AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31994

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31994.t1 MTR_4g088580 93.830 389 8 5 1 378 1 384 0.0 634
MS.gene31994.t1 MTR_2g014170 41.885 382 133 20 8 378 6 309 4.76e-47 162
MS.gene31994.t1 MTR_8g068210 62.651 83 31 0 45 127 23 105 4.08e-34 129
MS.gene31994.t1 MTR_8g479350 73.529 68 18 0 49 116 63 130 7.39e-33 126
MS.gene31994.t1 MTR_2g096740 76.562 64 15 0 54 117 62 125 1.12e-32 124
MS.gene31994.t1 MTR_2g096740 76.562 64 15 0 54 117 77 140 1.35e-32 124
MS.gene31994.t1 MTR_4g063780 62.222 90 30 1 47 132 68 157 2.40e-32 124
MS.gene31994.t1 MTR_7g059400 59.783 92 31 1 17 108 45 130 3.06e-32 124
MS.gene31994.t1 MTR_2g093220 83.333 60 10 0 49 108 35 94 3.20e-32 123
MS.gene31994.t1 MTR_8g027295 53.333 105 39 1 43 147 13 107 3.56e-32 122
MS.gene31994.t1 MTR_4g022370 48.276 116 60 0 47 162 75 190 4.21e-32 124
MS.gene31994.t1 MTR_4g461080 85.965 57 8 0 50 106 25 81 8.34e-32 121
MS.gene31994.t1 MTR_3g091820 71.233 73 16 1 41 108 25 97 2.12e-31 121
MS.gene31994.t1 MTR_8g079060 65.789 76 26 0 50 125 25 100 3.14e-31 119
MS.gene31994.t1 MTR_3g090430 55.882 102 38 2 15 110 7 107 8.22e-31 120
MS.gene31994.t1 MTR_2g013370 66.667 78 19 2 39 110 25 101 4.40e-30 117
MS.gene31994.t1 MTR_2g014060 77.049 61 14 0 45 105 16 76 4.74e-30 118
MS.gene31994.t1 MTR_4g089095 72.973 74 19 1 38 111 34 106 1.59e-29 115
MS.gene31994.t1 MTR_4g089095 80.000 60 11 1 52 111 14 72 2.02e-29 114
MS.gene31994.t1 MTR_5g031440 79.630 54 11 0 51 104 40 93 2.94e-29 116
MS.gene31994.t1 MTR_7g024670 71.875 64 18 0 53 116 76 139 3.05e-29 116
MS.gene31994.t1 MTR_1g056810 79.310 58 12 0 51 108 9 66 3.94e-29 114
MS.gene31994.t1 MTR_1g077600 67.606 71 21 1 40 108 27 97 5.09e-29 114
MS.gene31994.t1 MTR_1g077600 67.606 71 21 1 40 108 27 97 5.34e-29 114
MS.gene31994.t1 MTR_8g015840 78.571 56 12 0 50 105 16 71 9.21e-29 112
MS.gene31994.t1 MTR_3g077750 72.131 61 17 0 44 104 51 111 2.40e-28 113
MS.gene31994.t1 MTR_4g109980 81.132 53 10 0 52 104 38 90 2.53e-28 113
MS.gene31994.t1 MTR_2g059540 84.000 50 8 0 53 102 30 79 1.05e-26 105
MS.gene31994.t1 MTR_3g435480 65.625 64 22 0 44 107 126 189 2.77e-26 109
MS.gene31994.t1 MTR_4g082060 62.121 66 25 0 44 109 112 177 8.18e-26 108
MS.gene31994.t1 MTR_7g086780 63.077 65 24 0 45 109 90 154 1.58e-25 107
MS.gene31994.t1 MTR_7g010950 65.079 63 22 0 45 107 126 188 2.32e-25 107
MS.gene31994.t1 MTR_6g012450 67.213 61 20 0 47 107 129 189 9.45e-25 105
MS.gene31994.t1 MTR_8g044220 45.918 98 43 2 13 108 83 172 1.06e-24 105
MS.gene31994.t1 MTR_2g016030 50.000 94 38 2 44 137 37 121 2.39e-24 98.2
MS.gene31994.t1 MTR_6g027450 45.745 94 41 2 16 109 61 144 2.97e-24 102
MS.gene31994.t1 MTR_6g027460 58.462 65 27 0 45 109 87 151 6.32e-24 102
MS.gene31994.t1 MTR_5g041420 63.333 60 22 0 48 107 132 191 5.20e-23 98.6
MS.gene31994.t1 MTR_5g041530 64.407 59 21 0 49 107 135 193 3.51e-21 94.4
MS.gene31994.t1 MTR_5g041380 67.857 56 18 0 52 107 125 180 5.61e-21 93.6
MS.gene31994.t1 MTR_5g041400 70.370 54 16 0 54 107 119 172 8.27e-21 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31994.t1 AT1G28310 37.202 336 149 16 54 378 27 311 2.94e-36 134
MS.gene31994.t1 AT1G28310 37.202 336 149 16 54 378 51 335 3.26e-36 135
MS.gene31994.t1 AT5G65590 63.043 92 28 1 24 115 17 102 5.99e-36 134
MS.gene31994.t1 AT5G60200 81.967 61 11 0 48 108 49 109 5.41e-34 127
MS.gene31994.t1 AT2G37590 65.753 73 25 0 38 110 73 145 3.03e-32 124
MS.gene31994.t1 AT3G61850 45.455 143 59 4 51 174 71 213 4.44e-32 122
MS.gene31994.t1 AT3G61850 44.828 145 61 4 49 174 57 201 4.76e-32 122
MS.gene31994.t1 AT3G61850 44.828 145 61 4 49 174 57 201 4.76e-32 122
MS.gene31994.t1 AT3G55370 61.176 85 33 0 26 110 48 132 1.67e-31 122
MS.gene31994.t1 AT5G02460 82.759 58 10 0 51 108 92 149 2.21e-31 123
MS.gene31994.t1 AT3G55370 61.176 85 33 0 26 110 48 132 3.00e-31 122
MS.gene31994.t1 AT3G55370 61.176 85 33 0 26 110 93 177 3.12e-31 122
MS.gene31994.t1 AT2G28810 79.310 58 12 0 48 105 71 128 4.23e-31 120
MS.gene31994.t1 AT1G21340 64.557 79 25 1 39 114 20 98 4.72e-31 119
MS.gene31994.t1 AT5G60850 70.667 75 19 1 52 123 49 123 4.78e-31 120
MS.gene31994.t1 AT1G07640 46.032 126 64 1 35 160 2 123 4.91e-31 119
MS.gene31994.t1 AT3G50410 67.532 77 23 2 37 111 11 87 5.64e-31 119
MS.gene31994.t1 AT2G28810 79.310 58 12 0 48 105 88 145 6.51e-31 120
MS.gene31994.t1 AT1G07640 48.673 113 51 2 3 108 19 131 6.69e-31 120
MS.gene31994.t1 AT1G07640 48.673 113 51 2 3 108 27 139 7.15e-31 120
MS.gene31994.t1 AT3G45610 77.586 58 13 0 51 108 37 94 1.02e-30 117
MS.gene31994.t1 AT2G28510 82.456 57 10 0 52 108 46 102 1.27e-30 119
MS.gene31994.t1 AT1G51700 60.215 93 28 3 17 108 4 88 1.81e-30 115
MS.gene31994.t1 AT3G61850 53.271 107 41 2 49 146 57 163 2.47e-30 118
MS.gene31994.t1 AT3G61850 53.271 107 41 2 49 146 69 175 3.38e-30 118
MS.gene31994.t1 AT3G52440 81.818 55 10 0 56 110 27 81 2.06e-29 114
MS.gene31994.t1 AT3G52440 81.818 55 10 0 56 110 46 100 3.51e-29 114
MS.gene31994.t1 AT3G21270 84.906 53 8 0 54 106 29 81 5.52e-29 112
MS.gene31994.t1 AT4G00940 74.603 63 16 0 51 113 65 127 5.95e-29 114
MS.gene31994.t1 AT4G00940 74.603 63 16 0 51 113 65 127 5.95e-29 114
MS.gene31994.t1 AT4G00940 74.603 63 16 0 51 113 65 127 5.95e-29 114
MS.gene31994.t1 AT4G24060 75.000 60 15 0 49 108 48 107 7.29e-29 115
MS.gene31994.t1 AT4G21050 60.000 80 32 0 47 126 17 96 2.38e-28 110
MS.gene31994.t1 AT5G66940 56.977 86 33 2 50 132 28 112 2.66e-28 110
MS.gene31994.t1 AT4G38000 79.310 58 11 1 51 107 38 95 8.28e-28 110
MS.gene31994.t1 AT5G62940 69.355 62 19 0 43 104 62 123 3.79e-27 111
MS.gene31994.t1 AT3G47500 66.667 66 22 0 42 107 98 163 2.81e-26 109
MS.gene31994.t1 AT5G62430 44.860 107 49 2 1 107 11 107 1.01e-25 105
MS.gene31994.t1 AT1G64620 74.545 55 14 0 49 103 44 98 2.54e-25 105
MS.gene31994.t1 AT2G46590 78.431 51 11 0 51 101 65 115 3.01e-25 105
MS.gene31994.t1 AT2G46590 78.431 51 11 0 51 101 77 127 3.99e-25 105
MS.gene31994.t1 AT5G39660 68.852 61 19 0 47 107 131 191 4.19e-25 106
MS.gene31994.t1 AT5G39660 68.852 61 19 0 47 107 131 191 4.19e-25 106
MS.gene31994.t1 AT5G39660 68.852 61 19 0 47 107 131 191 4.19e-25 106
MS.gene31994.t1 AT1G69570 60.294 68 27 0 47 114 125 192 2.37e-24 103
MS.gene31994.t1 AT1G26790 63.934 61 22 0 47 107 104 164 2.44e-24 103
MS.gene31994.t1 AT1G47655 72.549 51 14 0 52 102 27 77 7.35e-24 98.6
MS.gene31994.t1 AT1G29160 48.315 89 44 1 39 125 45 133 1.89e-23 96.3
MS.gene31994.t1 AT2G34140 53.086 81 31 1 27 107 38 111 2.43e-23 95.9
MS.gene31994.t1 AT4G21040 57.143 77 30 2 56 131 27 101 3.01e-23 97.4
MS.gene31994.t1 AT4G21080 65.517 58 19 1 56 112 27 84 3.22e-21 92.0
MS.gene31994.t1 AT4G21030 46.053 76 41 0 56 131 23 98 3.64e-14 70.9

Find 68 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATCAATTCTCTTCTTTA+TGG 0.060643 4.3:-24665699 MS.gene31994:CDS
CAGATTCTTCTTCTCTTTAT+TGG 0.115189 4.3:-24665164 MS.gene31994:CDS
AACAGCAACAACAAGCTTTA+AGG 0.128070 4.3:-24666022 MS.gene31994:CDS
TCAAAACCAGATGGAGAATA+TGG 0.176496 4.3:-24665193 MS.gene31994:CDS
CTTCTTCAAGTGGTGGAAAT+AGG 0.186986 4.3:-24666076 MS.gene31994:CDS
GAATTATGACCAATATTATT+AGG 0.234994 4.3:+24665069 None:intergenic
CAGGTGAATGGTCTTGGATT+AGG 0.278693 4.3:-24665567 MS.gene31994:CDS
CATAAAGAAGAGAATTGATA+TGG 0.297144 4.3:+24665700 None:intergenic
GCAAAGCCTGTAAAAGATAT+TGG 0.299579 4.3:-24665923 MS.gene31994:CDS
AACAACTACAGTTCCATCTT+TGG 0.305663 4.3:-24665477 MS.gene31994:CDS
TCATCATGAGGCTGGGATAA+TGG 0.306350 4.3:-24665304 MS.gene31994:CDS
TGCTGCAGTGTTGTTGTTGT+TGG 0.309230 4.3:+24665128 None:intergenic
GATGATTACAGAAATGGATT+TGG 0.345610 4.3:-24665510 MS.gene31994:CDS
CATCATGATGATTACAGAAA+TGG 0.360895 4.3:-24665516 MS.gene31994:CDS
CAACAGGTTTGAGTAGTGTT+TGG 0.362182 4.3:-24665107 MS.gene31994:CDS
AGAGTTTCAAGTTCTTCTTC+TGG 0.369826 4.3:-24665618 MS.gene31994:CDS
TTCTTCAAGTGGTGGAAATA+GGG 0.372187 4.3:-24666075 MS.gene31994:CDS
TTAAGTAGAGAAGCCATAAC+TGG 0.397152 4.3:+24665408 None:intergenic
CAGCCTCAGGTGAATGGTCT+TGG 0.403606 4.3:-24665573 MS.gene31994:CDS
AAGAGAATTGATATGGTTTG+AGG 0.411504 4.3:+24665707 None:intergenic
GATCACCAACCTAATAATAT+TGG 0.414706 4.3:-24665078 MS.gene31994:CDS
CTCATAGAGACCAAAATGTT+TGG 0.415114 4.3:-24666368 None:intergenic
TAGAAGCAATATCAAAACTA+TGG 0.419426 4.3:+24665730 None:intergenic
ATGTTTGAAGAAGAGTGATT+TGG 0.420876 4.3:+24665831 None:intergenic
TAGGTGAAGGACTGCAAAAT+AGG 0.421549 4.3:-24665263 MS.gene31994:CDS
CAAAACCAGATGGAGAATAT+GGG 0.434787 4.3:-24665192 MS.gene31994:CDS
CAGGCTTTGCAGAAGTGTCT+TGG 0.440181 4.3:+24665936 None:intergenic
AACAACAACACTGCAGCAAC+AGG 0.445401 4.3:-24665123 MS.gene31994:CDS
AAGTGGTGGAAATAGGGTTA+TGG 0.446908 4.3:-24666069 MS.gene31994:CDS
AGATGATCATAAGGTTTCAA+TGG 0.457556 4.3:-24666099 MS.gene31994:CDS
GGCCTCTACTACTCCAGTTA+TGG 0.463925 4.3:-24665421 MS.gene31994:CDS
GGGTTTGAATTAGCACTAGT+AGG 0.464115 4.3:+24665774 None:intergenic
GAATTGAACCTTGTTGCAAA+AGG 0.464424 4.3:+24665645 None:intergenic
ACAAACCCATATTCTCCATC+TGG 0.464554 4.3:+24665187 None:intergenic
TATGACCAATATTATTAGGT+TGG 0.469646 4.3:+24665073 None:intergenic
TTTCTGCAACCACCACCAAC+AGG 0.472277 4.3:+24665873 None:intergenic
ATCCCAGCCTCATGATGAAA+AGG 0.481394 4.3:+24665309 None:intergenic
GCTGCTGCTGGTGGAATGAA+AGG 0.490952 4.3:-24665339 MS.gene31994:CDS
GGCTGAGATAGATGATCATA+AGG 0.493744 4.3:-24666108 MS.gene31994:CDS
TGAAGGACTGCAAAATAGGT+TGG 0.500845 4.3:-24665259 MS.gene31994:CDS
GTTGTTGTTGTTGAGAAGGA+GGG 0.513508 4.3:+24665754 None:intergenic
CAGCAGCAGCCTCAGGTGAA+TGG 0.518167 4.3:-24665579 MS.gene31994:CDS
CTTAGAAACGTTCCTGTTGG+TGG 0.520182 4.3:-24665885 MS.gene31994:CDS
AACTGGAGTAGTAGAGGCCA+TGG 0.527816 4.3:+24665425 None:intergenic
TGTTGTTGTTGTTGAGAAGG+AGG 0.530854 4.3:+24665753 None:intergenic
AGTAGAAGAAGAACCAAAGA+TGG 0.540629 4.3:+24665464 None:intergenic
TGGTGTTGTTGTTGTTGAGA+AGG 0.544856 4.3:+24665750 None:intergenic
CTAGTAGGAGTTGAAGAAGA+AGG 0.548358 4.3:+24665789 None:intergenic
TCTTCAGCAGCAGCAGCCTC+AGG 0.551095 4.3:-24665586 MS.gene31994:CDS
AGTAACAATATGAATGCTAA+TGG 0.560213 4.3:-24665228 MS.gene31994:CDS
TGTAAAAGATATTGGACAAG+AGG 0.561250 4.3:-24665915 MS.gene31994:CDS
TGCAAAATAGGTTGGATCAG+TGG 0.563033 4.3:-24665251 MS.gene31994:CDS
GTTTCAATGGCTTCTTCAAG+TGG 0.563411 4.3:-24666086 MS.gene31994:CDS
AGCCATAACTGGAGTAGTAG+AGG 0.573379 4.3:+24665419 None:intergenic
AGAAACGTTCCTGTTGGTGG+TGG 0.578028 4.3:-24665882 MS.gene31994:CDS
TGTTGAAGAAGCTCTTGACC+TGG 0.579160 4.3:+24666041 None:intergenic
AAAAGATATTGGACAAGAGG+TGG 0.582761 4.3:-24665912 MS.gene31994:CDS
TCAATGGCTTCTTCAAGTGG+TGG 0.584214 4.3:-24666083 MS.gene31994:CDS
TAACTGTGTTGATGAGAAAA+CGG 0.594803 4.3:-24666345 MS.gene31994:CDS
GATTCAAAAGAAGTGAAACT+AGG 0.595250 4.3:-24665282 MS.gene31994:CDS
TTCAACTAATGATAACTCCA+TGG 0.607734 4.3:-24665442 MS.gene31994:CDS
ACACTTAGAAACGTTCCTGT+TGG 0.611337 4.3:-24665888 MS.gene31994:CDS
CACTTGTAACAAATTGACAC+AGG 0.623733 4.3:-24666318 MS.gene31994:intron
AAAGAAGTGAAACTAGGTGA+AGG 0.626336 4.3:-24665276 MS.gene31994:CDS
AACAATATGAATGCTAATGG+TGG 0.654745 4.3:-24665225 MS.gene31994:CDS
GCTGCTGGTGGAATGAAAGG+TGG 0.658894 4.3:-24665336 MS.gene31994:CDS
GTGTTTGATGAGTCACAACG+TGG 0.698952 4.3:+24665996 None:intergenic
AATCCAAGACCATTCACCTG+AGG 0.720277 4.3:+24665570 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAAATTAGATATTTTTTTAA+TGG + chr4.3:24665257-24665276 None:intergenic 10.0%
!! GAATTATGACCAATATTATT+AGG + chr4.3:24666337-24666356 None:intergenic 20.0%
! AGTAACAATATGAATGCTAA+TGG - chr4.3:24666175-24666194 MS.gene31994:intron 25.0%
! CATAAAGAAGAGAATTGATA+TGG + chr4.3:24665706-24665725 None:intergenic 25.0%
! CATATCAATTCTCTTCTTTA+TGG - chr4.3:24665704-24665723 MS.gene31994:CDS 25.0%
! TAGAAGCAATATCAAAACTA+TGG + chr4.3:24665676-24665695 None:intergenic 25.0%
! TATGACCAATATTATTAGGT+TGG + chr4.3:24666333-24666352 None:intergenic 25.0%
!!! AAATTTTGTTGTTTTCCTCT+TGG - chr4.3:24665164-24665183 MS.gene31994:CDS 25.0%
!!! AAGAAAGAGAAAGTGTTTTA+AGG + chr4.3:24665224-24665243 None:intergenic 25.0%
!!! TTGTTTTTCTTATCCTTTGT+AGG - chr4.3:24665274-24665293 MS.gene31994:CDS 25.0%
AACAATATGAATGCTAATGG+TGG - chr4.3:24666178-24666197 MS.gene31994:intron 30.0%
AAGAGAATTGATATGGTTTG+AGG + chr4.3:24665699-24665718 None:intergenic 30.0%
AGATGATCATAAGGTTTCAA+TGG - chr4.3:24665304-24665323 MS.gene31994:CDS 30.0%
ATGTTTGAAGAAGAGTGATT+TGG + chr4.3:24665575-24665594 None:intergenic 30.0%
CAGATTCTTCTTCTCTTTAT+TGG - chr4.3:24666239-24666258 MS.gene31994:intron 30.0%
CATCATGATGATTACAGAAA+TGG - chr4.3:24665887-24665906 MS.gene31994:CDS 30.0%
GATCACCAACCTAATAATAT+TGG - chr4.3:24666325-24666344 MS.gene31994:CDS 30.0%
GATTCAAAAGAAGTGAAACT+AGG - chr4.3:24666121-24666140 MS.gene31994:intron 30.0%
GGAAAACAACAAAATTTGAG+TGG + chr4.3:24665161-24665180 None:intergenic 30.0%
TGTAAAAGATATTGGACAAG+AGG - chr4.3:24665488-24665507 MS.gene31994:CDS 30.0%
TTCAACTAATGATAACTCCA+TGG - chr4.3:24665961-24665980 MS.gene31994:CDS 30.0%
! GATGATTACAGAAATGGATT+TGG - chr4.3:24665893-24665912 MS.gene31994:CDS 30.0%
! TAACTGTGTTGATGAGAAAA+CGG - chr4.3:24665058-24665077 MS.gene31994:CDS 30.0%
! TCTTGTCCAATATCTTTTAC+AGG + chr4.3:24665489-24665508 None:intergenic 30.0%
!! TTGGATTAGGTTTTTCATCT+GGG - chr4.3:24665849-24665868 MS.gene31994:CDS 30.0%
!!! AAAGAGAAAGTGTTTTAAGG+TGG + chr4.3:24665221-24665240 None:intergenic 30.0%
AAAAGATATTGGACAAGAGG+TGG - chr4.3:24665491-24665510 MS.gene31994:CDS 35.0%
AAAGAAGTGAAACTAGGTGA+AGG - chr4.3:24666127-24666146 MS.gene31994:intron 35.0%
AACAACTACAGTTCCATCTT+TGG - chr4.3:24665926-24665945 MS.gene31994:CDS 35.0%
AACAGCAACAACAAGCTTTA+AGG - chr4.3:24665381-24665400 MS.gene31994:CDS 35.0%
AATAGGGTTATGGAAAAACC+AGG - chr4.3:24665344-24665363 MS.gene31994:CDS 35.0%
AGTAGAAGAAGAACCAAAGA+TGG + chr4.3:24665942-24665961 None:intergenic 35.0%
CAAAACCAGATGGAGAATAT+GGG - chr4.3:24666211-24666230 MS.gene31994:intron 35.0%
CACTTGTAACAAATTGACAC+AGG - chr4.3:24665085-24665104 MS.gene31994:CDS 35.0%
GAATTGAACCTTGTTGCAAA+AGG + chr4.3:24665761-24665780 None:intergenic 35.0%
GCAAAGCCTGTAAAAGATAT+TGG - chr4.3:24665480-24665499 MS.gene31994:CDS 35.0%
TCAAAACCAGATGGAGAATA+TGG - chr4.3:24666210-24666229 MS.gene31994:intron 35.0%
TTAAGTAGAGAAGCCATAAC+TGG + chr4.3:24665998-24666017 None:intergenic 35.0%
! TGAACTTTCCTTTTGCAACA+AGG - chr4.3:24665750-24665769 MS.gene31994:CDS 35.0%
! TTCTTCAAGTGGTGGAAATA+GGG - chr4.3:24665328-24665347 MS.gene31994:CDS 35.0%
!! AGAGTTTCAAGTTCTTCTTC+TGG - chr4.3:24665785-24665804 MS.gene31994:CDS 35.0%
!! CTTGGATTAGGTTTTTCATC+TGG - chr4.3:24665848-24665867 MS.gene31994:CDS 35.0%
!! TGGATTAGGTTTTTCATCTG+GGG - chr4.3:24665850-24665869 MS.gene31994:CDS 35.0%
!!! TGTTGCTTTTCAAAACCAGA+TGG - chr4.3:24666201-24666220 MS.gene31994:intron 35.0%
ACAAACCCATATTCTCCATC+TGG + chr4.3:24666219-24666238 None:intergenic 40.0%
ACACTTAGAAACGTTCCTGT+TGG - chr4.3:24665515-24665534 MS.gene31994:CDS 40.0%
CAACAGGTTTGAGTAGTGTT+TGG - chr4.3:24666296-24666315 MS.gene31994:intron 40.0%
CTAGTAGGAGTTGAAGAAGA+AGG + chr4.3:24665617-24665636 None:intergenic 40.0%
GATAGAGAAAGAGAGTGAGA+TGG + chr4.3:24665113-24665132 None:intergenic 40.0%
GGCTGAGATAGATGATCATA+AGG - chr4.3:24665295-24665314 MS.gene31994:CDS 40.0%
GGGTTTGAATTAGCACTAGT+AGG + chr4.3:24665632-24665651 None:intergenic 40.0%
GTTTCAATGGCTTCTTCAAG+TGG - chr4.3:24665317-24665336 MS.gene31994:CDS 40.0%
TAGGTGAAGGACTGCAAAAT+AGG - chr4.3:24666140-24666159 MS.gene31994:intron 40.0%
TCATCTATCTCAGCCTACAA+AGG + chr4.3:24665290-24665309 None:intergenic 40.0%
TGAAGGACTGCAAAATAGGT+TGG - chr4.3:24666144-24666163 MS.gene31994:intron 40.0%
TGGCTATATTCTGTTCCAAG+AGG + chr4.3:24665182-24665201 None:intergenic 40.0%
! AAGTGGTGGAAATAGGGTTA+TGG - chr4.3:24665334-24665353 MS.gene31994:CDS 40.0%
! ATCCTTTTCATCATGAGGCT+GGG - chr4.3:24666092-24666111 MS.gene31994:CDS 40.0%
! CTTCTTCAAGTGGTGGAAAT+AGG - chr4.3:24665327-24665346 MS.gene31994:CDS 40.0%
! GTTGTTGTTGTTGAGAAGGA+GGG + chr4.3:24665652-24665671 None:intergenic 40.0%
! TGCAAAATAGGTTGGATCAG+TGG - chr4.3:24666152-24666171 MS.gene31994:intron 40.0%
! TGGTGATCCTTTTCATCATG+AGG - chr4.3:24666087-24666106 MS.gene31994:CDS 40.0%
! TGTTGTTGTTGTTGAGAAGG+AGG + chr4.3:24665653-24665672 None:intergenic 40.0%
!! TGGTGTTGTTGTTGTTGAGA+AGG + chr4.3:24665656-24665675 None:intergenic 40.0%
AACAACAACACTGCAGCAAC+AGG - chr4.3:24666280-24666299 MS.gene31994:intron 45.0%
AATCCAAGACCATTCACCTG+AGG + chr4.3:24665836-24665855 None:intergenic 45.0%
AGCCATAACTGGAGTAGTAG+AGG + chr4.3:24665987-24666006 None:intergenic 45.0%
ATCCCAGCCTCATGATGAAA+AGG + chr4.3:24666097-24666116 None:intergenic 45.0%
CTTAGAAACGTTCCTGTTGG+TGG - chr4.3:24665518-24665537 MS.gene31994:CDS 45.0%
GTGTTTGATGAGTCACAACG+TGG + chr4.3:24665410-24665429 None:intergenic 45.0%
TCAATGGCTTCTTCAAGTGG+TGG - chr4.3:24665320-24665339 MS.gene31994:CDS 45.0%
TCATCATGAGGCTGGGATAA+TGG - chr4.3:24666099-24666118 MS.gene31994:CDS 45.0%
TGTTGAAGAAGCTCTTGACC+TGG + chr4.3:24665365-24665384 None:intergenic 45.0%
! CAGGTGAATGGTCTTGGATT+AGG - chr4.3:24665836-24665855 MS.gene31994:CDS 45.0%
! GATCCTTTTCATCATGAGGC+TGG - chr4.3:24666091-24666110 MS.gene31994:CDS 45.0%
! TGCTGCAGTGTTGTTGTTGT+TGG + chr4.3:24666278-24666297 None:intergenic 45.0%
!!! GTGGTGCATGTTTTAGTCAG+TGG + chr4.3:24665202-24665221 None:intergenic 45.0%
AACTGGAGTAGTAGAGGCCA+TGG + chr4.3:24665981-24666000 None:intergenic 50.0%
GGCCTCTACTACTCCAGTTA+TGG - chr4.3:24665982-24666001 MS.gene31994:CDS 50.0%
TTTCTGCAACCACCACCAAC+AGG + chr4.3:24665533-24665552 None:intergenic 50.0%
! AGAAACGTTCCTGTTGGTGG+TGG - chr4.3:24665521-24665540 MS.gene31994:CDS 50.0%
!! CAGGCTTTGCAGAAGTGTCT+TGG + chr4.3:24665470-24665489 None:intergenic 50.0%
CAGCCTCAGGTGAATGGTCT+TGG - chr4.3:24665830-24665849 MS.gene31994:CDS 55.0%
GCTGCTGCTGGTGGAATGAA+AGG - chr4.3:24666064-24666083 MS.gene31994:CDS 55.0%
GCTGCTGGTGGAATGAAAGG+TGG - chr4.3:24666067-24666086 MS.gene31994:CDS 55.0%
CAGCAGCAGCCTCAGGTGAA+TGG - chr4.3:24665824-24665843 MS.gene31994:CDS 60.0%
TCTTCAGCAGCAGCAGCCTC+AGG - chr4.3:24665817-24665836 MS.gene31994:CDS 60.0%
GCTGCTGCTGCTGCTGCTGC+TGG - chr4.3:24666052-24666071 MS.gene31994:CDS 70.0%
GCTGCTGCTGCTGCTGCTGG+TGG - chr4.3:24666055-24666074 MS.gene31994:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 24665050 24666375 24665050 ID=MS.gene31994
chr4.3 mRNA 24665050 24666375 24665050 ID=MS.gene31994.t1;Parent=MS.gene31994
chr4.3 exon 24666319 24666375 24666319 ID=MS.gene31994.t1.exon1;Parent=MS.gene31994.t1
chr4.3 CDS 24666319 24666375 24666319 ID=cds.MS.gene31994.t1;Parent=MS.gene31994.t1
chr4.3 exon 24665050 24666129 24665050 ID=MS.gene31994.t1.exon2;Parent=MS.gene31994.t1
chr4.3 CDS 24665050 24666129 24665050 ID=cds.MS.gene31994.t1;Parent=MS.gene31994.t1
Gene Sequence

>MS.gene31994

ATGTTTGGTAACTGTGTTGATGAGAAAACGGTAATCACTTGTAACAAATTGACACAGGTTCCATCTCACTCTCTTTCTCTATCTCAATTATTAGACAAAAATACATGACCACTCAAATTTTGTTGTTTTCCTCTTGGAACAGAATATAGCCACTGACTAAAACATGCACCACCTTAAAACACTTTCTCTTTCTTCTTCTTTTTTCCATTAAAAAAATATCTAATTTGTTTTTCTTATCCTTTGTAGGCTGAGATAGATGATCATAAGGTTTCAATGGCTTCTTCAAGTGGTGGAAATAGGGTTATGGAAAAACCAGGTCAAGAGCTTCTTCAACAGCAACAACAAGCTTTAAGGTGTCCACGTTGTGACTCATCAAACACTAAATTCTGTTACTACAATAACTACAGTTTATCACAACCAAGACACTTCTGCAAAGCCTGTAAAAGATATTGGACAAGAGGTGGAACACTTAGAAACGTTCCTGTTGGTGGTGGTTGCAGAAAAAACAAACGTCTTAAACGACCAAATCACTCTTCTTCAAACATTGATACTGCTACTGCTTCTCCTTCTTCTTCAACTCCTACTAGTGCTAATTCAAACCCTCCTTCTCAACAACAACAACACCATAGTTTTGATATTGCTTCTACCTCAAACCATATCAATTCTCTTCTTTATGGTAACTCTTCTTGTCATGATGTGATGAACTTTCCTTTTGCAACAAGGTTCAATTCTACTAGAGTTTCAAGTTCTTCTTCTGGTTATGATCATCTTCAGCAGCAGCAGCCTCAGGTGAATGGTCTTGGATTAGGTTTTTCATCTGGGGTAGTTAATCTTAATCATCATGATGATTACAGAAATGGATTTGGTTCAAATAATAACAACTACAGTTCCATCTTTGGTTCTTCTTCTACTTCAACTAATGATAACTCCATGGCCTCTACTACTCCAGTTATGGCTTCTCTACTTAACTCTACACTACTACAACAGAAATTCATGAGTACAGCTGCTGCTGCTGCTGCTGCTGGTGGAATGAAAGGTGGTGATCCTTTTCATCATGAGGCTGGGATAATGGATTCAAAAGAAGTGAAACTAGGTGAAGGACTGCAAAATAGGTTGGATCAGTGGAGTAACAATATGAATGCTAATGGTGGTGTTGCTTTTCAAAACCAGATGGAGAATATGGGTTTGTCAGATTCTTCTTCTCTTTATTGGAATAACAACAACACCAACAACAACAACACTGCAGCAACAGGTTTGAGTAGTGTTTGGAGTATTGATCACCAACCTAATAATATTGGTCATAATTCAGTTACTTCTCTGATCTAG
Protein sequence

>MS.gene31994.t1

MFGNCVDEKTVITCNKLTQAEIDDHKVSMASSSGGNRVMEKPGQELLQQQQQALRCPRCDSSNTKFCYYNNYSLSQPRHFCKACKRYWTRGGTLRNVPVGGGCRKNKRLKRPNHSSSNIDTATASPSSSTPTSANSNPPSQQQQHHSFDIASTSNHINSLLYGNSSCHDVMNFPFATRFNSTRVSSSSSGYDHLQQQQPQVNGLGLGFSSGVVNLNHHDDYRNGFGSNNNNYSSIFGSSSTSTNDNSMASTTPVMASLLNSTLLQQKFMSTAAAAAAAGGMKGGDPFHHEAGIMDSKEVKLGEGLQNRLDQWSNNMNANGGVAFQNQMENMGLSDSSSLYWNNNNTNNNNTAATGLSSVWSIDHQPNNIGHNSVTSLI