AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31441

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31441.t1 MTR_2g090620 94.949 396 18 1 1 396 1 394 0.0 734
MS.gene31441.t1 MTR_4g078740 61.511 278 101 1 109 386 35 306 1.33e-117 345
MS.gene31441.t1 MTR_2g460800 35.169 236 148 3 139 371 326 559 3.94e-46 167
MS.gene31441.t1 MTR_2g460800 36.129 155 94 3 139 290 326 478 4.82e-27 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31441.t1 AT2G20590 49.861 361 155 8 2 351 20 365 6.70e-104 313
MS.gene31441.t1 AT2G20590 49.315 365 164 8 2 351 20 378 5.42e-103 311
MS.gene31441.t1 AT4G28430 45.133 339 162 6 33 351 72 406 2.37e-93 288
MS.gene31441.t1 AT2G20590 50.162 309 133 8 2 295 20 322 1.60e-82 255
MS.gene31441.t1 AT5G58000 36.283 226 142 1 127 350 216 441 7.55e-47 167
MS.gene31441.t1 AT5G58000 37.383 214 132 1 139 350 334 547 1.99e-46 168

Find 74 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTCAAGGTTTCAGTTTC+AGG 0.086023 2.4:-10020385 MS.gene31441:intron
ACAATTGGCGATTCTCATTT+TGG 0.143148 2.4:-10019770 MS.gene31441:CDS
CTTCTACTAGGAGCTGAATT+TGG 0.262185 2.4:-10019379 MS.gene31441:CDS
ATCAAAACTCGAATCTTTAC+AGG 0.267508 2.4:-10018931 MS.gene31441:intron
GTTGGGTTGGTTGAAGAAAT+TGG 0.280135 2.4:-10020712 MS.gene31441:CDS
TAAATCAGATTCAGAAAATA+AGG 0.291934 2.4:-10018289 MS.gene31441:CDS
AACATTGTGGATTACTTTAA+GGG 0.298725 2.4:+10019490 None:intergenic
GTGTAGCAAGAATAAAGTTT+TGG 0.298854 2.4:+10019183 None:intergenic
CTTGAAGGTTGAGGAAAGTT+AGG 0.307696 2.4:+10020643 None:intergenic
TAGAATTTGAAGAAGAAAAT+GGG 0.333581 2.4:-10020520 MS.gene31441:CDS
ACAAAATCAACCCTTTGGTT+TGG 0.349573 2.4:-10020440 MS.gene31441:CDS
GTAGAATTTGAAGAAGAAAA+TGG 0.354309 2.4:-10020521 MS.gene31441:intron
GGGGTTGATTTAGATCATAT+TGG 0.354774 2.4:-10020500 MS.gene31441:CDS
ACTTCGTATCGCGGTTTCCT+TGG 0.378063 2.4:+10020766 None:intergenic
ACGGTCGATGAGACGGGTCT+TGG 0.378085 2.4:+10020876 None:intergenic
GAAAACAAAGATGGTGTTGT+TGG 0.391114 2.4:-10020730 MS.gene31441:CDS
GGGTTGATTTAGATCATATT+GGG 0.393926 2.4:-10020499 MS.gene31441:CDS
TCAACCCTTTGGTTTGGACT+TGG 0.396972 2.4:-10020434 MS.gene31441:CDS
TGTCTCGGCCTTGTCACAAT+TGG 0.407918 2.4:-10019785 MS.gene31441:CDS
TGGGCAGTTAGTGAGTGATT+TGG 0.427688 2.4:-10020480 MS.gene31441:CDS
ATTACTTTAAGGGTCATTGA+TGG 0.440294 2.4:+10019500 None:intergenic
TTGGCCACTTAATTACAATC+AGG 0.443528 2.4:-10019360 MS.gene31441:CDS
CACTCACAAGAAGAAATTAA+CGG 0.445955 2.4:-10018989 MS.gene31441:CDS
ATGTAACAAAATCAACCCTT+TGG 0.446627 2.4:-10020445 MS.gene31441:CDS
AGATTGTTGGATTGGTTTAA+CGG 0.447384 2.4:+10020948 None:intergenic
AGAGGGGAAGGGGAAGGTGA+AGG 0.450275 2.4:+10020907 None:intergenic
GGAAACTCAACTGGCATTAG+TGG 0.450988 2.4:-10018316 MS.gene31441:CDS
GGTGATGGAGATTGTTGGAT+TGG 0.451311 2.4:+10020940 None:intergenic
GGGTTTGAAACTATTGTTGT+TGG 0.463082 2.4:-10019028 MS.gene31441:CDS
TCAGCTCCTAGTAGAAGAAA+AGG 0.471363 2.4:+10019385 None:intergenic
GATTGGTTTAACGGTGATGA+TGG 0.471612 2.4:+10020957 None:intergenic
CAAAGATGGTGTTGTTGGGT+TGG 0.477270 2.4:-10020725 MS.gene31441:CDS
TTCAAGACGGTCGATGAGAC+GGG 0.478046 2.4:+10020870 None:intergenic
AACTATTGTTGTTGGATACA+TGG 0.478642 2.4:-10019020 MS.gene31441:CDS
AGGACCAGGGTGCTATTCTC+AGG 0.481892 2.4:-10019527 MS.gene31441:CDS
AGAAGAACAGGAAACTCAAC+TGG 0.484855 2.4:-10018325 MS.gene31441:CDS
GCCTCCTGATTGTAATTAAG+TGG 0.488567 2.4:+10019356 None:intergenic
GAAGGGGAAGGTGAAGGAGA+TGG 0.490751 2.4:+10020913 None:intergenic
ATGATGGGAAGTGATTGATT+TGG 0.501295 2.4:+10020973 None:intergenic
ATTAACATACTTGAAGGTTG+AGG 0.503849 2.4:+10020634 None:intergenic
TAGTGAGTGATTTGGTTATG+TGG 0.506027 2.4:-10020472 MS.gene31441:CDS
TTGATATTTCAGCATTGTCT+CGG 0.506205 2.4:-10019800 MS.gene31441:intron
GAAACTGCAAAATTTAAAGC+AGG 0.510333 2.4:+10019551 None:intergenic
AAAACAAAGATGGTGTTGTT+GGG 0.515013 2.4:-10020729 MS.gene31441:CDS
GTGATGGTGATGGAGATTGT+TGG 0.526109 2.4:+10020935 None:intergenic
GAAGGAGATGGTGATGGTGA+TGG 0.527359 2.4:+10020925 None:intergenic
ATCAGGAGGCTATGTGTCAC+TGG 0.536218 2.4:-10019343 MS.gene31441:intron
TCTCAAATTCAATTTCTCAA+AGG 0.543153 2.4:-10019735 MS.gene31441:intron
ATTCAACAAGAAAACAAAGA+TGG 0.548877 2.4:-10020739 MS.gene31441:CDS
TTTCAAGACGGTCGATGAGA+CGG 0.551751 2.4:+10020869 None:intergenic
TTTCTGAGAGGGGAAGGGGA+AGG 0.555742 2.4:+10020901 None:intergenic
ACATCCAAGTCCAAACCAAA+GGG 0.560703 2.4:+10020430 None:intergenic
TACATCCAAGTCCAAACCAA+AGG 0.566308 2.4:+10020429 None:intergenic
ATTCCAGAATGTCAAAAGTA+CGG 0.567233 2.4:+10018963 None:intergenic
GAAGGTGAAGGAGATGGTGA+TGG 0.569026 2.4:+10020919 None:intergenic
GCCACTTAATTACAATCAGG+AGG 0.572666 2.4:-10019357 MS.gene31441:CDS
ATATAACACTCGACCCAAGA+AGG 0.581043 2.4:-10020671 MS.gene31441:CDS
GGAAGTGATTGATTTGGTGA+TGG 0.584305 2.4:+10020979 None:intergenic
CAAGATGGAAAAGCACAAGT+AGG 0.610927 2.4:-10018390 MS.gene31441:CDS
AAAAGCACAAGTAGGTGAGA+AGG 0.616726 2.4:-10018382 MS.gene31441:CDS
AGAATTTGAAGAAGAAAATG+GGG 0.616889 2.4:-10020519 MS.gene31441:CDS
GATGAATCTCCTGAACCCGC+CGG 0.635645 2.4:-10020838 MS.gene31441:CDS
TGAATTTCAACTTCGTATCG+CGG 0.636174 2.4:+10020757 None:intergenic
CATCAGCGGCTGTTTCAAGA+CGG 0.637889 2.4:+10020857 None:intergenic
CAAATACTTAAAGCAGCATG+TGG 0.641110 2.4:-10018424 MS.gene31441:CDS
ATTGGTTTAACGGTGATGAT+GGG 0.641254 2.4:+10020958 None:intergenic
TTCTCCTGAGAATAGCACCC+TGG 0.662515 2.4:+10019523 None:intergenic
GAGAATCGCCAATTGTGACA+AGG 0.663769 2.4:+10019777 None:intergenic
CCCGCCGGTGCAAAACGTCG+TGG 0.664380 2.4:-10020823 MS.gene31441:CDS
GTGGTGCTGCAATTACAAGA+TGG 0.665984 2.4:-10018405 MS.gene31441:CDS
ACTAGTCAGATAGACAAGAG+AGG 0.678238 2.4:-10019162 MS.gene31441:intron
AAAACAACACTCGAAAACCA+AGG 0.680630 2.4:-10020783 MS.gene31441:CDS
CTATTTGTATTTCAGCAAAG+GGG 0.684853 2.4:-10019639 MS.gene31441:intron
GGGTTCAGGAGATTCATCAG+CGG 0.747685 2.4:+10020843 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAATAAAAAAAATT+AGG + chr2.4:10018720-10018739 None:intergenic 0.0%
!! AAAAAAAATAAAAAAAATTA+GGG + chr2.4:10018719-10018738 None:intergenic 0.0%
!! ATCTAGTCTATATAAAAAAA+TGG - chr2.4:10020609-10020628 MS.gene31441:intron 15.0%
!! AAAAGGTTATATAGATGATA+GGG + chr2.4:10019973-10019992 None:intergenic 20.0%
!! GTTTACTAAGCAAAATTAAA+GGG + chr2.4:10018937-10018956 None:intergenic 20.0%
!! TAAATCAGATTCAGAAAATA+AGG - chr2.4:10020986-10021005 MS.gene31441:CDS 20.0%
!! TAGAATTTGAAGAAGAAAAT+GGG - chr2.4:10018755-10018774 MS.gene31441:intron 20.0%
!! TATACATACTATAAACATAC+AGG - chr2.4:10020047-10020066 MS.gene31441:intron 20.0%
!!! ATTAATAATTTTCTTGTTGC+AGG - chr2.4:10019862-10019881 MS.gene31441:intron 20.0%
!!! CTTAGTAATTATTGATTAAG+TGG - chr2.4:10020444-10020463 MS.gene31441:CDS 20.0%
!!! TCTTATGATATCATTTTTGT+TGG - chr2.4:10020803-10020822 MS.gene31441:CDS 20.0%
! AATTCAGAATAAGAACATTG+TGG + chr2.4:10019801-10019820 None:intergenic 25.0%
! AATTGAACATAAATTTGTGC+AGG + chr2.4:10020511-10020530 None:intergenic 25.0%
! ACTATGAATCTTAAAATCCA+AGG - chr2.4:10020716-10020735 MS.gene31441:CDS 25.0%
! AGAATTTGAAGAAGAAAATG+GGG - chr2.4:10018756-10018775 MS.gene31441:intron 25.0%
! ATAAGAATCAACTTTGACAA+AGG + chr2.4:10020789-10020808 None:intergenic 25.0%
! ATTCAACAAGAAAACAAAGA+TGG - chr2.4:10018536-10018555 MS.gene31441:intron 25.0%
! ATTTGTGATCTTTATTCTCA+TGG - chr2.4:10019429-10019448 MS.gene31441:intron 25.0%
! CTTGAATGAACTATTGTTTA+CGG + chr2.4:10019259-10019278 None:intergenic 25.0%
! GAAAAGGTTATATAGATGAT+AGG + chr2.4:10019974-10019993 None:intergenic 25.0%
! GGTTTACTAAGCAAAATTAA+AGG + chr2.4:10018938-10018957 None:intergenic 25.0%
! GTAGAATTTGAAGAAGAAAA+TGG - chr2.4:10018754-10018773 MS.gene31441:intron 25.0%
! TAGTTAAGCAATCCAATTAA+AGG + chr2.4:10018959-10018978 None:intergenic 25.0%
! TCATGGTTATCATTGTAATA+TGG - chr2.4:10019203-10019222 MS.gene31441:CDS 25.0%
! TCCAAGGAGTTATTTATTTA+TGG - chr2.4:10020732-10020751 MS.gene31441:CDS 25.0%
! TCCATAAATAAATAACTCCT+TGG + chr2.4:10020736-10020755 None:intergenic 25.0%
! TCTATTTGTATTTCAGCAAA+GGG - chr2.4:10019635-10019654 MS.gene31441:intron 25.0%
! TCTCAAATTCAATTTCTCAA+AGG - chr2.4:10019540-10019559 MS.gene31441:CDS 25.0%
! TTCTATTTGTATTTCAGCAA+AGG - chr2.4:10019634-10019653 MS.gene31441:intron 25.0%
! TTGCTTAGTAAACCTTTAAT+TGG - chr2.4:10018944-10018963 MS.gene31441:CDS 25.0%
!! AACATTGTGGATTACTTTAA+GGG + chr2.4:10019788-10019807 None:intergenic 25.0%
!! CTTTAAATTTTGCAGTTTCA+AGG - chr2.4:10019728-10019747 MS.gene31441:intron 25.0%
!! GGACAGTGTTATTATATATA+TGG - chr2.4:10019224-10019243 MS.gene31441:intron 25.0%
!! GTGTTATATTTTCTCTTTCT+AGG + chr2.4:10018593-10018612 None:intergenic 25.0%
!! TGTAGCAAGAATAAAGTTTT+GGG + chr2.4:10020094-10020113 None:intergenic 25.0%
!!! TTGGATTTTAAGATTCATAG+TGG + chr2.4:10020717-10020736 None:intergenic 25.0%
AATCTCGGAATGATAGTTTA+GGG - chr2.4:10019114-10019133 MS.gene31441:intron 30.0%
AGCGAACTATAATTGACATT+TGG + chr2.4:10020006-10020025 None:intergenic 30.0%
ATCAAAACTCGAATCTTTAC+AGG - chr2.4:10020344-10020363 MS.gene31441:intron 30.0%
ATGTAACAAAATCAACCCTT+TGG - chr2.4:10018830-10018849 MS.gene31441:intron 30.0%
ATTAACATACTTGAAGGTTG+AGG + chr2.4:10018644-10018663 None:intergenic 30.0%
ATTAAGTGGAAAGATAACTG+TGG - chr2.4:10020458-10020477 MS.gene31441:CDS 30.0%
ATTCCAGAATGTCAAAAGTA+CGG + chr2.4:10020315-10020334 None:intergenic 30.0%
CACTCACAAGAAGAAATTAA+CGG - chr2.4:10020286-10020305 MS.gene31441:intron 30.0%
CATTGCATTAACATACTTGA+AGG + chr2.4:10018650-10018669 None:intergenic 30.0%
CTATTTGTATTTCAGCAAAG+GGG - chr2.4:10019636-10019655 MS.gene31441:intron 30.0%
GCGAACTATAATTGACATTT+GGG + chr2.4:10020005-10020024 None:intergenic 30.0%
TATATAGATGATAGGGTTCA+TGG + chr2.4:10019966-10019985 None:intergenic 30.0%
TTGATATTTCAGCATTGTCT+CGG - chr2.4:10019475-10019494 MS.gene31441:intron 30.0%
! AAAGTTAGGTTTTTCCTTCT+TGG + chr2.4:10018621-10018640 None:intergenic 30.0%
! AACTATTGTTGTTGGATACA+TGG - chr2.4:10020255-10020274 MS.gene31441:intron 30.0%
! AAGTTAGGTTTTTCCTTCTT+GGG + chr2.4:10018620-10018639 None:intergenic 30.0%
! ATTACTTTAAGGGTCATTGA+TGG + chr2.4:10019778-10019797 None:intergenic 30.0%
! GAAACTGCAAAATTTAAAGC+AGG + chr2.4:10019727-10019746 None:intergenic 30.0%
! GAACATTGTGGATTACTTTA+AGG + chr2.4:10019789-10019808 None:intergenic 30.0%
! GCTATTAGTTTCAATTGTTC+AGG - chr2.4:10019077-10019096 MS.gene31441:intron 30.0%
! GGGTTGATTTAGATCATATT+GGG - chr2.4:10018776-10018795 MS.gene31441:intron 30.0%
! GTAATTCCTTTTCTTCTACT+AGG - chr2.4:10019884-10019903 MS.gene31441:intron 30.0%
! GTGTAGCAAGAATAAAGTTT+TGG + chr2.4:10020095-10020114 None:intergenic 30.0%
! TTACTAAGACATTTTGCAGA+AGG + chr2.4:10020432-10020451 None:intergenic 30.0%
! TTATCTTCATGCTTTTCTCA+AGG - chr2.4:10018877-10018896 MS.gene31441:intron 30.0%
!! AAAACAAAGATGGTGTTGTT+GGG - chr2.4:10018546-10018565 MS.gene31441:intron 30.0%
!! AGATTGTTGGATTGGTTTAA+CGG + chr2.4:10018330-10018349 None:intergenic 30.0%
!! CATTTGGGTTTTCAAAGAAA+AGG + chr2.4:10019990-10020009 None:intergenic 30.0%
!!! GATTTTAAGATTCATAGTGG+CGG + chr2.4:10020714-10020733 None:intergenic 30.0%
!!! GATTTTTTGTTTACGCAGTT+TGG + chr2.4:10019316-10019335 None:intergenic 30.0%
!!! TAACTTGTTGAAGCTTTTGT+AGG - chr2.4:10020159-10020178 MS.gene31441:intron 30.0%
AAAACAACACTCGAAAACCA+AGG - chr2.4:10018492-10018511 MS.gene31441:intron 35.0%
ACAAAATCAACCCTTTGGTT+TGG - chr2.4:10018835-10018854 MS.gene31441:intron 35.0%
ATCGGAGAGAGTGATAATTT+AGG - chr2.4:10019008-10019027 MS.gene31441:CDS 35.0%
CAAATACTTAAAGCAGCATG+TGG - chr2.4:10020851-10020870 MS.gene31441:CDS 35.0%
GAATCTCGGAATGATAGTTT+AGG - chr2.4:10019113-10019132 MS.gene31441:intron 35.0%
GAGTTTATTCAGCAATATGC+TGG - chr2.4:10020652-10020671 MS.gene31441:CDS 35.0%
GGAACAACAGAAAAAAGAAC+CGG - chr2.4:10020914-10020933 MS.gene31441:CDS 35.0%
TAAACTCCAATACATTGCAG+TGG + chr2.4:10020639-10020658 None:intergenic 35.0%
TCAATTGTTCAGGATCAGAT+TGG - chr2.4:10019087-10019106 MS.gene31441:intron 35.0%
TCTATTTAGCCCCTCAAAAT+CGG - chr2.4:10018990-10019009 MS.gene31441:CDS 35.0%
TGAATTTCAACTTCGTATCG+CGG + chr2.4:10018521-10018540 None:intergenic 35.0%
TTATATGCATATGCAGCTGA+GGG - chr2.4:10020227-10020246 MS.gene31441:intron 35.0%
TTCTCATGGAGAAAAAACTG+AGG - chr2.4:10019443-10019462 MS.gene31441:intron 35.0%
TTGGCCACTTAATTACAATC+AGG - chr2.4:10019915-10019934 MS.gene31441:intron 35.0%
TTTCTCAAGGTTTCAGTTTC+AGG - chr2.4:10018890-10018909 MS.gene31441:intron 35.0%
! ACAATTGGCGATTCTCATTT+TGG - chr2.4:10019505-10019524 MS.gene31441:CDS 35.0%
! ATGATGGGAAGTGATTGATT+TGG + chr2.4:10018305-10018324 None:intergenic 35.0%
! ATTGGTTTAACGGTGATGAT+GGG + chr2.4:10018320-10018339 None:intergenic 35.0%
! CAATTGGCGATTCTCATTTT+GGG - chr2.4:10019506-10019525 MS.gene31441:CDS 35.0%
! GGGGTTGATTTAGATCATAT+TGG - chr2.4:10018775-10018794 MS.gene31441:intron 35.0%
! GGGTTTGAAACTATTGTTGT+TGG - chr2.4:10020247-10020266 MS.gene31441:intron 35.0%
! TCAATTTTTCTCTGTGTGCT+AGG - chr2.4:10020525-10020544 MS.gene31441:intron 35.0%
!! GAAAACAAAGATGGTGTTGT+TGG - chr2.4:10018545-10018564 MS.gene31441:intron 35.0%
!! TAGTGAGTGATTTGGTTATG+TGG - chr2.4:10018803-10018822 MS.gene31441:intron 35.0%
AAGCACAAAAGATGGGCAAA+GGG - chr2.4:10019168-10019187 MS.gene31441:CDS 40.0%
ACAACAGAAAAAAGAACCGG+TGG - chr2.4:10020917-10020936 MS.gene31441:CDS 40.0%
ACATCCAAGTCCAAACCAAA+GGG + chr2.4:10018848-10018867 None:intergenic 40.0%
ACTAGTCAGATAGACAAGAG+AGG - chr2.4:10020113-10020132 MS.gene31441:intron 40.0%
AGAAGAACAGGAAACTCAAC+TGG - chr2.4:10020950-10020969 MS.gene31441:CDS 40.0%
ATATAACACTCGACCCAAGA+AGG - chr2.4:10018604-10018623 MS.gene31441:intron 40.0%
CTTCTACTAGGAGCTGAATT+TGG - chr2.4:10019896-10019915 MS.gene31441:intron 40.0%
CTTGAAGGTTGAGGAAAGTT+AGG + chr2.4:10018635-10018654 None:intergenic 40.0%
GACCCATAAGCACAAAAGAT+GGG - chr2.4:10019161-10019180 MS.gene31441:intron 40.0%
GATCAGATTGGAGAGAATCT+CGG - chr2.4:10019099-10019118 MS.gene31441:intron 40.0%
GCCACTTAATTACAATCAGG+AGG - chr2.4:10019918-10019937 MS.gene31441:intron 40.0%
GCCTCCTGATTGTAATTAAG+TGG + chr2.4:10019922-10019941 None:intergenic 40.0%
GGTGGTAAAAACAGAAGAAC+AGG - chr2.4:10020938-10020957 MS.gene31441:CDS 40.0%
GTTATATGCATATGCAGCTG+AGG - chr2.4:10020226-10020245 MS.gene31441:intron 40.0%
TAAGCACAAAAGATGGGCAA+AGG - chr2.4:10019167-10019186 MS.gene31441:CDS 40.0%
TACATCCAAGTCCAAACCAA+AGG + chr2.4:10018849-10018868 None:intergenic 40.0%
TCAGCTCCTAGTAGAAGAAA+AGG + chr2.4:10019893-10019912 None:intergenic 40.0%
TGACCCATAAGCACAAAAGA+TGG - chr2.4:10019160-10019179 MS.gene31441:intron 40.0%
! AAAAGCACAAGTAGGTGAGA+AGG - chr2.4:10020893-10020912 MS.gene31441:CDS 40.0%
! ATCACTCTCTCCGATTTTGA+GGG + chr2.4:10019003-10019022 None:intergenic 40.0%
! ATTTTGCAGTTTCAAGGACC+AGG - chr2.4:10019734-10019753 MS.gene31441:intron 40.0%
! ATTTTTCTCTGTGTGCTAGG+TGG - chr2.4:10020528-10020547 MS.gene31441:intron 40.0%
! CAAGATGGAAAAGCACAAGT+AGG - chr2.4:10020885-10020904 MS.gene31441:CDS 40.0%
! TATCACTCTCTCCGATTTTG+AGG + chr2.4:10019004-10019023 None:intergenic 40.0%
! TGCCCATCTTTTGTGCTTAT+GGG + chr2.4:10019166-10019185 None:intergenic 40.0%
! TGTCCGTACTTTTGACATTC+TGG - chr2.4:10020309-10020328 MS.gene31441:intron 40.0%
! TTGCCCATCTTTTGTGCTTA+TGG + chr2.4:10019167-10019186 None:intergenic 40.0%
! TTTTGCAGTTTCAAGGACCA+GGG - chr2.4:10019735-10019754 MS.gene31441:intron 40.0%
!! GATTGGTTTAACGGTGATGA+TGG + chr2.4:10018321-10018340 None:intergenic 40.0%
!! GGAAGTGATTGATTTGGTGA+TGG + chr2.4:10018299-10018318 None:intergenic 40.0%
!! GTTGGGTTGGTTGAAGAAAT+TGG - chr2.4:10018563-10018582 MS.gene31441:intron 40.0%
!! TGTGCAGGAGTGATTTGTTA+AGG + chr2.4:10020496-10020515 None:intergenic 40.0%
!!! AGTGTTGTTTTGAGTGAGTC+TGG + chr2.4:10018483-10018502 None:intergenic 40.0%
!!! TCTTCTGTTTTTACCACCAC+CGG + chr2.4:10020936-10020955 None:intergenic 40.0%
AAAAGATGGGCAAAGGGCTT+TGG - chr2.4:10019174-10019193 MS.gene31441:CDS 45.0%
AACTACAGTTGCTGCACACT+TGG - chr2.4:10019290-10019309 MS.gene31441:intron 45.0%
ACAGAAAAAAGAACCGGTGG+TGG - chr2.4:10020920-10020939 MS.gene31441:CDS 45.0%
CTCCAATACATTGCAGTGGA+AGG + chr2.4:10020635-10020654 None:intergenic 45.0%
GAGAATCGCCAATTGTGACA+AGG + chr2.4:10019501-10019520 None:intergenic 45.0%
GGAAACTCAACTGGCATTAG+TGG - chr2.4:10020959-10020978 MS.gene31441:CDS 45.0%
TGGGCAGTTAGTGAGTGATT+TGG - chr2.4:10018795-10018814 MS.gene31441:intron 45.0%
TTTCAAGACGGTCGATGAGA+CGG + chr2.4:10018409-10018428 None:intergenic 45.0%
! AAGGGCTTTGGTACAGTTCA+TGG - chr2.4:10019186-10019205 MS.gene31441:CDS 45.0%
! GTTGGCAGCTTTTATGTTGC+TGG - chr2.4:10020821-10020840 MS.gene31441:CDS 45.0%
! TCACTCTCTCCGATTTTGAG+GGG + chr2.4:10019002-10019021 None:intergenic 45.0%
!! CAAAGATGGTGTTGTTGGGT+TGG - chr2.4:10018550-10018569 MS.gene31441:intron 45.0%
!! GGGTCTTGGATTTTCTGAGA+GGG + chr2.4:10018388-10018407 None:intergenic 45.0%
!! GGTCTTGGATTTTCTGAGAG+GGG + chr2.4:10018387-10018406 None:intergenic 45.0%
!! GGTGATGGAGATTGTTGGAT+TGG + chr2.4:10018338-10018357 None:intergenic 45.0%
!! GTGATGGTGATGGAGATTGT+TGG + chr2.4:10018343-10018362 None:intergenic 45.0%
!! GTGGTGCTGCAATTACAAGA+TGG - chr2.4:10020870-10020889 MS.gene31441:CDS 45.0%
!! TCAACCCTTTGGTTTGGACT+TGG - chr2.4:10018841-10018860 MS.gene31441:intron 45.0%
!! TGGATTTTCTGAGAGGGGAA+GGG + chr2.4:10018382-10018401 None:intergenic 45.0%
!! TTGGATTTTCTGAGAGGGGA+AGG + chr2.4:10018383-10018402 None:intergenic 45.0%
ACTTCGTATCGCGGTTTCCT+TGG + chr2.4:10018512-10018531 None:intergenic 50.0%
ATCAGGAGGCTATGTGTCAC+TGG - chr2.4:10019932-10019951 MS.gene31441:intron 50.0%
GAAGGTGAAGGAGATGGTGA+TGG + chr2.4:10018359-10018378 None:intergenic 50.0%
GGCCTTCCACTGCAATGTAT+TGG - chr2.4:10020630-10020649 MS.gene31441:intron 50.0%
GGGTTCAGGAGATTCATCAG+CGG + chr2.4:10018435-10018454 None:intergenic 50.0%
TTCAAGACGGTCGATGAGAC+GGG + chr2.4:10018408-10018427 None:intergenic 50.0%
TTCTCCTGAGAATAGCACCC+TGG + chr2.4:10019755-10019774 None:intergenic 50.0%
! CATCAGCGGCTGTTTCAAGA+CGG + chr2.4:10018421-10018440 None:intergenic 50.0%
! CGGGTCTTGGATTTTCTGAG+AGG + chr2.4:10018389-10018408 None:intergenic 50.0%
! GAGGCTATGTGTCACTGGTT+AGG - chr2.4:10019937-10019956 MS.gene31441:intron 50.0%
! TGTCTCGGCCTTGTCACAAT+TGG - chr2.4:10019490-10019509 MS.gene31441:intron 50.0%
! TTTGCCACGACGTTTTGCAC+CGG + chr2.4:10018459-10018478 None:intergenic 50.0%
!! GAAGGAGATGGTGATGGTGA+TGG + chr2.4:10018353-10018372 None:intergenic 50.0%
!! GGATTTTCTGAGAGGGGAAG+GGG + chr2.4:10018381-10018400 None:intergenic 50.0%
GAAGGGGAAGGTGAAGGAGA+TGG + chr2.4:10018365-10018384 None:intergenic 55.0%
GATGAATCTCCTGAACCCGC+CGG - chr2.4:10018437-10018456 MS.gene31441:CDS 55.0%
! TTTCTGAGAGGGGAAGGGGA+AGG + chr2.4:10018377-10018396 None:intergenic 55.0%
!! AGGACCAGGGTGCTATTCTC+AGG - chr2.4:10019748-10019767 MS.gene31441:CDS 55.0%
ACGGTCGATGAGACGGGTCT+TGG + chr2.4:10018402-10018421 None:intergenic 60.0%
AGAGGGGAAGGGGAAGGTGA+AGG + chr2.4:10018371-10018390 None:intergenic 60.0%
! GCCACGACGTTTTGCACCGG+CGG + chr2.4:10018456-10018475 None:intergenic 65.0%
!! CCACGACGTTTTGCACCGGC+GGG + chr2.4:10018455-10018474 None:intergenic 65.0%
!! CGTTTTGCACCGGCGGGTTC+AGG + chr2.4:10018449-10018468 None:intergenic 65.0%
CCCGCCGGTGCAAAACGTCG+TGG - chr2.4:10018452-10018471 MS.gene31441:intron 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 10018281 10021016 10018281 ID=MS.gene31441
chr2.4 mRNA 10018281 10021016 10018281 ID=MS.gene31441.t1;Parent=MS.gene31441
chr2.4 exon 10020644 10021016 10020644 ID=MS.gene31441.t1.exon1;Parent=MS.gene31441.t1
chr2.4 CDS 10020644 10021016 10020644 ID=cds.MS.gene31441.t1;Parent=MS.gene31441.t1
chr2.4 exon 10020386 10020539 10020386 ID=MS.gene31441.t1.exon2;Parent=MS.gene31441.t1
chr2.4 CDS 10020386 10020539 10020386 ID=cds.MS.gene31441.t1;Parent=MS.gene31441.t1
chr2.4 exon 10019736 10019810 10019736 ID=MS.gene31441.t1.exon3;Parent=MS.gene31441.t1
chr2.4 CDS 10019736 10019810 10019736 ID=cds.MS.gene31441.t1;Parent=MS.gene31441.t1
chr2.4 exon 10019505 10019646 10019505 ID=MS.gene31441.t1.exon4;Parent=MS.gene31441.t1
chr2.4 CDS 10019505 10019646 10019505 ID=cds.MS.gene31441.t1;Parent=MS.gene31441.t1
chr2.4 exon 10019344 10019413 10019344 ID=MS.gene31441.t1.exon5;Parent=MS.gene31441.t1
chr2.4 CDS 10019344 10019413 10019344 ID=cds.MS.gene31441.t1;Parent=MS.gene31441.t1
chr2.4 exon 10019163 10019228 10019163 ID=MS.gene31441.t1.exon6;Parent=MS.gene31441.t1
chr2.4 CDS 10019163 10019228 10019163 ID=cds.MS.gene31441.t1;Parent=MS.gene31441.t1
chr2.4 exon 10018932 10019054 10018932 ID=MS.gene31441.t1.exon7;Parent=MS.gene31441.t1
chr2.4 CDS 10018932 10019054 10018932 ID=cds.MS.gene31441.t1;Parent=MS.gene31441.t1
chr2.4 exon 10018281 10018468 10018281 ID=MS.gene31441.t1.exon8;Parent=MS.gene31441.t1
chr2.4 CDS 10018281 10018468 10018281 ID=cds.MS.gene31441.t1;Parent=MS.gene31441.t1
Gene Sequence

>MS.gene31441

ATGGATTCATCAACACCATCACCAAATCAATCACTTCCCATCATCACCGTTAAACCAATCCAACAATCTCCATCACCATCACCATCTCCTTCACCTTCCCCTTCCCCTCTCAGAAAATCCAAGACCCGTCTCATCGACCGTCTTGAAACAGCCGCTGATGAATCTCCTGAACCCGCCGGTGCAAAACGTCGTGGCAAAACCAGACTCACTCAAAACAACACTCGAAAACCAAGGAAACCGCGATACGAAGTTGAAATTCAACAAGAAAACAAAGATGGTGTTGTTGGGTTGGTTGAAGAAATTGGAAAACCTAGAAAGAGAAAATATAACACTCGACCCAAGAAGGAAAAACCTAACTTTCCTCAACCTTCAAGTATGTTAATGCAATGTATACTTCATATTTGAAATTATCTAACTGTTTTTGTTTCAATGAATCCCTAATTTTTTTTATTTTTTTTTTTGTGATTTTGTGTGTAGAATTTGAAGAAGAAAATGGGGTTGATTTAGATCATATTGGGCAGTTAGTGAGTGATTTGGTTATGTGGAAAGATGTAACAAAATCAACCCTTTGGTTTGGACTTGGATGTATTTGTCTCTTATCTTCATGCTTTTCTCAAGGTTTCAGTTTCAGGTATTGCAATTTTTTCTTATAACCCTTTAATTTTGCTTAGTAAACCTTTAATTGGATTGCTTAACTATTACTTTTTCTTCTATTTAGCCCCTCAAAATCGGAGAGAGTGATAATTTAGGATTGATTTGATAGATTGAGAACAGTTTAGTTTTAGAGATTATTTATGCTATTAGTTTCAATTGTTCAGGATCAGATTGGAGAGAATCTCGGAATGATAGTTTAGGGACGAAATTGAAAGTTGTTTAGTATGACCCATAAGCACAAAAGATGGGCAAAGGGCTTTGGTACAGTTCATGGTTATCATTGTAATATGGACAGTGTTATTATATATATGGCTTGTAACACCGTAAACAATAGTTCATTCAAGTAGTAGCAAGAAACTACAGTTGCTGCACACTTGGCCAAACTGCGTAAACAAAAAATCGTGTTCATTGTCTTGTGATTGCTCGTTTCTTTGATCTTCAATTACAAGTTGCTGTTCTAACAAAAGTTAGTTTATTTTTTATTTTTTTTTTAAATTTGTGATCTTTATTCTCATGGAGAAAAAACTGAGGTTTTCATACTTGATATTTCAGCATTGTCTCGGCCTTGTCACAATTGGCGATTCTCATTTTGGGTGTTTCATTTTTCTCAAATTCAATTTCTCAAAGGTACTCTTTTGCTTCTGATAGCTAATTATTCTCTAAACTATCTATTTCATTTAGTGTTGTTATTTTATGATTTTCTATTTGTATTTCAGCAAAGGGGTTGAAGAGAAGAGTTATGTCAAGATTAAAGAAGATGATGTTTTGCGTCTTGCAAAATTGATTCTTCCTGCTTTAAATTTTGCAGTTTCAAGGACCAGGGTGCTATTCTCAGGAGAACCATCAATGACCCTTAAAGTAATCCACAATGTTCTTATTCTGAATTTGATTTTGTATAATCAGATCTATAATAATTGCTAACAAATGATTAATAATTTTCTTGTTGCAGGTAATTCCTTTTCTTCTACTAGGAGCTGAATTTGGCCACTTAATTACAATCAGGAGGCTATGTGTCACTGGTTAGGACACCATGAACCCTATCATCTATATAACCTTTTCTTTGAAAACCCAAATGTCAATTATAGTTCGCTTTTTGTCATTGTTAAAATGCTTATACATACTATAAACATACAGGTTTTTTTGTCAGCTTCACAATCCCAAAACTTTATTCTTGCTACACTAGTCAGATAGACAAGAGAGGTACGTATGACTTCATGATCACACTAACTTGTTGAAGCTTTTGTAGGTATCTATATATACACTTTTTTGTGCTTAATAGTTGAAAGCTTGTGTTATATGCATATGCAGCTGAGGGTTTGAAACTATTGTTGTTGGATACATGGTGTGCTTGCACTCACAAGAAGAAATTAACGGTGTCCGTACTTTTGACATTCTGGAATCTATCTTCAATCAAAACTCGAATCTTTACAGGTAACACTTGCATAAGTAATTATTATAGCATAATAAGTTCAGATCATTTATACTAATTCACAGCCTTCTGCAAAATGTCTTAGTAATTATTGATTAAGTGGAAAGATAACTGTGGTTTGTTCTGTCACCTTAACAAATCACTCCTGCACAAATTTATGTTCAATTTTTCTCTGTGTGCTAGGTGGATACAATTTCTTACATGTTTTGCTGTTGTTTTATCACTACACAAAAATTTAATCTTCTATCTAGTCTATATAAAAAAATGGCCTTCCACTGCAATGTATTGGAGTTTATTCAGCAATATGCTGGTTCTTTTAGACTCATGTTGCAACACATGAGTTACTGCCGCCACTATGAATCTTAAAATCCAAGGAGTTATTTATTTATGGACAGATATATTTGCATATATTGATTTTAAAACCTTTGTCAAAGTTGATTCTTATGATATCATTTTTGTTGGCAGCTTTTATGTTGCTGGTACTATTCAAATACTTAAAGCAGCATGTGGTGCTGCAATTACAAGATGGAAAAGCACAAGTAGGTGAGAAGGAACAACAGAAAAAAGAACCGGTGGTGGTAAAAACAGAAGAACAGGAAACTCAACTGGCATTAGTGGTACTTAAATCAGATTCAGAAAATAAGGAGAATTAA
Protein sequence

>MS.gene31441.t1

MDSSTPSPNQSLPIITVKPIQQSPSPSPSPSPSPSPLRKSKTRLIDRLETAADESPEPAGAKRRGKTRLTQNNTRKPRKPRYEVEIQQENKDGVVGLVEEIGKPRKRKYNTRPKKEKPNFPQPSKFEEENGVDLDHIGQLVSDLVMWKDVTKSTLWFGLGCICLLSSCFSQGFSFSIVSALSQLAILILGVSFFSNSISQSKGVEEKSYVKIKEDDVLRLAKLILPALNFAVSRTRVLFSGEPSMTLKVIPFLLLGAEFGHLITIRRLCVTGFFVSFTIPKLYSCYTSQIDKRAEGLKLLLLDTWCACTHKKKLTVSVLLTFWNLSSIKTRIFTAFMLLVLFKYLKQHVVLQLQDGKAQVGEKEQQKKEPVVVKTEEQETQLALVVLKSDSENKEN