Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31907.t1 | XP_024641575.1 | 87.8 | 131 | 14 | 1 | 1 | 131 | 317 | 445 | 1.10E-40 | 176 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31907.t1 | A0A396JLF3 | 87.8 | 131 | 14 | 1 | 1 | 131 | 317 | 445 | 8.1e-41 | 176.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055486 | MS.gene31907 | 0.808742 | 2.67E-50 | -1.69E-46 |
| MS.gene056448 | MS.gene31907 | 0.810003 | 1.43E-50 | -1.69E-46 |
| MS.gene060837 | MS.gene31907 | 0.81277 | 3.59E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATAGGGCTTGCGCTATTT+TGG | 0.253203 | 1.4:-19722284 | None:intergenic |
| TCTGCAGCAACAAAGGTTAA+TGG | 0.357357 | 1.4:+19722429 | MS.gene31907:CDS |
| GCAGAGAATAATGGAAATAT+TGG | 0.362372 | 1.4:+19722558 | MS.gene31907:CDS |
| TCTGAAAATTTCAACAATGA+AGG | 0.390896 | 1.4:+19722213 | MS.gene31907:CDS |
| AACTTGGAAGCAGAGAATAA+TGG | 0.409292 | 1.4:+19722549 | MS.gene31907:CDS |
| TCTTCAATACTAAGAAGCTT+TGG | 0.417560 | 1.4:+19722393 | MS.gene31907:CDS |
| TATTGAAGAAGAAGTATTGT+TGG | 0.444187 | 1.4:-19722379 | None:intergenic |
| CTGAAAATTTCAACAATGAA+GGG | 0.449011 | 1.4:+19722214 | MS.gene31907:CDS |
| GCTGACATGTGAGGAGAGAT+AGG | 0.463590 | 1.4:-19722300 | None:intergenic |
| AGAAGTATTGTTGGTGGTGG+TGG | 0.473552 | 1.4:-19722370 | None:intergenic |
| AATGGAAATATTGGTGGTGG+AGG | 0.493890 | 1.4:+19722567 | MS.gene31907:CDS |
| AATAATGGAAATATTGGTGG+TGG | 0.516109 | 1.4:+19722564 | MS.gene31907:CDS |
| AGAAACATGAATGGAGGAGT+TGG | 0.532530 | 1.4:+19722504 | MS.gene31907:CDS |
| GAGAATAATGGAAATATTGG+TGG | 0.544890 | 1.4:+19722561 | MS.gene31907:CDS |
| AAGGTTAATGGTGGTGGTGA+TGG | 0.546808 | 1.4:+19722441 | MS.gene31907:CDS |
| ATTAACCTTTGTTGCTGCAG+AGG | 0.549390 | 1.4:-19722427 | None:intergenic |
| TTCTTCCTCTGCAGCAACAA+AGG | 0.557640 | 1.4:+19722422 | MS.gene31907:CDS |
| ATAGTTATGAGAAACATGAA+TGG | 0.565754 | 1.4:+19722495 | MS.gene31907:CDS |
| ATGGAAATATTGGTGGTGGA+GGG | 0.575851 | 1.4:+19722568 | MS.gene31907:CDS |
| TGAAGAAGAAGTATTGTTGG+TGG | 0.581655 | 1.4:-19722376 | None:intergenic |
| GTTATGAGAAACATGAATGG+AGG | 0.584520 | 1.4:+19722498 | MS.gene31907:CDS |
| AAGTCAACGTAATTTCAACT+TGG | 0.588729 | 1.4:+19722533 | MS.gene31907:CDS |
| GCAACAAAGGTTAATGGTGG+TGG | 0.590964 | 1.4:+19722435 | MS.gene31907:CDS |
| AGAAGAAGTATTGTTGGTGG+TGG | 0.609899 | 1.4:-19722373 | None:intergenic |
| CTGACATGTGAGGAGAGATA+GGG | 0.611438 | 1.4:-19722299 | None:intergenic |
| GCAGCAACAAAGGTTAATGG+TGG | 0.676561 | 1.4:+19722432 | MS.gene31907:CDS |
| AAAGCTGTTGCTGACATGTG+AGG | 0.698667 | 1.4:-19722309 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATAGTTATGAGAAACATGAA+TGG | + | chr1.4:19722495-19722514 | MS.gene31907:CDS | 25.0% |
| ! | CTGAAAATTTCAACAATGAA+GGG | + | chr1.4:19722214-19722233 | MS.gene31907:CDS | 25.0% |
| ! | TCTGAAAATTTCAACAATGA+AGG | + | chr1.4:19722213-19722232 | MS.gene31907:CDS | 25.0% |
| !!! | TATTGAAGAAGAAGTATTGT+TGG | - | chr1.4:19722382-19722401 | None:intergenic | 25.0% |
| AAGTCAACGTAATTTCAACT+TGG | + | chr1.4:19722533-19722552 | MS.gene31907:CDS | 30.0% | |
| CTTCAAAAAGCTTCTCAAAT+GGG | + | chr1.4:19722333-19722352 | MS.gene31907:CDS | 30.0% | |
| TCTTCAATACTAAGAAGCTT+TGG | + | chr1.4:19722393-19722412 | MS.gene31907:CDS | 30.0% | |
| ! | AATAATGGAAATATTGGTGG+TGG | + | chr1.4:19722564-19722583 | MS.gene31907:CDS | 30.0% |
| ! | GAGAATAATGGAAATATTGG+TGG | + | chr1.4:19722561-19722580 | MS.gene31907:CDS | 30.0% |
| ! | GCAGAGAATAATGGAAATAT+TGG | + | chr1.4:19722558-19722577 | MS.gene31907:CDS | 30.0% |
| AACTTGGAAGCAGAGAATAA+TGG | + | chr1.4:19722549-19722568 | MS.gene31907:CDS | 35.0% | |
| GCTTCAAAAAGCTTCTCAAA+TGG | + | chr1.4:19722332-19722351 | MS.gene31907:CDS | 35.0% | |
| GTTATGAGAAACATGAATGG+AGG | + | chr1.4:19722498-19722517 | MS.gene31907:CDS | 35.0% | |
| ! | ACTAGAGATTTTCTTGGAGT+TGG | + | chr1.4:19722468-19722487 | MS.gene31907:CDS | 35.0% |
| !! | TGAAGAAGAAGTATTGTTGG+TGG | - | chr1.4:19722379-19722398 | None:intergenic | 35.0% |
| AGAAACATGAATGGAGGAGT+TGG | + | chr1.4:19722504-19722523 | MS.gene31907:CDS | 40.0% | |
| ATTAACCTTTGTTGCTGCAG+AGG | - | chr1.4:19722430-19722449 | None:intergenic | 40.0% | |
| TCTGCAGCAACAAAGGTTAA+TGG | + | chr1.4:19722429-19722448 | MS.gene31907:CDS | 40.0% | |
| ! | GGACTCACTAGAGATTTTCT+TGG | + | chr1.4:19722462-19722481 | MS.gene31907:CDS | 40.0% |
| !! | AATGGAAATATTGGTGGTGG+AGG | + | chr1.4:19722567-19722586 | MS.gene31907:CDS | 40.0% |
| !! | AGAAGAAGTATTGTTGGTGG+TGG | - | chr1.4:19722376-19722395 | None:intergenic | 40.0% |
| !! | ATGGAAATATTGGTGGTGGA+GGG | + | chr1.4:19722568-19722587 | MS.gene31907:CDS | 40.0% |
| AAAGCTGTTGCTGACATGTG+AGG | - | chr1.4:19722312-19722331 | None:intergenic | 45.0% | |
| CTGACATGTGAGGAGAGATA+GGG | - | chr1.4:19722302-19722321 | None:intergenic | 45.0% | |
| GCAACAAAGGTTAATGGTGG+TGG | + | chr1.4:19722435-19722454 | MS.gene31907:CDS | 45.0% | |
| GCAGCAACAAAGGTTAATGG+TGG | + | chr1.4:19722432-19722451 | MS.gene31907:CDS | 45.0% | |
| TTCTTCCTCTGCAGCAACAA+AGG | + | chr1.4:19722422-19722441 | MS.gene31907:CDS | 45.0% | |
| ! | AGATAGGGCTTGCGCTATTT+TGG | - | chr1.4:19722287-19722306 | None:intergenic | 45.0% |
| !! | AAGGTTAATGGTGGTGGTGA+TGG | + | chr1.4:19722441-19722460 | MS.gene31907:CDS | 45.0% |
| !! | AGAAGTATTGTTGGTGGTGG+TGG | - | chr1.4:19722373-19722392 | None:intergenic | 45.0% |
| GCTGACATGTGAGGAGAGAT+AGG | - | chr1.4:19722303-19722322 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 19722207 | 19722602 | 19722207 | ID=MS.gene31907 |
| chr1.4 | mRNA | 19722207 | 19722602 | 19722207 | ID=MS.gene31907.t1;Parent=MS.gene31907 |
| chr1.4 | exon | 19722207 | 19722602 | 19722207 | ID=MS.gene31907.t1.exon1;Parent=MS.gene31907.t1 |
| chr1.4 | CDS | 19722207 | 19722602 | 19722207 | ID=cds.MS.gene31907.t1;Parent=MS.gene31907.t1 |
| Gene Sequence |
| Protein sequence |
