MS.gene31413
chr2.4 mRNA 9623374 9625146 9623374 ID=MS.gene31413.t1;Parent=MS.gene31413
chr2.4 exon 9623374 9623588 9623374 ID=MS.gene31413.t1.exon1;Parent=MS.gene31413.t1
chr2.4 CDS 9623374 9623588 9623374 ID=cds.MS.gene31413.t1;Parent=MS.gene31413.t1
chr2.4 exon 9624714 9625146 9624714 ID=MS.gene31413.t1.exon2;Parent=MS.gene31413.t1
chr2.4 CDS 9624714 9625146 9624714 ID=cds.MS.gene31413.t1;Parent=MS.gene31413.t1
Gene Sequence

>MS.gene31413

ATGGGTGCTGTAGATGAAGAATACGATTACTTGTTCAAGTTGGTTTTGATCGGTGATTCTGGTGTTGGAAAGTCCAATCTTTTATCTAGATTCACTAGAAATGAATTCAACTTGGAATCCAAATCCACCATCGGCGTTGAATTTGCTACTCGAAGTGTTAGGATTCTTGATAAGCTTGTTAAGGCTCAGATTTGGGATACTGCTGGTCAAGAAAGGTCACTCCTTTTCTCTTCAATTTCATTCTTTATGCTTAGTTTATGATCTGCTTTTGTTTGTTTGCAAAAAGTGTGTTTTTTTGTGGTTTTGGGTTTTGATCTTAATGTTTAGGACAGTGATCATAAACAGATGATTTTGTGTCTGATAATTTGTTGTTGGGTAGAGAATGAATTGAGGTTTAAGATTTTTGTTGTTGATTGATGAATTGAATTGAATGGAGTGAAAATGCAAAAGGGTGTTACTTTTTTGCTTTTGATCTTGGTGTTTAAGACACTGATCATAAACAGAGGAATTGTATCTTAGAATTTGTTGTTGGGTAGAGAAGGAATTTGGGTTTAAGAATGTCTAAGTGATACTCTTTCATTTTTAATGCCGGGGTTCAATCTGTTGTTAAATAAGGTTTTAAATTGTGATTGTGCTTGTAATTGCATTGTGATTGTGATTCTTAATATTGTAGAGAATTGGGATTAAATATCACTGATATGATCACAAAAACTATGGATGGTGGAGCCTAACCATTGTTTATTTCTTCCAATATCCATTTAGCTTTTTCAAAATTTGTAATTTCTTCAATATATCAAGGTTTTTGTTCAATATGATTGTTCCTATTACCCCTAGATTGTCTTCTAAAACTCTACTTCTAATCTTCAATTTGTTCAATATGATTGCTCCAATTAGCCATAGTTTCTCTTCTAAAAGTCTAGACTTTAAGTTTTAAACTTGTTATATTGTTTTTGGATCTAACTCCTCTAAAGTTAAAATAATCTCAACAGATGAAATCAACTATCAAGATGTCCTGCTGCATGTTTGGTATCACATTGAGTAATTCTGATGAACTCTCACCGTGATACCAAATATGCACTTACTTTTTCCTCTAAAATGAGAGTTCAATTTAGAGAATTTGTTCATTTACAATGTGATAATACTAATACTTATCAAAACTAGATAAATATATAACTCATTCCTAAATCTCGTAAACTCGTTTTTTCCTCTAAAATGAGAATTCCATTTAGAGAATTTTTCCGTCTACAATGTGATACTTACATGCTAATCAAACTTAGATAAATTATAGTATAACTCATTCCTGAATCTTGTAGTCTCTCATTCTTTTTAATGCATCACAGATATCGTGCAATCACAAGTGCATACTACCGTGGAGCTGTTGGCGCTTTGCTGGTGTACGACTCAACAAGGCATGTGACATTTGAAAATGTTGAGAGGTGGTTGAAGGAGCTTCGAGATCATACAGATGCCTATGTTGTAATCATGCTTGTAGGAAACAAAGCAGATCTTCGACACTTGCAAGCCGTGTCAACGGAAGAAGCCACCACATTTGCTGAGAAAGAAAACATATATTTCATGGAAACTTCTGCACTAGAGTCGTTGAATGTGGACAATGCTTTCGTGGAAGTGCTCACTCAGATATACAATGTTGTGAGTAAGAAGACTCTTGAGAAAGAGAATGGCTCTGCATCAGTGCCTAAGGGAGAGACTATCAATATTGGTAAAGATGATGTTTCAGATGTTAAGAAGAGTGGATGCTGCAGTACAGCATAG
Protein sequence

>MS.gene31413.t1

MGAVDEEYDYLFKLVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSVRILDKLVKAQIWDTAGQERYRAITSAYYRGAVGALLVYDSTRHVTFENVERWLKELRDHTDAYVVIMLVGNKADLRHLQAVSTEEATTFAEKENIYFMETSALESLNVDNAFVEVLTQIYNVVSKKTLEKENGSASVPKGETINIGKDDVSDVKKSGCCSTA