Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32171.t1 | XP_013468665.1 | 95.5 | 336 | 15 | 0 | 1 | 336 | 309 | 644 | 1.40E-167 | 599 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32171.t1 | A0A072VLI3 | 95.5 | 336 | 15 | 0 | 1 | 336 | 309 | 644 | 1.0e-167 | 599.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055465 | MS.gene32171 | 0.810593 | 1.07E-50 | -1.69E-46 |
| MS.gene055468 | MS.gene32171 | 0.828326 | 9.56E-55 | -1.69E-46 |
| MS.gene055469 | MS.gene32171 | 0.821966 | 3.05E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32171.t1 | MTR_1g073930 | 95.238 | 336 | 16 | 0 | 1 | 336 | 309 | 644 | 0.0 | 611 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32171.t1 | AT2G43990 | 33.475 | 236 | 133 | 7 | 7 | 227 | 316 | 542 | 3.58e-18 | 85.9 |
Find 0 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATCAAAAAACCAAACTATGA+TGG | - | 8581:17067-17086 | MS.gene32171:CDS | 25.0% |
| ! | GAAAACAATATGGAATCAAA+AGG | + | 8581:16488-16507 | MS.gene32171:intergenic | 25.0% |
| ! | GCATAGTTAAACAATCAAAT+TGG | + | 8581:16790-16809 | MS.gene32171:intergenic | 25.0% |
| ! | TCAAAAAACCAAACTATGAT+GGG | - | 8581:17068-17087 | MS.gene32171:CDS | 25.0% |
| !!! | AATTTTTTTGAACTGGATGA+TGG | + | 8581:16332-16351 | MS.gene32171:intergenic | 25.0% |
| !!! | ATTCCAGAATTTTTTTGAAC+TGG | + | 8581:16339-16358 | MS.gene32171:intergenic | 25.0% |
| AAAAAACCAAACTATGATGG+GGG | - | 8581:17070-17089 | MS.gene32171:CDS | 30.0% | |
| AACTGAACGAATTAAAGTCA+GGG | + | 8581:16697-16716 | MS.gene32171:intergenic | 30.0% | |
| AATCTCAAATGAGGATATCA+TGG | - | 8581:16903-16922 | MS.gene32171:CDS | 30.0% | |
| ATTGTAGAACTAGATAACCA+AGG | + | 8581:16869-16888 | MS.gene32171:intergenic | 30.0% | |
| CAAAAAACCAAACTATGATG+GGG | - | 8581:17069-17088 | MS.gene32171:CDS | 30.0% | |
| CAGCAAACTTCCAAAAAAAT+TGG | - | 8581:16829-16848 | MS.gene32171:CDS | 30.0% | |
| CATCCAGTTCAAAAAAATTC+TGG | - | 8581:16333-16352 | MS.gene32171:CDS | 30.0% | |
| GTTAATGAACCAAGTTAATG+AGG | - | 8581:16934-16953 | MS.gene32171:CDS | 30.0% | |
| TAACTGAACGAATTAAAGTC+AGG | + | 8581:16698-16717 | MS.gene32171:intergenic | 30.0% | |
| ! | ATTGGCAAAGAGATATTTTC+TGG | - | 8581:17133-17152 | MS.gene32171:CDS | 30.0% |
| ! | CTGAGATCAATTGATTCATA+AGG | + | 8581:16812-16831 | MS.gene32171:intergenic | 30.0% |
| !! | GTGATAGTTCTAAAACTGTT+TGG | - | 8581:16363-16382 | MS.gene32171:CDS | 30.0% |
| !! | GTTATCTAGTTCTACAATAG+AGG | - | 8581:16871-16890 | MS.gene32171:CDS | 30.0% |
| ACTCATCTACCTCATTAACT+TGG | + | 8581:16946-16965 | MS.gene32171:intergenic | 35.0% | |
| ATGAGTTCTTCAATTTCTCC+AGG | - | 8581:16440-16459 | MS.gene32171:CDS | 35.0% | |
| ATGGATCACAATCTCAAATG+AGG | - | 8581:16894-16913 | MS.gene32171:CDS | 35.0% | |
| CTAATTGAATTCAGCGAGAA+TGG | + | 8581:16572-16591 | MS.gene32171:intergenic | 35.0% | |
| GATAAGGAATTGGAAACAGA+TGG | - | 8581:17109-17128 | MS.gene32171:CDS | 35.0% | |
| GGATTTCGGAGAAAACAATA+TGG | + | 8581:16498-16517 | MS.gene32171:intergenic | 35.0% | |
| TCAAATGAGGATATCATGGA+GGG | - | 8581:16907-16926 | MS.gene32171:CDS | 35.0% | |
| TCCAAAAAAATTGGATGCTC+AGG | - | 8581:16838-16857 | MS.gene32171:CDS | 35.0% | |
| TCGCTGAATTCAATTAGTAG+TGG | - | 8581:16575-16594 | MS.gene32171:CDS | 35.0% | |
| TGAAATTCAGAAAGACTACC+TGG | + | 8581:16461-16480 | MS.gene32171:intergenic | 35.0% | |
| TGCAGTTCAGATTTACATGT+TGG | - | 8581:16626-16645 | MS.gene32171:CDS | 35.0% | |
| TGTTTCCAATTCCTTATCCT+CGG | + | 8581:17107-17126 | MS.gene32171:intergenic | 35.0% | |
| TTACAATTGGGTGAGGATTT+CGG | + | 8581:16512-16531 | MS.gene32171:intergenic | 35.0% | |
| ! | CTTAGTTCTCCAACTAGAAA+GGG | - | 8581:16236-16255 | MS.gene32171:CDS | 35.0% |
| !! | ATTTTCTCTCCCTTTCTAGT+TGG | + | 8581:16248-16267 | MS.gene32171:intergenic | 35.0% |
| !! | TCTAGTTCTACAATAGAGGA+TGG | - | 8581:16875-16894 | MS.gene32171:CDS | 35.0% |
| !!! | TCCTGAGCATCCAATTTTTT+TGG | + | 8581:16842-16861 | MS.gene32171:intergenic | 35.0% |
| ACTAAAGCTTCAGTTGGCAA+AGG | + | 8581:16209-16228 | MS.gene32171:intergenic | 40.0% | |
| CAATGCACTAAAGCTTCAGT+TGG | + | 8581:16215-16234 | MS.gene32171:intergenic | 40.0% | |
| CTCAAATGAGGATATCATGG+AGG | - | 8581:16906-16925 | MS.gene32171:CDS | 40.0% | |
| CTTCATACCATTAGCAGTAC+CGG | + | 8581:16414-16433 | MS.gene32171:intergenic | 40.0% | |
| CTTGTAGAGTCCATAACTCT+TGG | - | 8581:16293-16312 | MS.gene32171:CDS | 40.0% | |
| GAAATCCTCACCCAATTGTA+AGG | - | 8581:16511-16530 | MS.gene32171:CDS | 40.0% | |
| GAATTAACTGAATCCTCCAG+TGG | + | 8581:16761-16780 | MS.gene32171:intergenic | 40.0% | |
| GAATTGGAAACAGATGGCAT+TGG | - | 8581:17115-17134 | MS.gene32171:CDS | 40.0% | |
| GATCTGTCTGCCTTACAATT+GGG | + | 8581:16524-16543 | MS.gene32171:intergenic | 40.0% | |
| GTAGAACTAGATAACCAAGG+AGG | + | 8581:16866-16885 | MS.gene32171:intergenic | 40.0% | |
| GTGGCAAAGAACTATTGAGA+AGG | + | 8581:16742-16761 | MS.gene32171:intergenic | 40.0% | |
| TCTCAATAGTTCTTTGCCAC+TGG | - | 8581:16742-16761 | MS.gene32171:CDS | 40.0% | |
| TGAAACCGAGGATAAGGAAT+TGG | - | 8581:17099-17118 | MS.gene32171:CDS | 40.0% | |
| TGATCTGTCTGCCTTACAAT+TGG | + | 8581:16525-16544 | MS.gene32171:intergenic | 40.0% | |
| TTAATCAGTGCTTGTGGACT+TGG | + | 8581:17161-17180 | MS.gene32171:intergenic | 40.0% | |
| TTGGCAAAGGCAACAATGAA+GGG | + | 8581:16196-16215 | MS.gene32171:intergenic | 40.0% | |
| ! | AGTACTGGAACCAAGAGTTA+TGG | + | 8581:16306-16325 | MS.gene32171:intergenic | 40.0% |
| ! | AGTGATAGCAGTGATTTGTC+CGG | - | 8581:16392-16411 | MS.gene32171:CDS | 40.0% |
| ! | CAACTGAAGCTTTAGTGCAT+TGG | - | 8581:16213-16232 | MS.gene32171:CDS | 40.0% |
| ! | CAGAATCGCAAGTCTGAAAT+TGG | - | 8581:16269-16288 | MS.gene32171:CDS | 40.0% |
| ! | GCTTAGTTCTCCAACTAGAA+AGG | - | 8581:16235-16254 | MS.gene32171:CDS | 40.0% |
| ACAACGACTGTGGTAGCAAT+AGG | - | 8581:17008-17027 | MS.gene32171:CDS | 45.0% | |
| AGCGCATGCAGACAATCAAA+AGG | - | 8581:16658-16677 | MS.gene32171:CDS | 45.0% | |
| ATGAGGATATCATGGAGGGA+AGG | - | 8581:16911-16930 | MS.gene32171:CDS | 45.0% | |
| CAACGACTGTGGTAGCAATA+GGG | - | 8581:17009-17028 | MS.gene32171:CDS | 45.0% | |
| CAATAGTTCTTTGCCACTGG+AGG | - | 8581:16745-16764 | MS.gene32171:CDS | 45.0% | |
| GATTTGTCCGGTACTGCTAA+TGG | - | 8581:16404-16423 | MS.gene32171:CDS | 45.0% | |
| GTTGGCAAAGGCAACAATGA+AGG | + | 8581:16197-16216 | MS.gene32171:intergenic | 45.0% | |
| TCTGAACTGCAATCTGACTG+TGG | + | 8581:16617-16636 | MS.gene32171:intergenic | 45.0% | |
| TGAGGATATCATGGAGGGAA+GGG | - | 8581:16912-16931 | MS.gene32171:CDS | 45.0% | |
| ! | ACTGGATGATGGTGAAGTAC+TGG | + | 8581:16321-16340 | MS.gene32171:intergenic | 45.0% |
| ! | TGTGGTAGCAATAGGGTATC+AGG | - | 8581:17016-17035 | MS.gene32171:CDS | 45.0% |
| ACTCGCTGAAACCGAGGATA+AGG | - | 8581:17093-17112 | MS.gene32171:CDS | 50.0% | |
| GCTACCACAGTCGTTGTCAT+CGG | + | 8581:17005-17024 | MS.gene32171:intergenic | 50.0% | |
| GTCTGCCTTACAATTGGGTG+AGG | + | 8581:16519-16538 | MS.gene32171:intergenic | 50.0% | |
| CTCTCCGATGACAACGACTG+TGG | - | 8581:16998-17017 | MS.gene32171:CDS | 55.0% | |
| GCGAGTCCCCCATCATAGTT+TGG | + | 8581:17079-17098 | MS.gene32171:intergenic | 55.0% | |
| TGGATGCTCAGGATCCTCCT+TGG | - | 8581:16849-16868 | MS.gene32171:CDS | 55.0% | |
| TGGGGGACTCGCTGAAACCG+AGG | - | 8581:17087-17106 | MS.gene32171:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 8581 | gene | 16170 | 17180 | 16170 | ID=MS.gene32171 |
| 8581 | mRNA | 16170 | 17180 | 16170 | ID=MS.gene32171.t1;Parent=MS.gene32171 |
| 8581 | exon | 16170 | 17180 | 16170 | ID=MS.gene32171.t1.exon1;Parent=MS.gene32171.t1 |
| 8581 | CDS | 16170 | 17180 | 16170 | ID=cds.MS.gene32171.t1;Parent=MS.gene32171.t1 |
| Gene Sequence |
| Protein sequence |
