Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057576.t1 | XP_003621282.2 | 73 | 215 | 17 | 4 | 24 | 217 | 23 | 217 | 1.20E-34 | 156.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057576.t1 | G7L237 | 73.0 | 215 | 17 | 4 | 24 | 217 | 23 | 217 | 8.4e-35 | 156.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057576 | MS.gene057577 | 0.906558 | 1.31E-80 | -1.69E-46 |
| MS.gene057576 | MS.gene057580 | 0.846201 | 2.52E-59 | -1.69E-46 |
| MS.gene057576 | MS.gene057581 | 0.838542 | 2.70E-57 | -1.69E-46 |
| MS.gene057576 | MS.gene057584 | 0.948355 | 1.11E-106 | -1.69E-46 |
| MS.gene057576 | MS.gene057586 | 0.91803 | 2.57E-86 | -1.69E-46 |
| MS.gene057576 | MS.gene057588 | 0.939536 | 1.08E-99 | -1.69E-46 |
| MS.gene057576 | MS.gene057591 | 0.871641 | 5.86E-67 | -1.69E-46 |
| MS.gene057576 | MS.gene058074 | 0.820077 | 8.30E-53 | -1.69E-46 |
| MS.gene057576 | MS.gene06279 | 0.852457 | 4.56E-61 | -1.69E-46 |
| MS.gene057576 | MS.gene063783 | 0.812564 | 3.98E-51 | -1.69E-46 |
| MS.gene057576 | MS.gene067233 | 0.803339 | 3.65E-49 | -1.69E-46 |
| MS.gene057576 | MS.gene07959 | 0.804602 | 2.00E-49 | -1.69E-46 |
| MS.gene057576 | MS.gene08108 | 0.849367 | 3.38E-60 | -1.69E-46 |
| MS.gene057576 | MS.gene29383 | 0.836832 | 7.42E-57 | -1.69E-46 |
| MS.gene057576 | MS.gene31717 | 0.807095 | 5.98E-50 | -1.69E-46 |
| MS.gene057576 | MS.gene33763 | 0.804565 | 2.03E-49 | -1.69E-46 |
| MS.gene057576 | MS.gene33875 | 0.804806 | 1.81E-49 | -1.69E-46 |
| MS.gene057576 | MS.gene42767 | 0.801334 | 9.46E-49 | -1.69E-46 |
| MS.gene057576 | MS.gene44240 | 0.807549 | 4.79E-50 | -1.69E-46 |
| MS.gene057576 | MS.gene45357 | 0.88212 | 1.36E-70 | -1.69E-46 |
| MS.gene057576 | MS.gene49515 | 0.892183 | 2.01E-74 | -1.69E-46 |
| MS.gene057576 | MS.gene49520 | 0.905942 | 2.52E-80 | -1.69E-46 |
| MS.gene057576 | MS.gene50109 | 0.825972 | 3.50E-54 | -1.69E-46 |
| MS.gene057576 | MS.gene61644 | 0.803914 | 2.78E-49 | -1.69E-46 |
| MS.gene057576 | MS.gene62423 | 0.807037 | 6.15E-50 | -1.69E-46 |
| MS.gene057576 | MS.gene66979 | 0.816443 | 5.52E-52 | -1.69E-46 |
| MS.gene057576 | MS.gene73012 | 0.811958 | 5.39E-51 | -1.69E-46 |
| MS.gene057576 | MS.gene74985 | 0.805086 | 1.58E-49 | -1.69E-46 |
| MS.gene057576 | MS.gene86038 | 0.818763 | 1.65E-52 | -1.69E-46 |
| MS.gene057576 | MS.gene86061 | 0.812259 | 4.64E-51 | -1.69E-46 |
| MS.gene057576 | MS.gene87969 | 0.813199 | 2.89E-51 | -1.69E-46 |
| MS.gene057576 | MS.gene92223 | 0.84503 | 5.24E-59 | -1.69E-46 |
| MS.gene057576 | MS.gene95728 | 0.815488 | 9.01E-52 | -1.69E-46 |
| MS.gene057576 | MS.gene99915 | 0.811125 | 8.18E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057576.t1 | MTR_7g011390 | 75.630 | 238 | 16 | 5 | 1 | 217 | 1 | 217 | 5.09e-80 | 238 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGGCTGCTGCTGGCATCCA+AGG | 0.270786 | 7.2:+85859871 | None:intergenic |
| ATCCAACGGCTGGCTGCTGC+TGG | 0.310560 | 7.2:+85859862 | None:intergenic |
| CAGTTTCTTCTTCTTAGACT+TGG | 0.357409 | 7.2:+85861206 | None:intergenic |
| GTGCACATGCCAACACAATA+TGG | 0.384828 | 7.2:-85860285 | MS.gene057576:CDS |
| TGAGGATAAAGCTGCTGTTC+AGG | 0.399727 | 7.2:-85859807 | MS.gene057576:CDS |
| TGGCTGCTGCTGGCATCCAA+GGG | 0.406161 | 7.2:+85859872 | None:intergenic |
| GTGAAGCTGTATCATCTTCA+TGG | 0.410616 | 7.2:+85860319 | None:intergenic |
| GGTCCAATATCTAGAGTAGT+TGG | 0.437672 | 7.2:+85859774 | None:intergenic |
| TTATGATCAGATTTATCGTC+AGG | 0.438391 | 7.2:-85861070 | MS.gene057576:intron |
| ACCTCCTTGCCATATTGTGT+TGG | 0.455217 | 7.2:+85860276 | None:intergenic |
| GGCATGTGCACAGGTTGAAT+TGG | 0.460863 | 7.2:+85860297 | None:intergenic |
| TGCCAGCAGCAGCCAGCCGT+TGG | 0.479830 | 7.2:-85859864 | MS.gene057576:CDS |
| TATTGTGTTGGCATGTGCAC+AGG | 0.498215 | 7.2:+85860288 | None:intergenic |
| GCAGGCCCGCAAAGCAAAAG+AGG | 0.498850 | 7.2:-85860743 | MS.gene057576:CDS |
| TGAAAATGATATGAAGCCCT+TGG | 0.509252 | 7.2:-85859888 | MS.gene057576:CDS |
| GTCCAATATCTAGAGTAGTT+GGG | 0.512418 | 7.2:+85859775 | None:intergenic |
| TCGTTCGTTCCCATGGGTGG+AGG | 0.527930 | 7.2:-85861255 | None:intergenic |
| AGCCTCTTCTGCTAATCGAC+GGG | 0.530366 | 7.2:+85860837 | None:intergenic |
| TGTTGAGCTCATCCAACGGC+TGG | 0.538954 | 7.2:+85859852 | None:intergenic |
| AACTCGTTCGTTCCCATGGG+TGG | 0.547111 | 7.2:-85861258 | None:intergenic |
| AGCCCGTCGATTAGCAAAAG+AGG | 0.550604 | 7.2:-85860785 | MS.gene057576:CDS |
| AGCCCGTCGATTAGCAGAAG+AGG | 0.554670 | 7.2:-85860839 | MS.gene057576:CDS |
| GATAAAGCTGCTGTTCAGGA+GGG | 0.586121 | 7.2:-85859803 | MS.gene057576:CDS |
| CTCTTGCTCTAAATTCACTC+TGG | 0.587930 | 7.2:-85860708 | MS.gene057576:CDS |
| GAGCCTCTTCTGCTAATCGA+CGG | 0.589148 | 7.2:+85860836 | None:intergenic |
| GACTTGTTGAGCTCATCCAA+CGG | 0.593483 | 7.2:+85859848 | None:intergenic |
| ATAAAGCTGCTGTTCAGGAG+GGG | 0.607327 | 7.2:-85859802 | MS.gene057576:CDS |
| GGATAAAGCTGCTGTTCAGG+AGG | 0.611925 | 7.2:-85859804 | MS.gene057576:CDS |
| GAACAGCAGCTTTATCCTCA+AGG | 0.621167 | 7.2:+85859810 | None:intergenic |
| AACACAAAACGTTAGCCTTG+AGG | 0.622562 | 7.2:-85859825 | MS.gene057576:CDS |
| CATGCCAACACAATATGGCA+AGG | 0.661413 | 7.2:-85860280 | MS.gene057576:CDS |
| GCCAACACAATATGGCAAGG+AGG | 0.737684 | 7.2:-85860277 | MS.gene057576:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACTAAATGTTATGAAAAT+TGG | + | chr7.2:85859874-85859893 | None:intergenic | 15.0% |
| !! | TTATAAGAAATTAAGCAAAA+GGG | - | chr7.2:85861076-85861095 | MS.gene057576:CDS | 15.0% |
| !!! | ATTTTTAATTATGTATTTGC+AGG | - | chr7.2:85861109-85861128 | MS.gene057576:intron | 15.0% |
| !! | AATTTCTTATAAGTAAGGAA+AGG | + | chr7.2:85861068-85861087 | None:intergenic | 20.0% |
| !! | ATGTTCTGTTATTGTTAATT+TGG | - | chr7.2:85860015-85860034 | MS.gene057576:intron | 20.0% |
| !! | CTTATAAGAAATTAAGCAAA+AGG | - | chr7.2:85861075-85861094 | MS.gene057576:CDS | 20.0% |
| !! | TGCTTAATTTCTTATAAGTA+AGG | + | chr7.2:85861073-85861092 | None:intergenic | 20.0% |
| !!! | AATAAAAAAAGTCTTGTAGA+AGG | + | chr7.2:85860780-85860799 | None:intergenic | 20.0% |
| !!! | CATAGCTTTTCTTTAAAATT+GGG | - | chr7.2:85860976-85860995 | MS.gene057576:intron | 20.0% |
| !!! | TGTTATTGTTAATTTGGTTA+TGG | - | chr7.2:85860021-85860040 | MS.gene057576:intron | 20.0% |
| !!! | TTTTAACTAACTTGTTATGT+TGG | - | chr7.2:85859989-85860008 | MS.gene057576:intron | 20.0% |
| ! | AAAATTGGAATGATAGATAG+AGG | + | chr7.2:85859859-85859878 | None:intergenic | 25.0% |
| ! | AAATTGGAATGATAGATAGA+GGG | + | chr7.2:85859858-85859877 | None:intergenic | 25.0% |
| ! | TAAAAAAAAAGCAGAAGAAG+AGG | - | chr7.2:85860218-85860237 | MS.gene057576:intron | 25.0% |
| !! | GTTTTCTTAAAAATTCCTCT+AGG | - | chr7.2:85860600-85860619 | MS.gene057576:intron | 25.0% |
| !! | GTTTTCTTAAAAATTGCTCT+AGG | - | chr7.2:85860436-85860455 | MS.gene057576:intron | 25.0% |
| !!! | ACTTGGATTTCTTTTTTGTT+CGG | + | chr7.2:85859807-85859826 | None:intergenic | 25.0% |
| !!! | CCAATTTTAAAGAAAAGCTA+TGG | + | chr7.2:85860978-85860997 | None:intergenic | 25.0% |
| !!! | CCATAGCTTTTCTTTAAAAT+TGG | - | chr7.2:85860975-85860994 | MS.gene057576:intron | 25.0% |
| !!! | GCAATTTTTAAGAAAACCTA+CGG | + | chr7.2:85860433-85860452 | None:intergenic | 25.0% |
| !!! | GGAATTTTTAAGAAAACCTA+CGG | + | chr7.2:85860597-85860616 | None:intergenic | 25.0% |
| AGATGAAAAGTGAATAGAAG+TGG | + | chr7.2:85860910-85860929 | None:intergenic | 30.0% | |
| GAATAGAAGTGGTATACTTA+TGG | + | chr7.2:85860899-85860918 | None:intergenic | 30.0% | |
| TTATGATCAGATTTATCGTC+AGG | - | chr7.2:85859957-85859976 | MS.gene057576:intron | 30.0% | |
| AATATCCAGACACACACATT+AGG | + | chr7.2:85860845-85860864 | None:intergenic | 35.0% | |
| CAAAAAAGTAGCAGAAGAAG+AGG | - | chr7.2:85860164-85860183 | MS.gene057576:intron | 35.0% | |
| CAGTTTCTTCTTCTTAGACT+TGG | + | chr7.2:85859824-85859843 | None:intergenic | 35.0% | |
| CTTCTTGCTAATACACCAAA+TGG | - | chr7.2:85861002-85861021 | MS.gene057576:intron | 35.0% | |
| GAAAGTAAGAGAGACAGAAA+CGG | + | chr7.2:85860096-85860115 | None:intergenic | 35.0% | |
| GATAGTACCTACAAATACGA+TGG | + | chr7.2:85860132-85860151 | None:intergenic | 35.0% | |
| TCATCTTCCATCGTATTTGT+AGG | - | chr7.2:85860122-85860141 | MS.gene057576:intron | 35.0% | |
| TCTTGCTCAAAAAAAACTGC+AGG | - | chr7.2:85860266-85860285 | MS.gene057576:intron | 35.0% | |
| TGAAAATGATATGAAGCCCT+TGG | - | chr7.2:85861139-85861158 | MS.gene057576:intron | 35.0% | |
| ! | AGCGTTTTTGTAGTCATGTT+TGG | + | chr7.2:85860378-85860397 | None:intergenic | 35.0% |
| ! | ATGGATTGACATGAATGTTC+AGG | + | chr7.2:85860880-85860899 | None:intergenic | 35.0% |
| ! | CATGATGATGATGATGATCT+TGG | - | chr7.2:85860060-85860079 | MS.gene057576:intron | 35.0% |
| ! | GAAAAAGCGTATCATCTTCA+TGG | + | chr7.2:85860547-85860566 | None:intergenic | 35.0% |
| ! | GCGTTTTTGTAGTCATGTTT+GGG | + | chr7.2:85860377-85860396 | None:intergenic | 35.0% |
| ! | TTTTGTAATCTGTATGCAGC+TGG | - | chr7.2:85860520-85860539 | MS.gene057576:intron | 35.0% |
| ! | TTTTGTAGTCATGTTTGGGT+AGG | + | chr7.2:85860373-85860392 | None:intergenic | 35.0% |
| !! | AAGCACGTTGTTTCTTAGTA+AGG | + | chr7.2:85860477-85860496 | None:intergenic | 35.0% |
| !! | CGTTGTTTCTTAGTAAGGAA+AGG | + | chr7.2:85860472-85860491 | None:intergenic | 35.0% |
| !!! | CTCTTCTTCTGCTACTTTTT+TGG | + | chr7.2:85860166-85860185 | None:intergenic | 35.0% |
| AAAGCTACAAACAAAGCCGT+AGG | - | chr7.2:85860578-85860597 | MS.gene057576:intron | 40.0% | |
| AAAGGACAAGACAATCCTAG+AGG | + | chr7.2:85860454-85860473 | None:intergenic | 40.0% | |
| AAATCTACAAACACAGCCGT+AGG | - | chr7.2:85860414-85860433 | MS.gene057576:intron | 40.0% | |
| AACAGGAGATAGGTTTATGC+AGG | + | chr7.2:85860812-85860831 | None:intergenic | 40.0% | |
| CAGTTTCATGAACAGGAGAT+AGG | + | chr7.2:85860822-85860841 | None:intergenic | 40.0% | |
| CATTAGGCAGTTTCATGAAC+AGG | + | chr7.2:85860829-85860848 | None:intergenic | 40.0% | |
| GATAAGAAGACAGTCCTAGA+GGG | + | chr7.2:85861046-85861065 | None:intergenic | 40.0% | |
| GTGAAGCTGTATCATCTTCA+TGG | + | chr7.2:85860711-85860730 | None:intergenic | 40.0% | |
| TTGGAATGATAGATAGAGGG+AGG | + | chr7.2:85859855-85859874 | None:intergenic | 40.0% | |
| ! | CTCTTGCTCTAAATTCACTC+TGG | - | chr7.2:85860319-85860338 | MS.gene057576:CDS | 40.0% |
| !! | AACACAAAACGTTAGCCTTG+AGG | - | chr7.2:85861202-85861221 | MS.gene057576:intron | 40.0% |
| AACTGCCTAATGTGTGTGTC+TGG | - | chr7.2:85860837-85860856 | MS.gene057576:CDS | 45.0% | |
| ACCTCCTTGCCATATTGTGT+TGG | + | chr7.2:85860754-85860773 | None:intergenic | 45.0% | |
| ATAAAGCTGCTGTTCAGGAG+GGG | - | chr7.2:85861225-85861244 | MS.gene057576:CDS | 45.0% | |
| CATGCCAACACAATATGGCA+AGG | - | chr7.2:85860747-85860766 | MS.gene057576:CDS | 45.0% | |
| GATAAAGCTGCTGTTCAGGA+GGG | - | chr7.2:85861224-85861243 | MS.gene057576:CDS | 45.0% | |
| GGATAAGAAGACAGTCCTAG+AGG | + | chr7.2:85861047-85861066 | None:intergenic | 45.0% | |
| GTGCACATGCCAACACAATA+TGG | - | chr7.2:85860742-85860761 | MS.gene057576:CDS | 45.0% | |
| TGAGGATAAAGCTGCTGTTC+AGG | - | chr7.2:85861220-85861239 | MS.gene057576:CDS | 45.0% | |
| ! | AGCCTCTTTTGCTAATCGAC+GGG | + | chr7.2:85860247-85860266 | None:intergenic | 45.0% |
| ! | GAACAGCAGCTTTATCCTCA+AGG | + | chr7.2:85861220-85861239 | None:intergenic | 45.0% |
| ! | GAGCCTCTTTTGCTAATCGA+CGG | + | chr7.2:85860248-85860267 | None:intergenic | 45.0% |
| ! | TATTGTGTTGGCATGTGCAC+AGG | + | chr7.2:85860742-85860761 | None:intergenic | 45.0% |
| !! | GACTTGTTGAGCTCATCCAA+CGG | + | chr7.2:85861182-85861201 | None:intergenic | 45.0% |
| !! | TACAAAATAAAAATAAAAAA+TGG | + | chr7.2:85859914-85859933 | None:intergenic | 5.0% |
| AGCCTCTTCTGCTAATCGAC+GGG | + | chr7.2:85860193-85860212 | None:intergenic | 50.0% | |
| CACCAAATGGAGAGAGCCTT+AGG | - | chr7.2:85861015-85861034 | MS.gene057576:intron | 50.0% | |
| GAGCCTCTTCTGCTAATCGA+CGG | + | chr7.2:85860194-85860213 | None:intergenic | 50.0% | |
| GCCAACACAATATGGCAAGG+AGG | - | chr7.2:85860750-85860769 | MS.gene057576:CDS | 50.0% | |
| GGATAAAGCTGCTGTTCAGG+AGG | - | chr7.2:85861223-85861242 | MS.gene057576:CDS | 50.0% | |
| TGCCTAAGGCTCTCTCCATT+TGG | + | chr7.2:85861020-85861039 | None:intergenic | 50.0% | |
| ! | AGCCCGTCGATTAGCAAAAG+AGG | - | chr7.2:85860242-85860261 | MS.gene057576:intron | 50.0% |
| ! | CTGCTGCCTCTTTTGCTTTG+CGG | + | chr7.2:85860293-85860312 | None:intergenic | 50.0% |
| ! | GGCATGTGCACAGGTTGAAT+TGG | + | chr7.2:85860733-85860752 | None:intergenic | 50.0% |
| ! | TGCTGCCTCTTTTGCTTTGC+GGG | + | chr7.2:85860292-85860311 | None:intergenic | 50.0% |
| AGCCTTAGGCAATGCCCTCT+AGG | - | chr7.2:85861029-85861048 | MS.gene057576:intron | 55.0% | |
| TGTTGAGCTCATCCAACGGC+TGG | + | chr7.2:85861178-85861197 | None:intergenic | 55.0% | |
| ! | AGCCCGTCGATTAGCAGAAG+AGG | - | chr7.2:85860188-85860207 | MS.gene057576:intron | 55.0% |
| !! | GTCCTAGAGGGCATTGCCTA+AGG | + | chr7.2:85861034-85861053 | None:intergenic | 55.0% |
| GCAGGCCCGCAAAGCAAAAG+AGG | - | chr7.2:85860284-85860303 | MS.gene057576:CDS | 60.0% | |
| TGGCTGCTGCTGGCATCCAA+GGG | + | chr7.2:85861158-85861177 | None:intergenic | 60.0% | |
| CTGGCTGCTGCTGGCATCCA+AGG | + | chr7.2:85861159-85861178 | None:intergenic | 65.0% | |
| ! | ATCCAACGGCTGGCTGCTGC+TGG | + | chr7.2:85861168-85861187 | None:intergenic | 65.0% |
| TGCCAGCAGCAGCCAGCCGT+TGG | - | chr7.2:85861163-85861182 | MS.gene057576:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 85859784 | 85861265 | 85859784 | ID=MS.gene057576 |
| chr7.2 | mRNA | 85859784 | 85861265 | 85859784 | ID=MS.gene057576.t1;Parent=MS.gene057576 |
| chr7.2 | exon | 85861205 | 85861265 | 85861205 | ID=MS.gene057576.t1.exon1;Parent=MS.gene057576.t1 |
| chr7.2 | CDS | 85861205 | 85861265 | 85861205 | ID=cds.MS.gene057576.t1;Parent=MS.gene057576.t1 |
| chr7.2 | exon | 85861071 | 85861114 | 85861071 | ID=MS.gene057576.t1.exon2;Parent=MS.gene057576.t1 |
| chr7.2 | CDS | 85861071 | 85861114 | 85861071 | ID=cds.MS.gene057576.t1;Parent=MS.gene057576.t1 |
| chr7.2 | exon | 85860618 | 85860905 | 85860618 | ID=MS.gene057576.t1.exon3;Parent=MS.gene057576.t1 |
| chr7.2 | CDS | 85860618 | 85860905 | 85860618 | ID=cds.MS.gene057576.t1;Parent=MS.gene057576.t1 |
| chr7.2 | exon | 85860454 | 85860510 | 85860454 | ID=MS.gene057576.t1.exon4;Parent=MS.gene057576.t1 |
| chr7.2 | CDS | 85860454 | 85860510 | 85860454 | ID=cds.MS.gene057576.t1;Parent=MS.gene057576.t1 |
| chr7.2 | exon | 85860278 | 85860346 | 85860278 | ID=MS.gene057576.t1.exon5;Parent=MS.gene057576.t1 |
| chr7.2 | CDS | 85860278 | 85860346 | 85860278 | ID=cds.MS.gene057576.t1;Parent=MS.gene057576.t1 |
| chr7.2 | exon | 85859784 | 85859918 | 85859784 | ID=MS.gene057576.t1.exon6;Parent=MS.gene057576.t1 |
| chr7.2 | CDS | 85859784 | 85859918 | 85859784 | ID=cds.MS.gene057576.t1;Parent=MS.gene057576.t1 |
| Gene Sequence |
| Protein sequence |
