Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31939.t1 | XP_013457052.1 | 91.9 | 135 | 11 | 0 | 1 | 135 | 1 | 135 | 1.40E-65 | 258.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31939.t1 | Q41112 | 47.9 | 144 | 62 | 3 | 1 | 131 | 1 | 144 | 2.2e-27 | 123.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31939.t1 | G7ZZ92 | 91.9 | 135 | 11 | 0 | 1 | 135 | 1 | 135 | 9.8e-66 | 258.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055502 | MS.gene31939 | 0.812492 | 4.13E-51 | -1.69E-46 |
| MS.gene05837 | MS.gene31939 | 0.832377 | 9.78E-56 | -1.69E-46 |
| MS.gene059023 | MS.gene31939 | 0.801568 | 8.47E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene31939 | 0.815767 | 7.81E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31939.t1 | MTR_4g088915 | 91.852 | 135 | 11 | 0 | 1 | 135 | 1 | 135 | 3.45e-92 | 263 |
| MS.gene31939.t1 | MTR_2g013440 | 44.079 | 152 | 64 | 2 | 1 | 131 | 40 | 191 | 2.16e-35 | 121 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene31939.t1 | AT3G05500 | 36.207 | 174 | 68 | 4 | 1 | 131 | 37 | 210 | 3.50e-27 | 101 |
| MS.gene31939.t1 | AT2G47780 | 29.167 | 144 | 81 | 2 | 3 | 125 | 58 | 201 | 9.97e-16 | 71.2 |
| MS.gene31939.t1 | AT3G05500 | 50.000 | 54 | 27 | 0 | 78 | 131 | 85 | 138 | 1.34e-12 | 62.0 |
Find 58 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTGCCACGGGCTCGCACT+TGG | 0.318413 | 4.1:-20915443 | None:intergenic |
| TCCTTGCAATACGCGGCCTT+TGG | 0.350706 | 4.1:-20915538 | None:intergenic |
| GATGCCCGGCCTGAGCTCAT+TGG | 0.380731 | 4.1:-20915245 | None:intergenic |
| AAGTACAATGCATCGATTAT+TGG | 0.396471 | 4.1:+20915562 | MS.gene31939:CDS |
| TGCTCCAATGAGCTCAGGCC+GGG | 0.426132 | 4.1:+20915241 | MS.gene31939:CDS |
| GGCAGAGCAGACTGTGGCTT+CGG | 0.429255 | 4.1:+20915459 | MS.gene31939:CDS |
| AATCAAGACCACCACTGTGC+TGG | 0.442307 | 4.1:+20915361 | MS.gene31939:CDS |
| GAGCTCATTGGAGCACTCCT+TGG | 0.453654 | 4.1:-20915233 | None:intergenic |
| ACCACCACTGTGCTGGGCAC+AGG | 0.455798 | 4.1:+20915368 | MS.gene31939:CDS |
| CGTAGGCACCGTCTCTGAAA+TGG | 0.456825 | 4.1:+20915405 | MS.gene31939:CDS |
| AGCACAGTGGTGGTCTTGAT+TGG | 0.457759 | 4.1:-20915359 | None:intergenic |
| AGCCACAGTCTGCTCTGCCA+CGG | 0.473871 | 4.1:-20915455 | None:intergenic |
| CCCTGTGCCCAGCACAGTGG+TGG | 0.477450 | 4.1:-20915369 | None:intergenic |
| CCACCACTGTGCTGGGCACA+GGG | 0.482069 | 4.1:+20915369 | MS.gene31939:CDS |
| ACGGTGCCTACGTTGGCAAC+GGG | 0.482091 | 4.1:-20915394 | None:intergenic |
| GCCACAGTCTGCTCTGCCAC+GGG | 0.492659 | 4.1:-20915454 | None:intergenic |
| GGAGAATGCAGTTGCGCCAA+AGG | 0.496923 | 4.1:+20915522 | MS.gene31939:CDS |
| ATGCGGTTCAACGCCGTCTA+CGG | 0.502292 | 4.1:+20915205 | MS.gene31939:CDS |
| ATCAAGACCACCACTGTGCT+GGG | 0.507288 | 4.1:+20915362 | MS.gene31939:CDS |
| GATTGGTATCGGTGTGGCGG+AGG | 0.508465 | 4.1:-20915342 | None:intergenic |
| GAACAAGGTGGAACTTATCA+TGG | 0.516489 | 4.1:-20915309 | None:intergenic |
| AGGAGTGCTCCAATGAGCTC+AGG | 0.524655 | 4.1:+20915236 | MS.gene31939:CDS |
| GAAACTGTCAAGAGCGTGGT+GGG | 0.524926 | 4.1:+20915280 | MS.gene31939:CDS |
| TGTGGCTTCGGCGTGGCAGA+AGG | 0.530744 | 4.1:+20915471 | MS.gene31939:CDS |
| GTGCTCCAATGAGCTCAGGC+CGG | 0.534332 | 4.1:+20915240 | MS.gene31939:CDS |
| TCTTCAACGGTCTTGATGCC+CGG | 0.538757 | 4.1:-20915259 | None:intergenic |
| GACGGTGCCTACGTTGGCAA+CGG | 0.539764 | 4.1:-20915395 | None:intergenic |
| GTGTGGCGGAGGATCTCATC+GGG | 0.543492 | 4.1:-20915331 | None:intergenic |
| GCCAAAGGCCGCGTATTGCA+AGG | 0.549811 | 4.1:+20915537 | MS.gene31939:CDS |
| GCTCTTGACAGTTTCTTCAA+CGG | 0.549867 | 4.1:-20915272 | None:intergenic |
| GTCTCCGATGTTCCATCGAG+TGG | 0.560814 | 4.1:+20915501 | MS.gene31939:CDS |
| AGGTGGAACTTATCATGGAC+AGG | 0.561018 | 4.1:-20915304 | None:intergenic |
| CTCCTTGGCGTATCCGTAGA+CGG | 0.561808 | 4.1:-20915218 | None:intergenic |
| GATTATTGGTGCCGCAGAGA+AGG | 0.567110 | 4.1:+20915576 | MS.gene31939:CDS |
| TTACTCCAAGTGCGAGCCCG+TGG | 0.570477 | 4.1:+20915438 | MS.gene31939:CDS |
| GGAGGATCTCATCGGGAACA+AGG | 0.575130 | 4.1:-20915324 | None:intergenic |
| GTGGTGGTCTTGATTGGTAT+CGG | 0.581002 | 4.1:-20915353 | None:intergenic |
| TGCCGCAGAGAAGGAACGTA+AGG | 0.588757 | 4.1:+20915585 | MS.gene31939:CDS |
| TTCTCCACTCGATGGAACAT+CGG | 0.594689 | 4.1:-20915505 | None:intergenic |
| CCGTCTCTGAAATGGCGGTG+AGG | 0.595577 | 4.1:+20915413 | MS.gene31939:CDS |
| TTCAGAGACGGTGCCTACGT+TGG | 0.598137 | 4.1:-20915401 | None:intergenic |
| GGCCTTACGTTCCTTCTCTG+CGG | 0.599906 | 4.1:-20915587 | None:intergenic |
| CAACTGCATTCTCCACTCGA+TGG | 0.604282 | 4.1:-20915513 | None:intergenic |
| GTACTTCTCCTTGCAATACG+CGG | 0.606836 | 4.1:-20915545 | None:intergenic |
| CTTGATTGGTATCGGTGTGG+CGG | 0.609440 | 4.1:-20915345 | None:intergenic |
| AGCAGACTGTGGCTTCGGCG+TGG | 0.612873 | 4.1:+20915464 | MS.gene31939:CDS |
| AGGGCTCCCGTTGCCAACGT+AGG | 0.619019 | 4.1:+20915388 | MS.gene31939:CDS |
| CGCCGTCTACGGATACGCCA+AGG | 0.620888 | 4.1:+20915216 | MS.gene31939:CDS |
| GGTGTGGCGGAGGATCTCAT+CGG | 0.625467 | 4.1:-20915332 | None:intergenic |
| GCCCGTGGCAGAGCAGACTG+TGG | 0.630848 | 4.1:+20915453 | MS.gene31939:CDS |
| CCTCACCGCCATTTCAGAGA+CGG | 0.633755 | 4.1:-20915413 | None:intergenic |
| AAACTGTCAAGAGCGTGGTG+GGG | 0.666765 | 4.1:+20915281 | MS.gene31939:CDS |
| TGAAGAAACTGTCAAGAGCG+TGG | 0.667370 | 4.1:+20915276 | MS.gene31939:CDS |
| AGAAACTGTCAAGAGCGTGG+TGG | 0.681914 | 4.1:+20915279 | MS.gene31939:CDS |
| GAGCCCTGTGCCCAGCACAG+TGG | 0.686300 | 4.1:-20915372 | None:intergenic |
| GGTCTTGATTGGTATCGGTG+TGG | 0.686331 | 4.1:-20915348 | None:intergenic |
| AGGCACCGTCTCTGAAATGG+CGG | 0.693423 | 4.1:+20915408 | MS.gene31939:CDS |
| GGATCTCATCGGGAACAAGG+TGG | 0.702623 | 4.1:-20915321 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGTACAATGCATCGATTAT+TGG | + | chr4.1:20915562-20915581 | MS.gene31939:CDS | 30.0% |
| GAACAAGGTGGAACTTATCA+TGG | - | chr4.1:20915312-20915331 | None:intergenic | 40.0% | |
| GCTCTTGACAGTTTCTTCAA+CGG | - | chr4.1:20915275-20915294 | None:intergenic | 40.0% | |
| AGGTGGAACTTATCATGGAC+AGG | - | chr4.1:20915307-20915326 | None:intergenic | 45.0% | |
| GTACTTCTCCTTGCAATACG+CGG | - | chr4.1:20915548-20915567 | None:intergenic | 45.0% | |
| TGAAGAAACTGTCAAGAGCG+TGG | + | chr4.1:20915276-20915295 | MS.gene31939:CDS | 45.0% | |
| TTCTCCACTCGATGGAACAT+CGG | - | chr4.1:20915508-20915527 | None:intergenic | 45.0% | |
| ! | GTGGTGGTCTTGATTGGTAT+CGG | - | chr4.1:20915356-20915375 | None:intergenic | 45.0% |
| AATCAAGACCACCACTGTGC+TGG | + | chr4.1:20915361-20915380 | MS.gene31939:CDS | 50.0% | |
| ATCAAGACCACCACTGTGCT+GGG | + | chr4.1:20915362-20915381 | MS.gene31939:CDS | 50.0% | |
| CAACTGCATTCTCCACTCGA+TGG | - | chr4.1:20915516-20915535 | None:intergenic | 50.0% | |
| TCTTCAACGGTCTTGATGCC+CGG | - | chr4.1:20915262-20915281 | None:intergenic | 50.0% | |
| ! | AAACTGTCAAGAGCGTGGTG+GGG | + | chr4.1:20915281-20915300 | MS.gene31939:CDS | 50.0% |
| ! | AGAAACTGTCAAGAGCGTGG+TGG | + | chr4.1:20915279-20915298 | MS.gene31939:CDS | 50.0% |
| ! | AGCACAGTGGTGGTCTTGAT+TGG | - | chr4.1:20915362-20915381 | None:intergenic | 50.0% |
| ! | GAAACTGTCAAGAGCGTGGT+GGG | + | chr4.1:20915280-20915299 | MS.gene31939:CDS | 50.0% |
| !! | CTTGATTGGTATCGGTGTGG+CGG | - | chr4.1:20915348-20915367 | None:intergenic | 50.0% |
| !! | GATTATTGGTGCCGCAGAGA+AGG | + | chr4.1:20915576-20915595 | MS.gene31939:CDS | 50.0% |
| !! | GGTCTTGATTGGTATCGGTG+TGG | - | chr4.1:20915351-20915370 | None:intergenic | 50.0% |
| ATGCGGTTCAACGCCGTCTA+CGG | + | chr4.1:20915205-20915224 | MS.gene31939:CDS | 55.0% | |
| CCTCACCGCCATTTCAGAGA+CGG | - | chr4.1:20915416-20915435 | None:intergenic | 55.0% | |
| CTCCTTGGCGTATCCGTAGA+CGG | - | chr4.1:20915221-20915240 | None:intergenic | 55.0% | |
| GGAGAATGCAGTTGCGCCAA+AGG | + | chr4.1:20915522-20915541 | MS.gene31939:CDS | 55.0% | |
| GGAGGATCTCATCGGGAACA+AGG | - | chr4.1:20915327-20915346 | None:intergenic | 55.0% | |
| GGATCTCATCGGGAACAAGG+TGG | - | chr4.1:20915324-20915343 | None:intergenic | 55.0% | |
| GGCCTTACGTTCCTTCTCTG+CGG | - | chr4.1:20915590-20915609 | None:intergenic | 55.0% | |
| GTCTCCGATGTTCCATCGAG+TGG | + | chr4.1:20915501-20915520 | MS.gene31939:CDS | 55.0% | |
| TCCTTGCAATACGCGGCCTT+TGG | - | chr4.1:20915541-20915560 | None:intergenic | 55.0% | |
| TGCCGCAGAGAAGGAACGTA+AGG | + | chr4.1:20915585-20915604 | MS.gene31939:CDS | 55.0% | |
| TTCAGAGACGGTGCCTACGT+TGG | - | chr4.1:20915404-20915423 | None:intergenic | 55.0% | |
| ! | CGTAGGCACCGTCTCTGAAA+TGG | + | chr4.1:20915405-20915424 | MS.gene31939:CDS | 55.0% |
| ! | GAGCTCATTGGAGCACTCCT+TGG | - | chr4.1:20915236-20915255 | None:intergenic | 55.0% |
| !! | AGGAGTGCTCCAATGAGCTC+AGG | + | chr4.1:20915236-20915255 | MS.gene31939:CDS | 55.0% |
| !! | AGGCACCGTCTCTGAAATGG+CGG | + | chr4.1:20915408-20915427 | MS.gene31939:CDS | 55.0% |
| ACGGTGCCTACGTTGGCAAC+GGG | - | chr4.1:20915397-20915416 | None:intergenic | 60.0% | |
| AGCCACAGTCTGCTCTGCCA+CGG | - | chr4.1:20915458-20915477 | None:intergenic | 60.0% | |
| GACGGTGCCTACGTTGGCAA+CGG | - | chr4.1:20915398-20915417 | None:intergenic | 60.0% | |
| GCCAAAGGCCGCGTATTGCA+AGG | + | chr4.1:20915537-20915556 | MS.gene31939:CDS | 60.0% | |
| GGCAGAGCAGACTGTGGCTT+CGG | + | chr4.1:20915459-20915478 | MS.gene31939:CDS | 60.0% | |
| GTGCTCCAATGAGCTCAGGC+CGG | + | chr4.1:20915240-20915259 | MS.gene31939:CDS | 60.0% | |
| TGCTCCAATGAGCTCAGGCC+GGG | + | chr4.1:20915241-20915260 | MS.gene31939:CDS | 60.0% | |
| TTACTCCAAGTGCGAGCCCG+TGG | + | chr4.1:20915438-20915457 | MS.gene31939:CDS | 60.0% | |
| ! | GGTGTGGCGGAGGATCTCAT+CGG | - | chr4.1:20915335-20915354 | None:intergenic | 60.0% |
| ! | GTGTGGCGGAGGATCTCATC+GGG | - | chr4.1:20915334-20915353 | None:intergenic | 60.0% |
| !! | CCGTCTCTGAAATGGCGGTG+AGG | + | chr4.1:20915413-20915432 | MS.gene31939:CDS | 60.0% |
| !! | GATTGGTATCGGTGTGGCGG+AGG | - | chr4.1:20915345-20915364 | None:intergenic | 60.0% |
| ACCACCACTGTGCTGGGCAC+AGG | + | chr4.1:20915368-20915387 | MS.gene31939:CDS | 65.0% | |
| AGCAGACTGTGGCTTCGGCG+TGG | + | chr4.1:20915464-20915483 | MS.gene31939:CDS | 65.0% | |
| AGGGCTCCCGTTGCCAACGT+AGG | + | chr4.1:20915388-20915407 | MS.gene31939:CDS | 65.0% | |
| CCACCACTGTGCTGGGCACA+GGG | + | chr4.1:20915369-20915388 | MS.gene31939:CDS | 65.0% | |
| CGCCGTCTACGGATACGCCA+AGG | + | chr4.1:20915216-20915235 | MS.gene31939:CDS | 65.0% | |
| GATGCCCGGCCTGAGCTCAT+TGG | - | chr4.1:20915248-20915267 | None:intergenic | 65.0% | |
| GCCACAGTCTGCTCTGCCAC+GGG | - | chr4.1:20915457-20915476 | None:intergenic | 65.0% | |
| TGTGGCTTCGGCGTGGCAGA+AGG | + | chr4.1:20915471-20915490 | MS.gene31939:CDS | 65.0% | |
| CCCTGTGCCCAGCACAGTGG+TGG | - | chr4.1:20915372-20915391 | None:intergenic | 70.0% | |
| CTCTGCCACGGGCTCGCACT+TGG | - | chr4.1:20915446-20915465 | None:intergenic | 70.0% | |
| GAGCCCTGTGCCCAGCACAG+TGG | - | chr4.1:20915375-20915394 | None:intergenic | 70.0% | |
| GCCCGTGGCAGAGCAGACTG+TGG | + | chr4.1:20915453-20915472 | MS.gene31939:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 20915205 | 20915612 | 20915205 | ID=MS.gene31939 |
| chr4.1 | mRNA | 20915205 | 20915612 | 20915205 | ID=MS.gene31939.t1;Parent=MS.gene31939 |
| chr4.1 | exon | 20915205 | 20915612 | 20915205 | ID=MS.gene31939.t1.exon1;Parent=MS.gene31939.t1 |
| chr4.1 | CDS | 20915205 | 20915612 | 20915205 | ID=cds.MS.gene31939.t1;Parent=MS.gene31939.t1 |
| Gene Sequence |
| Protein sequence |
