Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31717.t1 | PNX85949.1 | 84.9 | 311 | 46 | 1 | 1 | 310 | 60 | 370 | 4.60E-157 | 563.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31717.t1 | Q6AZB8 | 25.2 | 258 | 154 | 10 | 19 | 260 | 81 | 315 | 1.1e-10 | 68.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31717.t1 | A0A2K3M5A0 | 84.9 | 311 | 46 | 1 | 1 | 310 | 60 | 370 | 3.3e-157 | 563.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052973 | MS.gene31717 | 0.845159 | 4.83E-59 | -1.69E-46 |
| MS.gene057576 | MS.gene31717 | 0.807095 | 5.98E-50 | -1.69E-46 |
| MS.gene057584 | MS.gene31717 | 0.809852 | 1.54E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31717.t1 | MTR_1g085720 | 77.143 | 70 | 16 | 0 | 1 | 70 | 34 | 103 | 1.95e-34 | 122 |
| MS.gene31717.t1 | MTR_4g055725 | 33.529 | 170 | 92 | 4 | 3 | 172 | 110 | 258 | 3.37e-21 | 91.3 |
| MS.gene31717.t1 | MTR_4g117120 | 33.962 | 106 | 64 | 2 | 1 | 106 | 183 | 282 | 1.91e-13 | 70.5 |
| MS.gene31717.t1 | MTR_8g073195 | 37.624 | 101 | 60 | 2 | 209 | 309 | 47 | 144 | 1.08e-12 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31717.t1 | AT1G43722 | 42.986 | 221 | 119 | 3 | 5 | 219 | 100 | 319 | 5.08e-59 | 192 |
| MS.gene31717.t1 | AT5G41980 | 33.074 | 257 | 154 | 4 | 7 | 263 | 78 | 316 | 5.43e-46 | 160 |
| MS.gene31717.t1 | AT3G30525 | 39.623 | 159 | 90 | 2 | 84 | 237 | 1 | 158 | 2.39e-35 | 126 |
| MS.gene31717.t1 | AT5G28730 | 34.807 | 181 | 92 | 3 | 5 | 184 | 58 | 213 | 5.39e-34 | 126 |
| MS.gene31717.t1 | AT5G35695 | 37.956 | 137 | 72 | 2 | 121 | 256 | 24 | 148 | 3.66e-26 | 103 |
| MS.gene31717.t1 | AT5G28950 | 41.758 | 91 | 53 | 0 | 63 | 153 | 8 | 98 | 3.58e-22 | 90.9 |
| MS.gene31717.t1 | AT4G10890 | 42.857 | 91 | 51 | 1 | 133 | 223 | 71 | 160 | 4.41e-18 | 85.1 |
| MS.gene31717.t1 | AT4G10890 | 42.857 | 91 | 51 | 1 | 133 | 223 | 71 | 160 | 4.41e-18 | 85.1 |
| MS.gene31717.t1 | AT4G10890 | 42.857 | 91 | 51 | 1 | 133 | 223 | 71 | 160 | 4.41e-18 | 85.1 |
Find 52 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCTATCATCCTTGATTT+TGG | 0.117739 | 1.1:-12810661 | None:intergenic |
| AGAAATGTTCAAAATAAATT+TGG | 0.175729 | 1.1:+12810528 | MS.gene31717:CDS |
| TTCTTTCAATGACATTTCTA+AGG | 0.228551 | 1.1:-12811194 | None:intergenic |
| CAAAATAAATTTGGAAAATC+AGG | 0.272245 | 1.1:+12810537 | MS.gene31717:CDS |
| AAAGATCAAATTCGTTATAT+TGG | 0.275852 | 1.1:+12810753 | MS.gene31717:CDS |
| GATAGATATCCTTTCATGTT+TGG | 0.305736 | 1.1:-12811078 | None:intergenic |
| AGGCACTTGTGATGTTTCTT+TGG | 0.313453 | 1.1:+12810481 | MS.gene31717:CDS |
| CGAGCATATATCCTTGGATT+TGG | 0.329097 | 1.1:-12811368 | None:intergenic |
| CCGATGCATCCTTCAAAATA+AGG | 0.333834 | 1.1:-12810690 | None:intergenic |
| TTTGTTGTTGTTGGCTGGCC+TGG | 0.350368 | 1.1:+12810837 | MS.gene31717:CDS |
| TCTTGTTTGATTAGTGGAAA+TGG | 0.370259 | 1.1:-12811264 | None:intergenic |
| TTGAAAGAACAATTGGTGTT+TGG | 0.373420 | 1.1:+12811208 | MS.gene31717:CDS |
| TAACGAATTTGATCTTTAGT+AGG | 0.379665 | 1.1:-12810747 | None:intergenic |
| ATTCGTTATATTGGCAGAAA+AGG | 0.394672 | 1.1:+12810762 | MS.gene31717:CDS |
| TCAAGGATGATAGAAGATAT+TGG | 0.401243 | 1.1:+12810667 | MS.gene31717:CDS |
| CTCGTTCGAGCATATATCCT+TGG | 0.409241 | 1.1:-12811374 | None:intergenic |
| TCAAATAGCCACTAAGTTGT+TGG | 0.422025 | 1.1:+12811479 | MS.gene31717:CDS |
| AATGTCATTGAAAGAACAAT+TGG | 0.424218 | 1.1:+12811201 | MS.gene31717:CDS |
| GGATGCATCGGTGCTATAGA+TGG | 0.428232 | 1.1:+12810702 | MS.gene31717:CDS |
| AAGATGAGGAATTCAACAAA+TGG | 0.440534 | 1.1:+12811343 | MS.gene31717:CDS |
| TTCACTAAACTTCCTACTTA+CGG | 0.442099 | 1.1:-12810565 | None:intergenic |
| TGACTGCCATCTCTAAAATC+AGG | 0.452335 | 1.1:-12811129 | None:intergenic |
| CAATTGGTGTTTGGAAGAAA+AGG | 0.453010 | 1.1:+12811217 | MS.gene31717:CDS |
| TGATATCTTTCACCTTTGTA+TGG | 0.453127 | 1.1:-12811102 | None:intergenic |
| ATTTCTGTGAAGTTAAAATC+AGG | 0.454308 | 1.1:-12810633 | None:intergenic |
| AAGTATTACTTGGTTGATGC+AGG | 0.461050 | 1.1:+12811051 | MS.gene31717:CDS |
| CATAACTTCATTCGAATATG+TGG | 0.472793 | 1.1:+12811315 | MS.gene31717:CDS |
| CGTGTGTCATGCGCAGTACC+AGG | 0.480441 | 1.1:-12810855 | None:intergenic |
| ATTTCTAGGAAAGTATTACT+TGG | 0.498326 | 1.1:+12811041 | MS.gene31717:intron |
| AGAAATTCCATCCAAAATCA+AGG | 0.500971 | 1.1:+12810650 | MS.gene31717:CDS |
| ATCAAACGAAATGACATCCT+TGG | 0.510213 | 1.1:+12810458 | None:intergenic |
| ATCGTACCTTCAGGAGGATG+AGG | 0.518203 | 1.1:-12810918 | None:intergenic |
| AGATGAGGAATTCAACAAAT+GGG | 0.524003 | 1.1:+12811344 | MS.gene31717:CDS |
| TCTTTCAATGACATTTCTAA+GGG | 0.531707 | 1.1:-12811193 | None:intergenic |
| TATAATTTAAGAGTACATGA+TGG | 0.540136 | 1.1:+12811432 | MS.gene31717:CDS |
| AAACATCACAAGTGCCTCCA+AGG | 0.555823 | 1.1:-12810475 | None:intergenic |
| AGTACATGATGGGAGCTACA+TGG | 0.581291 | 1.1:+12811443 | MS.gene31717:CDS |
| AACAAATGGGACCAAATCCA+AGG | 0.586140 | 1.1:+12811357 | MS.gene31717:CDS |
| ATAATTTAAGAGTACATGAT+GGG | 0.587184 | 1.1:+12811433 | MS.gene31717:CDS |
| GTGTTCCCTCATCCTCCTGA+AGG | 0.587543 | 1.1:+12810912 | MS.gene31717:CDS |
| AAACGAAATGACATCCTTGG+AGG | 0.589096 | 1.1:+12810461 | None:intergenic |
| GCAGGATATCCAAACATGAA+AGG | 0.592144 | 1.1:+12811069 | MS.gene31717:CDS |
| TACTAGCAAAAGAAATTGTG+AGG | 0.592380 | 1.1:+12810607 | MS.gene31717:CDS |
| TTGGTGTTTGGAAGAAAAGG+TGG | 0.597185 | 1.1:+12811220 | MS.gene31717:CDS |
| AATCAGGAGAGACCGTAAGT+AGG | 0.608714 | 1.1:+12810553 | MS.gene31717:CDS |
| AATATGTGGCGTTCAAGATG+AGG | 0.609133 | 1.1:+12811329 | MS.gene31717:CDS |
| TCTGTGAAGTTAAAATCAGG+AGG | 0.623384 | 1.1:-12810630 | None:intergenic |
| TCGTACCTTCAGGAGGATGA+GGG | 0.629341 | 1.1:-12810917 | None:intergenic |
| TTGATCTTTAGTAGGAACGA+TGG | 0.638791 | 1.1:-12810739 | None:intergenic |
| AGAGATGGCAGTCAAGCACA+AGG | 0.648134 | 1.1:+12811138 | MS.gene31717:CDS |
| AAATTAATCGTACCTTCAGG+AGG | 0.657477 | 1.1:-12810924 | None:intergenic |
| GGATATCTATCACCATACAA+AGG | 0.686019 | 1.1:+12811090 | MS.gene31717:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAATGTTCAAAATAAATT+TGG | + | chr1.1:12810528-12810547 | MS.gene31717:CDS | 15.0% |
| !! | ATGATTTATATAGTATTACT+TGG | + | chr1.1:12810968-12810987 | MS.gene31717:intron | 15.0% |
| !! | AAAGATCAAATTCGTTATAT+TGG | + | chr1.1:12810753-12810772 | MS.gene31717:CDS | 20.0% |
| !! | CAAAATAAATTTGGAAAATC+AGG | + | chr1.1:12810537-12810556 | MS.gene31717:CDS | 20.0% |
| !!! | ATAATTTAAGAGTACATGAT+GGG | + | chr1.1:12811433-12811452 | MS.gene31717:CDS | 20.0% |
| !!! | TATAATTTAAGAGTACATGA+TGG | + | chr1.1:12811432-12811451 | MS.gene31717:CDS | 20.0% |
| ! | AAAAAATTAATCGTACCTTC+AGG | - | chr1.1:12810930-12810949 | None:intergenic | 25.0% |
| ! | AATGTCATTGAAAGAACAAT+TGG | + | chr1.1:12811201-12811220 | MS.gene31717:CDS | 25.0% |
| ! | ATTTCTGTGAAGTTAAAATC+AGG | - | chr1.1:12810636-12810655 | None:intergenic | 25.0% |
| ! | TAACGAATTTGATCTTTAGT+AGG | - | chr1.1:12810750-12810769 | None:intergenic | 25.0% |
| ! | TCTTTCAATGACATTTCTAA+GGG | - | chr1.1:12811196-12811215 | None:intergenic | 25.0% |
| ! | TTCTTTCAATGACATTTCTA+AGG | - | chr1.1:12811197-12811216 | None:intergenic | 25.0% |
| !! | ACACATTTTTCATTTCTTCA+AGG | - | chr1.1:12810896-12810915 | None:intergenic | 25.0% |
| !!! | ATCTTTTCTTGTTTGATTAG+TGG | - | chr1.1:12811273-12811292 | None:intergenic | 25.0% |
| !!! | ATTTCTAGGAAAGTATTACT+TGG | + | chr1.1:12811041-12811060 | MS.gene31717:intron | 25.0% |
| !!! | TTAATCACTTTTGTTGTTGT+TGG | + | chr1.1:12810828-12810847 | MS.gene31717:CDS | 25.0% |
| AAGATGAGGAATTCAACAAA+TGG | + | chr1.1:12811343-12811362 | MS.gene31717:CDS | 30.0% | |
| AGAAATTCCATCCAAAATCA+AGG | + | chr1.1:12810650-12810669 | MS.gene31717:CDS | 30.0% | |
| AGATGAGGAATTCAACAAAT+GGG | + | chr1.1:12811344-12811363 | MS.gene31717:CDS | 30.0% | |
| ATTCGTTATATTGGCAGAAA+AGG | + | chr1.1:12810762-12810781 | MS.gene31717:CDS | 30.0% | |
| CATAACTTCATTCGAATATG+TGG | + | chr1.1:12811315-12811334 | MS.gene31717:CDS | 30.0% | |
| GATAGATATCCTTTCATGTT+TGG | - | chr1.1:12811081-12811100 | None:intergenic | 30.0% | |
| TACTAGCAAAAGAAATTGTG+AGG | + | chr1.1:12810607-12810626 | MS.gene31717:CDS | 30.0% | |
| TCAAGGATGATAGAAGATAT+TGG | + | chr1.1:12810667-12810686 | MS.gene31717:CDS | 30.0% | |
| TGATATCTTTCACCTTTGTA+TGG | - | chr1.1:12811105-12811124 | None:intergenic | 30.0% | |
| TTCACTAAACTTCCTACTTA+CGG | - | chr1.1:12810568-12810587 | None:intergenic | 30.0% | |
| ! | TCTTCTATCATCCTTGATTT+TGG | - | chr1.1:12810664-12810683 | None:intergenic | 30.0% |
| ! | TCTTGTTTGATTAGTGGAAA+TGG | - | chr1.1:12811267-12811286 | None:intergenic | 30.0% |
| ! | TTGAAAGAACAATTGGTGTT+TGG | + | chr1.1:12811208-12811227 | MS.gene31717:CDS | 30.0% |
| !! | TTTGTTTTCCAACAACTTAG+TGG | - | chr1.1:12811490-12811509 | None:intergenic | 30.0% |
| !!! | AGATATTGGCCTTATTTTGA+AGG | + | chr1.1:12810681-12810700 | MS.gene31717:CDS | 30.0% |
| AAATTAATCGTACCTTCAGG+AGG | - | chr1.1:12810927-12810946 | None:intergenic | 35.0% | |
| GGATATCTATCACCATACAA+AGG | + | chr1.1:12811090-12811109 | MS.gene31717:CDS | 35.0% | |
| TCAAATAGCCACTAAGTTGT+TGG | + | chr1.1:12811479-12811498 | MS.gene31717:CDS | 35.0% | |
| TCTGTGAAGTTAAAATCAGG+AGG | - | chr1.1:12810633-12810652 | None:intergenic | 35.0% | |
| TTGATCTTTAGTAGGAACGA+TGG | - | chr1.1:12810742-12810761 | None:intergenic | 35.0% | |
| ! | AAGTATTACTTGGTTGATGC+AGG | + | chr1.1:12811051-12811070 | MS.gene31717:CDS | 35.0% |
| ! | ACTAACATGACATTTTGCGT+TGG | - | chr1.1:12810792-12810811 | None:intergenic | 35.0% |
| ! | CAATTGGTGTTTGGAAGAAA+AGG | + | chr1.1:12811217-12811236 | MS.gene31717:CDS | 35.0% |
| ! | CTATCATCCTTGATTTTGGA+TGG | - | chr1.1:12810660-12810679 | None:intergenic | 35.0% |
| !! | CACATTCCTGATTTTAGAGA+TGG | + | chr1.1:12811123-12811142 | MS.gene31717:CDS | 35.0% |
| !!! | CCTTATTTTGAAGGATGCAT+CGG | + | chr1.1:12810690-12810709 | MS.gene31717:CDS | 35.0% |
| AACAAATGGGACCAAATCCA+AGG | + | chr1.1:12811357-12811376 | MS.gene31717:CDS | 40.0% | |
| AATATGTGGCGTTCAAGATG+AGG | + | chr1.1:12811329-12811348 | MS.gene31717:CDS | 40.0% | |
| AGGCACTTGTGATGTTTCTT+TGG | + | chr1.1:12810481-12810500 | MS.gene31717:CDS | 40.0% | |
| CCGATGCATCCTTCAAAATA+AGG | - | chr1.1:12810693-12810712 | None:intergenic | 40.0% | |
| CGAGCATATATCCTTGGATT+TGG | - | chr1.1:12811371-12811390 | None:intergenic | 40.0% | |
| GCAGGATATCCAAACATGAA+AGG | + | chr1.1:12811069-12811088 | MS.gene31717:CDS | 40.0% | |
| ! | TTGGTGTTTGGAAGAAAAGG+TGG | + | chr1.1:12811220-12811239 | MS.gene31717:CDS | 40.0% |
| !! | TGACTGCCATCTCTAAAATC+AGG | - | chr1.1:12811132-12811151 | None:intergenic | 40.0% |
| !!! | TCACTTTTGTTGTTGTTGGC+TGG | + | chr1.1:12810832-12810851 | MS.gene31717:CDS | 40.0% |
| AAACATCACAAGTGCCTCCA+AGG | - | chr1.1:12810478-12810497 | None:intergenic | 45.0% | |
| AATCAGGAGAGACCGTAAGT+AGG | + | chr1.1:12810553-12810572 | MS.gene31717:CDS | 45.0% | |
| CTCGTTCGAGCATATATCCT+TGG | - | chr1.1:12811377-12811396 | None:intergenic | 45.0% | |
| ! | AGTACATGATGGGAGCTACA+TGG | + | chr1.1:12811443-12811462 | MS.gene31717:CDS | 45.0% |
| !!! | TTTTATTTTTATATATTTCT+AGG | + | chr1.1:12811027-12811046 | MS.gene31717:intron | 5.0% |
| AGAGATGGCAGTCAAGCACA+AGG | + | chr1.1:12811138-12811157 | MS.gene31717:CDS | 50.0% | |
| ATCGTACCTTCAGGAGGATG+AGG | - | chr1.1:12810921-12810940 | None:intergenic | 50.0% | |
| TCGTACCTTCAGGAGGATGA+GGG | - | chr1.1:12810920-12810939 | None:intergenic | 50.0% | |
| !! | GGATGCATCGGTGCTATAGA+TGG | + | chr1.1:12810702-12810721 | MS.gene31717:CDS | 50.0% |
| !! | TTTGTTGTTGTTGGCTGGCC+TGG | + | chr1.1:12810837-12810856 | MS.gene31717:CDS | 50.0% |
| GTGTTCCCTCATCCTCCTGA+AGG | + | chr1.1:12810912-12810931 | MS.gene31717:CDS | 55.0% | |
| CGTGTGTCATGCGCAGTACC+AGG | - | chr1.1:12810858-12810877 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 12810468 | 12811527 | 12810468 | ID=MS.gene31717 |
| chr1.1 | mRNA | 12810468 | 12811527 | 12810468 | ID=MS.gene31717.t1;Parent=MS.gene31717 |
| chr1.1 | exon | 12810468 | 12810933 | 12810468 | ID=MS.gene31717.t1.exon1;Parent=MS.gene31717.t1 |
| chr1.1 | CDS | 12810468 | 12810933 | 12810468 | ID=cds.MS.gene31717.t1;Parent=MS.gene31717.t1 |
| chr1.1 | exon | 12811049 | 12811527 | 12811049 | ID=MS.gene31717.t1.exon2;Parent=MS.gene31717.t1 |
| chr1.1 | CDS | 12811049 | 12811527 | 12811049 | ID=cds.MS.gene31717.t1;Parent=MS.gene31717.t1 |
| Gene Sequence |
| Protein sequence |
